6.1.2.1	D-Ala + D-2-hydroxybutanoate + ATP	-	Escherichia coli	D-Ala-D-2-hydroxybutanoate + ADP + phosphate	-	?	411921	
6.1.2.1	D-Ala + D-2-hydroxybutanoate + ATP	-	Enterococcus faecalis	D-Ala-D-2-hydroxybutanoate + ADP + phosphate	-	?	411921	
6.1.2.1	D-Ala + D-2-hydroxybutanoate + ATP	-	Enterococcus faecium	D-Ala-D-2-hydroxybutanoate + ADP + phosphate	-	?	411921	
6.1.2.1	D-Ala + D-2-hydroxybutanoate + ATP	-	Enterococcus faecium BM4147	D-Ala-D-2-hydroxybutanoate + ADP + phosphate	-	?	411921	
6.1.2.1	D-Ala + D-2-hydroxyvalerate + ATP	-	Enterococcus faecalis	D-Ala-D-2-hydroxyvalerate + ADP + phosphate	-	?	411922	
6.1.2.1	D-Ala + D-2-hydroxyvalerate + ATP	-	Enterococcus faecium	D-Ala-D-2-hydroxyvalerate + ADP + phosphate	-	?	411922	
6.1.2.1	D-Ala + D-2-hydroxyvalerate + ATP	-	Enterococcus faecium BM4147	D-Ala-D-2-hydroxyvalerate + ADP + phosphate	-	?	411922	
6.1.2.1	D-Ala + D-Ala + ATP	-	Escherichia coli	D-Ala-D-Ala + ADP + phosphate	-	?	411923	
6.1.2.1	D-Ala + D-Ala + ATP	-	Enterococcus faecalis	D-Ala-D-Ala + ADP + phosphate	-	?	411923	
6.1.2.1	D-Ala + D-Ala + ATP	-	Enterococcus faecium	D-Ala-D-Ala + ADP + phosphate	-	?	411923	
6.1.2.1	D-Ala + D-Ala + ATP	-	Enterococcus faecium BM4147	D-Ala-D-Ala + ADP + phosphate	-	?	411923	
6.1.2.1	D-Ala + D-lactate + ATP	-	Escherichia coli	D-Ala-D-lactate + ADP + phosphate	-	?	411924	
6.1.2.1	D-Ala + D-lactate + ATP	-	Enterococcus faecalis	D-Ala-D-lactate + ADP + phosphate	-	?	411924	
6.1.2.1	D-Ala + D-lactate + ATP	-	Enterococcus faecium	D-Ala-D-lactate + ADP + phosphate	-	?	411924	
6.1.2.1	D-Ala + D-lactate + ATP	-	Enterococcus faecium BM4147	D-Ala-D-lactate + ADP + phosphate	-	?	411924	
6.1.2.1	D-alanine + (R)-lactate + ATP	-	Pseudomonas fluorescens	D-alanyl-(R)-lactate + ADP + phosphate	-	?	416811	
6.1.2.1	D-alanine + (R)-lactate + ATP	-	Enterococcus faecalis	D-alanyl-(R)-lactate + ADP + phosphate	-	?	416811	
6.1.2.1	D-alanine + (R)-lactate + ATP	-	Enterococcus faecium	D-alanyl-(R)-lactate + ADP + phosphate	-	?	416811	
6.1.2.1	D-alanine + (R)-lactate + ATP	-	Leuconostoc mesenteroides	D-alanyl-(R)-lactate + ADP + phosphate	-	?	416811	
6.1.2.1	D-alanine + (R)-lactate + ATP	-	Streptomyces coelicolor	D-alanyl-(R)-lactate + ADP + phosphate	-	?	416811	
6.1.2.1	D-alanine + (R)-lactate + ATP	-	Comamonas testosteroni	D-alanyl-(R)-lactate + ADP + phosphate	-	?	416811	
6.1.2.1	D-alanine + (R)-lactate + ATP	-	Raoultella planticola	D-alanyl-(R)-lactate + ADP + phosphate	-	?	416811	
6.1.2.1	D-alanine + (R)-lactate + ATP	-	Desulfitobacterium hafniense	D-alanyl-(R)-lactate + ADP + phosphate	-	?	416811	
6.1.2.1	D-alanine + (R)-lactate + ATP	-	Pseudomonas plecoglossicida	D-alanyl-(R)-lactate + ADP + phosphate	-	?	416811	
6.1.2.1	D-alanine + (R)-lactate + ATP	-	Pseudomonas koreensis	D-alanyl-(R)-lactate + ADP + phosphate	-	?	416811	
6.1.2.1	D-alanine + (R)-lactate + ATP	mutants variants Y207F, S137G/Y207F, S137F/Y207F, S137T/Y207F, and S137A/Y207F from D-alanine-D-alanine ligase, EC 6.3.2.4. The wild-type D-alanine-D-alanine ligase, EC 6.3.2.4 does not show activity with (R)-lactate	Thermotoga maritima	D-alanyl-(R)-lactate + ADP + phosphate	-	?	416811	
6.1.2.1	D-alanine + (R)-lactate + ATP	-	Enterococcus faecalis Cr07	D-alanyl-(R)-lactate + ADP + phosphate	-	?	416811	
6.1.2.1	D-alanine + (R)-lactate + ATP	-	Enterococcus faecium BM4147	D-alanyl-(R)-lactate + ADP + phosphate	-	?	416811	
6.1.2.1	D-alanine + (R)-lactate + ATP	-	Comamonas testosteroni Ni11	D-alanyl-(R)-lactate + ADP + phosphate	-	?	416811	
6.1.2.1	D-alanine + (R)-lactate + ATP	-	Desulfitobacterium hafniense Y51	D-alanyl-(R)-lactate + ADP + phosphate	-	?	416811	
6.1.2.1	D-alanine + (R)-lactate + ATP	-	Streptomyces coelicolor ATCC BAA-471	D-alanyl-(R)-lactate + ADP + phosphate	-	?	416811	
6.1.2.1	D-alanine + (R)-lactate + ATP	-	Enterococcus faecium 330	D-alanyl-(R)-lactate + ADP + phosphate	-	?	416811	
6.1.2.1	D-alanine + (R)-lactate + ATP	-	Pseudomonas plecoglossicida Ag10	D-alanyl-(R)-lactate + ADP + phosphate	-	?	416811	
6.1.2.1	D-alanine + (R)-lactate + ATP	-	Pseudomonas koreensis Hg11	D-alanyl-(R)-lactate + ADP + phosphate	-	?	416811	
6.1.2.1	D-alanine + (R)-lactate + ATP	-	Pseudomonas koreensis Hg10	D-alanyl-(R)-lactate + ADP + phosphate	-	?	416811	
6.1.2.1	D-alanine + (R)-lactate + ATP	-	Pseudomonas fluorescens SDS3	D-alanyl-(R)-lactate + ADP + phosphate	-	?	416811	
6.1.2.1	D-alanine + (R)-lactate + ATP	mutants variants Y207F, S137G/Y207F, S137F/Y207F, S137T/Y207F, and S137A/Y207F from D-alanine-D-alanine ligase, EC 6.3.2.4. The wild-type D-alanine-D-alanine ligase, EC 6.3.2.4 does not show activity with (R)-lactate	Thermotoga maritima ATCC 43589	D-alanyl-(R)-lactate + ADP + phosphate	-	?	416811	
6.1.2.1	D-alanine + (R)-lactate + ATP	-	Enterococcus faecalis Pb06	D-alanyl-(R)-lactate + ADP + phosphate	-	?	416811	
6.1.2.1	D-alanine + (R)-lactate + ATP	-	Enterococcus faecalis E07	D-alanyl-(R)-lactate + ADP + phosphate	-	?	416811	
6.1.2.1	D-alanine + (R)-lactate + ATP	-	Raoultella planticola Ag11	D-alanyl-(R)-lactate + ADP + phosphate	-	?	416811	
6.1.2.1	D-alanine + (R)-lactate + ATP	-	Streptomyces coelicolor M145	D-alanyl-(R)-lactate + ADP + phosphate	-	?	416811	
6.1.2.1	D-alanine + (R)-lactate + ATP	-	Pseudomonas koreensis Cu12	D-alanyl-(R)-lactate + ADP + phosphate	-	?	416811	
6.1.2.1	additional information	reaction proceeds via rapid and reversible formation of the enzyme intermediate D-Ala-phosphate. Reversible cleavage of ATP to ADP and D-Ala-phosphate and reformation of ATP are detectable. D-Ala-phosphate is a common intermediate that serves as the electrophilic substrate in the condensation with D-Lac as nucleophilic cosubstrate	Enterococcus faecium	?	-	?	89	
6.1.2.1	additional information	D-alanine:D-alanine (D-lactate) ligase (ADP) from Leuconostoc mesenteroides synthesizes the depsipeptide, D-alanyl-D-lactate, in addition to D-alanyl-D-alanine, when D-alanine and D-lactate are incubated simultaneously. Structure of bound D-alanine and D-lactate at the active subsites and substrate orientations, overview. With D-lactate a bifurcated H-bond from Arg301 to the R-OH of D-lactate may account for its orientation and nucleophile activation. This orientation is observed when the guanidino side chain of this residue is flexible. D-Alanine adopts an orientation that utilizes H-bonding to water 2882 and the D-alanyl phosphate in subsite 1. Both of these orientations provide mechanisms of deprotonation and place the nucleophile within 3.2 A of the electrophilic carbonyl of the D-alanyl phosphate intermediate for formation of the transition state, molecular docking and chiral specificity of lactate and alanine dockings, detailed overview	Leuconostoc mesenteroides	?	-	?	89	
6.1.2.1	additional information	ligand binding sites of VRSA-9 Ddl, overview	Staphylococcus aureus	?	-	?	89	
6.1.2.1	additional information	substrate specificity of recombinant D-alanine-D-alanine ligase mutant S137G/Y207F, overview	Thermotoga maritima	?	-	?	89	
6.1.2.1	additional information	ligand binding sites of VRSA-9 Ddl, overview	Staphylococcus aureus VRSA-9	?	-	?	89	
6.1.2.1	additional information	substrate specificity of recombinant D-alanine-D-alanine ligase mutant S137G/Y207F, overview	Thermotoga maritima ATCC 43589	?	-	?	89