3.1.3.8 1-L-myo-inositol 1,2,3,4,5-pentakisphosphate + H2O - Pantoea agglomerans ? - ? 380847 3.1.3.8 1-L-myo-inositol 1,2,3,5,6-pentakisphosphate + H2O - Pantoea agglomerans ? - ? 380848 3.1.3.8 1-naphthyl phosphate + H2O - Aspergillus niger 1-naphthol + phosphate - ? 73717 3.1.3.8 1-naphthyl phosphate + H2O - Lupinus albus 1-naphthol + phosphate - ? 73717 3.1.3.8 1-naphthyl phosphate + H2O - Raoultella terrigena 1-naphthol + phosphate - ? 73717 3.1.3.8 1-naphthyl phosphate + H2O 130% of the activity with myo-inositol hexakisphosphate Corylus avellana 1-naphthol + phosphate - ? 73717 3.1.3.8 1-naphthyl phosphate + H2O 2.1% of the activity with myo-inositol hexakisphosphate, recombinant enzyme Klebsiella sp. 1-naphthol + phosphate - ? 73717 3.1.3.8 1-naphthyl phosphate + H2O 74% of the activity with myo-inositol hexakisphosphate Aspergillus niger 1-naphthol + phosphate - ? 73717 3.1.3.8 1-naphthyl phosphate + H2O 89.6% of the activity with myo-inositol hexakisphosphate Rhizopus microsporus var. oligosporus 1-naphthol + phosphate - ? 73717 3.1.3.8 1-naphthyl phosphate + H2O - Aspergillus niger FS3 1-naphthol + phosphate - ? 73717 3.1.3.8 2-glycerophosphate + H2O - Lupinus albus glycerate + phosphate - ? 364551 3.1.3.8 2-glycerophosphate + H2O 19.8% of the activity with myo-inositol hexakisphosphate, recombinant enzyme Klebsiella sp. glycerate + phosphate - ? 364551 3.1.3.8 2-glycerophosphate + H2O 127% of the activity with myo-inositol hexakisphosphate Aspergillus ficuum glycerate + phosphate - ? 364551 3.1.3.8 2-naphthyl phosphate + H2O - Lupinus albus 2-naphthol + phosphate - ? 73554 3.1.3.8 2-naphthyl phosphate + H2O - Raoultella terrigena 2-naphthol + phosphate - ? 73554 3.1.3.8 2-naphthyl phosphate + H2O 4.9% of the activity with myo-inositol hexakisphosphate, recombinant enzyme Klebsiella sp. 2-naphthol + phosphate - ? 73554 3.1.3.8 2-naphthyl phosphate + H2O 63.6% of the activity with myo-inositol hexakisphosphate Aspergillus niger 2-naphthol + phosphate - ? 73554 3.1.3.8 2-naphthyl phosphate + H2O 97.1% of the activity with myo-inositol hexakisphosphate Rhizopus microsporus var. oligosporus 2-naphthol + phosphate - ? 73554 3.1.3.8 2-phosphoglycerate + H2O - Aspergillus niger glyceric acid + phosphate - ? 83148 3.1.3.8 3-phosphoglycerate + H2O - Aspergillus fumigatus glycerate + phosphate - ? 64594 3.1.3.8 3-phosphoglycerate + H2O 168% relative activity compared to myo-inositol hexakisphosphate Nicotiana tabacum glycerate + phosphate - ? 64594 3.1.3.8 3-phosphoglycerate + H2O 95% activity compared to myo-inositol hexakisphosphate Debaryomyces castellii glycerate + phosphate - ? 64594 3.1.3.8 3-phosphoglycerate + H2O 69% of the activity with 4-nitrophenylphosphate Arabidopsis thaliana glycerate + phosphate - ? 64594 3.1.3.8 3-phosphoglycerate + H2O 95% activity compared to myo-inositol hexakisphosphate Debaryomyces castellii CBS 2923 glycerate + phosphate - ? 64594 3.1.3.8 4-methylumbelliferyl phosphate + H2O phytase activity detection using 4-methylumbelliferyl phosphate as substrate Yersinia mollaretii 4-methylumbelliferone + phosphate - ? 73561 3.1.3.8 4-methylumbelliferyl phosphate + H2O phytase activity detection using 4-methylumbelliferyl phosphate as substrate Yersinia mollaretii CNY 7263 4-methylumbelliferone + phosphate - ? 73561 3.1.3.8 4-methylumbelliferyl phosphate + H2O phytase activity detection using 4-methylumbelliferyl phosphate as substrate Yersinia mollaretii DSM 18520 4-methylumbelliferone + phosphate - ? 73561 3.1.3.8 4-methylumbelliferyl phosphate + H2O phytase activity detection using 4-methylumbelliferyl phosphate as substrate Yersinia mollaretii CIP 103324 4-methylumbelliferone + phosphate - ? 73561 3.1.3.8 4-methylumbelliferyl phosphate + H2O phytase activity detection using 4-methylumbelliferyl phosphate as substrate Yersinia mollaretii ATCC 43969 4-methylumbelliferone + phosphate - ? 73561 3.1.3.8 4-methylumbelliferyl phosphate + H2O phytase activity detection using 4-methylumbelliferyl phosphate as substrate Yersinia mollaretii WAIP 204 4-methylumbelliferone + phosphate - ? 73561 3.1.3.8 4-nitrophenyl phosphate + H2O - Aspergillus niger 4-nitrophenol + phosphate - ? 73771 3.1.3.8 4-nitrophenyl phosphate + H2O - Glycine max 4-nitrophenol + phosphate - ? 73771 3.1.3.8 4-nitrophenyl phosphate + H2O - Lupinus albus 4-nitrophenol + phosphate - ? 73771 3.1.3.8 4-nitrophenyl phosphate + H2O - Bacillus sp. (in: Bacteria) 4-nitrophenol + phosphate - ? 73771 3.1.3.8 4-nitrophenyl phosphate + H2O - Aspergillus terreus 4-nitrophenol + phosphate - ? 73771 3.1.3.8 4-nitrophenyl phosphate + H2O - Raoultella terrigena 4-nitrophenol + phosphate - ? 73771 3.1.3.8 4-nitrophenyl phosphate + H2O - Aspergillus fumigatus 4-nitrophenol + phosphate - ? 73771 3.1.3.8 4-nitrophenyl phosphate + H2O - Aspergillus ficuum 4-nitrophenol + phosphate - ? 73771 3.1.3.8 4-nitrophenyl phosphate + H2O - Penicillium parvum 4-nitrophenol + phosphate - ? 73771 3.1.3.8 4-nitrophenyl phosphate + H2O - Rhizopus microsporus 4-nitrophenol + phosphate - ? 73771 3.1.3.8 4-nitrophenyl phosphate + H2O - Lilium longiflorum 4-nitrophenol + phosphate - ? 73771 3.1.3.8 4-nitrophenyl phosphate + H2O less than 10% of the activity with myo-inositol hexakisphosphate Klebsiella oxytoca 4-nitrophenol + phosphate - ? 73771 3.1.3.8 4-nitrophenyl phosphate + H2O 7.6% of the activity with myo-inositol hexakisphosphate Klebsiella sp. 4-nitrophenol + phosphate - ? 73771 3.1.3.8 4-nitrophenyl phosphate + H2O 101% of the activity with myo-inositol hexakisphosphate Corylus avellana 4-nitrophenol + phosphate - ? 73771 3.1.3.8 4-nitrophenyl phosphate + H2O 11% of the activity with myo-inositol hexakisphosphate Citrobacter braakii 4-nitrophenol + phosphate - ? 73771 3.1.3.8 4-nitrophenyl phosphate + H2O 23.7% of the activity with myo-inositol hexakisphosphate, recombinant enzyme Klebsiella sp. 4-nitrophenol + phosphate - ? 73771 3.1.3.8 4-nitrophenyl phosphate + H2O 39.8% of the activity with myo-inositol hexakisphosphate Aspergillus niger 4-nitrophenol + phosphate - ? 73771 3.1.3.8 4-nitrophenyl phosphate + H2O 59.6% of the activity with myo-inositol hexakisphosphate Rhizopus microsporus var. oligosporus 4-nitrophenol + phosphate - ? 73771 3.1.3.8 4-nitrophenyl phosphate + H2O reaction is nearly as efficient as on phytate Wickerhamomyces anomalus 4-nitrophenol + phosphate - ? 73771 3.1.3.8 4-nitrophenyl phosphate + H2O The enzyme may be a 3-phytase, EC 3.1.3.8, or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate (3-phytase) or 1D-myo-inositol 1,2,3,5,6-pentakisphosphate (4-phytase) (i.e. 1L-myo-inositol 1,2,3,4,5-pentakisphosphate if 1L numbering is applied) has not been analyzed. The reaction was monitored by analyzing the released phosphate Aspergillus niger 4-nitrophenol + phosphate - ? 73771 3.1.3.8 4-nitrophenyl phosphate + H2O 0.65% of the activity with myo-inositol hexakisphosphate Pectobacterium carotovorum 4-nitrophenol + phosphate - ? 73771 3.1.3.8 4-nitrophenyl phosphate + H2O 254% of the activity with myo-inositol hexakisphosphate Aspergillus ficuum 4-nitrophenol + phosphate - ? 73771 3.1.3.8 4-nitrophenyl phosphate + H2O - Penicillium parvum BCC1769 4-nitrophenol + phosphate - ? 73771 3.1.3.8 4-nitrophenyl phosphate + H2O 254% of the activity with myo-inositol hexakisphosphate Aspergillus ficuum NTG-23 4-nitrophenol + phosphate - ? 73771 3.1.3.8 4-nitrophenyl phosphate + H2O - Aspergillus terreus 9A1 4-nitrophenol + phosphate - ? 73771 3.1.3.8 4-nitrophenyl phosphate + H2O - Bacillus sp. (in: Bacteria) MD2 4-nitrophenol + phosphate - ? 73771 3.1.3.8 4-nitrophenyl phosphate + H2O - Aspergillus niger FS3 4-nitrophenol + phosphate - ? 73771 3.1.3.8 4-nitrophenyl phosphate + H2O 7.6% of the activity with myo-inositol hexakisphosphate Klebsiella sp. PG-2 4-nitrophenol + phosphate - ? 73771 3.1.3.8 4-nitrophenyl phosphate + H2O - Aspergillus ficuum NRRL 3135 4-nitrophenol + phosphate - ? 73771 3.1.3.8 4-nitrophenylphosphate + H2O - Arabidopsis thaliana 4-nitrophenol + phosphate - ? 64612 3.1.3.8 4-nitrophenylphosphate + H2O - Aspergillus fumigatus 4-nitrophenol + phosphate - ? 64612 3.1.3.8 5'-ATP + H2O 250% of the activity with myo-inositol hexakisphosphate Corylus avellana ? - ? 73513 3.1.3.8 6-phosphogluconate + H2O - Aspergillus niger gluconate + phosphate - ? 83154 3.1.3.8 ADP + H2O - Aspergillus niger ? - ? 64621 3.1.3.8 ADP + H2O - Lupinus albus ? - ? 64621 3.1.3.8 ADP + H2O - Raoultella terrigena ? - ? 64621 3.1.3.8 ADP + H2O 75% of the activity with myo-inositol hexakisphosphate Bacillus subtilis ? - ? 64621 3.1.3.8 ADP + H2O 14.2% of the activity with myo-inositol hexakisphosphate Rhizopus microsporus var. oligosporus ? - ? 64621 3.1.3.8 ADP + H2O 25.6% of the activity with myo-inositol hexakisphosphate Aspergillus niger ? - ? 64621 3.1.3.8 ADP + H2O 7.5% of the activity with myo-inositol hexakisphosphate, recombinant enzyme Klebsiella sp. ? - ? 64621 3.1.3.8 ADP + H2O 75% of the activity with myo-inositol hexakisphosphate Bacillus subtilis VTT E-68013 ? - ? 64621 3.1.3.8 ADP + H2O 7.5% of the activity with myo-inositol hexakisphosphate, recombinant enzyme Klebsiella sp. ASR1 ? - ? 64621 3.1.3.8 ADP + H2O - Aspergillus niger AMP + phosphate - ? 93189 3.1.3.8 ADP + H2O 120% activity compared to myo-inositol hexakisphosphate Debaryomyces castellii AMP + phosphate - ? 93189 3.1.3.8 ADP + H2O 206% relative activity compared to myo-inositol hexakisphosphate Nicotiana tabacum AMP + phosphate - ? 93189 3.1.3.8 ADP + H2O - Aspergillus niger FS3 AMP + phosphate - ? 93189 3.1.3.8 ADP + H2O 130% of the activity with myo-inositol hexakisphosphate Volvariella volvacea ? + phosphate - ? 391192 3.1.3.8 ADP + H2O 0.02% of the activity with myo-inositol hexakisphosphate Pectobacterium carotovorum ? + phosphate - ? 391192 3.1.3.8 ADP + H2O 177% of the activity with myo-inositol hexakisphosphate Aspergillus ficuum ? + phosphate - ? 391192 3.1.3.8 ADP + H2O 50% of the activity with 4-nitrophenylphosphate Arabidopsis thaliana ? + phosphate - ? 391192 3.1.3.8 ADP + H2O 80% of the activity with myo-inositol hexakisphosphate Bacillus subtilis ? + phosphate - ? 391192 3.1.3.8 ADP + H2O 103% of the activity with myo-inositol hexakisphosphate Lentinula edodes ? + phosphate - ? 391192 3.1.3.8 ADP + H2O 80% of the activity with myo-inositol hexakisphosphate Bacillus subtilis US417 ? + phosphate - ? 391192 3.1.3.8 alpha-glycerophosphate + H2O 78% activity compared to myo-inositol hexakisphosphate Debaryomyces castellii glycerol + phosphate - ? 83094 3.1.3.8 AMP + H2O - Lupinus albus adenosine + phosphate - ? 73505 3.1.3.8 AMP + H2O - Raoultella terrigena adenosine + phosphate - ? 73505 3.1.3.8 AMP + H2O no activity Aspergillus niger adenosine + phosphate - ? 73505 3.1.3.8 AMP + H2O less than 10% of the activity with myo-inositol hexakisphosphate Klebsiella oxytoca adenosine + phosphate - ? 73505 3.1.3.8 AMP + H2O 25.0% of the activity with myo-inositol hexakisphosphate Rhizopus microsporus var. oligosporus adenosine + phosphate - ? 73505 3.1.3.8 AMP + H2O 9.6% of the activity with myo-inositol hexakisphosphate, recombinant enzyme Klebsiella sp. adenosine + phosphate - ? 73505 3.1.3.8 AMP + H2O 30% relative activity compared to myo-inositol hexakisphosphate Nicotiana tabacum adenosine + phosphate - ? 73505 3.1.3.8 AMP + H2O 50% activity compared to myo-inositol hexakisphosphate Debaryomyces castellii adenosine + phosphate - ? 73505 3.1.3.8 AMP + H2O 10% of the activity with myo-inositol hexakisphosphate Bacillus subtilis adenosine + phosphate - ? 73505 3.1.3.8 AMP + H2O 173% of the activity with myo-inositol hexakisphosphate Aspergillus ficuum adenosine + phosphate - ? 73505 3.1.3.8 AMP + H2O 143% of the activity with myo-inositol hexakisphosphate Lentinula edodes adenosine + phosphate - ? 73505 3.1.3.8 AMP + H2O 195% of the activity with myo-inositol hexakisphosphate Volvariella volvacea adenosine + phosphate - ? 73505 3.1.3.8 AMP + H2O 50% activity compared to myo-inositol hexakisphosphate Debaryomyces castellii CBS 2923 adenosine + phosphate - ? 73505 3.1.3.8 AMP + H2O 173% of the activity with myo-inositol hexakisphosphate Aspergillus ficuum NTG-23 adenosine + phosphate - ? 73505 3.1.3.8 AMP + H2O 10% of the activity with myo-inositol hexakisphosphate Bacillus subtilis US417 adenosine + phosphate - ? 73505 3.1.3.8 AMP + H2O less than 10% of the activity with myo-inositol hexakisphosphate Klebsiella oxytoca MO-3 adenosine + phosphate - ? 73505 3.1.3.8 AMP + phosphate 43.9% of the activity with myo-inositol hexakisphosphate Aspergillus niger adenosine + phosphate - ? 364546 3.1.3.8 ATP + H2O - Aspergillus niger ? - ? 64622 3.1.3.8 ATP + H2O - Lupinus albus ? - ? 64622 3.1.3.8 ATP + H2O - Raoultella terrigena ? - ? 64622 3.1.3.8 ATP + H2O - Aspergillus fumigatus ? - ? 64622 3.1.3.8 ATP + H2O - Aspergillus ficuum ? - ? 64622 3.1.3.8 ATP + H2O less than 10% of the activity with myo-inositol hexakisphosphate Klebsiella oxytoca ? - ? 64622 3.1.3.8 ATP + H2O 50% of the activity with myo-inositol hexakisphosphate Bacillus subtilis ? - ? 64622 3.1.3.8 ATP + H2O 22.3% of the activity with myo-inositol hexakisphosphate Rhizopus microsporus var. oligosporus ? - ? 64622 3.1.3.8 ATP + H2O 38.8% of the activity with myo-inositol hexakisphosphate Aspergillus niger ? - ? 64622 3.1.3.8 ATP + H2O 5.6% of the activity with myo-inositol hexakisphosphate, recombinant enzyme Klebsiella sp. ? - ? 64622 3.1.3.8 ATP + H2O reaction is nearly as efficient as on phytate Wickerhamomyces anomalus ? - ? 64622 3.1.3.8 ATP + H2O 50% of the activity with myo-inositol hexakisphosphate Bacillus subtilis VTT E-68013 ? - ? 64622 3.1.3.8 ATP + H2O less than 10% of the activity with myo-inositol hexakisphosphate Klebsiella oxytoca MO-3 ? - ? 64622 3.1.3.8 ATP + H2O - Aspergillus niger ADP + phosphate - ? 92967 3.1.3.8 ATP + H2O - Glycine max ADP + phosphate - ? 92967 3.1.3.8 ATP + H2O 133% activity compared to myo-inositol hexakisphosphate Debaryomyces castellii ADP + phosphate - ? 92967 3.1.3.8 ATP + H2O 365% relative activity compared to myo-inositol hexakisphosphate Nicotiana tabacum ADP + phosphate - ? 92967 3.1.3.8 ATP + H2O 76.67% activity compared to myo-inositol hexakisphosphate Papiliotrema laurentii ADP + phosphate - ? 92967 3.1.3.8 ATP + H2O 76.67% activity compared to myo-inositol hexakisphosphate Papiliotrema laurentii ABO 510 ADP + phosphate - ? 92967 3.1.3.8 ATP + H2O - Aspergillus niger FS3 ADP + phosphate - ? 92967 3.1.3.8 ATP + H2O 0.26% of the activity with myo-inositol hexakisphosphate Pectobacterium carotovorum ? + phosphate - ? 391344 3.1.3.8 ATP + H2O 256% of the activity with myo-inositol hexakisphosphate Aspergillus ficuum ? + phosphate - ? 391344 3.1.3.8 ATP + H2O 256% of the activity with myo-inositol hexakisphosphate Volvariella volvacea ? + phosphate - ? 391344 3.1.3.8 ATP + H2O 97% of the activity with myo-inositol hexakisphosphate Bacillus subtilis ? + phosphate - ? 391344 3.1.3.8 ATP + H2O 208% of the activity with myo-inositol hexakisphosphate Lentinula edodes ? + phosphate - ? 391344 3.1.3.8 ATP + H2O 97% of the activity with myo-inositol hexakisphosphate Bacillus subtilis US417 ? + phosphate - ? 391344 3.1.3.8 beta-glycerophosphate + H2O - Aspergillus fumigatus glycerol + phosphate - ? 64615 3.1.3.8 CTP + H2O 352% relative activity compared to myo-inositol hexakisphosphate Nicotiana tabacum CDP + phosphate - ? 93269 3.1.3.8 D-fructose 1,6-bisphosphate + H2O - Pantoea agglomerans ? - ? 364519 3.1.3.8 D-fructose 1,6-diphosphate + H2O - Lupinus albus ? - ? 73630 3.1.3.8 D-fructose 1,6-diphosphate + H2O 146% of the activity with myo-inositol hexakisphosphate Corylus avellana ? - ? 73630 3.1.3.8 D-fructose 1,6-diphosphate + H2O 202.19% activity compared to myo-inositol hexakisphosphate Papiliotrema laurentii ? - ? 73630 3.1.3.8 D-fructose 1,6-diphosphate + H2O 202.19% activity compared to myo-inositol hexakisphosphate Papiliotrema laurentii ABO 510 ? - ? 73630 3.1.3.8 D-fructose 1-phosphate + H2O - Pantoea agglomerans D-fructose + phosphate - ? 73624 3.1.3.8 D-fructose 1-phosphate + H2O poor substrate Obesumbacterium proteus D-fructose + phosphate - ? 73624 3.1.3.8 D-fructose 6-phosphate + H2O - Aspergillus niger D-fructose + phosphate - ? 13177 3.1.3.8 D-fructose 6-phosphate + H2O - Raoultella terrigena D-fructose + phosphate - ? 13177 3.1.3.8 D-fructose 6-phosphate + H2O 8.1% of the activity with myo-inositol hexakisphosphate, recombinant enzyme Klebsiella sp. D-fructose + phosphate - ? 13177 3.1.3.8 D-fructose 6-phosphate + H2O 35% activity compared to myo-inositol hexakisphosphate Debaryomyces castellii D-fructose + phosphate - ? 13177 3.1.3.8 D-fructose 6-phosphate + H2O 75% relative activity compared to myo-inositol hexakisphosphate Nicotiana tabacum D-fructose + phosphate - ? 13177 3.1.3.8 D-fructose 6-phosphate + H2O 228% of the activity with myo-inositol hexakisphosphate Aspergillus ficuum D-fructose + phosphate - ? 13177 3.1.3.8 D-fructose 6-phosphate + H2O 158% of the activity with myo-inositol hexakisphosphate Lentinula edodes D-fructose + phosphate - ? 13177 3.1.3.8 D-fructose 6-phosphate + H2O 229% of the activity with myo-inositol hexakisphosphate Volvariella volvacea D-fructose + phosphate - ? 13177 3.1.3.8 D-fructose 6-phosphate + H2O 35% activity compared to myo-inositol hexakisphosphate Debaryomyces castellii CBS 2923 D-fructose + phosphate - ? 13177 3.1.3.8 D-fructose 6-phosphate + H2O 228% of the activity with myo-inositol hexakisphosphate Aspergillus ficuum NTG-23 D-fructose + phosphate - ? 13177 3.1.3.8 D-fructose 6-phosphate + H2O 8.1% of the activity with myo-inositol hexakisphosphate, recombinant enzyme Klebsiella sp. ASR1 D-fructose + phosphate - ? 13177 3.1.3.8 D-fructose 6-phosphate + H2O - Lupinus albus fructose + phosphate - ? 364598 3.1.3.8 D-glucose 1-phosphate + H2O - Pantoea agglomerans D-glucose + phosphate - ? 93177 3.1.3.8 D-glucose 1-phosphate + H2O poor substrate Obesumbacterium proteus D-glucose + phosphate - ? 93177 3.1.3.8 D-glucose 1-phosphate + H2O 28.6% of the activity with myo-inositol hexakisphosphate Aspergillus niger D-glucose + phosphate - ? 93177 3.1.3.8 D-glucose 1-phosphate + H2O 92.0% of the activity with myo-inositol hexakisphosphate Rhizopus microsporus var. oligosporus D-glucose + phosphate - ? 93177 3.1.3.8 D-glucose 1-phosphate + H2O strong preference for glucose 1-phosphate over phytate, under physiological conditions glucose 1-phosphate is its most likely substrate Pantoea agglomerans D-glucose + phosphate - ? 93177 3.1.3.8 D-glucose 1-phosphate + H2O 5% relative activity compared to myo-inositol hexakisphosphate Nicotiana tabacum D-glucose + phosphate - ? 93177 3.1.3.8 D-glucose 1-phosphate + H2O 76.72% activity compared to myo-inositol hexakisphosphate Papiliotrema laurentii D-glucose + phosphate - ? 93177 3.1.3.8 D-glucose 3-phosphate + H2O 75% relative activity compared to myo-inositol hexakisphosphate Nicotiana tabacum glucose + phosphate - ? 391748 3.1.3.8 D-glucose 6-phosphate + H2O - Pantoea agglomerans D-glucose + phosphate - ? 73625 3.1.3.8 D-glucose 6-phosphate + H2O - Lupinus albus D-glucose + phosphate - ? 73625 3.1.3.8 D-glucose 6-phosphate + H2O poor substrate Obesumbacterium proteus D-glucose + phosphate - ? 73625 3.1.3.8 D-glucose 6-phosphate + H2O 78.5% of the activity with myo-inositol hexakisphosphate Corylus avellana D-glucose + phosphate - ? 73625 3.1.3.8 D-glucose 6-phosphate + H2O 57% relative activity compared to myo-inositol hexakisphosphate Nicotiana tabacum D-glucose + phosphate - ? 73625 3.1.3.8 D-glucose 6-phosphate + H2O 73.75% activity compared to myo-inositol hexakisphosphate Papiliotrema laurentii D-glucose + phosphate - ? 73625 3.1.3.8 D-glucose 6-phosphate + H2O 82% activity compared to myo-inositol hexakisphosphate Debaryomyces castellii D-glucose + phosphate - ? 73625 3.1.3.8 D-glucose 6-phosphate + H2O 0.39% of the activity with myo-inositol hexakisphosphate Pectobacterium carotovorum D-glucose + phosphate - ? 73625 3.1.3.8 D-glucose 6-phosphate + H2O 190% of the activity with myo-inositol hexakisphosphate Aspergillus ficuum D-glucose + phosphate - ? 73625 3.1.3.8 D-glucose 6-phosphate + H2O 20% of the activity with 4-nitrophenylphosphate Arabidopsis thaliana D-glucose + phosphate - ? 73625 3.1.3.8 D-glucose 6-phosphate + H2O 128% of the activity with myo-inositol hexakisphosphate Lentinula edodes D-glucose + phosphate - ? 73625 3.1.3.8 D-glucose 6-phosphate + H2O 217% of the activity with myo-inositol hexakisphosphate Volvariella volvacea D-glucose + phosphate - ? 73625 3.1.3.8 D-glucose 6-phosphate + H2O 73.75% activity compared to myo-inositol hexakisphosphate Papiliotrema laurentii ABO 510 D-glucose + phosphate - ? 73625 3.1.3.8 D-glucose 6-phosphate + H2O 190% of the activity with myo-inositol hexakisphosphate Aspergillus ficuum NTG-23 D-glucose + phosphate - ? 73625 3.1.3.8 D-glucose 6-phosphate + H2O reaction is nearly as efficient as on phytate Wickerhamomyces anomalus glucose + phosphate - ? 364548 3.1.3.8 D-ribose 5-phosphate + H2O - Aspergillus niger D-ribose + phosphate - ? 13179 3.1.3.8 D-ribose 5-phosphate + H2O poor substrate Obesumbacterium proteus D-ribose + phosphate - ? 13179 3.1.3.8 D-ribose 5-phosphate + H2O 50% of the activity with myo-inositol hexakisphosphate Corylus avellana D-ribose + phosphate - ? 13179 3.1.3.8 D-ribose 5-phosphate + H2O 75% relative activity compared to myo-inositol hexakisphosphate Nicotiana tabacum D-ribose + phosphate - ? 13179 3.1.3.8 D-ribose 5-phosphate + H2O - Pantoea agglomerans ? - ? 83126 3.1.3.8 dATP + H2O 338% relative activity compared to myo-inositol hexakisphosphate Nicotiana tabacum dADP + phosphate - ? 260008 3.1.3.8 dATP + H2O 31% of the activity with 4-nitrophenylphosphate Arabidopsis thaliana ? + phosphate - ? 411970 3.1.3.8 dCTP + H2O 367% relative activity compared to myo-inositol hexakisphosphate Nicotiana tabacum dCDP + phosphate - ? 141341 3.1.3.8 dCTP + H2O 35% of the activity with 4-nitrophenylphosphate Arabidopsis thaliana ? + phosphate - ? 411972 3.1.3.8 dGTP + H2O 189% relative activity compared to myo-inositol hexakisphosphate Nicotiana tabacum dGDP + phosphate - ? 373042 3.1.3.8 dGTP + H2O 52% of the activity with 4-nitrophenylphosphate Arabidopsis thaliana ? + phosphate - ? 411979 3.1.3.8 di-sodium phenyl phosphate dihydrate + H2O 111.85% activity compared to myo-inositol hexakisphosphate Papiliotrema laurentii ? - ? 391825 3.1.3.8 diphosphate + H2O - Lupinus albus 2 phosphate - ? 73540 3.1.3.8 diphosphate + H2O - Raoultella terrigena 2 phosphate - ? 73540 3.1.3.8 diphosphate + H2O - Aspergillus ficuum 2 phosphate - ? 73540 3.1.3.8 diphosphate + H2O less than 10% of the activity with myo-inositol hexakisphosphate Klebsiella oxytoca 2 phosphate - ? 73540 3.1.3.8 diphosphate + H2O 438.5% of the activity with myo-inositol hexakisphosphate Corylus avellana 2 phosphate - ? 73540 3.1.3.8 diphosphate + H2O 95% of the activity with 4-nitrophenylphosphate Arabidopsis thaliana 2 phosphate - ? 73540 3.1.3.8 diphosphate + H2O Na-diphosphate Bacillus sp. (in: Bacteria) 2 phosphate - ? 73540 3.1.3.8 diphosphate + H2O Na-diphosphate Bacillus sp. (in: Bacteria) MD2 2 phosphate - ? 73540 3.1.3.8 diphosphate + H2O less than 10% of the activity with myo-inositol hexakisphosphate Klebsiella oxytoca MO-3 2 phosphate - ? 73540 3.1.3.8 dTTP + H2O 389% relative activity compared to myo-inositol hexakisphosphate Nicotiana tabacum dTDP + phosphate - ? 141339 3.1.3.8 dTTP + H2O 37% of the activity with 4-nitrophenylphosphate Arabidopsis thaliana ? + phosphate - ? 412014 3.1.3.8 fructose 1,6-bisphosphate + H2O - Aspergillus fumigatus ? - ? 360957 3.1.3.8 fructose 1,6-diphosphate + H2O - Aspergillus niger ? - ? 64598 3.1.3.8 fructose 1,6-diphosphate + H2O - Raoultella terrigena ? - ? 64598 3.1.3.8 fructose 1,6-diphosphate + H2O - Aspergillus ficuum ? - ? 64598 3.1.3.8 fructose 1,6-diphosphate + H2O 12.3% of the activity with myo-inositol hexakisphosphate, recombinant enzyme Klebsiella sp. ? - ? 64598 3.1.3.8 fructose 1,6-diphosphate + H2O 12.3% of the activity with myo-inositol hexakisphosphate, recombinant enzyme Klebsiella sp. ASR1 ? - ? 64598 3.1.3.8 glucose 1-phosphate + H2O - Aspergillus niger glucose + phosphate - ? 73546 3.1.3.8 Glucose 6-phosphate + H2O - Aspergillus niger Glucose + phosphate - ? 13176 3.1.3.8 Glucose 6-phosphate + H2O - Raoultella terrigena Glucose + phosphate - ? 13176 3.1.3.8 Glucose 6-phosphate + H2O - Aspergillus ficuum Glucose + phosphate - ? 13176 3.1.3.8 Glucose 6-phosphate + H2O less than 10% of the activity with myo-inositol hexakisphosphate Klebsiella oxytoca Glucose + phosphate - ? 13176 3.1.3.8 Glucose 6-phosphate + H2O 2.7% of the activity with myo-inositol hexakisphosphate, recombinant enzyme Klebsiella sp. Glucose + phosphate - ? 13176 3.1.3.8 Glucose 6-phosphate + H2O 2.7% of the activity with myo-inositol hexakisphosphate, recombinant enzyme Klebsiella sp. ASR1 Glucose + phosphate - ? 13176 3.1.3.8 Glucose 6-phosphate + H2O less than 10% of the activity with myo-inositol hexakisphosphate Klebsiella oxytoca MO-3 Glucose + phosphate - ? 13176 3.1.3.8 glycerol 2-phosphate + H2O - Aspergillus niger glycerol + phosphate - ? 73732 3.1.3.8 glycerol 2-phosphate + H2O - Raoultella terrigena glycerol + phosphate - ? 73732 3.1.3.8 glycerol 2-phosphate + H2O less than 10% of the activity with myo-inositol hexakisphosphate Klebsiella oxytoca glycerol + phosphate - ? 73732 3.1.3.8 glycerol 2-phosphate + H2O 50.8% of the activity with myo-inositol hexakisphosphate Aspergillus niger glycerol + phosphate - ? 73732 3.1.3.8 glycerol 2-phosphate + H2O 85.6% of the activity with myo-inositol hexakisphosphate Rhizopus microsporus var. oligosporus glycerol + phosphate - ? 73732 3.1.3.8 glycerol 2-phosphate + H2O 141% of the activity with myo-inositol hexakisphosphate Volvariella volvacea glycerol + phosphate - ? 73732 3.1.3.8 glycerol 2-phosphate + H2O 98% of the activity with myo-inositol hexakisphosphate Lentinula edodes glycerol + phosphate - ? 73732 3.1.3.8 glycerol 2-phosphate + H2O less than 10% of the activity with myo-inositol hexakisphosphate Klebsiella oxytoca MO-3 glycerol + phosphate - ? 73732 3.1.3.8 glycerol 3-phosphate + H2O - Aspergillus niger glycerol + phosphate - ? 73733 3.1.3.8 glycerol 3-phosphate + H2O 43% of the activity with 4-nitrophenylphosphate Arabidopsis thaliana glycerol + phosphate - ? 73733 3.1.3.8 GTP + H2O - Lupinus albus ? - ? 64639 3.1.3.8 GTP + H2O - Raoultella terrigena ? - ? 64639 3.1.3.8 GTP + H2O 271% relative activity compared to myo-inositol hexakisphosphate Nicotiana tabacum GDP + phosphate - ? 73741 3.1.3.8 inositol 1-monophosphate + H2O 58% activity compared to myo-inositol hexakisphosphate Debaryomyces castellii inositol + phosphate - ? 392261 3.1.3.8 inositol 2-monophosphate + H2O 84% activity compared to myo-inositol hexakisphosphate Debaryomyces castellii inositol + phosphate - ? 392262 3.1.3.8 inositol bisphosphate + H2O - Klebsiella sp. ? - ? 83137 3.1.3.8 inositol bisphosphate + H2O - Klebsiella sp. PG-2 ? - ? 83137 3.1.3.8 inositol monophosphate + H2O - Klebsiella sp. inositol + phosphate - ? 83136 3.1.3.8 inositol monophosphate + H2O - Klebsiella sp. PG-2 inositol + phosphate - ? 83136 3.1.3.8 inositol pentaphosphate + H2O - Klebsiella sp. ? - ? 83140 3.1.3.8 inositol tetraphosphate + H2O - Klebsiella sp. ? - ? 83139 3.1.3.8 inositol tetraphosphate + H2O almost the same activity as with myo-inositol hexakisphosphate Klebsiella oxytoca ? - ? 83139 3.1.3.8 inositol triphosphate + H2O - Klebsiella sp. ? - ? 83138 3.1.3.8 inositol triphosphate + H2O 40% of the activity with myo-inositol hexakisphosphate Klebsiella oxytoca ? - ? 83138 3.1.3.8 L-tyrosine phosphate + H2O 142% relative activity compared to myo-inositol hexakisphosphate Nicotiana tabacum L-tyrosine + phosphate - ? 392441 3.1.3.8 additional information - Aspergillus ficuum ? - ? 89 3.1.3.8 additional information enzyme exhibits phytase and acid phosphatase activity Aspergillus niger ? - ? 89 3.1.3.8 additional information less than 5% of the activity with myo-inositol hexakisphosphate with: ATP, ADP, glycerophosphate, glucose 1-phosphate, glucose 6-phosphate, fructose 6-phosphate and mannose 6-phosphate Citrobacter braakii ? - ? 89 3.1.3.8 additional information no activity with 4-nitrophenyl phosphate, beta-glycerophosphate, glucose 6-phosphate, sodium glycerophosphate, AMP, ADP and ATP Bacillus sp. (in: Bacteria) ? - ? 89 3.1.3.8 additional information no activity with GTP and diphosphate Klebsiella sp. ? - ? 89 3.1.3.8 additional information inducible enzyme Klebsiella sp. ? - ? 89 3.1.3.8 additional information the enzyme production is strongly repressed by inorganic phosphates and requires a high carbon to phosphorus ratio in the medium Aspergillus niger ? - ? 89 3.1.3.8 additional information high levels of phosphorous in the growth medium inhibit phytase synthesis, effect is larger in semisolid medium than in liquid medium Aspergillus ficuum ? - ? 89 3.1.3.8 additional information the enzyme is inducible under carbon limitation in the presence of myo-inositol hexakisphosphate Raoultella terrigena ? - ? 89 3.1.3.8 additional information role of phytase in Ca2+ mobilization during germination of mung bean seed via a salvage pathway that involves allosteric activation by myo-inositol triphosphate Vigna radiata ? - ? 89 3.1.3.8 additional information degradation of phytate by high-phytase Saccharomyces cerevisiae strains during simulated gastrointestinal digestion. The enzyme may be a 3-phytase, EC 3.1.3.8, or a 6-phytase, EC 3.1.3.26. The product of the hydrolysis of myo-inositol hexakisphosphate i.e. myo-inositol 1,2,3,4,5-pentakisphosphate or myo-inositol 1,3,4,5,6-pentakisphosphate has not been identified Saccharomyces cerevisiae ? - ? 89 3.1.3.8 additional information phytases from bifidobacteria could be partly active in human gut and could contribute to phytic acid degradation during food processing Bifidobacterium pseudocatenulatum ? - ? 89 3.1.3.8 additional information phytases from bifidobacteria partly could be active in human gut and could contribute to phytic acid degradation during food processing Bifidobacterium adolescentis ? - ? 89 3.1.3.8 additional information phytases from bifidobacteria partly could be active in human gut and could contribute to phytic acid degradation during food processing Bifidobacterium pseudolongum subsp. globosum ? - ? 89 3.1.3.8 additional information phytases from bifidobacteria partly could be active in human gut and could contribute to phytic acid degradation during food processing Bifidobacterium longum ? - ? 89 3.1.3.8 additional information phytases from bifidobacteria partly could be active in human gut and could contribute to phytic acid degradation during food processing Bifidobacterium angulatum ? - ? 89 3.1.3.8 additional information no activity towards 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate and 1-L-myo-inositol 1,2,5,6-tetrakisphosphate Pantoea agglomerans ? - ? 89 3.1.3.8 additional information to reduce phytates in dried grains with solubles (DDGS) and corn gluten feed (CGF), a phytase from Aspergillus niger, PhyA, is investigated regarding its capability to catalyze the hydrolysis of phytates in light steep water and whole stillage. Dephosphorylation of phytates in light steep water and whole stillage proceeds via the formation of InsP5, InsP4, InsP3, and InsP2 intermediates with phosphate and InsP1 as the end products. During the process, the amount of phosphate in the substrates is increased from 54% to 66% in the whole stillage, and from 20% to 90% in the light steep water, suggesting to a substantial dephosphorylation of the phytates in the light steep water and whole stillage via PhyA catalyzed hydrolysis. The most effective period of degradation is during the first 2 h for whole stillage and 6 h for light steep water Aspergillus niger ? - ? 89 3.1.3.8 additional information substrate specificity of recombinant isozymes, overview Triticum aestivum ? - ? 89 3.1.3.8 additional information substrate specificity of recombinant isozymes, overview Hordeum vulgare ? - ? 89 3.1.3.8 additional information no substrate: 4-nitrophenyl phosphate, catalytic efficiency is about 0.04 per mM and s Aspergillus niger ? - ? 89 3.1.3.8 additional information no substrate: 4-nitrophenyl phosphate, catalytic efficiency is about 0.1 per mM and s Aspergillus japonicus ? - ? 89 3.1.3.8 additional information phytase hydrolyzes and liberates inorganic phosphate from Ca2+, Mg2+, and Co2+ phytates more efficiently than those of Al3+, Fe2+, Fe3+, and Zn2+ Thermothelomyces heterothallicus ? - ? 89 3.1.3.8 additional information substrate specificities of wild-type and mutant enzymes, overview Bacillus sp. (in: Bacteria) ? - ? 89 3.1.3.8 additional information HcBPP preferentially recognizes its substrate and selectively hydrolyzes insoluble Ca2+-phytate salts at three phosphate group sites, yielding the final product, myo-inositol 2,4,6-trisphosphate, exhibiting also the 1- and 5-phytase activities. Ins(2,4,6)P3 is unable to bind Ca2+ or any other cation tested, including Co2+, Cu2+, Fe3+, Mg2+, Mn2+, Sr2+, and Zn2+ Hahella chejuensis ? - ? 89 3.1.3.8 additional information phytase ASr1 acts primarily as a scavenger of phosphate groups locked in the phytic acid molecule Klebsiella sp. ? - ? 89 3.1.3.8 additional information the cell-bound phytase is able to effectively dephytinize wheat flour, wheat bran, rice flour, and soybean flour, or soymilk, to varied extents Wickerhamomyces anomalus ? - ? 89 3.1.3.8 additional information phytase activity is determined using the ferrous sulfate-molybdenum blue method Serratia sp. ? - ? 89 3.1.3.8 additional information substrate binding structure modelling, all six phosphate groups of phytate are involved in a hydrogen bond network connecting the substrate with PhyK, induced conformational changes upon substrate binding, overview Klebsiella sp. ? - ? 89 3.1.3.8 additional information substrate specificity of the incomplete domain and the functional relationship of tandemly repeated domains in beta-propeller phytases. The incomplete domain Phy-DI is not functional with myo-inositol-1,2,3,4,5,6-hexakisphosphate at 35°C and pH 7.0 Bacillus sp. (in: Bacteria) ? - ? 89 3.1.3.8 additional information substrate specificity of the recombinant isozyme, overview Zea mays ? - ? 89 3.1.3.8 additional information substrate specificity with substrates Na-phytate, i.e. myo-inositol-1,2,3,4,5,6-hexakisphosphate, naphtyl-1 phosphate, 4-nitrophenylphosphate, ATP, and ADP, overview Aspergillus niger ? - ? 89 3.1.3.8 additional information the enzyme catalyzes the dephytinization of soymilk Wickerhamomyces anomalus ? - ? 89 3.1.3.8 additional information the enzyme exhibits a broad substrate selectivity, substrate are e.g. ATP, ADP, AMP, glucose-6-phosphate, fructose-6-phosphate, 4-nitrophenyl phosphate, and beta-glycerophosphate Aspergillus ficuum ? - ? 89 3.1.3.8 additional information the native enzyme is also active on ADP, ATP, alpha- and beta-glycerophosphate, 2-naphthyl phosphate, and 4-nitrophenyl phosphate. No activity with sodium tripolyphosphate, fructose-6-phosphate, diphosphate, AMP Bacillus subtilis ? - ? 89 3.1.3.8 additional information the phytase exhibits broad substrate specificity since it hydrolyses 4-nitrophenyl phosphate, ATP, ADP, and glucose-6-phosphate besides phytic acid. The enzyme hydrolyzes insoluble calcium and magnesium phytates efficiently, but not iron phytate Wickerhamomyces anomalus ? - ? 89 3.1.3.8 additional information the phytase isozymes exhibit a broad affinity for various phosphorylated compounds, i.e. 4-nitrophenyl phosphate 1-naphthyl phosphate, 2-naphthyl phosphate, AMP, ATP, GTP, fructose-1, 6-bisphosphate, fructose-6-phosphate, glucose-6-phosphate, and pyridoxal phosphate, substrate specificities, overview Rhizopus microsporus var. oligosporus ? - ? 89 3.1.3.8 additional information the substrate specificity of LlALP from lily pollen is unique among phytases, it differs from that of acid phytases, which show broad substrate specificity, and from other alkaline phytases, which exhibit narrow substrate specificity including lack of activity against 4-nitrophenyl phosphate. LlALP from lily pollen hydrolyzes phytate and pNPP Lilium longiflorum ? - ? 89 3.1.3.8 additional information the substrate specificity of LlALP from lily pollen is unique among phytases, it differs from that of acid phytases, which show broad substrate specificity, and from other alkaline phytases, which exhibit narrow substrate specificity including lack of activity against 4-nitrophenyl phosphate. LlALP from lily pollen hydrolyzes phytate and pNPP. The recombinant LlALP2 expressed in Pichia pastoris hydrolyzes phytate and 4-nitrophenyl phosphate and exhibits no activity towards ATP. Activity against 4-nitrophenyl phosphate is nearly 2.5fold higher than against phytate, similar to what is observed with the native enzyme Lilium longiflorum ? - ? 89 3.1.3.8 additional information enzyme is a 6-phytase, EC 3.1.3.26. Major degradation product is DL-inositol 1,2,3,4,5-pentaphosphate Hafnia alvei ? - ? 89 3.1.3.8 additional information enzyme is a 6-phytase, EC 3.1.3.26. Major degradation product is DL-inositol 1,2,3,4,5-pentaphosphate Yersinia kristensenii ? - ? 89 3.1.3.8 additional information high substrate specificity for sodium phytate. No substrates: 4-nitrophenyl phosphate, D-glucose 1-phosphate, ATP, D-fructose-1,6-diphosphate, glycerol 2-phosphate Niallia nealsonii ? - ? 89 3.1.3.8 additional information determination of phytase activity of bacteriocins producing lactic acid bacteria previously isolated from spontaneous rye sourdough. The results show that the highest extracellular phytase activity produces Pediococcus pentosaceus KTU05-8 and KTU05-9 strains, as compared to Lactobacillus sakei strain KTU05-6, Pediococcus acidilactici strain KTU05-7, and Pediococcus pentosaceus KTU05-10, with a volumetric phytase activity of 32 and 54 U/ml, respectively, under conditions similar to leavening of bread dough (pH 5.5 and 30°C). In vitro studies in simulated gastrointestinal tract media pH provide that bioproducts prepared with Pediococcus pentosaceus strains used in wholemeal wheat bread preparation increase solubility of iron, zinc, manganese, calcium, and phosphorus average 30% Pediococcus pentosaceus ? - ? 89 3.1.3.8 additional information effect of sourdough on the myo-inositol phosphates levels, overview Bifidobacterium pseudocatenulatum ? - ? 89 3.1.3.8 additional information effect of sourdough on the myo-inositol phosphates levels, overview Bifidobacterium longum subsp. infantis ? - ? 89 3.1.3.8 additional information the bifunctional enzyme also exhibits glucose-1-phosphatase, cf. EC 3.1.3.10 Pantoea sp. 3.5.1 ? - ? 89 3.1.3.8 additional information ammonium vanadate and ammonium molybdate staining method for activity determination. The recombinant enzyme produces myo-inositol pentakisphosphate, myo-inositol tetrakisphosphate, and myo-inositol triphosphate from myo-inositol hexakisphosphate, respectively Bifidobacterium pseudocatenulatum ? - ? 89 3.1.3.8 additional information ammonium vanadate and ammonium molybdate staining method for activity determination. The recombinant enzyme produces myo-inositol pentakisphosphate, myo-inositol tetrakisphosphate, and myo-inositol triphosphate from myo-inositol hexakisphosphate, respectively Bifidobacterium longum subsp. infantis ? - ? 89 3.1.3.8 additional information among the many phosphate conjugate substrates PhyA shows fairly high specificity for phytate Penicillium oxalicum ? - ? 89 3.1.3.8 additional information color reagents, containing 33.3% v/v nitric acid, 10% w/v ammonium molybdate, and 0.24% w/v ammonium vanadate v/v in a 2:1:1 ratio, for stop of enzyme reaction and product determination Rhodotorula mucilaginosa ? - ? 89 3.1.3.8 additional information enzyme activity assay using color reagent containing 1.5% w/v ammonium molybdate, 5.5% v/v sulfuric acid solution, and 2.7% w/v ferrous sulfate Bacillus subtilis ? - ? 89 3.1.3.8 additional information enzyme PhyA efficiently releases phosphate from feedstuffs such as soybean, rich bran, and corn meal. The enzyme shows significant substrate specificity for phytate and only low activity with 4-nitrophenyl phosphate, diphosphate, 2-naphthyl phosphate, alpha-glyceryl phosphate, beta-glycerylphosphate, glucose 1-phosphate, fructose 1-phosphate, fructose 6-phosphate, AMP, ADP, and ATP Penicillium oxalicum ? - ? 89 3.1.3.8 additional information enzyme rPhysXT52 shows nonspecific phosphohydrolytic activity with 4-nitrophenyl phosphate, 1-naphthyl phosphate, 2-naphthyl phosphate, phenyl phosphate, 2-glycerophosphate, glucose-6-phosphate, fructose-6-phosphate, fructose-1,6-diphosphate, AMP, ADP, ATP, and NADP+, overview Dendroctonus frontalis ? - ? 89 3.1.3.8 additional information enzyme rSt-Phy also shows haloperoxidase activity with KBr, metavanadate, and H2O2, only histidine acid phosphatases with the active site sequence RHGXRXP can function as haloperoxidase, overview Thermothelomyces heterothallicus ? - ? 89 3.1.3.8 additional information glucose-1-phosphatase enzymes and, specifically, Agp phytases are known to have a broad substrate specificity. The bifunctional enzyme from Pantoea sp. 3.5.1 exhibits 3-phytase activity, and also glucose-1-phosphatase, cf. EC 3.1.3.10. Broad substrate specificity, substrates are phytate and many other phosphate-containing substrates, such as AMP, NADP, pNPP, 1-naphthyl phosphate, glucose phosphates, beta-glycerol phosphate, and diphosphate, overview Pantoea sp. 3.5.1 ? - ? 89 3.1.3.8 additional information hydrolysis of insoluble metal-phytates, overview. The enzyme is also active with 4-nitrophenyl phosphate, sodium diphosphate, glucose-1-phosphate, and glucose-6-phosphate Enterobacter sp. ACSS ? - ? 89 3.1.3.8 additional information lower activity with 4-nitrophenyl phosphate, no or poor activity with AMP, ADP, ATP, GTP, NADP+, glucose 1-phosphate, and glucose 6-phosphate uncultured bacterium ? - ? 89 3.1.3.8 additional information no considerable activity with other phosphorylated substrates like ATP, ADP, dSPP, pNPP, glucose 6-phosphate, and fructose 6-phosphate Priestia aryabhattai ? - ? 89 3.1.3.8 additional information the C-terminal domain is catalytically active, while the N-terminal domain exhibits no measurable myo-inositol hexakisphosphate phosphohydrolase activity Pseudomonas sp. ? - ? 89 3.1.3.8 additional information the enzyme also performs unspecific phosphomonoesterase (PME) activity with substrate 4-nitrophenyl phosphate. Phytase and PME activities in Evernia prunastri strongly co-vary among sites of sample collection with contrasting N deposition Evernia prunastri ? - ? 89 3.1.3.8 additional information the enzyme also shows moderate activity with 4-nitrophenyl phosphate, beta-glycerylphosphate, glucose 1-phosphate, fructose 1,6-bisphosphate, and glucose 6-phosphate, and low activity with AMP, ADP, and ATP. The enzyme cleaves phytate in soybean meal, rapeseed meal, and corn meal Morchella importuna ? - ? 89 3.1.3.8 additional information the enzyme also shows phosphate-monoester phosphohydrolase activity, EC 3.1.3.2 Hordeum vulgare ? - ? 89 3.1.3.8 additional information the enzyme catalyzes phosphate release from cereals such as corn, soybean, and wheat Penicillium chrysogenum ? - ? 89 3.1.3.8 additional information the enzyme is also able to dephosphorylate ATP and ADP with 95 and 29% of the activity compared to phytic acid, respectively Bacillus amyloliquefaciens ? - ? 89 3.1.3.8 additional information the enzyme is also active with glucose 6-phosphate, but not with NADP+, ATP, and diphosphate Loigolactobacillus coryniformis ? - ? 89 3.1.3.8 additional information the enzyme shows a broad substrate specificity with highest activity for calcium phytate, the enzyme is also active with ADP, ATP, glycerol beta-phosphate, glucose 6-phosphate, and 4-nitrophenyl phosphate. Among all the feed samples, mustard oil cake is dephytinized more efficiently than otherfeed samples, e.g. cotton oil cake Aspergillus oryzae ? - ? 89 3.1.3.8 additional information the enzyme shows broad substrate specificity, but the highest activity is observed with phytic acid. Other substrates are 4-nitrophenyl phosphate, ATP, glucose-6-phosphate, and glycerol-3-phosphate. Phosphate detection by ammonium heptamolybdate reagent Burkholderia sp. a13(2014) ? - ? 89 3.1.3.8 additional information the enzyme shows no or poor activity with ATP, ADP, 4-nitrophenyl phosphate, diphosphate, glucose 6-phosphate, and fructose 6-phosphate Shigella sp. CD2 ? - ? 89 3.1.3.8 additional information the ferrous sulfate-molybdenum blue method is used for enzyme activity detection Yersinia enterocolitica ? - ? 89 3.1.3.8 additional information the ferrous sulfate-molybdenum blue method is used for enzyme activity detection Yersinia kristensenii ? - ? 89 3.1.3.8 additional information the phytase demonstrates high substrate specificity for sodium phytate. The enzyme also hydrolyzes 4-nitrophenyl phosphate, ATP, AMPc, glucose 6-phosphate, glucose 1-phosphate, and UDPG Rhizopus microsporus ? - ? 89 3.1.3.8 additional information the purified enzyme shows specificity to different salts of phytic acid, it also shows phosphomonoesterase activity with ADP and ATP (high activity), while not with 4-nitrophenyl phosphate and glucose 6-phosphate Bacillus subtilis subsp. subtilis ? - ? 89 3.1.3.8 additional information the recombinant enzyme displayed broad substrate specificity. Molecular modeling and docking of phytase with various substrates show differential binding patterns, overview. Strong binding affinity with ATP and phytic acid, while the lowest with AMP and phosphoenol pyruvate. The enzyme is also active with ATP and 4-nitrophenyl phosphate Thermothelomyces heterothallicus ? - ? 89 3.1.3.8 additional information usage of chromogenic agent containing 1:3 mixture of 100 g/l ammonium molybdate, and 0.785 g/l ammonium metavanadate in 15% HNO3 for the phosphate product detection in enzyme assay Bacillus amyloliquefaciens ? - ? 89 3.1.3.8 additional information vanadate exhibits competitive inhibition of phytase, making it bifunctional to act as haloperoxidase Wickerhamomyces anomalus ? - ? 89 3.1.3.8 additional information phytases from bifidobacteria partly could be active in human gut and could contribute to phytic acid degradation during food processing Bifidobacterium adolescentis ATCC 15703 ? - ? 89 3.1.3.8 additional information no substrate: 4-nitrophenyl phosphate, catalytic efficiency is about 0.1 per mM and s Aspergillus japonicus BCC18313 ? - ? 89 3.1.3.8 additional information among the many phosphate conjugate substrates PhyA shows fairly high specificity for phytate Penicillium oxalicum PJ3 ? - ? 89 3.1.3.8 additional information the enzyme is also able to dephosphorylate ATP and ADP with 95 and 29% of the activity compared to phytic acid, respectively Bacillus amyloliquefaciens US573 ? - ? 89 3.1.3.8 additional information the native enzyme is also active on ADP, ATP, alpha- and beta-glycerophosphate, 2-naphthyl phosphate, and 4-nitrophenyl phosphate. No activity with sodium tripolyphosphate, fructose-6-phosphate, diphosphate, AMP Bacillus subtilis CF92 ? - ? 89 3.1.3.8 additional information the enzyme production is strongly repressed by inorganic phosphates and requires a high carbon to phosphorus ratio in the medium Aspergillus niger NRRL 4361 ? - ? 89 3.1.3.8 additional information the enzyme production is strongly repressed by inorganic phosphates and requires a high carbon to phosphorus ratio in the medium Aspergillus niger NRRL 326 ? - ? 89 3.1.3.8 additional information high substrate specificity for sodium phytate. No substrates: 4-nitrophenyl phosphate, D-glucose 1-phosphate, ATP, D-fructose-1,6-diphosphate, glycerol 2-phosphate Niallia nealsonii ZJ0702 ? - ? 89 3.1.3.8 additional information effect of sourdough on the myo-inositol phosphates levels, overview Bifidobacterium longum subsp. infantis S12 ? - ? 89 3.1.3.8 additional information ammonium vanadate and ammonium molybdate staining method for activity determination. The recombinant enzyme produces myo-inositol pentakisphosphate, myo-inositol tetrakisphosphate, and myo-inositol triphosphate from myo-inositol hexakisphosphate, respectively Bifidobacterium longum subsp. infantis S12 ? - ? 89 3.1.3.8 additional information - Aspergillus ficuum WB 4541 ? - ? 89 3.1.3.8 additional information the enzyme exhibits a broad substrate selectivity, substrate are e.g. ATP, ADP, AMP, glucose-6-phosphate, fructose-6-phosphate, 4-nitrophenyl phosphate, and beta-glycerophosphate Aspergillus ficuum NTG-23 ? - ? 89 3.1.3.8 additional information usage of chromogenic agent containing 1:3 mixture of 100 g/l ammonium molybdate, and 0.785 g/l ammonium metavanadate in 15% HNO3 for the phosphate product detection in enzyme assay Bacillus amyloliquefaciens DSM 1061 ? - ? 89 3.1.3.8 additional information substrate specificity of the incomplete domain and the functional relationship of tandemly repeated domains in beta-propeller phytases. The incomplete domain Phy-DI is not functional with myo-inositol-1,2,3,4,5,6-hexakisphosphate at 35°C and pH 7.0 Bacillus sp. (in: Bacteria) HJB17 ? - ? 89 3.1.3.8 additional information color reagents, containing 33.3% v/v nitric acid, 10% w/v ammonium molybdate, and 0.24% w/v ammonium vanadate v/v in a 2:1:1 ratio, for stop of enzyme reaction and product determination Rhodotorula mucilaginosa JMUY14 ? - ? 89 3.1.3.8 additional information the enzyme shows broad substrate specificity, but the highest activity is observed with phytic acid. Other substrates are 4-nitrophenyl phosphate, ATP, glucose-6-phosphate, and glycerol-3-phosphate. Phosphate detection by ammonium heptamolybdate reagent Burkholderia sp. a13(2014) JCM 30421 ? - ? 89 3.1.3.8 additional information the enzyme catalyzes phosphate release from cereals such as corn, soybean, and wheat Penicillium chrysogenum CCT 1273 ? - ? 89 3.1.3.8 additional information no considerable activity with other phosphorylated substrates like ATP, ADP, dSPP, pNPP, glucose 6-phosphate, and fructose 6-phosphate Priestia aryabhattai RS1 ? - ? 89 3.1.3.8 additional information degradation of phytate by high-phytase Saccharomyces cerevisiae strains during simulated gastrointestinal digestion. The enzyme may be a 3-phytase, EC 3.1.3.8, or a 6-phytase, EC 3.1.3.26. The product of the hydrolysis of myo-inositol hexakisphosphate i.e. myo-inositol 1,2,3,4,5-pentakisphosphate or myo-inositol 1,3,4,5,6-pentakisphosphate has not been identified Saccharomyces cerevisiae BY85 ? - ? 89 3.1.3.8 additional information the C-terminal domain is catalytically active, while the N-terminal domain exhibits no measurable myo-inositol hexakisphosphate phosphohydrolase activity Pseudomonas sp. FB15 ? - ? 89 3.1.3.8 additional information the enzyme is also active with glucose 6-phosphate, but not with NADP+, ATP, and diphosphate Loigolactobacillus coryniformis MH121153 ? - ? 89 3.1.3.8 additional information enzyme PhyA efficiently releases phosphate from feedstuffs such as soybean, rich bran, and corn meal. The enzyme shows significant substrate specificity for phytate and only low activity with 4-nitrophenyl phosphate, diphosphate, 2-naphthyl phosphate, alpha-glyceryl phosphate, beta-glycerylphosphate, glucose 1-phosphate, fructose 1-phosphate, fructose 6-phosphate, AMP, ADP, and ATP Penicillium oxalicum KCTC6440 ? - ? 89 3.1.3.8 additional information no activity with 4-nitrophenyl phosphate, beta-glycerophosphate, glucose 6-phosphate, sodium glycerophosphate, AMP, ADP and ATP Bacillus sp. (in: Bacteria) KHU-10 ? - ? 89 3.1.3.8 additional information effect of sourdough on the myo-inositol phosphates levels, overview Bifidobacterium longum subsp. infantis JCM 1222 ? - ? 89 3.1.3.8 additional information ammonium vanadate and ammonium molybdate staining method for activity determination. The recombinant enzyme produces myo-inositol pentakisphosphate, myo-inositol tetrakisphosphate, and myo-inositol triphosphate from myo-inositol hexakisphosphate, respectively Bifidobacterium longum subsp. infantis JCM 1222 ? - ? 89 3.1.3.8 additional information - Aspergillus ficuum WB 4016 ? - ? 89 3.1.3.8 additional information effect of sourdough on the myo-inositol phosphates levels, overview Bifidobacterium longum subsp. infantis NCTC 11817 ? - ? 89 3.1.3.8 additional information ammonium vanadate and ammonium molybdate staining method for activity determination. The recombinant enzyme produces myo-inositol pentakisphosphate, myo-inositol tetrakisphosphate, and myo-inositol triphosphate from myo-inositol hexakisphosphate, respectively Bifidobacterium longum subsp. infantis NCTC 11817 ? - ? 89 3.1.3.8 additional information effect of sourdough on the myo-inositol phosphates levels, overview Bifidobacterium longum subsp. infantis DSM 20088 ? - ? 89 3.1.3.8 additional information ammonium vanadate and ammonium molybdate staining method for activity determination. The recombinant enzyme produces myo-inositol pentakisphosphate, myo-inositol tetrakisphosphate, and myo-inositol triphosphate from myo-inositol hexakisphosphate, respectively Bifidobacterium longum subsp. infantis DSM 20088 ? - ? 89 3.1.3.8 additional information the enzyme production is strongly repressed by inorganic phosphates and requires a high carbon to phosphorus ratio in the medium Aspergillus niger NRRL 330 ? - ? 89 3.1.3.8 additional information phytase activity is determined using the ferrous sulfate-molybdenum blue method Serratia sp. TN49, ACCC 03909 ? - ? 89 3.1.3.8 additional information substrate specificity of recombinant isozymes, overview Triticum aestivum Bob white SH 98 26 ? - ? 89 3.1.3.8 additional information effect of sourdough on the myo-inositol phosphates levels, overview Bifidobacterium longum subsp. infantis ATCC 15697 ? - ? 89 3.1.3.8 additional information ammonium vanadate and ammonium molybdate staining method for activity determination. The recombinant enzyme produces myo-inositol pentakisphosphate, myo-inositol tetrakisphosphate, and myo-inositol triphosphate from myo-inositol hexakisphosphate, respectively Bifidobacterium longum subsp. infantis ATCC 15697 ? - ? 89 3.1.3.8 additional information - Aspergillus ficuum X ? - ? 89 3.1.3.8 additional information - Aspergillus ficuum K ? - ? 89 3.1.3.8 additional information the purified enzyme shows specificity to different salts of phytic acid, it also shows phosphomonoesterase activity with ADP and ATP (high activity), while not with 4-nitrophenyl phosphate and glucose 6-phosphate Bacillus subtilis subsp. subtilis JJBS250 ? - ? 89 3.1.3.8 additional information substrate specificities of wild-type and mutant enzymes, overview Bacillus sp. (in: Bacteria) MD2 ? - ? 89 3.1.3.8 additional information no activity with GTP and diphosphate Klebsiella sp. ASR1 ? - ? 89 3.1.3.8 additional information the enzyme production is strongly repressed by inorganic phosphates and requires a high carbon to phosphorus ratio in the medium Aspergillus niger NRRL 372 ? - ? 89 3.1.3.8 additional information - Aspergillus ficuum WB 320 ? - ? 89 3.1.3.8 additional information the enzyme shows a broad substrate specificity with highest activity for calcium phytate, the enzyme is also active with ADP, ATP, glycerol beta-phosphate, glucose 6-phosphate, and 4-nitrophenyl phosphate. Among all the feed samples, mustard oil cake is dephytinized more efficiently than otherfeed samples, e.g. cotton oil cake Aspergillus oryzae SBS50 ? - ? 89 3.1.3.8 additional information less than 5% of the activity with myo-inositol hexakisphosphate with: ATP, ADP, glycerophosphate, glucose 1-phosphate, glucose 6-phosphate, fructose 6-phosphate and mannose 6-phosphate Citrobacter braakii YH-15 ? - ? 89 3.1.3.8 additional information substrate specificity with substrates Na-phytate, i.e. myo-inositol-1,2,3,4,5,6-hexakisphosphate, naphtyl-1 phosphate, 4-nitrophenylphosphate, ATP, and ADP, overview Aspergillus niger FS3 ? - ? 89 3.1.3.8 additional information - Aspergillus ficuum WB 364 ? - ? 89 3.1.3.8 additional information inducible enzyme Klebsiella sp. PG-2 ? - ? 89 3.1.3.8 additional information the enzyme also shows moderate activity with 4-nitrophenyl phosphate, beta-glycerylphosphate, glucose 1-phosphate, fructose 1,6-bisphosphate, and glucose 6-phosphate, and low activity with AMP, ADP, and ATP. The enzyme cleaves phytate in soybean meal, rapeseed meal, and corn meal Morchella importuna SCYDJ1A1 ? - ? 89 3.1.3.8 additional information the enzyme production is strongly repressed by inorganic phosphates and requires a high carbon to phosphorus ratio in the medium Aspergillus niger NRRL337 ? - ? 89 3.1.3.8 additional information - Aspergillus ficuum NRRL 3135 ? - ? 89 3.1.3.8 additional information the enzyme production is strongly repressed by inorganic phosphates and requires a high carbon to phosphorus ratio in the medium Aspergillus niger NRRL372 ? - ? 89 3.1.3.8 additional information - Aspergillus ficuum WB4781 ? - ? 89 3.1.3.8 additional information enzyme activity assay using color reagent containing 1.5% w/v ammonium molybdate, 5.5% v/v sulfuric acid solution, and 2.7% w/v ferrous sulfate Bacillus subtilis DR8886 ? - ? 89 3.1.3.8 additional information no substrate: 4-nitrophenyl phosphate, catalytic efficiency is about 0.04 per mM and s Aspergillus niger BCC18081 ? - ? 89 3.1.3.8 additional information effect of sourdough on the myo-inositol phosphates levels, overview Bifidobacterium pseudocatenulatum ATCC 27919 ? - ? 89 3.1.3.8 additional information ammonium vanadate and ammonium molybdate staining method for activity determination. The recombinant enzyme produces myo-inositol pentakisphosphate, myo-inositol tetrakisphosphate, and myo-inositol triphosphate from myo-inositol hexakisphosphate, respectively Bifidobacterium pseudocatenulatum ATCC 27919 ? - ? 89 3.1.3.8 myo-inositol 1,2,4,5,6-pentakisphosphate + H2O - Bacillus amyloliquefaciens myo-inositol-2,4,5,6 tetrakisphosphate + phosphate - ? 392675 3.1.3.8 myo-inositol 1,2,4,5,6-pentakisphosphate + H2O - Bacillus amyloliquefaciens DS11 myo-inositol-2,4,5,6 tetrakisphosphate + phosphate - ? 392675 3.1.3.8 myo-inositol 2,4,5,6-tetrakisphosphate + H2O - Bacillus amyloliquefaciens myo-inositol 2,4,6-trisphosphate + phosphate - ? 392676 3.1.3.8 myo-inositol 2,4,5,6-tetrakisphosphate + H2O - Bacillus amyloliquefaciens DS11 myo-inositol 2,4,6-trisphosphate + phosphate - ? 392676 3.1.3.8 myo-inositol hexakisphosphate + 3 H2O enzyme efficiently hydrolyzes Ca2+-phytate salts and yields myo-inositol 2,4,6-trisphosphate and three phosphate groups as final products Pseudomonas sp. myo-inositol 2,4,6-trisphosphate + 3 phosphate - ? 430424 3.1.3.8 myo-inositol hexakisphosphate + 3 H2O enzyme efficiently hydrolyzes Ca2+-phytate salts and yields myo-inositol 2,4,6-trisphosphate and three phosphate groups as final products Pseudomonas sp. BS10-3 myo-inositol 2,4,6-trisphosphate + 3 phosphate - ? 430424 3.1.3.8 myo-inositol hexakisphosphate + H2O - Aspergillus fumigatus myo-inositol pentakisphosphate + phosphate - ? 73702 3.1.3.8 myo-inositol hexakisphosphate + H2O - Aspergillus ficuum myo-inositol pentakisphosphate + phosphate - ? 73702 3.1.3.8 myo-inositol hexakisphosphate + H2O - Bacillus subtilis ? + phosphate - ? 73704 3.1.3.8 myo-inositol hexakisphosphate + H2O - Escherichia coli ? + phosphate - ? 73704 3.1.3.8 myo-inositol hexakisphosphate + H2O - Aspergillus niger ? + phosphate - ? 73704 3.1.3.8 myo-inositol hexakisphosphate + H2O - Glycine max ? + phosphate - ? 73704 3.1.3.8 myo-inositol hexakisphosphate + H2O - Klebsiella aerogenes ? + phosphate - ? 73704 3.1.3.8 myo-inositol hexakisphosphate + H2O - Pseudomonas sp. ? + phosphate - ? 73704 3.1.3.8 myo-inositol hexakisphosphate + H2O - Pantoea agglomerans ? + phosphate - ? 73704 3.1.3.8 myo-inositol hexakisphosphate + H2O - Agaricus bisporus ? + phosphate - ? 73704 3.1.3.8 myo-inositol hexakisphosphate + H2O - Rhizopus arrhizus ? + phosphate - ? 73704 3.1.3.8 myo-inositol hexakisphosphate + H2O - Bacillus sp. (in: Bacteria) ? + phosphate - ? 73704 3.1.3.8 myo-inositol hexakisphosphate + H2O - Bacillus amyloliquefaciens ? + phosphate - ? 73704 3.1.3.8 myo-inositol hexakisphosphate + H2O - Selenomonas ruminantium ? + phosphate - ? 73704 3.1.3.8 myo-inositol hexakisphosphate + H2O - Candida tropicalis ? + phosphate - ? 73704 3.1.3.8 myo-inositol hexakisphosphate + H2O - Neurospora sp. ? + phosphate - ? 73704 3.1.3.8 myo-inositol hexakisphosphate + H2O - Aspergillus amstelodami ? + phosphate - ? 73704 3.1.3.8 myo-inositol hexakisphosphate + H2O - Aspergillus flavus ? + phosphate - ? 73704 3.1.3.8 myo-inositol hexakisphosphate + H2O - Schwanniomyces occidentalis ? + phosphate - ? 73704 3.1.3.8 myo-inositol hexakisphosphate + H2O - Enterobacter sp. ? + phosphate - ? 73704 3.1.3.8 myo-inositol hexakisphosphate + H2O - Aspergillus candidus ? + phosphate - ? 73704 3.1.3.8 myo-inositol hexakisphosphate + H2O - Penicillium sp. ? + phosphate - ? 73704 3.1.3.8 myo-inositol hexakisphosphate + H2O - Wickerhamomyces anomalus ? + phosphate - ? 73704 3.1.3.8 myo-inositol hexakisphosphate + H2O - Klebsiella sp. ? + phosphate - ? 73704 3.1.3.8 myo-inositol hexakisphosphate + H2O - Penicillium simplicissimum ? + phosphate - ? 73704 3.1.3.8 myo-inositol hexakisphosphate + H2O - Aspergillus fumigatus ? + phosphate - ? 73704 3.1.3.8 myo-inositol hexakisphosphate + H2O - Metschnikowia pulcherrima ? + phosphate - ? 73704 3.1.3.8 myo-inositol hexakisphosphate + H2O - Thermomyces lanuginosus ? + phosphate - ? 73704 3.1.3.8 myo-inositol hexakisphosphate + H2O - Kluyveromyces lactis ? + phosphate - ? 73704 3.1.3.8 myo-inositol hexakisphosphate + H2O - Botrytis cinerea ? + phosphate - ? 73704 3.1.3.8 myo-inositol hexakisphosphate + H2O - Aspergillus carbonarius ? + phosphate - ? 73704 3.1.3.8 myo-inositol hexakisphosphate + H2O - Aspergillus ficuum ? + phosphate - ? 73704 3.1.3.8 myo-inositol hexakisphosphate + H2O - Thermothelomyces heterothallicus ? + phosphate - ? 73704 3.1.3.8 myo-inositol hexakisphosphate + H2O - Klebsiella oxytoca ? + phosphate - ? 73704 3.1.3.8 myo-inositol hexakisphosphate + H2O - Aspergillus versicolor ? + phosphate - ? 73704 3.1.3.8 myo-inositol hexakisphosphate + H2O - Aspergillus carneus ? + phosphate - ? 73704 3.1.3.8 myo-inositol hexakisphosphate + H2O - Rhizopus microsporus var. oligosporus ? + phosphate - ? 73704 3.1.3.8 myo-inositol hexakisphosphate + H2O - Schwanniomyces castellii ? + phosphate - ? 73704 3.1.3.8 myo-inositol hexakisphosphate + H2O - Rhizopus stolonifer ? + phosphate - ? 73704 3.1.3.8 myo-inositol hexakisphosphate + H2O - Blastobotrys adeninivorans ? + phosphate - ? 73704 3.1.3.8 myo-inositol hexakisphosphate + H2O - Geotrichum candidum ? + phosphate - ? 73704 3.1.3.8 myo-inositol hexakisphosphate + H2O - Aspergillus pseudoglaucus ? + phosphate - ? 73704 3.1.3.8 myo-inositol hexakisphosphate + H2O - [Candida] intermedia ? + phosphate - ? 73704 3.1.3.8 myo-inositol hexakisphosphate + H2O - Mitsuokella multacida ? + phosphate - ? 73704 3.1.3.8 myo-inositol hexakisphosphate + H2O - Aspergillus wentii ? + phosphate - ? 73704 3.1.3.8 myo-inositol hexakisphosphate + H2O - Torulaspora delbrueckii ? + phosphate - ? 73704 3.1.3.8 myo-inositol hexakisphosphate + H2O - Mucor racemosus ? + phosphate - ? 73704 3.1.3.8 myo-inositol hexakisphosphate + H2O - Torulaspora globosa ? + phosphate - ? 73704 3.1.3.8 myo-inositol hexakisphosphate + H2O - Citrobacter braakii ? + phosphate - ? 73704 3.1.3.8 myo-inositol hexakisphosphate + H2O - Lactobacillus amylovorus ? + phosphate - ? 73704 3.1.3.8 myo-inositol hexakisphosphate + H2O - Aspergillus terreus ? + phosphate - ? 73704 3.1.3.8 myo-inositol hexakisphosphate + H2O - Penicillium caseoicolum ? + phosphate - ? 73704 3.1.3.8 myo-inositol hexakisphosphate + H2O - Clavispora lusitaniae ? + phosphate - ? 73704 3.1.3.8 myo-inositol hexakisphosphate + H2O - Hanseniaspora valbyensis ? + phosphate - ? 73704 3.1.3.8 myo-inositol hexakisphosphate + H2O - Lachancea thermotolerans ? + phosphate - ? 73704 3.1.3.8 myo-inositol hexakisphosphate + H2O - Cyberlindnera rhodanensis ? + phosphate - ? 73704 3.1.3.8 myo-inositol hexakisphosphate + H2O - Scheffersomyces spartinae ? + phosphate - ? 73704 3.1.3.8 myo-inositol hexakisphosphate + H2O - Aspergillus chevalieri ? + phosphate - ? 73704 3.1.3.8 myo-inositol hexakisphosphate + H2O - Aspergillus syndowi ? + phosphate - ? 73704 3.1.3.8 myo-inositol hexakisphosphate + H2O - Mucor piriformis ? + phosphate - ? 73704 3.1.3.8 myo-inositol hexakisphosphate + H2O - Thermomyces dupontii ? + phosphate - ? 73704 3.1.3.8 myo-inositol hexakisphosphate + H2O - Schwanniomyces yamadae ? + phosphate - ? 73704 3.1.3.8 myo-inositol hexakisphosphate + H2O - Torulaspora pretoriensis ? + phosphate - ? 73704 3.1.3.8 myo-inositol hexakisphosphate + H2O - Lachancea kluyveri ? + phosphate - ? 73704 3.1.3.8 myo-inositol hexakisphosphate + H2O - Aspergillus nidulans ? + phosphate - ? 73704 3.1.3.8 myo-inositol hexakisphosphate + H2O - Bacillus licheniformis ? + phosphate - ? 73704 3.1.3.8 myo-inositol hexakisphosphate + H2O - Obesumbacterium proteus ? + phosphate - ? 73704 3.1.3.8 myo-inositol hexakisphosphate + H2O constitutive Schwanniomyces castellii ? + phosphate - ? 73704 3.1.3.8 myo-inositol hexakisphosphate + H2O hydrolyzes phytate in a stepwise manner Lupinus albus ? + phosphate - ? 73704 3.1.3.8 myo-inositol hexakisphosphate + H2O product release is the rate limiting step of the reaction Aspergillus niger ? + phosphate - ? 73704 3.1.3.8 myo-inositol hexakisphosphate + H2O rapid equilibrium ordered mechanism in which binding of Ca2+ to the active site is necessary for the essential activation of the enzyme. Ca2+ turns out to be also required for the substrate because the enzyme is only able to hydrolyze the calcium-phythate complex Bacillus amyloliquefaciens ? + phosphate - ? 73704 3.1.3.8 myo-inositol hexakisphosphate + H2O step-wise hydrolysis of myo-inositol hexakisphosphate Corylus avellana ? + phosphate - ? 73704 3.1.3.8 myo-inositol hexakisphosphate + H2O the enzyme is able to hydrolyze any of the six phosphate groups of phytate. The reaction is likely to proceed through a direct attack of the metal-bridging water molecule on the phosphorous atom of a substrate and the subsequent stabilization of the pentavalent transition state by the bound calcium ions Bacillus subtilis ? + phosphate - ? 73704 3.1.3.8 myo-inositol hexakisphosphate + H2O very specific for phytate Pseudomonas syringae ? + phosphate - ? 73704 3.1.3.8 myo-inositol hexakisphosphate + H2O very specific for, no activity on other phosphate esters Bacillus sp. (in: Bacteria) ? + phosphate - ? 73704 3.1.3.8 myo-inositol hexakisphosphate + H2O inducible enzyme Bacillus subtilis ? + phosphate - ? 73704 3.1.3.8 myo-inositol hexakisphosphate + H2O inducible enzyme Enterobacter sp. ? + phosphate - ? 73704 3.1.3.8 myo-inositol hexakisphosphate + H2O inducible enzyme Lactobacillus amylovorus ? + phosphate - ? 73704 3.1.3.8 myo-inositol hexakisphosphate + H2O constitutive enzyme Escherichia coli ? + phosphate - ? 73704 3.1.3.8 myo-inositol hexakisphosphate + H2O constitutive enzyme Klebsiella aerogenes ? + phosphate - ? 73704 3.1.3.8 myo-inositol hexakisphosphate + H2O constitutive enzyme Pseudomonas sp. ? + phosphate - ? 73704 3.1.3.8 myo-inositol hexakisphosphate + H2O constitutive enzyme Bacillus sp. (in: Bacteria) ? + phosphate - ? 73704 3.1.3.8 myo-inositol hexakisphosphate + H2O constitutive enzyme Selenomonas ruminantium ? + phosphate - ? 73704 3.1.3.8 myo-inositol hexakisphosphate + H2O constitutive enzyme Aspergillus carbonarius ? + phosphate - ? 73704 3.1.3.8 myo-inositol hexakisphosphate + H2O constitutive enzyme Aspergillus carneus ? + phosphate - ? 73704 3.1.3.8 myo-inositol hexakisphosphate + H2O constitutive enzyme Rhizopus microsporus var. oligosporus ? + phosphate - ? 73704 3.1.3.8 myo-inositol hexakisphosphate + H2O constitutive enzyme Blastobotrys adeninivorans ? + phosphate - ? 73704 3.1.3.8 myo-inositol hexakisphosphate + H2O constitutive enzyme Cyberlindnera rhodanensis ? + phosphate - ? 73704 3.1.3.8 myo-inositol hexakisphosphate + H2O constitutive enzyme Scheffersomyces spartinae ? + phosphate - ? 73704 3.1.3.8 myo-inositol hexakisphosphate + H2O constitutive enzyme Aspergillus awamori ? + phosphate - ? 73704 3.1.3.8 myo-inositol hexakisphosphate + H2O inducible Klebsiella aerogenes ? + phosphate - ? 73704 3.1.3.8 myo-inositol hexakisphosphate + H2O the enzyme may be a 3-phytase, EC 3.1.3.8, or a 6-phytase, EC 3.1.3.26. The product of the hydrolysis of myo-inositol hexakisphosphate (i.e. myo-inositol 1,2,3,4,5-pentakisphosphate) or myo-inositol 1,3,4,5,6-pentakisphosphate has not been identified Saccharomyces cerevisiae ? + phosphate - ? 73704 3.1.3.8 myo-inositol hexakisphosphate + H2O the enzyme may be a 3-phytase, EC 3.1.3.8, or a 6-phytase, EC 3.1.3.26. The product of the hydrolysis of myo-inositol hexakisphosphate i.e. myo-inositol 1,2,3,4,5-pentakisphosphate or myo-inositol 1,3,4,5,6-pentakisphosphate has not been identified Aspergillus niger ? + phosphate - ? 73704 3.1.3.8 myo-inositol hexakisphosphate + H2O The enzyme may be a 3-phytase, EC 3.1.3.8, or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate (3-phytase) or 1D-myo-inositol 1,2,3,5,6-pentakisphosphate (4-phytase) (i.e. 1L-myo-inositol 1,2,3,4,5-pentakisphosphate if 1L numbering is applied) has not been analyzed. The reaction was monitored by analyzing the released phosphate Aspergillus niger ? + phosphate - ? 73704 3.1.3.8 myo-inositol hexakisphosphate + H2O - Bacillus sp. (in: Bacteria) DS1 ? + phosphate - ? 73704 3.1.3.8 myo-inositol hexakisphosphate + H2O constitutive enzyme Bacillus sp. (in: Bacteria) DS1 ? + phosphate - ? 73704 3.1.3.8 myo-inositol hexakisphosphate + H2O product release is the rate limiting step of the reaction Aspergillus niger NRRL 3135 ? + phosphate - ? 73704 3.1.3.8 myo-inositol hexakisphosphate + H2O the enzyme may be a 3-phytase, EC 3.1.3.8, or a 6-phytase, EC 3.1.3.26. The product of the hydrolysis of myo-inositol hexakisphosphate (i.e. myo-inositol 1,2,3,4,5-pentakisphosphate) or myo-inositol 1,3,4,5,6-pentakisphosphate has not been identified Saccharomyces cerevisiae BY85 ? + phosphate - ? 73704 3.1.3.8 myo-inositol hexakisphosphate + H2O very specific for, no activity on other phosphate esters Bacillus sp. (in: Bacteria) KHU-10 ? + phosphate - ? 73704 3.1.3.8 myo-inositol hexakisphosphate + H2O very specific for phytate Pseudomonas syringae MOK1 ? + phosphate - ? 73704 3.1.3.8 myo-inositol hexakisphosphate + H2O - Klebsiella sp. PG-2 ? + phosphate - ? 73704 3.1.3.8 myo-inositol hexakisphosphate + H2O - Aspergillus terreus CBS ? + phosphate - ? 73704 3.1.3.8 myo-inositol hexakisphosphate + H2O product release is the rate limiting step of the reaction Aspergillus niger T213 ? + phosphate - ? 73704 3.1.3.8 myo-inositol hexakisphosphate + H2O rapid equilibrium ordered mechanism in which binding of Ca2+ to the active site is necessary for the essential activation of the enzyme. Ca2+ turns out to be also required for the substrate because the enzyme is only able to hydrolyze the calcium-phythate complex Bacillus amyloliquefaciens S11 ? + phosphate - ? 73704 3.1.3.8 myo-inositol hexakisphosphate + H2O - Bacillus subtilis 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate - ? 83134 3.1.3.8 myo-inositol hexakisphosphate + H2O - Escherichia coli 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate - ? 83134 3.1.3.8 myo-inositol hexakisphosphate + H2O - Saccharomyces cerevisiae 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate - ? 83134 3.1.3.8 myo-inositol hexakisphosphate + H2O - Aspergillus niger 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate - ? 83134 3.1.3.8 myo-inositol hexakisphosphate + H2O - Klebsiella aerogenes 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate - ? 83134 3.1.3.8 myo-inositol hexakisphosphate + H2O - Pseudomonas sp. 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate - ? 83134 3.1.3.8 myo-inositol hexakisphosphate + H2O - Ogataea angusta 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate - ? 83134 3.1.3.8 myo-inositol hexakisphosphate + H2O - Pantoea agglomerans 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate - ? 83134 3.1.3.8 myo-inositol hexakisphosphate + H2O - Bacillus amyloliquefaciens 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate - ? 83134 3.1.3.8 myo-inositol hexakisphosphate + H2O - Selenomonas ruminantium 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate - ? 83134 3.1.3.8 myo-inositol hexakisphosphate + H2O - Candida tropicalis 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate - ? 83134 3.1.3.8 myo-inositol hexakisphosphate + H2O - Kluyveromyces marxianus 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate - ? 83134 3.1.3.8 myo-inositol hexakisphosphate + H2O - Torulopsis candida 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate - ? 83134 3.1.3.8 myo-inositol hexakisphosphate + H2O - Citrobacter freundii 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate - ? 83134 3.1.3.8 myo-inositol hexakisphosphate + H2O - Neurospora sp. 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate - ? 83134 3.1.3.8 myo-inositol hexakisphosphate + H2O - Aspergillus flavus 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate - ? 83134 3.1.3.8 myo-inositol hexakisphosphate + H2O - Aspergillus oryzae 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate - ? 83134 3.1.3.8 myo-inositol hexakisphosphate + H2O - Aspergillus terreus 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate - ? 83134 3.1.3.8 myo-inositol hexakisphosphate + H2O - Aspergillus phoenicis 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate - ? 83134 3.1.3.8 myo-inositol hexakisphosphate + H2O - Raoultella terrigena 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate - ? 83134 3.1.3.8 myo-inositol hexakisphosphate + H2O - Saccharomyces sp. 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate - ? 83134 3.1.3.8 myo-inositol hexakisphosphate + H2O - Aspergillus sp. 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate - ? 83134 3.1.3.8 myo-inositol hexakisphosphate + H2O - Penicillium sp. 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate - ? 83134 3.1.3.8 myo-inositol hexakisphosphate + H2O - Klebsiella sp. 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate - ? 83134 3.1.3.8 myo-inositol hexakisphosphate + H2O - Aspergillus awamori 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate - ? 83134 3.1.3.8 myo-inositol hexakisphosphate + H2O - Aspergillus fumigatus 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate - ? 83134 3.1.3.8 myo-inositol hexakisphosphate + H2O - Penicillium camemberti 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate - ? 83134 3.1.3.8 myo-inositol hexakisphosphate + H2O - Macrophomina phaseolina 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate - ? 83134 3.1.3.8 myo-inositol hexakisphosphate + H2O - Aspergillus carbonarius 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate - ? 83134 3.1.3.8 myo-inositol hexakisphosphate + H2O - Aspergillus ficuum 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate - ? 83134 3.1.3.8 myo-inositol hexakisphosphate + H2O - Thermothelomyces heterothallicus 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate - ? 83134 3.1.3.8 myo-inositol hexakisphosphate + H2O - Klebsiella oxytoca 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate - ? 83134 3.1.3.8 myo-inositol hexakisphosphate + H2O - Aspergillus tubingensis 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate - ? 83134 3.1.3.8 myo-inositol hexakisphosphate + H2O - Aspergillus versicolor 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate - ? 83134 3.1.3.8 myo-inositol hexakisphosphate + H2O - Aspergillus flavipes 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate - ? 83134 3.1.3.8 myo-inositol hexakisphosphate + H2O - Mucor sp. 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate - ? 83134 3.1.3.8 myo-inositol hexakisphosphate + H2O - Aspergillus carneus 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate - ? 83134 3.1.3.8 myo-inositol hexakisphosphate + H2O - Rhizopus microsporus var. oligosporus 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate - ? 83134 3.1.3.8 myo-inositol hexakisphosphate + H2O - Debaryomyces castellii 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate - ? 83134 3.1.3.8 myo-inositol hexakisphosphate + H2O - Schwanniomyces castellii 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate - ? 83134 3.1.3.8 myo-inositol hexakisphosphate + H2O - Paramecium sp. 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate - ? 83134 3.1.3.8 myo-inositol hexakisphosphate + H2O - Millerozyma farinosa 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate - ? 83134 3.1.3.8 myo-inositol hexakisphosphate + H2O - Thermomyces dupontii 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate - ? 83134 3.1.3.8 myo-inositol hexakisphosphate + H2O - Aspergillus nidulans 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate - ? 83134 3.1.3.8 myo-inositol hexakisphosphate + H2O - Kodamaea ohmeri 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate - ? 83134 3.1.3.8 myo-inositol hexakisphosphate + H2O - Aspergillus ficuum 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate a mixture of two pentaphosphates: the major component is 1-myo-inositol 1,2,4,5,6-pentakisphosphate and the other is 1-myo-inositol 1,2,3,4,5-pentakisphosphate ? 83134 3.1.3.8 myo-inositol hexakisphosphate + H2O about 85% of myo-inositol hexakisphosphate in soybean meal is hydrolyzed by the enzyme whereas only 67% of the myo-inositol hexakisphosphate in cottonseed meal is hydrolyzed by the same enzyme treatment Aspergillus ficuum 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate - ? 83134 3.1.3.8 myo-inositol hexakisphosphate + H2O 100% relative activity Nicotiana tabacum 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate - ? 83134 3.1.3.8 myo-inositol hexakisphosphate + H2O 100% activity Debaryomyces castellii 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate - ? 83134 3.1.3.8 myo-inositol hexakisphosphate + H2O preferred substrate, 100% activity Papiliotrema laurentii 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate - ? 83134 3.1.3.8 myo-inositol hexakisphosphate + H2O calcium myo-inositol hexakisphosphate Aspergillus niger 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate - ? 83134 3.1.3.8 myo-inositol hexakisphosphate + H2O dodecasodium myo-inositol hexakisphosphate Aspergillus niger 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate - ? 83134 3.1.3.8 myo-inositol hexakisphosphate + H2O - Aspergillus niger NRRL 4361 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate - ? 83134 3.1.3.8 myo-inositol hexakisphosphate + H2O - Aspergillus niger NRRL 326 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate - ? 83134 3.1.3.8 myo-inositol hexakisphosphate + H2O - Aspergillus niger 92 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate - ? 83134 3.1.3.8 myo-inositol hexakisphosphate + H2O - Selenomonas ruminantium JY35 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate - ? 83134 3.1.3.8 myo-inositol hexakisphosphate + H2O - Thermothelomyces heterothallicus BJTLR50 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate - ? 83134 3.1.3.8 myo-inositol hexakisphosphate + H2O - Aspergillus fumigatus WY-2 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate - ? 83134 3.1.3.8 myo-inositol hexakisphosphate + H2O - Debaryomyces castellii CBS 2923 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate - ? 83134 3.1.3.8 myo-inositol hexakisphosphate + H2O preferred substrate, 100% activity Papiliotrema laurentii ABO 510 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate - ? 83134 3.1.3.8 myo-inositol hexakisphosphate + H2O - Aspergillus ficuum WB 4541 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate - ? 83134 3.1.3.8 myo-inositol hexakisphosphate + H2O - Aspergillus niger Naturphos 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate - ? 83134 3.1.3.8 myo-inositol hexakisphosphate + H2O - Saccharomyces cerevisiae CY 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate - ? 83134 3.1.3.8 myo-inositol hexakisphosphate + H2O - Aspergillus niger N-J 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate - ? 83134 3.1.3.8 myo-inositol hexakisphosphate + H2O - Aspergillus niger NRRL 3135 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate - ? 83134 3.1.3.8 myo-inositol hexakisphosphate + H2O - Bacillus subtilis VTT E-68013 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate - ? 83134 3.1.3.8 myo-inositol hexakisphosphate + H2O - Aspergillus niger IIIAn/8 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate - ? 83134 3.1.3.8 myo-inositol hexakisphosphate + H2O - Aspergillus terreus 9A1 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate - ? 83134 3.1.3.8 myo-inositol hexakisphosphate + H2O - Aspergillus tubingensis NRRL 4875 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate - ? 83134 3.1.3.8 myo-inositol hexakisphosphate + H2O - Aspergillus ficuum WB 4016 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate - ? 83134 3.1.3.8 myo-inositol hexakisphosphate + H2O - Aspergillus niger NRRL 330 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate - ? 83134 3.1.3.8 myo-inositol hexakisphosphate + H2O - Aspergillus ficuum X 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate - ? 83134 3.1.3.8 myo-inositol hexakisphosphate + H2O - Aspergillus ficuum K 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate - ? 83134 3.1.3.8 myo-inositol hexakisphosphate + H2O - Klebsiella sp. ASR1 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate - ? 83134 3.1.3.8 myo-inositol hexakisphosphate + H2O - Aspergillus niger NRRL 372 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate - ? 83134 3.1.3.8 myo-inositol hexakisphosphate + H2O - Aspergillus ficuum WB 320 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate - ? 83134 3.1.3.8 myo-inositol hexakisphosphate + H2O - Rhizopus microsporus var. oligosporus NRRL 2710 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate - ? 83134 3.1.3.8 myo-inositol hexakisphosphate + H2O - Bacillus amyloliquefaciens DSM 7 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate - ? 83134 3.1.3.8 myo-inositol hexakisphosphate + H2O - Aspergillus niger NRRL 65 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate - ? 83134 3.1.3.8 myo-inositol hexakisphosphate + H2O - Kodamaea ohmeri BG3 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate - ? 83134 3.1.3.8 myo-inositol hexakisphosphate + H2O - Aspergillus ficuum WB 364 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate - ? 83134 3.1.3.8 myo-inositol hexakisphosphate + H2O - Aspergillus niger NRRL337 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate - ? 83134 3.1.3.8 myo-inositol hexakisphosphate + H2O - Aspergillus niger CB 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate - ? 83134 3.1.3.8 myo-inositol hexakisphosphate + H2O - Aspergillus ficuum NRRL 3135 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate - ? 83134 3.1.3.8 myo-inositol hexakisphosphate + H2O - Aspergillus terreus CBS 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate - ? 83134 3.1.3.8 myo-inositol hexakisphosphate + H2O - Aspergillus niger NRRL372 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate - ? 83134 3.1.3.8 myo-inositol hexakisphosphate + H2O - Aspergillus ficuum WB4781 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate - ? 83134 3.1.3.8 myo-inositol hexakisphosphate + H2O - Aspergillus niger T213 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate - ? 83134 3.1.3.8 myo-inositol hexakisphosphate + H2O - Klebsiella oxytoca MO-3 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate - ? 83134 3.1.3.8 myo-inositol hexakisphosphate + H2O - Bacillus subtilis D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate after 10 min D-myo-inositol 1,2,4,5,6-pentakisphosphate is the major degradation product, accompanied by small amounts of D-myo-inositol 1,2,3,4,6-pentakisphosphate, D-myo-inositol 2,4,5,6-tetrakisphosphate and D-myo-inositol 1,2,35-tetrakisphosphate. After 30 min, the quantity of D-myo-inositol 1,2,4,5,6-pentakisphosphate decreases and the levels of D-myo-inositol 2,4,5,6-tetrakisphosphate and D-myo-inositol 1,2,35-tetrakisphosphate increases. After 90 min the major products are Ins(1,3,5) P3 and Ins(2,4,6)P3 ? 364597 3.1.3.8 myo-inositol hexakisphosphate + H2O step-wise dephosphorylation occurs via 1. D-myo-inositol 1,2,4,5,6-pentakisphosphate, 2. D-myo-inositol 1,2,5,6-tetrakisphosphate or D-myo-inositol 2,4,5,6-tetrakisphosphate, 3. D-myo-inositol 1,2,6-trisphosphate, D-myo-inositol 1,2,3-trisphosphate or D-myo-inositol 1,4,5-trisphosphate, 4. myo-inositol 1,2-bisphosphate or myo-inositol 2,5-bisphosphate, myo-inositol 4,5-bisphosphate or myo-inositol 2,4-bisphosphate. Myo-inositol 2-monophosphate is the final product Klebsiella sp. D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate - ? 364597 3.1.3.8 myo-inositol hexakisphosphate + H2O - Bacillus subtilis VTT E-68013 D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate after 10 min D-myo-inositol 1,2,4,5,6-pentakisphosphate is the major degradation product, accompanied by small amounts of D-myo-inositol 1,2,3,4,6-pentakisphosphate, D-myo-inositol 2,4,5,6-tetrakisphosphate and D-myo-inositol 1,2,35-tetrakisphosphate. After 30 min, the quantity of D-myo-inositol 1,2,4,5,6-pentakisphosphate decreases and the levels of D-myo-inositol 2,4,5,6-tetrakisphosphate and D-myo-inositol 1,2,35-tetrakisphosphate increases. After 90 min the major products are Ins(1,3,5) P3 and Ins(2,4,6)P3 ? 364597 3.1.3.8 myo-inositol hexakisphosphate + H2O - Escherichia coli myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate - ? 392678 3.1.3.8 myo-inositol hexakisphosphate + H2O - Bacillus amyloliquefaciens myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate - ? 392678 3.1.3.8 myo-inositol hexakisphosphate + H2O - Schizophyllum commune myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate - ? 392678 3.1.3.8 myo-inositol hexakisphosphate + H2O - Volvariella volvacea myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate - ? 392678 3.1.3.8 myo-inositol hexakisphosphate + H2O - Bacillus subtilis myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate - ? 392678 3.1.3.8 myo-inositol hexakisphosphate + H2O - Lentinula edodes myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate - ? 392678 3.1.3.8 myo-inositol hexakisphosphate + H2O - Penicillium oxalicum myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate - ? 392678 3.1.3.8 myo-inositol hexakisphosphate + H2O - Bacillus sp. (in: Bacteria) myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate - ? 392678 3.1.3.8 myo-inositol hexakisphosphate + H2O - Schizophyllum commune LPB 101 myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate - ? 392678 3.1.3.8 myo-inositol hexakisphosphate + H2O - Bacillus sp. (in: Bacteria) DS11 myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate - ? 392678 3.1.3.8 myo-inositol hexakisphosphate + H2O - Bacillus amyloliquefaciens DS11 myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate - ? 392678 3.1.3.8 myo-inositol hexakisphosphate + H2O - Escherichia coli 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + 1D-myo-inositol-1,2,5,6 tetrakisphosphate + phosphate - ? 412465 3.1.3.8 myo-inositol hexakisphosphate + H2O - Aspergillus niger 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + 1D-myo-inositol-1,2,5,6 tetrakisphosphate + phosphate - ? 412465 3.1.3.8 myo-inositol hexakisphosphate + H2O - Pectobacterium carotovorum 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + 1D-myo-inositol-1,2,5,6 tetrakisphosphate + phosphate - ? 412465 3.1.3.8 myo-inositol hexakisphosphate + H2O - Papiliotrema laurentii 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + 1D-myo-inositol-1,2,5,6 tetrakisphosphate + phosphate - ? 412465 3.1.3.8 myo-inositol hexakisphosphate + H2O - Penicillium parvum 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + 1D-myo-inositol-1,2,5,6 tetrakisphosphate + phosphate - ? 412465 3.1.3.8 myo-inositol hexakisphosphate + H2O - Bacillus sp. (in: Bacteria) 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + 1D-myo-inositol-1,2,5,6 tetrakisphosphate + phosphate - ? 412465 3.1.3.8 myo-inositol hexakisphosphate + H2O - Yersinia rohdei 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + 1D-myo-inositol-1,2,5,6 tetrakisphosphate + phosphate - ? 412465 3.1.3.8 myo-inositol hexakisphosphate + H2O - Yersinia pestis 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + 1D-myo-inositol-1,2,5,6 tetrakisphosphate + phosphate - ? 412465 3.1.3.8 myo-inositol hexakisphosphate + H2O - Yersinia kristensenii 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + 1D-myo-inositol-1,2,5,6 tetrakisphosphate + phosphate - ? 412465 3.1.3.8 myo-inositol hexakisphosphate + H2O - Pectobacterium wasabiae 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + 1D-myo-inositol-1,2,5,6 tetrakisphosphate + phosphate - ? 412465 3.1.3.8 myo-inositol hexakisphosphate + H2O - Bacillus subtilis 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + 1D-myo-inositol-1,2,5,6 tetrakisphosphate + phosphate - ? 412465 3.1.3.8 myo-inositol hexakisphosphate + H2O sodium salt Yersinia frederiksenii 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + 1D-myo-inositol-1,2,5,6 tetrakisphosphate + phosphate - ? 412465 3.1.3.8 myo-inositol hexakisphosphate + H2O - Penicillium parvum BCC1769 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + 1D-myo-inositol-1,2,5,6 tetrakisphosphate + phosphate - ? 412465 3.1.3.8 myo-inositol hexakisphosphate + H2O - Bacillus subtilis US417 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + 1D-myo-inositol-1,2,5,6 tetrakisphosphate + phosphate - ? 412465 3.1.3.8 myo-inositol hexakisphosphate + H2O - Aspergillus niger NRRL 3135 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + 1D-myo-inositol-1,2,5,6 tetrakisphosphate + phosphate - ? 412465 3.1.3.8 myo-inositol hexakisphosphate + H2O - Bacillus sp. (in: Bacteria) DECSR1 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + 1D-myo-inositol-1,2,5,6 tetrakisphosphate + phosphate - ? 412465 3.1.3.8 myo-inositol hexakisphosphate + H2O - Papiliotrema laurentii AL27 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + 1D-myo-inositol-1,2,5,6 tetrakisphosphate + phosphate - ? 412465 3.1.3.8 myo-inositol hexakisphosphate + H2O 7% of the activity with 4-nitrophenylphosphate Arabidopsis thaliana myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate - ? 412468 3.1.3.8 myo-inositol hexakisphosphate + H2O sodium salt Aspergillus ficuum myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate - ? 412468 3.1.3.8 myo-inositol hexakisphosphate + H2O - Hafnia alvei DL-inositol 1,2,4,5,6-pentaphosphate + phosphate 10-14% of product ? 430426 3.1.3.8 myo-inositol hexakisphosphate + H2O - Yersinia kristensenii DL-inositol 1,2,4,5,6-pentaphosphate + phosphate 10-14% of product ? 430426 3.1.3.8 myo-inositol hexakisphosphate + H2O - Bacillus subtilis 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate - ? 446313 3.1.3.8 myo-inositol hexakisphosphate + H2O - Evernia prunastri 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate - ? 446313 3.1.3.8 myo-inositol hexakisphosphate + H2O - Thermothelomyces heterothallicus 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate - ? 446313 3.1.3.8 myo-inositol hexakisphosphate + H2O - Rhizopus microsporus 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate - ? 446313 3.1.3.8 myo-inositol hexakisphosphate + H2O - Wickerhamomyces anomalus 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate - ? 446313 3.1.3.8 myo-inositol hexakisphosphate + H2O - Yersinia enterocolitica 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate - ? 446313 3.1.3.8 myo-inositol hexakisphosphate + H2O - Thermothelomyces thermophilus 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate - ? 446313 3.1.3.8 myo-inositol hexakisphosphate + H2O - Pantoea sp. 3.5.1 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate - ? 446313 3.1.3.8 myo-inositol hexakisphosphate + H2O - Dendroctonus frontalis 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate - ? 446313 3.1.3.8 myo-inositol hexakisphosphate + H2O - Aspergillus niger 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate - ? 446313 3.1.3.8 myo-inositol hexakisphosphate + H2O - Aspergillus oryzae 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate - ? 446313 3.1.3.8 myo-inositol hexakisphosphate + H2O - Bacillus amyloliquefaciens 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate - ? 446313 3.1.3.8 myo-inositol hexakisphosphate + H2O - Morchella importuna 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate - ? 446313 3.1.3.8 myo-inositol hexakisphosphate + H2O - Lobaria pulmonaria 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate - ? 446313 3.1.3.8 myo-inositol hexakisphosphate + H2O - Peltigera membranacea 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate - ? 446313 3.1.3.8 myo-inositol hexakisphosphate + H2O - Bacillus subtilis subsp. subtilis 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate - ? 446313 3.1.3.8 myo-inositol hexakisphosphate + H2O - Yersinia mollaretii 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate - ? 446313 3.1.3.8 myo-inositol hexakisphosphate + H2O - Pseudomonas sp. 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate - ? 446313 3.1.3.8 myo-inositol hexakisphosphate + H2O - Streptomyces sp. 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate - ? 446313 3.1.3.8 myo-inositol hexakisphosphate + H2O - Ramalina fraxinea 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate - ? 446313 3.1.3.8 myo-inositol hexakisphosphate + H2O - Bryoria fuscescens 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate - ? 446313 3.1.3.8 myo-inositol hexakisphosphate + H2O - Pseudevernia furfuracea 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate - ? 446313 3.1.3.8 myo-inositol hexakisphosphate + H2O - Ramalina calicaris 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate - ? 446313 3.1.3.8 myo-inositol hexakisphosphate + H2O - Cladonia portentosa 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate - ? 446313 3.1.3.8 myo-inositol hexakisphosphate + H2O - Hypogymnia physodes 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate - ? 446313 3.1.3.8 myo-inositol hexakisphosphate + H2O - Parmelia saxatilis 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate - ? 446313 3.1.3.8 myo-inositol hexakisphosphate + H2O - Platismatia glauca 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate - ? 446313 3.1.3.8 myo-inositol hexakisphosphate + H2O - Ramalina farinacea 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate - ? 446313 3.1.3.8 myo-inositol hexakisphosphate + H2O - Usnea subfloridana 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate - ? 446313 3.1.3.8 myo-inositol hexakisphosphate + H2O - Amycolatopsis vancoresmycina 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate - ? 446313 3.1.3.8 myo-inositol hexakisphosphate + H2O highly preferred substrate Dendroctonus frontalis 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate - ? 446313 3.1.3.8 myo-inositol hexakisphosphate + H2O best substrate is calcium phytate, sodium phytate results in 60% activity compared to calcium phytate Aspergillus oryzae 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate - ? 446313 3.1.3.8 myo-inositol hexakisphosphate + H2O highest activity at 2.05 mM substrate Bacillus subtilis 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate - ? 446313 3.1.3.8 myo-inositol hexakisphosphate + H2O i.e. phytase Thermothelomyces thermophilus 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate - ? 446313 3.1.3.8 myo-inositol hexakisphosphate + H2O i.e. phytate Rhizopus microsporus 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate - ? 446313 3.1.3.8 myo-inositol hexakisphosphate + H2O i.e. phytate Wickerhamomyces anomalus 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate - ? 446313 3.1.3.8 myo-inositol hexakisphosphate + H2O i.e. phytate Bacillus amyloliquefaciens 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate - ? 446313 3.1.3.8 myo-inositol hexakisphosphate + H2O phytate Bacillus subtilis 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate - ? 446313 3.1.3.8 myo-inositol hexakisphosphate + H2O phytate Amycolatopsis vancoresmycina 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate - ? 446313 3.1.3.8 myo-inositol hexakisphosphate + H2O phytic acid Bacillus amyloliquefaciens 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate - ? 446313 3.1.3.8 myo-inositol hexakisphosphate + H2O phytic acid, sodium phytate is preferred compared to calcium phytate and potassium phytate Bacillus subtilis subsp. subtilis 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate - ? 446313 3.1.3.8 myo-inositol hexakisphosphate + H2O substrate is calcium phytate Thermothelomyces heterothallicus 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate - ? 446313 3.1.3.8 myo-inositol hexakisphosphate + H2O substrates are sodium phytate, potassium phytate, and calcium phytate. Highest activity with calcium phytate Thermothelomyces heterothallicus 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate - ? 446313 3.1.3.8 myo-inositol hexakisphosphate + H2O - Yersinia mollaretii CNY 7263 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate - ? 446313 3.1.3.8 myo-inositol hexakisphosphate + H2O - Bacillus amyloliquefaciens US573 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate - ? 446313 3.1.3.8 myo-inositol hexakisphosphate + H2O phytic acid Bacillus amyloliquefaciens US573 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate - ? 446313 3.1.3.8 myo-inositol hexakisphosphate + H2O - Thermothelomyces thermophilus ATCC 42464 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate - ? 446313 3.1.3.8 myo-inositol hexakisphosphate + H2O - Yersinia mollaretii DSM 18520 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate - ? 446313 3.1.3.8 myo-inositol hexakisphosphate + H2O - Bacillus subtilis 168 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate - ? 446313 3.1.3.8 myo-inositol hexakisphosphate + H2O - Bacillus amyloliquefaciens DSM 1061 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate - ? 446313 3.1.3.8 myo-inositol hexakisphosphate + H2O i.e. phytate Bacillus amyloliquefaciens DSM 1061 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate - ? 446313 3.1.3.8 myo-inositol hexakisphosphate + H2O - Yersinia mollaretii CIP 103324 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate - ? 446313 3.1.3.8 myo-inositol hexakisphosphate + H2O - Yersinia mollaretii ATCC 43969 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate - ? 446313 3.1.3.8 myo-inositol hexakisphosphate + H2O - Pseudomonas sp. FB15 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate - ? 446313 3.1.3.8 myo-inositol hexakisphosphate + H2O - Aspergillus niger N25 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate - ? 446313 3.1.3.8 myo-inositol hexakisphosphate + H2O - Bacillus subtilis subsp. subtilis JJBS250 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate - ? 446313 3.1.3.8 myo-inositol hexakisphosphate + H2O phytic acid, sodium phytate is preferred compared to calcium phytate and potassium phytate Bacillus subtilis subsp. subtilis JJBS250 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate - ? 446313 3.1.3.8 myo-inositol hexakisphosphate + H2O - Aspergillus niger NII 08121 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate - ? 446313 3.1.3.8 myo-inositol hexakisphosphate + H2O - Aspergillus oryzae SBS50 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate - ? 446313 3.1.3.8 myo-inositol hexakisphosphate + H2O best substrate is calcium phytate, sodium phytate results in 60% activity compared to calcium phytate Aspergillus oryzae SBS50 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate - ? 446313 3.1.3.8 myo-inositol hexakisphosphate + H2O - Amycolatopsis vancoresmycina S-12 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate - ? 446313 3.1.3.8 myo-inositol hexakisphosphate + H2O phytate Amycolatopsis vancoresmycina S-12 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate - ? 446313 3.1.3.8 myo-inositol hexakisphosphate + H2O - Bacillus subtilis B.S.46 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate - ? 446313 3.1.3.8 myo-inositol hexakisphosphate + H2O highest activity at 2.05 mM substrate Bacillus subtilis B.S.46 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate - ? 446313 3.1.3.8 myo-inositol hexakisphosphate + H2O - Thermothelomyces thermophilus BCRC 31852 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate - ? 446313 3.1.3.8 myo-inositol hexakisphosphate + H2O - Morchella importuna SCYDJ1A1 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate - ? 446313 3.1.3.8 myo-inositol hexakisphosphate + H2O - Thermothelomyces thermophilus DSM 1799 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate - ? 446313 3.1.3.8 myo-inositol hexakisphosphate + H2O - Yersinia mollaretii WAIP 204 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate - ? 446313 3.1.3.8 myo-inositol hexakisphosphate + H2O - Bacillus subtilis DR8886 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate - ? 446313 3.1.3.8 myo-inositol hexakisphosphate + H2O phytate Bacillus subtilis DR8886 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate - ? 446313 3.1.3.8 myo-inositol hexakisphosphate + H2O - Thermothelomyces thermophilus BJTLR50 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate - ? 446313 3.1.3.8 myo-inositol hexakisphosphate + H2O i.e. phytase Thermothelomyces thermophilus BJTLR50 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate - ? 446313 3.1.3.8 myo-inositol hexakisphosphate + H2O - Streptomyces sp. US42 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate - ? 446313 3.1.3.8 myo-inositol hexakisphosphate + H2O phosphate cleavage position is not determined, cf. EC 3.1.3.8 and 3.1.3.26 Penicillium chrysogenum 1D-myo-inositol pentakisphosphate + phosphate - ? 446314 3.1.3.8 myo-inositol hexakisphosphate + H2O phosphate cleavage position is not determined, cf. EC 3.1.3.8 and 3.1.3.26 Pediococcus pentosaceus 1D-myo-inositol pentakisphosphate + phosphate - ? 446314 3.1.3.8 myo-inositol hexakisphosphate + H2O phosphate cleavage position is not determined, cf. EC 3.1.3.8 and 3.1.3.26 Rhodotorula mucilaginosa 1D-myo-inositol pentakisphosphate + phosphate - ? 446314 3.1.3.8 myo-inositol hexakisphosphate + H2O phosphate cleavage position is not determined, cf. EC 3.1.3.8 and 3.1.3.26 uncultured bacterium 1D-myo-inositol pentakisphosphate + phosphate - ? 446314 3.1.3.8 myo-inositol hexakisphosphate + H2O phosphate cleavage position is not determined, cf. EC 3.1.3.8 and 3.1.3.26 Loigolactobacillus coryniformis 1D-myo-inositol pentakisphosphate + phosphate - ? 446314 3.1.3.8 myo-inositol hexakisphosphate + H2O phosphate cleavage position is not determined, cf. EC 3.1.3.8 and 3.1.3.26 Hordeum vulgare 1D-myo-inositol pentakisphosphate + phosphate - ? 446314 3.1.3.8 myo-inositol hexakisphosphate + H2O phosphate cleavage position is not determined, cf. EC 3.1.3.8 and 3.1.3.26 Penicillium oxalicum 1D-myo-inositol pentakisphosphate + phosphate - ? 446314 3.1.3.8 myo-inositol hexakisphosphate + H2O phosphate cleavage position is not determined, cf. EC 3.1.3.8 and 3.1.3.26 Prevotella ruminicola 1D-myo-inositol pentakisphosphate + phosphate - ? 446314 3.1.3.8 myo-inositol hexakisphosphate + H2O phosphate cleavage position is not determined, cf. EC 3.1.3.8 and 3.1.3.26 Yersinia kristensenii 1D-myo-inositol pentakisphosphate + phosphate - ? 446314 3.1.3.8 myo-inositol hexakisphosphate + H2O phosphate cleavage position is not determined, cf. EC 3.1.3.8 and 3.1.3.26 Bifidobacterium pseudocatenulatum 1D-myo-inositol pentakisphosphate + phosphate - ? 446314 3.1.3.8 myo-inositol hexakisphosphate + H2O phosphate cleavage position is not determined, cf. EC 3.1.3.8 and 3.1.3.26 Bifidobacterium longum subsp. infantis 1D-myo-inositol pentakisphosphate + phosphate - ? 446314 3.1.3.8 myo-inositol hexakisphosphate + H2O phosphate cleavage position is not determined, cf. EC 3.1.3.8 and 3.1.3.26 Burkholderia sp. a13(2014) 1D-myo-inositol pentakisphosphate + phosphate - ? 446314 3.1.3.8 myo-inositol hexakisphosphate + H2O phosphate cleavage position is not determined, cf. EC 3.1.3.8 and 3.1.3.26 Enterobacter sp. ACSS 1D-myo-inositol pentakisphosphate + phosphate - ? 446314 3.1.3.8 myo-inositol hexakisphosphate + H2O phosphate cleavage position is not determined, cf. EC 3.1.3.8 and 3.1.3.26 Priestia aryabhattai 1D-myo-inositol pentakisphosphate + phosphate - ? 446314 3.1.3.8 myo-inositol hexakisphosphate + H2O phosphate cleavage position is not determined, cf. EC 3.1.3.8, 3.1.3.26, and 3.1.3.72 Shigella sp. CD2 1D-myo-inositol pentakisphosphate + phosphate - ? 446314 3.1.3.8 myo-inositol hexakisphosphate + H2O absolutely specific for the substrate, the phosphate cleavage position is not determined, cf. EC 3.1.3.8 and 3.1.3.26 Priestia aryabhattai 1D-myo-inositol pentakisphosphate + phosphate - ? 446314 3.1.3.8 myo-inositol hexakisphosphate + H2O phosphate cleavage position is not determined, cf. EC 3.1.3.8 and 3.1.3.26, preferred substrate is sodium phytate Loigolactobacillus coryniformis 1D-myo-inositol pentakisphosphate + phosphate - ? 446314 3.1.3.8 myo-inositol hexakisphosphate + H2O phosphate cleavage position is not determined, cf. EC 3.1.3.8 and 3.1.3.26, substrate is sodium phytate Penicillium oxalicum 1D-myo-inositol pentakisphosphate + phosphate - ? 446314 3.1.3.8 myo-inositol hexakisphosphate + H2O substrate is sodium phytate or calcium phytate, phosphate cleavage position is not determined, cf. EC 3.1.3.8 and 3.1.3.26 Rhodotorula mucilaginosa 1D-myo-inositol pentakisphosphate + phosphate - ? 446314 3.1.3.8 myo-inositol hexakisphosphate + H2O phosphate cleavage position is not determined, cf. EC 3.1.3.8 and 3.1.3.26 Penicillium oxalicum PJ3 1D-myo-inositol pentakisphosphate + phosphate - ? 446314 3.1.3.8 myo-inositol hexakisphosphate + H2O phosphate cleavage position is not determined, cf. EC 3.1.3.8 and 3.1.3.26 Rhodotorula mucilaginosa JMUY14 1D-myo-inositol pentakisphosphate + phosphate - ? 446314 3.1.3.8 myo-inositol hexakisphosphate + H2O phosphate cleavage position is not determined, cf. EC 3.1.3.8 and 3.1.3.26 Burkholderia sp. a13(2014) JCM 30421 1D-myo-inositol pentakisphosphate + phosphate - ? 446314 3.1.3.8 myo-inositol hexakisphosphate + H2O phosphate cleavage position is not determined, cf. EC 3.1.3.8 and 3.1.3.26 Penicillium chrysogenum CCT 1273 1D-myo-inositol pentakisphosphate + phosphate - ? 446314 3.1.3.8 myo-inositol hexakisphosphate + H2O phosphate cleavage position is not determined, cf. EC 3.1.3.8 and 3.1.3.26 Priestia aryabhattai RS1 1D-myo-inositol pentakisphosphate + phosphate - ? 446314 3.1.3.8 myo-inositol hexakisphosphate + H2O phosphate cleavage position is not determined, cf. EC 3.1.3.8 and 3.1.3.26 Loigolactobacillus coryniformis MH121153 1D-myo-inositol pentakisphosphate + phosphate - ? 446314 3.1.3.8 myo-inositol hexakisphosphate + H2O phosphate cleavage position is not determined, cf. EC 3.1.3.8 and 3.1.3.26 Penicillium oxalicum KCTC6440 1D-myo-inositol pentakisphosphate + phosphate - ? 446314 3.1.3.8 myo-inositol hexakisphosphate disodium salt + H2O - Aspergillus japonicus 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + 1D-myo-inositol-1,2,5,6 tetrakisphosphate + sodium phosphate - ? 412469 3.1.3.8 myo-inositol hexakisphosphate disodium salt + H2O - Aspergillus niger 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + 1D-myo-inositol-1,2,5,6 tetrakisphosphate + sodium phosphate - ? 412469 3.1.3.8 myo-inositol hexakisphosphate disodium salt + H2O - Aspergillus japonicus BCC18313 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + 1D-myo-inositol-1,2,5,6 tetrakisphosphate + sodium phosphate - ? 412469 3.1.3.8 myo-inositol hexakisphosphate disodium salt + H2O - Aspergillus niger BCC18081 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + 1D-myo-inositol-1,2,5,6 tetrakisphosphate + sodium phosphate - ? 412469 3.1.3.8 myo-inositol hexakisphosphate sodium salt + H2O - Aspergillus japonicus 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + 1D-myo-inositol-1,2,5,6 tetrakisphosphate + sodium phosphate - ? 412470 3.1.3.8 myo-inositol hexakisphosphate sodium salt + H2O - Aspergillus niger 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + 1D-myo-inositol-1,2,5,6 tetrakisphosphate + sodium phosphate - ? 412470 3.1.3.8 myo-inositol hexakisphosphate sodium salt + H2O - Aspergillus japonicus BCC18313 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + 1D-myo-inositol-1,2,5,6 tetrakisphosphate + sodium phosphate - ? 412470 3.1.3.8 myo-inositol hexakisphosphate sodium salt + H2O - Aspergillus niger BCC18081 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + 1D-myo-inositol-1,2,5,6 tetrakisphosphate + sodium phosphate - ? 412470 3.1.3.8 myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O - Bacillus subtilis ? + phosphate - ? 415685 3.1.3.8 myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O - Aspergillus niger ? + phosphate - ? 415685 3.1.3.8 myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O - Bacillus sp. (in: Bacteria) ? + phosphate - ? 415685 3.1.3.8 myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O - Rhizopus microsporus var. oligosporus ? + phosphate - ? 415685 3.1.3.8 myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O - Hahella chejuensis ? + phosphate - ? 415685 3.1.3.8 myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O - Wickerhamomyces anomalus ? + phosphate - ? 415685 3.1.3.8 myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O - Yersinia enterocolitica ? + phosphate - ? 415685 3.1.3.8 myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O - Triticum aestivum ? + phosphate - ? 415685 3.1.3.8 myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O - Hordeum vulgare ? + phosphate - ? 415685 3.1.3.8 myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O - Zea mays ? + phosphate - ? 415685 3.1.3.8 myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O cleavage initiation in the dephosphorylation of myo-inositol hexakisphosphate at C3 Aspergillus niger ? + phosphate - ? 415685 3.1.3.8 myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O substrate Na-phytate Bacillus subtilis ? + phosphate - ? 415685 3.1.3.8 myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O - Bacillus subtilis CF92 ? + phosphate - ? 415685 3.1.3.8 myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O substrate Na-phytate Bacillus subtilis CF92 ? + phosphate - ? 415685 3.1.3.8 myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O - Bacillus sp. (in: Bacteria) HJB17 ? + phosphate - ? 415685 3.1.3.8 myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O - Aspergillus niger NRRL 3135 ? + phosphate - ? 415685 3.1.3.8 myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O - Aspergillus niger 113 ? + phosphate - ? 415685 3.1.3.8 myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O - Triticum aestivum Bob white SH 98 26 ? + phosphate - ? 415685 3.1.3.8 myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O although the PhyK homolog AppA is biochemically characterized as a 6-phytase, a co-crystal structure shows the phytate 3-phosphate as scissile group in a similar position to in the active site of PhyK Klebsiella sp. myo-inositol-1,2,4,5,6-pentakisphosphate + phosphate - ? 415686 3.1.3.8 myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O although the PhyK homolog AppA is biochemically characterized as a 6-phytase, a co-crystal structure shows the phytate 3-phosphate as scissile group in a similar position to in the active site of PhyK Klebsiella sp. 1D-myo-inositol-1,2,4,5,6-pentakisphosphate + phosphate - ? 417322 3.1.3.8 NADP+ + H2O 58.1% of the activity with myo-inositol hexakisphosphate, recombinant enzyme Klebsiella sp. NAD+ + phosphate - ? 73730 3.1.3.8 NADP+ + H2O - Raoultella terrigena ? - ? 83160 3.1.3.8 O-phospho-L-serine + H2O - Lupinus albus L-serine + phosphate - ? 364553 3.1.3.8 O-phospho-L-serine + H2O 25% of the activity with 4-nitrophenylphosphate Arabidopsis thaliana L-serine + phosphate - ? 364553 3.1.3.8 O-phospho-L-tyrosine + H2O 28% of the activity with 4-nitrophenylphosphate Arabidopsis thaliana L-tyrosine + phosphate - ? 165625 3.1.3.8 p-nitrophenyl phosphate + H2O - Pantoea agglomerans p-nitrophenol + phosphate - ? 64623 3.1.3.8 p-nitrophenyl phosphate + H2O - Aspergillus niger p-nitrophenol + phosphate - ? 64623 3.1.3.8 p-nitrophenyl phosphate + H2O 135% activity compared to myo-inositol hexakisphosphate Debaryomyces castellii p-nitrophenol + phosphate - ? 64623 3.1.3.8 p-nitrophenyl phosphate + H2O 152.95% activity compared to myo-inositol hexakisphosphate Papiliotrema laurentii p-nitrophenol + phosphate - ? 64623 3.1.3.8 p-nitrophenyl phosphate + H2O 311% relative activity compared to myo-inositol hexakisphosphate Nicotiana tabacum p-nitrophenol + phosphate - ? 64623 3.1.3.8 p-nitrophenyl phosphate + H2O the enzyme shows 22.5% activity towards p-nitrophenyl phosphate compared to myo-inositol hexakisphosphate Bacillus amyloliquefaciens p-nitrophenol + phosphate - ? 64623 3.1.3.8 p-nitrophenyl phosphate + H2O the enzyme shows 22.5% activity towards p-nitrophenyl phosphate compared to myo-inositol hexakisphosphate Klebsiella sp. p-nitrophenol + phosphate - ? 64623 3.1.3.8 p-nitrophenyl phosphate + H2O the enzyme shows 22.5% activity towards p-nitrophenyl phosphate compared to myo-inositol hexakisphosphate Aspergillus ficuum p-nitrophenol + phosphate - ? 64623 3.1.3.8 p-nitrophenyl phosphate + H2O 135% activity compared to myo-inositol hexakisphosphate Debaryomyces castellii CBS 2923 p-nitrophenol + phosphate - ? 64623 3.1.3.8 p-nitrophenyl phosphate + H2O 152.95% activity compared to myo-inositol hexakisphosphate Papiliotrema laurentii ABO 510 p-nitrophenol + phosphate - ? 64623 3.1.3.8 p-nitrophenyl phosphate + H2O - Aspergillus niger N-J p-nitrophenol + phosphate - ? 64623 3.1.3.8 p-nitrophenyl phosphate + H2O the enzyme shows 22.5% activity towards p-nitrophenyl phosphate compared to myo-inositol hexakisphosphate Klebsiella sp. ASR1 p-nitrophenol + phosphate - ? 64623 3.1.3.8 p-nitrophenyl phosphate + H2O the enzyme shows 22.5% activity towards p-nitrophenyl phosphate compared to myo-inositol hexakisphosphate Bacillus amyloliquefaciens DSM 7 p-nitrophenol + phosphate - ? 64623 3.1.3.8 phenyl phosphate - Aspergillus fumigatus phenol + phosphate - ? 364550 3.1.3.8 phenyl phosphate + H2O - Aspergillus niger phenol + phosphate - ? 73499 3.1.3.8 phenyl phosphate + H2O - Aspergillus ficuum phenol + phosphate - ? 73499 3.1.3.8 phenyl phosphate + H2O 84% of the activity with myo-inositol hexakisphosphate Aspergillus niger phenol + phosphate - ? 73499 3.1.3.8 phenyl phosphate + H2O 89.2% of the activity with myo-inositol hexakisphosphate Rhizopus microsporus var. oligosporus phenol + phosphate - ? 73499 3.1.3.8 phenyl phosphate + H2O 188% relative activity compared to myo-inositol hexakisphosphate Nicotiana tabacum phenol + phosphate - ? 73499 3.1.3.8 phosphoenolpyruvate + H2O - Aspergillus fumigatus pyruvate + phosphate - ? 64599 3.1.3.8 phosphoenolpyruvate + H2O 130% activity compared to myo-inositol hexakisphosphate Debaryomyces castellii pyruvate + phosphate - ? 64599 3.1.3.8 phosphoenolpyruvate + H2O 328% relative activity compared to myo-inositol hexakisphosphate Nicotiana tabacum pyruvate + phosphate - ? 64599 3.1.3.8 phosphoenolpyruvate + H2O 92% of the activity with 4-nitrophenylphosphate Arabidopsis thaliana pyruvate + phosphate - ? 64599 3.1.3.8 phosphoenolpyruvate + H2O 130% activity compared to myo-inositol hexakisphosphate Debaryomyces castellii CBS 2923 pyruvate + phosphate - ? 64599 3.1.3.8 phytate + H2O - Hordeum vulgare ? - ? 93352 3.1.3.8 phytate + H2O - Bacillus sp. (in: Bacteria) ? - ? 93352 3.1.3.8 phytate + H2O - Aspergillus ficuum ? - ? 93352 3.1.3.8 phytate + H2O - Lilium longiflorum ? - ? 93352 3.1.3.8 phytate + H2O Na- and Ca-phytate, the first results in higher activity, substrate binding analysis by molecular modelling of phytate inside the active site, residue K77 is able to form two hydrogen bonds with the two phosphate groups on the phytate, whereas in case of K77R, the guanidinium group of arginine is able to form up to 4 H-bonds with the two phosphate groups Bacillus sp. (in: Bacteria) ? - ? 93352 3.1.3.8 phytate + H2O - Bacillus sp. (in: Bacteria) MD2 ? - ? 93352 3.1.3.8 phytate + H2O Na- and Ca-phytate, the first results in higher activity, substrate binding analysis by molecular modelling of phytate inside the active site, residue K77 is able to form two hydrogen bonds with the two phosphate groups on the phytate, whereas in case of K77R, the guanidinium group of arginine is able to form up to 4 H-bonds with the two phosphate groups Bacillus sp. (in: Bacteria) MD2 ? - ? 93352 3.1.3.8 phytate + H2O - Aspergillus niger ? + phosphate - ? 382584 3.1.3.8 phytate + H2O - Escherichia coli ? + phosphate - ? 382584 3.1.3.8 phytate + H2O - Serratia sp. ? + phosphate - ? 382584 3.1.3.8 phytate + H2O - Serratia sp. TN49, ACCC 03909 ? + phosphate - ? 382584 3.1.3.8 polyphosphate + H2O - Glycine max ? - ? 364536 3.1.3.8 pyridoxal phosphate + H2O - Lupinus albus pyridoxal + phosphate - ? 73728 3.1.3.8 riboflavin 5'-phosphate + H2O - Aspergillus niger riboflavin + phosphate - ? 73551 3.1.3.8 rice bran + H2O natural phytate source Pseudomonas sp. ? + phosphate - ? 430672 3.1.3.8 rice bran + H2O natural phytate source Pseudomonas sp. BS10-3 ? + phosphate - ? 430672 3.1.3.8 soybean meal + H2O - Yersinia kristensenii ? + phosphate - ? 412885 3.1.3.8 tetrasodium diphosphate + H2O 6% of the activity with myo-inositol hexakisphosphate Citrobacter braakii ? - ? 364549 3.1.3.8 UTP + H2O 350% relative activity compared to myo-inositol hexakisphosphate Nicotiana tabacum UDP + phosphate - ? 73742