3.1.31.1 3'-O-acetylnitrophenyl-pdT + H2O - Staphylococcus aureus ? - ? 93513 3.1.31.1 5'-chloromethyl-pdTp-nitrophenyl + H2O - Staphylococcus aureus ? - ? 93520 3.1.31.1 5'-O-acetyl-dTp-nitrophenyl + H2O - Staphylococcus aureus ? - ? 93519 3.1.31.1 5'-sulfate-dTp-nitrophenyl + H2O - Staphylococcus aureus ? - ? 93518 3.1.31.1 DNA - Staphylococcus sp. ? - ? 376743 3.1.31.1 DNA + H2O - Staphylococcus aureus 3'-deoxymononucleotides + dinucleotides - ? 93509 3.1.31.1 DNA + H2O - Staphylococcus sp. 3'-deoxymononucleotides + dinucleotides - ? 93509 3.1.31.1 DNA + H2O - Staphylococcus aureus 3'-deoxymononucleotides + dinucleotides + oligonucleotides terminated by 3'-phosphate, produced only in incomplete digestion ? 93509 3.1.31.1 DNA + H2O in native DNA Xp-dTp and Xp-dAp bonds are preferentially attached in denaturated DNA random cleavage Staphylococcus aureus 3'-deoxymononucleotides + dinucleotides - ? 93509 3.1.31.1 DNA + H2O denaturated DNA is hydrolyzed more rapidly than native DNA Staphylococcus aureus 3'-deoxymononucleotides + dinucleotides - ? 93509 3.1.31.1 DNA + H2O - Staphylococcus aureus V8 3'-deoxymononucleotides + dinucleotides - ? 93509 3.1.31.1 DNA + H2O in native DNA Xp-dTp and Xp-dAp bonds are preferentially attached in denaturated DNA random cleavage Staphylococcus aureus V8 3'-deoxymononucleotides + dinucleotides - ? 93509 3.1.31.1 DNA + H2O denaturated DNA is hydrolyzed more rapidly than native DNA Staphylococcus aureus V8 3'-deoxymononucleotides + dinucleotides - ? 93509 3.1.31.1 DNA + H2O - Staphylococcus aureus KCCM 11335 3'-deoxymononucleotides + dinucleotides - ? 93509 3.1.31.1 DNA + H2O in native DNA Xp-dTp and Xp-dAp bonds are preferentially attached in denaturated DNA random cleavage Staphylococcus aureus Foggi Worthington 3'-deoxymononucleotides + dinucleotides - ? 93509 3.1.31.1 DNA + H2O denaturated DNA is hydrolyzed more rapidly than native DNA Staphylococcus aureus Foggi Worthington 3'-deoxymononucleotides + dinucleotides - ? 93509 3.1.31.1 dTp-nitrophenyl + H2O - Staphylococcus aureus ? - ? 93517 3.1.31.1 GFP-ssDNA + H2O - Plasmodium falciparum ? partial degradation ? 392060 3.1.31.1 GFP-ssRNA + H2O - Plasmodium falciparum ? complete degradation ? 392061 3.1.31.1 M13mp18 DNA + H2O circular single stranded DNA Plasmodium falciparum ? - ? 392520 3.1.31.1 methyl-pdTp-nitrophenyl + H2O - Staphylococcus aureus ? - ? 93516 3.1.31.1 additional information - Staphylococcus aureus ? - ? 89 3.1.31.1 additional information specificity Staphylococcus aureus ? - ? 89 3.1.31.1 additional information inhibition when a 5'-phosphomonoester end group is present in an oligonucleotide Staphylococcus aureus ? - ? 89 3.1.31.1 additional information best substrates oligonucleotides with a 3'-phosphomonoester end group Staphylococcus aureus ? - ? 89 3.1.31.1 additional information substrate masking: binding of RNA by EGTA-inactivated enzyme results in artifactual inhibition of RNA processing Staphylococcus aureus ? - ? 89 3.1.31.1 additional information enzyme does not cleave the 2',3'-cyclic phosphate derivates of the ribonucleosides Staphylococcus aureus ? - ? 89 3.1.31.1 additional information staphylococcal nuclease R, an analogue of the enzyme has the same activity and structural feature as the wild type enzyme Staphylococcus aureus ? - ? 89 3.1.31.1 additional information poly-his-nuclease R can be used both for removal of contaminated DNA and RNA and for separating the enzyme from target proteins Staphylococcus aureus ? - ? 89 3.1.31.1 additional information essential enzyme in the life cycle of Plasmodium falciparum Plasmodium falciparum ? - ? 89 3.1.31.1 additional information GFP-dsDNA and GFP-dsRNA are not used as substrates Plasmodium falciparum ? - ? 89 3.1.31.1 additional information micrococcal nuclease induces double-strand breaks within nucleosome linker regions, and with more extensive digestion, single-strand nicks within the nucleosome itself Staphylococcus aureus ? - ? 89 3.1.31.1 additional information substrate is single-strand salmon sperm DNA Staphylococcus sp. ? - ? 89 3.1.31.1 additional information the enzyme cuts nucleosomal DNA asymmetrically, predominantly in the A/T sequences closest to the nucleosome core/linker junctions. The extent of chromatosomal DNA protected by histone H1 depends on the nucleotide sequence in the linker DNA, overview Staphylococcus aureus ? - ? 89 3.1.31.1 additional information the enzyme possesses calcium-dependent nuclease activity specific to ssRNA, but not dsRNA and DNA Penaeus monodon ? - ? 89 3.1.31.1 additional information enzyme detection based on peptide-bridged energy transfer between mercaptoacetic acid capped CdSe/ZnS quantum dots and dye-labeled ROX-modified 20-mer single-stranded DNA containing AT-rich regions, overview Staphylococcus aureus ? - ? 89 3.1.31.1 additional information isozyme Nuc1 is active with genomic DNA extracted from Staphylococcus aureus, Listeria monocytogenes, and Salmonella, herring sperm DNA, plasmid DNA pNucc from Staphylococcus aureus and pBR122 from Escherichia coli, and RNA from Staphylococcus aureus, substrate specificity of the recombinant nuclease, overview Staphylococcus aureus ? - ? 89 3.1.31.1 additional information isozyme Nuc2 is active with genomic DNA extracted from Staphylococcus aureus, Listeria monocytogenes, and Salmonella, herring sperm DNA, plasmid DNA pNucc from Staphylococcus aureus and pBR122 from Escherichia coli, and RNA from Staphylococcus aureus, substrate specificity of the recombinant nuclease, overview Staphylococcus aureus ? - ? 89 3.1.31.1 additional information label-free and sensitive detection of micrococcal nuclease activity using DNA-scaffolded silver nanoclusters as a fluorescence indicator, evaluation of the quantitative method, overview. The ssDNA is introduced as the enzyme substrate and also as the scaffold for the synthesis of the silver nanoclusters. Since the ssDNA probe P3 acts not only as the substrate for MNase, but also as the scaffold for the silver nanoclusters, the concentration of P3 is obviously a critical factor for the MNase assay. With an increase in P3 concentration, the fluorescence intensity increases either in the presence or absence of the enzyme Staphylococcus aureus ? - ? 89 3.1.31.1 additional information method development for an ultra-high sensitive and selective fluorescent sensing platform for the enzyme based on enzyme-induced DNA strand scission and the difference in affinity of graphene oxide for single-stranded DNA containing different numbers of bases in length, overview. The adsorption of the dye-labeled ssDNA on graphene oxide makes the dyes close proximity to graphene oxide surface resulting in high efficiency quenching of fluorescence of the dyes. Conversely, and very importantly, in the presence of MNase, it cleaves the dye-labeled ssDNA into small fragments. Substrates are commercial and 6-carboxyfluorescein (FAM)-labeled: 20-mer ssDNA with a sequence of 5'-FAM-TATATGGATGATGTGGTATT-3', 10-mer ssDNA with a sequence of 5'FAM-TATATGGATG-3', and 5-mer ssDNA with a sequence of 5'FAM-TATAT-3' Staphylococcus aureus ? - ? 89 3.1.31.1 additional information nucleosomal DNA sizes varying between 147 and 155 bp, the positions of the MNase cuts reflect positions of the A-T pairs rather than the nucleosome core/linker junctions. But a combined treatment with the enzyme and exonuclease III overcomes the enzyme's sequence preference producing nucleosomal DNA trimmed symmetrically and precisely at the core/linker junctions regardless of the underlying DNA sequence, overview. Digestion of the nucleosomes containing CATG tetranucleotide at different positions in relation to the core/linker DNA junction Staphylococcus aureus ? - ? 89 3.1.31.1 additional information substrates are chicken and recombinant frog chromatins, as well as mixture of two plasmid DNAs, one harbouring a 10841-bp segment of sheep DNA containing the beta-lactoglobulin gene and the other harbouring a 13626-bp segment of Saccharomyces cerevisiae DNA incorporating a late-firing replication yeast replication origin, reconstituted with limiting amounts of core histones by salt gradient dialysis. Chromatins, prepared by reconstitution with either chicken or frog histones, are digested to mononucleosomes using micrococcal nuclease, identification of the locations and quantification of the strength of both the chicken or frog histone octamer binding sites on each DNA, the enzyme shows sequence specificity in its preferred cleavage sites with a preference to cut at sites centred on A/T-containing dinucleotides, and comparison to the activity of caspase-activated DNase, overview Staphylococcus aureus ? - ? 89 3.1.31.1 additional information substrates are double-stranded RNA targeting PAZ domain of PmAgo1, PmRab7, and gfp. The enzyme shows calcium-dependent RNase activity, overview Penaeus monodon ? - ? 89 3.1.31.1 additional information the wild-type and truncated mutant isozyme Nuc2 degrades several nucleotide substrates, including Staphylococcus aureus genomic DNA, eukaryotic salmon sperm DNA, double-stranded plasmid DNA, and single-stranded DNA Staphylococcus aureus ? - ? 89 3.1.31.1 additional information the wild-type and truncated mutant isozyme Nuc2 degrades several nucleotide substrates, including Staphylococcus aureus genomic DNA, eukaryotic salmon sperm DNA, double-stranded plasmid DNA, and single-stranded DNA Staphylococcus aureus N315 ? - ? 89 3.1.31.1 additional information isozyme Nuc1 is active with genomic DNA extracted from Staphylococcus aureus, Listeria monocytogenes, and Salmonella, herring sperm DNA, plasmid DNA pNucc from Staphylococcus aureus and pBR122 from Escherichia coli, and RNA from Staphylococcus aureus, substrate specificity of the recombinant nuclease, overview Staphylococcus aureus N315 ? - ? 89 3.1.31.1 additional information method development for an ultra-high sensitive and selective fluorescent sensing platform for the enzyme based on enzyme-induced DNA strand scission and the difference in affinity of graphene oxide for single-stranded DNA containing different numbers of bases in length, overview. The adsorption of the dye-labeled ssDNA on graphene oxide makes the dyes close proximity to graphene oxide surface resulting in high efficiency quenching of fluorescence of the dyes. Conversely, and very importantly, in the presence of MNase, it cleaves the dye-labeled ssDNA into small fragments. Substrates are commercial and 6-carboxyfluorescein (FAM)-labeled: 20-mer ssDNA with a sequence of 5'-FAM-TATATGGATGATGTGGTATT-3', 10-mer ssDNA with a sequence of 5'FAM-TATATGGATG-3', and 5-mer ssDNA with a sequence of 5'FAM-TATAT-3' Staphylococcus aureus CCTCC AB91093 ? - ? 89 3.1.31.1 additional information specificity Staphylococcus aureus V8 ? - ? 89 3.1.31.1 additional information specificity Staphylococcus aureus Foggi Worthington ? - ? 89 3.1.31.1 nitrophenyl-pdT + H2O - Staphylococcus aureus ? - ? 93512 3.1.31.1 nitrophenyl-pdT + H2O - Staphylococcus aureus V8 ? - ? 93512 3.1.31.1 nitrophenyl-pdT + H2O - Staphylococcus aureus Foggi Worthington ? - ? 93512 3.1.31.1 nitrophenyl-pdTp + H2O - Staphylococcus aureus ? - ? 93514 3.1.31.1 nitrophenyl-pdTpdTp-nitrophenyl + H2O - Staphylococcus aureus ? - ? 93515 3.1.31.1 RNA + H2O - Staphylococcus aureus nucleoside 3'-phosphates + dinucleotides - ? 93511 3.1.31.1 RNA + H2O - Staphylococcus aureus nucleoside 3'-phosphates + dinucleotides dinucleotides terminated by 3'-phosphates ? 93511 3.1.31.1 RNA + H2O - Staphylococcus aureus nucleoside 3'-phosphates + dinucleotides nucleoside 3'-phosphates of both purines and pyrimidines ? 93511 3.1.31.1 RNA + H2O - Staphylococcus aureus V8 nucleoside 3'-phosphates + dinucleotides - ? 93511 3.1.31.1 RNA + H2O - Staphylococcus aureus Foggi Worthington nucleoside 3'-phosphates + dinucleotides - ? 93511 3.1.31.1 RNA + H2O the enzyme cleaves RNA chains at the 5'-side of the phosphodiester linkage to produce degraded fragments with 5'-hydroxyl and 3'-phosphate ends. cTSN degrades single-stranded RNA and double-stranded RNA containing mismatched base pairs, but is not restricted to those containing multiple I/U and U/I pairs. Tudor staphylococcal nuclease is a structure-specific ribonuclease targeting single-stranded RNA and unstructured regions of double-stranded RNA Caenorhabditis elegans ? - ? 141227 3.1.31.1 ss-DNA + H2O single strand salmon sperm DNA, obtained by boiling for 30 min and rapid cooling on ice Staphylococcus aureus ? - ? 393534 3.1.31.1 ssDNA + H2O single stranded salmon sperm DNA Staphylococcus aureus ? - ? 364420 3.1.31.1 T2 DNA + H2O - Staphylococcus aureus ? - ? 93510