3.2.1.86 2,4-dinitrophenyl 6-phospho-beta-D-glucoside + H2O - Thermotoga maritima 2,4-dinitrophenol + D-glucose 6-phosphate - ? 390591 3.2.1.86 2,4-dinitrophenyl 6-phospho-beta-D-glucoside + H2O high activity Oenococcus oeni 2,4-dinitrophenol + D-glucose 6-phosphate - ? 390591 3.2.1.86 2,5-dinitrophenyl 6-phospho-beta-D-glucoside + H2O - Thermotoga maritima 2,5-dinitrophenol + D-glucose 6-phosphate - ? 390600 3.2.1.86 2-nitrophenyl 6-phospho-beta-D-glucoside + H2O - Thermotoga maritima 2-nitrophenol + D-glucose 6-phosphate - ? 390656 3.2.1.86 2-nitrophenyl beta-D-galactoside 6-phosphate + H2O - Escherichia coli 2-nitrophenol + beta-D-galactose 6-phosphate - ? 452838 3.2.1.86 2-nitrophenyl-beta-D-glucopyranoside 6-phosphate + H2O - Lactobacillus gasseri 2-nitrophenol + beta-D-glucose 6-phosphate - ? 431327 3.2.1.86 2-nitrophenyl-beta-D-glucopyranoside 6-phosphate + H2O - Lactobacillus gasseri ATCC 33323 2-nitrophenol + beta-D-glucose 6-phosphate - ? 431327 3.2.1.86 3,4-dinitrophenyl 6-phospho-beta-D-glucoside + H2O - Thermotoga maritima 3,4-dinitrophenol + D-glucose 6-phosphate - ? 390715 3.2.1.86 3,5-dichlorophenyl 6-phospho-beta-D-glucoside + H2O - Thermotoga maritima 3,5-dichlorophenol + D-glucose 6-phosphate - ? 390718 3.2.1.86 3-nitrophenyl 6-phospho-beta-D-glucoside + H2O - Thermotoga maritima 3-nitrophenol + D-glucose 6-phosphate - ? 390757 3.2.1.86 4-chloro-2-nitrophenyl 6-phospho-beta-D-glucoside + H2O - Thermotoga maritima 4-chloro-2-nitrophenol + D-glucose 6-phosphate - ? 390795 3.2.1.86 4-cyanophenyl 6-phospho-beta-D-glucoside + H2O - Thermotoga maritima 4-cyanophenol + D-glucose 6-phosphate - ? 390799 3.2.1.86 4-methyl-2-oxo-2H-chromen-7-yl 6-O-phosphono-beta-D-glucopyranoside + H2O - Escherichia coli ? - ? 452837 3.2.1.86 4-methylumbelliferyl-beta-D-glucopyranoside 6-phosphate + H2O - Fusobacterium mortiferum 4-methylumbelliferone + D-glucose 6-phosphate - ? 94732 3.2.1.86 4-methylumbelliferyl-beta-D-glucoside + H2O - Pectobacterium carotovorum 4-methylumbelliferone + D-glucose - ? 381161 3.2.1.86 4-nitrophenyl 6-phospho-beta-D-glucoside + H2O - Thermotoga maritima 4-nitrophenol + D-glucose 6-phosphate - ? 390912 3.2.1.86 4-nitrophenyl 6-phospho-beta-glucoside + H2O - Streptococcus pneumoniae 4-nitrophenol + D-glucose 6-phosphate - ? 446063 3.2.1.86 4-nitrophenyl 6-phospho-beta-glucoside + H2O - Streptococcus pyogenes 4-nitrophenol + D-glucose 6-phosphate - ? 446063 3.2.1.86 4-nitrophenyl 6-phospho-beta-glucoside + H2O - Streptococcus pyogenes M1 4-nitrophenol + D-glucose 6-phosphate - ? 446063 3.2.1.86 4-nitrophenyl beta-D-glucopyranoside + H2O strains expressing the Bgl isoform Val204 show a lower hydrolysing activity on 4-nitrophenyl-beta-D-glucopyranoside when compared to strains that possess the Bgl isoform Ala204 Mycoplasma mycoides 4-nitrophenol + D-glucopyranose - ? 94269 3.2.1.86 4-nitrophenyl beta-D-glucopyranoside 6-phosphate + H2O - Escherichia coli 4-nitrophenol + beta-D-glucopyranose 6-phosphate - ? 431406 3.2.1.86 4-nitrophenyl beta-D-glucopyranoside 6-phosphate + H2O - uncultured organism 4-nitrophenol + beta-D-glucopyranose 6-phosphate - ? 431406 3.2.1.86 4-nitrophenyl beta-D-glucopyranoside 6-phosphate + H2O - Streptococcus pyogenes 4-nitrophenol + beta-D-glucose 6-phosphate - ? 431407 3.2.1.86 4-nitrophenyl beta-D-glucopyranoside 6-phosphate + H2O - Streptococcus pneumoniae 4-nitrophenol + beta-D-glucose 6-phosphate - ? 431407 3.2.1.86 4-nitrophenyl beta-D-glucopyranoside 6-phosphate + H2O - Streptococcus pyogenes M1 GAS SF370 4-nitrophenol + beta-D-glucose 6-phosphate - ? 431407 3.2.1.86 4-nitrothiophenyl beta-D-glucoside 6-phosphate + H2O - Escherichia coli 4-nitrothiophenol + beta-D-glucose 6-phosphate - ? 452839 3.2.1.86 4-tert-butylphenyl 6-phospho-beta-D-glucoside + H2O - Thermotoga maritima 4-tert-butylphenol + D-glucose 6-phosphate - ? 390963 3.2.1.86 6-phospho-beta-D-glucosyl-(1->4)-D-glucose + H2O - Oenococcus oeni D-glucose + D-glucose 6-phosphate - ? 446074 3.2.1.86 6-phospho-beta-D-glucosyl-(1->4)-D-glucose + H2O - Escherichia coli D-glucose + D-glucose 6-phosphate - ? 446074 3.2.1.86 arbutin + H2O - Escherichia coli ? - ? 94661 3.2.1.86 arbutin 6-phosphate + H2O - Escherichia coli hydroquinone + D-glucose 6-phosphate - ? 94723 3.2.1.86 arbutin 6-phosphate + H2O - Klebsiella aerogenes hydroquinone + D-glucose 6-phosphate - ? 94723 3.2.1.86 arbutin 6-phosphate + H2O - Fusobacterium mortiferum hydroquinone + D-glucose 6-phosphate - ? 94723 3.2.1.86 cellobiitol monophosphate + H2O - Klebsiella aerogenes ? - ? 94726 3.2.1.86 cellobiose 6-phosphate + H2O - Klebsiella aerogenes D-glucose 6-phosphate + D-glucose - ? 94724 3.2.1.86 cellobiose 6-phosphate + H2O - Fusobacterium mortiferum D-glucose 6-phosphate + D-glucose - ? 94724 3.2.1.86 cellobiose 6-phosphate + H2O - Escherichia coli D-glucose 6-phosphate + D-glucose - ? 94724 3.2.1.86 cellobiose 6-phosphate + H2O - Thermotoga maritima D-glucose 6-phosphate + D-glucose - ? 94724 3.2.1.86 cellobiose 6-phosphate + H2O - Streptococcus pneumoniae beta-D-glucose + glucose 6-phosphate - ? 430924 3.2.1.86 esculin + H2O - Escherichia coli ? - ? 94672 3.2.1.86 esculin 6-phosphate + H2O - Fusobacterium mortiferum ? - ? 94736 3.2.1.86 gentiobiose 6-phosphate + H2O - Klebsiella aerogenes D-glucose 6-phosphate + D-glucose - ? 94725 3.2.1.86 gentiobiose 6-phosphate + H2O - Fusobacterium mortiferum D-glucose 6-phosphate + D-glucose - ? 94725 3.2.1.86 gentiobiose 6-phosphate + H2O - Escherichia coli D-glucose 6-phosphate + D-glucose - ? 94725 3.2.1.86 lactose 6-phosphate + H2O - Geobacillus stearothermophilus beta-D-galactose-6-phosphate + D-glucose - ? 447676 3.2.1.86 lactose 6-phosphate + H2O - Geobacillus stearothermophilus T-1 beta-D-galactose-6-phosphate + D-glucose - ? 447676 3.2.1.86 laminaribiose 6-phosphate + H2O - Fusobacterium mortiferum D-glucose 6-phosphate + D-glucose - ? 94734 3.2.1.86 methyl beta-D-glucoside monophosphate + H2O - Klebsiella aerogenes ? - ? 94728 3.2.1.86 methyl beta-D-glucoside monophosphate + H2O - Fusobacterium mortiferum ? - ? 94728 3.2.1.86 methyl beta-glucoside 6-phosphate + H2O - Escherichia coli ? - ? 94730 3.2.1.86 methyl beta-glucoside 6-phosphate + H2O - Fusobacterium mortiferum ? - ? 94730 3.2.1.86 additional information expression of P-beta-glc induced by lactose is characteristic of Lactobacillus gasseri. Because this lactic acid bacterium is a major inhabitant of the human intestine, this enzyme is a key glycosidase involved in lactose utilization Lactobacillus gasseri ? - ? 89 3.2.1.86 additional information single nucleotide polymorphism in the bgl gene coding for the 6-phospho-beta-glucosidase is one possible cause of the difference in bacterial virulence among strains of Mycoplasma mycoides subsp. mycoides SC. Bgl does not act as a direct virulence factor, but strains possessing the Bgl isoform Val204 with low hydrolysing activity are more prone to survive in environments that contain high levels of beta-D-glucosides, thus contributing in some extent to mycoplasmaemia Mycoplasma mycoides ? - ? 89 3.2.1.86 additional information an essential cysteine residue is present at the active site. Members of GH4 employ a unique catalytic mechanism for cleavage of the glycosidic bond. GH4 single or double-displacement mechanism Erwinia rhapontici ? - ? 89 3.2.1.86 additional information the enzyme is not active against non-phosphorylated beta-glucosides Oenococcus oeni ? - ? 89 3.2.1.86 additional information no activity with beta-(1,4)-thiocellobiose 6-phosphate Streptococcus pneumoniae ? - ? 89 3.2.1.86 additional information the enzyme also shows 6-phospho-beta-galactosidase activity, EC 3.2.1.85 Lactobacillus gasseri ? - ? 89 3.2.1.86 additional information the enzyme shows no beta-glucosidase activity, EC 3.2.1.21 uncultured organism ? - ? 89 3.2.1.86 additional information the enzyme SPy1599 contains a phosphate-binding pocket in the active site and has specific 6-phospho-beta-glucosidase activity. No or very poor activity with 4-nitrophenyl derivatives of galactose, glucose, xylose, galactose 6-phosphate, N-acetyl-D-galactosamine, and N-acetyl-D-glusosamine Streptococcus pyogenes ? - ? 89 3.2.1.86 additional information no activity with 2-nitrophenyl beta-D-galactopyranoside, 4-methylumbelliferyl N-acetyl-alpha-D-glucosaminide, 4-methylumbelliferyl beta-D-glucopyranoside, 4-methylumbelliferyl alpha-D-glucopyranoside, 2-nitrophenyl alpha-D-galactopyranoside, 4-nitrophenyl alpha-D-glucoside 6-phosphate, 4-nitrophenyl alpha-D-mannoside 6-phosphate, and 4-nitrophenyl alpha-D-galactoside 6-phosphate Streptococcus pneumoniae ? - ? 89 3.2.1.86 additional information the enzyme also shows 6-phospho-beta-galactosidase activity, EC 3.2.1.85 Lactobacillus gasseri ATCC 33323 ? - ? 89 3.2.1.86 additional information the enzyme SPy1599 contains a phosphate-binding pocket in the active site and has specific 6-phospho-beta-glucosidase activity. No or very poor activity with 4-nitrophenyl derivatives of galactose, glucose, xylose, galactose 6-phosphate, N-acetyl-D-galactosamine, and N-acetyl-D-glusosamine Streptococcus pyogenes M1 GAS SF370 ? - ? 89 3.2.1.86 additional information an essential cysteine residue is present at the active site. Members of GH4 employ a unique catalytic mechanism for cleavage of the glycosidic bond. GH4 single or double-displacement mechanism Erwinia rhapontici DSM 448 ? - ? 89 3.2.1.86 o-nitrophenyl-beta-D-galactopyranoside 6-phosphate + H2O - Escherichia coli o-nitrophenol + D-galactose 6-phosphate - ? 94737 3.2.1.86 o-nitrophenyl-beta-D-galactopyranoside 6-phosphate + H2O - Lactococcus lactis o-nitrophenol + D-galactose 6-phosphate - ? 94737 3.2.1.86 o-nitrophenyl-beta-D-galactopyranoside 6-phosphate + H2O - Lactobacillus delbrueckii o-nitrophenol + D-galactose 6-phosphate - ? 94737 3.2.1.86 o-nitrophenyl-beta-D-galactose + H2O very low activity Lactobacillus delbrueckii o-nitrophenol + D-galactose - ? 374074 3.2.1.86 p-nitrophenyl 6-phospho-beta-D-thioglucoside + H2O - Thermotoga maritima p-nitrophenol + 6-phospho-beta-D-thioglucose - ? 382458 3.2.1.86 p-nitrophenyl-beta-D-glucopyranoside + H2O - Pectobacterium carotovorum p-nitrophenol + D-glucose - ? 382492 3.2.1.86 p-nitrophenyl-beta-D-glucopyranoside 6-phosphate + H2O - Escherichia coli p-nitrophenol + D-glucopyranose 6-phosphate - ? 94731 3.2.1.86 p-nitrophenyl-beta-D-glucopyranoside 6-phosphate + H2O - Fusobacterium mortiferum p-nitrophenol + D-glucopyranose 6-phosphate - ? 94731 3.2.1.86 p-nitrophenyl-beta-D-glucopyranoside 6-phosphate + H2O - Thermotoga maritima p-nitrophenol + D-glucopyranose 6-phosphate - ? 94731 3.2.1.86 p-nitrophenyl-beta-D-glucopyranoside 6-phosphate + H2O isoenzyme B Escherichia coli p-nitrophenol + D-glucopyranose 6-phosphate - ? 94731 3.2.1.86 p-nitrophenyl-beta-D-glucopyranoside 6-phosphate + H2O - Lactobacillus delbrueckii p-nitrophenol + D-glucose 6-phosphate - ? 374217 3.2.1.86 p-nitrophenyl-beta-D-glucopyranoside 6-phosphate + H2O - Thermotoga maritima p-nitrophenol + beta-D-glucopyranose 6-phosphate - ? 382493 3.2.1.86 p-nitrophenyl-beta-D-glucopyranoside-6-phosphate + H2O - Pectobacterium carotovorum p-nitrophenol + D-glucose 6-phosphate - ? 382494 3.2.1.86 p-nitrophenyl-beta-D-glucose + H2O very low activity Lactobacillus delbrueckii p-nitrophenol + D-glucose - ? 374219 3.2.1.86 p-nitrothiophenyl beta-glucoside 6-phosphate + H2O - Escherichia coli ? - ? 94729 3.2.1.86 phenyl beta-D-glucoside 6-phosphate + H2O - Escherichia coli phenol + glucose 6-phosphate - ? 94727 3.2.1.86 phenyl beta-D-glucoside 6-phosphate + H2O - Klebsiella aerogenes phenol + glucose 6-phosphate - ? 94727 3.2.1.86 salicin + H2O - Escherichia coli ? - ? 13703 3.2.1.86 salicin 6-phosphate + H2O - Klebsiella aerogenes 2-hydroxymethylphenol + D-glucose 6-phosphate - ? 94722 3.2.1.86 salicin 6-phosphate + H2O - Fusobacterium mortiferum 2-hydroxymethylphenol + D-glucose 6-phosphate - ? 94722 3.2.1.86 salicin 6-phosphate + H2O - Escherichia coli 2-hydroxymethylphenol + D-glucose 6-phosphate - ? 94722 3.2.1.86 salicin 6-phosphate + H2O isoenzyme B Escherichia coli 2-hydroxymethylphenol + D-glucose 6-phosphate - ? 94722 3.2.1.86 sophorose 6-phosphate + H2O - Fusobacterium mortiferum D-glucose 6-phosphate + D-glucose - ? 94733