3.6.1.1 2-phosphorylethanolamine + H2O low activity Arabidopsis thaliana ethanolamine + phosphate - ? 419936 3.6.1.1 3'-AMP + H2O 4% activity compared to diphosphate Thermococcus onnurineus adenosine + phosphate - ? 64617 3.6.1.1 4-nitrophenyl phosphate + H2O low activity Arabidopsis thaliana 4-nitrophenol + phosphate - ? 73771 3.6.1.1 4-nitrophenyl phosphate + H2O low activity Arabidopsis thaliana Col-0 4-nitrophenol + phosphate - ? 73771 3.6.1.1 ADP + H2O - Pyrococcus furiosus ? - ? 64621 3.6.1.1 ADP + H2O 2.7% relative activity compared to diphosphate Rhodospirillum rubrum ? - ? 64621 3.6.1.1 ADP + H2O 2.6% relative activity compared to diphosphate Acidithiobacillus thiooxidans ? - ? 64621 3.6.1.1 ADP + H2O 18% relative activity compared to diphosphate Kitasatospora aureofaciens ? - ? 64621 3.6.1.1 ADP + H2O hydrolyzed at 36.2% of the rate compared to diphosphate Sulfolobus acidocaldarius ? - ? 64621 3.6.1.1 ADP + H2O hydrolyzed at 36.2% of the rate compared to diphosphate Sulfolobus acidocaldarius 7 ? - ? 64621 3.6.1.1 ADP + H2O - Pyrococcus furiosus ATCC 43587 ? - ? 64621 3.6.1.1 ADP + H2O 18% relative activity compared to diphosphate Kitasatospora aureofaciens RIA 57 ? - ? 64621 3.6.1.1 ADP + H2O 2.7% relative activity compared to diphosphate Rhodospirillum rubrum G-9 ? - ? 64621 3.6.1.1 ADP + H2O 18% relative activity compared to diphosphate Kitasatospora aureofaciens RIA57 ? - ? 64621 3.6.1.1 ADP + H2O 5% activity compared to diphosphate Thermococcus onnurineus AMP + phosphate - ? 93189 3.6.1.1 ADP + H2O 3-6% of the activity with diphosphate Sulfolobus acidocaldarius ? + phosphate - ? 391192 3.6.1.1 AMP + H2O 3.6% relative activity compared to diphosphate Kitasatospora aureofaciens ? - ? 141188 3.6.1.1 ATP + H2O - Pyrococcus furiosus ? - ? 64622 3.6.1.1 ATP + H2O - Plasmodium falciparum ? - ? 64622 3.6.1.1 ATP + H2O hydrolysis only with Zn2+ or Mn2+ as cofactors Saccharomyces cerevisiae ? - ? 64622 3.6.1.1 ATP + H2O 7.8% relative activity compared to diphosphate Kitasatospora aureofaciens ? - ? 64622 3.6.1.1 ATP + H2O 1.3% relative activity compared to diphosphate Rhodospirillum rubrum ? - ? 64622 3.6.1.1 ATP + H2O 4.2% relative activity compared to diphosphate Acidithiobacillus thiooxidans ? - ? 64622 3.6.1.1 ATP + H2O 3% relative activity compared to diphosphate Capsicum annuum ? - ? 64622 3.6.1.1 ATP + H2O reaction only in the presence of Mn2+ Escherichia coli ? - ? 64622 3.6.1.1 ATP + H2O reaction only in the presence of Mn2+ Rattus norvegicus ? - ? 64622 3.6.1.1 ATP + H2O reaction only in the presence of Mn2+ or Zn2+ Saccharomyces cerevisiae ? - ? 64622 3.6.1.1 ATP + H2O hydrolyzed at 19.4% of the rate compared to diphosphate Sulfolobus acidocaldarius ? - ? 64622 3.6.1.1 ATP + H2O hydrolyzed at 19.4% of the rate compared to diphosphate Sulfolobus acidocaldarius 7 ? - ? 64622 3.6.1.1 ATP + H2O - Pyrococcus furiosus ATCC 43587 ? - ? 64622 3.6.1.1 ATP + H2O reaction only in the presence of Mn2+ Escherichia coli MRE 600 ? - ? 64622 3.6.1.1 ATP + H2O 7.8% relative activity compared to diphosphate Kitasatospora aureofaciens RIA 57 ? - ? 64622 3.6.1.1 ATP + H2O 1.3% relative activity compared to diphosphate Rhodospirillum rubrum G-9 ? - ? 64622 3.6.1.1 ATP + H2O 7.8% relative activity compared to diphosphate Kitasatospora aureofaciens RIA57 ? - ? 64622 3.6.1.1 ATP + H2O 1% activity compared to diphosphate Thermococcus onnurineus ADP + phosphate - ? 92967 3.6.1.1 ATP + H2O 4% activity compared to diphosphate Thermococcus onnurineus ADP + phosphate - ? 92967 3.6.1.1 ATP + H2O 3-6% of the activity with diphosphate Sulfolobus acidocaldarius ? + phosphate - ? 391344 3.6.1.1 c-Jun N-terminale kinase + H2O - Gallus gallus ? - ? 432719 3.6.1.1 CDP + H2O - Pyrococcus furiosus ? - ? 64641 3.6.1.1 CDP + H2O - Pyrococcus furiosus ATCC 43587 ? - ? 64641 3.6.1.1 CTP + H2O - Pyrococcus furiosus ? - ? 64642 3.6.1.1 CTP + H2O - Pyrococcus furiosus ATCC 43587 ? - ? 64642 3.6.1.1 cyclic tripolyphosphate + H2O hydrolyzed at 32.3% of the rate compared to diphosphate Sulfolobus acidocaldarius ? - ? 420509 3.6.1.1 cyclic tripolyphosphate + H2O hydrolyzed at 32.3% of the rate compared to diphosphate Sulfolobus acidocaldarius 7 ? - ? 420509 3.6.1.1 D-fructose-6-phosphate + H2O 13% relative activity compared to diphosphate Kitasatospora aureofaciens D-fructose + phosphate - ? 141191 3.6.1.1 D-glucose-1-phosphate + H2O very weak substrate compared to diphosphate Sorghum bicolor D-glucose + phosphate - ? 141182 3.6.1.1 D-glucose-1-phosphate + H2O 15% relative activity compared to diphosphate Kitasatospora aureofaciens D-glucose + phosphate - ? 141182 3.6.1.1 D-glucose-6-phosphate + H2O low activity Arabidopsis thaliana D-glucose + phosphate - ? 400759 3.6.1.1 dATP + H2O 7% activity compared to diphosphate Thermococcus onnurineus dADP + phosphate - ? 260008 3.6.1.1 diphosphate + H2O - Escherichia coli 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O - Escherichia coli 2 phosphate - r 73540 3.6.1.1 diphosphate + H2O - Homo sapiens 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O - Rattus norvegicus 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O - Sus scrofa 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O - Saccharomyces cerevisiae 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O - Saccharomyces cerevisiae 2 phosphate - r 73540 3.6.1.1 diphosphate + H2O - Bos taurus 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O - Oryctolagus cuniculus 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O - Geobacillus stearothermophilus 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O - Cereibacter sphaeroides 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O - Methanothermobacter thermautotrophicus 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O - Spinacia oleracea 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O - Zea mays 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O - Solanum tuberosum 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O - Arabidopsis thaliana 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O - Entamoeba histolytica 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O - Rubrivivax gelatinosus 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O - Rhodopseudomonas palustris 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O - Sulfolobus acidocaldarius 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O - Rhodobacter capsulatus 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O - Enterococcus faecalis 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O - Ascaris suum 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O - Enterococcus faecium 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O - Moorella thermoacetica 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O - Trypanosoma cruzi 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O - Vigna radiata 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O - Helicobacter pylori 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O - Sorghum bicolor 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O - Rhodospirillum rubrum 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O - Gossypium hirsutum 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O - Kitasatospora aureofaciens 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O - Aspergillus oryzae 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O - Desulfovibrio desulfuricans 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O - Methanocaldococcus jannaschii 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O - Catharanthus roseus 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O - Schizophyllum commune 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O - Thermotoga maritima 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O - Vibrio cholerae serotype O1 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O - Streptococcus mutans 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O - Streptococcus salivarius 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O - Dunaliella salina 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O - Pyrococcus horikoshii 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O - Acidithiobacillus thiooxidans 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O - Saccharum officinarum 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O - Hordeum vulgare subsp. vulgare 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O - Mus sp. 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O - Aranda Christine 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O - Amaranthus blitum 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O - Amaranthus tricolor 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O - Shewanella sp. 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O - Blastochloris viridis 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O - Toxoplasma gondii 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O - Aquifex aeolicus 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O - Papaver rhoeas 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O - Vitis vinifera 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O - Chlamydomonas reinhardtii 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O - Arabidopsis thaliana 2 phosphate - ir 73540 3.6.1.1 diphosphate + H2O - Streptococcus gordonii 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O - Streptococcus gordonii 2 phosphate - r 73540 3.6.1.1 diphosphate + H2O - Synechocystis sp. 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O - Anabaena sp. 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O - Pseudanabaena sp. 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O - Caenorhabditis elegans 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O - Hypophthalmichthys nobilis 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O - Streptococcus agalactiae 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O - Candidatus Korarchaeum cryptofilum 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O - Bacillus subtilis 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O - Bacillus subtilis 2 phosphate - r 73540 3.6.1.1 diphosphate + H2O - Leishmania major 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O - Mycobacterium tuberculosis 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O - Mycobacterium tuberculosis 2 phosphate - r 73540 3.6.1.1 diphosphate + H2O - Sulfurisphaera tokodaii 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O - Methanothrix thermoacetophila 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O - Thermococcus thioreducens 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O - Mus musculus 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O - Staphylococcus aureus 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O - Malus domestica 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O - Plasmodium falciparum 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O - Staphylococcus aureus 2 phosphate - r 73540 3.6.1.1 diphosphate + H2O - Clostridium perfringens 2 phosphate - r 73540 3.6.1.1 diphosphate + H2O - Desulfitobacterium hafniense 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O - Desulfitobacterium hafniense 2 phosphate - r 73540 3.6.1.1 diphosphate + H2O - Streptococcus agalactiae 2 phosphate - r 73540 3.6.1.1 diphosphate + H2O - Papaver rhoeas 2 phosphate - r 73540 3.6.1.1 diphosphate + H2O - Ethanoligenens harbinense 2 phosphate - r 73540 3.6.1.1 diphosphate + H2O - Haloferax volcanii 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O - Tribolium castaneum 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O - Medicago truncatula 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O - Rhipicephalus microplus 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O specific for Pyrococcus horikoshii 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O most specific substrate Pyrococcus furiosus 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O high specificity with Mg2+ as cofactor Saccharomyces cerevisiae 2 phosphate - r 73540 3.6.1.1 diphosphate + H2O reaction may be reversed by coupling to glucose-6-phosphate to 6-phospho-gluconate Escherichia coli 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O reaction may be reversed by coupling to glucose-6-phosphate to 6-phospho-gluconate Escherichia coli 2 phosphate - r 73540 3.6.1.1 diphosphate + H2O no other substrates Geobacillus stearothermophilus 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O actual substrate is magnesium diphosphate or dimagnesium diphosphate Bacillus subtilis 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O actual substrate is magnesium diphosphate or dimagnesium diphosphate Escherichia coli 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O actual substrate is magnesium diphosphate or dimagnesium diphosphate Spinacia oleracea 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O actual substrate is magnesium diphosphate or dimagnesium diphosphate Rhodopseudomonas palustris 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O actual substrate is magnesium diphosphate or dimagnesium diphosphate Sulfolobus acidocaldarius 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O actual substrate is magnesium diphosphate or dimagnesium diphosphate Helicobacter pylori 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O actual substrate is magnesium diphosphate or dimagnesium diphosphate Capsicum annuum 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O actual substrate is magnesium diphosphate or dimagnesium diphosphate Cricetinae 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O high specificity Bacillus subtilis 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O high specificity Methanothermobacter thermautotrophicus 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O high specificity Spinacia oleracea 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O high specificity Sulfolobus acidocaldarius 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O high specificity Helicobacter pylori 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O high specificity Sorghum bicolor 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O high specificity Methanocaldococcus jannaschii 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O high specificity Dunaliella salina 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O high specificity Saccharum officinarum 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O anabolism Solanum tuberosum 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O important for energy metabolism, provides energy for biosynthetic reactions Escherichia coli 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O important for energy metabolism, provides energy for biosynthetic reactions Rattus norvegicus 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O important for energy metabolism, provides energy for biosynthetic reactions Saccharomyces cerevisiae 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O important for energy metabolism, provides energy for biosynthetic reactions Streptococcus mutans 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O important for energy metabolism, provides energy for biosynthetic reactions Aquifex aeolicus 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O important for energy metabolism, provides energy for biosynthetic reactions Leishmania major 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O important for energy metabolism, provides energy for biosynthetic reactions, central enzyme of phosphorus metabolism Saccharomyces cerevisiae 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O the enzyme plays an essential role in the worms molting and development, and in larval survival in the host, overview Ascaris suum 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O MgPPi Escherichia coli 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O an essential and ubiquitous metal-dependent enzyme Blastochloris viridis 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O an essential and ubiquitous metal-dependent enzyme Synechocystis sp. 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O an essential and ubiquitous metal-dependent enzyme Anabaena sp. 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O an essential and ubiquitous metal-dependent enzyme Pseudanabaena sp. 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O diphosphate hydrolysis provides a thermodynamic driving force for important biosynthetic reactions, PYP-1 is required for larval development and intestinal function, overview Caenorhabditis elegans 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O intracellular diphosphate is a by-product of multiple biosynthetic reactions and its hydrolysis by cytosolic iPPase is an important homeostatic mechanism favoring biosynthesis, overview Rattus norvegicus 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O LHPPase is associated with hyperthyroidism Homo sapiens 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O PPase plays an essential role in energy conservation and provides the energy for many biosynthetic pathways controlling the intracellular diphosphate levels Helicobacter pylori 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O the enzyme is regulated by reversible phosphorylation, another mechanism in regulation of several physiological processes, e.g. self-incompatibility-mediated pollen tube inhibition, overview Papaver rhoeas 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O diphosphate binding does not cause a significant conformational change in the diphosphate-PPase complex, structure, overview Helicobacter pylori 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O high activity with magnesium diphosphate, low activity with lanthanum diphosphate Escherichia coli 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O Lys112 is supposed to play a key role in forming contacts with the phosphate groups of the three studied effectors, overview Escherichia coli 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O residues Arg43, Lys148, and Lys115 are involved in binding of diphosphate Escherichia coli 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O the enzyme is very specific for diphosphate as substrate, substrate specificity, overview Gossypium hirsutum 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O the rate-determining step for the forward reaction with Mg2+ is hydrolysis of PPi, the wild-type active site shows a closed comformation with one of the two product phosphates already dissociated, active site residues Tyr93 and Asp115 are important, six-state catalytic mechanism, overview Saccharomyces cerevisiae 2 phosphate in the reverse, net synthesis direction, the rate-determining step is not the condensation of the two phosphate ions but the previous step, which involves isomerization of the enzyme r 73540 3.6.1.1 diphosphate + H2O the rate-limiting step of Mn2+-supported hydrolysis of the phosphoanhydride bond is followed by a fast release of the leaving phosphate from the P1 site, overview Escherichia coli 2 phosphate Mg- or Mn-bound substrate for the synthesis reaction r 73540 3.6.1.1 diphosphate + H2O in vivo function as an inorganic diphosphatase. Substrate discrimination is based, in part, on active site space restrictions imposed by the cap domain, specifically by residues Tyr76 and Glu47 Bacteroides thetaiotaomicron 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O as far as the release of phosphate, is concerned, diphosphate, is the best substrate Sulfolobus acidocaldarius 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O AtPPsPase1 catalyzes the specific cleavage Arabidopsis thaliana 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O development and evaluation of a phosphate-based colorimetric assay to measure progress of PCR using the PPase-coupled enzyme assay, overview Pyrococcus horikoshii 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O structure-function analysis, overview. BT2127 conserves the His23-Lys79 diad, and in both the cap-open and -closed conformations, the Asp13 side chain is in the same conformation, engaged in a hydrogen bond with linker residue Ser15 Bacteroides thetaiotaomicron 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O the pyrophosphatase hydrolyzes the major part of diphosphate that is produced in the acetate activation reaction Methanothrix thermoacetophila 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O pronounced specificity for diphosphate Thermoplasma acidophilum 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O the enzyme shows high specificity for diphosphate but low reactivity to sodium tripolyphosphate and sodium tetrapolyphosphate. ADP and ATP can not serve as substrates Pyrococcus horikoshii 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O no significant proton-translocation activity can be assayed in the total membrane fractions of pTVP-transformed yeast cells. This is probably due to the fact that the activity of the hyperthermophilic enzyme is negligible below 50-55°C. Above 50°C the high passive conductance of yeast membranes to protons makes it impossible to establish a reasonable pH gradient as demonstrated with V-PPases that hydrolyze PPi at these temperatures Thermotoga maritima 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O diphosphate in form of Mg-diphosphate Escherichia coli 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O diphosphate in form of Mg-diphosphate Rhodopseudomonas palustris 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O diphosphate in form of Mg-diphosphate Helicobacter pylori 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O diphosphate in form of Mg-diphosphate Streptococcus salivarius 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O diphosphate in form of Ca-diphosphate Rhodopseudomonas palustris 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O diphosphate in form of Co-diphosphate Rhodopseudomonas palustris 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O diphosphate in form of Mn-diphosphate Rhodopseudomonas palustris 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O the enzyme can also catalyze the reverse reaction of diphosphate synthesis from phosphate, but this activity does not seem physiologically important Streptococcus gordonii 2 phosphate - r 73540 3.6.1.1 diphosphate + H2O the enzyme can also catalyze the reverse reaction of diphosphate synthesis from phosphate, but this activity does not seem physiologically important Bacillus subtilis 2 phosphate - r 73540 3.6.1.1 diphosphate + H2O the enzyme can also catalyze the reverse reaction of diphosphate synthesis from phosphate, but this activity does not seem physiologically important Staphylococcus aureus 2 phosphate - r 73540 3.6.1.1 diphosphate + H2O the enzyme can also catalyze the reverse reaction of diphosphate synthesis from phosphate, but this activity does not seem physiologically important Clostridium perfringens 2 phosphate - r 73540 3.6.1.1 diphosphate + H2O the enzyme can also catalyze the reverse reaction of diphosphate synthesis from phosphate, but this activity does not seem physiologically important Desulfitobacterium hafniense 2 phosphate - r 73540 3.6.1.1 diphosphate + H2O the enzyme can also catalyze the reverse reaction of diphosphate synthesis from phosphate, but this activity does not seem physiologically important Streptococcus agalactiae 2 phosphate - r 73540 3.6.1.1 diphosphate + H2O the enzyme can also catalyze the reverse reaction of diphosphate synthesis from phosphate, but this activity does not seem physiologically important Papaver rhoeas 2 phosphate - r 73540 3.6.1.1 diphosphate + H2O - Methanothermobacter thermautotrophicus H 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O high specificity Methanothermobacter thermautotrophicus H 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O - Pyrococcus horikoshii DSM 12428 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O development and evaluation of a phosphate-based colorimetric assay to measure progress of PCR using the PPase-coupled enzyme assay, overview Pyrococcus horikoshii DSM 12428 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O the enzyme shows high specificity for diphosphate but low reactivity to sodium tripolyphosphate and sodium tetrapolyphosphate. ADP and ATP can not serve as substrates Pyrococcus horikoshii DSM 12428 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O - Sulfolobus acidocaldarius DSM 639 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O - Sulfolobus acidocaldarius 7 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O actual substrate is magnesium diphosphate or dimagnesium diphosphate Sulfolobus acidocaldarius 7 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O high specificity Sulfolobus acidocaldarius 7 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O as far as the release of phosphate, is concerned, diphosphate, is the best substrate Sulfolobus acidocaldarius 7 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O - Leishmania major 252 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O important for energy metabolism, provides energy for biosynthetic reactions Leishmania major 252 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O - Streptococcus gordonii DL1 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O - Desulfitobacterium hafniense DSM 10664 2 phosphate - r 73540 3.6.1.1 diphosphate + H2O - Escherichia coli MRE 600 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O important for energy metabolism, provides energy for biosynthetic reactions Escherichia coli MRE 600 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O - Shewanella sp. AS-11 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O - Sulfurisphaera tokodaii 7 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O in vivo function as an inorganic diphosphatase. Substrate discrimination is based, in part, on active site space restrictions imposed by the cap domain, specifically by residues Tyr76 and Glu47 Bacteroides thetaiotaomicron ATCC 29148 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O structure-function analysis, overview. BT2127 conserves the His23-Lys79 diad, and in both the cap-open and -closed conformations, the Asp13 side chain is in the same conformation, engaged in a hydrogen bond with linker residue Ser15 Bacteroides thetaiotaomicron ATCC 29148 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O - Thermotoga maritima DSM 3109 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O no significant proton-translocation activity can be assayed in the total membrane fractions of pTVP-transformed yeast cells. This is probably due to the fact that the activity of the hyperthermophilic enzyme is negligible below 50-55°C. Above 50°C the high passive conductance of yeast membranes to protons makes it impossible to establish a reasonable pH gradient as demonstrated with V-PPases that hydrolyze PPi at these temperatures Thermotoga maritima DSM 3109 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O - Kitasatospora aureofaciens RIA 57 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O - Arabidopsis thaliana Col-0 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O AtPPsPase1 catalyzes the specific cleavage Arabidopsis thaliana Col-0 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O - Clostridium perfringens type A 2 phosphate - r 73540 3.6.1.1 diphosphate + H2O the enzyme can also catalyze the reverse reaction of diphosphate synthesis from phosphate, but this activity does not seem physiologically important Clostridium perfringens type A 2 phosphate - r 73540 3.6.1.1 diphosphate + H2O - Streptococcus gordonii V288 2 phosphate - r 73540 3.6.1.1 diphosphate + H2O the enzyme can also catalyze the reverse reaction of diphosphate synthesis from phosphate, but this activity does not seem physiologically important Streptococcus gordonii V288 2 phosphate - r 73540 3.6.1.1 diphosphate + H2O - Desulfitobacterium hafniense DCB-2 2 phosphate - r 73540 3.6.1.1 diphosphate + H2O - Clostridium perfringens 13 2 phosphate - r 73540 3.6.1.1 diphosphate + H2O the enzyme can also catalyze the reverse reaction of diphosphate synthesis from phosphate, but this activity does not seem physiologically important Clostridium perfringens 13 2 phosphate - r 73540 3.6.1.1 diphosphate + H2O - Rhodospirillum rubrum G-9 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O - Saccharomyces cerevisiae ATCC 204508 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O - Mycobacterium tuberculosis H37Rv 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O - Ethanoligenens harbinense UniProt 2 phosphate - r 73540 3.6.1.1 diphosphate + H2O - Escherichia coli MG1655 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O - Rhodopseudomonas palustris Dr2 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O - Rubrivivax gelatinosus Dr2 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O - Staphylococcus aureus Mu50 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O - Pyrococcus horikoshii OT-3 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O specific for Pyrococcus horikoshii OT-3 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O - Kitasatospora aureofaciens RIA57 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O - Enterococcus faecium F24 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O - Methanothrix thermoacetophila DSM 6194 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O the pyrophosphatase hydrolyzes the major part of diphosphate that is produced in the acetate activation reaction Methanothrix thermoacetophila DSM 6194 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O actual substrate is magnesium diphosphate or dimagnesium diphosphate Helicobacter pylori 8823 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O high specificity Helicobacter pylori 8823 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O diphosphate in form of Mg-diphosphate Helicobacter pylori 8823 2 phosphate - ? 73540 3.6.1.1 diphosphate + H2O - Saccharomyces cerevisiae phosphate + phosphate - ? 414558 3.6.1.1 diphosphate + H2O - Vibrio cholerae serotype O1 phosphate + phosphate - ? 414558 3.6.1.1 diphosphate + H2O - Trypanosoma cruzi phosphate + phosphate - ? 414558 3.6.1.1 diphosphate + H2O - Mycoplasma suis phosphate + phosphate - ? 414558 3.6.1.1 diphosphate + H2O - Thermus thermophilus phosphate + phosphate - ? 414558 3.6.1.1 diphosphate + H2O 100% activity Thermococcus onnurineus phosphate + phosphate - ? 414558 3.6.1.1 diphosphate + H2O - Trypanosoma cruzi Y phosphate + phosphate - ? 414558 3.6.1.1 diphosphate + H2O - Thermus thermophilus HB8 / ATCC 27634 / DSM 579 phosphate + phosphate - ? 414558 3.6.1.1 diphosphates + H2O hydrolysis of organic diphosphates in the presence of Zn2+ Saccharomyces cerevisiae ? - ? 141183 3.6.1.1 GDP + H2O - Pyrococcus furiosus ? - ? 73835 3.6.1.1 GDP + H2O 20% relative activity compared to diphosphate Kitasatospora aureofaciens ? - ? 73835 3.6.1.1 GMP + H2O 18% relative activity compared to diphosphate Kitasatospora aureofaciens ? - ? 141189 3.6.1.1 GTP + H2O - Pyrococcus furiosus ? - ? 64639 3.6.1.1 GTP + H2O - Pyrococcus furiosus ATCC 43587 ? - ? 64639 3.6.1.1 guanosine 5'-tetraphosphate + H2O - Trypanosoma cruzi ? - ? 373356 3.6.1.1 IDP + H2O - Pyrococcus furiosus ? - ? 64645 3.6.1.1 imidodiphosphate + 2 H2O hydrolysis even in the absence of divalent cations Bos taurus 2 phosphate + NH3 - ir 141179 3.6.1.1 imidodiphosphate + H2O active site and substrate binding structure determination and analysis, overview Bacillus subtilis phosphate + phosphoramidic acid - ? 397463 3.6.1.1 ITP + H2O - Pyrococcus furiosus ? - ? 64646 3.6.1.1 ITP + H2O 10% of the activity with diphosphate Sulfolobus acidocaldarius ? + phosphate - ? 392296 3.6.1.1 additional information enzyme may be identical with EC 3.1.3.1 or EC 3.1.3.9 Aspergillus oryzae ? - ? 89 3.6.1.1 additional information no hydrolysis of ATP and traces of activity with tripolyphosphate in the presence of Mg2+ Saccharomyces cerevisiae ? - ? 89 3.6.1.1 additional information no hydrolysis of ATP in the presence of Mg2+ Escherichia coli ? - ? 89 3.6.1.1 additional information no hydrolysis of ATP in the presence of Mg2+ Rattus norvegicus ? - ? 89 3.6.1.1 additional information no hydrolysis of ATP in the presence of Mg2+ Streptococcus mutans ? - ? 89 3.6.1.1 additional information polyphosphates and ATP are not hydrolyzed Leishmania major ? - ? 89 3.6.1.1 additional information the enzyme is a V-type vacuolar H+-pump and responsible for phosphate uptake across the vacuolar membrane, mechanism of activation of phosphate uptake into the vacuole under low phosphate status, overview Catharanthus roseus ? - ? 89 3.6.1.1 additional information the enzyme acts as diphosphate-dependent H+-translocation pump Vigna radiata ? - ? 89 3.6.1.1 additional information triphosphate, ATP, and ADP are poor substrates, AMP, phosphoenolpyruvate, and 4-nitrophenyl phosphate are no substrates Pyrococcus horikoshii ? - ? 89 3.6.1.1 additional information enzyme is specific for diphosphate and does not cleave nucleotide-polyphosphates Sulfolobus acidocaldarius ? - ? 89 3.6.1.1 additional information cytosolic phosphoproteins p26.1 from incompatible pollen show rapid, self-incompatibility-induced Ca2+-dependent hyperphosphorylation in vivo, overview Papaver rhoeas ? - ? 89 3.6.1.1 additional information active site residues are His9, Arg13, Asp15, Asp77, His99, His100, Asp151, Lys207, Arg297 and Lys298 Streptococcus agalactiae ? - ? 89 3.6.1.1 additional information no activity with sodium triphosphate, ADP, ATP, and glucose-6-phosphate Hypophthalmichthys nobilis ? - ? 89 3.6.1.1 additional information the enzyme contains an extra binding site for the substrate magnesium diphosphate or its non-hydrolyzable analogue magnesium methylenediphosphonate, binding of substrate at the effector site of pyrophosphatase increases the rate of its hydrolysis at the active site, overview Escherichia coli ? - ? 89 3.6.1.1 additional information dCTP, ADP, dAMP, phosphoglycolate, phosphoserine, polyphosphate, and 4-nitrophenyl phosphate are no substrates for PPase Thermococcus onnurineus ? - ? 89 3.6.1.1 additional information no activity with PAP, PAPS, 3'-CMP, fructose 1,6-bisphosphate, and D-myo-inositol 1-monophosphate Thermococcus onnurineus ? - ? 89 3.6.1.1 additional information BT2127 substrate specificity profile, overview Bacteroides thetaiotaomicron ? - ? 89 3.6.1.1 additional information substrate specificity, overview. The enzyme is highly specific, no or poor activity with ribose-5-phosphate, phospho-L-serine, o-phosphocholine, phosphoenolpyruvate, L-glycerol-3-phosphate, beta-glycerol-phosphate, and phospho-L-tyrosine Arabidopsis thaliana ? - ? 89 3.6.1.1 additional information the enzyme is possibly involved in glycoprotein biosynthesis Sulfurisphaera tokodaii ? - ? 89 3.6.1.1 additional information the product of the TM0913 gene, has both nucleoside triphosphate pyrophosphohydrolase and pyrophosphatase activities Thermotoga maritima ? - ? 89 3.6.1.1 additional information apple soluble inorganic diphosphatase physically interacts with S-RNases, interaction analysis by pulldown assay with recombinant MBP-tagged S1, S2, S3, S9-RNase and His6-tagged A14 expressed from Escherichia coli, overview Malus domestica ? - - 89 3.6.1.1 additional information the soluble enzyme from cattle tick Rhipicephalus microplus is capable of hydrolysing polyphosphates, molecular docking assays of RmPPase with polyphosphates, and molecular modelling, overview Rhipicephalus microplus ? - - 89 3.6.1.1 additional information a copper(II)-based two-dimensional metal-organic framework with nanosheet structure (CuBDC NS) that possesses peroxidase (POx) mimicking activity is prepared. In the presence of H2O2, the system catalyses the oxidation of terephthalic acid to a blue-fluorescent product (excitation at 315 nm, emission at 425 nm). Diphosphate has a very strong affinity for Cu2+ ion and blocks the POx-mimicking activity of the CuBDC NS. If inorganic diphosphatase is present, the POx mimicking activity is gradually restored because diphosphate is hydrolyzed. Design and evaluation of a method for determination of inorganic pyrophosphatase activity by fluorometry, method, overview Homo sapiens ? - - 89 3.6.1.1 additional information enzyme HvPPA drives thermodynamically unfavorable reactions to completion under conditions of reduced water activity, development of a coupled assay. HvPPA hydrolyzes the PPi by-product generated in 2 M NaCl by UbaA (a salt-loving noncanonical E1 enzyme that adenylates ubiquitin-like proteins in the presence of ATP). Malachite green assay. Significant levels of phosphate are detected when UbaA and HvPPA are coupled with ATP and SAMP1 in the reaction, phosphate is not detected when ATP, UbaA, HvPPA, or SAMP1 are omitted from the adenylation assay, and no or poor amounts of phosphate are generated enzymatically when ATP is replaced by other nucleotides (AMP, ADP, AMP-PNP, CTP, GTP, TTP, and UTP) Haloferax volcanii ? - - 89 3.6.1.1 additional information PfPPase is capable of utilizing PPi, polyP3, and ATP as substrates Plasmodium falciparum ? - - 89 3.6.1.1 additional information the recombinant enzyme rRmPPase has a greater affinity, higher catalytic efficiency and increased cooperativity for sodium phosphate glass type 15 (polyP15) than for sodium tripolyphosphate (polyP3). Molecular docking study. PolyP3 binds close to the Mg2+ atoms in the catalytic region of the protein, participating in their coordination network, whereas polyP15 interactions involve negatively charged phosphate groups and basic amino acid residues, such as Lys56, Arg58, and Lys193. PolyP15 has a more favourable theoretical binding affinity than polyP3, thus supporting the kinetic data Rhipicephalus microplus ? - - 89 3.6.1.1 additional information usage of the malachite green assay. The location and the conformation of the PPi in all 12 active sites are nearly identical, whereas the location and the occupancy of a Ca2+ at position M1 (most commonly coordinating both Asp57 and Asp89) as well as the conformation of the side chain of Asp89 and, to a lesser extent of Asp57, are somewhat variable among the different active sites, substrate binding structures, detailed overview Mycobacterium tuberculosis ? - - 89 3.6.1.1 additional information polyphosphates and ATP are not hydrolyzed Leishmania major 252 ? - ? 89 3.6.1.1 additional information no hydrolysis of ATP in the presence of Mg2+ Escherichia coli MRE 600 ? - ? 89 3.6.1.1 additional information the enzyme is possibly involved in glycoprotein biosynthesis Sulfurisphaera tokodaii 7 ? - ? 89 3.6.1.1 additional information BT2127 substrate specificity profile, overview Bacteroides thetaiotaomicron ATCC 29148 ? - ? 89 3.6.1.1 additional information the product of the TM0913 gene, has both nucleoside triphosphate pyrophosphohydrolase and pyrophosphatase activities Thermotoga maritima DSM 3109 ? - ? 89 3.6.1.1 additional information substrate specificity, overview. The enzyme is highly specific, no or poor activity with ribose-5-phosphate, phospho-L-serine, o-phosphocholine, phosphoenolpyruvate, L-glycerol-3-phosphate, beta-glycerol-phosphate, and phospho-L-tyrosine Arabidopsis thaliana Col-0 ? - ? 89 3.6.1.1 additional information triphosphate, ATP, and ADP are poor substrates, AMP, phosphoenolpyruvate, and 4-nitrophenyl phosphate are no substrates Pyrococcus horikoshii OT-3 ? - ? 89 3.6.1.1 phosphoenolpyruvate + H2O 12% relative activity compared to diphosphate Kitasatospora aureofaciens pyruvate + phosphate - ? 64599 3.6.1.1 phosphoenolpyruvate + H2O 1-2% of the activity with diphosphate Sulfolobus acidocaldarius pyruvate + phosphate - ? 64599 3.6.1.1 phosphoenolpyruvate + H2O 12% relative activity compared to diphosphate Kitasatospora aureofaciens RIA 57 pyruvate + phosphate - ? 64599 3.6.1.1 phosphoenolpyruvate + H2O 12% relative activity compared to diphosphate Kitasatospora aureofaciens RIA57 pyruvate + phosphate - ? 64599 3.6.1.1 polyphosphate + H2O less than 1% activity compared to diphosphate Thermococcus onnurineus ? - ? 364536 3.6.1.1 polyphosphate 75 + H2O - Trypanosoma cruzi ? - ? 434139 3.6.1.1 polyphosphate glass type 15 + H2O - Rhipicephalus microplus ? - ? 454463 3.6.1.1 tetrapolyphosphate + H2O - Escherichia coli ? - ? 141181 3.6.1.1 tetrapolyphosphate + H2O very weak substrate compared to diphosphate Sorghum bicolor ? - ? 141181 3.6.1.1 tetrapolyphosphate + H2O 25.2% activity compared to the activity with diphosphate Pyrococcus horikoshii ? - ? 141181 3.6.1.1 tetrapolyphosphate + H2O 25.2% activity compared to the activity with diphosphate Pyrococcus horikoshii DSM 12428 ? - ? 141181 3.6.1.1 thiamine diphosphate + H2O low activity Arabidopsis thaliana ? - ? 93336 3.6.1.1 thiamine diphosphate + H2O low activity Arabidopsis thaliana Col-0 ? - ? 93336 3.6.1.1 triphosphate + H2O - Sulfolobus acidocaldarius 3 phosphate - ? 383385 3.6.1.1 triphosphate + H2O - Tribolium castaneum 3 phosphate - ? 383385 3.6.1.1 triphosphate + H2O - Rhipicephalus microplus 3 phosphate - ? 383385 3.6.1.1 triphosphate + H2O 3-6% of the activity with diphosphate Sulfolobus acidocaldarius ? + phosphate - ? 393717 3.6.1.1 triphosphate + H2O - Trypanosoma cruzi ? - ? 398456 3.6.1.1 triphosphate + H2O - Pyrococcus furiosus ? - ? 398456 3.6.1.1 triphosphate + H2O the enzyme is less efficient for the hydrolysis of triphosphate than for diphosphate but is the main enzyme responsible for triphosphate hydrolysis in vivo Escherichia coli ? - ? 398456 3.6.1.1 triphosphate + H2O the enzyme is less efficient for the hydrolysis of triphosphate than for diphosphate but is the main enzyme responsible for triphosphate hydrolysis in vivo Escherichia coli MG1655 ? - ? 398456 3.6.1.1 tripolyphosphate + H2O - Escherichia coli ? - ? 141180 3.6.1.1 tripolyphosphate + H2O very weak substrate compared to diphosphate Sorghum bicolor ? - ? 141180 3.6.1.1 tripolyphosphate + H2O hydrolysis only with Zn2+ or Mn2+ as cofactors Saccharomyces cerevisiae ? - ? 141180 3.6.1.1 tripolyphosphate + H2O reaction only in the presence of Mn2+ or Zn2+ Saccharomyces cerevisiae ? - ? 141180 3.6.1.1 tripolyphosphate + H2O hydrolyzed at 44.3% of the rate compared to diphosphate Sulfolobus acidocaldarius ? - ? 141180 3.6.1.1 tripolyphosphate + H2O 40.3% activity compared to the activity with diphosphate Pyrococcus horikoshii ? - ? 141180 3.6.1.1 tripolyphosphate + H2O 40.3% activity compared to the activity with diphosphate Pyrococcus horikoshii DSM 12428 ? - ? 141180 3.6.1.1 tripolyphosphate + H2O hydrolyzed at 44.3% of the rate compared to diphosphate Sulfolobus acidocaldarius 7 ? - ? 141180 3.6.1.1 TTP + H2O 10% of the activity with diphosphate Sulfolobus acidocaldarius ? + phosphate - ? 393746 3.6.1.1 UDP + H2O - Pyrococcus furiosus ? - ? 73527 3.6.1.1 UDP-glucose + H2O - Pyrococcus furiosus ? - ? 382919 3.6.1.1 UTP + H2O 32% relative activity compared to diphosphate Kitasatospora aureofaciens ? - ? 73532 3.6.1.1 UTP + H2O weakest substrate Pyrococcus furiosus ? - ? 73532 3.6.1.1 UTP + H2O 32% relative activity compared to diphosphate Kitasatospora aureofaciens RIA 57 ? - ? 73532 3.6.1.1 UTP + H2O 32% relative activity compared to diphosphate Kitasatospora aureofaciens RIA57 ? - ? 73532