5.4.99.20	23S rRNA uridine2457	-	Escherichia coli	23S rRNA pseudouridine2457	-	?	407019	
5.4.99.20	23S rRNA uridine2457	-	Escherichia coli K12	23S rRNA pseudouridine2457	-	?	407019	
5.4.99.20	23S rRNA uridine2457	Escherichia coli pseudouridine synthase RluE targeting a single uridine in helix 89 (H89) in the peptidyltransferase center (PTC) of 23S rRNA. The target of RluE in H89 is Psi2457, analysis, overview	Escherichia coli	23S rRNA pseudouridine2457	-	?	407019	
5.4.99.20	23S rRNA uridine2457	Escherichia coli pseudouridine synthase RluE targeting a single uridine in helix 89 (H89) in the peptidyltransferase center (PTC) of 23S rRNA. The target of RluE in H89 is Psi2457, analysis, overview	Escherichia coli K12	23S rRNA pseudouridine2457	-	?	407019	
5.4.99.20	23S rRNA uridine2457	pseudouridine synthase RluE modifies U2457 in a stem of 23S RNA in Escherichia coli. This modification is located in the peptidyl transferase center of the ribosome. The stem alone is not a good RluE substrate, suggesting RluE undergoes additional interactions with other regions in the ribosome	Escherichia coli	23S rRNA pseudouridine2457	-	?	407019	
5.4.99.20	23S rRNA uridine2457	pseudouridine synthase RluE modifies U2457 in a stem of 23S RNA in Escherichia coli. This modification is located in the peptidyl transferase center of the ribosome.The stem alone is not a good RluE substrate, suggesting RluE undergoes additional interactions with other regions in the ribosome	Escherichia coli	23S rRNA pseudouridine2457	-	?	407019	
5.4.99.20	additional information	RluE acts as a stand-alone, highly specific enzyme forming the universally conserved pseudouridine at position 2457, located in helix 89 (H89) of the 23S rRNA in the peptidyltransferase center, detailed structure-function analysis to determine the structural elements both in RluE and in 23S rRNA required for RNA-protein interaction and pseudouridine formation	Escherichia coli	?	-	?	89	
5.4.99.20	additional information	RluE acts as a stand-alone, highly specific enzyme forming the universally conserved pseudouridine at position 2457, located in helix 89 (H89) of the 23S rRNA in the peptidyltransferase center, detailed structure-function analysis to determine the structural elements both in RluE and in 23S rRNA required for RNA-protein interaction and pseudouridine formation	Escherichia coli K12	?	-	?	89	
5.4.99.20	additional information	RluE recognizes a large part of 23S rRNA comprising both H89 and the single-stranded flanking regions which explains the high substrate specificity of RluE. Within RluE, the target RNA is recognized through sequence-specific contacts with loop L7-8 as well as interactions with loop L1-2 and the flexible N-terminal region	Escherichia coli	?	-	?	89	
5.4.99.20	additional information	RluE recognizes a large part of 23S rRNA comprising both H89 and the single-stranded flanking regions which explains the high substrate specificity of RluE. Within RluE, the target RNA is recognized through sequence-specific contacts with loop L7-8 as well as interactions with loop L1-2 and the flexible N-terminal region	Escherichia coli K12	?	-	?	89	
5.4.99.20	23S rRNA uridine2457	the enzyme has an essential Asp at postion 79	Escherichia coli	23S rRNA pseudouridine2457	-	?	407019