3.4.24.B11 aggrecan + H2O - ? - ? 15923 3.4.24.B11 aggrecan + H2O C-terminal spacer region of the enzyme is required for activity, substrate is a cartilage proteoglycan, cleavage site is the peptide bond Glu1871-Leu1872 within the substrates chondroitin sulfate attachment domain ? - ? 15923 3.4.24.B11 aggrecan + H2O substrate is a cartilage proteoglycan ? - ? 15923 3.4.24.B11 aggrecan + H2O in 50 mM Tris-HCl, pH 7.6, 150 mM NaCl and 5mM CaCl2 solution with 100 ng recombinant ADAMTS-1 at 37°C ? - ? 15923 3.4.24.B11 aggrecan + H2O 2mg/ml, assay at 37°C, 16 h ? - ? 15923 3.4.24.B11 aggrecan + H2O several cleavage sites are determined 11 aggrecan fragment product determination, overview ? 367543 3.4.24.B11 aggrecan + H2O cleavage site is Glu373-Ala374 2 aggrecan fragment - ? 367546 3.4.24.B11 alpha2-macroglobulin + H2O cleavage of the bait region of alpha2-macroglobulin, complex formation with alpha2-macroglobulin is dependent on the proteolytic activity ? - ? 37183 3.4.24.B11 biglycan + H2O biglycan is a small leucine-rich proteoglycan ? - ? 391508 3.4.24.B11 brevican + H2O clevage site is Glu395 ? - ? 367547