2.3.2.23	-	
2.3.2.23	a fragment of the enzyme is cloned into a virus-induced genesilencing vector based on the geminivirus tomato mottlevirus	
2.3.2.23	chromosome localization and gene duplication analysis of VvUBCs, phylogenetic analysis, transcriptome analysis, detailed overview	
2.3.2.23	expresion in Escherichia coli	
2.3.2.23	expressed and transformed into Escherichia coli (BL-21 DE3) using pET22 vector in frame to a C-terminal His6 tag	
2.3.2.23	expression in baculoviral system	
2.3.2.23	expression in Escherichia coli	
2.3.2.23	gene cdc34, recombinant His-tagged enzyme expression in Escherichia coli strain BL21 (DE3) Codon Plus	
2.3.2.23	gene rad6a, the S-Flag-biotin (SFB)-tagged full length E2 is expressed in HEK-293T, HeLa, or U2OS cells, recombinant coexpression of Rad6b with E3 ubiquitin ligases RNF168 and RAD18 in E2-deficient MEF cells leading to formation of ionizing radiation-induced foci (IRIF)	
2.3.2.23	gene rad6b, the S-Flag-biotin (SFB)-tagged full length E2 is expressed in HEK-293T, HeLa, or U2OS cells, recombinant coexpression of Rad6b with E3 ubiquitin ligases RNF168 and RAD18 in E2-deficient MEF cells leading to formation of ionizing radiation-induced foci (IRIF)	
2.3.2.23	gene UBE2K, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli	
2.3.2.23	gene UBE2R1 or CDC34, recombinant expression of GST- or His6-tagged enzyme in Escherichia coli strain Rosetta (DE3)	
2.3.2.23	gene UBE2T, genotyping	
2.3.2.23	gene UBE2T, located in chromosome 1q32.1, quantitative real-time PCR enzyme expression analysis	
2.3.2.23	gene UBE2Z, sequence comparisons, recombinant overexpression of His6-tagged enzyme in Escherichia coli strain BL21(DE3)	
2.3.2.23	subcloned in Escherichia coli BL21(DE2), via pET28 vector, N-terminally His-tagged