2.1.1.99 additional information hydrogenation of 2,3 double bond of tabersonine instead of hydration results in a 75% decrease in N-methylation, double bound present: no N-methylation, the presence of 6,7 double bound is a necessary structural requirement for N-methylation with membrane-bound enzyme, whereas the detergent-solubilized enzyme is also able to N-methylate the 6,7 anhydro analogue Catharanthus roseus ? - ? 89 2.1.1.99 S-adenosyl-L-methionine + 16-methoxy-2,3-dihydro-3-hydroxytabersonine - Catharanthus roseus S-adenosyl-L-homocysteine + deacetoxyvindoline i.e. N(1)-methyl-16-methoxy-2,3-dihydro-3-hydroxytabersonine ? 320026 2.1.1.99 S-adenosyl-L-methionine + 16-methoxy-2,3-dihydro-3-hydroxytabersonine high degree of specificity for the N-indole of 2,3-dihydro-3-hydroxytabersonine Catharanthus roseus S-adenosyl-L-homocysteine + deacetoxyvindoline - ? 320026 2.1.1.99 S-adenosyl-L-methionine + 16-methoxy-2,3-dihydro-3-hydroxytabersonine enzyme is involved in the biosynthesis of vindoline from tabersonine Catharanthus roseus S-adenosyl-L-homocysteine + deacetoxyvindoline - ? 320026