3.2.1.35 4-S-chondroitin sulfate + H2O - Apis mellifera ? - ? 399705 3.2.1.35 4-S-chondroitin sulfate + H2O - synthetic construct ? - ? 399705 3.2.1.35 4-S-chondroitin sulfate + H2O - Homo sapiens ? - ? 399705 3.2.1.35 6-S-chondroitin sulfate + H2O - Homo sapiens ? - ? 399766 3.2.1.35 6-S-chondroitin sulfate + H2O the enzyme is capable of degrading chondroitin sulfate C at a rate of around 40% compared to its hyaluronidase activity Apis mellifera ? - ? 399766 3.2.1.35 6-S-chondroitin sulfate + H2O the enzyme is capable of degrading CS-C at a rate of around 40% compared to its hyaluronidase activity synthetic construct ? - ? 399766 3.2.1.35 chitin + H2O - Bos taurus ? - ? 115729 3.2.1.35 chitin + H2O 12 h incubation Nephrops norvegicus ? - ? 115729 3.2.1.35 chondroitin + H2O - Bos taurus ? - ? 391656 3.2.1.35 chondroitin + H2O - Ovis aries ? - ? 391656 3.2.1.35 chondroitin + H2O - Caenorhabditis elegans GlcAbeta(1-3)GalNAcbeta(1-4)GlcAbeta(1-3)GalNAc + ? main product ir 400669 3.2.1.35 chondroitin + H2O - Caenorhabditis elegans N2 GlcAbeta(1-3)GalNAcbeta(1-4)GlcAbeta(1-3)GalNAc + ? main product ir 400669 3.2.1.35 chondroitin 4-sulfate + H2O - Bos taurus ? - ? 391657 3.2.1.35 chondroitin 4-sulfate + H2O - Crotalus durissus terrificus ? - ? 391657 3.2.1.35 chondroitin 4-sulfate + H2O 28% activity compared to sodium hyaluronate Bos grunniens ? - ? 391657 3.2.1.35 chondroitin 6-sulfate + H2O - Bos taurus ? - ? 404663 3.2.1.35 chondroitin 6-sulfate + H2O - Crotalus durissus terrificus ? - ? 404663 3.2.1.35 chondroitin 6-sulfate + H2O 19% activity compared to sodium hyaluronate Bos grunniens ? - ? 404663 3.2.1.35 chondroitin B sulfate + H2O very little hydrolase activity towards chondroitin sulfate B (dermatan sulfate) Apis mellifera ? - ? 391658 3.2.1.35 chondroitin sulfate + H2O - Homo sapiens ? - ? 391659 3.2.1.35 chondroitin sulfate + H2O - Bos taurus ? - ? 391659 3.2.1.35 chondroitin sulfate + H2O - Ovis aries ? - ? 391659 3.2.1.35 chondroitin sulfate + H2O - Caenorhabditis elegans ? - ? 391659 3.2.1.35 chondroitin sulfate + H2O - Culex quinquefasciatus ? - ? 391659 3.2.1.35 chondroitin sulfate + H2O - Ctenocephalides felis ? - ? 391659 3.2.1.35 chondroitin sulfate + H2O - Phlebotomus papatasi ? - ? 391659 3.2.1.35 chondroitin sulfate + H2O - Eusimulium latipes ? - ? 391659 3.2.1.35 chondroitin sulfate + H2O - Chrysops viduatus ? - ? 391659 3.2.1.35 chondroitin sulfate + H2O - Simulium ornatum ? - ? 391659 3.2.1.35 chondroitin sulfate + H2O - Culicoides kibunensis ? - ? 391659 3.2.1.35 chondroitin sulfate + H2O - Loxosceles intermedia ? - ? 391659 3.2.1.35 chondroitin sulfate + H2O - Caenorhabditis elegans N2 ? - ? 391659 3.2.1.35 chondroitin sulfate A + H2O - Bos taurus oligosaccharides + ? - ? 115731 3.2.1.35 chondroitin sulfate A + H2O - Loxosceles intermedia oligosaccharides + ? - ? 115731 3.2.1.35 chondroitin sulfate A + H2O acting at the same rate as with hyaluronic acid Bos taurus oligosaccharides + ? - ? 115731 3.2.1.35 chondroitin sulfate A + H2O production of even-numbered oligosaccharides only produced by lysosomal hyaluronidase Canis lupus familiaris oligosaccharides + ? - ? 115731 3.2.1.35 chondroitin sulfate A + H2O - Mus musculus ? - ? 391660 3.2.1.35 chondroitin sulfate A + H2O - Apis mellifera ? - ? 391660 3.2.1.35 chondroitin sulfate A + H2O poor substrate Homo sapiens ? - ? 391660 3.2.1.35 chondroitin sulfate B + H2O very little hydrolase activity Apis mellifera ? - ? 400670 3.2.1.35 chondroitin sulfate B + H2O very little hydrolase activity synthetic construct ? - ? 400670 3.2.1.35 chondroitin sulfate C + H2O - Mus musculus ? - ? 115732 3.2.1.35 chondroitin sulfate C + H2O - Homo sapiens ? - ? 115732 3.2.1.35 chondroitin sulfate C + H2O - Apis mellifera ? - ? 115732 3.2.1.35 chondroitin sulfate C + H2O acting at the same rate as with hyaluronic acid Bos taurus ? - ? 115732 3.2.1.35 chondroitin sulfate D + H2O - Mus musculus ? - ? 430057 3.2.1.35 chondroitin sulfate D + H2O - Homo sapiens ? - ? 430057 3.2.1.35 chondroitin sulfate-rich proteoglycan + H2O from bovine nasal cartilage Bothrops asper ? - ? 364718 3.2.1.35 chondroitin sulfate-rich proteoglycan + H2O from bovine nasal cartilage Protobothrops flavoviridis ? - ? 364718 3.2.1.35 chondroitin sulfate-rich proteoglycan + H2O from bovine nasal cartilage Crotalus atrox ? - ? 364718 3.2.1.35 chondroitin sulfate-rich proteoglycan + H2O from bovine nasal cartilage Daboia russelii ? - ? 364718 3.2.1.35 chondroitin sulfate-rich proteoglycan + H2O from bovine nasal cartilage Protobothrops mucrosquamatus ? - ? 364718 3.2.1.35 chondroitin sulfate-rich proteoglycan + H2O from bovine nasal cartilage Gloydius blomhoffii ? - ? 364718 3.2.1.35 chondroitin sulfate-rich proteoglycan + H2O from bovine nasal cartilage Naja naja ? - ? 364718 3.2.1.35 chondroitin sulfate-rich proteoglycan + H2O from bovine nasal cartilage Echis carinatus ? - ? 364718 3.2.1.35 chondroitin sulfate-rich proteoglycan + H2O from bovine nasal cartilage Naja melanoleuca ? - ? 364718 3.2.1.35 chondroitin sulfate-rich proteoglycan + H2O from bovine nasal cartilage Naja kaouthia ? - ? 364718 3.2.1.35 chondroitin sulfate-rich proteoglycan + H2O from bovine nasal cartilage Naja sputatrix ? - ? 364718 3.2.1.35 chondroitin sulfate-rich proteoglycan + H2O from bovine nasal cartilage Vipera elegans ? - ? 364718 3.2.1.35 chondroitin sulfate-rich proteoglycan + H2O from bovine nasal cartilage Echis pyramidum leakeyi ? - ? 364718 3.2.1.35 chondroitin sulfate-rich proteoglycan + H2O from bovine nasal cartilage Echis carinatus sochureki ? - ? 364718 3.2.1.35 chondroitin sulfate-rich proteoglycan + H2O from bovine nasal cartilage Protobothrops elegans ? - ? 364718 3.2.1.35 Collagen + H2O - Mus musculus ? - ? 16347 3.2.1.35 dermatan + H2O - Crotalus durissus terrificus ? - ? 430099 3.2.1.35 dermatan + H2O 7% activity compared to sodium hyaluronate Bos grunniens ? - ? 430099 3.2.1.35 hyaluronan + 2 H2O - Mus musculus N-acetyl-beta-D-glucosamine + D-glucuronate + ? - ? 364711 3.2.1.35 hyaluronan + 2 H2O - Homo sapiens N-acetyl-beta-D-glucosamine + D-glucuronate + ? - ? 364711 3.2.1.35 hyaluronan + 2 H2O - Rattus norvegicus N-acetyl-beta-D-glucosamine + D-glucuronate + ? - ? 364711 3.2.1.35 hyaluronan + 2 H2O - Sus scrofa N-acetyl-beta-D-glucosamine + D-glucuronate + ? - ? 364711 3.2.1.35 hyaluronan + 2 H2O - Bos taurus N-acetyl-beta-D-glucosamine + D-glucuronate + ? - ? 364711 3.2.1.35 hyaluronan + 2 H2O - Ovis aries N-acetyl-beta-D-glucosamine + D-glucuronate + ? - ? 364711 3.2.1.35 hyaluronan + 2 H2O - Streptococcus agalactiae N-acetyl-beta-D-glucosamine + D-glucuronate + ? - ? 364711 3.2.1.35 hyaluronan + 2 H2O - Apis mellifera N-acetyl-beta-D-glucosamine + D-glucuronate + ? - ? 364711 3.2.1.35 hyaluronan + 2 H2O from Streptococcus zooepidemicus Homo sapiens N-acetyl-beta-D-glucosamine + D-glucuronate + ? - ? 364711 3.2.1.35 hyaluronan + 2 H2O from Streptococcus zooepidemicus, degradation Homo sapiens N-acetyl-beta-D-glucosamine + D-glucuronate + ? - ? 364711 3.2.1.35 hyaluronan + 2 H2O from Streptococcus zooepidemicus, degradation Bos taurus N-acetyl-beta-D-glucosamine + D-glucuronate + ? - ? 364711 3.2.1.35 hyaluronan + 2 H2O from Streptococcus zooepidemicus, degradation Oryctolagus cuniculus N-acetyl-beta-D-glucosamine + D-glucuronate + ? - ? 364711 3.2.1.35 hyaluronan + 2 H2O absolute specificity for hyaluronan Hippasa partita N-acetyl-beta-D-glucosamine + D-glucuronate + ? - ? 364711 3.2.1.35 hyaluronan + 2 H2O highly specific substrate with 10fold higher activity compared to chondroitin 4-sulfate, chondroitin 6-sulfate, or dermatan Crotalus durissus terrificus N-acetyl-beta-D-glucosamine + D-glucuronate + ? - ? 364711 3.2.1.35 hyaluronan + 2 H2O - Streptococcus agalactiae GD201008-001 N-acetyl-beta-D-glucosamine + D-glucuronate + ? - ? 364711 3.2.1.35 hyaluronan + H2O - Bos taurus tetraoligosaccharides + hexaoligosaccharides - r 115727 3.2.1.35 hyaluronan + H2O - Homo sapiens tetraoligosaccharides + hexaoligosaccharides after complete digestion ? 115727 3.2.1.35 hyaluronan + H2O - Mus musculus ? - ? 364708 3.2.1.35 hyaluronan + H2O - Homo sapiens ? - ? 364708 3.2.1.35 hyaluronan + H2O - Bos taurus ? - ? 364708 3.2.1.35 hyaluronan + H2O - Culex quinquefasciatus ? - ? 364708 3.2.1.35 hyaluronan + H2O - Ctenocephalides felis ? - ? 364708 3.2.1.35 hyaluronan + H2O - Phlebotomus papatasi ? - ? 364708 3.2.1.35 hyaluronan + H2O - Eusimulium latipes ? - ? 364708 3.2.1.35 hyaluronan + H2O - Chrysops viduatus ? - ? 364708 3.2.1.35 hyaluronan + H2O - Simulium ornatum ? - ? 364708 3.2.1.35 hyaluronan + H2O - Potamotrygon motoro ? - ? 364708 3.2.1.35 hyaluronan + H2O - Mesobuthus martensii ? - ? 364708 3.2.1.35 hyaluronan + H2O - Culicoides kibunensis ? - ? 364708 3.2.1.35 hyaluronan + H2O - Synanceia verrucosa ? - ? 364708 3.2.1.35 hyaluronan + H2O - Bothrops atrox ? - ? 364708 3.2.1.35 hyaluronan + H2O - Talaromyces purpureogenus ? catalyzes the endolytic hydrolysis of beta-1,4 glycosidic linkages in hyaluronan. Products are saturated hyaluronan-disaccharides ? 364708 3.2.1.35 hyaluronan + H2O complete degradation, absolutely specific for Xenopus laevis ? - ? 364708 3.2.1.35 hyaluronan + H2O complete degradation, absolutely specific for Homo sapiens ? - ? 364708 3.2.1.35 hyaluronan + H2O wild-type isozyme HYAL1 sequence, amino acid residues 301-330, is critical for hyaluronidase activity Homo sapiens ? - ? 364708 3.2.1.35 hyaluronan + H2O a fluorescent substrate (FRET-HA) to quantitatively assess hyaluronidase activity is developed. Hyaluronan (HA)is dual labeled with fluorescein amine and rhodamine B amine. The fluorescein amine fluorescence signal is significantly quenched and the rhodamine B amine signal is significantly enhanced due to fluorescence resonance energy transfer (FRET). In the presence of bovine testes hyaluronidase, cleavage of HA disrupts FRET, resulting in a loss of the fluorescein amine quenching that is dependent on both enzyme concentration and time. Increase in the fluorescein amine signal can be conveniently monitored in both noncontinuous and continuous fashions Bos taurus ? - ? 364708 3.2.1.35 hyaluronan + H2O HPLC and MS analyses of the products reveal the existance of oligosaccharides which are not produced under hydrolysis conditions, but during transglycosylation by the enzyme, suggesting that a novel enzyme is present as a contaminant in commercial BTH, which acts as an N-deacetylase of N-acetylglucosamine at the reducing terminal of hyaluronan oligosaccharides Bos taurus ? - ? 364708 3.2.1.35 hyaluronan + H2O hyaluronan deposition and turnover is even more abundant and more rapid in malignant tissues. The proportion of low molecular weight (LMW) fragments of HA is greater in tumors and tumor patients than in the normal Homo sapiens ? - ? 364708 3.2.1.35 hyaluronan + H2O hyaluronan fragments are used with a molar mass ranging from 800 g/mol to 250000 g/mol and native hyaluronan to study the influence of the chain length of hyaluronan on the kinetics of its HAase-catalyzed hydrolysis. The initial hydrolysis rate strongly varies with hyaluronan chain length. The ability of HA chains to form an efficient enzyme-substrate complex is maximum for hyaluronan molar masses ranging from 3000 to 20000 g/mol. Shorter hyaluronan chains are too short to form a stable complex and longer hyaluronan chains encounter difficulties in forming a complex, due to steric hindrance. As the chain length decreases the HAase increasingly catalyses transglycosylation rather than hydrolysis. Two hyaluronan chain populations, corresponding to hyaluronan chain molar masses lower and higher than approximately 20000 g/mol are identified and related to the bi-exponential character Bos taurus ? - ? 364708 3.2.1.35 hyaluronan + H2O human platelets degrade the proinflammatory matrix hyaluronan through the activity of HYAL2 and platelet activation causes the immediate translocation of HYAL2 from a distinct population of alpha-granules to platelet surfaces where it exerts its catalytic activity Homo sapiens ? - ? 364708 3.2.1.35 hyaluronan + H2O complete degradation, absolutely specific for Mus musculus N-acetylglucosamine + D-glucuronate + ? - ? 364710 3.2.1.35 hyaluronan + H2O absolutely specific for Agkistrodon contortrix contortrix hexasaccharides + tetrasaccharides main products, with N-acetyl-beta-D-glucosamine at the reducing end, cleavage of beta-1,4-glycosidic linkage, not beta-1,3-glycosidic linkage ? 364714 3.2.1.35 hyaluronan + H2O specific cleavage of beta-1,4-glucosidic linkages Bos taurus oligosaccharides - ? 364715 3.2.1.35 hyaluronan + H2O - Mus musculus hyaluronan oligomers - ? 380623 3.2.1.35 hyaluronan + H2O - Homo sapiens hyaluronan oligomers - ? 380623 3.2.1.35 hyaluronan + H2O - Bos taurus hyaluronan oligomers - ? 380623 3.2.1.35 hyaluronan + H2O - Palamneus gravimanus hyaluronan oligomers - ? 380623 3.2.1.35 hyaluronan + H2O - Apis mellifera hyaluronan oligomers - ? 380623 3.2.1.35 hyaluronan + H2O human substrate Synanceia horrida hyaluronan oligomers - ? 380623 3.2.1.35 hyaluronan + H2O human skin sections Naja naja hyaluronan oligomers - ? 380623 3.2.1.35 hyaluronan + H2O the enzyme is required for fertility facilitating the penetration of sperm through the cumulus mass in the ovum Homo sapiens hyaluronan oligomers - ? 380623 3.2.1.35 hyaluronan + H2O isozyme NNH2 shows endoglycosidase activity, absolute specific for in vitro, substrate from human skin Naja naja hyaluronan oligomers - ? 380623 3.2.1.35 hyaluronan + H2O the enzyme shows low activity, degradation jaagsiekte sheep retrovirus hyaluronan oligomers - ? 380623 3.2.1.35 hyaluronan + H2O absolutely specific for Naja naja tetrasaccharides units of hyaluronan - ? 380624 3.2.1.35 hyaluronan + H2O absolutely specific for, cleavage of beta-1,4-glycosidic linkages, of human skin, extracellular matrix Naja naja tetrasaccharides units of hyaluronan - ? 380624 3.2.1.35 hyaluronan decasaccharide + H2O - Bos taurus hyaluronan tetrasaccharide + hyaluronan hexasaccharide + hyaluronan octasaccharide + 31.5% hyaluronan tetrasaccharide, 48.3% hyaluronan hexasaccharide, 14.7% hyaluronan octasaccharide, 5.5% hyaluronan decasaccharide ? 417093 3.2.1.35 hyaluronan octasaccharide + H2O - Bos taurus hyaluronan tetrasaccharide + hyaluronan hexasaccharide 46.8% hyaluronan tetrasaccharide, 32.7% hyaluronan hexasaccharide, 20.5% hyaluronan octasaccharide. Enzyme generates a disaccharide intermediate from hyaluronan oligosaccharide, the majority of which is transferred to the nonreducing ends of other oligosaccharides, only traces being released as free disaccharide. When hyaluronan octasaccharide, with an unsaturated glucuronic acid at the nonreducing end, is used as a substrate, only a tetrasaccharide product is detected by HPLC. MS shows that the product is a mixture of equal amounts of two tetrasaccharides, one with and the other without the unsaturated glucuronic acid. This suggests that, in the case of substrates with a double bond at the nonreducing end, a tetrasaccharide is cleaved off instead of a disaccharide ? 417094 3.2.1.35 hyaluronan oligosaccharides + H2O specific cleavage of beta-1,4-glucosidic linkages Bos taurus N-acetyl-beta-D-glucosamine + D-glucuronate - ? 364716 3.2.1.35 hyaluronate + H2O - Bos taurus N-acetyl-beta-D-glucosamine + D-glucuronate + ? - ? 364713 3.2.1.35 hyaluronate + H2O - Apis mellifera N-acetyl-beta-D-glucosamine + D-glucuronate + ? - ? 364713 3.2.1.35 hyaluronate + H2O from umbilical cord Bos taurus N-acetyl-beta-D-glucosamine + D-glucuronate + ? - ? 364713 3.2.1.35 hyaluronate + H2O from umbilical cord Homo sapiens N-acetyl-beta-D-glucosamine + D-glucuronate + ? - ? 364713 3.2.1.35 hyaluronate + H2O from human umbilical cord Bos taurus oligosaccharides establishing of a rapid, sensitive PAGE method for analysis of hyaluronan oligosaccharides ? 364717 3.2.1.35 hyaluronic acid + H2O - Cavia porcellus ? - ? 94227 3.2.1.35 hyaluronic acid + H2O - Homo sapiens ? - ? 94227 3.2.1.35 hyaluronic acid + H2O - Sus scrofa ? - ? 94227 3.2.1.35 hyaluronic acid + H2O - Bos taurus ? - ? 94227 3.2.1.35 hyaluronic acid + H2O - Ovis aries ? - ? 94227 3.2.1.35 hyaluronic acid + H2O - Canis lupus familiaris ? - ? 94227 3.2.1.35 hyaluronic acid + H2O - Caenorhabditis elegans ? - ? 94227 3.2.1.35 hyaluronic acid + H2O - Apis mellifera ? - ? 94227 3.2.1.35 hyaluronic acid + H2O - Capra hircus ? - ? 94227 3.2.1.35 hyaluronic acid + H2O - Echis carinatus ? - ? 94227 3.2.1.35 hyaluronic acid + H2O - Streptococcus dysgalactiae ? - ? 94227 3.2.1.35 hyaluronic acid + H2O - Bubalus sp. ? - ? 94227 3.2.1.35 hyaluronic acid + H2O - Loxosceles intermedia ? - ? 94227 3.2.1.35 hyaluronic acid + H2O - Bungarus caeruleus ? - ? 94227 3.2.1.35 hyaluronic acid + H2O - Mus musculus ? - ? 94227 3.2.1.35 hyaluronic acid + H2O - Tityus serrulatus ? - ? 94227 3.2.1.35 hyaluronic acid + H2O degradation in human skin, human muscle, chick embryo Bothrops asper ? - ? 94227 3.2.1.35 hyaluronic acid + H2O degradation in human skin, human muscle, chick embryo Protobothrops flavoviridis ? - ? 94227 3.2.1.35 hyaluronic acid + H2O degradation in human skin, human muscle, chick embryo Crotalus atrox ? - ? 94227 3.2.1.35 hyaluronic acid + H2O degradation in human skin, human muscle, chick embryo Daboia russelii ? - ? 94227 3.2.1.35 hyaluronic acid + H2O degradation in human skin, human muscle, chick embryo Protobothrops mucrosquamatus ? - ? 94227 3.2.1.35 hyaluronic acid + H2O degradation in human skin, human muscle, chick embryo Gloydius blomhoffii ? - ? 94227 3.2.1.35 hyaluronic acid + H2O degradation in human skin, human muscle, chick embryo Naja naja ? - ? 94227 3.2.1.35 hyaluronic acid + H2O degradation in human skin, human muscle, chick embryo Echis carinatus ? - ? 94227 3.2.1.35 hyaluronic acid + H2O degradation in human skin, human muscle, chick embryo Naja melanoleuca ? - ? 94227 3.2.1.35 hyaluronic acid + H2O degradation in human skin, human muscle, chick embryo Naja kaouthia ? - ? 94227 3.2.1.35 hyaluronic acid + H2O degradation in human skin, human muscle, chick embryo Naja sputatrix ? - ? 94227 3.2.1.35 hyaluronic acid + H2O degradation in human skin, human muscle, chick embryo Vipera elegans ? - ? 94227 3.2.1.35 hyaluronic acid + H2O degradation in human skin, human muscle, chick embryo Echis pyramidum leakeyi ? - ? 94227 3.2.1.35 hyaluronic acid + H2O degradation in human skin, human muscle, chick embryo Echis carinatus sochureki ? - ? 94227 3.2.1.35 hyaluronic acid + H2O degradation in human skin, human muscle, chick embryo Protobothrops elegans ? - ? 94227 3.2.1.35 hyaluronic acid + H2O spreading factor for dermonecrosis Chrysaora quinquecirrha ? - ? 94227 3.2.1.35 hyaluronic acid + H2O spreading factor for dermonecrosis Synanceia horrida ? - ? 94227 3.2.1.35 hyaluronic acid + H2O spreading factor for dermonecrosis Chersonesometrus fulvipes ? - ? 94227 3.2.1.35 hyaluronic acid + H2O involved in inflammatory reactions, exists as inactive form activated by metal ions Homo sapiens ? - ? 94227 3.2.1.35 hyaluronic acid + H2O important in hyaluronate catabolism in the vitreous Homo sapiens ? - ? 94227 3.2.1.35 hyaluronic acid + H2O endo-mucopolysaccharidase hydrolysing acid mucopolysaccharides from connective tissue and chondroitin-sulfate in cartilage Nephrops norvegicus ? - ? 94227 3.2.1.35 hyaluronic acid + H2O two isoforms necessary for penetration of extracellular vestments surrounding eggs prior fertilization Mus musculus ? - ? 94227 3.2.1.35 hyaluronic acid + H2O from rooster comb Bos taurus ? - ? 94227 3.2.1.35 hyaluronic acid + H2O from rooster comb Apis mellifera ? - ? 94227 3.2.1.35 hyaluronic acid + H2O the Asn-X-Ser/Thr sequence is essential for this activity Bos taurus ? - ? 94227 3.2.1.35 hyaluronic acid + H2O - Caenorhabditis elegans N2 ? - ? 94227 3.2.1.35 hyaluronic acid + H2O - Mus musculus hyaluronic acid oligomers - ? 115725 3.2.1.35 hyaluronic acid + H2O - Homo sapiens hyaluronic acid oligomers - ? 115725 3.2.1.35 hyaluronic acid + H2O - Bos taurus hyaluronic acid oligomers - ? 115725 3.2.1.35 hyaluronic acid + H2O - Nephrops norvegicus hyaluronic acid oligomers - ? 115725 3.2.1.35 hyaluronic acid + H2O - Chrysaora quinquecirrha hyaluronic acid oligomers - ? 115725 3.2.1.35 hyaluronic acid + H2O - Synanceia horrida hyaluronic acid oligomers - ? 115725 3.2.1.35 hyaluronic acid + H2O - Streptococcus dysgalactiae hyaluronic acid oligomers - ? 115725 3.2.1.35 hyaluronic acid + H2O - Chersonesometrus fulvipes hyaluronic acid oligomers - ? 115725 3.2.1.35 hyaluronic acid + H2O complete hydrolysis Bos taurus hyaluronic acid tetrasaccharides + ? - ? 115726 3.2.1.35 hyaluronic acid + H2O - Bos taurus N-acetyl-beta-D-glucosamine + D-glucuronate + ? - ? 364712 3.2.1.35 hyaluronic acid from Streptococcus pyogenes (35 kDa) + H2O - Caenorhabditis elegans ? - ? 452774 3.2.1.35 hyaluronic acid from Streptococcus pyogenes (35 kDa) + H2O - Caenorhabditis elegans N2 ? - ? 452774 3.2.1.35 hyaluronic acid hexasaccharide + H2O not only lower oligosaccharides, but also higher oligosaccharides are produced, transglycosylation activity, multiple disaccharide units are rapidly transferred from one substrate molecule to another Bos taurus ? - r 115728 3.2.1.35 hyaluronic acid hexasaccharide + H2O hydrolysis in disaccharide units, transglycosylation activity, smallest substrate Bos taurus hyaluronic acid octasaccharide + hyaluronic acid disaccharide + hyaluronic acid tetrasaccharide - r 115733 3.2.1.35 hyaluronic acid octasaccharosyl-pyridylaminoside + H2O smallest substrate, hydrolysis of even-numbered oligosaccharides with glucuronic acid at the non-reducing terminal by successive removal of disaccharide units from the non-reducing terminal Bos taurus hexasaccharosyl-pyridylaminoside + hyaluronic acid disaccharide - ? 115734 3.2.1.35 hyaluronic acid-rich cumulus matrix + H2O - Macaca sp. ? - ? 364709 3.2.1.35 hyaluronic acid-rich cumulus matrix + H2O investment of oocyte during sperm invasion Macaca sp. ? - ? 364709 3.2.1.35 additional information hyaluronan is degraded by L-ascorbic acid, D-isoascorbic acid, and dehydroascorbic acid themselves, only slightly by L-gulonic-gamma-lactone, D-saccharic-1,4-lactone, and alpha-D-glucoheptonic-gamma-lactone Bos taurus ? - ? 89 3.2.1.35 additional information no activity with chondroitin 4-sulfate, chondroitin 6-sulfate, dermatan sulfate, chondroitin sulfates D and E, and heparin, no activity with 4-nitrophenyl-beta-glucuronide or 4-nitrophenyl-N-acetyl-beta-glucoaminide Agkistrodon contortrix contortrix ? - ? 89 3.2.1.35 additional information simulation of progress of product distribution from short oligosaccharides with the Monte Carlo method using the probalistic model Bos taurus ? - ? 89 3.2.1.35 additional information the splice variant is enzymatically inactive Homo sapiens ? - ? 89 3.2.1.35 additional information alternative splicing controls the cellular expression of enzymatically active hyaluronidase Homo sapiens ? - ? 89 3.2.1.35 additional information CD44-mediated specific role of certain populations of testicular enzyme in the induction of angiogenesis Mus musculus ? - ? 89 3.2.1.35 additional information enzyme activity in serum from tumour-bearing mice, e.g. H460M or SA87 cell grafted mice, is increased, the level is corrsponding to the tumour mass, not in serum from CB193 cell grafted mice Mus musculus ? - ? 89 3.2.1.35 additional information increased concentration of HYAL1-type enzyme correlates with tumour progression and is a marker for grade (G) 2 or 3 bladder cancer Homo sapiens ? - ? 89 3.2.1.35 additional information the enzyme is a major allergen of bee venom which can induce serious, occasionally fatal, systemic IgE-mediated anaphylactic reactions in humans Apis mellifera ? - ? 89 3.2.1.35 additional information HPH-20 or SPAM1, sperm adhesion molecule 1, is a bifunctional enzyme, which is also an adhesion molecule with binding properties to the cumulus mass surrounding the ovum Homo sapiens ? - ? 89 3.2.1.35 additional information isozyme NNH2 is nontoxic, but indirectly potentiates the hemorrhagic activity of hemorrhagic complex-I Naja naja ? - ? 89 3.2.1.35 additional information the enzyme indirectly potentiates the myotoxicity of VRV-PL-VIII, a phospholipolytic myotoxin, and also the hemorrhagic potency of a hemorrhagic complex-I Naja naja ? - ? 89 3.2.1.35 additional information no activity with chondroitin sulfates, heparin, and different chitosans Naja naja ? - ? 89 3.2.1.35 additional information no activity with chondroitin sulfates, heparin, chitosan, 4-nitrophenyl-N-acetyl-beta-D-glucosaminide, or 4-nitrophenyl-beta-D-glucuronide Naja naja ? - ? 89 3.2.1.35 additional information the jaagsiekte sheep retrovirus receptor Hyal2 interacts specifically with the virus particles of the amphotropic murine leukemia virus-pseudotype vectors/JSRV-pseudotype vector envelope protein, binding kinetics, the binding inhibits virus entry into cells, overview jaagsiekte sheep retrovirus ? - ? 89 3.2.1.35 additional information chondroitin, chondroitin sulfate A, chondroitin sulfate B, chondroitin sulfate C, chondroitin sulfate D, heparin, and chitosan are no substrates Hippasa partita ? - ? 89 3.2.1.35 additional information does not hydrolyze chondroitin sulfate B, heparin, or heparan sulfate Loxosceles intermedia ? - ? 89 3.2.1.35 additional information Hyal 1 is expressed in airway epithelium and may operate in a coordinated fashion to depolymerize hyaluronan during inflammation associated with up-regulation of TNF-alpha and IL-1beta, such as allergen-induced asmatic responses Homo sapiens ? - ? 89 3.2.1.35 additional information Hyal 2 is expressed in airway epithelium and may operate in a coordinated fashion to depolymerize hyaluronan during inflammation associated with up-regulation of TNF-alpha and IL-1beta, such as allergen-induced asmatic responses Homo sapiens ? - ? 89 3.2.1.35 additional information Hyal 3 is expressed in airway epithelium and may operate in a coordinated fashion to depolymerize hyaluronan during inflammation associated with up-regulation of TNF-alpha and IL-1beta, such as allergen-induced asmatic responses Homo sapiens ? - ? 89 3.2.1.35 additional information this gene may play an important role in the development of lung and renal malignancies, the expression of hyaluronidase-1 or hyaluronidase-2 suppresses tumour growth in vivo but not in vitro, HYAL2 inhibits experimental lung metastases in nu/nu mice Homo sapiens ? - ? 89 3.2.1.35 additional information this gene may play an important role in the development of lung and renal malignancies, the expression of hyaluronidase-1 suppresses tumour growth in vivo but not in vitro Homo sapiens ? - ? 89 3.2.1.35 additional information among hyaluronidases Hyal-1 to Hyal-4, only rHuHyal-4 exhibits chondroitinase activity with both 6-S-chondroitin sulfate and 4-S-chondroitin sulfate as standard substrates Homo sapiens ? - ? 89 3.2.1.35 additional information enzyme additionally catalyzes transglycosylation reactions of chondroitin sulfates. Barium salt of chondroitin sulfates enhances transglycosylation, the zinc salt of chondroitin sulfates inhibits transglycosylation Bos taurus ? - ? 89 3.2.1.35 additional information minimum substrate for bovine testicular hyaluronidase is the hyaluronan hexasaccharide Bos taurus ? - ? 89 3.2.1.35 additional information no substrate: chondroitin Talaromyces purpureogenus ? - ? 89 3.2.1.35 additional information no activity with dermatan sulfate and heparan sulfate Loxosceles intermedia ? - ? 89 3.2.1.35 additional information the enzyme depolymerizes various chondroitin sulfate isoforms to a similar extent, suggesting broad substrate specificity Mus musculus ? - ? 89 3.2.1.35 additional information the enzyme strongly prefers D-glucuronic acid-(2-O-sulfate)-GalNAc(6-O-sulfate)-containing sequences typical in chondroitin sulfate D Homo sapiens ? - ? 89 3.2.1.35 sodium hyaluronate + H2O 100% activity Bos grunniens ? - ? 430715