3.4.22.33 angiotensin II + H2O cleavage between Tyr4-Ile5 Ananas comosus hydrolyzed angiotensin II cleavage between Tyr4-Ile5 ? 16114 3.4.22.33 Azoalbumin + H2O - Ananas comosus ? - ? 16668 3.4.22.33 azocasein + H2O - Ananas comosus ? - ? 15347 3.4.22.33 Benzoyl-Gly-Gly-Lys + H2O enzyme form FBA is much more active than FBB Ananas comosus ? - ? 16125 3.4.22.33 benzoyl-L-arginine ethyl ester + H2O artificial substrate Ananas comosus ? - ? 404474 3.4.22.33 benzoyl-Phe-Val-Arg-4-methylcoumarin 7-amide + H2O convenient substrate for fruit bromelain, scarcely affected by stem bromelain Ananas comosus benzoyl-Phe-Val-Arg + 7-amino-4-methylcoumarin - ? 361854 3.4.22.33 benzoyl-Phe-Val-Arg-p-nitroanilide + H2O - Ananas comosus benzoyl-Phe-Val-Arg + p-nitroaniline - ? 36951 3.4.22.33 benzyl-Phe-Val-Arg-4-nitroanilide + H2O preferred substrate of fruit bromelain Ananas comosus benzyl-Phe-Val-Arg + 4-nitroaniline - ? 433499 3.4.22.33 Benzyloxycarbonyl-Ala-Leu + H2O enzyme form FBA is much more active than FBB Ananas comosus Benzyloxycarbonyl-Ala + Leu - ? 16124 3.4.22.33 benzyloxycarbonyl-Arg-Arg-4-nitroanilide + H2O - Ananas comosus benzyloxycarbonyl-Arg-Arg + 4-nitroaniline - ? 366770 3.4.22.33 benzyloxycarbonyl-Arg-Arg-p-nitroanilide + H2O - Ananas comosus benzyloxycarbonyl-Arg-Arg + p-nitroaniline - ? 36941 3.4.22.33 Benzyloxycarbonyl-Gly-Leu + H2O enzyme form FBA is much more active than FBB Ananas comosus Benzyloxycarbonyl-Gly + Leu - ? 16121 3.4.22.33 Benzyloxycarbonyl-Gly-Phe + H2O - Ananas comosus Benzyloxycarbonyl-Gly + Phe - ? 16122 3.4.22.33 Benzyloxycarbonyl-Gly-Tyr + H2O - Ananas comosus Benzyloxycarbonyl-Gly + Tyr - ? 16123 3.4.22.33 Benzyloxycarbonyl-Gly-Val + H2O enzyme form FBA is much more active than FBB Ananas comosus Benzyloxycarbonyl-Gly + Val - ? 16120 3.4.22.33 benzyloxyxarbonyl-Phe-Arg-7-amido-4-methylcoumarin + H2O - Bromelia fastuosa ? - ? 391472 3.4.22.33 bradykinin + H2O cleavage between Gly4-Phe5 and Phe5-Ser6 Ananas comosus fragments of bradykinin cleavage between Gly4-Phe5 and Phe5-Ser6 ? 16113 3.4.22.33 bromelain + H2O rapid self-digestion in aqueous solution, can be prevented by addition of alpha2-macroglobulin Ananas comosus ? - ? 366781 3.4.22.33 Carbobenzoxy-Gly-Ala + H2O enzyme form FBA is much more active than FBB Ananas comosus Carbobenzoxy-Gly + Ala - ? 15049 3.4.22.33 casein + H2O - Ananas comosus hydrolyzed casein - ? 15305 3.4.22.33 casein + H2O - Ananas comosus ? - ? 15445 3.4.22.33 casein + H2O - Bromelia fastuosa ? - ? 15445 3.4.22.33 casein + H2O enzyme is assayed using casein as a substrate with Coomassie dye to track completion of hydrolysis of casein, casein is hydrolysed to completion leading to a brown coloured solution, assay method development as a Bradford dye-binding-bromelain-casein system as an assay for heavy metals, based on colour development and its inhibition by Hg2+ and Cu2+ Ananas comosus ? - ? 15445 3.4.22.33 casein + H2O - Ananas comosus AGB 772 ? - ? 15445 3.4.22.33 casein + H2O - Ananas comosus L-Tyr + ? - ? 411849 3.4.22.33 casein + H2O - Ananas comosus tyrosine + ? - ? 449734 3.4.22.33 epsilon-NH2-Cap-Ala-Cys(S-Bzl)-7-amido-4-methylcoumarin + H2O - Bromelia fastuosa ? - ? 391917 3.4.22.33 epsilon-NH2-Cap-Cys(S-Bzl)-Leu-7-amido-4-methylcoumarin + H2O - Bromelia fastuosa ? - ? 391918 3.4.22.33 epsilon-NH2-Cap-Leu-Cys(S-Bzl)-7-amido-4-methylcoumarin + H2O - Bromelia fastuosa ? - ? 391919 3.4.22.33 epsilon-NH2-Cap-Leu-Gly-7-amido-4-methylcoumarin + H2O - Bromelia fastuosa ? - ? 391920 3.4.22.33 epsilon-NH2-Cap-Phe-Arg-7-amido-4-methylcoumarin + H2O - Bromelia fastuosa ? - ? 391921 3.4.22.33 epsilon-NH2-Cap-Pro-Cys(S-Bzl)-7-amido-4-methylcoumarin + H2O - Bromelia fastuosa ? - ? 391922 3.4.22.33 Fibrin + H2O - Ananas comosus ? - ? 16541 3.4.22.33 Fibrin + H2O fibrinolytic activity in vivo Ananas comosus ? - ? 16541 3.4.22.33 Gelatin + H2O - Ananas comosus ? - ? 16570 3.4.22.33 Hemoglobin + H2O - Ananas comosus ? - ? 15450 3.4.22.33 Hemoglobin + H2O - Ananas comosus Hydrolyzed hemoglobin - ? 15477 3.4.22.33 Isolated soybean protein + H2O - Ananas comosus Hydrolyzed soybean protein - ? 16116 3.4.22.33 milk protein + H2O about 62% hydrolysis Bromelia karatas ? - ? 95048 3.4.22.33 milk protein + H2O about 69% hydrolysis Bromelia pinguin ? - ? 95048 3.4.22.33 additional information specificity overview Ananas comosus ? - ? 89 3.4.22.33 additional information enzyme catalyzes synthesis of acylamino acid anilides Ananas comosus ? - ? 89 3.4.22.33 additional information bromelain has a profound immunmodulatory effect, overview, bromelain affects blood coagulation, fibrinolysis, and platelet functions, mechanism, overview Ananas comosus ? - ? 89 3.4.22.33 additional information enzyme is scarcely able to hydrolyze benzyloxycarbonyl-Arg-Arg-4-methylcoumarin 7-amide Ananas comosus ? - ? 89 3.4.22.33 additional information bromelain is a cysteine protease containing a single thiol group in the active sites Ananas comosus ? - ? 89 3.4.22.33 N-alpha-benzoyl-DL-arginine-2-naphthylamide + H2O - Bromelia fastuosa N-alpha-benzoyl-DL-arginine + 2-naphthylamine - ? 392749 3.4.22.33 N-Benzoyl-L-Arg ethyl ester + H2O - Ananas comosus N-Benzoyl-L-Arg + ethanol - ? 15729 3.4.22.33 N-benzoyl-L-tyrosine ethyl ester + H2O artificial substrate Ananas comosus ? - ? 401446 3.4.22.33 Nalpha-Benzoyl-Arg amide + H2O - Ananas comosus Nalpha-Benzoyl-Arg + NH3 - ? 16115 3.4.22.33 Nalpha-Benzoyl-DL-Arg 4-nitroanilide + H2O - Ananas comosus Nalpha-Benzoyl-DL-Arg + 4-nitroaniline - ? 16118 3.4.22.33 Nalpha-benzoyl-L-arginine-4-nitroanilide + H2O artificial substrate Ananas comosus ? - ? 406094 3.4.22.33 ovalbumin + H2O about 50% hydrolysis Bromelia karatas ? - ? 37064 3.4.22.33 ovalbumin + H2O about 66% hydrolysis Bromelia pinguin ? - ? 37064 3.4.22.33 protein + H2O broad substrate specificity Ananas comosus peptides - ? 365037 3.4.22.33 sodium caseinate + H2O - Ananas comosus ? - ? 432376 3.4.22.33 soybean protein + H2O about 73% hydrolysis Bromelia karatas ? - ? 450634 3.4.22.33 soybean protein + H2O about 82% hydrolysis Bromelia pinguin ? - ? 450634 3.4.22.33 Wheat gluten + H2O - Ananas comosus Hydrolyzed wheat gluten - ? 16108