4.1.3.27 ? x * 54000, SDS-PAGE -, 746626 4.1.3.27 ? x * 60000-65000 + x + 60000-65000, Asalpha-subunit and Asbeta-subunit are of a similar MW 33307 4.1.3.27 ? x * 64512, calculation from nucleotide sequence 666679 4.1.3.27 ? x * 65013, calculation from nucleotide sequence 666679 4.1.3.27 dimer - 33283 4.1.3.27 dimer 1 * 14000 + 1 * 70000, gel filtration or sucrose density gradient centrifugation after separation of subunits 33282 4.1.3.27 dimer 1 * 16000 + 1 * 80000 33283 4.1.3.27 dimer 1 * 18000 + 1 * 63400, sucrose density gradient centrifugation 33281 4.1.3.27 dimer 1 * 18000 + 1 * 64000 33283 4.1.3.27 dimer 1 * 28000 + 1 * 72000, gel filtration after separation of subunits -, 33293 4.1.3.27 dimer 1 * 37000 + 1 * 60000, isoenzyme AS-b, SDS-PAGE 33301 4.1.3.27 heterodimer 1 * 57000 + 1 * 23000, SDS-PAGE, 1 * 48500 + 1 * 21100, deduced from gene sequence 649565 4.1.3.27 heterodimer alpha, TrpE, and beta, PabA, subunit 692505 4.1.3.27 heterotetramer - 746743 4.1.3.27 heterotetramer 2 * 47743 + 2 * 21913, deduced from amino acid sequence -, 677906 4.1.3.27 heterotetramer 2 * ? + 2 * 32000, SDS-PAGE 747926 4.1.3.27 heterotetramer association of 2 * enzyme + 2 * glutamine amidotransferase, crystallographic data -, 653636 4.1.3.27 heterotetramer crystallographic data 653318 4.1.3.27 monomer 1 * 66600, laser light scattering 748650 4.1.3.27 monomer 1 * 67000, SDS-PAGE 748650 4.1.3.27 monomer 1 * 79000, SDS-PAGE -, 33289 4.1.3.27 monomer crystallographic data 653835 4.1.3.27 additional information component I has a MW of 63000 Da, gel filtration 33277 4.1.3.27 additional information glutamine, 10 mM, enhances binding of AS I and II, as does Mg2+. ASI and ASII play a joint role in the binding of L-Gln, negligible binding to either component alone 33281 4.1.3.27 additional information multifunctional anthranilate synthetase-anthranilate 5-phosphoribosylpyrophosphate phosphoribosyltransferase 33279 4.1.3.27 additional information multifunctional enzyme anthranilate synthase/N(5'-phosphoribosyl)anthranilate synthase, EC 4.1.3.27/EC 2.4.2.18, or ASI/ASII. ASI, is encoded by the gene TrpE and catalyzes the formation of anthranilate from chorismate and NH4+ as an independent subunit and from chorismate and Gln, when complexed with ASII. ASII consists of a glutamine amidotransferase domain, TrpG, which enables the complex to utilize Gln as an NH4+ source and an N-(5'-phosphoribosyl)anthranilate synthase domain, which catalyzes the production of N-(5'-phosphoribosyl)anthranilate from anthranilate and (5-phosphoribosyl)anthranilate 33304 4.1.3.27 additional information one subunit is a trifunctional peptide which contains the catalytic sites for phosphoribosylanthranilate isomerase and indoleglycerol phosphate synthetase reactions, and associates with the second subunit to form Gln-dependent anthranilate synthetase 4604 4.1.3.27 additional information partial complex anthranilate synthase consists of two TrpE:TrpG heterodimers 715843 4.1.3.27 additional information recombinant alpha-subunit interacts with native beta-subunit to form an active enzyme 653510 4.1.3.27 additional information subunit ASI catalyzes NH4+-dependent synthesis of anthranilate. Gln is not a substrate for ASI. ASII provides Gln-amide transfer function -, 33298 4.1.3.27 additional information synthase subunit TrpE and glutaminase subunit TrpG 718925 4.1.3.27 additional information the 72000 Da subunit converts chorismate to anthranilate using NH4+. The 28000 Da subunit confers the enzyme the ability to use Gln instead of NH4+ as substrate -, 33293 4.1.3.27 additional information the large component from Bacillus subtilis (IB) complements well with the small component from Pseudomonas aeruginosa (IIP) to reconstitute a glutamine-reactive anthranilate synthase. Complementation was also observed with the large component from Pseudomonas aeruginosa (Iv) and the small subunit from Bacillus subtilis (IIB) 665348 4.1.3.27 additional information the organism contains an NH4-dependent enzyme, i.e. component I, and a Gln-dependent enzyme that is composed of 2 NH4+-dependent subunits and of 2 amidotransferase subunits, i.e. I2II2 33291 4.1.3.27 tetramer - 33279, 33283, 33284 4.1.3.27 tetramer 2 * 18000 + 2 * 71000 33283 4.1.3.27 tetramer 2 * 20000 + 2 * 50000, Gln-dependent enzyme, composed of 2 NH4+-dependent subunits and of 2 amidotransferase subunits, I2II2, gel filtration 33291 4.1.3.27 tetramer 2 * 20956 + 2 * 60000 -, 33298 4.1.3.27 tetramer 2 * 25500 + 2 * 67000, SDS-PAGE 33305 4.1.3.27 tetramer 2 * 76000 + 2 * 84000, alpha2,beta2, SDS-PAGE 33296 4.1.3.27 tetramer alpha2,beta2, 2 * 70000 + 2 * 94000, SDS-PAGE 4604 4.1.3.27 tetramer alpha2beta2, 2 * 18000 + 2 * 50000, SDS-PAGE 664871 4.1.3.27 tetramer ASI, ASII, 2 * 21000 + 2 * 60000, SDS-PAGE 33279, 33283