4.2.2.16 ascorbic acid + levan transfructosylation. When 35% of ascorbic acid and 2% of levan are incubated with LFTase of 0.5 unit/g levan at 37°C for 85 h, a maximum 52 g/l of ascorbic acid 2-fructoside is produced Paenarthrobacter ureafaciens ascorbic acid 2-fructoside + ? - ? 391319 4.2.2.16 levan - Paenarthrobacter ureafaciens 5 di-D-fructose-2,6':2',6 dianhydride + ? - ? 368348 4.2.2.16 levan - Pseudomonas chlororaphis subsp. aurantiaca 5 di-D-fructose-2,6':2',6 dianhydride + ? - ? 368348 4.2.2.16 levan - Microbacterium sp. 5 di-D-fructose-2,6':2',6 dianhydride + ? - ? 368348 4.2.2.16 levan - Paenarthrobacter nicotinovorans 5 di-D-fructose-2,6':2',6 dianhydride + ? - ? 368348 4.2.2.16 levan - Paenarthrobacter nicotinovorans 5 di-D-fructose-2,6':2',6 dianhydride + ? di-D-fructose-2,6':2',6 dianhydride is the main product, 75%. Fructose, levanbiose, and two unidentified oligosaccharides are minor products ? 368348 4.2.2.16 levan levan from Zymomonas mobilis, conversion yield is 35% Pseudarthrobacter oxydans 5 di-D-fructose-2,6':2',6 dianhydride + ? - ? 368348 4.2.2.16 levan levan from Zymomonas mobilis: conversion yield 28.4% after 24 h. Levan from Serratia: conversion yield: 28.4% after 24 h Microbacterium sp. 5 di-D-fructose-2,6':2',6 dianhydride + ? - ? 368348 4.2.2.16 levan the enzyme degrades Bacillus mesentericus levan up to 40% leaving the levan fragment at the limit of the enzymatic degradation, formation of 5% reducing sugars as D-fructose Paenarthrobacter ureafaciens 5 di-D-fructose-2,6':2',6 dianhydride + ? and two or three minor fructooligosaccharides ? 368348 4.2.2.16 levan the enzyme is specifically induced by levan Paenarthrobacter nicotinovorans 5 di-D-fructose-2,6':2',6 dianhydride + ? - ? 368348 4.2.2.16 levan - Paenarthrobacter ureafaciens K2032 5 di-D-fructose-2,6':2',6 dianhydride + ? - ? 368348 4.2.2.16 levan - Paenarthrobacter nicotinovorans GS-9 5 di-D-fructose-2,6':2',6 dianhydride + ? di-D-fructose-2,6':2',6 dianhydride is the main product, 75%. Fructose, levanbiose, and two unidentified oligosaccharides are minor products ? 368348 4.2.2.16 levan the enzyme is specifically induced by levan Paenarthrobacter nicotinovorans GS-9 5 di-D-fructose-2,6':2',6 dianhydride + ? - ? 368348 4.2.2.16 levan - Paenarthrobacter nicotinovorans GS-9 5 di-D-fructose-2,6':2',6 dianhydride + ? - ? 368348 4.2.2.16 levan - Paenarthrobacter nicotinovorans GS9 5 di-D-fructose-2,6':2',6 dianhydride + ? - ? 368348 4.2.2.16 levan - Pseudomonas chlororaphis subsp. aurantiaca S-4380 5 di-D-fructose-2,6':2',6 dianhydride + ? - ? 368348 4.2.2.16 levan levan from Zymomonas mobilis, conversion yield is 35% Pseudarthrobacter oxydans J17-21 5 di-D-fructose-2,6':2',6 dianhydride + ? - ? 368348 4.2.2.16 levan levan from Zymomonas mobilis: conversion yield 28.4% after 24 h. Levan from Serratia: conversion yield: 28.4% after 24 h Microbacterium sp. AL-210 5 di-D-fructose-2,6':2',6 dianhydride + ? - ? 368348 4.2.2.16 levan - Microbacterium sp. AL-210 5 di-D-fructose-2,6':2',6 dianhydride + ? - ? 368348 4.2.2.16 levan - Paenarthrobacter ureafaciens di-D-fructofuranose-2,6':2',6 dianhydride + ? and small amounts of several oligosaccharides and free fructose ? 368350 4.2.2.16 levan - Paenarthrobacter ureafaciens K2032 di-D-fructofuranose-2,6':2',6 dianhydride + ? and small amounts of several oligosaccharides and free fructose ? 368350 4.2.2.16 levan - Paenarthrobacter ureafaciens di-D-fructose-2,6':2',6 dianhydride + fructose + limited levan di-D-fructose-2,6':2',6 dianhydride (49.1%) + fructose (3.5%) + limited levan (16.2%) ? 382214 4.2.2.16 levan - Paenarthrobacter ureafaciens K2032 di-D-fructose-2,6':2',6 dianhydride + fructose + limited levan di-D-fructose-2,6':2',6 dianhydride (49.1%) + fructose (3.5%) + limited levan (16.2%) ? 382214 4.2.2.16 additional information no activity with dextran, soluble starch, inulin, maltose, sucrose, raffinose, 1-kestose, nystose and 1F-fructofuranosyl nystose Microbacterium sp. ? - ? 89 4.2.2.16 additional information no activity with inulin, levanbiose, sucrose, dextran or starch Pseudarthrobacter oxydans ? - ? 89 4.2.2.16 additional information no formation of fructose, levanbiose, nor difructose anhydride IV from bifurcose and [O-beta-D-Fru(f)-(2-1)]-O-[beta-D-Fru(f)-(2-6)]2-O-beta-D-Fru(f)-(2-1)-alpha-D-G(p) Paenarthrobacter ureafaciens ? - ? 89 4.2.2.16 additional information the enzyme never hydrolyzes beta-2,1-fructosyl-linked fructans, such as inulin, as well as oligosaccharides such as 1-kestose and nystose Microbacterium sp. ? - ? 89 4.2.2.16 additional information no activity with inulin, levanbiose, sucrose, dextran or starch Pseudarthrobacter oxydans J17-21 ? - ? 89 4.2.2.16 additional information no activity with dextran, soluble starch, inulin, maltose, sucrose, raffinose, 1-kestose, nystose and 1F-fructofuranosyl nystose Microbacterium sp. AL-210 ? - ? 89 4.2.2.16 additional information the enzyme never hydrolyzes beta-2,1-fructosyl-linked fructans, such as inulin, as well as oligosaccharides such as 1-kestose and nystose Microbacterium sp. AL-210 ? - ? 89 4.2.2.16 phlein while it degrades Phleum pratense phlein more than 90%, formation of 6% reducing sugars as D-fructose Paenarthrobacter ureafaciens di-D-fructose-2,6':2',6 dianhydride and two or three minor fructooligosaccharides ? 368349 4.2.2.16 raffinose + D-galactopyranose transfer of the beta-fructofuranosyl residue from donor substrate to the 1-C of the acceptor galactopyranose, equilibrium towards galactosyl-fructoside Bacillus subtilis ? - r 382704 4.2.2.16 raffinose + D-galactopyranose transfer of the beta-fructofuranosyl residue from donor substrate to the 1-C of the acceptor galactopyranose, equilibrium towards galactosyl-fructoside Bacillus subtilis 168 ? - r 382704 4.2.2.16 stachyose + D-galactopyranose transfer of the beta-fructofuranosyl residue from donor substrate to the 1-C of the acceptor galactopyranose, equilibrium towards galactosyl-fructoside Bacillus subtilis ? - r 382828 4.2.2.16 stachyose + D-galactopyranose transfer of the beta-fructofuranosyl residue from donor substrate to the 1-C of the acceptor galactopyranose, equilibrium towards galactosyl-fructoside Bacillus subtilis 168 ? - r 382828 4.2.2.16 sucrose + D-galactopyranose transfer of the beta-fructofuranosyl residue from donor substrate to the 1-C of the acceptor galactopyranose, equilibrium towards galactosyl-fructoside Bacillus subtilis ? - r 382849 4.2.2.16 sucrose + D-galactopyranose transfer of the beta-fructofuranosyl residue from donor substrate to the 1-C of the acceptor galactopyranose, equilibrium towards galactosyl-fructoside Bacillus subtilis 168 ? - r 382849