2.3.1.178 - 660740 2.3.1.178 cloned into the expression vector pQE30 with a 6×histidine tag and expressed in Escherichia coli JM109 M15 cells 756904 2.3.1.178 expression in Escherichia coli 660836 2.3.1.178 expression in Escherichia coli with an additional six His residues at the C-terminus 661224 2.3.1.178 expression in Escherichia coli, also osmoregulated in Escherichia coli 662773 2.3.1.178 expression of the His-tagged enzyme in Escherichia coli strain BL21 (DE3) 720373 2.3.1.178 gene ectA, ectABC operon, in Marinococcus halophilus, the transcription of ectABC genes is initiated from three individual sigma70/sigmaA-promoters locate upstream of each gene 718536 2.3.1.178 gene ectA, encoded in ectABC, transcription of the ectABC-ask operon is initiated from two sigma70-dependent promoters ectAp1 and ectAp2. Gene ectR1 encodes the MarR-like transcriptional regulator EctR that is involved in the regulation of ectABC, overview 718536 2.3.1.178 heterologous production of EctA in Escherichia coli, with either a N-terminal (NH2-WSHPQFEK-SG) or a C-terminal (SA-WSHPQFEK-COOH) Strep-Tag II peptide for their purification by affinity chromatography 757238 2.3.1.178 into the pET22b+ vector for expression in Escherichia coli BL21DE3 cells 686806 2.3.1.178 our putative promoters (?ectA1-4) are located upstream of the ectA gene and one internal promoter PectB is upstream of the ectB gene 718536