1.3.1.42 - 390776 1.3.1.42 c-DNA library, gene OPR3 identified 390778 1.3.1.42 cDNA library construction, protein S64, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain BL21(DE3) as His-tagged protein 656976 1.3.1.42 cloned and expressed in Escherichia coli 724151 1.3.1.42 cloning and expression of a gene encoding old yellow enzyme 390778 1.3.1.42 cloning, and DNA sequence determination and analysis, expression of gene At2g06050, encoding isozyme OPR3, in Escherichia coli as N-terminally fusion protein fused to S-tag-His6-maltose binding protein 657324 1.3.1.42 construction of a cDNA library, phylogenetic analysis, DNA and amino acid sequence determination and analysis, expression as His-tagged enzyme in Escherichia coli strain BL21(DE3) 657134 1.3.1.42 construction of a genomic DNA library, identification and DNA sequence comparison of 6 genes of OPR belonging to the OPR enzyme family subgroup I, which is not required for jasmonate biosynthesis, DNA sequence and gene structure determination and analysis, expression of isozymes in Escherichia coli strain BL21(DE3) 656802 1.3.1.42 construction of a genomic DNA library, identification of 6 genes of OPR belonging to the OPR enzyme family subgroup I, which is not required for jasmonate biosynthesis, DNA sequence and gene structure determination and analysis, expression of isozymes in Escherichia coli strain BL21(DE3) 656802 1.3.1.42 enzyme cloned from its cDNA, open reading frame encodes a 372 amino acids polypeptide, functionally expressed in Escherichia coli 390775 1.3.1.42 expressed in Arabidopsis thaliana through transformation with Agrobacterium tumefaciens strain EHA105 689685 1.3.1.42 expressed in Arabidopsis thaliana through transformation with Agrobacterium tumefaciens strain GV3101 689661 1.3.1.42 expressed in Escherichia coli 678118, 726148 1.3.1.42 expression in Escherichia coli BL21 763386 1.3.1.42 expression of His-tagged isozyme OPR1 in Escherichia coli, transient expression of isozyme OPR1 as enhanced-GFP- or RFP-fusion protein in onion epidermal cells for subcellular localization study 656998 1.3.1.42 expression of His-tagged isozyme OPR2 in Escherichia coli 656998 1.3.1.42 expression of His-tagged isozyme OPR3 in Escherichia coli, transient expression of isozyme OPR3 as enhanced-GFP- or RFP-fusion protein in onion epidermal cells for subcellular localization study 656998 1.3.1.42 expression of the 3 isozymes OPR1-3 as N-terminally GST- or His-tagged enzymes in Escherichia coli 656998 1.3.1.42 gene CsOPR3, a putative OPR gene, DNA and amino acid sequence determination and analysis, phylogenetic analysis, quantitative real-time PCR expression analysis 745029 1.3.1.42 gene TaOPR1, TaOPR1 maps to the short arm of the second chromosome of the B genome (2BS), DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant overexpression in Arabidopsis thaliana ecotype Col-0. The repression of AtOPR1 by the TaOPR1 transgene suggests a negative feedback regulatory effect, perhaps based on functional redundancy between TaOPR1 and AtOPR1 746104 1.3.1.42 generating of a jasmonic acid-deficient rice line with suppressed expression of the gene encoding OPR3 with resistance to Magnaporthe grisea by introduction into rice plants by Agrobacterium-mediated transformation, OPR-interfering RNA plants show normal resistance to both an incompatible and a compatible race of Magnaporthe grisea 690761 1.3.1.42 generating of a jasmonic acid-deficient rice line with suppressed expression of the genes encoding OPR1 with resistance to Magnaporthe grisea by introduction into rice plants by Agrobacterium-mediated transformation, OPR-interfering RNA plants show normal resistance to both an incompatible and a compatible race of Magnaporthe grisea 690761 1.3.1.42 OPR DNA and amino acid sequence determination and analysis, comparative genomic analysis of OPR genes, phylogenetic analysis and sequence comparisons, detailed overview 711596 1.3.1.42 OPR1 and OPR2, two closely related genes encodes 12-oxophytodienoic acid-10,11-reductases 390778 1.3.1.42 OPR3 expressed in functional form in Escherichia coli, cloning of OPR2 cDNA by reverse transcriptase-polymerase chain reaction, OPR2 expressed in insect cell cultures of Spodoptera frugiperda with transgenic Autographa californica baculoviruses and overexpressed in transgenic Arabidopsis thaliana 390779 1.3.1.42 OPR3 mutant enzymes are expressed in Escherichia coli 699588 1.3.1.42 overexpression of Arabidopsis OPR3 in hexaploid wheat (Triticum aestivum) alters plant development and freezing tolerance. Transgenic wheat plants with high AtOPR3 expression levels are characterized by delayed germination, slower growth, late flowering and senescence, and improved tolerance to short-term freezing 763154 1.3.1.42 recombinant expression in Escherichia coli, RNA interference is used to investigate the effect of knockdown expression of OPR1 700835 1.3.1.42 recombinant expression in Escherichia coli, RNA interference is used to investigate the effect of knockdown expression of OPR2 700835 1.3.1.42 recombinant expression of OPR3 in Escherichia coli 700835 1.3.1.42 subcloning of the OPR genes from a lambdaGEM11 genomic library 390777