3.2.1.10 1,6-di-O-alpha-D-glucitol + H2O - Sus scrofa D-glucitol - ? 371466 3.2.1.10 1,6-di-O-alpha-D-glucopyranosyl-D-fructofuranose + H2O - Sus scrofa D-glucose + D-fructose - ? 371468 3.2.1.10 1,6-di-O-alpha-D-mannitol + H2O - Sus scrofa D-mannitol - ? 371469 3.2.1.10 1-O-alpha-D-glucopyranosyl-D-fructopyranose + H2O - Sus scrofa D-fructose - ? 371513 3.2.1.10 1-O-alpha-D-glucopyranosyl-D-glucitol + H2O - Sus scrofa D-glucose + D-glucitol - ? 371515 3.2.1.10 1-O-alpha-D-glucopyranosyl-D-mannitol + H2O - Sus scrofa D-glucose + D-mannitol - ? 371517 3.2.1.10 2'-deoxy-methyl-alpha-isomaltoside + H2O - Saccharomyces cerevisiae ? - ? 371564 3.2.1.10 2-deoxy-methyl-alpha-isomaltoside + H2O - Saccharomyces cerevisiae ? - ? 371666 3.2.1.10 3'-deoxy-methyl-alpha-isomaltoside + H2O - Saccharomyces cerevisiae ? - ? 371786 3.2.1.10 3-deoxy-methyl-alpha-isomaltoside + H2O - Saccharomyces cerevisiae ? - ? 371832 3.2.1.10 4'-deoxy-methyl-alpha-isomaltoside + H2O - Saccharomyces cerevisiae ? - ? 371898 3.2.1.10 4-deoxy-methyl-alpha-isomaltoside + H2O - Saccharomyces cerevisiae ? - ? 371934 3.2.1.10 4-methylumbelliferyl alpha-D-glucoside + H2O - Bifidobacterium adolescentis 4-methylumbelliferol + alpha-D-glucose - ? 371993 3.2.1.10 4-methylumbelliferyl beta-D-glucoside + H2O - Rattus norvegicus 4-methylumbelliferol + beta-D-glucose - ? 371999 3.2.1.10 4-nitrophenyl 1-O-alpha-D-glucopyranoside + H2O - Bacillus sp. (in: Bacteria) 4-nitrophenol + alpha-D-glucopyranose - ? 372049 3.2.1.10 4-nitrophenyl 1-O-alpha-D-glucopyranoside + H2O - Bacillus sp. (in: Bacteria) SAM1606 4-nitrophenol + alpha-D-glucopyranose - ? 372049 3.2.1.10 4-nitrophenyl alpha-D-glucopyranoside - Saccharomyces cerevisiae 4-nitrophenol + alpha-D-glucopyranose - ? 372052 3.2.1.10 4-nitrophenyl alpha-D-glucopyranoside + H2O - Saccharomyces cerevisiae 4-nitrophenol + alpha-D-glucopyranose - ? 372051 3.2.1.10 4-nitrophenyl alpha-D-glucopyranoside + H2O - Bacillus cereus 4-nitrophenol + alpha-D-glucopyranose - ? 372051 3.2.1.10 4-nitrophenyl alpha-D-glucopyranoside + H2O - Arthrobacter globiformis 4-nitrophenol + alpha-D-glucopyranose - ? 372051 3.2.1.10 4-nitrophenyl alpha-D-glucopyranoside + H2O - Parageobacillus thermoglucosidasius 4-nitrophenol + alpha-D-glucopyranose - ? 372051 3.2.1.10 4-nitrophenyl alpha-D-glucopyranoside + H2O - Bifidobacterium adolescentis 4-nitrophenol + alpha-D-glucopyranose - ? 372051 3.2.1.10 4-nitrophenyl alpha-D-glucopyranoside + H2O - Homo sapiens 4-nitrophenol + alpha-D-glucopyranose - ? 372051 3.2.1.10 4-nitrophenyl alpha-D-glucopyranoside + H2O - Parageobacillus thermoglucosidasius KP1006 4-nitrophenol + alpha-D-glucopyranose - ? 372051 3.2.1.10 4-nitrophenyl alpha-D-glucopyranoside + H2O - Arthrobacter globiformis I42 4-nitrophenol + alpha-D-glucopyranose - ? 372051 3.2.1.10 4-nitrophenyl-alpha-D-glucopyranoside + H2O - Lactiplantibacillus plantarum subsp. plantarum 4-nitrophenol + D-glucose - ? 431400 3.2.1.10 4-nitrophenyl-alpha-D-glucopyranoside + H2O - Lactiplantibacillus plantarum subsp. plantarum ATCC 149917 4-nitrophenol + D-glucose - ? 431400 3.2.1.10 4-nitrophenyl-alpha-D-glucopyranoside + H2O - Lactiplantibacillus plantarum subsp. plantarum LL441 4-nitrophenol + D-glucose - ? 431400 3.2.1.10 4-O-alpha-D-glucopyranosyl-D-glucopyranose + H2O - Sus scrofa D-glucose - ? 372091 3.2.1.10 6'-deoxy-methyl-alpha-isomaltoside + H2O - Saccharomyces cerevisiae ? - ? 372175 3.2.1.10 6(R)-ethyl-alpha-isomaltoside + H2O - Saccharomyces cerevisiae ? - ? 372179 3.2.1.10 6(S)-ethyl-alpha-isomaltoside + H2O - Saccharomyces cerevisiae ? - ? 372180 3.2.1.10 6-O-alpha-D-glucopyranosyl-D-fructofuranose + H2O - Sus scrofa D-glucose + D-fructose - ? 372190 3.2.1.10 6-O-alpha-D-glucopyranosyl-D-glucitol + H2O - Sus scrofa D-glucose + D-glucitol - ? 372192 3.2.1.10 6-O-alpha-D-glucopyranosyl-D-glucopyranose + H2O - Sus scrofa D-glucose - ? 372193 3.2.1.10 alpha-D-glucopyranosyl-(1-6)-alpha-D-glucopyranosyl-(1-1)-D-glucitol + H2O - Sus scrofa D-glucose + D-glucitol - ? 372312 3.2.1.10 alpha-D-glucopyranosyl-(1-6)-alpha-D-glucopyranosyl-(1-1)-D-mannitol + H2O - Sus scrofa D-glucose + D-mannitol - ? 372313 3.2.1.10 alpha-D-glucopyranosyl-(1-6)-alpha-D-glucopyranosyl-(1-6)-alpha-D-glucopyranosyl-(1-1)-D-mannitol + H2O - Sus scrofa D-glucose + D-mannitol - ? 372314 3.2.1.10 alpha-D-glucopyranosyl-(1-6)-alpha-D-glucopyranosyl-(1-6)-alpha-D-glucopyranosyl-(1-6)-D-glucitol + H2O - Sus scrofa D-glucose + D-glucitol - ? 372315 3.2.1.10 alpha-D-glucopyranosyl-(1-6)-alpha-D-glucopyranosyl-(1-6)-alpha-D-glucopyranosyl-D-fructofuranose + H2O - Sus scrofa D-glucose + D-fructose - ? 372316 3.2.1.10 alpha-D-glucopyranosyl-(1-6)-alpha-D-glucopyranosyl-(1-6)-D-fructofuranose + H2O - Sus scrofa D-glucose + D-fructose - ? 372318 3.2.1.10 alpha-D-glucopyranosyl-(1-6)-alpha-D-glucopyranosyl-(1-6)-D-glucitol + H2O - Sus scrofa D-glucose + D-glucitol - ? 372320 3.2.1.10 alpha-D-glucopyranosyl-(1-6)-alpha-D-glucopyranosyl-(1-6)-D-glucopyranose + H2O - Sus scrofa D-glucose - ? 372322 3.2.1.10 alpha-D-glucopyranosyl-(1-6)-alpha-D-glucopyranosyl-D-fructofuranose + H2O - Sus scrofa D-glucose + D-fructose - ? 372324 3.2.1.10 alpha-D-glucopyranosyl-(1-6)-beta-fructofuranosyl-alpha-D-glucopyranoside + H2O - Sus scrofa D-glucose + D-fructose - ? 372326 3.2.1.10 alpha-limit dextrin + H2O - Homo sapiens ? - ? 399934 3.2.1.10 alpha-limit dextrins + H2O - Sus scrofa D-glucose - ? 214430 3.2.1.10 alpha-limit dextrins + H2O - Bacillus cereus D-glucose - ? 214430 3.2.1.10 alpha-limit dextrins + H2O - Weizmannia coagulans D-glucose - ? 214430 3.2.1.10 amylose + H2O n = 18, low activity of wild-type sucrase-isomaltase, very low activity of mutant isomaltase Suncus murinus ? - ? 94440 3.2.1.10 glycogen + H2O substrate from oyster, low activity of wild-type sucrase-isomaltase, very low activity of mutant isomaltase Suncus murinus ? - ? 94449 3.2.1.10 isomaltohexaose + H2O - Bacillus cereus 6 alpha-D-glucose - ? 452694 3.2.1.10 isomaltohexaose + H2O - Weizmannia coagulans 6 alpha-D-glucose - ? 452694 3.2.1.10 isomaltopentaose + H2O - Bacillus cereus 5 alpha-D-glucose - ? 452692 3.2.1.10 isomaltopentaose + H2O - Weizmannia coagulans 5 alpha-D-glucose - ? 452692 3.2.1.10 isomaltopentaose + H2O - Parageobacillus thermoglucosidasius 5 alpha-D-glucose - ? 452692 3.2.1.10 isomaltosaccharides + H2O - Bacillus cereus D-glucose - ? 214425 3.2.1.10 isomaltosaccharides + H2O - Parageobacillus thermoglucosidasius D-glucose - ? 214425 3.2.1.10 isomaltosaccharides + H2O n = 2-6 Bacillus cereus D-glucose - ? 214425 3.2.1.10 isomaltosaccharides + H2O n = 2-6 Weizmannia coagulans D-glucose - ? 214425 3.2.1.10 isomaltosaccharides + H2O n = 2-6 Bacillus sp. F5 D-glucose - ? 214425 3.2.1.10 isomaltosaccharides + H2O n = 3-5 Parageobacillus thermoglucosidasius D-glucose - ? 214425 3.2.1.10 isomaltosaccharides + H2O n = 2-3 Thermoanaerobium sp. D-glucose - ? 214425 3.2.1.10 isomaltosaccharides + H2O n = 2, isomaltose Bacillus subtilis D-glucose - ? 214425 3.2.1.10 isomaltosaccharides + H2O n = 2, isomaltose Homo sapiens D-glucose - ? 214425 3.2.1.10 isomaltosaccharides + H2O n = 2, isomaltose Rattus norvegicus D-glucose - ? 214425 3.2.1.10 isomaltosaccharides + H2O n = 2, isomaltose Sus scrofa D-glucose - ? 214425 3.2.1.10 isomaltosaccharides + H2O n = 2, isomaltose Zalophus californianus D-glucose - ? 214425 3.2.1.10 isomaltosaccharides + H2O n = 2, isomaltose Callorhinus ursinus D-glucose - ? 214425 3.2.1.10 isomaltosaccharides + H2O - Parageobacillus thermoglucosidasius KP 1006 D-glucose - ? 214425 3.2.1.10 isomaltosaccharides + H2O - Bacillus cereus NY-14 D-glucose - ? 214425 3.2.1.10 isomaltose + H2O - Bacillus subtilis 2 D-glucose - ? 94409 3.2.1.10 isomaltose + H2O - Homo sapiens 2 D-glucose - ? 94409 3.2.1.10 isomaltose + H2O - Rattus norvegicus 2 D-glucose - ? 94409 3.2.1.10 isomaltose + H2O - Sus scrofa 2 D-glucose - ? 94409 3.2.1.10 isomaltose + H2O - Bacillus cereus 2 D-glucose - ? 94409 3.2.1.10 isomaltose + H2O - Thermoanaerobium sp. 2 D-glucose - ? 94409 3.2.1.10 isomaltose + H2O - Parageobacillus thermoglucosidasius 2 D-glucose - ? 94409 3.2.1.10 isomaltose + H2O - Zalophus californianus 2 D-glucose - ? 94409 3.2.1.10 isomaltose + H2O - Callorhinus ursinus 2 D-glucose - ? 94409 3.2.1.10 isomaltose + H2O - Bacillus sp. F5 2 D-glucose - ? 94409 3.2.1.10 isomaltose + H2O - Alkalihalophilus pseudofirmus 2 D-glucose - ? 94409 3.2.1.10 isomaltose + H2O - Saccharomyces cerevisiae 2 D-glucose - ? 94409 3.2.1.10 isomaltose + H2O best substrate for the mutant isomaltase Suncus murinus 2 D-glucose - ? 94409 3.2.1.10 isomaltose + H2O the hydrolysing percentages are 54.06% Lactiplantibacillus plantarum subsp. plantarum 2 D-glucose - ? 94409 3.2.1.10 isomaltose + H2O the hydrolysing percentages are 54.06% Lactiplantibacillus plantarum subsp. plantarum ATCC 149917 2 D-glucose - ? 94409 3.2.1.10 isomaltose + H2O - Thermoanaerobium sp. Tok6-B1 2 D-glucose - ? 94409 3.2.1.10 isomaltose + H2O - Parageobacillus thermoglucosidasius KP 1006 2 D-glucose - ? 94409 3.2.1.10 isomaltose + H2O the hydrolysing percentages are 54.06% Lactiplantibacillus plantarum subsp. plantarum LL441 2 D-glucose - ? 94409 3.2.1.10 isomaltose + H2O - Alkalihalophilus pseudofirmus 703 2 D-glucose - ? 94409 3.2.1.10 isomaltose + H2O - Bacillus cereus NY-14 2 D-glucose - ? 94409 3.2.1.10 isomaltose + H2O - Bacillus sp. (in: Bacteria) ? - ? 139855 3.2.1.10 isomaltose + H2O - Arthrobacter globiformis ? - ? 139855 3.2.1.10 isomaltose + H2O - Bifidobacterium adolescentis ? - ? 139855 3.2.1.10 isomaltose + H2O - Bacillus sp. (in: Bacteria) SAM1606 ? - ? 139855 3.2.1.10 isomaltose + H2O - Arthrobacter globiformis I42 ? - ? 139855 3.2.1.10 isomaltose + H2O - Saccharomyces cerevisiae alpha-D-glucose + D-glucose - ? 401185 3.2.1.10 isomaltose + H2O - Weizmannia coagulans alpha-D-glucose + D-glucose - ? 401185 3.2.1.10 isomaltose + H2O - Bifidobacterium breve alpha-D-glucose + D-glucose - ? 401185 3.2.1.10 isomaltose + H2O i.e. alpha-D-Glc-(1->6)-D-Glc. Multifunctional enzyme that catalyzes also terminal alpha-1,4-linked, alpha-1,2-linked and alpha-1,3-linked glucose residues (EC 3.2.1.20/kojibiose/EC 3.2.1.84 hydrolase) Sulfolobus acidocaldarius alpha-D-glucose + D-glucose - ? 401185 3.2.1.10 isomaltose + H2O i.e. alpha-D-Glc-(1->6)-D-Glc. Multifunctional enzyme that catalyzes also terminal alpha-1,4-linked, alpha-1,2-linked and alpha-1,3-linked glucose residues (EC 3.2.1.20/kojibiose/EC 3.2.1.84 hydrolase) Sulfolobus acidocaldarius DSM 639 alpha-D-glucose + D-glucose - ? 401185 3.2.1.10 isomaltose + H2O - Bifidobacterium breve UCC2003 alpha-D-glucose + D-glucose - ? 401185 3.2.1.10 isomaltotetraose + H2O - Bacillus cereus 4 alpha-D-glucose - ? 452693 3.2.1.10 isomaltotetraose + H2O - Weizmannia coagulans 4 alpha-D-glucose - ? 452693 3.2.1.10 isomaltotetraose + H2O - Parageobacillus thermoglucosidasius 4 alpha-D-glucose - ? 452693 3.2.1.10 isomaltotriose + H2O - Bacillus cereus 3 alpha-D-glucose - ? 401184 3.2.1.10 isomaltotriose + H2O - Weizmannia coagulans 3 alpha-D-glucose - ? 401184 3.2.1.10 isomaltotriose + H2O - Parageobacillus thermoglucosidasius 3 alpha-D-glucose - ? 401184 3.2.1.10 isomaltotriose + H2O - Bacillus sp. F5 3 alpha-D-glucose - ? 401184 3.2.1.10 isomaltotriose + H2O - Bifidobacterium breve 3 alpha-D-glucose - ? 401184 3.2.1.10 isomaltotriose + H2O - Bifidobacterium breve UCC2003 3 alpha-D-glucose - ? 401184 3.2.1.10 isomaltriose + H2O - Bifidobacterium adolescentis ? - ? 373491 3.2.1.10 isomaltriose + H2O - Saccharomyces cerevisiae ? - ? 373491 3.2.1.10 isomaltulose + H2O the hydrolysing percentages are 37.7% Lactiplantibacillus plantarum subsp. plantarum D-glucose + D-fructose - ? 447631 3.2.1.10 isomaltulose + H2O the hydrolysing percentages are 37.7% Lactiplantibacillus plantarum subsp. plantarum ATCC 149917 D-glucose + D-fructose - ? 447631 3.2.1.10 isomaltulose + H2O the hydrolysing percentages are 37.7% Lactiplantibacillus plantarum subsp. plantarum LL441 D-glucose + D-fructose - ? 447631 3.2.1.10 kojibiose + H2O weak activity Parageobacillus thermoglucosidasius 2 D-glucose - ? 214432 3.2.1.10 maltodextrin + H2O corn maltodextrin Homo sapiens ? - ? 259754 3.2.1.10 maltoheptaose + H2O low activity of mutant isomaltase Suncus murinus ? - ? 94186 3.2.1.10 maltohexaose + H2O low activity of mutant isomaltase Suncus murinus ? - ? 94185 3.2.1.10 maltopentaose + H2O - Suncus murinus ? - ? 94184 3.2.1.10 maltopentose + H2O - Homo sapiens ? - ? 373760 3.2.1.10 maltose + H2O - Bacillus subtilis 2 D-glucose - ? 94406 3.2.1.10 maltose + H2O - Homo sapiens 2 D-glucose - ? 94406 3.2.1.10 maltose + H2O - Rattus norvegicus 2 D-glucose - ? 94406 3.2.1.10 maltose + H2O - Sus scrofa 2 D-glucose - ? 94406 3.2.1.10 maltose + H2O - Zalophus californianus 2 D-glucose - ? 94406 3.2.1.10 maltose + H2O - Bacillus sp. F5 2 D-glucose - ? 94406 3.2.1.10 maltose + H2O - Alkalihalophilus pseudofirmus 2 D-glucose - ? 94406 3.2.1.10 maltose + H2O best substrate of the wild-type sucrase-isomaltase Suncus murinus 2 D-glucose - ? 94406 3.2.1.10 maltose + H2O - Alkalihalophilus pseudofirmus 703 2 D-glucose - ? 94406 3.2.1.10 maltose + H2O - Homo sapiens D-glucose + D-glucose - ? 401349 3.2.1.10 maltotetraose + H2O - Homo sapiens ? - ? 94183 3.2.1.10 maltotetraose + H2O low activity of mutant isomaltase Suncus murinus ? - ? 94183 3.2.1.10 maltotriitol + H2O - Bacillus sp. F5 D-glucose + maltitol - ? 214434 3.2.1.10 maltotriose + H2O - Homo sapiens ? - ? 94410 3.2.1.10 maltotriose + H2O low activity of mutant isomaltase Suncus murinus ? - ? 94410 3.2.1.10 maltotriose + H2O - Bacillus sp. F5 maltose + D-glucose - ? 214435 3.2.1.10 maltotriose + H2O - Alkalihalophilus pseudofirmus maltose + D-glucose - ? 214435 3.2.1.10 maltotriose + H2O - Alkalihalophilus pseudofirmus 703 maltose + D-glucose - ? 214435 3.2.1.10 maltulose + H2O - Bifidobacterium breve alpha-D-glucose + D-fructose - ? 401351 3.2.1.10 maltulose + H2O - Bifidobacterium breve UCC2003 alpha-D-glucose + D-fructose - ? 401351 3.2.1.10 methyl alpha-D-glucopyranoside + H2O - Saccharomyces cerevisiae methanol + alpha-D-glucopyranose - ? 373819 3.2.1.10 methyl-alpha-isomaltoside + H2O - Saccharomyces cerevisiae ? - ? 373829 3.2.1.10 additional information no degradation of p-nitrophenyl-beta-D-glucose, p-nitrophenyl-alpha-D-galactose, p-nitrophenyl-beta-D-galactose, p-nitrophenyl-beta-D-xylose, p-nitrophenyl-alpha-L-arabinose, N-acetyl-alpha-D-glucosamine at 1 mM under standard conditions Thermoanaerobium sp. ? - ? 89 3.2.1.10 additional information no maltase activity Parageobacillus thermoglucosidasius ? - ? 89 3.2.1.10 additional information evolutionary connection to other alpha-glucosidases in comparison of amino acid sequence and substrate specificity, overview, the alpha-glucosidase, EC 3.2.1.20, of Bacillus sp. strain SAM1606 shows 192fold enhanced activity with trehalose by mutations P273G and T342N Bacillus sp. (in: Bacteria) ? - ? 89 3.2.1.10 additional information phenotype II of congenital enzyme deficiency features the retention of the brush border protein in the cis-Golgi, this transport is blocked by substitution of Gln1098 by proline Homo sapiens ? - ? 89 3.2.1.10 additional information tertiary structure overview and evolutionary tree of subfamilies of the alpha-amylase family, including the oligo-1,6-glucosidase subfamily, analysis by comparison of the fifth conserved amino acid sequence region, overview Bacillus cereus ? - ? 89 3.2.1.10 additional information tertiary structure overview and evolutionary tree of subfamilies of the alpha-amylase family, including the oligo-1,6-glucosidase subfamily, analysis by comparison of the fifth conserved amino acid sequence region, overview Weizmannia coagulans ? - ? 89 3.2.1.10 additional information tertiary structure overview and evolutionary tree of subfamilies of the alpha-amylase family, including the oligo-1,6-glucosidase subfamily, analysis by comparison of the fifth conserved amino acid sequence region, overview Bacillus sp. (in: Bacteria) ? - ? 89 3.2.1.10 additional information tertiary structure overview and evolutionary tree of subfamilies of the alpha-amylase family, including the oligo-1,6-glucosidase subfamily, analysis by comparison of the fifth conserved amino acid sequence region, overview Erwinia rhapontici ? - ? 89 3.2.1.10 additional information tertiary structure overview and evolutionary tree of subfamilies of the alpha-amylase family, including the oligo-1,6-glucosidase subfamily, analysis by comparison of the fifth conserved amino acid sequence region, overview Parageobacillus thermoglucosidasius ? - ? 89 3.2.1.10 additional information tertiary structure overview and evolutionary tree of subfamilies of the alpha-amylase family, including the oligo-1,6-glucosidase subfamily, analysis by comparison of the fifth conserved amino acid sequence region, overview Bacillus flavocaldarius ? - ? 89 3.2.1.10 additional information tertiary structure overview and evolutionary tree of subfamilies of the alpha-amylase family, including the oligo-1,6-glucosidase subfamily, analysis by comparison of the fifth conserved amino acid sequence region, overview Bacillus subtilis ? - ? 89 3.2.1.10 additional information the enzyme might be involved in degradation for utilization of dextran in the bacterium Arthrobacter globiformis ? - ? 89 3.2.1.10 additional information enzyme can also function as a cAMP-dependent chloride channel Necturus sp. ? - ? 89 3.2.1.10 additional information enzyme is highly specific for alpha-1,6-linkages Bacillus sp. (in: Bacteria) ? - ? 89 3.2.1.10 additional information N-terminal domain alpha/beta motifs are probably recognized by the endogenous GroEL protein, leading to binding and stabilization Parageobacillus thermoglucosidasius ? - ? 89 3.2.1.10 additional information stereochemistry and -specificity, overview Saccharomyces cerevisiae ? - ? 89 3.2.1.10 additional information substrate specificities of mutant isomaltase and wild-type sucrase-isomaltase, overview Suncus murinus ? - ? 89 3.2.1.10 additional information substrate specificities of the 2 enzyme activities with the different substrate variants, overview, the active site of the sucrase subunit cleaves alpha,beta(1-2)-glycosidic bonds, and only 2 monomer units of the substrates bind with favourable affinity, while the isomaltase subunit possesses more than 2 subsites and cleaves oligosaccharides and reduced oligosaccharides with alpha(1-6)- and alpha(1-1)-glycosidic bonds, the isomaltase has binding sites for various aglycons Sus scrofa ? - ? 89 3.2.1.10 additional information substrate specificity, enzyme is also capable to perform a transglycosylation producing oligosaccharides from trehalose and with low activity from sucrose, but no hydrolytic activity is detected with sucrose Bifidobacterium adolescentis ? - ? 89 3.2.1.10 additional information N-terminal sucrase-isomaltase has a broad specificity for both alpha-1,4- and alpha-1,6-oligosaccharides Homo sapiens ? - ? 89 3.2.1.10 additional information substrate specificities of isozymes IMA1 to IMA5, overview Saccharomyces cerevisiae ? - ? 89 3.2.1.10 additional information the best substrate for oligo-1,6-glucosidase is isomaltotriose, other, longer-chain oligosaccharides are also good substrates. Isomaltase shows the highest activity towards isomaltose and very little activity towards longer oligosaccharides, because the entrance to the active site pocket of isomaltose is severely narrowed by Tyr158, His280, and loop 310–315, and because the isomaltase pocket is shallower than that of other oligo-1,6-glucosidases, isomaltase substrate specificity, overview Saccharomyces cerevisiae ? - ? 89 3.2.1.10 additional information the enzyme is active with maltose, maltotriose, and sucrose, only the N-terminal catalytic domain acts as alpha-1,6-glucosidase Homo sapiens ? - ? 89 3.2.1.10 additional information isoforms Ima1, Ima2, Ima3 and Ima5 exhibit a preference for the alpha-(1,6) disaccharides isomaltose and palatinose, with Michaelis-Menten kinetics and inhibition at high substrates concentration. They are also able to hydrolyze trisaccharides bearing an alpha-(1,6) linkage, but also alpha-(1,2), alpha-(1,3) and alpha-(1,5) disaccharides including sucrose, highlighting their substrate ambiguity. No substrate: melibiose Saccharomyces cerevisiae ? - ? 89 3.2.1.10 additional information isoforms Ima1, Ima2, Ima3 and Ima5 exhibit a preference for the alpha-(1,6) disaccharides isomaltose and palatinose, with Michaelis–Menten kinetics and inhibition at high substrates concentration. They are also able to hydrolyze trisaccharides bearing an alpha-(1,6) linkage, but also alpha-(1,2), alpha-(1,3) and alpha-(1,5) disaccharides including sucrose, highlighting their substrate ambiguity. No substrate: melibiose Saccharomyces cerevisiae ? - ? 89 3.2.1.10 additional information the alpha-glucosidase (EC 3.2.1.20, UniProt ID A0A1S6JYL1) from Bacillus pseudofirmus strain 703 also shows oligo-alpha-1,6-glucosidase activity (EC 3.2.1.10), having both exo-alpha-1,4-glucosidase and oligo-1,6-glucosidase activities, but it shows no transglycosylation activity. The enzyme exhibits high activity against amylopectin from maize and soluble starch, but no activity against amylose from potato, pullulan, carboxymethylcellulose, and beachwood xylan. Amy112 hydrolyzes alpha-1,6-glucosidic linkages less effective than alpha-1,4-glucosidic linkages Alkalihalophilus pseudofirmus ? - ? 89 3.2.1.10 additional information the enzyme degrades isomaltose and isomaltulose, but not isomaltrotriose, melibiose, panose and raffinose. The enzyme shows no activity with trehalose, sucrose, turanose or maltose, i.e. sugars with glycosidic bonds other than 1->6, nor with 4-nitrophenyl-beta-D-glucopyranoside. Isomaltose and isomaltulose are the preferred substrates. Under the conditions of the assay, no transglycosylation activity of recombinant His-tagged enzyme MalL is observed with respect to the disaccharides sucrose, maltose, gentibiose, panose and isomaltulose Lactiplantibacillus plantarum subsp. plantarum ? - ? 89 3.2.1.10 additional information the enzyme performs hydrolysis of sucrose and maltose by an alpha-D-glucosidase-type action (EC 3.2.1.48), and hydrolysis of (1->6)-alpha-D-glucosidic linkages in some oligosaccharides produced from starch and glycogen by alpha-amylase, and in isomaltose (EC 3.2.1.10), reaction mechanism Homo sapiens ? - ? 89 3.2.1.10 additional information the enzyme degrades isomaltose and isomaltulose, but not isomaltrotriose, melibiose, panose and raffinose. The enzyme shows no activity with trehalose, sucrose, turanose or maltose, i.e. sugars with glycosidic bonds other than 1->6, nor with 4-nitrophenyl-beta-D-glucopyranoside. Isomaltose and isomaltulose are the preferred substrates. Under the conditions of the assay, no transglycosylation activity of recombinant His-tagged enzyme MalL is observed with respect to the disaccharides sucrose, maltose, gentibiose, panose and isomaltulose Lactiplantibacillus plantarum subsp. plantarum ATCC 149917 ? - ? 89 3.2.1.10 additional information N-terminal domain alpha/beta motifs are probably recognized by the endogenous GroEL protein, leading to binding and stabilization Parageobacillus thermoglucosidasius KP1006 ? - ? 89 3.2.1.10 additional information tertiary structure overview and evolutionary tree of subfamilies of the alpha-amylase family, including the oligo-1,6-glucosidase subfamily, analysis by comparison of the fifth conserved amino acid sequence region, overview Bacillus sp. (in: Bacteria) F5 ? - ? 89 3.2.1.10 additional information evolutionary connection to other alpha-glucosidases in comparison of amino acid sequence and substrate specificity, overview, the alpha-glucosidase, EC 3.2.1.20, of Bacillus sp. strain SAM1606 shows 192fold enhanced activity with trehalose by mutations P273G and T342N Bacillus sp. (in: Bacteria) SAM1606 ? - ? 89 3.2.1.10 additional information enzyme is highly specific for alpha-1,6-linkages Bacillus sp. (in: Bacteria) SAM1606 ? - ? 89 3.2.1.10 additional information no degradation of p-nitrophenyl-beta-D-glucose, p-nitrophenyl-alpha-D-galactose, p-nitrophenyl-beta-D-galactose, p-nitrophenyl-beta-D-xylose, p-nitrophenyl-alpha-L-arabinose, N-acetyl-alpha-D-glucosamine at 1 mM under standard conditions Thermoanaerobium sp. Tok6-B1 ? - ? 89 3.2.1.10 additional information the enzyme might be involved in degradation for utilization of dextran in the bacterium Arthrobacter globiformis I42 ? - ? 89 3.2.1.10 additional information no maltase activity Parageobacillus thermoglucosidasius KP 1006 ? - ? 89 3.2.1.10 additional information the enzyme degrades isomaltose and isomaltulose, but not isomaltrotriose, melibiose, panose and raffinose. The enzyme shows no activity with trehalose, sucrose, turanose or maltose, i.e. sugars with glycosidic bonds other than 1->6, nor with 4-nitrophenyl-beta-D-glucopyranoside. Isomaltose and isomaltulose are the preferred substrates. Under the conditions of the assay, no transglycosylation activity of recombinant His-tagged enzyme MalL is observed with respect to the disaccharides sucrose, maltose, gentibiose, panose and isomaltulose Lactiplantibacillus plantarum subsp. plantarum LL441 ? - ? 89 3.2.1.10 additional information the alpha-glucosidase (EC 3.2.1.20, UniProt ID A0A1S6JYL1) from Bacillus pseudofirmus strain 703 also shows oligo-alpha-1,6-glucosidase activity (EC 3.2.1.10), having both exo-alpha-1,4-glucosidase and oligo-1,6-glucosidase activities, but it shows no transglycosylation activity. The enzyme exhibits high activity against amylopectin from maize and soluble starch, but no activity against amylose from potato, pullulan, carboxymethylcellulose, and beachwood xylan. Amy112 hydrolyzes alpha-1,6-glucosidic linkages less effective than alpha-1,4-glucosidic linkages Alkalihalophilus pseudofirmus 703 ? - ? 89 3.2.1.10 nigerose + H2O weak activity Thermoanaerobium sp. 2 D-glucose - ? 214422 3.2.1.10 nigerose + H2O weak activity Parageobacillus thermoglucosidasius 2 D-glucose - ? 214422 3.2.1.10 nigerose + H2O weak activity Thermoanaerobium sp. Tok6-B1 2 D-glucose - ? 214422 3.2.1.10 nigerose + H2O weak activity Parageobacillus thermoglucosidasius KP 1006 2 D-glucose - ? 214422 3.2.1.10 nigerose + H2O - Bifidobacterium breve alpha-D-glucose + D-glucose - ? 401533 3.2.1.10 nigerose + H2O - Bifidobacterium breve UCC2003 alpha-D-glucose + D-glucose - ? 401533 3.2.1.10 oligosaccharide + H2O role in digestion of carbohydrates Sus scrofa monosaccharide - ? 370048 3.2.1.10 oligosaccharide + H2O role in starch degradation Pisum sativum monosaccharide - ? 370048 3.2.1.10 oligosaccharide + H2O role in absorption of sucrose, isomaltose, maltose Homo sapiens monosaccharide - ? 370048 3.2.1.10 oligosaccharide + H2O direct function in maltose and isomaltose metabolism Bacillus subtilis monosaccharide - ? 370048 3.2.1.10 p-nitrophenyl-alpha-D-glucopyranoside + H2O - Bacillus subtilis D-glucose + p-nitrophenol - ? 214424 3.2.1.10 p-nitrophenyl-alpha-D-glucopyranoside + H2O - Geobacillus stearothermophilus D-glucose + p-nitrophenol - ? 214424 3.2.1.10 p-nitrophenyl-alpha-D-glucopyranoside + H2O - Bacillus cereus D-glucose + p-nitrophenol - ? 214424 3.2.1.10 p-nitrophenyl-alpha-D-glucopyranoside + H2O - Weizmannia coagulans D-glucose + p-nitrophenol - ? 214424 3.2.1.10 p-nitrophenyl-alpha-D-glucopyranoside + H2O - Thermoanaerobium sp. D-glucose + p-nitrophenol - ? 214424 3.2.1.10 p-nitrophenyl-alpha-D-glucopyranoside + H2O - Parageobacillus thermoglucosidasius D-glucose + p-nitrophenol - ? 214424 3.2.1.10 p-nitrophenyl-alpha-D-glucopyranoside + H2O - Bacillus flavocaldarius D-glucose + p-nitrophenol - ? 214424 3.2.1.10 p-nitrophenyl-alpha-D-glucopyranoside + H2O - Bacillus thermoamyloliquefaciens KP1071 D-glucose + p-nitrophenol - ? 214424 3.2.1.10 p-nitrophenyl-alpha-D-glucopyranoside + H2O - Parageobacillus thermoglucosidasius KP 1006 D-glucose + p-nitrophenol - ? 214424 3.2.1.10 p-nitrophenyl-alpha-D-glucopyranoside + H2O - Saccharomyces cerevisiae 4-nitrophenol + D-glucose - ? 259716 3.2.1.10 palatinose + H2O - Homo sapiens D-glucose + fructose - ? 214421 3.2.1.10 palatinose + H2O - Sus scrofa D-glucose + fructose - ? 214421 3.2.1.10 palatinose + H2O - Oryctolagus cuniculus D-glucose + fructose - ? 214421 3.2.1.10 palatinose + H2O - Anser anser D-glucose + fructose - ? 214421 3.2.1.10 palatinose + H2O - Zalophus californianus D-glucose + fructose - ? 214421 3.2.1.10 palatinose + H2O - Callorhinus ursinus D-glucose + fructose - ? 214421 3.2.1.10 palatinose + H2O weak aktivity Thermoanaerobium sp. D-glucose + fructose - ? 214421 3.2.1.10 palatinose + H2O weak aktivity Thermoanaerobium sp. Tok6-B1 D-glucose + fructose - ? 214421 3.2.1.10 palatinose + H2O - Bifidobacterium breve alpha-D-glucose + D-fructose - ? 259707 3.2.1.10 palatinose + H2O - Bifidobacterium breve UCC2003 alpha-D-glucose + D-fructose - ? 259707 3.2.1.10 palatinose + H2O - Saccharomyces cerevisiae D-glucose + D-fructose - ? 430581 3.2.1.10 panitol - Bacillus sp. F5 glucitol + maltitol - ? 214427 3.2.1.10 panose + H2O - Bacillus cereus D-glucose + maltose - ? 94618 3.2.1.10 panose + H2O - Weizmannia coagulans D-glucose + maltose - ? 94618 3.2.1.10 panose + H2O - Thermoanaerobium sp. D-glucose + maltose - ? 94618 3.2.1.10 panose + H2O - Parageobacillus thermoglucosidasius D-glucose + maltose - ? 94618 3.2.1.10 panose + H2O - Bacillus sp. F5 D-glucose + maltose - ? 94618 3.2.1.10 panose + H2O - Thermoanaerobium sp. Tok6-B1 D-glucose + maltose - ? 94618 3.2.1.10 panose + H2O - Parageobacillus thermoglucosidasius KP 1006 D-glucose + maltose - ? 94618 3.2.1.10 panose + H2O alpha-D-Glc-(1->6)-alpha-D-Glc-(1->4)-D-Glc. Multifunctional enzyme that catalyzes also terminal alpha-1,4-linked, alpha-1,2-linked and alpha-1,3-linked glucose residues (EC 3.2.1.20/kojibiose/EC 3.2.1.84 hydrolase) Sulfolobus acidocaldarius maltose + alpha-D-glucose - ? 259704 3.2.1.10 panose + H2O alpha-D-Glc-(1->6)-alpha-D-Glc-(1->4)-D-Glc. Multifunctional enzyme that catalyzes also terminal alpha-1,4-linked, alpha-1,2-linked and alpha-1,3-linked glucose residues (EC 3.2.1.20/kojibiose/EC 3.2.1.84 hydrolase) Sulfolobus acidocaldarius DSM 639 maltose + alpha-D-glucose - ? 259704 3.2.1.10 panose + H2O preference for the cleavage of the alpha-1,6 linkage Bifidobacterium breve alpha-D-glucose + maltose - ? 401620 3.2.1.10 phenyl-alpha-D-glucopyranoside + H2O - Bacillus cereus D-glucose + phenol - ? 214423 3.2.1.10 phenyl-alpha-D-glucopyranoside + H2O - Weizmannia coagulans D-glucose + phenol - ? 214423 3.2.1.10 pullulan + H2O low activity Suncus murinus ? - ? 94190 3.2.1.10 pullulan + H2O - Pisum sativum D-glucose + maltose - ? 214431 3.2.1.10 soluble starch + H2O low activity of wild-type sucrase-isomaltase, very low activity of mutant isomaltase Suncus murinus ? - ? 94576 3.2.1.10 sucrose + H2O - Rattus norvegicus D-fructose + D-glucose - ? 94154 3.2.1.10 sucrose + H2O - Bacillus subtilis D-glucose + D-fructose - ? 94194 3.2.1.10 sucrose + H2O - Homo sapiens D-glucose + D-fructose - ? 94194 3.2.1.10 sucrose + H2O - Weizmannia coagulans D-glucose + D-fructose - ? 94194 3.2.1.10 sucrose + H2O - Saccharomyces cerevisiae D-glucose + D-fructose - ? 94194 3.2.1.10 sucrose + H2O small activity Callorhinus ursinus D-glucose + D-fructose - ? 94194 3.2.1.10 sucrose + H2O wild-type sucrase-isomaltase, no activity with the mutant isomaltase Suncus murinus D-glucose + D-fructose - ? 94194 3.2.1.10 trehalose + H2O - Bacillus sp. (in: Bacteria) ? - ? 94166 3.2.1.10 trehalose + H2O low activity Bifidobacterium adolescentis ? - ? 94166 3.2.1.10 trehalose + H2O - Bacillus sp. (in: Bacteria) SAM1606 ? - ? 94166 3.2.1.10 trehalose + H2O - Bifidobacterium breve 2 alpha-D-glucose - ? 402041 3.2.1.10 trehalulose + H2O - Bifidobacterium breve alpha-D-glucose + beta-D-fructose - ? 402042 3.2.1.10 turanose + H2O - Weizmannia coagulans D-glucose + D-fructose - ? 214429 3.2.1.10 turanose + H2O - Bifidobacterium breve alpha-D-glucose + beta-D-fructose - ? 402073