2.3.2.B8	additional information	the enzyme is autoinhibited by an intramolecular interaction between its HECT (homologous to the E6-AP carboxy terminus domain) and two central WW domains. Ndfip1 binds these WW domains to release the HECT, allowing trans-thiolation and enzyme catalytic activity	Mus musculus	?	-	?	89	
2.3.2.B8	additional information	the full-length UBE2E proteins primarily promote protein monoubiquitylation, whereas the core UBC domain of each can efficiently build polyubiquitin chains	Homo sapiens	?	-	?	89	
2.3.2.B8	additional information	the N-terminal extension of Ube2E1 undergoes intramolecular auto-ubiquitination. This self-ubiquitination activity is enhanced in the presence of interacting RING E3 ligases like RNF4 and results in a progressive attenuation of the E2 activity toward substrate/E3 modification	Homo sapiens	?	-	?	89	
2.3.2.B8	S-ubiquitinyl-[ubiquitin-carrier protein E2]-L-cysteine + [HECT-E3-ubiquitin-carrier protein cIAP2]-L-cysteine	-	Homo sapiens	[ubiquitin-carrier protein E2]-L-cysteine + S-ubiquitinyl-[HECT-E3-ubiquitin-carrier protein cIAP2]-L-cysteine	-	?	435823	
2.3.2.B8	S-ubiquitinyl-[ubiquitin-carrier protein E2]-L-cysteine + [HECT-E3-ubiquitin-carrier protein]-L-cysteine	-	Homo sapiens	[ubiquitin-carrier protein E2]-L-cysteine + S-ubiquitinyl-[HECT-E3-ubiquitin-carrier protein]-L-cysteine	-	?	434804	
2.3.2.B8	S-ubiquitinyl-[ubiquitin-carrier protein E2]-L-cysteine + [HECT-E3-ubiquitin-carrier protein]-L-cysteine	-	Mus musculus	[ubiquitin-carrier protein E2]-L-cysteine + S-ubiquitinyl-[HECT-E3-ubiquitin-carrier protein]-L-cysteine	-	?	434804	
2.3.2.B8	[ubiquitin-carrier protein UbcH5]-S-ubiquitinyl-L-cysteine + [HECT-E3-ubiquitin-carrier protein E6-AP]-L-cysteine	a region of isoform UbcH5 encompassing the catalytic site cysteine residue is critical for its ability to interact with E3 enzymes E6-AP and RSP5. Of particular importance is a phenylalanine residue at position 62 of UbcH5 that is conserved among the members of the UBC4/UBC5 subfamily but is not present in any of the other known E2s	Homo sapiens	[ubiquitin-carrier protein UbcH5]-L-cysteine + [HECT-E3-ubiquitin-carrier protein E6-AP]-S-ubiquitinyl-L-cysteine	-	?	429064	
2.3.2.B8	[ubiquitin-carrier protein UbcH5]-S-ubiquitinyl-L-cysteine + [HECT-E3-ubiquitin-carrier protein Rsp5]-L-cysteine	a region of isoform UbcH5 encompassing the catalytic site cysteine residue is critical for its ability to interact with E3 enzymes E6-AP and RSP5. Of particular importance is a phenylalanine residue at position 62 of UbcH5 that is conserved among the members of the UBC4/UBC5 subfamily but is not present in any of the other known E2s	Homo sapiens	[ubiquitin-carrier protein UbcH5]-L-cysteine + [HECT-E3-ubiquitin-carrier protein Rsp5]-S-ubiquitinyl-L-cysteine	-	?	429065