3.2.1.126 (R)-amygdalin - Arabidopsis thaliana ? - ? 390445 3.2.1.126 2-nitrophenyl beta-D-galactoside + H2O - Cicer arietinum 2-nitrophenol + D-galactose - ? 13710 3.2.1.126 2-nitrophenyl beta-D-galactoside + H2O reaction at 16% (isozyme I) or 13% (isozyme II) the rate of 2-nitrophenyl beta-D-glucoside hydrolysis Picea abies 2-nitrophenol + D-galactose - ? 13710 3.2.1.126 2-nitrophenyl beta-D-glucoside + H2O - Cicer arietinum 2-nitrophenol + D-glucose - ? 13707 3.2.1.126 2-nitrophenyl beta-D-glucoside + H2O best substrate Picea abies 2-nitrophenol + D-glucose - ? 13707 3.2.1.126 2-nitrophenyl beta-D-glucoside + H2O 57% of the activity with coniferin Pinus contorta 2-nitrophenol + beta-D-glucose - ? 364699 3.2.1.126 2-nitrophenyl beta-D-xyloside + H2O poor substrate Picea abies 2-nitrophenol + D-xylose - ? 13713 3.2.1.126 4-coumaryl beta-D-glucoside - Oryza sativa Japonica Group coumaric acid + D-glucose - ? 446714 3.2.1.126 4-coumaryl beta-D-glucoside + H2O reaction at 25% (isozyme II) or 4% (isozyme I) the rate of 2-nitrophenyl beta-D-glucoside hydrolysis Picea abies coumaric acid + D-glucose - ? 13711 3.2.1.126 4-methylumbelliferyl beta-D-glucosaminide + H2O poor substrate Cicer arietinum 4-methylumbelliferone + D-glucosamine - ? 13705 3.2.1.126 4-methylumbelliferyl beta-D-glucoside + H2O - Cicer arietinum 4-methylumbelliferone + D-glucose - ? 13704 3.2.1.126 4-methylumbelliferyl beta-D-glucoside + H2O reaction at 79% (isozyme II) or 51% (isozyme I) the rate of 2-nitrophenyl beta-D-glucoside hydrolysis Picea abies 4-methylumbelliferone + D-glucose - ? 13704 3.2.1.126 4-methylumbelliferyl beta-D-glucoside + H2O 16% of the activity with coniferin Pinus contorta 4-methylumbelliferone + beta-D-glucose - ? 364701 3.2.1.126 4-methylumbelliferyl-beta-D-glucoside 9% activity with BGLU46 Arabidopsis thaliana 4-methylumbelliferol + beta-D-glucose - ? 390870 3.2.1.126 4-nitrophenyl beta-D-galactoside + H2O - Cicer arietinum 4-nitrophenol + D-galactose - ? 13709 3.2.1.126 4-nitrophenyl beta-D-galactoside + H2O poor substrate, not Picea abies 4-nitrophenol + D-galactose - ? 13709 3.2.1.126 4-nitrophenyl beta-D-glucoside + H2O - Cicer arietinum 4-nitrophenol + D-glucose - ? 13708 3.2.1.126 4-nitrophenyl beta-D-glucoside + H2O reaction at 56% (isozyme II) or 8% (isozyme I) the rate of 2-nitrophenyl beta-D-glucoside hydrolysis Picea abies 4-nitrophenol + D-glucose - ? 13708 3.2.1.126 4-nitrophenyl beta-D-glucoside + H2O 26% of the activity with coniferin Pinus contorta 4-nitrophenol + beta-D-glucose - ? 364700 3.2.1.126 5-[4-(beta-D-glucopyranosyloxy)-3-methoxyphenylmethylene]-2-thioxothiazolidin-4-one-3-ethanoic acid + H2O synthetic coniferin analog, 45% of the activity with coniferin Pinus contorta ? - ? 364698 3.2.1.126 arbutin 6% activity with BGLU46 Arabidopsis thaliana benzene-1,4-diol + beta-D-glucose - ? 391311 3.2.1.126 arbutin + H2O - Agrobacterium tumefaciens ? - ? 94661 3.2.1.126 coniferin 87% activity with BGLU45 and 8% activity with BGLU46 Arabidopsis thaliana beta-D-glucose + coniferyl aldehyde - ? 391682 3.2.1.126 coniferin + H2O - Triticum aestivum coniferol + D-glucose - ? 13700 3.2.1.126 coniferin + H2O - Petroselinum crispum coniferol + D-glucose - ? 13700 3.2.1.126 coniferin + H2O - Cicer arietinum coniferol + D-glucose - ? 13700 3.2.1.126 coniferin + H2O reaction at 52% (isozyme I) or 38% (isozyme II) the rate of 2-nitrophenyl beta-D-glucoside hydrolysis Picea abies coniferol + D-glucose - ? 13700 3.2.1.126 coniferin + H2O i.e. coniferyl alcohol beta-D-glucoside, (E)-coniferin Pinus banksiana coniferol + D-glucose i.e. trans-coniferyl alcohol ? 13700 3.2.1.126 coniferin + H2O - Oryza sativa Japonica Group D-glucose + coniferol - ? 364697 3.2.1.126 coniferin + H2O natural substrate Agrobacterium tumefaciens D-glucose + coniferol - ? 364697 3.2.1.126 coniferin + H2O 20000fold preference for the natural substrate coniferin over cellobiose Agrobacterium tumefaciens D-glucose + coniferol - ? 364697 3.2.1.126 coniferin + H2O the native substrate is efficiently hydrolyzed, preferred substrates Pinus contorta D-glucose + coniferol - ? 364697 3.2.1.126 coniferin + H2O native substrate, may play a crucial role in stem lignification Pinus contorta D-glucose + coniferol - ? 364697 3.2.1.126 coniferin + H2O plant cell wall enzyme, involved in lignin biosynthesis Picea abies ? - ? 369526 3.2.1.126 ferulic acid p-beta-glucoside + H2O - Cicer arietinum ferulic acid + D-glucose - ? 13706 3.2.1.126 feruloyl glucoside + H2O poor substrate Picea abies ferulic acid + glucose - ? 13712 3.2.1.126 formononetin 7-beta-glucoside + H2O - Cicer arietinum ? - ? 452703 3.2.1.126 additional information very poor substrates are arbutin or indican, no substrates are alpha-glucosides, 4-methylumbelliferyl beta-cellobioside, 4-methylumbelliferyl beta-glucuronide, quercetin 3-rhamnosyl glucoside, quercetin 3-rhamnoside or cellobiose Cicer arietinum ? - ? 89 3.2.1.126 additional information enzyme has a high transglycosylation activity in the presence of alcohols, transglycosylation mechanism Agrobacterium tumefaciens ? - ? 89 3.2.1.126 additional information not: disaccharide-containing glycoside, 4-nitrophenyl beta-cellobioside, 4-methylumbelliferyl alpha-glucoside Pinus contorta ? - ? 89 3.2.1.126 additional information no activity with sinigrin, methyl-beta-D-glucoside, D(+)cellobiose, beta-gentiobiose, D(+)maltose, sucrose, p-nitrophenyl-1-thio-beta-D-glucopyranoside, p-nitrophenyl-alpha-D-glucopyranoside, p-nitrophenyl-N-acetyl-beta-D-glucosaminide, p-nitrophenyl-beta-D-mannopyranoside, p-nitrophenyl-beta-D-xylopyranoside, p-nitrophenyl-beta-L-fucopyranoside and p-nitrophenyl-beta-L-arabinopyranoside Arabidopsis thaliana ? - ? 89 3.2.1.126 o-nitrophenyl beta-D-glucopyranoside + H2O - Agrobacterium tumefaciens beta-D-glucose + o-nitrophenol - ? 364703 3.2.1.126 o-nitrophenyl-beta-D-glucopyranoside most rapidly hydrolysed by BGLU45 and BGLU46 with relative rates of 619% and 449%, respectively, of the rate of p-nitrophenyl-beta-D-glucoside degradation Arabidopsis thaliana o-nitrophenol + beta-D-glucose - ? 393027 3.2.1.126 p-coumaryl beta-D-glucoside 6.9% activity with BGLU45 and 71% activity with BGLU46 Arabidopsis thaliana p-coumaric acid + beta-D-glucose - ? 393063 3.2.1.126 p-nitrophenyl alpha-L-arabinofuranoside + H2O with moderate efficiency Agrobacterium tumefaciens p-nitrophenol + alpha-L-arabinofuranose - ? 94240 3.2.1.126 p-nitrophenyl beta-cellobioside + H2O releases only terminal glucose from the substrate Agrobacterium tumefaciens ? - ? 364707 3.2.1.126 p-nitrophenyl beta-D-fucopyranoside + H2O with moderate efficiency Agrobacterium tumefaciens beta-D-fucose + p-nitrophenol - ? 364705 3.2.1.126 p-nitrophenyl beta-D-galactopyranoside + H2O with low efficiency Agrobacterium tumefaciens beta-D-galactose + p-nitrophenol - ? 364706 3.2.1.126 p-nitrophenyl beta-D-glucopyranoside + H2O - Agrobacterium tumefaciens beta-D-glucose + p-nitrophenol - ? 364702 3.2.1.126 p-nitrophenyl beta-D-xylopyranoside + H2O 73% of the efficiency with p-nitrophenyl beta-D-glucopyranoside Agrobacterium tumefaciens beta-D-xylose + p-nitrophenol - ? 364704 3.2.1.126 p-nitrophenyl-beta-D-galactopyranoside 0.4% activity with BGLU45 and 21% activity with BGLU46 Arabidopsis thaliana p-nitrophenol + beta-D-galactose - ? 393124 3.2.1.126 p-nitrophenyl-beta-D-glucopyranoside 100% activity with BGLU45 and BGLU46 Arabidopsis thaliana p-nitrophenol + beta-D-glucose - ? 393126 3.2.1.126 phenyl-beta-D-glucoside 62% activity with BGLU46 Arabidopsis thaliana phenol + beta-D-glucose - ? 393214 3.2.1.126 picein + H2O - Cicer arietinum ? - ? 13702 3.2.1.126 picein + H2O reaction at 50% (isozyme II) or 12% (isozyme I) the rate of 2-nitrophenyl beta-D-glucoside hydrolysis Picea abies ? - ? 13702 3.2.1.126 salicin 100% activity with BGLU46 Arabidopsis thaliana beta-D-glucose + salicyl alcohol - ? 393461 3.2.1.126 salicin + H2O - Agrobacterium tumefaciens ? - ? 13703 3.2.1.126 salicin + H2O poor substrate Cicer arietinum ? - ? 13703 3.2.1.126 salicin + H2O reaction at 25% (isozyme II) or 2% (isozyme I) the rate of 2-nitrophenyl beta-D-glucoside hydrolysis Picea abies ? - ? 13703 3.2.1.126 syringin + H2O - Oryza sativa Japonica Group D-glucose + sinapyl alcohol - ? 13701 3.2.1.126 syringin + H2O poor substrate Cicer arietinum D-glucose + sinapyl alcohol - ? 13701 3.2.1.126 syringin + H2O reaction at about 30% the rate of 2-nitrophenyl beta-D-glucoside hydrolysis Picea abies D-glucose + sinapyl alcohol - ? 13701 3.2.1.126 syringin + H2O 51% of the activity with coniferin Pinus contorta D-glucose + sinapyl alcohol - ? 13701 3.2.1.126 syringin + H2O 100% activity with BGLU45 and 6% activity with BGLU46 Arabidopsis thaliana D-glucose + sinapyl alcohol - ? 13701