6.3.1.11	ATP + glutamate + putrescine	the enzyme is involved in a putrescine utilization pathway	Escherichia coli	ADP + phosphate + gamma-L-glutamylputrescine	-	?	375064	
6.3.1.11	ATP + L-glutamate + putrescine	-	Escherichia coli	ADP + phosphate + gamma-L-glutamylputrescine	-	?	376362	
6.3.1.11	ATP + L-glutamate + putrescine	the enzyme catalyzes the gamma-glutamylation of putrescine, the first step in a different putrescine utilization pathway involving gamma-glutamylated intermediates, the Puu pathway, in Escherichia coli, overview. The intracellular concentration of putrescine is probably optimized by PuuA transcriptionally and posttranslationally and excess putrescine is converted to a nutrient source by the Puu pathway, regulation, overview	Escherichia coli K-12	ADP + phosphate + gamma-L-glutamylputrescine	-	?	376362	
6.3.1.11	ATP + L-glutamate + putrescine	PuuA exhibits the highest activity for putrescine, which has four carbon atoms, and activity drops when the methylene chain of diamine is longer or shorter than C4	Escherichia coli K-12	ADP + phosphate + gamma-L-glutamylputrescine	-	?	376362	
6.3.1.11	additional information	intracellular polyamine profile of various strains relevant to puuA, overview	Escherichia coli K-12	?	-	?	89	
6.3.1.11	additional information	when any one of the substrates and a cofactor , i.e. putrescine, glutamate, ATP, and the metal ion, are omitted from the reaction mixture, gamma-Glu-Put is not formed	Escherichia coli K-12	?	-	?	89