3.2.1.91 2,4-dinitrophenyl cellobiose + H2O - Thermobifida fusca 2,4-dinitrophenol + cellobiose - ? 411185 3.2.1.91 2-chloro-4-nitrophenyl-beta-D-cellotrioside + H2O - Rasamsonia emersonii 2-chloro-4-nitrophenol + ? - ? 444628 3.2.1.91 2-chloronitrophenyl-beta-D-lactoside + H2O - Acetivibrio thermocellus ? - ? 371651 3.2.1.91 4-methylumbelliferyl beta-cellobioside + H2O substrate binding analysis of wild-type enzyme and mutant D274A, mutant D274A binds 4-methylumbelliferyl beta-cellobioside to approximately the same extent as the wild-type, overview Thermobifida fusca 4-methylumbelliferol + cellobiose - ? 411310 3.2.1.91 4-methylumbelliferyl beta-D-cellobioside + H2O - Thermotoga sp. ? - ? 115827 3.2.1.91 4-methylumbelliferyl beta-D-cellobioside + H2O - Thermotoga sp. FjSS3-B.1 ? - ? 115827 3.2.1.91 4-methylumbelliferyl beta-D-cellobioside + H2O - Phanerodontia chrysosporium 4-methylumbelliferol + beta-D-cellobiose - ? 381147 3.2.1.91 4-methylumbelliferyl beta-D-cellobioside + H2O - Caldicellulosiruptor saccharolyticus 4-methylumbelliferone + beta-D-cellobiose - ? 431371 3.2.1.91 4-methylumbelliferyl beta-D-cellobioside + H2O - Trichoderma virens 4-methylumbelliferone + cellobiose - ? 431372 3.2.1.91 4-methylumbelliferyl beta-D-cellobioside + H2O - Neocallimastix patriciarum 4-methylumbelliferone + cellobiose - ? 431372 3.2.1.91 4-methylumbelliferyl beta-D-cellobioside + H2O - Thermochaetoides thermophila 4-methylumbelliferone + cellobiose - ? 431372 3.2.1.91 4-methylumbelliferyl beta-D-cellobioside + H2O - Aspergillus nidulans 4-methylumbelliferone + cellobiose - ? 431372 3.2.1.91 4-methylumbelliferyl beta-D-cellobioside + H2O - Aspergillus nidulans ATCC 38163 4-methylumbelliferone + cellobiose - ? 431372 3.2.1.91 4-methylumbelliferyl beta-D-cellobioside + H2O - Trichoderma virens UKM1 4-methylumbelliferone + cellobiose - ? 431372 3.2.1.91 4-methylumbelliferyl beta-D-lactopyranoside + H2O - Aspergillus nidulans 4-methylumbelliferone + lactose - ? 431373 3.2.1.91 4-methylumbelliferyl beta-D-lactopyranoside + H2O - Aspergillus nidulans ATCC 38163 4-methylumbelliferone + lactose - ? 431373 3.2.1.91 4-methylumbelliferyl beta-D-lactoside + H2O - Phanerodontia chrysosporium 4-methylumbelliferol + beta-D-lactose - ? 381148 3.2.1.91 4-methylumbelliferyl-beta-D-cellobioside + H2O - Acetivibrio thermocellus ? - ? 372011 3.2.1.91 4-methylumbelliferyl-beta-D-cellobioside + H2O - Trichoderma viride 4-methylumbelliferol + cellobiose - ? 390865 3.2.1.91 4-methylumbelliferyl-beta-D-cellohexaoside + H2O - Acetivibrio thermocellus ? - ? 372012 3.2.1.91 4-methylumbelliferyl-beta-D-cellopentaoside + H2O - Acetivibrio thermocellus ? - ? 372013 3.2.1.91 4-methylumbelliferyl-beta-D-cellotetraoside + H2O - Acetivibrio thermocellus ? - ? 372014 3.2.1.91 4-methylumbelliferyl-beta-D-cellotrioside + H2O - Acetivibrio thermocellus ? - ? 372015 3.2.1.91 4-methylumbelliferyl-beta-D-oligoglucosides + H2O enzyme preferentially cleaves the second or the fourth glycosidic bond, cleaves the second bond between putative sub-sites -2 and -1 or +1 and +2, cleaves the fourth bond between putative sub-sites -1 and +1 Acetivibrio thermocellus ? - ? 372025 3.2.1.91 4-nitrophenyl beta-cellobioside + H2O - Phanerodontia chrysosporium 4-nitrophenol + beta-cellobiose - ? 381177 3.2.1.91 4-nitrophenyl beta-D-cellobioside + H2O - Trichoderma reesei 4-nitrophenol + beta-D-cellobiose - ? 372058 3.2.1.91 4-nitrophenyl beta-D-cellobioside + H2O - Penicillium chrysogenum 4-nitrophenol + beta-D-cellobiose - ? 372058 3.2.1.91 4-nitrophenyl beta-D-cellobioside + H2O substrate of wild-type enzyme and recombinant Cex-RsaA fusion mutant Cellulomonas fimi 4-nitrophenol + beta-D-cellobiose - ? 372058 3.2.1.91 4-nitrophenyl beta-D-cellobioside + H2O - Trichoderma reesei QM9414 4-nitrophenol + beta-D-cellobiose - ? 372058 3.2.1.91 4-nitrophenyl beta-D-cellobioside + H2O - Penicillium chrysogenum FS010 4-nitrophenol + beta-D-cellobiose - ? 372058 3.2.1.91 4-nitrophenyl beta-D-cellobioside + H2O - Acetivibrio thermocellus 4-nitrophenol + cellobiose - ? 372072 3.2.1.91 4-nitrophenyl beta-D-cellobioside + H2O - Trichoderma virens 4-nitrophenol + cellobiose - ? 372072 3.2.1.91 4-nitrophenyl beta-D-cellobioside + H2O - Rasamsonia emersonii 4-nitrophenol + cellobiose - ? 372072 3.2.1.91 4-nitrophenyl beta-D-cellobioside + H2O - Chrysosporium lucknowense 4-nitrophenol + cellobiose - ? 372072 3.2.1.91 4-nitrophenyl beta-D-cellobioside + H2O - Thermotoga petrophila 4-nitrophenol + cellobiose - ? 372072 3.2.1.91 4-nitrophenyl beta-D-cellobioside + H2O - Trichoderma reesei 4-nitrophenol + cellobiose - ? 372072 3.2.1.91 4-nitrophenyl beta-D-cellobioside + H2O - Caldicellulosiruptor saccharolyticus 4-nitrophenol + cellobiose - ? 372072 3.2.1.91 4-nitrophenyl beta-D-cellobioside + H2O - Halorhabdus utahensis 4-nitrophenol + cellobiose - ? 372072 3.2.1.91 4-nitrophenyl beta-D-cellobioside + H2O best substrate Talaromyces purpureogenus 4-nitrophenol + cellobiose - ? 372072 3.2.1.91 4-nitrophenyl beta-D-cellobioside + H2O 100% activity, highly preferred substrate Fomitopsis pinicola 4-nitrophenol + cellobiose - ? 372072 3.2.1.91 4-nitrophenyl beta-D-cellobioside + H2O - Trichoderma virens UKM1 4-nitrophenol + cellobiose - ? 372072 3.2.1.91 4-nitrophenyl beta-D-cellobioside + H2O best substrate Talaromyces purpureogenus KJS506 4-nitrophenol + cellobiose - ? 372072 3.2.1.91 4-nitrophenyl beta-D-cellobioside + H2O - Halorhabdus utahensis DSM 12940 4-nitrophenol + cellobiose - ? 372072 3.2.1.91 4-nitrophenyl beta-D-cellobioside + H2O - Chrysosporium lucknowense UV18-25 4-nitrophenol + cellobiose - ? 372072 3.2.1.91 4-nitrophenyl beta-D-cellobioside + H2O - Thermoascus aurantiacus 4-nitrophenol + D-cellobiose - ? 403932 3.2.1.91 4-nitrophenyl beta-D-galactopyranoside + H2O 0.26% activity compared to 4-nitrophenyl beta-D-cellobioside Fomitopsis pinicola 4-nitrophenol + D-galactopyranose - ? 416373 3.2.1.91 4-nitrophenyl beta-D-glucopyranoside + H2O 0.11% activity compared to 4-nitrophenyl beta-D-cellobioside Fomitopsis pinicola 4-nitrophenol + D-glucopyranose - ? 94269 3.2.1.91 4-nitrophenyl beta-D-glucopyranoside + H2O 0.11% activity compared to 4-nitrophenyl beta-D-cellobioside Fomitopsis pinicola KMJ812 4-nitrophenol + D-glucopyranose - ? 94269 3.2.1.91 4-nitrophenyl beta-D-lactopyranoside + H2O - Trichoderma virens 4-nitrophenol + beta-D-lactopyranose - ? 446783 3.2.1.91 4-nitrophenyl beta-D-lactopyranoside + H2O - Talaromyces pinophilus 4-nitrophenol + beta-D-lactopyranose - ? 446783 3.2.1.91 4-nitrophenyl beta-D-lactopyranoside + H2O - Trichoderma virens UKM1 4-nitrophenol + beta-D-lactopyranose - ? 446783 3.2.1.91 4-nitrophenyl beta-D-lactoside + H2O - Thermothelomyces thermophilus 4-nitrophenol + lactose - ? 372074 3.2.1.91 4-nitrophenyl beta-D-lactoside + H2O 42.1% activity compared to 4-nitrophenyl beta-D-cellobioside Fomitopsis pinicola 4-nitrophenol + lactose - ? 372074 3.2.1.91 4-nitrophenyl beta-D-lactoside + H2O - Thermothelomyces thermophilus ATCC 42464 4-nitrophenol + lactose - ? 372074 3.2.1.91 4-nitrophenyl beta-D-mannopyranoside + H2O 0.64% activity compared to 4-nitrophenyl beta-D-cellobioside Fomitopsis pinicola 4-nitrophenol + D-mannopyranose - ? 403958 3.2.1.91 4-nitrophenyl beta-D-xylopyranoside + H2O 0.64% activity compared to 4-nitrophenyl beta-D-cellobioside Fomitopsis pinicola 4-nitrophenol + D-xylopyranose - ? 411374 3.2.1.91 4-nitrophenyl beta-lactoside + H2O - Trichoderma reesei 4-nitrophenol + ? - ? 372062 3.2.1.91 4-nitrophenyl beta-lactoside + H2O - Phanerodontia chrysosporium 4-nitrophenol + beta-lactose - ? 381187 3.2.1.91 4-nitrophenyl cellobioside + H2O - Acetivibrio thermocellus 4-nitrophenol + cellobiose - ? 372063 3.2.1.91 4-nitrophenyl cellobioside + H2O - Acetivibrio thermocellus JW20 4-nitrophenol + cellobiose - ? 372063 3.2.1.91 4-nitrophenyl cellopentaoside + H2O - Acetivibrio thermocellus 4-nitrophenol + cellobiose + D-glucose - ? 372077 3.2.1.91 4-nitrophenyl cellopentaoside + H2O - Streptomyces coelicolor cellobiose + 4-nitrophenyl cellotrioside - ? 446797 3.2.1.91 4-nitrophenyl cellotetraoside + H2O - Streptomyces coelicolor cellobiose + 4-nitrophenyl cellobioside - ? 372079 3.2.1.91 4-nitrophenyl cellotetraoside + H2O - Acetivibrio thermocellus cellobiose + 4-nitrophenyl cellobioside - ? 372079 3.2.1.91 4-nitrophenyl cellotrioside + H2O - Acetivibrio thermocellus 4-nitrophenol + cellobiose + D-glucose - ? 372081 3.2.1.91 4-nitrophenyl lactoside + H2O - Trichoderma reesei 4-nitrophenol + lactose - ? 435155 3.2.1.91 4-nitrophenyl-beta-D-lactoside - Rasamsonia emersonii 4-nitrophenol + lactose - ? 444794 3.2.1.91 acid-swollen avicel + H2O - Orpinomyces sp. cellobiose + cellotriose - ? 435289 3.2.1.91 acid-swollen avicel + H2O - Orpinomyces sp. Y102 cellobiose + cellotriose - ? 435289 3.2.1.91 agarose + H2O low activity Streptomyces coelicolor ? - ? 372277 3.2.1.91 agarose + H2O about 40% activity compared to Avicel Streptomyces coelicolor ? - ? 372277 3.2.1.91 alpha-cellobiosyl fluoride + H2O - Humicola insolens fluoride + cellobiose - ? 372301 3.2.1.91 alpha-cellobiosyl fluoride + H2O - Trichoderma reesei fluoride + cellobiose - ? 372301 3.2.1.91 avicel + H2O - Talaromyces purpureogenus ? - ? 259817 3.2.1.91 avicel + H2O - Humicola insolens ? - ? 259817 3.2.1.91 avicel + H2O - Trichoderma reesei ? - ? 259817 3.2.1.91 avicel + H2O - Irpex lacteus ? - ? 259817 3.2.1.91 avicel + H2O - Trichoderma sp. ? - ? 259817 3.2.1.91 avicel + H2O - Trichoderma virens ? - ? 259817 3.2.1.91 avicel + H2O - Talaromyces pinophilus ? - ? 259817 3.2.1.91 avicel + H2O - Phanerodontia chrysosporium ? - ? 259817 3.2.1.91 avicel + H2O - Thermothelomyces thermophilus ? - ? 259817 3.2.1.91 avicel + H2O - Aspergillus niger ? - ? 259817 3.2.1.91 avicel + H2O shows a synergistic effect in the degradation of avicel when the enzyme acts together with either cellobiohydrolase I (Ex-1) or endoglucanase (En-1) produced by Irpex lacteus MC-2 Irpex lacteus ? - ? 259817 3.2.1.91 avicel + H2O microcrystalline cellulose Phanerodontia chrysosporium ? - ? 259817 3.2.1.91 avicel + H2O microcrystalline cellulose Trichoderma reesei ? - ? 259817 3.2.1.91 avicel + H2O microcrystalline cellulose Humicola insolens ? - ? 259817 3.2.1.91 avicel + H2O 26.9% activity compared to 4-nitrophenyl beta-D-cellobioside Fomitopsis pinicola ? - ? 259817 3.2.1.91 avicel + H2O cellobiohydrolase I when acting on Avicel, is not perfectly processive, the cellulose chain is released from the active site after 5-10 cellobiose units have been cleaved off. Adsorption of endoglucanase II and cellobiohydrolase I iss mutually negatively affected by the presence of the other enzyme. This can be the result of (i) depletion (hydrolysis) of common binding sites or (ii) competition for (occupation of) common adsorption sites. Importance of competition is shown by the concentration dependence of the observed decrease in adsorption. Cellobiohydrolase I is competing more efficiently for the binding sites than endoglucanase II. Adsorption results indicate that, besides common adsorption sites (for which endoglucanase II and cellobiohydrolase I compete), the enzymes have specific sites as well Trichoderma reesei ? - ? 259817 3.2.1.91 avicel + H2O - Thermothelomyces thermophilus JCP 1-4 ? - ? 259817 3.2.1.91 avicel + H2O shows a synergistic effect in the degradation of avicel when the enzyme acts together with either cellobiohydrolase I (Ex-1) or endoglucanase (En-1) produced by Irpex lacteus MC-2 Irpex lacteus MC-2 ? - ? 259817 3.2.1.91 avicel + H2O - Trichoderma virens UKM1 ? - ? 259817 3.2.1.91 avicel + H2O - Aspergillus niger ATCC 64973 ? - ? 259817 3.2.1.91 avicel + H2O - Talaromyces purpureogenus KJS506 ? - ? 259817 3.2.1.91 avicel + H2O - Talaromyces purpureogenus cellobiose - ? 259931 3.2.1.91 avicel + H2O - Penicillium sp. cellobiose - ? 259931 3.2.1.91 avicel + H2O - Fusarium oxysporum cellobiose - ? 259931 3.2.1.91 avicel + H2O - Cellulomonas uda cellobiose - ? 259931 3.2.1.91 avicel + H2O - Acetivibrio thermocellus cellobiose - ? 259931 3.2.1.91 avicel + H2O - Coprinopsis cinerea cellobiose - ? 259931 3.2.1.91 avicel + H2O - Thermoclostridium stercorarium cellobiose - ? 259931 3.2.1.91 avicel + H2O - Irpex lacteus cellobiose plus traces of cellotriose ? 259931 3.2.1.91 avicel + H2O highest activity Streptomyces coelicolor cellobiose - ? 259931 3.2.1.91 avicel + H2O high activity Streptomyces coelicolor cellobiose - ? 259931 3.2.1.91 avicel + H2O low specific activity Neocallimastix patriciarum cellobiose - ? 259931 3.2.1.91 avicel + H2O 191% activity compared to cellulose Pyricularia oryzae cellobiose - ? 259931 3.2.1.91 avicel + H2O - Coprinopsis cinerea 5338 cellobiose - ? 259931 3.2.1.91 avicel + H2O - Acetivibrio thermocellus CBH III cellobiose - ? 259931 3.2.1.91 avicel + H2O low specific activity Neocallimastix patriciarum J11 cellobiose - ? 259931 3.2.1.91 avicel + H2O - Humicola insolens cellobiose + ? - ? 391390 3.2.1.91 avicel + H2O - Trichoderma reesei cellobiose + ? - ? 391390 3.2.1.91 avicel + H2O - Thermochaetoides thermophila cellobiose + ? - ? 391390 3.2.1.91 avicel + H2O - Rasamsonia emersonii cellobiose + ? - ? 391390 3.2.1.91 avicel + H2O - Chrysosporium lucknowense cellobiose + ? - ? 391390 3.2.1.91 avicel + H2O - Caldicellulosiruptor saccharolyticus cellobiose + ? - ? 391390 3.2.1.91 avicel + H2O - Trichocladium griseum cellobiose + ? - ? 391390 3.2.1.91 avicel + H2O - Aspergillus nidulans cellobiose + ? - ? 391390 3.2.1.91 avicel + H2O - Aspergillus nidulans ATCC 38163 cellobiose + ? - ? 391390 3.2.1.91 avicel + H2O - Chrysosporium lucknowense UV18-25 cellobiose + ? - ? 391390 3.2.1.91 avicel + H2O microcrystalline cellulose, Avicel PH-101, at 2% Thermoascus aurantiacus D-glucose + ? - ? 435345 3.2.1.91 Avicel PH-101 + H2O - Trichoderma reesei ? - ? 431640 3.2.1.91 Avicel PH-101 + H2O - Trichoderma reesei QM9414 ? - ? 431640 3.2.1.91 Avicel PH101 + H2O - Trichoderma reesei cellobiose + ? - ? 445249 3.2.1.91 barley beta-glucan + H2O - Orpinomyces sp. ? - ? 372495 3.2.1.91 barley beta-glucan + H2O highest specific activity Neocallimastix patriciarum ? - ? 372495 3.2.1.91 barley beta-glucan + H2O - Orpinomyces sp. Y102 ? - ? 372495 3.2.1.91 barley beta-glucan + H2O highest specific activity Neocallimastix patriciarum J11 ? - ? 372495 3.2.1.91 barley straw + H2O - Trichoderma reesei ? - ? 391402 3.2.1.91 beechwood xylan + H2O low activity Streptomyces coelicolor ? - ? 391405 3.2.1.91 beechwood xylan + H2O about 20% activity compared to Avicel Streptomyces coelicolor ? - ? 391405 3.2.1.91 beta-cellobiosyl fluoride + H2O - Humicola insolens fluoride + cellobiose - ? 372539 3.2.1.91 beta-cellobiosyl fluoride + H2O - Trichoderma reesei fluoride + cellobiose - ? 372539 3.2.1.91 beta-D-glucan + H2O highest activity Thermochaetoides thermophila ? - ? 404515 3.2.1.91 beta-D-glucan + H2O highest activity Thermochaetoides thermophila DSM 1495 ? - ? 404515 3.2.1.91 beta-glucan + H2O low activity Streptomyces coelicolor ? - ? 94321 3.2.1.91 beta-glucan + H2O 227% activity compared to cellulose Pyricularia oryzae ? - ? 94321 3.2.1.91 beta-glucan + H2O low activity Streptomyces coelicolor cellobiose - ? 447234 3.2.1.91 carboxymethyl cellulose + H2O - Irpex lacteus ? - ? 94663 3.2.1.91 carboxymethyl cellulose + H2O - Orpinomyces sp. ? - ? 94663 3.2.1.91 carboxymethyl cellulose + H2O - Phanerodontia chrysosporium ? - ? 94663 3.2.1.91 carboxymethyl cellulose + H2O - Caldicellulosiruptor saccharolyticus ? - ? 94663 3.2.1.91 carboxymethyl cellulose + H2O - Xanthomonas oryzae pv. oryzae ? - ? 94663 3.2.1.91 carboxymethyl cellulose + H2O low specific activity Neocallimastix patriciarum ? - ? 94663 3.2.1.91 carboxymethyl cellulose + H2O lowest activity Thermochaetoides thermophila ? - ? 94663 3.2.1.91 carboxymethyl cellulose + H2O - Orpinomyces sp. Y102 ? - ? 94663 3.2.1.91 carboxymethyl cellulose + H2O lowest activity Thermochaetoides thermophila DSM 1495 ? - ? 94663 3.2.1.91 carboxymethyl cellulose + H2O - Irpex lacteus MC-2 ? - ? 94663 3.2.1.91 carboxymethyl cellulose + H2O low specific activity Neocallimastix patriciarum J11 ? - ? 94663 3.2.1.91 carboxymethyl cellulose + H2O - Xanthomonas oryzae pv. oryzae BXO43 ? - ? 94663 3.2.1.91 carboxymethyl cellulose + H2O - Halorhabdus utahensis cellobiose + ? - ? 391571 3.2.1.91 carboxymethyl cellulose + H2O high activity Streptomyces coelicolor cellobiose + ? - ? 391571 3.2.1.91 carboxymethyl cellulose + H2O - Halorhabdus utahensis DSM 12940 cellobiose + ? - ? 391571 3.2.1.91 carboxymethyl cellulose + H2O 97% activity compared to Avicel Streptomyces coelicolor cellobiose - ? 441159 3.2.1.91 carboxymethylcellulose + H2O 0.26% activity compared to 4-nitrophenyl beta-D-cellobioside Fomitopsis pinicola ? - ? 115813 3.2.1.91 carboxymethylcellulose + H2O - Penicillium sp. cellobiose + ? - ? 259929 3.2.1.91 carboxymethylcellulose + H2O - Trichoderma reesei cellobiose + ? - ? 259929 3.2.1.91 carboxymethylcellulose + H2O - Thermobifida fusca cellobiose + ? - ? 259929 3.2.1.91 carboxymethylcellulose + H2O - Thermochaetoides thermophila cellobiose + ? - ? 259929 3.2.1.91 carboxymethylcellulose + H2O - Thermotoga sp. cellobiose + ? - ? 259929 3.2.1.91 carboxymethylcellulose + H2O - Thermoclostridium stercorarium cellobiose + ? - ? 259929 3.2.1.91 carboxymethylcellulose + H2O - Trichoderma reesei cellobiose + ? CBH I, exo-glucanase ? 259929 3.2.1.91 carboxymethylcellulose + H2O little activity Trichoderma koningii cellobiose + ? - ? 259929 3.2.1.91 carboxymethylcellulose + H2O - Thermotoga sp. FjSS3-B.1 cellobiose + ? - ? 259929 3.2.1.91 carboxymethylcellulose + H2O - Niallia circulans glucose + cellobiose + cellutetraose - ? 259930 3.2.1.91 carboxymethylcellulose + H2O - Acetivibrio thermocellus glucose + cellobiose + cellutetraose - ? 259930 3.2.1.91 carboxymethylcellulose + H2O - Trichoderma reesei glucose + cellobiose + cellutetraose CBH II, endo-glucanase ? 259930 3.2.1.91 carboxymethylcellulose + H2O - Acetivibrio thermocellus CBH III glucose + cellobiose + cellutetraose - ? 259930 3.2.1.91 cello-oligosaccharides + H2O natural and derivatized cello-oligosaccharides, H3PO4-swollen cellulose, Avicel, laminarin, lichenan, barley glucan Acetivibrio thermocellus ? - ? 372630 3.2.1.91 cellobiose + H2O 0.11% activity compared to 4-nitrophenyl beta-D-cellobioside Fomitopsis pinicola ? - ? 372639 3.2.1.91 cellodextrins + H2O - Thermothelomyces heterothallicus cellobiose + D-glucose + cellotriose - ? 259925 3.2.1.91 cellohexaose + 3 H2O complete degradation within 30 min, 50°C Thermothielavioides terrestris 3 cellobiose - ? 435389 3.2.1.91 cellohexaose + 3 H2O complete degradation within 30 min, 50°C Thermothielavioides terrestris ATCC 3808 3 cellobiose - ? 435389 3.2.1.91 cellohexaose + H2O - Niallia circulans cellotriose + cellobiose + cellotetraose - ? 259933 3.2.1.91 cellohexaose + H2O - Irpex lacteus cellotriose + cellobiose + cellotetraose - ? 259933 3.2.1.91 cellohexaose + H2O - Trichoderma koningii cellotriose + cellobiose + cellotetraose - ? 259933 3.2.1.91 cellohexaose + H2O - Trichoderma reesei ? - ? 372645 3.2.1.91 cellohexaose + H2O - Aspergillus ficuum cellobiose + cellotetraose - ? 372646 3.2.1.91 cellohexaose + H2O - Orpinomyces sp. cellobiose + cellotriose + cellotetraose enzyme does not release D-glucose ? 381633 3.2.1.91 cellohexaose + H2O - Orpinomyces sp. Y102 cellobiose + cellotriose + cellotetraose enzyme does not release D-glucose ? 381633 3.2.1.91 cellohexaose + H2O - Streptomyces coelicolor cellobiose + cellotetraose + cellotriose - ? 443274 3.2.1.91 celloligosaccharides + H2O - Fibrobacter succinogenes cellobiose + H2O - ? 259935 3.2.1.91 cellooligosaccharide + H2O degree of polymerization is 25 Aspergillus ficuum cellobiose - ? 416761 3.2.1.91 cellopentaose + H2O - Niallia circulans cellobiose + D-glucose + cellotriose - ? 259932 3.2.1.91 cellopentaose + H2O - Talaromyces purpureogenus cellobiose + D-glucose + cellotriose - ? 259932 3.2.1.91 cellopentaose + H2O - Irpex lacteus cellobiose + D-glucose + cellotriose - ? 259932 3.2.1.91 cellopentaose + H2O - Trichoderma reesei ? - ? 372650 3.2.1.91 cellopentaose + H2O - Streptomyces coelicolor cellobiose + cellotriose - ? 372651 3.2.1.91 cellopentaose + H2O good substrate Pyricularia oryzae cellobiose + cellotriose - ? 372651 3.2.1.91 cellopentaose + H2O - Acetivibrio thermocellus cellobiose + D-glucose - ? 372652 3.2.1.91 cellopentaose + H2O - Teredinibacter turnerae cellotriose + cellobiose - ? 391608 3.2.1.91 cellopentaose + H2O cellobiose is preferentialls released from the nonreducing end by exocellulase E3, cellopentaose is labeled at the reducing end with (18)O, the products are analyzed by ionspray mass spectrometry, 61% of the (18)O is found in cellotriose Thermobifida fusca cellotriose + cellobiose - ? 391608 3.2.1.91 cellopentaose + H2O cellobiose is preferentially released from the nonreducing end by CBH II, cellopentaose is labeled at the reducing end with (18)O, the products are analyzed by ionspray mass spectrometry, 63% of the (18)O is found in cellotriose Trichoderma reesei cellotriose + cellobiose - ? 391608 3.2.1.91 cellopentaose + H2O complete degradation within 30 min, 50°C Thermothielavioides terrestris cellotriose + cellobiose - ? 391608 3.2.1.91 cellopentaose + H2O complete degradation within 30 min, 50°C Thermothielavioides terrestris ATCC 3808 cellotriose + cellobiose - ? 391608 3.2.1.91 cellopentaose + H2O - Teredinibacter turnerae T7902 cellotriose + cellobiose - ? 391608 3.2.1.91 cellopentaose + H2O - Aspergillus ficuum cellobiose + ? - ? 416762 3.2.1.91 cellopentaose + H2O - Talaromyces purpureogenus cellobiose + ? cellobiose is the major product ? 416762 3.2.1.91 cellopentaose + H2O - Talaromyces purpureogenus KJS506 cellobiose + ? cellobiose is the major product ? 416762 3.2.1.91 cellotetraose + H2O - Streptomyces coelicolor 2 cellobiose - ? 259808 3.2.1.91 cellotetraose + H2O - Aspergillus ficuum 2 cellobiose - ? 259808 3.2.1.91 cellotetraose + H2O - Acetivibrio thermocellus 2 cellobiose - ? 259808 3.2.1.91 cellotetraose + H2O - Teredinibacter turnerae 2 cellobiose - ? 259808 3.2.1.91 cellotetraose + H2O complete degradation within 30 min, 50°C Thermothielavioides terrestris 2 cellobiose - ? 259808 3.2.1.91 cellotetraose + H2O - Streptomyces coelicolor A(3) 2 cellobiose - ? 259808 3.2.1.91 cellotetraose + H2O complete degradation within 30 min, 50°C Thermothielavioides terrestris ATCC 3808 2 cellobiose - ? 259808 3.2.1.91 cellotetraose + H2O - Teredinibacter turnerae T7902 2 cellobiose - ? 259808 3.2.1.91 cellotetraose + H2O - Talaromyces purpureogenus cellobiose + D-glucose + cellotriose - ? 259926 3.2.1.91 cellotetraose + H2O - Trichoderma koningii cellobiose + D-glucose + cellotriose - ? 259926 3.2.1.91 cellotetraose + H2O - Trichoderma reesei cellobiose + D-glucose + cellotriose - ? 259926 3.2.1.91 cellotetraose + H2O - Thermoclostridium stercorarium cellobiose + D-glucose + cellotriose - ? 259926 3.2.1.91 cellotetraose + H2O - Thermothelomyces heterothallicus cellobiose + D-glucose + cellotriose different combinations and proportions of products ? 259926 3.2.1.91 cellotetraose + H2O - Cellulomonas uda cellobiose + D-glucose + cellotriose different combinations and proportions of products ? 259926 3.2.1.91 cellotetraose + H2O - Niallia circulans cellobiose + D-glucose + cellotriose preferably cellobiose ? 259926 3.2.1.91 cellotetraose + H2O - Trichoderma reesei ? - ? 372657 3.2.1.91 cellotetraose + H2O - Talaromyces purpureogenus cellobiose + ? cellobiose is the major product ? 416763 3.2.1.91 cellotetraose + H2O good substrate Pyricularia oryzae cellobiose + ? - ? 416763 3.2.1.91 cellotriitol + H2O - Thermothelomyces heterothallicus cellobiose + sorbitol - ? 259928 3.2.1.91 cellotriose + H2O - Trichoderma reesei ? - ? 94515 3.2.1.91 cellotriose + H2O - Niallia circulans cellobiose + glucose - ? 259927 3.2.1.91 cellotriose + H2O - Fibrobacter succinogenes cellobiose + glucose - ? 259927 3.2.1.91 cellotriose + H2O - Irpex lacteus cellobiose + glucose - ? 259927 3.2.1.91 cellotriose + H2O - Thermothelomyces heterothallicus cellobiose + glucose - ? 259927 3.2.1.91 cellotriose + H2O - Acetivibrio thermocellus glucose + cellobiose - ? 372662 3.2.1.91 cellotriose + H2O - Aspergillus ficuum cellobiose + D-glucose - ? 435391 3.2.1.91 cellotriose + H2O complete degradation within 30 min, 50°C Thermothielavioides terrestris cellobiose + D-glucose - ? 435391 3.2.1.91 cellotriose + H2O complete degradation within 30 min, 50°C Thermothielavioides terrestris ATCC 3808 cellobiose + D-glucose - ? 435391 3.2.1.91 Celluclast-treated Avicel + H2O the recombinant enzyme is inactive with Avicel, but can digest Celluclast-treated Avicel producing cellobiose Thermothielavioides terrestris cellobiose + ? - ? 449743 3.2.1.91 cellulose + H2O - Acetivibrio thermocellus ? - ? 94577 3.2.1.91 cellulose + H2O - Trichoderma sp. ? - ? 94577 3.2.1.91 cellulose + H2O - Saccharophagus degradans ? - ? 94577 3.2.1.91 cellulose + H2O - Trichoderma reesei ? - ? 94577 3.2.1.91 cellulose + H2O cellobiohydrolases act from one end of a cellulose chain and processively cleave off cellobiose as the main product. Processivity and synergism are important properties of cellulases, particularly for hydrolysis of crystalline substrates Thermobifida fusca ? - ? 94577 3.2.1.91 cellulose + H2O substrates are phosphoric acid-treated cotton, carboxymethylcellulose, bacterial microcrystalline cellulose, and phosphoric acid-swollen cellulose, as well as 8-mg/ml Whatman no. 1 filter paper, substrate specificity of wild-type and mutant enzymes, overview Thermobifida fusca ? - ? 94577 3.2.1.91 cellulose + H2O - Saccharophagus degradans 2-40T ? - ? 94577 3.2.1.91 cellulose + H2O - Saccharophagus degradans 2-40 / ATCC 43961 ? - ? 94577 3.2.1.91 cellulose + H2O - Aspergillus niger cellobiose + D-glucose + cellotriose - ? 259924 3.2.1.91 cellulose + H2O - Trichoderma reesei cellobiose + D-glucose + cellotriose - ? 259924 3.2.1.91 cellulose + H2O - Phanerodontia chrysosporium cellobiose + D-glucose + cellotriose - ? 259924 3.2.1.91 cellulose + H2O - Acetivibrio thermocellus cellobiose + D-glucose + cellotriose - ? 259924 3.2.1.91 cellulose + H2O - Thermotoga sp. cellobiose + D-glucose + cellotriose - ? 259924 3.2.1.91 cellulose + H2O - Thermoclostridium stercorarium cellobiose + D-glucose + cellotriose - ? 259924 3.2.1.91 cellulose + H2O - Talaromyces purpureogenus cellobiose + D-glucose + cellotriose different combinations and proportions of products depending on organism ? 259924 3.2.1.91 cellulose + H2O - Ruminococcus albus cellobiose + D-glucose + cellotriose different combinations and proportions of products depending on organism ? 259924 3.2.1.91 cellulose + H2O - Fibrobacter succinogenes cellobiose + D-glucose + cellotriose different combinations and proportions of products depending on organism ? 259924 3.2.1.91 cellulose + H2O - Trichoderma viride cellobiose + D-glucose + cellotriose different combinations and proportions of products depending on organism ? 259924 3.2.1.91 cellulose + H2O - Penicillium sp. cellobiose + D-glucose + cellotriose different combinations and proportions of products depending on organism ? 259924 3.2.1.91 cellulose + H2O - Trichoderma harzianum cellobiose + D-glucose + cellotriose different combinations and proportions of products depending on organism ? 259924 3.2.1.91 cellulose + H2O - Trichoderma reesei cellobiose + D-glucose + cellotriose different combinations and proportions of products depending on organism ? 259924 3.2.1.91 cellulose + H2O - Fusarium oxysporum cellobiose + D-glucose + cellotriose different combinations and proportions of products depending on organism ? 259924 3.2.1.91 cellulose + H2O - Thermoclostridium stercorarium cellobiose + D-glucose + cellotriose different combinations and proportions of products depending on organism ? 259924 3.2.1.91 cellulose + H2O - Irpex lacteus cellobiose + D-glucose + cellotriose different combinations and proportions of products depending on organism ? 259924 3.2.1.91 cellulose + H2O - Athelia rolfsii cellobiose + D-glucose + cellotriose different combinations and proportions of products depending on organism ? 259924 3.2.1.91 cellulose + H2O - Thermothelomyces heterothallicus cellobiose + D-glucose + cellotriose different combinations and proportions of products depending on organism ? 259924 3.2.1.91 cellulose + H2O - Talaromyces funiculosus cellobiose + D-glucose + cellotriose different combinations and proportions of products depending on organism ? 259924 3.2.1.91 cellulose + H2O - Trichoderma koningii cellobiose + D-glucose + cellotriose different combinations and proportions of products depending on organism ? 259924 3.2.1.91 cellulose + H2O - Cellulomonas uda cellobiose + D-glucose + cellotriose different combinations and proportions of products depending on organism ? 259924 3.2.1.91 cellulose + H2O - Cellulomonas fimi cellobiose + D-glucose + cellotriose different combinations and proportions of products depending on organism ? 259924 3.2.1.91 cellulose + H2O - Ruminococcus flavefaciens cellobiose + D-glucose + cellotriose different combinations and proportions of products depending on organism ? 259924 3.2.1.91 cellulose + H2O - Talaromyces pinophilus cellobiose + D-glucose + cellotriose different combinations and proportions of products depending on organism ? 259924 3.2.1.91 cellulose + H2O - Niallia circulans cellobiose + D-glucose + cellotriose only cellobiose ? 259924 3.2.1.91 cellulose + H2O Cel6A is a more endo-processive enzyme, whereas Cel7A is essentially a processive enzyme, both forms show a synergistic effect in the digestion of bacterial cellulose Humicola insolens cellobiose + D-glucose + cellotriose - ? 259924 3.2.1.91 cellulose + H2O - Phanerodontia chrysosporium CBH I cellobiose + D-glucose + cellotriose - ? 259924 3.2.1.91 cellulose + H2O - Acetivibrio thermocellus CBH III cellobiose + D-glucose + cellotriose - ? 259924 3.2.1.91 cellulose + H2O - Trichoderma reesei Cel6A cellobiose + D-glucose + cellotriose - ? 259924 3.2.1.91 cellulose + H2O - Thermotoga sp. FjSS3-B.1 cellobiose + D-glucose + cellotriose - ? 259924 3.2.1.91 cellulose + H2O - Humicola insolens cellobiose + ? - ? 372665 3.2.1.91 cellulose + H2O - Trichoderma viride cellobiose + ? - ? 372665 3.2.1.91 cellulose + H2O - Trichoderma reesei cellobiose + ? - ? 372665 3.2.1.91 cellulose + H2O - Thermobifida fusca cellobiose + ? - ? 372665 3.2.1.91 cellulose + H2O - Thermochaetoides thermophila cellobiose + ? - ? 372665 3.2.1.91 cellulose + H2O - Rasamsonia emersonii cellobiose + ? - ? 372665 3.2.1.91 cellulose + H2O - Caldicellulosiruptor saccharolyticus cellobiose + ? - ? 372665 3.2.1.91 cellulose + H2O - Cellulomonas fimi cellobiose + ? - ? 372665 3.2.1.91 cellulose + H2O - Aspergillus nidulans cellobiose + ? - ? 372665 3.2.1.91 cellulose + H2O hydrolysis of filter paper by intact cellobiohydrolase I in the presence of additional cellulose binding domains has a synergistic effect, leading to an increase of the sugar production of up to 30% Trichoderma reesei cellobiose + ? - ? 372665 3.2.1.91 cellulose + H2O cellulolytic enzyme Cellulomonas fimi cellobiose + ? - ? 372665 3.2.1.91 cellulose + H2O the enzyme hydrolyzes beta-1,4-linkages Phanerodontia chrysosporium cellobiose + ? - ? 372665 3.2.1.91 cellulose + H2O the enzyme acts on anthranilic-acid-labelled bacterial cellulose, bacterial microcrystalline cellulose, and endoglucanase-pretreated bacterial cellulose, preparation, overview Trichoderma reesei cellobiose + ? - ? 372665 3.2.1.91 cellulose + H2O activity with bacterial microcrystalline cellulose, phosphoric acid-treated swollen cellulose, phosphoric acid-treated cotton, and carboxymethyl cellulose Thermobifida fusca cellobiose + ? - ? 372665 3.2.1.91 cellulose + H2O 100% activity with 5 mg cellulose, 30% activity with 0.5 mg cellulose as substrate Pyricularia oryzae cellobiose + ? - ? 372665 3.2.1.91 cellulose + H2O hydrolyses pure cellulose, either as highly crystalline native cotton or as highly hydrated amorphous cellulose. No activity with carboxymethylcellulose Thermothelomyces thermophilus cellobiose + ? - ? 372665 3.2.1.91 cellulose + H2O the enzyme acts on nonreducing end of cellulose Streptomyces coelicolor cellobiose + ? - ? 372665 3.2.1.91 cellulose + H2O hydrolyses pure cellulose, either as highly crystalline native cotton or as highly hydrated amorphous cellulose. No activity with carboxymethylcellulose Thermothelomyces thermophilus ATCC 42464 cellobiose + ? - ? 372665 3.2.1.91 cellulose + H2O - Aspergillus nidulans ATCC 38163 cellobiose + ? - ? 372665 3.2.1.91 cellulose + H2O - Trichoderma reesei QM9414 cellobiose + ? - ? 372665 3.2.1.91 cellulose + H2O the enzyme acts on anthranilic-acid-labelled bacterial cellulose, bacterial microcrystalline cellulose, and endoglucanase-pretreated bacterial cellulose, preparation, overview Trichoderma reesei QM9414 cellobiose + ? - ? 372665 3.2.1.91 cellulose + H2O hydrolyzes Avicel and filter paper, efficiency of hydrolysis increases in the following order: Gh9, Gh9-Fn31,2, Gh9-Fn31,2-CBDIII Acetivibrio thermocellus cellobiose + cellohexaose - ? 372667 3.2.1.91 cellulose + H2O - Trichoderma reesei beta-cellobiose + ? - ? 380571 3.2.1.91 cellulose + H2O - Trichoderma reesei RUT C30 beta-cellobiose + ? - ? 380571 3.2.1.91 cellulose nanowhisker + H2O crystalline portion of cellulose Trichoderma reesei cellobiose + ? - ? 445294 3.2.1.91 chito-oligosaccharide + H2O oligosaccharides with a degree of polymerization between three and six units, product analysis by analyzed by mass and NMR spectrometry Trichoderma reesei ? - ? 449757 3.2.1.91 chito-oligosaccharide + H2O oligosaccharides with a degree of polymerization between three and six units, product analysis by analyzed by mass and NMR spectrometry Trichoderma longibrachiatum ? - ? 449757 3.2.1.91 chito-oligosaccharide + H2O oligosaccharides with a degree of polymerization between three and six units, product analysis by analyzed by mass and NMR spectrometry Trichoderma reesei NRRL 11460 ? - ? 449757 3.2.1.91 chito-oligosaccharide + H2O oligosaccharides with a degree of polymerization between three and six units, product analysis by analyzed by mass and NMR spectrometry Trichoderma reesei BCRC 32924 ? - ? 449757 3.2.1.91 chito-oligosaccharide + H2O oligosaccharides with a degree of polymerization between three and six units, product analysis by analyzed by mass and NMR spectrometry Trichoderma reesei ATCC 56765 ? - ? 449757 3.2.1.91 chito-oligosaccharide + H2O oligosaccharides with a degree of polymerization between three and six units, product analysis by analyzed by mass and NMR spectrometry Trichoderma reesei RUT C-30 ? - ? 449757 3.2.1.91 chito-oligosaccharide + H2O oligosaccharides with a degree of polymerization between three and six units, product analysis by analyzed by mass and NMR spectrometry Trichoderma longibrachiatum ATCC 18648 ? - ? 449757 3.2.1.91 chitosan + H2O DA13 Trichoderma reesei N-acetyl-D-glucosamine + ? - ? 448503 3.2.1.91 chitosan + H2O DA13 Trichoderma longibrachiatum N-acetyl-D-glucosamine + ? - ? 448503 3.2.1.91 chitosan + H2O DA13 Trichoderma reesei NRRL 11460 N-acetyl-D-glucosamine + ? - ? 448503 3.2.1.91 chitosan + H2O DA13 Trichoderma reesei BCRC 32924 N-acetyl-D-glucosamine + ? - ? 448503 3.2.1.91 chitosan + H2O DA13 Trichoderma reesei ATCC 56765 N-acetyl-D-glucosamine + ? - ? 448503 3.2.1.91 chitosan + H2O DA13 Trichoderma reesei RUT C-30 N-acetyl-D-glucosamine + ? - ? 448503 3.2.1.91 chitosan + H2O DA13 Trichoderma longibrachiatum ATCC 18648 N-acetyl-D-glucosamine + ? - ? 448503 3.2.1.91 corn stover + H2O - Caldicellulosiruptor saccharolyticus cellobiose + ? - ? 416790 3.2.1.91 corncob residue + H2O - Aspergillus niger ? - ? 447343 3.2.1.91 corncob residue + H2O - Aspergillus niger ATCC 64973 ? - ? 447343 3.2.1.91 cotton + H2O - Trichoderma sp. ? - ? 372763 3.2.1.91 crystalline cellulose + H2O - Talaromyces pinophilus ? - ? 391699 3.2.1.91 filter paper + H2O - Talaromyces purpureogenus ? - ? 391981 3.2.1.91 filter paper + H2O - Trichoderma sp. ? - ? 391981 3.2.1.91 filter paper + H2O low activity Streptomyces coelicolor ? - ? 391981 3.2.1.91 filter paper + H2O about 18% activity compared to Avicel Streptomyces coelicolor ? - ? 391981 3.2.1.91 filter paper + H2O - Talaromyces purpureogenus KJS506 ? - ? 391981 3.2.1.91 galactan + H2O low activity Streptomyces coelicolor ? - ? 115929 3.2.1.91 galactan + H2O about 30% activity compared to Avicel Streptomyces coelicolor ? - ? 115929 3.2.1.91 galactan + H2O about 30% activity compared to Avicel Streptomyces coelicolor A(3) ? - ? 115929 3.2.1.91 laminarin + H2O 1.5% activity compared to 4-nitrophenyl beta-D-cellobioside Fomitopsis pinicola ? - ? 94671 3.2.1.91 laminarin + H2O 1.5% activity compared to 4-nitrophenyl beta-D-cellobioside Fomitopsis pinicola KMJ812 ? - ? 94671 3.2.1.91 lichenan + H2O - Thermochaetoides thermophila ? - ? 94498 3.2.1.91 lichenan + H2O - Acetivibrio thermocellus ? - ? 94498 3.2.1.91 lichenan + H2O high specific activity Neocallimastix patriciarum ? - ? 94498 3.2.1.91 lichenan + H2O 33.8% activity compared to 4-nitrophenyl beta-D-cellobioside Fomitopsis pinicola ? - ? 94498 3.2.1.91 lichenan + H2O 33.8% activity compared to 4-nitrophenyl beta-D-cellobioside Fomitopsis pinicola KMJ812 ? - ? 94498 3.2.1.91 lichenan + H2O - Acetivibrio thermocellus CBH III ? - ? 94498 3.2.1.91 lichenan + H2O high specific activity Neocallimastix patriciarum J11 ? - ? 94498 3.2.1.91 melibiose + H2O about 4% activity compared to Avicel Streptomyces coelicolor ? - ? 447752 3.2.1.91 methylumbelliferyl-lactoside + H2O - Trichoderma reesei methylumberlliferone + lactose - ? 259936 3.2.1.91 microcrystalline cellulose + H2O - Trichoderma reesei ? - ? 392651 3.2.1.91 additional information - Trichoderma viride ? - ? 89 3.2.1.91 additional information - Irpex lacteus ? - ? 89 3.2.1.91 additional information - Ruminococcus flavefaciens ? - ? 89 3.2.1.91 additional information substrate specificity, overview Phanerodontia chrysosporium ? - ? 89 3.2.1.91 additional information not: cellobiose Fibrobacter succinogenes ? - ? 89 3.2.1.91 additional information not: cellobiose Irpex lacteus ? - ? 89 3.2.1.91 additional information not: cellobiose Thermothelomyces heterothallicus ? - ? 89 3.2.1.91 additional information cellobiose slightly Trichoderma reesei ? - ? 89 3.2.1.91 additional information hydrolyses internal glucosidic linkages of beta-1,3, 1,4-D-glucan Irpex lacteus ? - ? 89 3.2.1.91 additional information no hydrolysis of a soluble cellulose derivative and barley (1-3),(1-4)-beta-D-glucan Rasamsonia emersonii ? - ? 89 3.2.1.91 additional information Cel5A treated cellulose is no longer a substrate for Cel6A or Cel7A Trichoderma reesei ? - ? 89 3.2.1.91 additional information has a multi domain structure composed of a cellulose-binding domain, a Ser/Thr/Pro-rich linker, and a catalytic domain belonging to family 6 of glycosyl hydrolases, from the N-terminus Irpex lacteus ? - ? 89 3.2.1.91 additional information no activity on oat spelt xylan, carboxymethylcellulose, xylobiose, cellobiose, 4-nitrophenyl-cellobiose, and 4-nitrophenyl derivatives of other disaccharides, overview Trichoderma reesei ? - ? 89 3.2.1.91 additional information no activity on oat spelt xylan, carboxymethylcellulose, xylobiose, cellobiose, and 4-nitrophenyl derivatives of other disaccharides, overview Trichoderma reesei ? - ? 89 3.2.1.91 additional information 4-nitrophenyl beta-D-glucopyranoside is not a substrate Caldicellulosiruptor saccharolyticus ? - ? 89 3.2.1.91 additional information does not hydrolyze carboxymethylcellulose Talaromyces purpureogenus ? - ? 89 3.2.1.91 additional information no activity with cellotriose, and cellotetraose Fomitopsis pinicola ? - ? 89 3.2.1.91 additional information the enzyme liberates cellobiose and cellotriose from the nonreducing ends and requires at least four contiguous beta-1,4-linked glucosyl units for a substrate but does not recognize sulforhodamine-conjugated 1-amino-1-deoxyglucitol. ulforhodamine-conjugated cellotetraose, carboxymethylcellulose, hydroxyethyl cellulose, xylan, and mannan are no substrates Pyricularia oryzae ? - ? 89 3.2.1.91 additional information no detectable activity on laminarin, 4-nitrophenyl-beta-D-glucopyranoside, oat spelt xylan, and birch wood xylan Neocallimastix patriciarum ? - ? 89 3.2.1.91 additional information no substrates: avicel, 4-methylumbelliferyl beta-D-cellobioside, 4-nitrophenyl beta-D-cellobioside, 4-nitrophenyl beta-D-lactopyranoside Thermothielavioides terrestris ? - ? 89 3.2.1.91 additional information no substrates: avicel, soluble oat spelt xylan, Pachyman, 4-nitrophenyl-beta-D-cellobioside, 4-nitrophenyl-beta-D-galactopyranoside Orpinomyces sp. ? - ? 89 3.2.1.91 additional information the enzyme completely lacks chromogenic activity on the 4-nitrophenyl-cellodextrins from 4-nitrophenyl glucoside to 4-nitrophenyl cellobioside Streptomyces coelicolor ? - ? 89 3.2.1.91 additional information binding constant to crystalline cellulose nanowhiskers is about 1000000 per M for Cel6A. For Avicel, lower binding constants are observed, and weak binding to phosphoric acid swollen cellulose. No binding of cellooligosaccharides of less than two glucose units Trichoderma reesei ? - ? 89 3.2.1.91 additional information Cel6A hydrolyzes cellulose chain from the nonreducing end, while the binding module (CBMCel6A) is likely binding to the reducing end Trichoderma reesei ? - ? 89 3.2.1.91 additional information enzyme degrades highly ordered crystalline forms of cellulose, i.e., cotton, microcrystalline cellulose, and Whatman CCAI cellulose, more readily than soluble polysaccharides such as lichenan, glucan, xylan and carboxymethylcellulose Thermochaetoides thermophila ? - ? 89 3.2.1.91 additional information no activity with 4-methylumbelliferyl beta-D-lactopyranoside or 4-methylumbelliferyl beta-D-cellobioside Aspergillus nidulans ? - ? 89 3.2.1.91 additional information no substrates: Avicel, cotton, cellulose powder. Enzyme splits off strictly cellobiose units from the nonreducing end of the cellulose chain with an exowise mechanism Aspergillus ficuum ? - ? 89 3.2.1.91 additional information compared to cellobiohydrolase Cel7A, cellobiohydrolase Cel6A is catalytically more efficient but less capable of attacking a broad range of structurally distinct sites on the cellulose surface Trichoderma reesei ? - ? 89 3.2.1.91 additional information no activity with cellotriose, alpha-cellulose, cellobiose, and raffinose. The enzyme completely lacks chromogenic activity on the 4-nitrophenyl cellodextrins from 4-nitrophenyl glucoside to 4-nitrophenyl cellobioside Streptomyces coelicolor ? - ? 89 3.2.1.91 additional information comparison of activities on chitosan by cellobiohydrolases, chitosanases, and lysozyme, oligomer pattern, overview. The different enzymes produce chito-oligosaccharides (COSs) with varying acetylation, NMR spectrometric analysis Trichoderma reesei ? - ? 89 3.2.1.91 additional information comparison of activities on chitosan by cellobiohydrolases, chitosanases, and lysozyme, oligomer pattern, overview. The different enzymes produce chito-oligosaccharides (COSs) with varying acetylation, NMR spectrometric analysis. The preferred cleavage site of enzyme CBH is between GlcN and GlcN Trichoderma longibrachiatum ? - ? 89 3.2.1.91 additional information TTCBH6B is active against shorter oligosaccharides rather than polysaccharides Thermothielavioides terrestris ? - ? 89 3.2.1.91 additional information no activity with cellotriose, alpha-cellulose, cellobiose, and raffinose. The enzyme completely lacks chromogenic activity on the 4-nitrophenyl cellodextrins from 4-nitrophenyl glucoside to 4-nitrophenyl cellobioside Streptomyces coelicolor A(3) ? - ? 89 3.2.1.91 additional information comparison of activities on chitosan by cellobiohydrolases, chitosanases, and lysozyme, oligomer pattern, overview. The different enzymes produce chito-oligosaccharides (COSs) with varying acetylation, NMR spectrometric analysis Trichoderma reesei NRRL 11460 ? - ? 89 3.2.1.91 additional information no substrates: avicel, soluble oat spelt xylan, Pachyman, 4-nitrophenyl-beta-D-cellobioside, 4-nitrophenyl-beta-D-galactopyranoside Orpinomyces sp. Y102 ? - ? 89 3.2.1.91 additional information no activity with 4-methylumbelliferyl beta-D-lactopyranoside or 4-methylumbelliferyl beta-D-cellobioside Aspergillus nidulans ATCC 38163 ? - ? 89 3.2.1.91 additional information no activity with cellotriose, and cellotetraose Fomitopsis pinicola KMJ812 ? - ? 89 3.2.1.91 additional information Cel5A treated cellulose is no longer a substrate for Cel6A or Cel7A Trichoderma reesei QM9414 ? - ? 89 3.2.1.91 additional information comparison of activities on chitosan by cellobiohydrolases, chitosanases, and lysozyme, oligomer pattern, overview. The different enzymes produce chito-oligosaccharides (COSs) with varying acetylation, NMR spectrometric analysis Trichoderma reesei BCRC 32924 ? - ? 89 3.2.1.91 additional information has a multi domain structure composed of a cellulose-binding domain, a Ser/Thr/Pro-rich linker, and a catalytic domain belonging to family 6 of glycosyl hydrolases, from the N-terminus Irpex lacteus MC-2 ? - ? 89 3.2.1.91 additional information no substrates: avicel, 4-methylumbelliferyl beta-D-cellobioside, 4-nitrophenyl beta-D-cellobioside, 4-nitrophenyl beta-D-lactopyranoside Thermothielavioides terrestris ATCC 3808 ? - ? 89 3.2.1.91 additional information does not hydrolyze carboxymethylcellulose Talaromyces purpureogenus KJS506 ? - ? 89 3.2.1.91 additional information comparison of activities on chitosan by cellobiohydrolases, chitosanases, and lysozyme, oligomer pattern, overview. The different enzymes produce chito-oligosaccharides (COSs) with varying acetylation, NMR spectrometric analysis Trichoderma reesei ATCC 56765 ? - ? 89 3.2.1.91 additional information comparison of activities on chitosan by cellobiohydrolases, chitosanases, and lysozyme, oligomer pattern, overview. The different enzymes produce chito-oligosaccharides (COSs) with varying acetylation, NMR spectrometric analysis Trichoderma reesei RUT C-30 ? - ? 89 3.2.1.91 additional information no detectable activity on laminarin, 4-nitrophenyl-beta-D-glucopyranoside, oat spelt xylan, and birch wood xylan Neocallimastix patriciarum J11 ? - ? 89 3.2.1.91 additional information comparison of activities on chitosan by cellobiohydrolases, chitosanases, and lysozyme, oligomer pattern, overview. The different enzymes produce chito-oligosaccharides (COSs) with varying acetylation, NMR spectrometric analysis. The preferred cleavage site of enzyme CBH is between GlcN and GlcN Trichoderma longibrachiatum ATCC 18648 ? - ? 89 3.2.1.91 oat spelt xylan + H2O about 2% activity compared to Avicel Streptomyces coelicolor ? - ? 382438 3.2.1.91 p-nitrophenyl-beta-D-cellobioside + H2O - Niallia circulans p-nitrophenol + cellobiose - ? 259934 3.2.1.91 p-nitrophenyl-beta-D-cellobioside + H2O - Ruminococcus albus p-nitrophenol + cellobiose - ? 259934 3.2.1.91 p-nitrophenyl-beta-D-cellobioside + H2O - Fibrobacter succinogenes p-nitrophenol + cellobiose - ? 259934 3.2.1.91 p-nitrophenyl-beta-D-cellobioside + H2O - Phanerodontia chrysosporium p-nitrophenol + cellobiose - ? 259934 3.2.1.91 p-nitrophenyl-beta-D-cellobioside + H2O - Acetivibrio thermocellus p-nitrophenol + cellobiose - ? 259934 3.2.1.91 p-nitrophenyl-beta-D-cellobioside + H2O - Cellulomonas fimi p-nitrophenol + cellobiose - ? 259934 3.2.1.91 p-nitrophenyl-beta-D-cellobioside + H2O - Ruminococcus flavefaciens p-nitrophenol + cellobiose - ? 259934 3.2.1.91 p-nitrophenyl-beta-D-cellobioside + H2O - Thermotoga sp. p-nitrophenol + cellobiose - ? 259934 3.2.1.91 p-nitrophenyl-beta-D-cellobioside + H2O - Phanerodontia chrysosporium CBH I p-nitrophenol + cellobiose - ? 259934 3.2.1.91 p-nitrophenyl-beta-D-cellobioside + H2O - Thermotoga sp. FjSS3-B.1 p-nitrophenol + cellobiose - ? 259934 3.2.1.91 p-nitrophenyl-beta-D-cellobioside + H2O - Fomitopsis betulina p-nitrophenol + beta-D-cellobiose - ? 374199 3.2.1.91 p-nitrophenyl-beta-D-cellobioside + H2O - Fomitopsis betulina CCBAS585 p-nitrophenol + beta-D-cellobiose - ? 374199 3.2.1.91 p-nitrophenyl-lactoside + H2O - Fibrobacter succinogenes p-nitrophenol + lactose - ? 259923 3.2.1.91 p-nitrophenyl-lactoside + H2O - Thermothelomyces heterothallicus p-nitrophenol + lactose - ? 259923 3.2.1.91 p-nitrophenyl-lactoside + H2O - Acetivibrio thermocellus p-nitrophenol + lactose - ? 259923 3.2.1.91 p-nitrophenyl-lactoside + H2O - Acetivibrio thermocellus CBH III p-nitrophenol + lactose - ? 259923 3.2.1.91 phosphoric acid swollen cellulose + H2O - Trichoderma reesei ? - ? 374331 3.2.1.91 phosphoric acid swollen cellulose + H2O - Thermochaetoides thermophila ? - ? 374331 3.2.1.91 phosphoric acid swollen cellulose + H2O 217% activity compared to cellulose Pyricularia oryzae ? - ? 374331 3.2.1.91 phosphoric acid swollen cellulose + H2O - Thermochaetoides thermophila DSM 1495 ? - ? 374331 3.2.1.91 phosphoric acid swollen cellulose + H2O - Trichoderma reesei QM9414 ? - ? 374331 3.2.1.91 phosphoric acid swollen cellulose + H2O shows 9fold higher activity toward phosphoric acid swollen cellulose than toward avicel Irpex lacteus cellobiose + cellotriose + ? - ? 406400 3.2.1.91 phosphoric acid swollen cellulose + H2O shows 9fold higher activity toward phosphoric acid swollen cellulose than toward avicel Irpex lacteus MC-2 cellobiose + cellotriose + ? - ? 406400 3.2.1.91 phosphoric acid swollen cellulose + H2O - Trichoderma reesei cellobiose + ? - ? 435731 3.2.1.91 phosphoric acid swollen cellulose + H2O - Humicola insolens cellobiose + ? - ? 435731 3.2.1.91 phosphoric acid swollen cellulose + H2O - Thermochaetoides thermophila cellobiose + ? - ? 435731 3.2.1.91 phosphoric acid-swollen Avicel + H2O low specific activity Neocallimastix patriciarum cellobiose - ? 432298 3.2.1.91 phosphoric acid-swollen cellulose + H2O - Thermochaetoides thermophila cellobiose + ? - ? 445791 3.2.1.91 pretreated corn-stover + H2O - Trichoderma reesei ? - ? 435742 3.2.1.91 pretreated rice straw + H2O - Trichoderma reesei ? - ? 435743 3.2.1.91 regenerated amorphous cellulose + H2O On amorphous cellulose, the enzyme is able to initiate hydrolysis on about 4% of the sites to which it could adsorb Trichoderma reesei ? - ? 448088 3.2.1.91 sodium hydroxide pretreated corn stover + H2O - Trichoderma reesei ? - ? 432377 3.2.1.91 sodium hydroxide pretreated corn stover + H2O - Trichoderma reesei ZU-02 ? - ? 432377 3.2.1.91 stachyose + H2O about 18% activity compared to Avicel Streptomyces coelicolor ? - ? 94159 3.2.1.91 stachyose + H2O about 18% activity compared to Avicel Streptomyces coelicolor A(3) ? - ? 94159 3.2.1.91 sulforhodamine-conjugated cellohexaose + H2O - Pyricularia oryzae cellotetraose + cellotriose - ? 417677 3.2.1.91 xylan + H2O 1.13% activity compared to 4-nitrophenyl beta-D-cellobioside Fomitopsis pinicola ? - ? 94170 3.2.1.91 xylan + H2O 1.13% activity compared to 4-nitrophenyl beta-D-cellobioside Fomitopsis pinicola KMJ812 ? - ? 94170 3.2.1.91 xylan + H2O - Niallia circulans xylobiose + xylotetraose + xylohexaose - ? 259940 3.2.1.91 xylan + H2O - Acetivibrio thermocellus xylobiose + xylotetraose + xylohexaose - ? 259940 3.2.1.91 xylan + H2O - Thermoclostridium stercorarium xylobiose + xylotetraose + xylohexaose - ? 259940 3.2.1.91 xyloglucan + H2O low activity Streptomyces coelicolor ? - ? 259816 3.2.1.91 xyloglucan + H2O about 37% activity compared to Avicel Streptomyces coelicolor ? - ? 259816 3.2.1.91 xyloglucan + H2O about 37% activity compared to Avicel Streptomyces coelicolor A(3) ? - ? 259816