3.1.1.45 3-oxoadipate enol-lactone + H2O Alcaligenes eutrophus JMP134 not a substrate Cupriavidus necator ? - ? 73270 3.1.1.45 3-oxoadipate enol-lactone + H2O Alcaligenes eutrophus 335 and JMP222 Cupriavidus necator ? - ? 73270 3.1.1.45 4-carboxymethylene-but-2-ene-4-olide + H2O third enzyme of the halocatechol branch of the beta-ketoadipate pathway, catalyses the hydrolysis of both, (E) and (Z) dienelactone to maleyl acetate Escherichia coli (2E)-4-oxohex-2-enedioic acid - ? 386142 3.1.1.45 4-carboxymethylene-but-2-ene-4-olide + H2O third enzyme of the halocatechol branch of the beta-ketoadipate pathway, catalyses the hydrolysis of both, (E) and (Z) dienelactone to maleyl acetate Escherichia coli DH5-alpha (2E)-4-oxohex-2-enedioic acid - ? 386142 3.1.1.45 4-carboxymethylenebut-2-en-4-olide + H2O the enzyme is one of the enzymes of the halocatechol branch of the beta-ketoadipate pathway, a complex set of catabolic reactions used by bacteria for utilization of aromatic compounds Pseudomonas knackmussii ? - ? 369686 3.1.1.45 4-carboxymethylenebut-2-en-4-olide + H2O dienelactone hydrolase catalyzes a step in the metabolic conversion of chlorocatechols to beta-ketoadipate Pseudomonas knackmussii ? - ? 369686 3.1.1.45 4-chloromuconolactone + H2O - Pseudomonas reinekei maleylacetate + HCl - ? 398680 3.1.1.45 4-fluoro-muconolactone + H2O - Cupriavidus necator ? - ? 73269 3.1.1.45 4-fluoro-muconolactone + H2O - Pseudomonas knackmussii ? - ? 73269 3.1.1.45 4-fluoro-muconolactone + H2O AC866 Pseudomonas putida ? - ? 73269 3.1.1.45 4-fluoromuconolactone + H2O - Pseudomonas sp. maleylacetate + HF - ? 364274 3.1.1.45 4-fluoromuconolactone + H2O - Pseudomonas sp. MT1 maleylacetate + HF - ? 364274 3.1.1.45 4-nitrophenyl acetate + H2O - Pseudomonas knackmussii 4-nitrophenol + acetate - ? 12514 3.1.1.45 4-nitrophenyl acetate + H2O - Pseudomonas knackmussii DSM 6978 4-nitrophenol + acetate - ? 12514 3.1.1.45 alpha-naphthyl acetate + H2O - Escherichia coli 1-naphthol + acetate - ? 73361 3.1.1.45 alpha-naphthyl acetate + H2O - Escherichia coli DH5-alpha 1-naphthol + acetate - ? 73361 3.1.1.45 azilsartan medoxomil + H2O - Macaca fascicularis azilsartan + ? - ? 429206 3.1.1.45 azilsartan medoxomil + H2O - Homo sapiens azilsartan + ? - ? 429206 3.1.1.45 azilsartan medoxomil + H2O - Mus musculus azilsartan + ? - ? 429206 3.1.1.45 azilsartan medoxomil + H2O - Rattus norvegicus azilsartan + ? - ? 429206 3.1.1.45 azilsartan medoxomil + H2O - Canis lupus familiaris azilsartan + ? - ? 429206 3.1.1.45 azilsartan medoxomil + H2O - Rattus norvegicus Sprague-Dawley azilsartan + ? - ? 429206 3.1.1.45 candesartan cilexetil + H2O - Macaca fascicularis candesartan + ? - ? 429217 3.1.1.45 candesartan cilexetil + H2O - Homo sapiens candesartan + ? - ? 429217 3.1.1.45 candesartan cilexetil + H2O - Mus musculus candesartan + ? - ? 429217 3.1.1.45 candesartan cilexetil + H2O - Rattus norvegicus candesartan + ? - ? 429217 3.1.1.45 candesartan cilexetil + H2O - Canis lupus familiaris candesartan + ? - ? 429217 3.1.1.45 candesartan cilexetil + H2O - Rattus norvegicus Sprague-Dawley candesartan + ? - ? 429217 3.1.1.45 cis-2-chloro-3-methyldienelactone + H2O - Ralstonia sp. 2-chloro-3-methylmaleylacetate - ? 364268 3.1.1.45 cis-2-chloro-5-methyldienelactone + H2O - Ralstonia sp. 2-chloro-5-methylmaleylacetate - ? 364272 3.1.1.45 cis-3-chloro-2-methyldienelactone + H2O - Ralstonia sp. 3-chloro-2-methylmaleylacetate - ? 364270 3.1.1.45 cis-4-carboxymethylenebut-2-chloro-2-en-4-olide + H2O - Rhodococcus opacus ? - ? 73267 3.1.1.45 cis-4-carboxymethylenebut-2-en-4-olide + H2O - Pseudomonas putida 4-oxohex-2-enedioate - ? 73262 3.1.1.45 cis-4-carboxymethylenebut-2-en-4-olide + H2O - Cupriavidus necator 4-oxohex-2-enedioate - ? 73262 3.1.1.45 cis-4-carboxymethylenebut-2-en-4-olide + H2O - Burkholderia cepacia 4-oxohex-2-enedioate - ? 73262 3.1.1.45 cis-4-carboxymethylenebut-2-en-4-olide + H2O - Rhodococcus opacus 4-oxohex-2-enedioate - ? 73262 3.1.1.45 cis-4-carboxymethylenebut-2-en-4-olide + H2O - Pseudomonas knackmussii 4-oxohex-2-enedioate - ? 73262 3.1.1.45 cis-4-carboxymethylenebut-2-en-4-olide + H2O - Pseudomonas knackmussii 4-oxohex-2-enedioate - ir 73262 3.1.1.45 cis-4-carboxymethylenebut-2-en-4-olide + H2O Alcaligenes eutrophus JPM134 Cupriavidus necator 4-oxohex-2-enedioate - ? 73262 3.1.1.45 cis-4-carboxymethylenebut-2-en-4-olide + H2O Pseudomonas putida AC866 Pseudomonas putida 4-oxohex-2-enedioate - ? 73262 3.1.1.45 cis-4-carboxymethylenebut-2-en-4-olide + H2O Alcaligenes eutrophus 335, Alcaligenes eutrophus H16, Alcaligenes eutrophus JMP222: not a substrate to a significant extent Cupriavidus necator 4-oxohex-2-enedioate - ? 73262 3.1.1.45 cis-4-carboxymethylenebut-2-en-4-olide + H2O Pseudomonas putida RW10 not a substrate to a significant extent Pseudomonas putida 4-oxohex-2-enedioate - ? 73262 3.1.1.45 cis-4-carboxymethylenebut-2-en-4-olide + H2O not a substrate to a significant extent Alcaligenes sp. 4-oxohex-2-enedioate - ? 73262 3.1.1.45 cis-4-carboxymethylenebut-2-en-4-olide + H2O not a substrate to a significant extent Alcaligenes sp. A7 4-oxohex-2-enedioate - ? 73262 3.1.1.45 cis-4-carboxymethylenebut-2-methyl-2-en-4-olide + H2O - Rhodococcus opacus ? - ? 73268 3.1.1.45 cis-5-chloro-2-methyldienelactone + H2O - Ralstonia sp. 5-chloro-2-methylmaleylacetate - ? 364266 3.1.1.45 cis-5-chloro-2-methyldienelactone + H2O reaction is part of the degradation of dichloromethylcatechols as central intermediates in the degradation of dichlorotoluenes Ralstonia sp. 5-chloro-2-methylmaleylacetate - ? 364266 3.1.1.45 cis-5-chloro-2-methyldienelactone + H2O - Ralstonia sp. PS12 5-chloro-2-methylmaleylacetate - ? 364266 3.1.1.45 cis-dienelactone + H2O - Cupriavidus necator maleylacetate - ? 364265 3.1.1.45 cis-dienelactone + H2O - Ralstonia sp. maleylacetate - ? 364265 3.1.1.45 cis-dienelactone + H2O best substrate Ralstonia sp. maleylacetate - ? 364265 3.1.1.45 cis-dienelactone + H2O - Ralstonia sp. PS12 maleylacetate - ? 364265 3.1.1.45 cis-dienelactone + H2O best substrate Ralstonia sp. PS12 maleylacetate - ? 364265 3.1.1.45 cis-dienelactone + H2O - Cupriavidus necator JMP 134-1 maleylacetate - ? 364265 3.1.1.45 dienelactone + H2O - Saccharolobus solfataricus maleylacetate - ? 370650 3.1.1.45 dienelactone + H2O enzyme is involved in the degradation of chlorocatechols resulting from growth on from 3-chlorobenzoate and 2,4-dichlorophenoxyacetate Cupriavidus necator maleylacetate - ? 370650 3.1.1.45 dienelactone + H2O - Saccharolobus solfataricus P1 maleylacetate - ? 370650 3.1.1.45 dienelactone + H2O enzyme is involved in the degradation of chlorocatechols resulting from growth on from 3-chlorobenzoate and 2,4-dichlorophenoxyacetate Cupriavidus necator JMP 134-1 maleylacetate - ? 370650 3.1.1.45 E/Z-dienelactone + H2O the reaction is reversible when performed by the mutant enzyme C123S Pseudomonas sp. maleylacetate - ? 364264 3.1.1.45 E/Z-dienelactone + H2O the reaction is reversible when performed by the mutant enzyme C123S Pseudomonas sp. B13 maleylacetate - ? 364264 3.1.1.45 faropenem medoxomil + H2O - Homo sapiens ? - ? 409401 3.1.1.45 lenampicillin + H2O - Homo sapiens ? - ? 409751 3.1.1.45 additional information - Burkholderia cepacia ? - ? 89 3.1.1.45 additional information - Rhodococcus opacus ? - ? 89 3.1.1.45 additional information muconolactone is not hydrolyzed to a significant extent Burkholderia cepacia ? - ? 89 3.1.1.45 additional information muconolactone is not hydrolyzed to a significant extent Pseudomonas knackmussii ? - ? 89 3.1.1.45 additional information DLH unable to hydrolyze lactams which closely resemble its substrate, dienelactam is not a substrate, DLH has lost the ability to hydrolyze amides Pseudomonas knackmussii ? - ? 89 3.1.1.45 additional information substrate specificities differentiate among three types of this activity. Type I: extracts of Alcaligenes eutrophus 335, Alcaligenes eutrophus H16, Alcaligenes eutrophus JMP222 and Alcaligenes strain A7 convert trans-4-carboxymethylenebut-2-en-4-olide, trans-dienelactone, much faster than cis-isomer, type II: enzyme of Pseudomonas cepacia converts cis-dienelactone much faster, type III: enzyme of Alcaligenes eutrophus JMP134 and Pseudomonas strain B13 hydrolyze both dienelactones Cupriavidus necator ? - ? 89 3.1.1.45 additional information substrate specificities differentiate among three types of this activity. Type I: extracts of Alcaligenes eutrophus 335, Alcaligenes eutrophus H16, Alcaligenes eutrophus JMP222 and Alcaligenes strain A7 convert trans-4-carboxymethylenebut-2-en-4-olide, trans-dienelactone, much faster than cis-isomer, type II: enzyme of Pseudomonas cepacia converts cis-dienelactone much faster, type III: enzyme of Alcaligenes eutrophus JMP134 and Pseudomonas strain B13 hydrolyze both dienelactones Alcaligenes sp. ? - ? 89 3.1.1.45 additional information substrate specificities differentiate among three types of this activity. Type I: extracts of Alcaligenes eutrophus 335, Alcaligenes eutrophus H16, Alcaligenes eutrophus JMP222 and Alcaligenes strain A7 convert trans-4-carboxymethylenebut-2-en-4-olide, trans-dienelactone, much faster than cis-isomer, type II: enzyme of Pseudomonas cepacia converts cis-dienelactone much faster, type III: enzyme of Alcaligenes eutrophus JMP134 and Pseudomonas strain B13 hydrolyze both dienelactones Burkholderia cepacia ? - ? 89 3.1.1.45 additional information substrate specificities differentiate among three types of this activity. Type I: extracts of Alcaligenes eutrophus 335, Alcaligenes eutrophus H16, Alcaligenes eutrophus JMP222 and Alcaligenes strain A7 convert trans-4-carboxymethylenebut-2-en-4-olide, trans-dienelactone, much faster than cis-isomer, type II: enzyme of Pseudomonas cepacia converts cis-dienelactone much faster, type III: enzyme of Alcaligenes eutrophus JMP134 and Pseudomonas strain B13 hydrolyze both dienelactones Pseudomonas knackmussii ? - ? 89 3.1.1.45 additional information substrate binding studies Pseudomonas knackmussii ? - ? 89 3.1.1.45 additional information no activity with 2-methylmuconolactone, 4-methylmuconolactone Rhodococcus opacus ? - ? 89 3.1.1.45 additional information antibiotic protoanemonin is a poor substrate Pseudomonas sp. ? - ? 89 3.1.1.45 additional information antibiotic protoanemonin is a poor substrate Ralstonia sp. ? - ? 89 3.1.1.45 additional information the mutant C123S catalyzes the isomerase reaction which changes the E-isomer to Z-isomer and vice versa Pseudomonas sp. ? - ? 89 3.1.1.45 additional information natural function is not in the degradation of haloaromatic compounds, the normal substrate might well be one of the more complex natural compounds with a dienelactone structure, or it could also be an intermediate of some other metabolic pathway Burkholderia cepacia ? - ? 89 3.1.1.45 additional information it seems likely, that dienelactone hydrolase has the additional function of detoxification of minor amounts of protoanemonin that may be formed during chloroaromatic degradation Pseudomonas knackmussii ? - ? 89 3.1.1.45 additional information antibiotic protoanemonin is no substrate Pseudomonas sp. ? - ? 89 3.1.1.45 additional information pathway overview Ralstonia sp. ? - ? 89 3.1.1.45 additional information the enzyme is involved in degradation of 4-chlorobenzenesulfonic acid, 4CBSA, the major polar by-product of the chemical synthesis of 1,1,1-trichloro-2,2-bis-(4-chlorophenyl) ethane, DDT, overview, 4-chlorobenzenesulfonic acid catabolic pathway, overview Pseudomonas aeruginosa ? - ? 89 3.1.1.45 additional information no activity with protoanemonin Pseudomonas aeruginosa ? - ? 89 3.1.1.45 additional information the enzyme is a typical dienelactone hydrolase belonging to alpha/beta hydrolase family and containing a catalytic triad composed of Cys151, Asp198, and His229 in the active site Saccharolobus solfataricus ? - ? 89 3.1.1.45 additional information the enzyme also shows carboxylesterase activity toward 4-nitrophenyl esters ranging from butyrate to laurate Saccharolobus solfataricus ? - ? 89 3.1.1.45 additional information the activity with prodrug-type angiotensin II type 1 receptor blocker differs in a tissue-dependent and subcellular-dependent manner, overview Macaca fascicularis ? - ? 89 3.1.1.45 additional information the activity with prodrug-type angiotensin II type 1 receptor blocker differs in a tissue-dependent and subcellular-dependent manner, overview Homo sapiens ? - ? 89 3.1.1.45 additional information the activity with prodrug-type angiotensin II type 1 receptor blocker differs in a tissue-dependent and subcellular-dependent manner, overview Mus musculus ? - ? 89 3.1.1.45 additional information the activity with prodrug-type angiotensin II type 1 receptor blocker differs in a tissue-dependent and subcellular-dependent manner, overview Rattus norvegicus ? - ? 89 3.1.1.45 additional information the activity with prodrug-type angiotensin II type 1 receptor blocker differs in a tissue-dependent and subcellular-dependent manner, overview Canis lupus familiaris ? - ? 89 3.1.1.45 additional information the activity with prodrug-type angiotensin II type 1 receptor blocker differs in a tissue-dependent and subcellular-dependent manner, overview Rattus norvegicus Sprague-Dawley ? - ? 89 3.1.1.45 additional information the enzyme is a typical dienelactone hydrolase belonging to alpha/beta hydrolase family and containing a catalytic triad composed of Cys151, Asp198, and His229 in the active site Saccharolobus solfataricus P1 ? - ? 89 3.1.1.45 additional information the enzyme also shows carboxylesterase activity toward 4-nitrophenyl esters ranging from butyrate to laurate Saccharolobus solfataricus P1 ? - ? 89 3.1.1.45 additional information the mutant C123S catalyzes the isomerase reaction which changes the E-isomer to Z-isomer and vice versa Pseudomonas sp. B13 ? - ? 89 3.1.1.45 additional information antibiotic protoanemonin is a poor substrate Pseudomonas sp. MT1 ? - ? 89 3.1.1.45 additional information antibiotic protoanemonin is no substrate Pseudomonas sp. MT1 ? - ? 89 3.1.1.45 additional information antibiotic protoanemonin is a poor substrate Ralstonia sp. PS12 ? - ? 89 3.1.1.45 additional information pathway overview Ralstonia sp. PS12 ? - ? 89 3.1.1.45 additional information substrate specificities differentiate among three types of this activity. Type I: extracts of Alcaligenes eutrophus 335, Alcaligenes eutrophus H16, Alcaligenes eutrophus JMP222 and Alcaligenes strain A7 convert trans-4-carboxymethylenebut-2-en-4-olide, trans-dienelactone, much faster than cis-isomer, type II: enzyme of Pseudomonas cepacia converts cis-dienelactone much faster, type III: enzyme of Alcaligenes eutrophus JMP134 and Pseudomonas strain B13 hydrolyze both dienelactones Alcaligenes sp. A7 ? - ? 89 3.1.1.45 olmesartan medoxomil + H2O - Homo sapiens ? - ? 410014 3.1.1.45 olmesartan medoxomil + H2O - Macaca fascicularis olmesartan + diacetyl - ? 429357 3.1.1.45 olmesartan medoxomil + H2O - Homo sapiens olmesartan + diacetyl - ? 429357 3.1.1.45 olmesartan medoxomil + H2O - Mus musculus olmesartan + diacetyl - ? 429357 3.1.1.45 olmesartan medoxomil + H2O - Rattus norvegicus olmesartan + diacetyl - ? 429357 3.1.1.45 olmesartan medoxomil + H2O - Canis lupus familiaris olmesartan + diacetyl - ? 429357 3.1.1.45 olmesartan medoxomil + H2O - Rattus norvegicus Sprague-Dawley olmesartan + diacetyl - ? 429357 3.1.1.45 p-nitrophenyl acetate + H2O - Escherichia coli p-nitrophenol + acetate - ? 36053 3.1.1.45 p-nitrophenyl acetate + H2O esterase activity Pseudomonas knackmussii p-nitrophenol + acetate - ? 36053 3.1.1.45 p-nitrophenyl acetate + H2O - Escherichia coli DH5-alpha p-nitrophenol + acetate - ? 36053 3.1.1.45 protoanemonin + H2O - Pseudomonas knackmussii cis-acetylacrylate - ? 73264 3.1.1.45 protoanemonin + H2O activity of cis-dienelactone hydrolase with 50 microM protoanemonin is 0.08% of that observed with 50 microM cis-dienelactone, cis-4-carboxymethylenebut-2-en-4-olide Burkholderia cepacia cis-acetylacrylate - ? 73264 3.1.1.45 trans-2-chloro-3-methyldienelactone + H2O - Ralstonia sp. 2-chloro-3-methylmaleylacetate - ? 364269 3.1.1.45 trans-3-chloro-2-methyldienelactone + H2O - Ralstonia sp. 3-chloro-2-methylmaleylacetate - ? 364271 3.1.1.45 trans-4-carboxymethylenebut-2-en-4-olide + H2O - Pseudomonas putida 4-oxohex-2-enedioate - ? 73263 3.1.1.45 trans-4-carboxymethylenebut-2-en-4-olide + H2O - Cupriavidus necator 4-oxohex-2-enedioate - ? 73263 3.1.1.45 trans-4-carboxymethylenebut-2-en-4-olide + H2O - Alcaligenes sp. 4-oxohex-2-enedioate - ? 73263 3.1.1.45 trans-4-carboxymethylenebut-2-en-4-olide + H2O - Pseudomonas knackmussii 4-oxohex-2-enedioate - ? 73263 3.1.1.45 trans-4-carboxymethylenebut-2-en-4-olide + H2O - Pseudomonas knackmussii 4-oxohex-2-enedioate - ir 73263 3.1.1.45 trans-4-carboxymethylenebut-2-en-4-olide + H2O not a substrate to a significant extent Burkholderia cepacia 4-oxohex-2-enedioate - ? 73263 3.1.1.45 trans-4-carboxymethylenebut-2-en-4-olide + H2O not a substrate to a significant extent Rhodococcus opacus 4-oxohex-2-enedioate - ? 73263 3.1.1.45 trans-4-carboxymethylenebut-2-en-4-olide + H2O - Alcaligenes sp. A7 4-oxohex-2-enedioate - ? 73263 3.1.1.45 trans-5-chloro-2-methyldienelactone + H2O - Ralstonia sp. 5-chloro-2-methylmaleylacetate - ? 364267 3.1.1.45 trans-cinnamoyl imidazole + H2O esterase activity Pseudomonas knackmussii trans-cinnamate + imidazole - ? 73266 3.1.1.45 trans-dienelactone + H2O - Pseudomonas sp. maleylacetate - ? 364273 3.1.1.45 trans-dienelactone + H2O - Pseudomonas reinekei maleylacetate - ? 364273 3.1.1.45 trans-dienelactone + H2O very low activity with the cis-isomer Pseudomonas sp. maleylacetate - ? 364273 3.1.1.45 trans-dienelactone + H2O degradation of chlorosalicylates via chlorocatechols to 3-oxoadipate via maleylacetate, pathway overview Pseudomonas sp. maleylacetate - ? 364273 3.1.1.45 trans-dienelactone + H2O specific for the trans-isomer, no activity with the cis-isomer Pseudomonas aeruginosa maleylacetate - ? 364273 3.1.1.45 trans-dienelactone + H2O the enzyme displays substrate specificities toward trans-dienelactone, not cis-isomer Saccharolobus solfataricus maleylacetate - ? 364273 3.1.1.45 trans-dienelactone + H2O the enzyme displays substrate specificities toward trans-dienelactone, not cis-isomer Saccharolobus solfataricus P1 maleylacetate - ? 364273 3.1.1.45 trans-dienelactone + H2O - Pseudomonas sp. B13 maleylacetate - ? 364273 3.1.1.45 trans-dienelactone + H2O very low activity with the cis-isomer Pseudomonas sp. MT1 maleylacetate - ? 364273 3.1.1.45 trans-dienelactone + H2O degradation of chlorosalicylates via chlorocatechols to 3-oxoadipate via maleylacetate, pathway overview Pseudomonas sp. MT1 maleylacetate - ? 364273 3.1.1.45 Z-dienelactone + H2O - Pseudomonas sp. maleyl acetate - ? 453959 3.1.1.45 Z-dienelactone + H2O - Pseudomonas sp. B13 maleyl acetate - ? 453959