3.4.21.99 additional information involved in ascidian fertilization Halocynthia roretzi ? - ? 89 3.4.21.99 additional information the enzyme binds to the vitelline coat plays a key role in sperm penetration through the vitelline coat during ascidian fertilization Halocynthia roretzi ? - ? 89 3.4.21.99 additional information the enzyme plays an essential role in fertilization of Halocynthia roretzi Halocynthia roretzi ? - ? 89 3.4.21.99 additional information the enzyme preferentially or specifically splits the Val-Pro-/-Arg bond in synthetic fluorogenic substrates. The enzyme prefers the Pro residue to Leu, Ser and Gly in the P2 position and prefers the Val residue to Leu, Phe and Gly in the p3 position Halocynthia roretzi ? - ? 89 3.4.21.99 tert-butyloxycarbonyl-Val-Pro-Arg-4-methylcoumarin 7-amide + H2O - Halocynthia roretzi tert-butyloxycarbonyl-Val-Pro + Arg-4-methylcoumarin-7-amide - ? 95008