2.3.2.23	gene UBE2K, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli	
2.3.2.23	gene UBE2R1 or CDC34, recombinant expression of GST- or His6-tagged enzyme in Escherichia coli strain Rosetta (DE3)	
2.3.2.23	gene UBE2T, genotyping	
2.3.2.23	gene UBE2T, located in chromosome 1q32.1, quantitative real-time PCR enzyme expression analysis	
2.3.2.23	gene UBE2Z, sequence comparisons, recombinant overexpression of His6-tagged enzyme in Escherichia coli strain BL21(DE3)	
2.3.2.23	subcloned in Escherichia coli BL21(DE2), via pET28 vector, N-terminally His-tagged