1.2.1.31	-	
1.2.1.31	cDNA is cloned from a human fetal library, library is constructed in a modified pBluescript II SK+ vector, cDNA insert is sequenced	
1.2.1.31	cloning of lys2, the cloned gene encodes a functional alpha-aminoadipate reductase as shown by complementation of a Penicillium chrysogenum lys2 mutant, the aminoacid sequence shows strong homology with the alpha-aminoadipate reductase encoded by lys2 genes of Candida albicans, Schizosaccharomyces pombe, Saccharomyces cerevisiae and Penicillium chrysogenum	
1.2.1.31	cloning of the LYS2 gene	
1.2.1.31	encoded by two different genes, LYS2 and LYS5	
1.2.1.31	expression in Escherichia coli as an active enzyme. Exhibits significant alpha-aminoadipate reductase activity without the addition of CoA and Lys7p PPTase from Schizosaccharomyces pombe	
1.2.1.31	heterlogous expressed in Escherichia coli	
1.2.1.31	LYS2 gene cloned	
1.2.1.31	LYS2 gene codes for alpha-aminoadipate reductase, construction and use of LYS2 cartridges	
1.2.1.31	LYS2 gene codes for alpha-aminoadipate reductase, LYS2 genes encodes a protein with 49.9% identity to the Saccharomyces cerevisiae, 51.3% identity to the Schizosaccharomyces pombe and 48.12% identity to the Candida albicans alpha-aminoadipate reductases	
1.2.1.31	LYS2 gene codes for alpha-aminoadipate reductase, LYS2 ORF exhibits 63% identity at the nucleotide level and 56.2% identity at the aminoacid level to the LYS2 gene of Saccharomyces cerevisiae	
1.2.1.31	Lys5 and Lys2 cloned from Saccharomyces cerevisiae DNA, overexpressed in Escherichia coli, and purified, Lys5/Lys2 pair is a two component system in which Lys5 covalently primes Lys2, allowing alpha-aminoadipate reductase activity by holo-Lys2 with catalytic cycles of autoaminoacylation and reductive clevage	
1.2.1.31	one-step homologous recombination in Saccharomyces cerevisiae, expression of the human gene in the yeast rescues the lys5 knockout phenotype	
1.2.1.31	two genes, lys1+ and lys7+ are required for the synthesis of the enzyme, some regions of the enzyme sequence are similar to the sequence of Saccharomyces cerevisiae, e.g. Schizosaccharomyces pombe enzyme residues 345 through 385, and 449 through 470