4.2.1.96	-	
4.2.1.96	expression in Escherichia coli as GST-tagged enzyme	
4.2.1.96	expression in Escherichia coli BL21 (DE3). There is a high degree of structural similarity between Toxoplasma gondii PCD and the mammalian orthologue, Rattus norvegicus PCD, which is identical to the human enzyme	
4.2.1.96	expression in Escherichia coli BL21(DE3)pLysS	
4.2.1.96	expression in recombinant Escherichia coli strain BL21(DE3) tnaA-, co-expression with Chromobacterium violaceum L-phenylalanine 4-hydroxylase mutant L101Y-W180F, Escherichia coli dihydropteridine reductase, and Bacillus subtilis glucose dehydrogenase genes for installation of 5-hydroxy-L-tryptophan biosynthesis in the recombinant Escherichia coli strain, method optimization and development of a cofactor regeneration system for L-tryptophan hydroxylation for enhanced synthesis of 5-hydroxy-L-tryptophan	
4.2.1.96	gene 100277322, the raf2 locus corresponds to GRMZM2G139123, an intron-less locus on chromosome 1	
4.2.1.96	overexpression in Escherichia coli	
4.2.1.96	PCD sequence comparisons and phylogenetic tree, enzyme expression analysis	
4.2.1.96	plasmids pJS11, which carries phhA, and pBluescript, carrying phhB transformed simultaneously by electroporation into Escherichia coli strains JP2255, JP2255 folM, and JP2255 folX	
4.2.1.96	targeting of full-length GFP-tagged PCD sequence to chloroplasts in Arabidopsis thaliana mesophyll protoplasts	
4.2.1.96	the 18 amino acid-truncated mutant E87T cannot be overexpressed in Escherichia coli	
4.2.1.96	wild type and mutant enzyme C82R overexpressed in Escherichia coli	
4.2.1.96	wild-type and mutant enzymes