2.3.1.255 - 2.3.1.255 a plasmid expressing hNaa16p, NARG1L-FLAG, is used 2.3.1.255 cloning of V5-tagged hNaa15p, hNaa25p, and hNaa35p from HEK-293 cell genomic DNA, expression of His-tagged and maltose binding protein-tagged subunits hNaa15p, hNaa10p, and hNaa50p in Escherichia coli, efficient expression of MBP-hNaa15p requires coexpression of pDC952, a plasmid carrying the Escherichia coli argU gene 2.3.1.255 expressed in Escherichia coli BL21 cells 2.3.1.255 expressed in Escherichia coli BL21(DE3) cells 2.3.1.255 expressed in Escherichia coli BL21(DE3)pLysS cells 2.3.1.255 expressed in Escherichia coli Rosetta (DE3)pLysS cells 2.3.1.255 expressed in Escherichia coli Rosetta II(DE3)pLysS cells 2.3.1.255 expressed in Escherichia coli Rosetta Star cells 2.3.1.255 expression in HeLa cells 2.3.1.255 gene NAA10, DNA and amino acid sequence determination and analysis, genotyping, recombinant expression of His-MBP-tagged wild-type and mutant Naa10 in Escherichia coli strain BL21 Star DE3 2.3.1.255 gene NAA10, genotyping, recombinant expression of His-MBP-tagged wild-type and mutant Naa10 in Escherichia coli and in HeLa cells 2.3.1.255 gene NAA10, genotyping, sequence comparisons, enzyme expression analysis 2.3.1.255 gene NAA10, real-time reverse transcription-PCR enzyme expression analysis 2.3.1.255 gene NAA10, recombinant expression of His-tagged ARD1/Naa10 in Escherichia coli strain BL21, recombinant expression of GST-tagged enzyme in Escherichia coli strain BL21 2.3.1.255 gene NAA10, recombinant expression of the enzyme in human 293T cells using a lentiviral vector, recombinant expression of GST-tagged wild-type and mutant Naa10 enzymes in Escherichia coli strain BL21 2.3.1.255 gene rimI, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) 2.3.1.255 gene Rv3420c or rimI, recombinant expression of C-terminally His6-tagged enzyme in Escherichia coli 2.3.1.255 genes NAA10 and NAA15, recombinant enzyme complex NatA expression in Spodoptera frugiperda SF9 cells via transfection using the baculovirus system 2.3.1.255 genes TcNaa15 and TcNaa10, DNA and amino acid sequence determination, comparison, and analysis 2.3.1.255 recombinant expression of His-tagged hARD1/NAA10 2.3.1.255 recombinant expression of N-terminally His-tagged NatA in Spodoptera frugiperda Sf9 cells, coexpression of tagged HYPK