4.2.1.119 ? x * 17500, recombinant isozyme His-tagged PhaJ4aRe, SDS-PAGE, x * 17000, recombinant His-tagged PhaJ4bRe, SDS-PAGE, x * 16500, recombinant His-tagged PhaJ4cRe, SDS-PAGE -, 729029 4.2.1.119 ? x * 31500-44000 684455 4.2.1.119 ? x * 34000, calculated from sequence 680715 4.2.1.119 ? x * 45000, HsMFE-2(dhdelta), HsMFE-2(dhdelta, E366A), HsMFE-2(dhdelta, D510A), SDS-PAGE 652075 4.2.1.119 ? x * 63000, both recombinant CtMfe2p(dha+bdelta) and its SeMet analogue, SDS-PAGE 649201 4.2.1.119 ? x * 80000, SDS-PAGE -, 715528 4.2.1.119 dimer 2 * 13954, calculated from sequence 33736 4.2.1.119 dimer 2 * 64100, MFE-2, SDS-PAGE 714098 4.2.1.119 homodimer 2 * 31500, microsomal isoform, SDS-PAGE 5870 4.2.1.119 homodimer 2 * 33000, SDS-PAGE 697692 4.2.1.119 homodimer 2 * 33500, peroxisomal isoform, SDS-PAGE 5870 4.2.1.119 monomer size-exclusion chromatography on a Superdex 200 HR column gives a native molecular mass of 59 kDa, suggesting that the recombinant protein is monomeric 684952 4.2.1.119 additional information MFE2 consists of a (3R)-hydroxyacyl-CoA dehydrogenase, HD, domain, a (2E)-enoyl-CoA hydratase 2, H2, domain, and a sterol carrier protein 2-like domain -, 715528 4.2.1.119 additional information necessity of dimerization, domain organization, MFE-2 structure-function studies, overview 714098