4.2.3.165 (2E,6E)-farnesyl diphosphate - Fusarium proliferatum (1R,4R,5S)-(-)-guaia-6,10(14)-diene + diphosphate - ? 438009 4.2.3.165 (2E,6E)-farnesyl diphosphate - Fusarium mangiferae (1R,4R,5S)-(-)-guaia-6,10(14)-diene + diphosphate - ? 438009 4.2.3.165 (2E,6E)-farnesyl diphosphate - Fusarium fujikuroi (1R,4R,5S)-(-)-guaia-6,10(14)-diene + diphosphate - ? 438009 4.2.3.165 (2E,6E)-farnesyl diphosphate no activity with geranyl diphosphate and geranylgeranyl diphosphate Fusarium proliferatum (1R,4R,5S)-(-)-guaia-6,10(14)-diene + diphosphate (1R,4R,5S)-(-)-guaia-6,10(14)-diene i.e. (1R)-1-methyl-4-methylidene-7-(propan-2-yl)-1,2,3,3a,4,5,6,8a-octahydroazulene i.e. (1R)-7-isopropyl-1-methyl-4-methylene-1,2,3,3a,4,5,6,8a-octahydroazulene ? 438009 4.2.3.165 (2E,6E)-farnesyl diphosphate no activity with geranyl diphosphate and geranylgeranyl diphosphate Fusarium mangiferae (1R,4R,5S)-(-)-guaia-6,10(14)-diene + diphosphate (1R,4R,5S)-(-)-guaia-6,10(14)-diene i.e. (1R)-1-methyl-4-methylidene-7-(propan-2-yl)-1,2,3,3a,4,5,6,8a-octahydroazulene i.e. (1R)-7-isopropyl-1-methyl-4-methylene-1,2,3,3a,4,5,6,8a-octahydroazulene ? 438009 4.2.3.165 (2E,6E)-farnesyl diphosphate the original STC5 from Fusarium fujikuroi is inactive because of a critically naturally occuring mutation at position 288 in STC5 that leads to an asparagine to lysine exchange in the NSE triad. Sequence correction by site-directed mutagenesis (K288N) restores activity. No activity with geranyl diphosphate and geranylgeranyl diphosphate Fusarium fujikuroi (1R,4R,5S)-(-)-guaia-6,10(14)-diene + diphosphate (1R,4R,5S)-(-)-guaia-6,10(14)-diene = (1R)-1-methyl-4-methylidene-7-(propan-2-yl)-1,2,3,3a,4,5,6,8a-octahydroazulene i.e. (1R)-7-isopropyl-1-methyl-4-methylene-1,2,3,3a,4,5,6,8a-octahydroazulene ? 438009 4.2.3.165 (2E,6E)-farnesyl diphosphate - Fusarium fujikuroi IMI 58289 (1R,4R,5S)-(-)-guaia-6,10(14)-diene + diphosphate - ? 438009 4.2.3.165 (2E,6E)-farnesyl diphosphate the original STC5 from Fusarium fujikuroi is inactive because of a critically naturally occuring mutation at position 288 in STC5 that leads to an asparagine to lysine exchange in the NSE triad. Sequence correction by site-directed mutagenesis (K288N) restores activity. No activity with geranyl diphosphate and geranylgeranyl diphosphate Fusarium fujikuroi IMI 58289 (1R,4R,5S)-(-)-guaia-6,10(14)-diene + diphosphate (1R,4R,5S)-(-)-guaia-6,10(14)-diene = (1R)-1-methyl-4-methylidene-7-(propan-2-yl)-1,2,3,3a,4,5,6,8a-octahydroazulene i.e. (1R)-7-isopropyl-1-methyl-4-methylene-1,2,3,3a,4,5,6,8a-octahydroazulene ? 438009