3.1.1.3 4-nitrophenyl acetate + H2O about 30% activity compared to 4-nitrophenyl caprate Acinetobacter baylyi 4-nitrophenol + acetate - ? 12514 3.1.1.3 4-nitrophenyl butyrate + H2O about 65% activity compared to 4-nitrophenyl caprate Acinetobacter baylyi 4-nitrophenol + butyrate - ? 115376 3.1.1.3 4-nitrophenyl caprate + H2O best substrate Acinetobacter baylyi 4-nitrophenol + caprate - ? 364083 3.1.1.3 4-nitrophenyl caprylate + H2O about 85% activity compared to 4-nitrophenyl caprate Acinetobacter baylyi 4-nitrophenol + caprylate - ? 363889 3.1.1.3 4-nitrophenyl laurate + H2O about 90% activity compared to 4-nitrophenyl caprate Acinetobacter baylyi 4-nitrophenol + laurate - ? 363891 3.1.1.3 4-nitrophenyl myristate + H2O about 50% activity compared to 4-nitrophenyl caprate Acinetobacter baylyi 4-nitrophenol + myristate - ? 364082 3.1.1.3 4-nitrophenyl palmitate + H2O about 40% activity compared to 4-nitrophenyl caprate Acinetobacter baylyi 4-nitrophenol + palmitate - ? 363890 3.1.1.3 4-nitrophenyl stearate + H2O about 35% activity compared to 4-nitrophenyl caprate Acinetobacter baylyi 4-nitrophenol + stearate - ? 364135 3.1.1.3 additional information the enzyme 11folds favorably catalyzes the hydrolysis of rice bran oil, sesame oil, and coconut oil in comparison to palm oil. The transesterification activity of palm oil to fatty acid methyl esters reveals 31.64% after 48h. Wasting palm oil shows the highest activity among all oil substrates tested (about 1300% activity compared to palm oil). COmpared to palm oil, the enzyme shows about 150% activity with sunflower oil, about 150% activity with safflower oil, about 300% activity with canola oil, about 300% activity with olive oil, about 100% activity with soybean oil, and about 600% activity with camellia tea oil Acinetobacter baylyi ? - ? 89 3.1.1.3 1,2-dipalmitin + H2O - Acinetobacter calcoaceticus ? - ? 64424 3.1.1.3 1-monopalmitin + H2O - Acinetobacter calcoaceticus palmitic acid + glycerol - ? 64425 3.1.1.3 4-nitrophenyl palmitate + H2O - Acinetobacter calcoaceticus 4-nitrophenol + palmitate - ? 363890 3.1.1.3 additional information maximal activity with fatty acid chain length of 14 carbon atoms, similarly active with the diacylglycerols and triacylglycerols and more active with oleic acid than with stearic acid Acinetobacter calcoaceticus ? - ? 89 3.1.1.3 additional information substrates are long acyl chain 4-nitrophenol esters Acinetobacter calcoaceticus ? - ? 89 3.1.1.3 tributyrin + H2O - Acinetobacter calcoaceticus dibutyrin + butyrate - ? 363949 3.1.1.3 trimyristin + H2O - Acinetobacter calcoaceticus myristic acid + ? - ? 64401 3.1.1.3 trioleoylglycerol + H2O - Acinetobacter calcoaceticus oleic acid + ? - ? 64389 3.1.1.3 tripalmitin + H2O - Acinetobacter calcoaceticus palmitic acid + ? - ? 64402 3.1.1.3 tristearin + H2O - Acinetobacter calcoaceticus stearic acid + ? - ? 64403 3.1.1.3 additional information maximal activity with fatty acid chain length of 14 carbon atoms, similarly active with the diacylglycerols and triacylglycerols and more active with oleic acid than with stearic acid Acinetobacter calcoaceticus 69 V ? - ? 89 3.1.1.3 trimyristin + H2O - Acinetobacter calcoaceticus 69 V myristic acid + ? - ? 64401 3.1.1.3 trioleoylglycerol + H2O - Acinetobacter calcoaceticus 69 V oleic acid + ? - ? 64389 3.1.1.3 tripalmitin + H2O - Acinetobacter calcoaceticus 69 V palmitic acid + ? - ? 64402 3.1.1.3 tristearin + H2O - Acinetobacter calcoaceticus 69 V stearic acid + ? - ? 64403 3.1.1.3 4-nitrophenyl palmitate + H2O - Acinetobacter calcoaceticus BD 413 4-nitrophenol + palmitate - ? 363890 3.1.1.3 additional information substrates are long acyl chain 4-nitrophenol esters Acinetobacter calcoaceticus BD 413 ? - ? 89 3.1.1.3 tributyrin + H2O - Acinetobacter calcoaceticus BD 413 dibutyrin + butyrate - ? 363949 3.1.1.3 4-nitrophenyl acetate + H2O low activity Acinetobacter sp. 4-nitrophenol + acetate - ? 12514 3.1.1.3 4-nitrophenyl acetate + H2O highest activity with 4-nitrophenyl acetate Acinetobacter sp. 4-nitrophenol + acetate - ? 12514 3.1.1.3 4-nitrophenyl butyrate + H2O - Acinetobacter sp. 4-nitrophenol + butyrate - ? 115376 3.1.1.3 4-nitrophenyl decanoate + H2O - Acinetobacter sp. 4-nitrophenol + decanoate - ? 360778 3.1.1.3 4-nitrophenyl hexanoate + H2O best substrate Acinetobacter sp. 4-nitrophenol + hexanoate - ? 115379 3.1.1.3 4-nitrophenyl laurate + H2O - Acinetobacter sp. 4-nitrophenol + laurate - ? 363891 3.1.1.3 4-nitrophenyl myristate + H2O - Acinetobacter sp. 4-nitrophenol + myristate - ? 364082 3.1.1.3 4-nitrophenyl octanoate + H2O best substrate Acinetobacter sp. 4-nitrophenol + octanoate - ? 115380 3.1.1.3 4-nitrophenyl palmitate + H2O - Acinetobacter sp. 4-nitrophenol + palmitate - ? 363890 3.1.1.3 4-nitrophenyl stearate + H2O - Acinetobacter sp. 4-nitrophenol + stearate - ? 364135 3.1.1.3 additional information substrate specificity, enzyme prefers substrates of medium- and long-chain fatty acids Acinetobacter sp. ? - ? 89 3.1.1.3 additional information broad specificity towards the acyl group (C8-C16) of ethyl esters Acinetobacter sp. ? - ? 89 3.1.1.3 mustard oil + H2O - Acinetobacter sp. ? - ? 364072 3.1.1.3 olive oil + H2O - Acinetobacter sp. ? - ? 364063 3.1.1.3 soybean oil + H2O - Acinetobacter sp. ? - ? 364064 3.1.1.3 tributyrin + H2O - Acinetobacter sp. dibutyrin + butyrate - ? 363949 3.1.1.3 Tween 80 + H2O - Acinetobacter sp. ? - ? 364144 3.1.1.3 4-nitrophenyl acetate + H2O highest activity with 4-nitrophenyl acetate Acinetobacter sp. CR9 4-nitrophenol + acetate - ? 12514 3.1.1.3 mustard oil + H2O - Acinetobacter sp. CR9 ? - ? 364072 3.1.1.3 olive oil + H2O - Acinetobacter sp. CR9 ? - ? 364063 3.1.1.3 soybean oil + H2O - Acinetobacter sp. CR9 ? - ? 364064 3.1.1.3 tributyrin + H2O - Acinetobacter sp. CR9 dibutyrin + butyrate - ? 363949 3.1.1.3 4-nitrophenyl laurate + H2O - Acinetobacter sp. MTCC 6816 4-nitrophenol + laurate - ? 363891 3.1.1.3 olive oil + H2O - Acinetobacter sp. MTCC 6816 ? - ? 364063 3.1.1.3 4-nitrophenyl acetate + H2O low activity Acinetobacter sp. RAG-1 4-nitrophenol + acetate - ? 12514 3.1.1.3 4-nitrophenyl butyrate + H2O - Acinetobacter sp. RAG-1 4-nitrophenol + butyrate - ? 115376 3.1.1.3 4-nitrophenyl hexanoate + H2O best substrate Acinetobacter sp. RAG-1 4-nitrophenol + hexanoate - ? 115379 3.1.1.3 4-nitrophenyl octanoate + H2O best substrate Acinetobacter sp. RAG-1 4-nitrophenol + octanoate - ? 115380 3.1.1.3 additional information substrate specificity, enzyme prefers substrates of medium- and long-chain fatty acids Acinetobacter sp. RAG-1 ? - ? 89 3.1.1.3 additional information medium chain acyl group p-nitrophenyl esters are good substrate, increased activity with detergents Aeromonas sp. ? - ? 89 3.1.1.3 additional information medium chain acyl group p-nitrophenyl esters are good substrate, increased activity with detergents Aeromonas sp. LPB 4 ? - ? 89 3.1.1.3 (R,S)-mandelonitrile + vinyl acetate transesterification Alcaligenes sp. (S)-cyano(phenyl)methyl acetate + (R)-mandelonitrite - ? 399193 3.1.1.3 additional information enzyme does not possess protease activity and does not hydrolyze peptide bonds, no activity with N-benzoyl-L-tyrosine 4-nitroanilide Alcaligenes ssp. ? - ? 89 3.1.1.3 tributyrin + H2O - Alcaligenes ssp. dibutyrin + butyrate - ? 363949 3.1.1.3 4 triolein + H2O about 115% activity compared to olive oil Amycolatopsis mediterranei 6 oleic acid + 1,3-diolein + 1,2-diolein + 1-monoolein + 2-monoolein oleic acid is the major product ? 417723 3.1.1.3 4-nitrophenyl acetate + H2O about 88% activity compared to 4-nitrophenyl palmitate Amycolatopsis mediterranei 4-nitrophenol + acetate - ? 12514 3.1.1.3 4-nitrophenyl butyrate + H2O about 90% activity compared to 4-nitrophenyl palmitate Amycolatopsis mediterranei 4-nitrophenol + butyrate - ? 115376 3.1.1.3 4-nitrophenyl caproate + H2O second-highest hydrolysis rate, about 95% activity compared to 4-nitrophenyl palmitate Amycolatopsis mediterranei 4-nitrophenol + caproate - ? 363887 3.1.1.3 4-nitrophenyl caprylate + H2O highest hydrolysis rate, about 90% activity compared to 4-nitrophenyl palmitate Amycolatopsis mediterranei 4-nitrophenol + caprylate - ? 363889 3.1.1.3 4-nitrophenyl laurate + H2O about 60% activity compared to 4-nitrophenyl palmitate Amycolatopsis mediterranei 4-nitrophenol + laurate - ? 363891 3.1.1.3 4-nitrophenyl myristate + H2O about 30% activity compared to 4-nitrophenyl palmitate Amycolatopsis mediterranei 4-nitrophenol + myristate - ? 364082 3.1.1.3 4-nitrophenyl palmitate + H2O the purified enzyme is most active toward 4-nitrophenyl palmitate (100% activity) Amycolatopsis mediterranei 4-nitrophenol + palmitate - ? 363890 3.1.1.3 isoamyl alcohol + acetic acid after 72 h of reaction a conversion yield of 34.4% and 16.2% of isoamyl acetate is obtained using cellite-immobilized and free enzyme, respectively Amycolatopsis mediterranei isoamyl acetate - ? 417110 3.1.1.3 additional information the enzyme also uses olive oil (100% activity), corn oil (about 85% activity), castor oil (about 80% activity), sunflower oil (about 95% activity), rape seed oil (about 85% activity), linseed oil (about 75% activity), cotton seed (about 90% activity), and jojoba oil (about 83% activity) as substrates Amycolatopsis mediterranei ? - ? 89 3.1.1.3 trilinolein + H2O about 105% activity compared to olive oil Amycolatopsis mediterranei ? - ? 417720 3.1.1.3 trilinolenin + H2O about 100% activity compared to olive oil Amycolatopsis mediterranei ? - ? 417721 3.1.1.3 trioctanoin + H2O about 59% activity compared to olive oil Amycolatopsis mediterranei ? - ? 64460 3.1.1.3 tripalmitin + H2O about 45% activity compared to olive oil Amycolatopsis mediterranei ? - ? 413001 3.1.1.3 tristearin + H2O about 38% activity compared to olive oil Amycolatopsis mediterranei ? - ? 413004 3.1.1.3 trivaccinin + H2O about 78% activity compared to olive oil Amycolatopsis mediterranei ? - ? 417726 3.1.1.3 4-nitrophenyl acetate + H2O about 88% activity compared to 4-nitrophenyl palmitate Amycolatopsis mediterranei DSM 43304 4-nitrophenol + acetate - ? 12514 3.1.1.3 4-nitrophenyl butyrate + H2O about 90% activity compared to 4-nitrophenyl palmitate Amycolatopsis mediterranei DSM 43304 4-nitrophenol + butyrate - ? 115376 3.1.1.3 4-nitrophenyl caproate + H2O second-highest hydrolysis rate, about 95% activity compared to 4-nitrophenyl palmitate Amycolatopsis mediterranei DSM 43304 4-nitrophenol + caproate - ? 363887 3.1.1.3 additional information the enzyme also uses olive oil (100% activity), corn oil (about 85% activity), castor oil (about 80% activity), sunflower oil (about 95% activity), rape seed oil (about 85% activity), linseed oil (about 75% activity), cotton seed (about 90% activity), and jojoba oil (about 83% activity) as substrates Amycolatopsis mediterranei DSM 43304 ? - ? 89 3.1.1.3 trioctanoin + H2O about 59% activity compared to olive oil Amycolatopsis mediterranei DSM 43304 ? - ? 64460 3.1.1.3 1,3-diolein + H2O low activity Arabidopsis thaliana 1-oleoyl-sn-glycerol + oleate - ? 380833 3.1.1.3 additional information the enzyme is involved in but not essential for breakdoen of stored triacylglycerides during germination Arabidopsis thaliana ? - ? 89 3.1.1.3 additional information no activity with olein, digalactosyldiacylglycerol, galactosyldiacylglycerol, phosphatidylcholine, and cholesterol oleate Arabidopsis thaliana ? - ? 89 3.1.1.3 tributyrin + H2O preferred substrate Arabidopsis thaliana dibutyrin + butyrate - ? 363949 3.1.1.3 tricaprylin + H2O good substrate Arabidopsis thaliana dicaprylin + caprylate - ? 364128 3.1.1.3 triolein + H2O - Arabidopsis thaliana diolein + oleate - ? 363933 3.1.1.3 triolein + H2O low activity Arabidopsis thaliana diolein + oleate - ? 363933 3.1.1.3 4-nitrophenyl octanoate + H2O - Archaeoglobus fulgidus 4-nitrophenol + octanoate - ? 115380 3.1.1.3 4-nitrophenyl palmitate + H2O - Archaeoglobus fulgidus 4-nitrophenol + palmitate - ? 363890 3.1.1.3 tricaprylin + 3 H2O the apparent rate of hydrolysis correlates with the degree of micellar formation. Consistent with observations from other lipases, the lid conformation may change from the closed to the open form in the presence of lipid interface. Together with the evidence of the lid structure in in this enzyme, it is classified as a lipase rather than as a carboxylesterase Archaeoglobus fulgidus 3 octanoate + glycerol - ? 424128 3.1.1.3 (S,R)-acetic acid 2-methyl-4-oxo-3-prop-2-ynyl-cyclopent-2-enyl ester + H2O - Arthrobacter sp. (R)-4-hydroxy-3-methyl-2-prop-2-ynyl-cyclopent-2-enone + (S)-acetic acid 2-methyl-4-oxo-3-prop-2-ynyl-cyclopent-2-enyl ester + acetate - ? 375482 3.1.1.3 4-nitrophenyl acetate + H2O low activity Aspergillus carneus 4-nitrophenol + acetate - ? 12514 3.1.1.3 4-nitrophenyl caproate + H2O - Aspergillus carneus 4-nitrophenol + caproate - ? 363887 3.1.1.3 4-nitrophenyl laurate + H2O enzyme shows a preference for lauric acid and a 1,3-position specificity Aspergillus carneus 4-nitrophenol + laurate - ? 363891 3.1.1.3 4-nitrophenyl myristate + H2O - Aspergillus carneus 4-nitrophenol + myristate - ? 364082 3.1.1.3 4-nitrophenyl palmitate + H2O - Aspergillus carneus 4-nitrophenol + palmitate - ? 363890 3.1.1.3 4-nitrophenyl stearate + H2O low activity Aspergillus carneus 4-nitrophenol + stearate - ? 364135 3.1.1.3 additional information the enzyme utilizes olive oil as a substrate, no activity with 2-nitrophenyl palmitate Aspergillus carneus ? - ? 89 3.1.1.3 4-nitrophenyl acetate + H2O 100% activity Aspergillus fumigatus 4-nitrophenol + acetate - ? 12514 3.1.1.3 4-nitrophenyl butyrate + H2O about 65% activity compared to 4-nitrophenyl acetate Aspergillus fumigatus 4-nitrophenol + butyrate - ? 115376 3.1.1.3 4-nitrophenyl decanoate + H2O about 10% activity compared to 4-nitrophenyl acetate Aspergillus fumigatus 4-nitrophenol + decanoate - ? 360778 3.1.1.3 4-nitrophenyl laurate + H2O - Aspergillus fumigatus 4-nitrophenol + laurate - ? 363891 3.1.1.3 4-nitrophenyl myristate + H2O worst substrate, less than 5% activity compared to 4-nitrophenyl acetate Aspergillus fumigatus 4-nitrophenol + myristate - ? 364082 3.1.1.3 4-nitrophenyl propionate + H2O about 60% activity compared to 4-nitrophenyl acetate Aspergillus fumigatus 4-nitrophenol + propionate - ? 115374 3.1.1.3 additional information AFL1-1 has potential enantioselectivity for the ability to catalyze methyl 3-(4-methoxyphenyl) glycidate and methyl 3-phenylglycidate Aspergillus fumigatus ? - ? 89 3.1.1.3 additional information almost no activity on long-chained acyl esters such as 4-nitrophenyl palmitate Aspergillus fumigatus ? - ? 89 3.1.1.3 4-nitrophenyl acetate + H2O 100% activity Aspergillus fumigatus CGMCC 2873 4-nitrophenol + acetate - ? 12514 3.1.1.3 4-nitrophenyl butyrate + H2O about 65% activity compared to 4-nitrophenyl acetate Aspergillus fumigatus CGMCC 2873 4-nitrophenol + butyrate - ? 115376 3.1.1.3 4-nitrophenyl propionate + H2O about 60% activity compared to 4-nitrophenyl acetate Aspergillus fumigatus CGMCC 2873 4-nitrophenol + propionate - ? 115374 3.1.1.3 additional information AFL1-1 has potential enantioselectivity for the ability to catalyze methyl 3-(4-methoxyphenyl) glycidate and methyl 3-phenylglycidate Aspergillus fumigatus CGMCC 2873 ? - ? 89 3.1.1.3 additional information almost no activity on long-chained acyl esters such as 4-nitrophenyl palmitate Aspergillus fumigatus CGMCC 2873 ? - ? 89 3.1.1.3 4-nitrophenyl palmitate + H2O - Aspergillus nidulans 4-nitrophenol + palmitate - ? 363890 3.1.1.3 additional information substrate specificity Aspergillus nidulans ? - ? 89 3.1.1.3 additional information preference toward esters of short- and middle-chain fatty acids Aspergillus nidulans ? - ? 89 3.1.1.3 4-nitrophenyl palmitate + H2O - Aspergillus nidulans WG312 4-nitrophenol + palmitate - ? 363890 3.1.1.3 additional information preference toward esters of short- and middle-chain fatty acids Aspergillus nidulans WG312 ? - ? 89 3.1.1.3 additional information substrate specificity Aspergillus nidulans WG312 ? - ? 89 3.1.1.3 4-nitrophenyl palmitate + H2O - Aspergillus niger 4-nitrophenol + palmitate - ? 363890 3.1.1.3 high linoleic sunflower oil + H2O - Aspergillus niger ? - ? 401143 3.1.1.3 high oleic sunflower oil + H2O - Aspergillus niger ? - ? 401146 3.1.1.3 methyl beta-D-glucopyranoside + H2O regioselective deacetylation of preacylated substrate Aspergillus niger methanol + beta-D-glucose - ? 364106 3.1.1.3 additional information no activity with trielaidin, an olive oil emulsion containing 10% v/v olive oil and 1% m/v gum arabic is used as substrate, the enzyme shows a sn-1 selectivity using diacylglycerols, and R-isomer hydrolytic preference with pseudolipids representing triacylglycerols in which 2 of the ester bonds are replaced with ether and amide linkages Aspergillus niger ? - ? 89 3.1.1.3 additional information substrate is olive oil, the enzyme is 1,3-specific Aspergillus niger ? - ? 89 3.1.1.3 additional information the enzyme also shows transesterification activity with 4-nitrophenyl palmitate ester substrates, overview Aspergillus niger ? - ? 89 3.1.1.3 olive oil + H2O - Aspergillus niger ? - ? 364063 3.1.1.3 triacetin + H2O - Aspergillus niger diacetin + acetate - ? 364163 3.1.1.3 tributyrin + H2O - Aspergillus niger dibutyrin + butyrate - ? 363949 3.1.1.3 tricaproin + H2O - Aspergillus niger dicaproin + caproate - ? 364147 3.1.1.3 tricaprylin + H2O best substrate Aspergillus niger dicaprylin + caprylate - ? 364128 3.1.1.3 trilinolein + H2O trans C18:1(9) Aspergillus niger dilinolein + linoleate - ? 364139 3.1.1.3 triolein + H2O cis-C18:1(9) Aspergillus niger diolein + oleate - ? 363933 3.1.1.3 triolein + H2O - Aspergillus niger 1,2-diolein + oleate - ? 393714 3.1.1.3 4-nitrophenyl palmitate + H2O - Aspergillus niger NCIM 1207 4-nitrophenol + palmitate - ? 363890 3.1.1.3 triolein + H2O - Aspergillus niger NCIM 1207 1,2-diolein + oleate - ? 393714 3.1.1.3 additional information no activity with trielaidin, an olive oil emulsion containing 10% v/v olive oil and 1% m/v gum arabic is used as substrate, the enzyme shows a sn-1 selectivity using diacylglycerols, and R-isomer hydrolytic preference with pseudolipids representing triacylglycerols in which 2 of the ester bonds are replaced with ether and amide linkages Aspergillus niger Tiegh ? - ? 89 3.1.1.3 tributyrin + H2O - Aspergillus niger Tiegh dibutyrin + butyrate - ? 363949 3.1.1.3 tricaprylin + H2O best substrate Aspergillus niger Tiegh dicaprylin + caprylate - ? 364128 3.1.1.3 trilinolein + H2O trans C18:1(9) Aspergillus niger Tiegh dilinolein + linoleate - ? 364139 3.1.1.3 triolein + H2O cis-C18:1(9) Aspergillus niger Tiegh diolein + oleate - ? 363933 3.1.1.3 (RS)-1-phenylethanol + vinyl acetate enantioselective esterification in vivo Aspergillus oryzae (R)-1-phenylethyl acetate + acetaldehyde + (S)-1-phenylethanol - ir 380436 3.1.1.3 (RS)-1-phenylethanol + vinyl acetate enantioselective esterification Aspergillus oryzae (R)-1-phenylethyl acetate + acetaldehyde + (S)-1-phenylethanol - ir 380436 3.1.1.3 tributyrin + H2O - Aspergillus oryzae dibutyrin + butyrate - ? 363949 3.1.1.3 triolein + H2O preferred substrate of enzyme form L1 Aspergillus oryzae diolein + oleate - ? 363933 3.1.1.3 4-nitrophenyl laurate + H2O - Aureobasidium pullulans 4-nitrophenol + laurate - ? 363891 3.1.1.3 lard + H2O - Aureobasidium pullulans ? - ? 392466 3.1.1.3 olive oil + H2O - Aureobasidium pullulans ? - ? 364063 3.1.1.3 peanut oil + H2O - Aureobasidium pullulans ? - ? 401627 3.1.1.3 peanut oil + H2O highest hydrolytic activity towards peanut oil Aureobasidium pullulans ? - ? 401627 3.1.1.3 soybean oil + H2O - Aureobasidium pullulans ? - ? 364064 3.1.1.3 4-nitrophenyl laurate + H2O - Aureobasidium pullulans HN2-3 4-nitrophenol + laurate - ? 363891 3.1.1.3 lard + H2O - Aureobasidium pullulans HN2-3 ? - ? 392466 3.1.1.3 olive oil + H2O - Aureobasidium pullulans HN2-3 ? - ? 364063 3.1.1.3 peanut oil + H2O - Aureobasidium pullulans HN2-3 ? - ? 401627 3.1.1.3 soybean oil + H2O - Aureobasidium pullulans HN2-3 ? - ? 364064 3.1.1.3 4-nitrophenyl laurate + H2O - Aureobasidium pullulans HN2.3 4-nitrophenol + laurate - ? 363891 3.1.1.3 lard + H2O - Aureobasidium pullulans HN2.3 ? - ? 392466 3.1.1.3 olive oil + H2O - Aureobasidium pullulans HN2.3 ? - ? 364063 3.1.1.3 peanut oil + H2O highest hydrolytic activity towards peanut oil Aureobasidium pullulans HN2.3 ? - ? 401627 3.1.1.3 soybean oil + H2O - Aureobasidium pullulans HN2.3 ? - ? 364064 3.1.1.3 (R,S)-flurbiprofen ethyl ester + H2O enantioselective reaction Bacillus cereus (S)-flurbiprofen ethyl ester + (R)-flurbiprofen + ethanol - ? 390461 3.1.1.3 ethyl 2-arylpropanoate + H2O - Bacillus cereus ethanol + (R)-2-arylpropanoate - ? 391925 3.1.1.3 additional information substrate specificity, the enantioselective lipase hydrolyzes 4-nitrophenyl esters with acyl chain lengths between C4 and C12, and shows high enantioselectivity toward the R-isomer of ethyl 2-arylpropanoate, overview Bacillus cereus ? - ? 89 3.1.1.3 olive oil + H2O - Bacillus cereus ? - ? 364063 3.1.1.3 olive oil + H2O - Bacillus cereus C(7) ? - ? 364063 3.1.1.3 (R,S)-flurbiprofen ethyl ester + H2O enantioselective reaction Bacillus cereus C71 (S)-flurbiprofen ethyl ester + (R)-flurbiprofen + ethanol - ? 390461 3.1.1.3 ethyl 2-arylpropanoate + H2O - Bacillus cereus C71 ethanol + (R)-2-arylpropanoate - ? 391925 3.1.1.3 additional information substrate specificity, the enantioselective lipase hydrolyzes 4-nitrophenyl esters with acyl chain lengths between C4 and C12, and shows high enantioselectivity toward the R-isomer of ethyl 2-arylpropanoate, overview Bacillus cereus C71 ? - ? 89 3.1.1.3 4-nitrophenyl palmitate + H2O - Bacillus licheniformis 4-nitrophenol + palmitate - ? 363890 3.1.1.3 glyceryl trioleate + 3 H2O 14% activity compared to glyceryl tributyrate Bacillus licheniformis glycerol + 3 oleate - ? 401074 3.1.1.3 sardine oil + H2O the lipase is used for hydrolysis of triacylglycerol in sardine oil to enrich DELTA5-polyunsaturated fatty acids namely, arachidonic acid and eicosapentaenoic acid Bacillus licheniformis ? - ? 401876 3.1.1.3 tributyrin + 3 H2O 100% activity Bacillus licheniformis glycerol + 3 butyrate - ? 402043 3.1.1.3 trilaurin + 3 H2O 66% activity compared to glyceryl tributyrate Bacillus licheniformis glycerol + 3 laurate - ? 402045 3.1.1.3 triolein + H2O - Bacillus licheniformis 1,2-diolein + oleate - ? 393714 3.1.1.3 tripalmitin + 3 H2O 42% activity compared to glyceryl tributyrate Bacillus licheniformis glycerol + 3 palmitate - ? 402051 3.1.1.3 tristearin + 3 H2O 31% activity compared to glyceryl tributyrate Bacillus licheniformis glycerol + 3 stearate - ? 402054 3.1.1.3 4-nitrophenyl palmitate + H2O - Bacillus licheniformis MTCC 6824 4-nitrophenol + palmitate - ? 363890 3.1.1.3 glyceryl trioleate + 3 H2O 14% activity compared to glyceryl tributyrate Bacillus licheniformis MTCC 6824 glycerol + 3 oleate - ? 401074 3.1.1.3 sardine oil + H2O the lipase is used for hydrolysis of triacylglycerol in sardine oil to enrich DELTA5-polyunsaturated fatty acids namely, arachidonic acid and eicosapentaenoic acid Bacillus licheniformis MTCC 6824 ? - ? 401876 3.1.1.3 tributyrin + 3 H2O 100% activity Bacillus licheniformis MTCC 6824 glycerol + 3 butyrate - ? 402043 3.1.1.3 trilaurin + 3 H2O 66% activity compared to glyceryl tributyrate Bacillus licheniformis MTCC 6824 glycerol + 3 laurate - ? 402045 3.1.1.3 triolein + H2O - Bacillus licheniformis MTCC 6824 1,2-diolein + oleate - ? 393714 3.1.1.3 4-nitrophenyl butyrate + H2O the enzyme shows a typical interfacial activation mechanism towards the substrate Bacillus pumilus 4-nitrophenol + butyrate - ? 115376 3.1.1.3 additional information an emulsion containing olive oil and gum arabic is used as substrate, enzyme shows a preference for short-chain triacylglycerides Bacillus pumilus ? - ? 89 3.1.1.3 tributyrin + H2O - Bacillus pumilus dibutyrin + butyrate - ? 363949 3.1.1.3 triolein + H2O low activity Bacillus pumilus diolein + oleate - ? 363933 3.1.1.3 tripropionin + H2O the enzyme shows a typical interfacial activation mechanism towarsd the substrate Bacillus pumilus dipropionin + propionate - ? 364079 3.1.1.3 4-nitrophenyl butyrate + H2O the enzyme shows a typical interfacial activation mechanism towards the substrate Bacillus pumilus B26 4-nitrophenol + butyrate - ? 115376 3.1.1.3 additional information an emulsion containing olive oil and gum arabic is used as substrate, enzyme shows a preference for short-chain triacylglycerides Bacillus pumilus B26 ? - ? 89 3.1.1.3 tributyrin + H2O - Bacillus pumilus B26 dibutyrin + butyrate - ? 363949 3.1.1.3 triolein + H2O low activity Bacillus pumilus B26 diolein + oleate - ? 363933 3.1.1.3 tripropionin + H2O the enzyme shows a typical interfacial activation mechanism towarsd the substrate Bacillus pumilus B26 dipropionin + propionate - ? 364079 3.1.1.3 (RS)-1-phenylethyl acetate + H2O enzyme hydrolyzes mainly the R-enantiomer, temperature-dependent, the enantioselectivity decreases with increasing temperature Bacillus sp. (in: Bacteria) (R)-1-phenylethanol + acetate + (R)-1-phenylethyl acetate - ? 364166 3.1.1.3 1-naphthyl octanoate + H2O - Bacillus sp. (in: Bacteria) 1-naphthol + octanoate - ? 416025 3.1.1.3 4-nitrophenyl acetate + H2O about 45% activity compared to 4-nitrophenyl butyrate Bacillus sp. (in: Bacteria) 4-nitrophenol + acetate - ? 12514 3.1.1.3 4-nitrophenyl butyrate + H2O - Bacillus sp. (in: Bacteria) 4-nitrophenol + butyrate - ? 115376 3.1.1.3 4-nitrophenyl butyrate + H2O low activity Bacillus sp. (in: Bacteria) 4-nitrophenol + butyrate - ? 115376 3.1.1.3 4-nitrophenyl butyrate + H2O best substrate for wild type enzyme Bacillus sp. (in: Bacteria) 4-nitrophenol + butyrate - ? 115376 3.1.1.3 4-nitrophenyl caprate + H2O best substrate Bacillus sp. (in: Bacteria) 4-nitrophenol + caprate - ? 364083 3.1.1.3 4-nitrophenyl caprylate + H2O best substrate Bacillus sp. (in: Bacteria) 4-nitrophenol + caprylate - ? 363889 3.1.1.3 4-nitrophenyl caprylate + H2O at 30°C, the enzyme prefers C8 synthetic substrate (4-nitrophenyl caprylate)to shorter or longer substrates Bacillus sp. (in: Bacteria) 4-nitrophenol + caprylate - ? 363889 3.1.1.3 4-nitrophenyl decanoate + H2O about 100% activity compared to 4-nitrophenyl butyrate Bacillus sp. (in: Bacteria) 4-nitrophenol + decanoate - ? 360778 3.1.1.3 4-nitrophenyl hexanoate + H2O - Bacillus sp. (in: Bacteria) 4-nitrophenol + hexanoate - ? 115379 3.1.1.3 4-nitrophenyl laurate + H2O - Bacillus sp. (in: Bacteria) 4-nitrophenol + laurate - ? 363891 3.1.1.3 4-nitrophenyl laurate + H2O best substrate Bacillus sp. (in: Bacteria) 4-nitrophenol + laurate - ? 363891 3.1.1.3 4-nitrophenyl laurate + H2O about 80% activity compared to 4-nitrophenyl butyrate Bacillus sp. (in: Bacteria) 4-nitrophenol + laurate - ? 363891 3.1.1.3 4-nitrophenyl myristate + H2O - Bacillus sp. (in: Bacteria) 4-nitrophenol + myristate - ? 364082 3.1.1.3 4-nitrophenyl myristate + H2O about 30% activity compared to 4-nitrophenyl butyrate Bacillus sp. (in: Bacteria) 4-nitrophenol + myristate - ? 364082 3.1.1.3 4-nitrophenyl palmitate + H2O - Bacillus sp. (in: Bacteria) 4-nitrophenol + palmitate - ? 363890 3.1.1.3 4-nitrophenyl palmitate + H2O low activity Bacillus sp. (in: Bacteria) 4-nitrophenol + palmitate - ? 363890 3.1.1.3 4-nitrophenyl palmitate + H2O about 30% activity compared to 4-nitrophenyl butyrate Bacillus sp. (in: Bacteria) 4-nitrophenol + palmitate - ? 363890 3.1.1.3 4-nitrophenyl stearate + H2O - Bacillus sp. (in: Bacteria) 4-nitrophenol + stearate - ? 364135 3.1.1.3 4-nitrophenyl stearate + H2O about 15% activity compared to 4-nitrophenyl butyrate Bacillus sp. (in: Bacteria) 4-nitrophenol + stearate - ? 364135 3.1.1.3 methyl(RS)-2-methyldecanoate + H2O enzyme hydrolyzes mainly the R-enantiomer, temperature-dependent, the enantioselectivity decreases with increasing temperature Bacillus sp. (in: Bacteria) (R)-2-methyldecanoic acid + methanol - ? 364167 3.1.1.3 additional information fatty acid substrate specificity is broad with little preference for C12 and C4 Bacillus sp. (in: Bacteria) ? - ? 89 3.1.1.3 additional information LipA has a preference for 4-nitrophenyl esters with longer fatty acid chains Bacillus sp. (in: Bacteria) ? - ? 89 3.1.1.3 additional information strain A30-1, active with triglycerides of C16:0 to C22:0fatty acids and on natural fats and oils Bacillus sp. (in: Bacteria) ? - ? 89 3.1.1.3 triacylglycerol + H2O the strain HH-01 degrades 1.0% (v/v) edible oil in nutrient broth, almost completely within 10 days Bacillus sp. (in: Bacteria) diacylglycerol + a carboxylate - ? 360693 3.1.1.3 tributyrin + H2O - Bacillus sp. (in: Bacteria) dibutyrin + butyrate - ? 363949 3.1.1.3 tributyrin + H2O - Bacillus sp. (in: Bacteria) dibutyrin + butyric acid - ? 364199 3.1.1.3 tricaprin + H2O - Bacillus sp. (in: Bacteria) dicaprin + capric acid - ? 364201 3.1.1.3 tricaproin + H2O - Bacillus sp. (in: Bacteria) dicaproin + caproic acid - ? 364200 3.1.1.3 tricaprylin + H2O - Bacillus sp. (in: Bacteria) dicaprylin + caprylic acid - ? 364202 3.1.1.3 trilaurin + H2O - Bacillus sp. (in: Bacteria) dilaurin + lauric acid - ? 364203 3.1.1.3 trilinolein + H2O the enzyme shows preferential hydrolysis of the 1(3)-position ester bond in trilinolein Bacillus sp. (in: Bacteria) dilinolein + linoleate - ? 364139 3.1.1.3 triolein + H2O - Bacillus sp. (in: Bacteria) diolein + oleate - ? 363933 3.1.1.3 triolein + H2O hydrolysis at all positions, strain J33 Bacillus sp. (in: Bacteria) diolein + oleate - ? 363933 3.1.1.3 triolein + H2O - Bacillus sp. (in: Bacteria) diolein + oleic acid - ? 364170 3.1.1.3 tripalmitin + H2O - Bacillus sp. (in: Bacteria) dipalmitin + palmitic acid - ? 364204 3.1.1.3 4-nitrophenyl butyrate + H2O - Bacillus sp. (in: Bacteria) HH-01 4-nitrophenol + butyrate - ? 115376 3.1.1.3 4-nitrophenyl caprylate + H2O at 30°C, the enzyme prefers C8 synthetic substrate (4-nitrophenyl caprylate)to shorter or longer substrates Bacillus sp. (in: Bacteria) HH-01 4-nitrophenol + caprylate - ? 363889 3.1.1.3 4-nitrophenyl laurate + H2O - Bacillus sp. (in: Bacteria) HH-01 4-nitrophenol + laurate - ? 363891 3.1.1.3 4-nitrophenyl palmitate + H2O - Bacillus sp. (in: Bacteria) HH-01 4-nitrophenol + palmitate - ? 363890 3.1.1.3 triacylglycerol + H2O the strain HH-01 degrades 1.0% (v/v) edible oil in nutrient broth, almost completely within 10 days Bacillus sp. (in: Bacteria) HH-01 diacylglycerol + a carboxylate - ? 360693 3.1.1.3 4-nitrophenyl laurate + H2O - Bacillus sp. (in: Bacteria) J33 4-nitrophenol + laurate - ? 363891 3.1.1.3 additional information strain A30-1, active with triglycerides of C16:0 to C22:0fatty acids and on natural fats and oils Bacillus sp. (in: Bacteria) J33 ? - ? 89 3.1.1.3 additional information fatty acid substrate specificity is broad with little preference for C12 and C4 Bacillus sp. (in: Bacteria) J33 ? - ? 89 3.1.1.3 tributyrin + H2O - Bacillus sp. (in: Bacteria) J33 dibutyrin + butyrate - ? 363949 3.1.1.3 tributyrin + H2O - Bacillus sp. (in: Bacteria) J33 dibutyrin + butyric acid - ? 364199 3.1.1.3 tricaproin + H2O - Bacillus sp. (in: Bacteria) J33 dicaproin + caproic acid - ? 364200 3.1.1.3 triolein + H2O hydrolysis at all positions, strain J33 Bacillus sp. (in: Bacteria) J33 diolein + oleate - ? 363933 3.1.1.3 triolein + H2O - Bacillus sp. (in: Bacteria) J33 diolein + oleic acid - ? 364170 3.1.1.3 4-nitrophenyl butyrate + H2O - Bacillus sp. (in: Bacteria) RSJ-1 4-nitrophenol + butyrate - ? 115376 3.1.1.3 triolein + H2O - Bacillus sp. (in: Bacteria) RSJ-1 diolein + oleate - ? 363933 3.1.1.3 4-nitrophenyl butyrate + H2O low activity Bacillus sp. (in: Bacteria) Tp10A.1 4-nitrophenol + butyrate - ? 115376 3.1.1.3 4-nitrophenyl caprylate + H2O best substrate Bacillus sp. (in: Bacteria) Tp10A.1 4-nitrophenol + caprylate - ? 363889 3.1.1.3 4-nitrophenyl hexanoate + H2O - Bacillus sp. (in: Bacteria) Tp10A.1 4-nitrophenol + hexanoate - ? 115379 3.1.1.3 4-nitrophenyl palmitate + H2O low activity Bacillus sp. (in: Bacteria) Tp10A.1 4-nitrophenol + palmitate - ? 363890 3.1.1.3 additional information LipA has a preference for 4-nitrophenyl esters with longer fatty acid chains Bacillus sp. (in: Bacteria) Tp10A.1 ? - ? 89 3.1.1.3 1,2-dilauryl-rac-glycero-3-glutaric acid resorufinester + H2O - Bacillus subtilis ? - ? 380828 3.1.1.3 4-nitrophenol butyrate + H2O - Bacillus subtilis 4-nitrophenol + butyrate - ? 364198 3.1.1.3 4-nitrophenyl laurate + H2O - Bacillus subtilis 4-nitrophenol + laurate - ? 363891 3.1.1.3 4-nitrophenyl palmitate + H2O - Bacillus subtilis 4-nitrophenol + palmitate - ? 363890 3.1.1.3 long-chain triacylglycerol + H2O - Bacillus subtilis diacylglycerol + a carboxylate - ? 392493 3.1.1.3 additional information enzyme does not possess protease activity and does not hydrolyze peptide bonds, no activity with N-benzoyl-L-tyrosine 4-nitroanilide Bacillus subtilis ? - ? 89 3.1.1.3 additional information substrate specificity of the extracellular enzyme, the enzyme shows low activity Bacillus subtilis ? - ? 89 3.1.1.3 tributyrin + H2O - Bacillus subtilis dibutyrin + butyrate - ? 363949 3.1.1.3 triolein + H2O - Bacillus subtilis diolein + oleate - ? 363933 3.1.1.3 4-nitrophenol butyrate + H2O - Bacillus subtilis 168 BsL 4-nitrophenol + butyrate - ? 364198 3.1.1.3 additional information enzyme does not possess protease activity and does not hydrolyze peptide bonds, no activity with N-benzoyl-L-tyrosine 4-nitroanilide Bacillus subtilis 168 BsL ? - ? 89 3.1.1.3 tributyrin + H2O - Bacillus subtilis 168 BsL dibutyrin + butyrate - ? 363949 3.1.1.3 4-nitrophenyl laurate + H2O - Bacillus subtilis FH5 4-nitrophenol + laurate - ? 363891 3.1.1.3 long-chain triacylglycerol + H2O - Bacillus subtilis FH5 diacylglycerol + a carboxylate - ? 392493 3.1.1.3 4-nitrophenyl caprate + H2O - Blastobotrys adeninivorans 4-nitrophenol + caprate - ? 364083 3.1.1.3 4-nitrophenyl caprylate + H2O - Blastobotrys adeninivorans 4-nitrophenol + caprylate - ? 363889 3.1.1.3 additional information the enzyme prefers substrates containing middle-size fatty acids Blastobotrys adeninivorans ? - ? 89 3.1.1.3 tricaprylin + H2O best substrate Blastobotrys adeninivorans dicaprylin + caprylate - ? 364128 3.1.1.3 1,2-didecanoyl-1-thioglycerol + H2O - Bos taurus ? - ? 390489 3.1.1.3 1-stearoyl-2-arachidonoyl-sn-glycerol + H2O - Bos taurus ? - ? 390557 3.1.1.3 additional information the enzyme is involved in basal lipolysis of storage triacylglycerides, and hormonally as well as developmentally regulated, physiological functions, role in apoB-containing lipoprotein assembly in the endoplasmic reticulum, overview Bos taurus ? - ? 89 3.1.1.3 additional information diacylglycerol lipase may regulate protein kinase C activity and 2-arachidonoyl-sn-glycerol levels by rapidly altering the intracellular concentration of diacylglycerols Bos taurus ? - ? 89 3.1.1.3 additional information 1,2-diacyl-sn-glycerols are the preferred substrates over 1,3-diacyl-sn-glycerols. The enzyme hydrolyzes stearate in preference to palmitate from the sn-1-position of 1,2-diacyl-sn-glycerols. 1-O-alkyl-2-acyl-sn-glycerols are not a substrate for the enzyme Bos taurus ? - ? 89 3.1.1.3 trioleoylglycerol + H2O - Bos taurus oleic acid + ? - ? 64389 3.1.1.3 additional information the enzyme hydrolyzes triacylglycerols to fatty acids and glycerol, the fatty acids from the sn-1 or 3-positions are hydrolyzed first to yield 1,2(2,3)-diacylglycerols Brassica napus ? - ? 89 3.1.1.3 triarachidin + H2O - Brassica napus arachidonic acid + ? - ? 64404 3.1.1.3 trieicosenoin + H2O - Brassica napus ? - ? 64405 3.1.1.3 trierucin + H2O - Brassica napus eicosanoic acid + ? - ? 64406 3.1.1.3 trilaurin + H2O - Brassica napus lauric acid + ? - ? 64396 3.1.1.3 trilinolein + H2O - Brassica napus linolic acid + ? - ? 64407 3.1.1.3 trilinolenein + H2O - Brassica napus linolenic acid + ? - ? 64408 3.1.1.3 trimyristin + H2O - Brassica napus myristic acid + ? - ? 64401 3.1.1.3 trioleoylglycerol + H2O - Brassica napus oleic acid + ? - ? 64389 3.1.1.3 tripalmitin + H2O - Brassica napus palmitic acid + ? - ? 64402 3.1.1.3 tristearin + H2O - Brassica napus stearic acid + ? - ? 64403 3.1.1.3 (R,S)-[4-[4a,6b(E)]]-6-[4,4-bis(4-fluorophenyl)-3-(1-methyl-1H-tetrazol-5-yl)-1,3-butadienyl]-tetrahydro-4-hydroxy-2H-pyran-2-one + isopropenyl acetate - Burkholderia cepacia (R)-(+)-[4-[4a,6b(E)]]-6-[4,4-bis(4-fluorophenyl)-3-(1-methyl-1H-tetrazol-5-yl)-1,3-butadienyl]-tetrahydro-4-hydroxy-2H-pyran-2-one + (S)-(-)-[4-[4a,6b(E)]]-6-[4,4-bis(4-fluorophenyl)-3-(1-methyl-1H-tetrazol-5-yl)-1,3-butadienyl]-tetrahydro-4-acetyloxy-2H-pyran-2-one + prop-1-en-2-ol - ? 375474 3.1.1.3 1,2-dilauryl-rac-glycero-3-glutaric acid resorufinester + H2O - Burkholderia cepacia ? - ? 380828 3.1.1.3 4-nitrophenyl palmitate + H2O - Burkholderia cepacia 4-nitrophenol + palmitate - ? 363890 3.1.1.3 4-nitrophenyl palmitate + H2O in n-heptane Burkholderia cepacia 4-nitrophenol + palmitate - ? 363890 3.1.1.3 cis-3-(acetyloxy)-4-phenyl-2-azetidinone + H2O - Burkholderia cepacia (3R,4S)-cis-3-(acetyloxy)-4-phenyl-2-azetidinone + (3S,4R)-cis-3-hydroxy-4-phenyl-2-azetidinone + acetate - ? 376496 3.1.1.3 high linoleic sunflower oil + H2O - Burkholderia cepacia ? - ? 401143 3.1.1.3 high oleic sunflower oil + H2O - Burkholderia cepacia ? - ? 401146 3.1.1.3 additional information substrate specificity of the extracellular enzyme Burkholderia cepacia ? - ? 89 3.1.1.3 additional information substrate specificity for saturated fatty acids from C6 to C12 and unsaturated long-chain fatty acids. Monoglycerides are hydrolyzed very slowly Burkholderia cepacia ? - ? 89 3.1.1.3 olive oil + H2O - Burkholderia cepacia ? - ? 364063 3.1.1.3 tributyrin + H2O - Burkholderia cepacia dibutyrin + butyrate - ? 363949 3.1.1.3 triolein + H2O - Burkholderia cepacia diolein + oleate - ? 363933 3.1.1.3 vinyl butyrate + H2O - Burkholderia cepacia ethenol + butyrate - ? 402236 3.1.1.3 additional information substrate specificity for saturated fatty acids from C6 to C12 and unsaturated long-chain fatty acids. Monoglycerides are hydrolyzed very slowly Burkholderia cepacia ATCC 21808 ? - ? 89 3.1.1.3 triolein + H2O - Burkholderia cepacia ATCC 21808 diolein + oleate - ? 363933 3.1.1.3 additional information catalytic triad of the active center consists of the residues Ser87, Asp263 and His285. These residues are not exposed to the solvent, but a narrow channel connects them with the molecular surface Burkholderia glumae ? - ? 89 3.1.1.3 4-nitrophenyl palmitate + H2O - Burkholderia multivorans 4-nitrophenol + palmitate - ? 363890 3.1.1.3 4-nitrophenyl palmitate + H2O - Burkholderia multivorans RG2 4-nitrophenol + palmitate - ? 363890 3.1.1.3 (R,S)-1-phenylethylamine + ethyl methoxyacetate - Burkholderia plantarii (S)-1-phenylethylamine + 2-methoxy-N-[(1R)-1-phenylethyl]acetamide + ethanol - ? 375468 3.1.1.3 4-nitrophenyl butyrate + H2O - Burkholderia sp. 4-nitrophenol + butyrate - ? 115376 3.1.1.3 methyl (R)-mandelate + H2O - Burkholderia sp. (R)-mandelic acid + methanol - ? 399210 3.1.1.3 tert-butyl octanoate + H2O preferred substrate, high activity with Burkholderia sp. tert-butanol + octanoate - ? 364093 3.1.1.3 tributyrin + H2O - Burkholderia sp. dibutyrin + butyrate - ? 363949 3.1.1.3 4-nitrophenyl butyrate + H2O - Burkholderia sp. GXU56 4-nitrophenol + butyrate - ? 115376 3.1.1.3 methyl (R)-mandelate + H2O - Burkholderia sp. GXU56 (R)-mandelic acid + methanol - ? 399210 3.1.1.3 dicaprin + H2O hydrolyses more efficiently the 2,3-sn-dicaprin isomer than the 1,2-sn-dicaprin and the 1,3-sndicaprin monolayers Camelus dromedarius caprin + caprate - ? 381799 3.1.1.3 olive oil + H2O - Camelus dromedarius ? - ? 364063 3.1.1.3 tributyrin + H2O - Camelus dromedarius dibutyrin + butyrate - ? 363949 3.1.1.3 trioctanoin + H2O - Camelus dromedarius dioctanoin + octanoate - ? 393712 3.1.1.3 triolein + H2O - Camelus dromedarius diolein + oleate - ? 363933 3.1.1.3 4-nitrophenyl butyrate + H2O - Candida albicans 4-nitrophenol + butyrate - ? 115376 3.1.1.3 4-nitrophenyl caprylate + H2O highest activity of mutant enzyme S154L/S293L Candida albicans 4-nitrophenol + caprylate - ? 363889 3.1.1.3 4-nitrophenyl laurate + H2O lowest activity of mutant enzyme S154L/S293L Candida albicans 4-nitrophenol + laurate - ? 363891 3.1.1.3 4-nitrophenyl palmitate + H2O - Candida albicans 4-nitrophenol + palmitate - ? 363890 3.1.1.3 additional information the enzyme also uses olive oil and safflower oil as substrates Candida albicans ? - ? 89 3.1.1.3 4-nitrophenyl butyrate + H2O - Candida albicans ATCC 10231 4-nitrophenol + butyrate - ? 115376 3.1.1.3 4-nitrophenyl caprylate + H2O highest activity of mutant enzyme S154L/S293L Candida albicans ATCC 10231 4-nitrophenol + caprylate - ? 363889 3.1.1.3 4-nitrophenyl laurate + H2O lowest activity of mutant enzyme S154L/S293L Candida albicans ATCC 10231 4-nitrophenol + laurate - ? 363891 3.1.1.3 4-nitrophenyl palmitate + H2O - Candida albicans ATCC 10231 4-nitrophenol + palmitate - ? 363890 3.1.1.3 additional information the enzyme also uses olive oil and safflower oil as substrates Candida albicans ATCC 10231 ? - ? 89 3.1.1.3 (R/S)-ibuprofen methoxyethyl ester + H2O - Candida cyclindraceae (R)-ibuprofen methoxyethyl ester + (S)-ibuprofen + 2-methoxyethanol - ? 375477 3.1.1.3 additional information the enzyme preferentially performs transfer reactions such as alcoholysis in presence of suitable nucleophiles other than water, and catalyzes the hydrolysis of water insoluble medium-chain triacylglyceride substrates Candida parapsilosis ? - ? 89 3.1.1.3 (R,S)-naproxen 2,2,2-trifluoroethyl thioester (R,S)-profen 2,2,2-trifluoroethyl thioester Carica papaya (S)-naproxen + 2,2,2-trifluorothioethanol + (R)-naproxen 2,2,2-trifluoroethyl thioester - ? 382401 3.1.1.3 fenoprofen + H2O (R,S)-profen 2,2,2-trifluoroethyl thioester Carica papaya ? - ? 381862 3.1.1.3 flurbiprofen + H2O (R,S)-profen 2,2,2-trifluoroethyl thioester Carica papaya ? - ? 381873 3.1.1.3 ibuprofen + H2O (R,S)-profen 2,2,2-trifluoroethyl thioester Carica papaya ? - ? 382049 3.1.1.3 ketoprofen + H2O (R,S)-profen 2,2,2-trifluoroethyl thioester Carica papaya ? - ? 382089 3.1.1.3 additional information temperature-dependent substrate specificity of isozymes with (R,S)-profen 2,2,2-trifluoroethyl thioesters, overview Carica papaya ? - ? 89 3.1.1.3 olive oil + H2O - Carica papaya ? - ? 364063 3.1.1.3 tributyrin + H2O - Carica papaya dibutyrin + butyrate - ? 363949 3.1.1.3 triolein + H2O olive oil in aqueous solution, enantioselective hydrolysis Carica papaya diolein + oleate - ? 363933 3.1.1.3 4-nitrophenyl acetate + H2O low activity Cephaloleia presignis 4-nitrophenol + acetate - ? 12514 3.1.1.3 4-nitrophenyl butyrate + H2O low activity Cephaloleia presignis 4-nitrophenol + butyrate - ? 115376 3.1.1.3 4-nitrophenyl laurate + H2O - Cephaloleia presignis 4-nitrophenol + laurate - ? 363891 3.1.1.3 4-nitrophenyl oleate + H2O - Cephaloleia presignis 4-nitrophenol + oleate - ? 364073 3.1.1.3 4-nitrophenyl palmitate + H2O high activity Cephaloleia presignis 4-nitrophenol + palmitate - ? 363890 3.1.1.3 4-nitrophenyl propionate + H2O - Cephaloleia presignis 4-nitrophenol + propionate - ? 115374 3.1.1.3 alpha-naphthyl acetate + H2O - Cephaloleia presignis alpha-naphthol + acetate - ? 36052 3.1.1.3 methyl acetate + H2O - Cephaloleia presignis methanol + acetate - ? 36055 3.1.1.3 methyl butyrate + H2O - Cephaloleia presignis methanol + butyrate - ? 115393 3.1.1.3 methyl laurate + H2O - Cephaloleia presignis methanol + laurate - ? 364077 3.1.1.3 methyl oleate + H2O high activity Cephaloleia presignis methanol + oleate - ? 364075 3.1.1.3 methyl palmitate + H2O high activity Cephaloleia presignis methanol + palmitate - ? 364076 3.1.1.3 methyl propionate + H2O - Cephaloleia presignis methanol + propionate - ? 115453 3.1.1.3 methyl stearate + H2O - Cephaloleia presignis methanol + stearate - ? 364078 3.1.1.3 additional information olive oil is utilized as substrate with high activity, the enzyme hydrolyzes long-chain fatty acids rather than short-chain fatty acids of 4-nitrophenyl esters and methyl esters, and it hydrolyzes short-chain fatty acids rather than short-shain fatty acids of triglycerides Cephaloleia presignis ? - ? 89 3.1.1.3 tributyrin + H2O best substrate Cephaloleia presignis dibutyrin + butyrate - ? 363949 3.1.1.3 trilaurin + H2O high activity Cephaloleia presignis dilaurin + laurate - ? 364081 3.1.1.3 triolein + H2O - Cephaloleia presignis diolein + oleate - ? 363933 3.1.1.3 tripalmitin + H2O - Cephaloleia presignis dipalmitin + palmitate - ? 364080 3.1.1.3 tripropionin + H2O - Cephaloleia presignis dipropionin + propionate - ? 364079 3.1.1.3 diacylglycerol-N,N,N-trimethylhomoserine + H2O - Chlamydomonas reinhardtii ? - ? 430113 3.1.1.3 digalactosyldiacylglycerol + H2O - Chlamydomonas reinhardtii ? - ? 369683 3.1.1.3 monogalactosyldiacylglycerol + H2O - Chlamydomonas reinhardtii ? - ? 369682 3.1.1.3 olive oil + H2O - Chlamydomonas reinhardtii ? - ? 364063 3.1.1.3 phosphatidylethanolamine + H2O - Chlamydomonas reinhardtii ? - ? 64438 3.1.1.3 phosphatidylglycerol + H2O - Chlamydomonas reinhardtii ? - ? 73203 3.1.1.3 triacylglycerol + H2O - Chlamydomonas reinhardtii diacylglycerol + a carboxylate - ? 360693 3.1.1.3 monogalactosyldiacylglycerol + H2O - Chlamydomonas reinhardtii dw15.1 ? - ? 369682 3.1.1.3 olive oil + H2O - Chlamydomonas reinhardtii dw15.1 ? - ? 364063 3.1.1.3 phosphatidylethanolamine + H2O - Chlamydomonas reinhardtii dw15.1 ? - ? 64438 3.1.1.3 phosphatidylglycerol + H2O - Chlamydomonas reinhardtii dw15.1 ? - ? 73203 3.1.1.3 triacylglycerol + H2O - Chlamydomonas reinhardtii dw15.1 diacylglycerol + a carboxylate - ? 360693 3.1.1.3 additional information enzyme does not possess protease activity and does not hydrolyze peptide bonds, no activity with N-benzoyl-L-tyrosine 4-nitroanilide Chromobacterium viscosum ? - ? 89 3.1.1.3 tributyrin + H2O - Chromobacterium viscosum dibutyrin + butyrate - ? 363949 3.1.1.3 additional information with p-nitrophenyl esters of different fatty acids as substrates enzyme activity is highest when the acyl chain is short e.g. C2 Clostridium tetanomorphum ? - ? 89 3.1.1.3 tributyrin + H2O - Clostridium tetanomorphum dibutyrin + butyrate - ? 363949 3.1.1.3 tricaprin + H2O - Clostridium tetanomorphum dicaprin + caprate - ? 364183 3.1.1.3 tricaproin + H2O - Clostridium tetanomorphum dicaproin + caproate - ? 364147 3.1.1.3 tricaprylin + H2O - Clostridium tetanomorphum dicaprylin + caprylate - ? 364128 3.1.1.3 trilaurin + H2O - Clostridium tetanomorphum dilaurin + laurate - ? 364081 3.1.1.3 triolein + H2O - Clostridium tetanomorphum diolein + oleate - ? 363933 3.1.1.3 tripalmitin + H2O - Clostridium tetanomorphum dipalmitin + palmitate - ? 364080 3.1.1.3 additional information with p-nitrophenyl esters of different fatty acids as substrates enzyme activity is highest when the acyl chain is short e.g. C2 Clostridium tetanomorphum NCTC 543 ? - ? 89 3.1.1.3 tributyrin + H2O - Clostridium tetanomorphum NCTC 543 dibutyrin + butyrate - ? 363949 3.1.1.3 trilaurin + H2O - Clostridium tetanomorphum NCTC 543 dilaurin + laurate - ? 364081 3.1.1.3 triolein + H2O - Clostridium tetanomorphum NCTC 543 diolein + oleate - ? 363933 3.1.1.3 tripalmitin + H2O - Clostridium tetanomorphum NCTC 543 dipalmitin + palmitate - ? 364080 3.1.1.3 4-nitrophenyl palmitate + H2O - Cupriavidus necator 4-nitrophenol + palmitate - ? 363890 3.1.1.3 additional information LipA is a non-specific lipase able to act at random for the complete breakdown of triacylglycerols Cupriavidus necator ? - ? 89 3.1.1.3 4-nitrophenyl palmitate + H2O - Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 4-nitrophenol + palmitate - ? 363890 3.1.1.3 additional information LipA is a non-specific lipase able to act at random for the complete breakdown of triacylglycerols Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 ? - ? 89 3.1.1.3 1,2-O-dilauryl-rac-glycero-3-glutaric resorufin ester + H2O commercial chromogenic lipase substrate Diutina rugosa ? - ? 380832 3.1.1.3 2-(4-chlorophenoxy)acetic acid ethyl ester + H2O the enantioselectivity of the hydrolysis of the 2-substituted-aryloxyacetic ester is increased by addition of isopropanol or DMSO as co-solvents Diutina rugosa ? - ? 390604 3.1.1.3 2-n-butyl-2-(4-chlorophenoxy)acetic acid ethyl ester + H2O the enantioselectivity of the hydrolysis of the 2-substituted-aryloxyacetic ester is increased by addition of isopropanol or DMSO as co-solvents Diutina rugosa ? - ? 390654 3.1.1.3 2-phenyl-2-(4-chlorophenoxy)acetic acid ethyl ester + H2O the enantioselectivity of the hydrolysis of the 2-substituted-aryloxyacetic ester is increased by addition of isopropanol or DMSO as co-solvents Diutina rugosa ? - ? 390665 3.1.1.3 4-nitrophenyl butyrate + H2O - Diutina rugosa 4-nitrophenol + butyrate - ? 115376 3.1.1.3 4-nitrophenyl caproate + H2O good substrate Diutina rugosa 4-nitrophenol + caproate - ? 363887 3.1.1.3 4-nitrophenyl caprylate + H2O - Diutina rugosa 4-nitrophenol + caprylate - ? 363889 3.1.1.3 4-nitrophenyl caprylate + H2O preferred substrate of lipase A Diutina rugosa 4-nitrophenol + caprylate - ? 363889 3.1.1.3 4-nitrophenyl decanoate + H2O good substrate Diutina rugosa 4-nitrophenol + decanoate - ? 360778 3.1.1.3 4-nitrophenyl laurate + H2O best substrate Diutina rugosa 4-nitrophenol + laurate - ? 363891 3.1.1.3 4-nitrophenyl laurate + H2O preferred substrate of lipase B Diutina rugosa 4-nitrophenol + laurate - ? 363891 3.1.1.3 4-nitrophenyl myristate + H2O - Diutina rugosa 4-nitrophenol + myristate - ? 364082 3.1.1.3 4-nitrophenyl palmitate + H2O - Diutina rugosa 4-nitrophenol + palmitate - ? 363890 3.1.1.3 4-nitrophenyl palmitate + H2O lower activity Diutina rugosa 4-nitrophenol + palmitate - ? 363890 3.1.1.3 canola oil + H2O - Diutina rugosa ? - ? 400605 3.1.1.3 dextran T40 + vinyl acetate when vinyl acetate is used as acyl donor, percent modification of DexT40 increases from 31.8% to 60.5% in the presence of enzyme Diutina rugosa ? - ? 416883 3.1.1.3 dextran T40 + vinyl acrylate acylation with vinyl crotonate to DexT40 in DMSO demonstrates modification of 74.3% with addition of enzyme, compared to 25.5% without biocatalyst Diutina rugosa ? - ? 416884 3.1.1.3 dextran T40 + vinyl crotonate acylation with vinyl crotonate to DexT40 in DMSO demonstrates modification of 25.0% with addition of enzyme, compared to 0% without biocatalyst Diutina rugosa ? - ? 416885 3.1.1.3 dextran T40 + vinyl decanoate native lipase, pH-adjusted lipase, pH-adjusted lipase co-lyophilized with 18-crown-6 ether, and the lipase stepwise addition reaction display 3%, 49%, 64% and 96% conversion, respectively Diutina rugosa ? - ? 416886 3.1.1.3 dextran T40 + vinyl laurate enzymatic modification of DexT40 causes notably high extent of modification between 40 and 50%, as compared to less than 3% without enzyme Diutina rugosa ? - ? 416887 3.1.1.3 dextran T40 + vinyl methacrylate acylation with vinyl crotonate to DexT40 in DMSO demonstrates modification of 51.2% with addition of enzyme, compared to 1.7% without biocatalyst Diutina rugosa ? - ? 416888 3.1.1.3 dextran T40 + vinyl pivalate - Diutina rugosa ? - ? 416889 3.1.1.3 dextran T40 + vinyl propionate the enzyme accelerates the reaction with vinyl propionate up to 58.6% compared to 8.2% without enzyme Diutina rugosa ? - ? 416890 3.1.1.3 glycerol + caprylate esterification Diutina rugosa ? - ? 401070 3.1.1.3 high linoleic sunflower oil + H2O - Diutina rugosa ? - ? 401143 3.1.1.3 high oleic sunflower oil + H2O - Diutina rugosa ? - ? 401146 3.1.1.3 additional information enzyme does not possess protease activity and does not hydrolyze peptide bonds, no activity with N-benzoyl-L-tyrosine 4-nitroanilide Diutina rugosa ? - ? 89 3.1.1.3 additional information enzyme regulation Diutina rugosa ? - ? 89 3.1.1.3 additional information substrate specificities of wild-type and mutant isozymes, possible reactions are hydrolysis, direct esterification, acidolysis, alcoholysis, ester-interchange, and glycerolysis Diutina rugosa ? - ? 89 3.1.1.3 additional information the enantioselectivity of the hydrolysis of three different 2-substituted-aryloxyacetic esters is increased by addition of isopropanol or DMSO Diutina rugosa ? - ? 89 3.1.1.3 additional information in the conversion of babassu oil into alkyl esters, butanol gives the highest conversion (79.35%). Babassu oil consists of 3.5% caprylic, 4.5% capric, 44.7% lauric, 17.5% myristic, 9.7% palmitic, 3.1% stearic, 15.2% oleic, and 1.8% linoleic acid Diutina rugosa ? - ? 89 3.1.1.3 additional information lipase AY has the loose regioselectivity toward 2-OH and 3-OH in the glucose unit of dextran Diutina rugosa ? - ? 89 3.1.1.3 additional information the enzyme uses olive oil as substrate Diutina rugosa ? - ? 89 3.1.1.3 olive oil + H2O - Diutina rugosa ? - ? 364063 3.1.1.3 rac 2-(4-chlorophenoxy)propanoic acid + n-butanol esterification reaction, in n-heptane Diutina rugosa ? - ? 364088 3.1.1.3 racemic butyl 2-(4-ethylphenoxy)propionate + H2O modified enzyme shows reduced activity and 15fold increased enantioselectivity Diutina rugosa 2-(4-ethylphenoxy)propionate + butanol - ? 364091 3.1.1.3 soybean oil + H2O - Diutina rugosa ? - ? 364064 3.1.1.3 sulcatol + fatty acid esterification reaction, in toluene Diutina rugosa ? - ? 364089 3.1.1.3 triacetin + H2O - Diutina rugosa diacetin + acetic acid - ? 364154 3.1.1.3 tributyrin + H2O - Diutina rugosa dibutyrin + butyrate - ? 363949 3.1.1.3 tributyrin + H2O - Diutina rugosa dibutyrin + butyrate - r 363949 3.1.1.3 tricaprylin + H2O - Diutina rugosa dicaprylin + caprylate - ? 364128 3.1.1.3 triolein + H2O - Diutina rugosa diolein + oleate - r 363933 3.1.1.3 L-alpha-phosphatidylcholine + H2O - Equus caballus ? - ? 401243 3.1.1.3 additional information PLRP and pancreatic lipase differ in enzymatic properties such as substrate specificity, sensitivity to inhibition by bile salts and colipase dependence Equus caballus ? - ? 89 3.1.1.3 olive oil + H2O - Equus caballus ? - ? 364063 3.1.1.3 triacylglycerol + H2O - Equus caballus diacylglycerol + a carboxylate - ? 360693 3.1.1.3 tributyrin + H2O - Equus caballus ? - ? 64455 3.1.1.3 tributyrin + H2O - Equus caballus dibutyrin + butyrate - ? 363949 3.1.1.3 4-nitrophenyl acetate + H2O - Fagopyrum esculentum 4-nitrophenol + acetate - ? 12514 3.1.1.3 4-nitrophenyl butyrate + H2O - Fagopyrum esculentum 4-nitrophenol + butyrate - ? 115376 3.1.1.3 4-nitrophenyl caproate + H2O - Fagopyrum esculentum 4-nitrophenol + caproate - ? 363887 3.1.1.3 4-nitrophenyl laurate + H2O best substrate Fagopyrum esculentum 4-nitrophenol + laurate - ? 363891 3.1.1.3 olein + H2O low activity with isozyme LIP II, residual activity with isozyme LIP I Fagopyrum esculentum glycerol + oleate - ? 382442 3.1.1.3 palmitin + H2O low activity with isozyme LIP II, residual activity with isozyme LIP I Fagopyrum esculentum glycerol + palmitate - ? 382520 3.1.1.3 triolein + H2O good substrate Fagopyrum esculentum diolein + oleate - ? 363933 3.1.1.3 tripalmitin + H2O low activity with isozyme LIP II, residual activity with isozyme LIP I Fagopyrum esculentum dipalmitin + palmitate - ? 364080 3.1.1.3 4-nitrophenyl caproate + H2O - Fervidobacterium changbaicum 4-nitrophenol + caproate - ? 363887 3.1.1.3 tricaprylin + H2O - Fervidobacterium changbaicum dicaprylin + caprylate - ? 364128 3.1.1.3 4-nitrophenyl caproate + H2O - Fervidobacterium changbaicum CBS-1 4-nitrophenol + caproate - ? 363887 3.1.1.3 tricaprylin + H2O - Fervidobacterium changbaicum CBS-1 dicaprylin + caprylate - ? 364128 3.1.1.3 4-nitrophenyl acetate + H2O - Fusarium graminearum 4-nitrophenol + acetate - ? 12514 3.1.1.3 4-nitrophenyl butyrate + H2O - Fusarium graminearum 4-nitrophenol + butyrate - ? 115376 3.1.1.3 4-nitrophenyl caprate + H2O best substrate for isoforms FGL2 and FGL3 Fusarium graminearum 4-nitrophenol + caprate - ? 364083 3.1.1.3 4-nitrophenyl caproate + H2O - Fusarium graminearum 4-nitrophenol + caproate - ? 363887 3.1.1.3 4-nitrophenyl caprylate + H2O best substrate for isoform FGL5 Fusarium graminearum 4-nitrophenol + caprylate - ? 363889 3.1.1.3 4-nitrophenyl laurate + H2O best substrate for isoform FGL24 Fusarium graminearum 4-nitrophenol + laurate - ? 363891 3.1.1.3 4-nitrophenyl myristate + H2O best substrate for isoform FGL1 Fusarium graminearum 4-nitrophenol + myristate - ? 364082 3.1.1.3 4-nitrophenyl palmitate + H2O - Fusarium graminearum 4-nitrophenol + palmitate - ? 363890 3.1.1.3 4-nitrophenyl propionate + H2O - Fusarium graminearum 4-nitrophenol + propionate - ? 115374 3.1.1.3 4-nitrophenyl stearate + H2O - Fusarium graminearum 4-nitrophenol + stearate - ? 364135 3.1.1.3 additional information enzyme also utilizes water-soluble substrates, enzyme does not possess protease activity and does not hydrolyze peptide bonds, no activity with N-benzoyl-L-tyrosine 4-nitroanilide Fusarium solani ? - ? 89 3.1.1.3 tributyrin + H2O - Fusarium solani dibutyrin + butyrate - ? 363949 3.1.1.3 triolein + H2O - Fusarium solani diolein + oleate - ? 363933 3.1.1.3 additional information catalyzes the initial step in triglyceride hydrolysis Gallus gallus ? - ? 89 3.1.1.3 olive oil + H2O the enzyme can tolerate the accumulation of long-chain free fatty acids at the interface when olive oil emulsion is used as substrate in the absence of bile salts and colipase Gallus gallus ? - ? 364063 3.1.1.3 tributyrin + H2O - Gallus gallus dibutyrin + butyrate - ? 363949 3.1.1.3 tripropionin + H2O - Gallus gallus dipropionin + propionate - ? 364079 3.1.1.3 vinyl propionate + H2O - Gallus gallus ? - ? 393826 3.1.1.3 additional information the lipase hydrolyzes triacylglycerol to produce 1,2-diacylglycerol (with yield of 50.2%) and 2-monoacylglycerol (with yield of 14.0%) as main products and free fatty acids Geobacillus sp. ? - ? 89 3.1.1.3 triolein + H2O cleavage occurs only at the Sn-1 and Sn-3 positions Geobacillus sp. ? - ? 64454 3.1.1.3 additional information the lipase hydrolyzes triacylglycerol to produce 1,2-diacylglycerol (with yield of 50.2%) and 2-monoacylglycerol (with yield of 14.0%) as main products and free fatty acids Geobacillus sp. ARM ? - ? 89 3.1.1.3 triolein + H2O cleavage occurs only at the Sn-1 and Sn-3 positions Geobacillus sp. ARM ? - ? 64454 3.1.1.3 4-nitrophenyl acetate + H2O - Geobacillus stearothermophilus 4-nitrophenol + acetate - ? 12514 3.1.1.3 4-nitrophenyl caprylate + H2O - Geobacillus stearothermophilus 4-nitrophenol + caprylate - ? 363889 3.1.1.3 4-nitrophenyl caprylate + H2O L1, best substrate Geobacillus stearothermophilus 4-nitrophenol + caprylate - ? 363889 3.1.1.3 4-nitrophenyl palmitate + H2O best substrate Geobacillus stearothermophilus 4-nitrophenol + palmitate - ? 363890 3.1.1.3 DL-alpha-palmitin + H2O - Geobacillus stearothermophilus ? - ? 364181 3.1.1.3 DL-alpha-stearin + H2O - Geobacillus stearothermophilus ? - ? 364182 3.1.1.3 additional information substrate specificity, overview Geobacillus stearothermophilus ? - ? 89 3.1.1.3 additional information substrate is olive oil emulsion Geobacillus stearothermophilus ? - ? 89 3.1.1.3 additional information the enzyme also possesses esterase activity Geobacillus stearothermophilus ? - ? 89 3.1.1.3 olive oil + H2O emulsion Geobacillus stearothermophilus ? - ? 364063 3.1.1.3 triacetin + H2O - Geobacillus stearothermophilus diacetin + acetate - ? 364163 3.1.1.3 tributyrin + H2O - Geobacillus stearothermophilus dibutyrin + butyrate - ? 363949 3.1.1.3 tributyrin + H2O best substrate Geobacillus stearothermophilus dibutyrin + butyrate - ? 363949 3.1.1.3 tricaprin + H2O - Geobacillus stearothermophilus dicaprin + capriate - ? 364136 3.1.1.3 tricaprylin + H2O - Geobacillus stearothermophilus dicaprylin + caprylate - ? 364128 3.1.1.3 tricaprylin + H2O best substrate Geobacillus stearothermophilus dicaprylin + caprylate - ? 364128 3.1.1.3 trilaurin + H2O - Geobacillus stearothermophilus dilaurin + laurate - ? 364081 3.1.1.3 triolein + H2O - Geobacillus stearothermophilus diolein + oleate - ? 363933 3.1.1.3 tripalmitin + H2O - Geobacillus stearothermophilus dipalmitin + palmitate - ? 364080 3.1.1.3 tristearin + H2O - Geobacillus stearothermophilus distearin + stearate - ? 364134 3.1.1.3 Tween 80 + H2O i.e. poly(oxyethylene) sorbitan monooleate Geobacillus stearothermophilus ? - ? 364144 3.1.1.3 tributyrin + H2O - Geobacillus stearothermophilus AB-1 dibutyrin + butyrate - ? 363949 3.1.1.3 4-nitrophenyl caprylate + H2O L1, best substrate Geobacillus stearothermophilus L1 4-nitrophenol + caprylate - ? 363889 3.1.1.3 additional information substrate is olive oil emulsion Geobacillus stearothermophilus L1 ? - ? 89 3.1.1.3 tributyrin + H2O - Geobacillus stearothermophilus L1 dibutyrin + butyrate - ? 363949 3.1.1.3 4-nitrophenyl acetate + H2O - Geobacillus stearothermophilus MC 7 4-nitrophenol + acetate - ? 12514 3.1.1.3 4-nitrophenyl palmitate + H2O best substrate Geobacillus stearothermophilus MC 7 4-nitrophenol + palmitate - ? 363890 3.1.1.3 additional information the enzyme also possesses esterase activity Geobacillus stearothermophilus MC 7 ? - ? 89 3.1.1.3 tributyrin + H2O best substrate Geobacillus stearothermophilus MC 7 dibutyrin + butyrate - ? 363949 3.1.1.3 triolein + H2O - Geobacillus stearothermophilus MC 7 diolein + oleate - ? 363933 3.1.1.3 4-nitrophenyl caprylate + H2O - Geobacillus stearothermophilus P1 4-nitrophenol + caprylate - ? 363889 3.1.1.3 additional information substrate specificity, overview Geobacillus stearothermophilus P1 ? - ? 89 3.1.1.3 tricaprylin + H2O - Geobacillus stearothermophilus P1 dicaprylin + caprylate - ? 364128 3.1.1.3 tricaprylin + H2O best substrate Geobacillus stearothermophilus P1 dicaprylin + caprylate - ? 364128 3.1.1.3 (R,S)-2-O-butyryl-2-phenylacetate + H2O Enantioselectivity of the reaction can be obtained with various methods of enzyme immoblisation and reaction conditions Geobacillus thermocatenulatus (R)-2-O-butyryl-2-phenylacetate + (2S)-hydroxy(phenyl)ethanoic acid + butyrate - ? 399191 3.1.1.3 4-nitrophenyl butyrate + H2O - Geobacillus thermocatenulatus 4-nitrophenol + butyrate - ? 115376 3.1.1.3 4-nitrophenyl palmitate + H2O - Geobacillus thermocatenulatus 4-nitrophenol + palmitate - ? 363890 3.1.1.3 diethyl 3-phenylglutarate + H2O - Geobacillus thermocatenulatus (S)-ethyl phenylglutarate + (R)-ethyl phenylglutarate + ethanol - ? 400844 3.1.1.3 isophthalate + 2-butanol - Geobacillus thermocatenulatus isophthalic acid mono(1-methylpropyl)ester + isophthalic acid di(1-methylpropyl)ester + H2O the yield is 34% monoester and 46% diester in 1-butyl-3-methyl imidazolium tetrafluoroborate buffer ? 401193 3.1.1.3 isophthalate + cyclohexanol - Geobacillus thermocatenulatus isophthalic acid monocyclohexyl ester + H2O the yield is 7% monoester, no formation of diester in 1-butyl-3-methyl imidazolium tetrafluoroborate buffer ? 401194 3.1.1.3 isophthalate + cyclopentanol - Geobacillus thermocatenulatus isophthalic acid monocyclopentyl ester + H2O the yield is 5% monoester, no formation of diester in 1-butyl-3-methyl imidazolium tetrafluoroborate buffer ? 401195 3.1.1.3 isophthalate + ethanol - Geobacillus thermocatenulatus isophthalic acid monoethyl ester + isophthalic acid diethyl ester + H2O the yield is 74% monoester and no diester in 1-butyl-3-methyl imidazolium tetrafluoroborate buffer and 38% monoester and 24% diester in 1-butyl-3-methyl imidazolium hexafluorophosphate buffer respectively ? 401196 3.1.1.3 isophthalate + n-butanol - Geobacillus thermocatenulatus isophthalic acid monobutyl ester + H2O the yield is 14% for the monoester, no formation of diester in 1-butyl-3-methyl imidazolium tetrafluoroborate buffer ? 401197 3.1.1.3 phthalate + ethanol - Geobacillus thermocatenulatus phthalic acid monoethyl ester + phthalic acid diethyl ester + H2O the yield is 49% monoester and 30% diester in 1-butyl-3-methyl imidazolium tetrafluoroborate buffer and 67% monoester and 33% diester in 1-butyl-3-methyl imidazolium hexafluorophosphate buffer respectively ? 401752 3.1.1.3 terephthalate + ethanol - Geobacillus thermocatenulatus terephthalic acid monoethyl ester + terephthalic acid diethyl ester + H2O the yield is 44% monoester and no diester in 1-butyl-3-methyl imidazolium tetrafluoroborate buffer and 39% monoester and 51% diester in 1-butyl-3-methyl imidazolium hexafluorophosphate buffer respectively ? 401996 3.1.1.3 triacetin + H2O poor substrate Geobacillus thermocatenulatus diacetin + acetate - ? 364163 3.1.1.3 tributyrin + H2O best substrate Geobacillus thermocatenulatus dibutyrin + butyrate - ? 363949 3.1.1.3 tricaprin + H2O about 20% of the activity with tributyrin Geobacillus thermocatenulatus dicaprin + decaprate - ? 364146 3.1.1.3 tricaproin + H2O about 20% of the activity with tributyrin Geobacillus thermocatenulatus dicaproin + caproate - ? 364147 3.1.1.3 trilaurin + H2O about 10% of the activity with tributyrin Geobacillus thermocatenulatus dilaurin + laurate - ? 364081 3.1.1.3 trimyristin + H2O about 10% of the activity with tributyrin Geobacillus thermocatenulatus dimyristin + myristate - ? 364132 3.1.1.3 triolein + H2O - Geobacillus thermocatenulatus diolein + oleate - ? 363933 3.1.1.3 tripalmitin + H2O about 20% of the activity with tributyrin Geobacillus thermocatenulatus dipalmitin + palmitate - ? 364080 3.1.1.3 tristearin + H2O about 10% of the activity with tributyrin Geobacillus thermocatenulatus distearin + stearate - ? 364134 3.1.1.3 4-nitrophenyl palmitate + H2O - Geobacillus thermocatenulatus BTL2 4-nitrophenol + palmitate - ? 363890 3.1.1.3 tributyrin + H2O best substrate Geobacillus thermocatenulatus BTL2 dibutyrin + butyrate - ? 363949 3.1.1.3 trilaurin + H2O about 10% of the activity with tributyrin Geobacillus thermocatenulatus BTL2 dilaurin + laurate - ? 364081 3.1.1.3 triolein + H2O - Geobacillus thermocatenulatus BTL2 diolein + oleate - ? 363933 3.1.1.3 tripalmitin + H2O about 20% of the activity with tributyrin Geobacillus thermocatenulatus BTL2 dipalmitin + palmitate - ? 364080 3.1.1.3 1,2,3-trioleoyl-glycerol + H2O - Geobacillus thermoleovorans ? - ? 364148 3.1.1.3 1,2-dilinoleoyl-3-oleoyl-rac-glycerol + H2O - Geobacillus thermoleovorans ? - ? 364150 3.1.1.3 1,3-dioleoyl-2-palmitoyl-glycerol + H2O not BTID-A Geobacillus thermoleovorans ? - ? 364151 3.1.1.3 1,3-dipalmitoyl-2-oleoyl-glycerol + H2O - Geobacillus thermoleovorans ? - ? 364149 3.1.1.3 4-nitrophenyl butyrate + H2O - Geobacillus thermoleovorans 4-nitrophenol + butyrate - ? 115376 3.1.1.3 4-nitrophenyl caprate + H2O best 4-nitrophenyl ester substrate Geobacillus thermoleovorans 4-nitrophenol + caprate - ? 364083 3.1.1.3 4-nitrophenyl caproate + H2O - Geobacillus thermoleovorans 4-nitrophenol + caproate - ? 363887 3.1.1.3 4-nitrophenyl caprylate + H2O - Geobacillus thermoleovorans 4-nitrophenol + caprylate - ? 363889 3.1.1.3 4-nitrophenyl decanoate + H2O preferred substrate of the native mature enzyme Geobacillus thermoleovorans 4-nitrophenol + decanoate - ? 360778 3.1.1.3 4-nitrophenyl laurate + H2O - Geobacillus thermoleovorans 4-nitrophenol + laurate - ? 363891 3.1.1.3 4-nitrophenyl myristate + H2O - Geobacillus thermoleovorans 4-nitrophenol + myristate - ? 364082 3.1.1.3 4-nitrophenyl octanoate + H2O preferred substrate of the recombinant mature lipase Geobacillus thermoleovorans 4-nitrophenol + octanoate - ? 115380 3.1.1.3 4-nitrophenyl palmitate + H2O - Geobacillus thermoleovorans 4-nitrophenol + palmitate - ? 363890 3.1.1.3 4-nitrophenyl stearate + H2O - Geobacillus thermoleovorans 4-nitrophenol + stearate - ? 364135 3.1.1.3 additional information enzyme hydrolyzes triacylglycerides of plant oils such as olive oil, soybean oil, palm oil, and lard as carbon sources Geobacillus thermoleovorans ? - ? 89 3.1.1.3 additional information substrate specificity, methyl eicosanoate and methyl decosanoate are poor substrates, overview Geobacillus thermoleovorans ? - ? 89 3.1.1.3 triacetin + H2O - Geobacillus thermoleovorans diacetin + acetate - ? 364163 3.1.1.3 tributyrin + H2O - Geobacillus thermoleovorans dibutyrin + butyrate - ? 363949 3.1.1.3 tributyrin + H2O not BTID-A Geobacillus thermoleovorans dibutyrin + butyrate - ? 363949 3.1.1.3 tricaprin + H2O - Geobacillus thermoleovorans dicaprin + decaprate - ? 364146 3.1.1.3 tricaproin + H2O not BTID-A Geobacillus thermoleovorans dicaproin + caproate - ? 364147 3.1.1.3 tricaprylin + H2O best substrate, BTID-A and BTID-B Geobacillus thermoleovorans dicaprylin + caprylate - ? 364128 3.1.1.3 tricaprylin + H2O best triacylglyceride substrate Geobacillus thermoleovorans dicaprylin + caprylate - ? 364128 3.1.1.3 trilaurin + H2O - Geobacillus thermoleovorans dilaurin + laurate - ? 364081 3.1.1.3 trimyristin + H2O - Geobacillus thermoleovorans dimyristin + myristate - ? 364132 3.1.1.3 triolein + H2O olive oil Geobacillus thermoleovorans diolein + oleate - ? 363933 3.1.1.3 tripalmitin + H2O - Geobacillus thermoleovorans dipalmitin + palmitate - ? 364080 3.1.1.3 tripalmitin + H2O high activity Geobacillus thermoleovorans dipalmitin + palmitate - ? 364080 3.1.1.3 tristearin + H2O - Geobacillus thermoleovorans distearin + stearate - ? 364134 3.1.1.3 4-nitrophenyl decanoate + H2O preferred substrate of the native mature enzyme Geobacillus thermoleovorans CCR11 4-nitrophenol + decanoate - ? 360778 3.1.1.3 4-nitrophenyl laurate + H2O - Geobacillus thermoleovorans CCR11 4-nitrophenol + laurate - ? 363891 3.1.1.3 4-nitrophenyl octanoate + H2O preferred substrate of the recombinant mature lipase Geobacillus thermoleovorans CCR11 4-nitrophenol + octanoate - ? 115380 3.1.1.3 triolein + H2O olive oil Geobacillus thermoleovorans CCR11 diolein + oleate - ? 363933 3.1.1.3 additional information enzyme hydrolyzes triacylglycerides of plant oils such as olive oil, soybean oil, palm oil, and lard as carbon sources Geobacillus thermoleovorans ID-1 ? - ? 89 3.1.1.3 tributyrin + H2O not BTID-A Geobacillus thermoleovorans ID-1 dibutyrin + butyrate - ? 363949 3.1.1.3 tricaprylin + H2O best substrate, BTID-A and BTID-B Geobacillus thermoleovorans ID-1 dicaprylin + caprylate - ? 364128 3.1.1.3 trilaurin + H2O - Geobacillus thermoleovorans ID-1 dilaurin + laurate - ? 364081 3.1.1.3 tripalmitin + H2O - Geobacillus thermoleovorans ID-1 dipalmitin + palmitate - ? 364080 3.1.1.3 4-nitrophenyl butyrate + H2O - Geobacillus thermoleovorans YN 4-nitrophenol + butyrate - ? 115376 3.1.1.3 4-nitrophenyl caproate + H2O - Geobacillus thermoleovorans YN 4-nitrophenol + caproate - ? 363887 3.1.1.3 4-nitrophenyl caprylate + H2O - Geobacillus thermoleovorans YN 4-nitrophenol + caprylate - ? 363889 3.1.1.3 4-nitrophenyl palmitate + H2O - Geobacillus thermoleovorans YN 4-nitrophenol + palmitate - ? 363890 3.1.1.3 additional information substrate specificity, methyl eicosanoate and methyl decosanoate are poor substrates, overview Geobacillus thermoleovorans YN ? - ? 89 3.1.1.3 castor oil + H2O - Geotrichum candidum ? - ? 364069 3.1.1.3 corn oil + H2O - Geotrichum candidum ? - ? 364066 3.1.1.3 cotton seed oil + H2O - Geotrichum candidum ? - ? 391692 3.1.1.3 lard + H2O - Geotrichum candidum ? - ? 392466 3.1.1.3 additional information substrate specificity with natural oils and triacylglycerol substrates, overview, the enzyme catalyzes the hydrolysis of triacylglycerols into fatty acids and glycerol at the water-lipid interface and its reverse reaction in non-aqueous solvents Geotrichum candidum ? - ? 89 3.1.1.3 additional information the enzyme catalyzes the hydrolysis of triacylglycerols into fatty acids and glycerol at the water-lipid interface and its reverse reaction in non-aqueous solvents Geotrichum candidum ? - ? 89 3.1.1.3 additional information linolenic acid and behenic acid are not hydrolysed Geotrichum candidum ? - ? 89 3.1.1.3 olive oil + H2O - Geotrichum candidum ? - ? 364063 3.1.1.3 palm olein + H2O - Geotrichum candidum oleic acid + ? - ? 430583 3.1.1.3 rape seed oil + H2O - Geotrichum candidum ? - ? 393409 3.1.1.3 soybean oil + H2O - Geotrichum candidum ? - ? 364064 3.1.1.3 tea oil + H2O - Geotrichum candidum ? - ? 393643 3.1.1.3 triacylglycerol + H2O - Geotrichum candidum diacylglycerol + a carboxylate - ? 360693 3.1.1.3 tung oil + H2O - Geotrichum candidum ? - ? 393751 3.1.1.3 additional information linolenic acid and behenic acid are not hydrolysed Geotrichum candidum IMI 387428 ? - ? 89 3.1.1.3 palm olein + H2O - Geotrichum candidum IMI 387428 oleic acid + ? - ? 430583 3.1.1.3 triacylglycerol + H2O - Geotrichum candidum IMI 387428 diacylglycerol + a carboxylate - ? 360693 3.1.1.3 castor oil + H2O - Geotrichum candidum Y05 ? - ? 364069 3.1.1.3 corn oil + H2O - Geotrichum candidum Y05 ? - ? 364066 3.1.1.3 additional information substrate specificity with natural oils and triacylglycerol substrates, overview, the enzyme catalyzes the hydrolysis of triacylglycerols into fatty acids and glycerol at the water-lipid interface and its reverse reaction in non-aqueous solvents Geotrichum candidum Y05 ? - ? 89 3.1.1.3 olive oil + H2O - Geotrichum candidum Y05 ? - ? 364063 3.1.1.3 soybean oil + H2O - Geotrichum candidum Y05 ? - ? 364064 3.1.1.3 additional information substrate specificity, the extracellular enzyme prefers triacylglyceride substrates with cis double bonds in the fatty acid side chains, activity increases with number of double bonds, no activity with tristearin Geotrichum marinum ? - ? 89 3.1.1.3 trielaidin + H2O lower activity Geotrichum marinum dielaidin + elaidic acid - ? 382897 3.1.1.3 trilinolein + H2O good substrate Geotrichum marinum dilinolein + linoleate - ? 364139 3.1.1.3 trilinolenin + H2O preferred substrate Geotrichum marinum dilinolenin + linolenate - ? 364140 3.1.1.3 triolein + H2O good substrate Geotrichum marinum diolein + oleate - ? 363933 3.1.1.3 4-nitrophenyl acetate + H2O 12% of the activity with 4-nitrophenyl myristate Haloarcula sp. 4-nitrophenol + acetate - ? 12514 3.1.1.3 4-nitrophenyl butyrate + H2O 20% of the activity with 4-nitrophenyl myristate Haloarcula sp. 4-nitrophenol + butyrate - ? 115376 3.1.1.3 4-nitrophenyl decanoate + H2O 85% of the activity with 4-nitrophenyl myristate Haloarcula sp. 4-nitrophenol + decanoate - ? 360778 3.1.1.3 4-nitrophenyl hexanoate + H2O 30% of the activity with 4-nitrophenyl myristate Haloarcula sp. 4-nitrophenol + hexanoate - ? 115379 3.1.1.3 4-nitrophenyl laurate + H2O 95% of the activity with 4-nitrophenyl myristate Haloarcula sp. 4-nitrophenol + laurate - ? 363891 3.1.1.3 4-nitrophenyl myristate + H2O maximal activity among the tested 4-nitrophenyl esters Haloarcula sp. 4-nitrophenol + myristate - ? 364082 3.1.1.3 4-nitrophenyl octanoate + H2O 70% of the activity with 4-nitrophenyl myristate Haloarcula sp. 4-nitrophenol + octanoate - ? 115380 3.1.1.3 4-nitrophenyl palmitate + H2O 95% of the activity with 4-nitrophenyl myristate Haloarcula sp. 4-nitrophenol + palmitate - ? 363890 3.1.1.3 4-nitrophenyl stearate + H2O 90% of the activity with 4-nitrophenyl myristate Haloarcula sp. 4-nitrophenol + stearate - ? 364135 3.1.1.3 4-nitrophenyl acetate + H2O 12% of the activity with 4-nitrophenyl myristate Haloarcula sp. G41 4-nitrophenol + acetate - ? 12514 3.1.1.3 4-nitrophenyl butyrate + H2O 20% of the activity with 4-nitrophenyl myristate Haloarcula sp. G41 4-nitrophenol + butyrate - ? 115376 3.1.1.3 4-nitrophenyl decanoate + H2O 85% of the activity with 4-nitrophenyl myristate Haloarcula sp. G41 4-nitrophenol + decanoate - ? 360778 3.1.1.3 4-nitrophenyl hexanoate + H2O 30% of the activity with 4-nitrophenyl myristate Haloarcula sp. G41 4-nitrophenol + hexanoate - ? 115379 3.1.1.3 4-nitrophenyl octanoate + H2O 70% of the activity with 4-nitrophenyl myristate Haloarcula sp. G41 4-nitrophenol + octanoate - ? 115380 3.1.1.3 triacylglycerol + H2O the seed enzyme shows a preference for triglyceride with natural monounsaturated fatty acids Helianthus annuus diacylglycerol + a carboxylate - ? 360693 3.1.1.3 olive oil + H2O - Homarus americanus ? - ? 364063 3.1.1.3 tributyrin + H2O - Homarus americanus dibutyrin + butyrate - ? 363949 3.1.1.3 2,3-dibutyrylthio-1-propyl oleate + H2O chromogenic detection using 5,5'-dithio-bis-(2-nitrobenzoic acid) as chromogen or the 6-methylresorufin ester of 1-O,2-dilauryl-rac-glycero-3-glutaric acid, substrate synthesis, assay development, optimization, and method comparison, overview Homo sapiens 2,3-dibutyrylthiopropyl alcohol + oleate - ? 390586 3.1.1.3 4-nitrophenyl laurate + H2O - Homo sapiens 4-nitrophenol + laurate - ? 363891 3.1.1.3 4-nitrophenyl laurate + H2O recombinant enzyme in Sf9 cells Homo sapiens 4-nitrophenol + laurate - ? 363891 3.1.1.3 diolein + H2O - Homo sapiens monoolein + oleate - ? 400868 3.1.1.3 L-alpha-phosphatidylcholine + H2O - Homo sapiens ? - ? 401243 3.1.1.3 additional information enzyme does not possess protease activity and does not hydrolyze peptide bonds, no activity with N-benzoyl-L-tyrosine 4-nitroanilide Homo sapiens ? - ? 89 3.1.1.3 additional information regulation of gene expression Homo sapiens ? - ? 89 3.1.1.3 additional information measurement of in vivo enzyme activities by use of a liquid lipid containing test meal, determination of free fatty acids Homo sapiens ? - ? 89 3.1.1.3 additional information the enzyme mobilizes stored triacylglycerols some of which is used for very-low-density lipoprotein assembly in the liver Homo sapiens ? - ? 89 3.1.1.3 additional information the enzyme catalyzes the hydrolysis of triacylglycries into IDL and HDl, no co-linearitiy between insulin sensitivity and adiponectin as well as insulin sensitivity and enzyme activity, overview Homo sapiens ? - ? 89 3.1.1.3 additional information the enzyme is involved in basal lipolysis of storage triacylglycerides, and hormonally as well as developmentally regulated, physiological functions, role in apoB-containing lipoprotein assembly in the endoplasmic reticulum, overview Homo sapiens ? - ? 89 3.1.1.3 additional information the enzyme participates in VLDL assembly in endoplasmic reticulum without utilizing VLDL lipids as substrates Homo sapiens ? - ? 89 3.1.1.3 additional information substrate specificity of recombinant wild-type and mutant enzymes, overview Homo sapiens ? - ? 89 3.1.1.3 additional information the inactive enzyme mutant S152G binds to tricaprylin in a water emulsion interface competiting with the active enzyme hydrolyzing the substrate, overview Homo sapiens ? - ? 89 3.1.1.3 additional information the phospholipase A2 isozymes also show triacylglycerol lipase activity and acylglycerol transacylase activity Homo sapiens ? - ? 89 3.1.1.3 additional information Val407 and Ile408 in the beta5'-loop are required for interaction of the enzyme with the colipase in presence of bile salt micelles, overview Homo sapiens ? - ? 89 3.1.1.3 additional information the enzyme also shows phospholipase A1 activity, EC 3.1.1.32, galactolipase activity, EC 3.1.1.26, and acylglycerol lipase activity, EC 3.1.1.23, overview Homo sapiens ? - ? 89 3.1.1.3 additional information adipose TG lipase, ATGL, and hormone sensitive lipase, HSL, regulate lipolysis in a serial manner, with ATGL cleaving the first fatty acid and HSL the second fatty acid of triacylglycerol, lipolysis control, detailed overview Homo sapiens ? - ? 89 3.1.1.3 additional information ATGL selectively performs the first step in triacylglyceride hydrolysis resulting in the formation of diacylglyceride and free fatty acid. The specific activity against triacylglycerided is more than 10fold higher than against diacylglyceride, and the enzyme shows essentially no hydrolytic activity when other lipid substrates are used such as cholesteryl esters or retinyl esters. Molecular mechanisms that regulate ATGL activity, detailed overview Homo sapiens ? - ? 89 3.1.1.3 additional information structure, function, and regulation of ATGL and HSL, overview Homo sapiens ? - ? 89 3.1.1.3 additional information ATGL binds to the pigment epithelium derived factor Homo sapiens ? - ? 89 3.1.1.3 additional information ATGLalso possesses phospholipase and transacylase activity Homo sapiens ? - ? 89 3.1.1.3 olive oil + H2O - Homo sapiens ? - ? 364063 3.1.1.3 triacylglycerol + H2O - Homo sapiens diacylglycerol + a carboxylate - ? 360693 3.1.1.3 triacylglycerol + H2O ATGL plays a pivotal role for ATGL in intramuscular fatty acid handling, lipid storage and breakdown, overview, disturbances in skeletal muscle lipid turnover and lipolysis lead to accumulation of triacylglycerol and lipid intermediates in skeletal muscle, which plays an important role in the etiology of insulin resistance and type 2 diabetes mellitus Homo sapiens diacylglycerol + a carboxylate - ? 360693 3.1.1.3 triacylglycerol + H2O endothelial lipase plays a central role in plasma lipoprotein metabolism Homo sapiens diacylglycerol + a carboxylate - ? 360693 3.1.1.3 triacylglycerol + H2O the endothelial lipase is more active as a phospholipase than as a triacylglyceride lipase Homo sapiens diacylglycerol + a carboxylate - ? 360693 3.1.1.3 tributyrin + H2O - Homo sapiens butyric acid + ? - ? 64394 3.1.1.3 tributyrin + H2O - Homo sapiens ? - ? 64455 3.1.1.3 tributyrin + H2O - Homo sapiens dibutyrin + butyrate - ? 363949 3.1.1.3 tributyrin + H2O nonphysiologic substrate Homo sapiens dibutyrin + butyrate - ? 363949 3.1.1.3 tricaprylin + H2O - Homo sapiens dicaprylin + caprylate - ? 364128 3.1.1.3 trioctanoin + H2O - Homo sapiens octanoic acid + ? - ? 64398 3.1.1.3 trioctanoin + H2O - Homo sapiens dioctanoin + octanoate - ? 393712 3.1.1.3 triolein + H2O - Homo sapiens diolein + oleate - ? 363933 3.1.1.3 triolein + H2O substrate is an olive oil/gum arabic emulsion containing radiolabeled triolein in presence of bile acids and lecithin at rate-limiting concentrations, rapid exchange of the enzyme between two different triacylglycerol droplets measured by a non-exchanging enzyme inhibitor orlistat in one of the droplets Homo sapiens diolein + oleate - ? 363933 3.1.1.3 trioleoylglycerol + H2O - Homo sapiens oleic acid + ? - ? 64389 3.1.1.3 triolein + H2O olive oil Hyalomma dromedarii diolein + oleate - ? 363933 3.1.1.3 tributyrin + H2O - Hyphopichia burtonii dibutyrin + butyrate - ? 363949 3.1.1.3 4-nitrophenyl butyrate + H2O - Jacaranda mimosifolia 4-nitrophenol + butyrate - ? 115376 3.1.1.3 Tween 20 + H2O - Jacaranda mimosifolia ? - ? 364141 3.1.1.3 Tween 80 + H2O - Jacaranda mimosifolia ? - ? 364144 3.1.1.3 4-nitrophenyl laurate + H2O - Liza parsia 4-nitrophenol + laurate - ? 363891 3.1.1.3 olive oil + H2O - Liza parsia ? - ? 364063 3.1.1.3 4-nitrophenyl caprate + H2O - Lymantria dispar 4-nitrophenol + caprate - ? 364083 3.1.1.3 4-nitrophenyl caprylate + H2O best substrate Lymantria dispar 4-nitrophenol + caprylate - ? 363889 3.1.1.3 4-nitrophenyl laurate + H2O - Lymantria dispar 4-nitrophenol + laurate - ? 363891 3.1.1.3 additional information enzymatic activity chain fatty acid esters longer than C12 is below 10% of maximum activity Lymantria dispar ? - ? 89 3.1.1.3 4-methylumbelliferyl butyrate + H2O - Macruronus novaezelandiae 4-methylumbelliferone + butyrate - ? 73359 3.1.1.3 4-methylumbelliferyl oleate + H2O - Macruronus novaezelandiae 4-methylumbelliferone + oleate - ? 416332 3.1.1.3 4-nitrophenyl acetate + H2O - Macruronus novaezelandiae 4-nitrophenol + acetate - ? 12514 3.1.1.3 4-nitrophenyl caprate + H2O best substrate Macruronus novaezelandiae 4-nitrophenol + caprate - ? 364083 3.1.1.3 4-nitrophenyl caprate + H2O highest lipase activity is obtained with 0.25 mM of the substrate Macruronus novaezelandiae 4-nitrophenol + caprate - ? 364083 3.1.1.3 4-nitrophenyl palmitate + H2O highest lipase activity is obtained with 0.25 mM of the substrate Macruronus novaezelandiae 4-nitrophenol + palmitate - ? 363890 3.1.1.3 4-nitrophenyl butanoate + H2O 20% of the activity with -nitrophenyl myristate Malbranchea cinnamomea 4-nitrophenol + butanoate - ? 363828 3.1.1.3 4-nitrophenyl decanoate + H2O 81% of the activity with -nitrophenyl myristate Malbranchea cinnamomea 4-nitrophenol + decanoate - ? 360778 3.1.1.3 4-nitrophenyl myristate + H2O - Malbranchea cinnamomea 4-nitrophenol + myristate - ? 364082 3.1.1.3 4-nitrophenyl palmitate + H2O 54% of the activity with -nitrophenyl myristate Malbranchea cinnamomea 4-nitrophenol + palmitate - ? 363890 3.1.1.3 glyceryl trioleate + H2O - Malbranchea cinnamomea glycerol 1,2-dioleate + oleate - ? 441424 3.1.1.3 additional information enzyme is active toward 4-nitrophenyl esters of various carbon chain lengths with peak activity on long-chain fatty acid (C14). It displays high sn-1,3-regioselectivity on hydrolyzing triolein Malbranchea cinnamomea ? - ? 89 3.1.1.3 oleate + methanol - Malbranchea cinnamomea oleic acid methyl ester - ? 441801 3.1.1.3 triacylglycerol + H2O - Manduca sexta diacylglycerol + a carboxylate - ? 360693 3.1.1.3 triacylglycerol + H2O the enzyme is involved in adipokinetic hormone-induced mobilization of fat body triglyceride stores in Manduca sexta, protein kinase A phosphorylates and activates the TG-lipase substrate, the lipid droplets, protein kinase A also phosphorylates the lipase, but does not directly activate it, overview Manduca sexta diacylglycerol + a carboxylate - ? 360693 3.1.1.3 triolein + H2O - Manduca sexta diolein + oleate - ? 363933 3.1.1.3 additional information the enzyme exhibits efficient hydrolysis of oilive oil in the absence of colipase and bile salts Meleagris gallopavo ? - ? 89 3.1.1.3 tributyrin + H2O best substrate, efficient hydrolysis in the absence of colipase and bile salts Meleagris gallopavo dibutyrin + butyrate - ? 363949 3.1.1.3 alpha-naphthyl butyrate + H2O preferred substrate Micrococcus sp. alpha-naphthol + butyrate - ? 363970 3.1.1.3 alpha-naphthyl caprylate + H2O 58% of the activity with the alpha-isomer Micrococcus sp. alpha-naphthol + caprylate - ? 363972 3.1.1.3 alpha-naphthyl laurate + H2O - Micrococcus sp. alpha-naphthol + laurate - ? 364195 3.1.1.3 alpha-naphthyl stearate + H2O equally active with both isomers Micrococcus sp. alpha-naphthol + stearate - ? 364197 3.1.1.3 beta-naphthyl butyrate + H2O 58% of the activity with the alpha-isomer Micrococcus sp. beta-naphthol + butyrate - ? 364193 3.1.1.3 beta-naphthyl caprylate + H2O - Micrococcus sp. beta-naphthol + caprylate - ? 364194 3.1.1.3 beta-naphthyl laurate + H2O 62.5% of the activity with the alpha-isomer Micrococcus sp. beta-naphthol + laurate - ? 364196 3.1.1.3 beta-naphthyl stearate + H2O equally active with both isomers Micrococcus sp. beta-naphthol + stearate - ? 457022 3.1.1.3 additional information the enzyme hydrolyzes alpha-naphthyl esters of fatty acid with chain lengths from C4 to C18 Micrococcus sp. ? - ? 89 3.1.1.3 tributyrin + H2O best substrate Micrococcus sp. dibutyrin + butyrate - ? 363949 3.1.1.3 tricaprylin + H2O - Micrococcus sp. dicaprylin + caprylate - ? 364128 3.1.1.3 trilaurin + H2O - Micrococcus sp. dilaurin + laurate - ? 364081 3.1.1.3 alpha-naphthyl butyrate + H2O preferred substrate Micrococcus sp. INIA 528 alpha-naphthol + butyrate - ? 363970 3.1.1.3 alpha-naphthyl caprylate + H2O 58% of the activity with the alpha-isomer Micrococcus sp. INIA 528 alpha-naphthol + caprylate - ? 363972 3.1.1.3 additional information the enzyme hydrolyzes alpha-naphthyl esters of fatty acid with chain lengths from C4 to C18 Micrococcus sp. INIA 528 ? - ? 89 3.1.1.3 tributyrin + H2O best substrate Micrococcus sp. INIA 528 dibutyrin + butyrate - ? 363949 3.1.1.3 trilaurin + H2O - Micrococcus sp. INIA 528 dilaurin + laurate - ? 364081 3.1.1.3 (R)-2-ethylhexanoic acid ethyl ester + H2O - Moesziomyces antarcticus (R)-2-ethylhexanoate + ethanol - ? 415929 3.1.1.3 (R)-3,5,5-trimethylhexanoic acid ethyl ester + H2O - Moesziomyces antarcticus (R)-3,5,5-trimethylhexanoate + ethanol - ? 415930 3.1.1.3 (S)-2-ethyl hexanoic acid ethyl ester + H2O - Moesziomyces antarcticus ? - ? 415943 3.1.1.3 (S)-3,5,5-trimethylhexanoic acid ethyl ester + H2O - Moesziomyces antarcticus (S)-3,5,5-trimethylhexanoate + ethanol - ? 415946 3.1.1.3 2-ethylhexanoic acid ethyl ester + H2O lowest activity Moesziomyces antarcticus ? - ? 416131 3.1.1.3 2-[2-(2,4-difluoro-phenyl)-allyl]-propane-1,3-diol + vinyl acetate - Moesziomyces antarcticus (S)-acetic acid 4-(2,4-difluoro-phenyl)-2-hydroxymethyl-pent-4-enyl ester + acetic acid 2-acetoxymethyl-4-(2,4-difluoro-phenyl)-pent-4-enyl-ester - ? 375709 3.1.1.3 4-nitrophenyl 2-(4-methylphenyl)propanoate + H2O 55.6% conversion, (S)-selectivity Moesziomyces antarcticus 4-nitrophenol + 2-(4-methylphenyl)propanoate - ? 416343 3.1.1.3 4-nitrophenyl 2-benzylpropanoate + H2O 80.3% conversion, (S)-selectivity Moesziomyces antarcticus 4-nitrophenol + 2-benzylpropanoate - ? 416346 3.1.1.3 4-nitrophenyl 2-ethylhexanoate + H2O 75.3% conversion, (S)-selectivity Moesziomyces antarcticus 4-nitrophenol + 2-ethylhexanoate - ? 416347 3.1.1.3 4-nitrophenyl 2-methylheptanoate + H2O 80.7% conversion, (S)-selectivity Moesziomyces antarcticus 4-nitrophenol + 2-methylheptanoate - ? 416350 3.1.1.3 4-nitrophenyl 2-phenylbutanoate + H2O 17% conversion, (R)-selectivity Moesziomyces antarcticus 4-nitrophenol + 2-phenylbutanoate - ? 416353 3.1.1.3 4-nitrophenyl 2-phenylpentanoate + H2O 88.1% conversion, (R)-selectivity Moesziomyces antarcticus 4-nitrophenol + 2-phenylpentanoate - ? 416354 3.1.1.3 4-nitrophenyl 2-phenylpropanoate + H2O 38% conversion, (S)-selectivity Moesziomyces antarcticus 4-nitrophenol + 2-phenylpropanoate - ? 416355 3.1.1.3 4-nitrophenyl butyrate + H2O - Moesziomyces antarcticus 4-nitrophenol + butyrate - ? 115376 3.1.1.3 4-nitrophenyl palmitate + H2O - Moesziomyces antarcticus 4-nitrophenol + palmitate - ? 363890 3.1.1.3 diethyl malate + H2O - Moesziomyces antarcticus ? - ? 416903 3.1.1.3 diethyl tartrate + H2O - Moesziomyces antarcticus ? - ? 416907 3.1.1.3 dimethyl succinate + H2O high activity Moesziomyces antarcticus ? - ? 416925 3.1.1.3 ethyl L-lactate + H2O - Moesziomyces antarcticus ethanol + L-lactate - ? 416989 3.1.1.3 glycerol + 1,8-octanediol + adipic acid polycondensation Moesziomyces antarcticus ? - ? 401069 3.1.1.3 isononanoic acid ethyl ester + H2O - Moesziomyces antarcticus isononanoic acid + ethanol - ? 417117 3.1.1.3 L-lysine ethyl ester + H2O - Moesziomyces antarcticus L-lysine + ethanol - ? 417163 3.1.1.3 methyl caprylate + H2O best substrate Moesziomyces antarcticus methanol + caprylate - ? 364185 3.1.1.3 methyl laurate + H2O - Moesziomyces antarcticus methanol + laurate - ? 364077 3.1.1.3 additional information lipases A and B display no interfacial activation due to absence of the lid structure which regulates the access to the active site. The enzyme performs estrification of lactic acid and alcohols in hexane Moesziomyces antarcticus ? - ? 89 3.1.1.3 additional information CalA shows preference for short-chain triglycerides, low activity with hydrophilic esters Moesziomyces antarcticus ? - ? 89 3.1.1.3 additional information the enzyme performs a reversible monoglyceride isomerization, the rate of iso-propanolysis reactions is higher than propanolysis, mechanism of 1,3-specific alcoholysis, overview Moesziomyces antarcticus ? - ? 89 3.1.1.3 additional information The conversion of the lauric acid is at 80% after 4 h whereas the residual amount of polyglycerol is at 50% at this time. The conversion of polyglycerol remains lower than the conversion of lauric acid, because of the multiple hydroxy functions within the mixture of different glycerol oligomers. The equilibrium of the reaction can be shifted to total conversion by removal of water applying vacuum to the stirred tank reactor. Moesziomyces antarcticus ? - ? 89 3.1.1.3 additional information The synthesis of myristyl myristate by Sepabeads EC-EP/CALB is an example for a biocatalytic surfactant production. Moesziomyces antarcticus ? - ? 89 3.1.1.3 additional information lipase B can only convert straight chain fatty acids of different length with a preference toward C5 and C12 Moesziomyces antarcticus ? - ? 89 3.1.1.3 additional information the enzyme catalyzes the alcoholysis of soybean oil with ethanol in propane medium Moesziomyces antarcticus ? - ? 89 3.1.1.3 palmitic acid + isopropanol - Moesziomyces antarcticus isopropyl palmitate + H2O - ? 377455 3.1.1.3 R-ketoprofen + H2O lipase B is stereoselective for the R-isomer in an antichiral solvent such as isopentyl methyl ketone or S(+)-carvone Moesziomyces antarcticus ? - ? 364090 3.1.1.3 triacetin + H2O - Moesziomyces antarcticus diacetin + acetate - ir 364163 3.1.1.3 tributyrin + H2O - Moesziomyces antarcticus dibutyrin + butyrate - ? 363949 3.1.1.3 tricaprylin + H2O preferred substrate Moesziomyces antarcticus dicaprylin + caprylate - ir 364128 3.1.1.3 triethyl citrate + H2O - Moesziomyces antarcticus ? - ? 417718 3.1.1.3 triolein + cinnamic acid transesterification Moesziomyces antarcticus monocinnamoyl oleoyl glycerol + dicinnamoyl oleoyl glyerol + monocinnamoyl dioleoyl glycerol - ? 402046 3.1.1.3 triolein + ferulic acid transesterification Moesziomyces antarcticus monoferuloyl oleoyl glycerol + monoferuloyl dioleoyl glycerol - ? 402047 3.1.1.3 high linoleic sunflower oil + H2O - Mucor javanicus ? - ? 401143 3.1.1.3 high oleic sunflower oil + H2O - Mucor javanicus ? - ? 401146 3.1.1.3 additional information lipolysis is regulated by the opposing functions of the enzyme ATGL and adiponutrin, overview Mus musculus ? - ? 89 3.1.1.3 additional information the enzyme is involved in basal lipolysis of storage triacylglycerides, and hormonally as well as developmentally regulated, the hormone-sensitive lipase is negatively regulated by perilipin in adipose tissue, physiological functions, role in apoB-containing lipoprotein assembly in the endoplasmic reticulum, overview Mus musculus ? - ? 89 3.1.1.3 additional information ATGL and diacylglycerol acyltransferase-1, DGAT-1, may be cooperatively involved in rosiglitazone-stimulated triglyceride hydrolysis and fatty acid re-esterification in 3T3-L1 adipocytes, overview Mus musculus ? - ? 89 3.1.1.3 additional information ATGL and hormone sensitive lipase, HSL, regulate lipolysis in a serial manner, with ATGL cleaving the first fatty acid and HSL the second fatty acid of triacylglycerol, lipolysis control, detailed overview Mus musculus ? - ? 89 3.1.1.3 additional information ATGL selectively performs the first step in triacylglyceride hydrolysis resulting in the formation of diacylglyceride and free fatty acid. The specific activity against triacylglycerides is more than 10fold higher than against diacylglyceride, and the enzyme shows essentially no hydrolytic activity when other lipid substrates are used such as cholesteryl esters or retinyl esters. Molecular mechanisms that regulate ATGL activity, detailed overview Mus musculus ? - ? 89 3.1.1.3 additional information HSL translocates from the cytoplasm to the lipid droplet surface and interacts with Peri A in stimulated lipolysis Mus musculus ? - ? 89 3.1.1.3 additional information structure, function, and regulation of ATGL and HSL, overview Mus musculus ? - ? 89 3.1.1.3 additional information ATGL binds to the pigment epithelium derived factor Mus musculus ? - ? 89 3.1.1.3 additional information ATGLalso possesses phospholipase and transacylase activity Mus musculus ? - ? 89 3.1.1.3 additional information the enzyme hydrolyzes long-chain fatty acid esters in vivo with a modest substrate preference for C16:1 Mus musculus ? - ? 89 3.1.1.3 additional information the enzyme lacks the ability to hydrolyze diacylglycerols, monoacylglycerols, cholesteryl esters, or retinyl esters Mus musculus ? - ? 89 3.1.1.3 additional information the enzyme lacks the ability to hydrolyze monoacylglycerol, CEs, or retinyl esters Mus musculus ? - ? 89 3.1.1.3 olive oil + H2O - Mus musculus ? - ? 364063 3.1.1.3 retinyl palmitate + H2O - Mus musculus retinol + palmitate - ? 115251 3.1.1.3 triacylglycerol + H2O - Mus musculus diacylglycerol + a carboxylate - ? 360693 3.1.1.3 triacylglycerol + H2O - Mus musculus diacylglycerol + a carboxylate the enzyme specifically generates sn-1,3 and, in the presence of its co-activator CGI-58, sn-1,3 and sn-2,3 diacylglycerol ? 360693 3.1.1.3 trilinolein + H2O lowest activity Mus musculus ? - ? 417720 3.1.1.3 trilinolenin + H2O - Mus musculus ? - ? 417721 3.1.1.3 triolein + H2O - Mus musculus ? - ? 64454 3.1.1.3 triolein + H2O - Mus musculus diolein + oleic acid - ? 364170 3.1.1.3 tripalmitolein + H2O highest activity Mus musculus ? - ? 417725 3.1.1.3 additional information the enzyme hydrolyzes long-chain fatty acid esters in vivo with a modest substrate preference for C16:1 Mus musculus C57BL/6 ? - ? 89 3.1.1.3 triacylglycerol + H2O - Mus musculus C57BL/6 diacylglycerol + a carboxylate the enzyme specifically generates sn-1,3 and, in the presence of its co-activator CGI-58, sn-1,3 and sn-2,3 diacylglycerol ? 360693 3.1.1.3 trilinolein + H2O lowest activity Mus musculus C57BL/6 ? - ? 417720 3.1.1.3 trilinolenin + H2O - Mus musculus C57BL/6 ? - ? 417721 3.1.1.3 triolein + H2O - Mus musculus C57BL/6 ? - ? 64454 3.1.1.3 diolein + H2O - Mycobacterium tuberculosis 1-oleoyl-sn-glycerol + oleate - ? 381810 3.1.1.3 triolein + H2O - Mycobacterium tuberculosis diolein + oleate - ? 363933 3.1.1.3 diolein + H2O - Mycobacterium tuberculosis H37Rv 1-oleoyl-sn-glycerol + oleate - ? 381810 3.1.1.3 triolein + H2O - Mycobacterium tuberculosis H37Rv diolein + oleate - ? 363933 3.1.1.3 additional information the enzyme prefers triglycerides with C16-fatty acyl chains and C18-fatty acyl chains, it cleaves only the primary groups of triglycerides, marked preference for substrates containing endogenously occuring fatty acids Neurospora crassa ? - ? 89 3.1.1.3 trilinolein + H2O - Neurospora crassa linolic acid + ? - ? 64407 3.1.1.3 trioleoylglycerol + H2O - Neurospora crassa oleic acid + ? - ? 64389 3.1.1.3 tripalmitin + H2O - Neurospora crassa palmitic acid + ? - ? 64402 3.1.1.3 tristearin + H2O - Neurospora crassa stearic acid + ? - ? 64403 3.1.1.3 triolein + H2O - Olea europaea diolein + oleate - ? 363933 3.1.1.3 4-methylumbelliferyl butyrate + H2O - Oncorhynchus tshawytscha 4-methylumbelliferone + butyrate - ? 73359 3.1.1.3 4-methylumbelliferyl oleate + H2O - Oncorhynchus tshawytscha 4-methylumbelliferone + oleate - ? 416332 3.1.1.3 4-nitrophenyl acetate + H2O - Oncorhynchus tshawytscha 4-nitrophenol + acetate - ? 12514 3.1.1.3 4-nitrophenyl caprate + H2O best substrate Oncorhynchus tshawytscha 4-nitrophenol + caprate - ? 364083 3.1.1.3 4-nitrophenyl caprate + H2O highest lipase activity is obtained with 0.25 mM of the substrate Oncorhynchus tshawytscha 4-nitrophenol + caprate - ? 364083 3.1.1.3 4-nitrophenyl palmitate + H2O highest lipase activity is obtained with 0.25 mM of the substrate Oncorhynchus tshawytscha 4-nitrophenol + palmitate - ? 363890 3.1.1.3 4-methylumbelliferyl ester + H2O with fatty acids of different length in decreasing order, medium chain, long chains, short chains Oryctolagus cuniculus 4-methylumbelliferone + fatty acid - ? 64436 3.1.1.3 4-methylumbelliferyl oleate + H2O - Oryctolagus cuniculus 4-methylumbelliferone + oleic acid - ? 64435 3.1.1.3 cholesterol oleate + H2O - Oryctolagus cuniculus cholesterol + oleic acid - ? 64434 3.1.1.3 diolein + H2O - Oryctolagus cuniculus oleic acid + ? - ? 64390 3.1.1.3 monoolein + H2O - Oryctolagus cuniculus oleic acid + glycerol - ? 64391 3.1.1.3 additional information the enzyme is involved in the hydrolysis of both acylglycerols and cholesterol esters in lysosomes Oryctolagus cuniculus ? - ? 89 3.1.1.3 tributyrin + H2O - Oryctolagus cuniculus butyric acid + ? - ? 64394 3.1.1.3 trioleoylglycerol + H2O - Oryctolagus cuniculus oleic acid + ? - ? 64389 3.1.1.3 1-naphthyl acetate + H2O 36.9% activity compared to rice bran oil Oryza sativa 1-naphthol + acetate - ? 12515 3.1.1.3 1-naphthyl butyrate + H2O 18.8% activity compared to rice bran oil Oryza sativa 1-naphthol + butyrate - ? 115402 3.1.1.3 4-nitrophenyl acetate + H2O 0.2% activity compared to rice bran oil Oryza sativa 4-nitrophenol + acetate - ? 12514 3.1.1.3 coconut oil + H2O 47.9% activity compared to rice bran oil Oryza sativa ? - ? 364067 3.1.1.3 diacylglycerol + H2O - Oryza sativa fatty acid + acylglycerol - ? 364161 3.1.1.3 additional information enzyme also provides phospholipase A2 activity, it preferentially hydrolyzes the sn-2-position of the substrate Oryza sativa ? - ? 89 3.1.1.3 olive oil + H2O 95.3% activity compared to rice bran oil Oryza sativa ? - ? 364063 3.1.1.3 palm oil + H2O 77.7% activity compared to rice bran oil Oryza sativa ? - ? 430582 3.1.1.3 phosphatidylethanolamine + H2O - Oryza sativa ? - ? 64438 3.1.1.3 phosphocholine + H2O - Oryza sativa ? - ? 452522 3.1.1.3 rice bran oil 100% activity Oryza sativa ? - ? 430673 3.1.1.3 sunflower oil + H2O 91.7% activity compared to rice bran oil Oryza sativa ? - ? 364065 3.1.1.3 triacetin + H2O 120% activity compared to rice bran oil Oryza sativa ? - ? 73241 3.1.1.3 tributyrin + H2O 92.6% activity compared to rice bran oil Oryza sativa ? - ? 64455 3.1.1.3 trimyristin + H2O 90.1% activity compared to rice bran oil Oryza sativa ? - ? 430740 3.1.1.3 triolein + H2O enzyme shows no positional specificity towards the triacylglycerol Oryza sativa diolein + oleate - ? 363933 3.1.1.3 tripalmitin + H2O 82.1% activity compared to rice bran oil Oryza sativa ? - ? 413001 3.1.1.3 tristearin + H2O 72.9% activity compared to rice bran oil Oryza sativa ? - ? 413004 3.1.1.3 1-naphthyl acetate + H2O - Penaeus vannamei 1-naphthol + acetate - ? 12515 3.1.1.3 2-naphthyl acetate + H2O - Penaeus vannamei 2-naphthol + acetate - ? 36028 3.1.1.3 2-naphthyl caprylate + H2O - Penaeus vannamei 2-naphthol + caprylate - ? 416149 3.1.1.3 4-methylumbelliferyl butyrate + H2O - Penaeus vannamei 4-methylumbelliferone + butyrate - ? 73359 3.1.1.3 triolein + H2O - Penaeus vannamei ? - ? 64454 3.1.1.3 tripalmitin + H2O - Penaeus vannamei ? - ? 413001 3.1.1.3 tristearin + H2O - Penaeus vannamei ? - ? 413004 3.1.1.3 4-nitrophenyl butyrate + H2O - Penicillium aurantiogriseum 4-nitrophenol + butyrate - ? 115376 3.1.1.3 4-nitrophenyl caprate + H2O best substrate Penicillium aurantiogriseum 4-nitrophenol + caprate - ? 364083 3.1.1.3 4-nitrophenyl caproate + H2O - Penicillium aurantiogriseum 4-nitrophenol + caproate - ? 363887 3.1.1.3 4-nitrophenyl palmitate + H2O - Penicillium aurantiogriseum 4-nitrophenol + palmitate - ? 363890 3.1.1.3 additional information no activity with 4-nitrophenyl acetate Penicillium aurantiogriseum ? - ? 89 3.1.1.3 tributyrin + H2O - Penicillium aurantiogriseum dibutyrin + butyrate - ? 363949 3.1.1.3 triolein + H2O - Penicillium aurantiogriseum diolein + oleate - ? 363933 3.1.1.3 high linoleic sunflower oil + H2O - Penicillium camemberti ? - ? 401143 3.1.1.3 high oleic sunflower oil + H2O - Penicillium camemberti ? - ? 401146 3.1.1.3 4-nitrophenyl butyrate + H2O low activity Penicillium candidum 4-nitrophenol + butyrate - ? 115376 3.1.1.3 4-nitrophenyl decanoate + H2O - Penicillium candidum 4-nitrophenol + decanoate - ? 360778 3.1.1.3 4-nitrophenyl hexanoate + H2O low activity Penicillium candidum 4-nitrophenol + hexanoate - ? 115379 3.1.1.3 4-nitrophenyl laurate + H2O - Penicillium candidum 4-nitrophenol + laurate - ? 363891 3.1.1.3 4-nitrophenyl octanoate + H2O low activity Penicillium candidum 4-nitrophenol + octanoate - ? 115380 3.1.1.3 4-nitrophenyl palmitate + H2O best substrate Penicillium candidum 4-nitrophenol + palmitate - ? 363890 3.1.1.3 4-nitrophenyl stearate + H2O - Penicillium candidum 4-nitrophenol + stearate - ? 364135 3.1.1.3 additional information substrate specificity Penicillium candidum ? - ? 89 3.1.1.3 tributyrin + H2O best substrate Penicillium candidum dibutyrin + butyrate - ? 363949 3.1.1.3 tridecanin + H2O - Penicillium candidum didecanin + decanoate - ? 364130 3.1.1.3 tridodecanin + H2O - Penicillium candidum didodecanin + dodecanoate - ? 364131 3.1.1.3 trihexanin + H2O - Penicillium candidum dihexanin + hexanoate - ? 364129 3.1.1.3 trimyristin + H2O - Penicillium candidum dimyristin + myristate - ? 364132 3.1.1.3 triolein + H2O - Penicillium candidum diolein + oleate - ? 363933 3.1.1.3 tripalmitin + H2O - Penicillium candidum dipalmitin + palmitate - ? 364080 3.1.1.3 tristearin + H2O - Penicillium candidum distearin + stearate - ? 364134 3.1.1.3 methyl linoleate + H2O - Penicillium cyclopium methanol + linolic acid - ? 64427 3.1.1.3 methyl linolenate + H2O - Penicillium cyclopium methanol + linoleic acid - ? 64428 3.1.1.3 methyl oleate + H2O - Penicillium cyclopium methanol + oleic acid - ? 64426 3.1.1.3 methyl ricinoleate + H2O - Penicillium cyclopium methanol + ricinoleic acid - ? 64429 3.1.1.3 additional information highly active against all triacylglycerols and very low activity against partial acylglycerols or monoesters Penicillium cyclopium ? - ? 89 3.1.1.3 polyethylene sorbitan monooleate + H2O - Penicillium cyclopium ? - ? 64431 3.1.1.3 Span 85 + H2O - Penicillium cyclopium ? - ? 64432 3.1.1.3 tributyrin + H2O - Penicillium cyclopium butyric acid + ? - ? 64394 3.1.1.3 trihexanoin + H2O - Penicillium cyclopium hexanoic acid + ? - ? 64397 3.1.1.3 trilaurin + H2O - Penicillium cyclopium lauric acid + ? - ? 64396 3.1.1.3 trioctanoin + H2O - Penicillium cyclopium octanoic acid + ? - ? 64398 3.1.1.3 trioleoylglycerol + H2O - Penicillium cyclopium oleic acid + ? - ? 64389 3.1.1.3 tripalmitin + H2O - Penicillium cyclopium palmitic acid + ? - ? 64402 3.1.1.3 tributyrin + H2O - Penicillium cyclopium M1 butyric acid + ? - ? 64394 3.1.1.3 trihexanoin + H2O - Penicillium cyclopium M1 hexanoic acid + ? - ? 64397 3.1.1.3 trilaurin + H2O - Penicillium cyclopium M1 lauric acid + ? - ? 64396 3.1.1.3 trioleoylglycerol + H2O - Penicillium cyclopium M1 oleic acid + ? - ? 64389 3.1.1.3 tripalmitin + H2O - Penicillium cyclopium M1 palmitic acid + ? - ? 64402 3.1.1.3 (R,S)-ibuprofen + 1-propanol purified enzyme in a mixture of 1-propanol-isooctane in a ratio of 3:1, the conversion of racemic ibuprofen reaches 46% with very high enantioselectivity for production of the (S)-enantiomer Penicillium expansum (S)-ibuprofen n-propyl ester + (R)-ibuprofen - ? 390463 3.1.1.3 4-nitrophenyl palmitate + H2O - Penicillium expansum 4-nitrophenol + palmitate - ? 363890 3.1.1.3 (R,S)-ibuprofen + 1-propanol purified enzyme in a mixture of 1-propanol-isooctane in a ratio of 3:1, the conversion of racemic ibuprofen reaches 46% with very high enantioselectivity for production of the (S)-enantiomer Penicillium expansum PED-03 (S)-ibuprofen n-propyl ester + (R)-ibuprofen - ? 390463 3.1.1.3 4-nitrophenyl palmitate + H2O - Penicillium expansum PED-03 4-nitrophenol + palmitate - ? 363890 3.1.1.3 high linoleic sunflower oil + H2O - Penicillium roqueforti ? - ? 401143 3.1.1.3 high oleic sunflower oil + H2O - Penicillium roqueforti ? - ? 401146 3.1.1.3 additional information highly specific for short-chain fatty acids esters Penicillium roqueforti ? - ? 89 3.1.1.3 tributyrin + H2O - Penicillium roqueforti dibutyrin + butyrate - ? 363949 3.1.1.3 additional information highly specific for short-chain fatty acids esters Penicillium roqueforti IAM7268 ? - ? 89 3.1.1.3 tributyrin + H2O - Penicillium roqueforti IAM7268 dibutyrin + butyrate - ? 363949 3.1.1.3 cane molasse + H2O - Penicillium verrucosum ? - ? 400604 3.1.1.3 castor oil + H2O - Penicillium verrucosum ? - ? 364069 3.1.1.3 corn oil + H2O - Penicillium verrucosum ? - ? 364066 3.1.1.3 corn steep liquor + H2O - Penicillium verrucosum ? - ? 400698 3.1.1.3 olive oil + H2O - Penicillium verrucosum ? - ? 364063 3.1.1.3 soybean bran + H2O - Penicillium verrucosum ? - ? 401900 3.1.1.3 soybean oil + H2O - Penicillium verrucosum ? - ? 364064 3.1.1.3 yeast hydrolysate + H2O - Penicillium verrucosum ? - ? 402262 3.1.1.3 tributyrin + H2O - Penicillium wortmanii dibutyrin + butyrate - ? 363949 3.1.1.3 3-acetoxy beta-lactam + H2O stereospecificity in hydrolysis of 3-acetoxy beta-lactam Priestia megaterium ? - ? 390723 3.1.1.3 4-methylumbelliferyl butyrate + H2O - Priestia megaterium 4-methylumbelliferol + butyrate - ? 363849 3.1.1.3 4-nitrophenyl butyrate + H2O high activity Priestia megaterium 4-nitrophenol + butyrate - ? 115376 3.1.1.3 4-nitrophenyl caprate + H2O - Priestia megaterium 4-nitrophenol + caprate - ? 364083 3.1.1.3 4-nitrophenyl caproate + H2O - Priestia megaterium 4-nitrophenol + caproate - ? 363887 3.1.1.3 4-nitrophenyl caprylate + H2O - Priestia megaterium 4-nitrophenol + caprylate - ? 363889 3.1.1.3 4-nitrophenyl laurate + H2O - Priestia megaterium 4-nitrophenol + laurate - ? 363891 3.1.1.3 4-nitrophenyl valerate + H2O - Priestia megaterium 4-nitrophenol + valerate - ? 363886 3.1.1.3 additional information the recombinant enzyme displays high activity on short to medium chain length substrates, and poor activity on C18 substrates, overview, poor activity with 4-nitrophenyl palmitate, 4-nitrophenyl stearate, and 4-methylumbelliferyl oleate Priestia megaterium ? - ? 89 3.1.1.3 olive oil + H2O - Priestia megaterium ? - ? 364063 3.1.1.3 tributyrin + H2O - Priestia megaterium dibutyrin + butyrate - ? 363949 3.1.1.3 triolein + H2O - Priestia megaterium diolein + oleate - ? 363933 3.1.1.3 3-acetoxy beta-lactam + H2O stereospecificity in hydrolysis of 3-acetoxy beta-lactam Priestia megaterium AKG-1 ? - ? 390723 3.1.1.3 4-nitrophenyl laurate + H2O - Priestia megaterium AKG-1 4-nitrophenol + laurate - ? 363891 3.1.1.3 tributyrin + H2O - Priestia megaterium AKG-1 dibutyrin + butyrate - ? 363949 3.1.1.3 4-methylumbelliferyl butyrate + H2O - Priestia megaterium ATCC 9885 4-methylumbelliferol + butyrate - ? 363849 3.1.1.3 4-nitrophenyl butyrate + H2O high activity Priestia megaterium ATCC 9885 4-nitrophenol + butyrate - ? 115376 3.1.1.3 4-nitrophenyl caproate + H2O - Priestia megaterium ATCC 9885 4-nitrophenol + caproate - ? 363887 3.1.1.3 4-nitrophenyl valerate + H2O - Priestia megaterium ATCC 9885 4-nitrophenol + valerate - ? 363886 3.1.1.3 additional information the recombinant enzyme displays high activity on short to medium chain length substrates, and poor activity on C18 substrates, overview, poor activity with 4-nitrophenyl palmitate, 4-nitrophenyl stearate, and 4-methylumbelliferyl oleate Priestia megaterium ATCC 9885 ? - ? 89 3.1.1.3 tributyrin + H2O - Proteus vulgaris dibutyrin + butyrate - ? 363949 3.1.1.3 4-nitrophenyl acetate + H2O - Pseudoalteromonas haloplanktis 4-nitrophenol + acetate - ? 12514 3.1.1.3 4-nitrophenyl caproate + H2O best substrate Pseudoalteromonas haloplanktis 4-nitrophenol + caproate - ? 363887 3.1.1.3 4-nitrophenyl hexanoate + H2O - Pseudoalteromonas haloplanktis 4-nitrophenol + hexanoate - ? 115379 3.1.1.3 4-nitrophenyl octanoate + H2O - Pseudoalteromonas haloplanktis 4-nitrophenol + octanoate - ? 115380 3.1.1.3 4-nitrophenyl pentanoate + H2O - Pseudoalteromonas haloplanktis 4-nitrophenol + pentanoate - ? 115378 3.1.1.3 tricaprylin + H2O - Pseudoalteromonas haloplanktis ? - ? 64400 3.1.1.3 triolein + H2O - Pseudoalteromonas haloplanktis ? - ? 64454 3.1.1.3 4-nitrophenyl acetate + H2O - Pseudoalteromonas haloplanktis TAC 125 4-nitrophenol + acetate - ? 12514 3.1.1.3 4-nitrophenyl hexanoate + H2O - Pseudoalteromonas haloplanktis TAC 125 4-nitrophenol + hexanoate - ? 115379 3.1.1.3 4-nitrophenyl pentanoate + H2O - Pseudoalteromonas haloplanktis TAC 125 4-nitrophenol + pentanoate - ? 115378 3.1.1.3 tricaprylin + H2O - Pseudoalteromonas haloplanktis TAC 125 ? - ? 64400 3.1.1.3 triolein + H2O - Pseudoalteromonas haloplanktis TAC 125 ? - ? 64454 3.1.1.3 1,2-dilauryl-rac-glycero-3-glutaric acid resorufinester + H2O - Pseudomonas aeruginosa ? - ? 380828 3.1.1.3 2,3-dimercaptopropan-1-ol tributyrate + H2O - Pseudomonas aeruginosa ? - ? 380892 3.1.1.3 2,3-dimercaptopropan-1-ol tributyrate + H2O liberation of thiol groups by hydrolysis Pseudomonas aeruginosa ? - ? 380892 3.1.1.3 2-methyldecanoic acid 4-nitrophenyl ester + H2O model substrate, enantioselectivity in the asymmetric hydrolysis with preference for the S-enantiomer Pseudomonas aeruginosa (S)-2-methyldecanoate + 4-nitrophenol + (R)-2-methyldecanoic acid 4-nitrophenyl ester - ? 399446 3.1.1.3 3 triolein + 3 H2O - Pseudomonas aeruginosa 1,3-diolein + 1,2-diolein + 2,3-diolein + 3 oleate shows random positional specificity for triolein hydrolysis ? 402049 3.1.1.3 4-nitrophenyl 2-methyldecanoate + H2O kinetic resolution of enantioselectivity, overview Pseudomonas aeruginosa ? - ? 390892 3.1.1.3 4-nitrophenyl 4-cyclohexyl-2-methylbuta-2,3-dienoate + H2O reaction via R-tetrahedral intermediate and S-tetrahedral intermediate, overview, the wild-type lipase and the mutant L162F show preference for the (+)-enantiomer, the mutant shows high enantioselectivity, kinetic resolution of enantioselectivity, overview Pseudomonas aeruginosa ? - ? 390903 3.1.1.3 4-nitrophenyl acetate + H2O - Pseudomonas aeruginosa 4-nitrophenol + acetate - ? 12514 3.1.1.3 4-nitrophenyl butyrate + H2O - Pseudomonas aeruginosa 4-nitrophenol + butyrate - ? 115376 3.1.1.3 4-nitrophenyl caprate + H2O - Pseudomonas aeruginosa 4-nitrophenol + caprate - ? 364083 3.1.1.3 4-nitrophenyl laurate + H2O purified enzyme of strain F-111 shows preference for Pseudomonas aeruginosa 4-nitrophenol + laurate - ? 363891 3.1.1.3 4-nitrophenyl laurate + H2O high activity in a water-restricted environment containing a water content of 0.5-1% w/w Pseudomonas aeruginosa 4-nitrophenol + laurate - ? 363891 3.1.1.3 4-nitrophenyl myristate + H2O - Pseudomonas aeruginosa 4-nitrophenol + myristate - ? 364082 3.1.1.3 4-nitrophenyl myristate + H2O purified enzyme of strain F-111 shows preference for Pseudomonas aeruginosa 4-nitrophenol + myristate - ? 364082 3.1.1.3 4-nitrophenyl palmitate + H2O - Pseudomonas aeruginosa 4-nitrophenol + palmitate - ? 363890 3.1.1.3 4-nitrophenyl palmitate + H2O the immobilized enzyme PAL PCMC works as better catalyst for hydrolysis of pNPP in n-heptane medium Pseudomonas aeruginosa palmitate + 4-nitrophenol - ? 399694 3.1.1.3 4-nitrophenyl stearate + H2O - Pseudomonas aeruginosa 4-nitrophenol + stearate - ? 364135 3.1.1.3 acylglycerol + H2O - Pseudomonas aeruginosa glycerol + a carboxylate - ? 381341 3.1.1.3 butyl butyrate + H2O - Pseudomonas aeruginosa butane + butyrate - ? 400581 3.1.1.3 castor oil + H2O - Pseudomonas aeruginosa ? - ? 364069 3.1.1.3 diacylglycerol + H2O - Pseudomonas aeruginosa acylglycerol + a carboxylate - ? 381795 3.1.1.3 ethyl butyrate + H2O - Pseudomonas aeruginosa ethane + butyrate - ? 400922 3.1.1.3 methyl oleate + H2O acylglycerol lipase activity, EC 3.1.1.23 Pseudomonas aeruginosa methanol + oleate - ? 364075 3.1.1.3 additional information the extracellular enzyme inhibits the chemotaxis and chemiluminescence of monocytes, but has little effect on neutrophils, in human peripheral blood contributing to pathogenesis, overview Pseudomonas aeruginosa ? - ? 89 3.1.1.3 additional information substrate specificity of the extracellular enzyme, the enzyme shows high activity Pseudomonas aeruginosa ? - ? 89 3.1.1.3 additional information the enzyme also shows 8fold lower esterase activity with 4-nitrophenyl acetate and Tween 80 as substrates Pseudomonas aeruginosa ? - ? 89 3.1.1.3 additional information the enzyme is unspecific, but prefers substrates with shorter side chains, exo-enzyme Pseudomonas aeruginosa ? - ? 89 3.1.1.3 additional information the extracellular enzyme form is an exo-enzyme Pseudomonas aeruginosa ? - ? 89 3.1.1.3 additional information double-lid structure movements are involved in the enzyme function, Phe214 and Ala217 play important roles in lid movement, lid closure is driven by hydrophobic interactions, overview Pseudomonas aeruginosa ? - ? 89 3.1.1.3 additional information lipases catalyze the hydrolysis of neutral lipids in biological systems performing three reactions: hydrolysis of ester in aqueous solutions, esterification in organic solvents, and transesterification between ester and acyl group donor with a wide substrate specificiy, e.g. lipids, sugars, alcohols, acids, and esters Pseudomonas aeruginosa ? - ? 89 3.1.1.3 oleoyl 2-naphthyl ester + H2O - Pseudomonas aeruginosa oleic acid + 2-naphthol - ? 393051 3.1.1.3 oleoyl 2-naphthylamide + H2O - Pseudomonas aeruginosa oleic acid + 2-naphthylamine - ? 393052 3.1.1.3 soybean oil + H2O concentrated soybean oil, high activity in a water-restricted environment containing a water content of 0.5-1% w/w Pseudomonas aeruginosa ? - ? 364064 3.1.1.3 trans-3-(4-methoxyphenyl) glycidic acid methyl ester + H2O i.e. (+-)-methyl trans-3(4-methoxyphenyl) glycidate, the substrate is a key intermediate in the synthesis of cardiovascular drug, diltiazem Pseudomonas aeruginosa ? - ? 393688 3.1.1.3 triacetin + H2O - Pseudomonas aeruginosa diacetin + acetate - ? 364163 3.1.1.3 triacylglycerol + H2O - Pseudomonas aeruginosa diacylglycerol + a carboxylate - ? 360693 3.1.1.3 triacylglycerol + H2O the enzyme acts on triacylglycerols with medium fatty acid chain length, substrate specificity, overview Pseudomonas aeruginosa diacylglycerol + a carboxylate - ? 360693 3.1.1.3 tributyrin + H2O - Pseudomonas aeruginosa dibutyrin + butyrate - ? 363949 3.1.1.3 tricaprin + H2O high activity Pseudomonas aeruginosa dicaprin + caprate - ? 364183 3.1.1.3 tricaproin + H2O high activity Pseudomonas aeruginosa dicaproin + caproate - ? 364147 3.1.1.3 tricaprylin + H2O best substrate Pseudomonas aeruginosa dicaprylin + caprylate - ? 364128 3.1.1.3 trimyristin + H2O - Pseudomonas aeruginosa dimyristin + myristate - ? 364132 3.1.1.3 triolein + H2O - Pseudomonas aeruginosa diolein + oleate - ? 363933 3.1.1.3 triolein + H2O olive oil with predominantly triolein, regiospecificity for the 1- and 3-oleoyl residues Pseudomonas aeruginosa diolein + oleate - ? 363933 3.1.1.3 trioleoylglycerol + H2O - Pseudomonas aeruginosa oleic acid + ? - ? 64389 3.1.1.3 tripropionin + H2O - Pseudomonas aeruginosa dipropionin + propionate - ? 364079 3.1.1.3 acylglycerol + H2O - Pseudomonas aeruginosa ATCC 10145 glycerol + a carboxylate - ? 381341 3.1.1.3 diacylglycerol + H2O - Pseudomonas aeruginosa ATCC 10145 acylglycerol + a carboxylate - ? 381795 3.1.1.3 additional information the enzyme is unspecific, but prefers substrates with shorter side chains, exo-enzyme Pseudomonas aeruginosa ATCC 10145 ? - ? 89 3.1.1.3 triacylglycerol + H2O - Pseudomonas aeruginosa ATCC 10145 diacylglycerol + a carboxylate - ? 360693 3.1.1.3 tributyrin + H2O - Pseudomonas aeruginosa ATCC 10145 dibutyrin + butyrate - ? 363949 3.1.1.3 additional information the enzyme also shows 8fold lower esterase activity with 4-nitrophenyl acetate and Tween 80 as substrates Pseudomonas aeruginosa EF2 ? - ? 89 3.1.1.3 triolein + H2O olive oil with predominantly triolein, regiospecificity for the 1- and 3-oleoyl residues Pseudomonas aeruginosa EF2 diolein + oleate - ? 363933 3.1.1.3 2,3-dimercaptopropan-1-ol tributyrate + H2O liberation of thiol groups by hydrolysis Pseudomonas aeruginosa LST-03 ? - ? 380892 3.1.1.3 2,3-dimercaptopropan-1-ol tributyrate + H2O - Pseudomonas aeruginosa LST-03 ? - ? 380892 3.1.1.3 tributyrin + H2O - Pseudomonas aeruginosa LST-03 dibutyrin + butyrate - ? 363949 3.1.1.3 4-nitrophenyl palmitate + H2O - Pseudomonas aeruginosa MTCC 5113 4-nitrophenol + palmitate - ? 363890 3.1.1.3 trans-3-(4-methoxyphenyl) glycidic acid methyl ester + H2O i.e. (+-)-methyl trans-3(4-methoxyphenyl) glycidate, the substrate is a key intermediate in the synthesis of cardiovascular drug, diltiazem Pseudomonas aeruginosa MTCC 5113 ? - ? 393688 3.1.1.3 additional information lipases catalyze the hydrolysis of neutral lipids in biological systems performing three reactions: hydrolysis of ester in aqueous solutions, esterification in organic solvents, and transesterification between ester and acyl group donor with a wide substrate specificiy, e.g. lipids, sugars, alcohols, acids, and esters Pseudomonas aeruginosa MTCC-2297 ? - ? 89 3.1.1.3 additional information the extracellular enzyme inhibits the chemotaxis and chemiluminescence of monocytes, but has little effect on neutrophils, in human peripheral blood contributing to pathogenesis, overview Pseudomonas aeruginosa PAC 1R ? - ? 89 3.1.1.3 4-nitrophenyl palmitate + H2O - Pseudomonas aeruginosa PAC1R 4-nitrophenol + palmitate - ? 363890 3.1.1.3 triacetin + H2O - Pseudomonas aeruginosa PAC1R diacetin + acetate - ? 364163 3.1.1.3 tripropionin + H2O - Pseudomonas aeruginosa PAC1R dipropionin + propionate - ? 364079 3.1.1.3 additional information the extracellular enzyme form is an exo-enzyme Pseudomonas aeruginosa PAO 2302 ? - ? 89 3.1.1.3 trimyristin + H2O - Pseudomonas aeruginosa PAO 2302 dimyristin + myristate - ? 364132 3.1.1.3 4-nitrophenyl palmitate + H2O - Pseudomonas aeruginosa Ps-x 4-nitrophenol + palmitate - ? 363890 3.1.1.3 4-nitrophenyl acetate + H2O - Pseudomonas aeruginosa PseA 4-nitrophenol + acetate - ? 12514 3.1.1.3 4-nitrophenyl butyrate + H2O - Pseudomonas aeruginosa PseA 4-nitrophenol + butyrate - ? 115376 3.1.1.3 4-nitrophenyl caprate + H2O - Pseudomonas aeruginosa PseA 4-nitrophenol + caprate - ? 364083 3.1.1.3 4-nitrophenyl myristate + H2O - Pseudomonas aeruginosa PseA 4-nitrophenol + myristate - ? 364082 3.1.1.3 4-nitrophenyl palmitate + H2O - Pseudomonas aeruginosa PseA 4-nitrophenol + palmitate - ? 363890 3.1.1.3 4-nitrophenyl laurate + H2O high activity in a water-restricted environment containing a water content of 0.5-1% w/w Pseudomonas aeruginosa YS-7 4-nitrophenol + laurate - ? 363891 3.1.1.3 soybean oil + H2O concentrated soybean oil, high activity in a water-restricted environment containing a water content of 0.5-1% w/w Pseudomonas aeruginosa YS-7 ? - ? 364064 3.1.1.3 1,2-dilauryl-rac-glycero-3-glutaric acid resorufinester + H2O - Pseudomonas alcaligenes ? - ? 380828 3.1.1.3 4-nitrophenyl palmitate + H2O - Pseudomonas alcaligenes 4-nitrophenol + palmitate - ? 363890 3.1.1.3 additional information substrate specificity of the extracellular enzyme Pseudomonas alcaligenes ? - ? 89 3.1.1.3 tributyrin + H2O - Pseudomonas alcaligenes dibutyrin + butyrate - ? 363949 3.1.1.3 triolein + H2O - Pseudomonas alcaligenes diolein + oleate - ? 363933 3.1.1.3 (+-)-2-hydroxy-4-phenylbutyric acid ethyl ester + H2O enantioselectivity of the immobilized enzyme in this reaction is increased 40fold by presence of 0.1% CTAB Pseudomonas fluorescens ? - ? 390346 3.1.1.3 (3aR,6aS)-2-oxo-3,3a,4,6a-tetrahydro-2H-cyclopenta[b]furan-3-yl acetate + H2O - Pseudomonas fluorescens (3S,3aR,6aS)-2-oxo-3,3a,4,6a-tetrahydro-2H-cyclopenta[b]furan-3-yl acetate + (3R,3aS,6aS)-3-hydroxy-3,3a,4,6a-tetrahydro-2H-cyclopenta[b]furan-2-one + acetate - ? 375383 3.1.1.3 (5R,6R)-6-hydroxy-5,6-dihydro-1,10-phenanthrolin-5-yl acetate + vinyl acetate - Pseudomonas fluorescens (5S,6S)-6-hydroxy-5,6-dihydro-1,10-phenanthrolin-5-yl acetate + (5R,6R)-5,6-dihydro-1,10-phenanthroline-5,6-diyl diacetate + ethenol - ? 399165 3.1.1.3 1,2-dilauryl-rac-glycero-3-glutaric acid resorufinester + H2O - Pseudomonas fluorescens ? - ? 380828 3.1.1.3 4-nitrophenyl caprate + H2O - Pseudomonas fluorescens 4-nitrophenol + caprate - ? 364083 3.1.1.3 4-nitrophenyl palmitate + H2O - Pseudomonas fluorescens 4-nitrophenol + palmitate - ? 363890 3.1.1.3 6-azido-5,6-dihydro-1,10-phenanthrolin-5-ol + vinyl acetate - Pseudomonas fluorescens (5S,6S)-6-azido-5,6-dihydro-1,10-phenanthrolin-5-ol + (5R,6R)-6-azido-5,6-dihydro-1,10-phenanthrolin-5-yl acetate + ethenol - ? 399164 3.1.1.3 high linoleic sunflower oil + H2O - Pseudomonas fluorescens ? - ? 401143 3.1.1.3 high oleic sunflower oil + H2O - Pseudomonas fluorescens ? - ? 401146 3.1.1.3 methyl butyrate + H2O - Pseudomonas fluorescens methanol + butyrate - ? 115393 3.1.1.3 methyl caprate + H2O best substrate Pseudomonas fluorescens methanol + caprate - ? 364186 3.1.1.3 methyl caproate + H2O - Pseudomonas fluorescens methanol + caproate - ? 364184 3.1.1.3 methyl caprylate + H2O - Pseudomonas fluorescens methanol + caprylate - ? 364185 3.1.1.3 methyl icosanoate + H2O - Pseudomonas fluorescens methanol + icosanoate - ? 364192 3.1.1.3 methyl laurate + H2O - Pseudomonas fluorescens methanol + laurate - ? 364077 3.1.1.3 methyl linoleate + H2O - Pseudomonas fluorescens methanol + linoleate - ? 364188 3.1.1.3 methyl linolenate + H2O - Pseudomonas fluorescens methanol + linolenate - ? 364189 3.1.1.3 methyl myristate + H2O - Pseudomonas fluorescens methanol + myristate - ? 364187 3.1.1.3 methyl oleate + H2O - Pseudomonas fluorescens methanol + oleate - ? 364075 3.1.1.3 methyl palmitate + H2O - Pseudomonas fluorescens methanol + palmitate - ? 364076 3.1.1.3 methyl propionate + H2O - Pseudomonas fluorescens methanol + propionate - ? 115453 3.1.1.3 methyl stearate + H2O - Pseudomonas fluorescens methanol + stearate - ? 364078 3.1.1.3 additional information substrate specificity overview, enzyme splits polyunsaturated fatty acid ester bonds, the enzyme preferably acts on short- to middle-chain length fatty acid simple methyl esters and triglycerides Pseudomonas fluorescens ? - ? 89 3.1.1.3 additional information the enzyme is responsible for the spoilage of milk Pseudomonas fluorescens ? - ? 89 3.1.1.3 additional information substrate specificity of the recombinant extracellular enzyme overexpressed in Escherichia coli Pseudomonas fluorescens ? - ? 89 3.1.1.3 additional information LipB68 performs the enantioselective acylation of racemic alcohols, e.g. toluene, and effectively catalyzes the transesterification of both alpha-phenylethanol and alpha-phenylpropanol at 20°C with high enantioselectivity, overview Pseudomonas fluorescens ? - ? 89 3.1.1.3 olive oil + H2O emulsion Pseudomonas fluorescens ? - ? 364063 3.1.1.3 triacetin + H2O low activity Pseudomonas fluorescens diacetin + acetate - ? 364163 3.1.1.3 tributyrin + H2O - Pseudomonas fluorescens dibutyrin + butyrate - ? 363949 3.1.1.3 tributyrin + H2O best substrate Pseudomonas fluorescens dibutyrin + butyrate - ? 363949 3.1.1.3 tricaprin + H2O best substrate Pseudomonas fluorescens dicaprin + caprate - ? 364183 3.1.1.3 tricaproin + H2O best substrate Pseudomonas fluorescens dicaproin + caproate - ? 364147 3.1.1.3 tricaprylin + H2O best substrate Pseudomonas fluorescens dicaprylin + caprylate - ? 364128 3.1.1.3 trihexanoin + H2O - Pseudomonas fluorescens hexanoic acid + ? - ? 64397 3.1.1.3 trilaurin + H2O - Pseudomonas fluorescens dilaurin + laurate - ? 364081 3.1.1.3 trimyristin + H2O low activity Pseudomonas fluorescens dimyristin + myristate - ? 364132 3.1.1.3 triolein + H2O - Pseudomonas fluorescens diolein + oleate - ? 363933 3.1.1.3 triolein + H2O cleaves mainly 1,3-ester bonds, and to a lesser extent the 2-position ester bond Pseudomonas fluorescens diolein + oleate - ? 363933 3.1.1.3 tripalmitin + H2O - Pseudomonas fluorescens palmitic acid + ? - ? 64402 3.1.1.3 tripalmitin + H2O low activity Pseudomonas fluorescens dipalmitin + palmitate - ? 364080 3.1.1.3 tristearin + H2O - Pseudomonas fluorescens distearin + stearate - ? 364134 3.1.1.3 tributyrin + H2O - Pseudomonas fluorescens B52 dibutyrin + butyrate - ? 363949 3.1.1.3 4-nitrophenyl caprate + H2O - Pseudomonas fluorescens B68 4-nitrophenol + caprate - ? 364083 3.1.1.3 additional information LipB68 performs the enantioselective acylation of racemic alcohols, e.g. toluene, and effectively catalyzes the transesterification of both alpha-phenylethanol and alpha-phenylpropanol at 20°C with high enantioselectivity, overview Pseudomonas fluorescens B68 ? - ? 89 3.1.1.3 1,2-dilauryl-rac-glycero-3-glutaric acid resorufinester + H2O - Pseudomonas fluorescens GK13 ? - ? 380828 3.1.1.3 4-nitrophenyl palmitate + H2O - Pseudomonas fluorescens GK13 4-nitrophenol + palmitate - ? 363890 3.1.1.3 additional information substrate specificity of the recombinant extracellular enzyme overexpressed in Escherichia coli Pseudomonas fluorescens GK13 ? - ? 89 3.1.1.3 triolein + H2O - Pseudomonas fluorescens GK13 diolein + oleate - ? 363933 3.1.1.3 methyl butyrate + H2O - Pseudomonas fluorescens HU380 methanol + butyrate - ? 115393 3.1.1.3 methyl propionate + H2O - Pseudomonas fluorescens HU380 methanol + propionate - ? 115453 3.1.1.3 additional information substrate specificity overview, enzyme splits polyunsaturated fatty acid ester bonds, the enzyme preferably acts on short- to middle-chain length fatty acid simple methyl esters and triglycerides Pseudomonas fluorescens HU380 ? - ? 89 3.1.1.3 tributyrin + H2O best substrate Pseudomonas fluorescens HU380 dibutyrin + butyrate - ? 363949 3.1.1.3 triolein + H2O cleaves mainly 1,3-ester bonds, and to a lesser extent the 2-position ester bond Pseudomonas fluorescens HU380 diolein + oleate - ? 363933 3.1.1.3 additional information the enzyme is responsible for the spoilage of milk Pseudomonas fluorescens SIK W1 ? - ? 89 3.1.1.3 glyceryl trioctanoate + H2O - Pseudomonas fragi glycerol + octanoate - ? 401073 3.1.1.3 additional information substrate specificity, the enzyme prefers triacylglycerides with short fatty acid chains Pseudomonas fragi ? - ? 89 3.1.1.3 tributyrin + H2O preferred substrate Pseudomonas fragi dibutyrin + butyrate - ? 363949 3.1.1.3 tricaprylin + H2O - Pseudomonas fragi dicaprylin + caprylate - ? 364128 3.1.1.3 trilaurin + H2O - Pseudomonas fragi dilaurin + laurate - ? 364081 3.1.1.3 trimyristin + H2O - Pseudomonas fragi dimyristin + myristate - ? 364132 3.1.1.3 triolein + H2O low activity Pseudomonas fragi diolein + oleate - ? 363933 3.1.1.3 tripalmitin + H2O - Pseudomonas fragi dipalmitin + palmitate - ? 364080 3.1.1.3 tristearin + H2O low activity Pseudomonas fragi distearin + stearate - ? 364134 3.1.1.3 additional information substrate specificity, the enzyme prefers triacylglycerides with short fatty acid chains Pseudomonas fragi IFO 3458 ? - ? 89 3.1.1.3 tributyrin + H2O preferred substrate Pseudomonas fragi IFO 3458 dibutyrin + butyrate - ? 363949 3.1.1.3 trilaurin + H2O - Pseudomonas fragi IFO 3458 dilaurin + laurate - ? 364081 3.1.1.3 triolein + H2O low activity Pseudomonas fragi IFO 3458 diolein + oleate - ? 363933 3.1.1.3 tripalmitin + H2O - Pseudomonas fragi IFO 3458 dipalmitin + palmitate - ? 364080 3.1.1.3 amyl acetate + H2O 7% activity compared to olive oil Pseudomonas mendocina ? - ? 416568 3.1.1.3 amyl butyrate + H2O 550% activity compared to olive oil Pseudomonas mendocina ? - ? 416569 3.1.1.3 amyl propionate + H2O 40% activity compared to olive oil Pseudomonas mendocina ? - ? 416570 3.1.1.3 benzyl butyrate + H2O highest activity, 1120% activity compared to olive oil Pseudomonas mendocina ? - ? 416677 3.1.1.3 benzyl propionate + H2O 950% activity compared to olive oil Pseudomonas mendocina benzyl alcohol + propionate - ? 115409 3.1.1.3 benzyl salicylate + H2O 100% activity compared to olive oil Pseudomonas mendocina ? - ? 416680 3.1.1.3 ethyl butyrate + H2O 245% activity compared to olive oil Pseudomonas mendocina ? - ? 416985 3.1.1.3 additional information enzyme shows a preference for long-chain fatty acid triacylglyceride substrates and natural oils like olive oil, soybean oil, mustard oil, coconut oil, and best almond oil, substrate specificty overview Pseudomonas mendocina ? - ? 89 3.1.1.3 additional information Pseudomonas mendocina PK-12CS is able to grow on olive oil, soybean oil, tributyrin and triolein as sole carbon and energysource Pseudomonas mendocina ? - ? 89 3.1.1.3 additional information the enzyme also uses olive oil (100% activity), mustard oil (96% activity), soybean oil (88.8% activity), palm oil (73% activity), and groundnut oil (92% activity) as substrates Pseudomonas mendocina ? - ? 89 3.1.1.3 additional information the lipase possesses 1,3-positional specificity Pseudomonas mendocina ? - ? 89 3.1.1.3 triacetin + H2O 368% activity compared to olive oil Pseudomonas mendocina diacetin + acetate - ? 364163 3.1.1.3 tributyrin + H2O - Pseudomonas mendocina dibutyrin + butyrate - ? 363949 3.1.1.3 tributyrin + H2O 744% activity compared to olive oil Pseudomonas mendocina dibutyrin + butyrate - ? 363949 3.1.1.3 tricaprylin + H2O 89% activity compared to olive oil Pseudomonas mendocina dicaprylin + caprylate - ? 364128 3.1.1.3 trilinolin + H2O - Pseudomonas mendocina dilinolin + linolate - ? 364177 3.1.1.3 triolein + H2O - Pseudomonas mendocina diolein + oleate - ? 363933 3.1.1.3 triolein + H2O 110% activity compared to olive oil Pseudomonas mendocina diolein + oleate - ? 363933 3.1.1.3 tripalmitin + H2O - Pseudomonas mendocina dipalmitin + palmitate - ? 364080 3.1.1.3 benzyl propionate + H2O 950% activity compared to olive oil Pseudomonas mendocina M-37 benzyl alcohol + propionate - ? 115409 3.1.1.3 additional information the enzyme also uses olive oil (100% activity), mustard oil (96% activity), soybean oil (88.8% activity), palm oil (73% activity), and groundnut oil (92% activity) as substrates Pseudomonas mendocina M-37 ? - ? 89 3.1.1.3 additional information the lipase possesses 1,3-positional specificity Pseudomonas mendocina M-37 ? - ? 89 3.1.1.3 tributyrin + H2O 744% activity compared to olive oil Pseudomonas mendocina M-37 dibutyrin + butyrate - ? 363949 3.1.1.3 triolein + H2O 110% activity compared to olive oil Pseudomonas mendocina M-37 diolein + oleate - ? 363933 3.1.1.3 additional information enzyme shows a preference for long-chain fatty acid triacylglyceride substrates and natural oils like olive oil, soybean oil, mustard oil, coconut oil, and best almond oil, substrate specificty overview Pseudomonas mendocina PK-12CS ? - ? 89 3.1.1.3 additional information Pseudomonas mendocina PK-12CS is able to grow on olive oil, soybean oil, tributyrin and triolein as sole carbon and energysource Pseudomonas mendocina PK-12CS ? - ? 89 3.1.1.3 tributyrin + H2O - Pseudomonas mendocina PK-12CS dibutyrin + butyrate - ? 363949 3.1.1.3 triolein + H2O - Pseudomonas mendocina PK-12CS diolein + oleate - ? 363933 3.1.1.3 tripalmitin + H2O - Pseudomonas mendocina PK-12CS dipalmitin + palmitate - ? 364080 3.1.1.3 tributyrin + H2O - Pseudomonas oleovorans dibutyrin + butyrate - ? 363949 3.1.1.3 4-nitrophenyl acetate + H2O - Pseudomonas sp. 4-nitrophenol + acetate - ? 12514 3.1.1.3 4-nitrophenyl butyrate + H2O low activity Pseudomonas sp. 4-nitrophenol + butyrate - ? 115376 3.1.1.3 4-nitrophenyl caprate + H2O - Pseudomonas sp. 4-nitrophenol + caprate - ? 364083 3.1.1.3 4-nitrophenyl caprate + H2O high activity Pseudomonas sp. 4-nitrophenol + caprate - ? 364083 3.1.1.3 4-nitrophenyl caproate + H2O high activity Pseudomonas sp. 4-nitrophenol + caproate - ? 363887 3.1.1.3 4-nitrophenyl caprylate + H2O high activity Pseudomonas sp. 4-nitrophenol + caprylate - ? 363889 3.1.1.3 4-nitrophenyl laurate + H2O - Pseudomonas sp. 4-nitrophenol + laurate - ? 363891 3.1.1.3 4-nitrophenyl myristate + H2O - Pseudomonas sp. 4-nitrophenol + myristate - ? 364082 3.1.1.3 4-nitrophenyl myristate + H2O high activity Pseudomonas sp. 4-nitrophenol + myristate - ? 364082 3.1.1.3 4-nitrophenyl palmitate + H2O best substrate Pseudomonas sp. 4-nitrophenol + palmitate - ? 363890 3.1.1.3 4-nitrophenyl palmitate + H2O high activity Pseudomonas sp. 4-nitrophenol + palmitate - ? 363890 3.1.1.3 4-nitrophenyl stearate + H2O - Pseudomonas sp. 4-nitrophenol + stearate - ? 364135 3.1.1.3 castor oil + H2O major acyl group is ricinoleic acid Pseudomonas sp. ? - ? 364069 3.1.1.3 coconut oil + H2O major acyl group is palmitic acid Pseudomonas sp. ? - ? 364067 3.1.1.3 corn oil + H2O major acyl groups are oleic acid and linoleic acid Pseudomonas sp. ? - ? 364066 3.1.1.3 glyceryl triacetate + H2O - Pseudomonas sp. glyceryl diacetate + acetic acid - ? 364084 3.1.1.3 glyceryl tributyrate + H2O - Pseudomonas sp. glyceryl dibutyrate + butyric acid - ? 364085 3.1.1.3 glyceryl tricaproate + H2O - Pseudomonas sp. glyceryl dicaproate + caproic acid - ? 364087 3.1.1.3 glyceryl tricaprylate + H2O - Pseudomonas sp. glyceryl dicaprylate + caprylic acid - ? 364086 3.1.1.3 groundnut oil + H2O major acyl group is oleic acid Pseudomonas sp. ? - ? 364068 3.1.1.3 linseed oil + H2O major acyl groups are oleic and linoleic acid Pseudomonas sp. ? - ? 364071 3.1.1.3 additional information enantioselective transesterification of 2-phenoxy-1-propanol Pseudomonas sp. ? - ? 89 3.1.1.3 additional information enzyme shows a preference for long-chain fatty acid triacylglyceride substrates Pseudomonas sp. ? - ? 89 3.1.1.3 additional information olive oil is utilized as substrate with high activity Pseudomonas sp. ? - ? 89 3.1.1.3 mustard oil + H2O major acyl groups are erucic and oleic acid Pseudomonas sp. ? - ? 364072 3.1.1.3 neem oil + H2O major acyl groups are palmitic, stearic and oleic acid Pseudomonas sp. ? - ? 364070 3.1.1.3 olive oil + H2O - Pseudomonas sp. ? - ? 364063 3.1.1.3 olive oil + H2O best substrate, major acyl group is oleic acid Pseudomonas sp. ? - ? 364063 3.1.1.3 p-nitrophenyl caprate + H2O highest activity with p-nitrophenyl caprate Pseudomonas sp. p-nitrophenol + caprate - ? 401582 3.1.1.3 soybean oil + H2O major acyl groups are oleic acid and linoleic acid Pseudomonas sp. ? - ? 364064 3.1.1.3 sunflower oil + H2O major acyl group is linoleic acid Pseudomonas sp. ? - ? 364065 3.1.1.3 tributyrin + H2O - Pseudomonas sp. dibutyrin + butyrate - ? 363949 3.1.1.3 p-nitrophenyl caprate + H2O highest activity with p-nitrophenyl caprate Pseudomonas sp. B11-1 p-nitrophenol + caprate - ? 401582 3.1.1.3 p-nitrophenyl caprate + H2O highest activity with p-nitrophenyl caprate Pseudomonas sp. KB700A p-nitrophenol + caprate - ? 401582 3.1.1.3 additional information enantioselective transesterification of 2-phenoxy-1-propanol Pseudomonas sp. KM1-56 ? - ? 89 3.1.1.3 tributyrin + H2O - Pseudomonas sp. KM1-56 dibutyrin + butyrate - ? 363949 3.1.1.3 4-nitrophenyl acetate + H2O - Pseudomonas sp. MIS38 4-nitrophenol + acetate - ? 12514 3.1.1.3 4-nitrophenyl butyrate + H2O low activity Pseudomonas sp. MIS38 4-nitrophenol + butyrate - ? 115376 3.1.1.3 4-nitrophenyl caproate + H2O high activity Pseudomonas sp. MIS38 4-nitrophenol + caproate - ? 363887 3.1.1.3 4-nitrophenyl caprylate + H2O high activity Pseudomonas sp. MIS38 4-nitrophenol + caprylate - ? 363889 3.1.1.3 4-nitrophenyl laurate + H2O - Pseudomonas sp. MIS38 4-nitrophenol + laurate - ? 363891 3.1.1.3 additional information olive oil is utilized as substrate with high activity Pseudomonas sp. MIS38 ? - ? 89 3.1.1.3 olive oil + H2O - Pseudomonas sp. MIS38 ? - ? 364063 3.1.1.3 4-nitrophenyl caprate + H2O - Pseudomonas sp. SW-3 4-nitrophenol + caprate - ? 364083 3.1.1.3 4-nitrophenyl laurate + H2O - Pseudomonas sp. SW-3 4-nitrophenol + laurate - ? 363891 3.1.1.3 4-nitrophenyl myristate + H2O - Pseudomonas sp. SW-3 4-nitrophenol + myristate - ? 364082 3.1.1.3 4-nitrophenyl palmitate + H2O best substrate Pseudomonas sp. SW-3 4-nitrophenol + palmitate - ? 363890 3.1.1.3 additional information enzyme shows a preference for long-chain fatty acid triacylglyceride substrates Pseudomonas sp. SW-3 ? - ? 89 3.1.1.3 4-nitrophenyl acetate + H2O - Psychrobacter sp. 4-nitrophenol + acetate - ? 12514 3.1.1.3 4-nitrophenyl butyrate + H2O - Psychrobacter sp. 4-nitrophenol + butyrate - ? 115376 3.1.1.3 4-nitrophenyl caprate + H2O high activity Psychrobacter sp. 4-nitrophenol + caprate - ? 364083 3.1.1.3 4-nitrophenyl caproate + H2O best substrate Psychrobacter sp. 4-nitrophenol + caproate - ? 363887 3.1.1.3 4-nitrophenyl laurate + H2O - Psychrobacter sp. 4-nitrophenol + laurate - ? 363891 3.1.1.3 4-nitrophenyl myristate + H2O best 4-nitrophenyl ester substrate Psychrobacter sp. 4-nitrophenol + myristate - ? 364082 3.1.1.3 4-nitrophenyl palmitate + H2O - Psychrobacter sp. 4-nitrophenol + palmitate - ? 363890 3.1.1.3 4-nitrophenyl palmitate + H2O high activity Psychrobacter sp. 4-nitrophenol + palmitate - ? 363890 3.1.1.3 4-nitrophenyl stearate + H2O - Psychrobacter sp. 4-nitrophenol + stearate - ? 364135 3.1.1.3 additional information substrate specificity, LipA1 shows a preference for trimyristin and 4-nitrophenyl myristate, overview Psychrobacter sp. ? - ? 89 3.1.1.3 olive oil + H2O high activity Psychrobacter sp. ? - ? 364063 3.1.1.3 triacetin + H2O - Psychrobacter sp. diacetin + acetate - ? 364163 3.1.1.3 tributyrin + H2O - Psychrobacter sp. dibutyrin + butyrate - ? 363949 3.1.1.3 tricaprin + H2O - Psychrobacter sp. dicaprin + caprate - ? 364183 3.1.1.3 trimyristin + H2O best triacylglyceride substrate Psychrobacter sp. dimyristin + myristate - ? 364132 3.1.1.3 triolein + H2O - Psychrobacter sp. diolein + oleate - ? 363933 3.1.1.3 tripalmitin + H2O high activity Psychrobacter sp. dipalmitin + palmitate - ? 364080 3.1.1.3 tristearin + H2O - Psychrobacter sp. distearin + stearate - ? 364134 3.1.1.3 4-nitrophenyl acetate + H2O - Pyrococcus furiosus 4-nitrophenol + acetate - ? 12514 3.1.1.3 4-nitrophenyl butyrate + H2O - Pyrococcus furiosus 4-nitrophenol + butyrate - ? 115376 3.1.1.3 4-nitrophenyl palmitate + H2O - Pyrococcus furiosus 4-nitrophenol + palmitate - ? 363890 3.1.1.3 4-nitrophenyl caprylate + H2O preferred 4-nitrophenyl substrate of the recombinant enzyme Ralstonia sp. 4-nitrophenol + caprylate - ? 363889 3.1.1.3 4-nitrophenyl myristate + H2O preferred 4-nitrophenyl substrate of the wild-type enzyme Ralstonia sp. 4-nitrophenol + myristate - ? 364082 3.1.1.3 additional information substrate specificity of the recombinant and the wild-type enzyme, preference for triacylglycerides with short length fatty acids Ralstonia sp. ? - ? 89 3.1.1.3 triacylglycerol + H2O of several natural oils, e.g. cottonseed oil, corn oil, palm oil, wheatgerm oil, soybean oil, rapeseed oil, coconut oil, linseed oil, peanut oil, and olive oil in descending activity, overview Ralstonia sp. diacylglycerol + a carboxylate - ? 360693 3.1.1.3 tributyrin + H2O - Ralstonia sp. dibutyrin + butyrate - ? 363949 3.1.1.3 tricaprylin + H2O - Ralstonia sp. dicaprylin + caprylate - ? 364128 3.1.1.3 triolein + H2O olive oil Ralstonia sp. diolein + oleate - ? 363933 3.1.1.3 4-nitrophenyl caprylate + H2O preferred 4-nitrophenyl substrate of the recombinant enzyme Ralstonia sp. M1 4-nitrophenol + caprylate - ? 363889 3.1.1.3 additional information substrate specificity of the recombinant and the wild-type enzyme, preference for triacylglycerides with short length fatty acids Ralstonia sp. M1 ? - ? 89 3.1.1.3 triacylglycerol + H2O of several natural oils, e.g. cottonseed oil, corn oil, palm oil, wheatgerm oil, soybean oil, rapeseed oil, coconut oil, linseed oil, peanut oil, and olive oil in descending activity, overview Ralstonia sp. M1 diacylglycerol + a carboxylate - ? 360693 3.1.1.3 tributyrin + H2O - Ralstonia sp. M1 dibutyrin + butyrate - ? 363949 3.1.1.3 triolein + H2O olive oil Ralstonia sp. M1 diolein + oleate - ? 363933 3.1.1.3 1,2-dioleoylglycerol + H2O - Rattus norvegicus oleic acid + ? - ? 64393 3.1.1.3 ethyl laurate + H2O - Rattus norvegicus ethanol + lauric acid - ? 64418 3.1.1.3 ethyl myristate + H2O - Rattus norvegicus ethanol + myristic acid - ? 64419 3.1.1.3 hexadecyl butyrate + H2O - Rattus norvegicus hexadecanol + butyric acid - ? 64416 3.1.1.3 hexadecyl formate + H2O - Rattus norvegicus hexadecanol + formic acid - ? 64414 3.1.1.3 hexadecyl propionate + H2O - Rattus norvegicus hexadecanol + propionic acid - ? 64415 3.1.1.3 methyl myristate + H2O - Rattus norvegicus methanol + myristic acid - ? 64417 3.1.1.3 monooleoylglycerol + H2O - Rattus norvegicus ? - ? 64392 3.1.1.3 additional information the enzyme is involved in basal lipolysis of storage triacylglycerides, and hormonally as well as developmentally regulated, physiological functions, role in apoB-containing lipoprotein assembly in the endoplasmic reticulum, overview Rattus norvegicus ? - ? 89 3.1.1.3 phosphatidic acid + H2O - Rattus norvegicus ? - ? 64437 3.1.1.3 phosphatidylcholine + H2O - Rattus norvegicus ? - ? 64439 3.1.1.3 phosphatidylethanolamine + H2O - Rattus norvegicus ? - ? 64438 3.1.1.3 phosphatidylserine + H2O - Rattus norvegicus ? - ? 64440 3.1.1.3 retinyl palmitate + H2O - Rattus norvegicus retinol + palmitate - ? 115251 3.1.1.3 tetradecyl acetate + H2O - Rattus norvegicus tetradecanol + acetic acid - ? 64412 3.1.1.3 tetradecyl butyrate + H2O - Rattus norvegicus tetradecanol + butyric acid - ? 64413 3.1.1.3 triolein + H2O - Rattus norvegicus diolein + oleic acid - ? 364170 3.1.1.3 trioleoylglycerol + H2O - Rattus norvegicus oleic acid + ? - ? 64389 3.1.1.3 (4S)-4-tert-butyl-2-phenyl-1,3-oxazol-5(4H)-one + butanol - Rhizomucor miehei butyl (2S)-2-(benzoylamino)3,3-dimethylbutanoate - ? 375444 3.1.1.3 1,2-O-dilauryl-rac-glycero-3-glutaric acid resorufin ester + H2O - Rhizomucor miehei ? - ? 364152 3.1.1.3 4-nitrophenyl butanoate + H2O - Rhizomucor miehei 4-nitrophenol + butanoate - ? 363828 3.1.1.3 4-nitrophenyl butyrate + H2O low activity Rhizomucor miehei 4-nitrophenol + butyrate - ? 115376 3.1.1.3 4-nitrophenyl decanoate + H2O - Rhizomucor miehei 4-nitrophenol + decanoate - ? 360778 3.1.1.3 4-nitrophenyl hexanoate + H2O low activity Rhizomucor miehei 4-nitrophenol + hexanoate - ? 115379 3.1.1.3 4-nitrophenyl octanoate + H2O - Rhizomucor miehei 4-nitrophenol + octanoate - ? 115380 3.1.1.3 4-nitrophenyl octanoate + H2O low activity Rhizomucor miehei 4-nitrophenol + octanoate - ? 115380 3.1.1.3 4-nitrophenyl tetradecanoate + H2O best 4-nitrophenyl ester substrate for wild-type enzyme and mutant F94R Rhizomucor miehei 4-nitrophenol + tetradecanoate - ? 363830 3.1.1.3 eugenol + benzoic acid - Rhizomucor miehei eugenyl benzoate - ? 441385 3.1.1.3 glycerol + (R)-mandelic acid methyl ester + H2O about 300fold lower activity compared to phenylpropionic acid ethyl ester Rhizomucor miehei ? - ? 417047 3.1.1.3 glycerol + (S)-mandelic acid methyl ester + H2O about 300fold lower activity compared to phenylpropionic acid ethyl ester Rhizomucor miehei ? - ? 417048 3.1.1.3 glycerol + phenylacetic acid methyl ester + H2O about 5fold lower activity compared to phenylpropionic acid ethyl ester Rhizomucor miehei ? - ? 417053 3.1.1.3 glycerol + phenylmalonic acid dimethyl ester + H2O about 5fold lower activity compared to phenylpropionic acid ethyl ester Rhizomucor miehei glyceryl monomethylphenylmalonate + ethanol - ? 417054 3.1.1.3 glycerol + phenylpropionic acid ethyl ester + H2O the immobilized lipase exhibits the highest activity towards phenylpropionic acid ethyl ester. 90% of glycerol is the optimum amount to perform the transesterification reaction Rhizomucor miehei glyceryl phenylpropionic ester + ethanol the maximum product yield is achieved after 4 h (78%) ? 417055 3.1.1.3 high linoleic sunflower oil + H2O - Rhizomucor miehei ? - ? 401143 3.1.1.3 high oleic sunflower oil + H2O - Rhizomucor miehei ? - ? 401146 3.1.1.3 lauric acid + 1-propanol - Rhizomucor miehei propyl laurate + H2O - ? 401308 3.1.1.3 additional information enzyme does not possess protease activity and does not hydrolyze peptide bonds, no activity with N-benzoyl-L-tyrosine 4-nitroanilide Rhizomucor miehei ? - ? 89 3.1.1.3 additional information immobilized enzyme performs the transesterification reaction that replaces pamitic acid in palm oil with stearic acid Rhizomucor miehei ? - ? 89 3.1.1.3 additional information substrate chain length specificity of wild-type and mutant enzymes Rhizomucor miehei ? - ? 89 3.1.1.3 additional information synthesis of pentyl butanoate using two-step addition of acid substrate with immobilized lipase, overview, substrate polarity has an effect on the lipase-catalyzed synthesis of aroma esters in solvent-free systems, solvent-free synthesis enzyme inactivation by acid substrate Rhizomucor miehei ? - ? 89 3.1.1.3 octanol + decanoate - Rhizomucor miehei octyl decanoate + H2O - ? 441799 3.1.1.3 olive oil + H2O - Rhizomucor miehei ? - ? 364063 3.1.1.3 triacylglycerol + H2O - Rhizomucor miehei diacylglycerol + a carboxylate - ? 360693 3.1.1.3 tributyrin + H2O - Rhizomucor miehei dibutyrin + butyrate - ? 363949 3.1.1.3 tributyrin + H2O best triacylglyceride substrate for the wild-type enzyme Rhizomucor miehei dibutyrin + butyrate - ? 363949 3.1.1.3 tridecanin + H2O - Rhizomucor miehei didecanin + decanoate - ? 364130 3.1.1.3 trihexanin + H2O - Rhizomucor miehei dihexanin + hexanoate - ? 364129 3.1.1.3 trioctanin + H2O - Rhizomucor miehei dioctanin + octanoate - ? 364153 3.1.1.3 triolein + H2O - Rhizomucor miehei diolein + oleate - ? 363933 3.1.1.3 trioleoylglycerol + succinic acid - Rhizomucor miehei 1,2-dioleoyl-3-succinoylglycerol + 2-oleoyl-1,3-succinoylglycerol - ? 430743 3.1.1.3 tripalmitoylglycerol + succinic acid - Rhizomucor miehei 1,2-dipalmitoyl-3-succinoylglycerol + 2-palmitoyl-1,3-disuccinoylglycerol - ? 430745 3.1.1.3 (S)-glycidol + vinyl n-butyrate transesterification Rhizopus arrhizus (2R)-oxiran-2-yl-methyl butanoate + ethenol - ? 399227 3.1.1.3 1,2-didecanoyl-rac-glycerol + H2O - Rhizopus arrhizus ? - ? 64411 3.1.1.3 dicaprin + H2O the three dicaprin isomers spread at the air-water interface, ROL29 prefers the distal ester groups of diglyceride isomer 1,3-sn-dicaprin, ROL32 is more stereoselective than ROL29 for the sn-3 position of the 2,3-sn-enantiomer of dicaprin Rhizopus arrhizus caprin + caprate - ? 381799 3.1.1.3 high linoleic sunflower oil + H2O - Rhizopus arrhizus ? - ? 401143 3.1.1.3 high oleic sunflower oil + H2O - Rhizopus arrhizus ? - ? 401146 3.1.1.3 additional information enzyme does not possess protease activity and does not hydrolyze peptide bonds, no activity with N-benzoyl-L-tyrosine 4-nitroanilide Rhizopus arrhizus ? - ? 89 3.1.1.3 additional information substrate specificity, regioselectivity, and stereoselectivity of ROL29 and ROL32, the N-terminal peptide of the enzymes plays a role, overview, interaction of ROL29 and ROL32 with egg-yolk phosphocholine layer and penetration velocity Rhizopus arrhizus ? - ? 89 3.1.1.3 additional information ROLw is unable to hydrolyse triacylglycerols in the presence of high concentration of bile salts, it is a serine enzyme Rhizopus arrhizus ? - ? 89 3.1.1.3 oleic acid + n-butanol - Rhizopus arrhizus ? - ? 401562 3.1.1.3 olive oil + H2O - Rhizopus arrhizus ? - ? 364063 3.1.1.3 tributyrin + H2O - Rhizopus arrhizus dibutyrin + butyrate - ? 363949 3.1.1.3 triolein + H2O - Rhizopus arrhizus diolein + oleate - ? 363933 3.1.1.3 tripropionin + H2O - Rhizopus arrhizus dipropionin + propionate - ? 364079 3.1.1.3 additional information ROLw is unable to hydrolyse triacylglycerols in the presence of high concentration of bile salts, it is a serine enzyme Rhizopus arrhizus WPG ? - ? 89 3.1.1.3 olive oil + H2O - Rhizopus arrhizus WPG ? - ? 364063 3.1.1.3 tributyrin + H2O - Rhizopus arrhizus WPG dibutyrin + butyrate - ? 363949 3.1.1.3 trioctanoin + H2O - Rhizopus homothallicus dioctanoin + octanoate - ? 393712 3.1.1.3 additional information enzyme does not possess protease activity and does not hydrolyze peptide bonds, no activity with N-benzoyl-L-tyrosine 4-nitroanilide Rhizopus japonicus ? - ? 89 3.1.1.3 tributyrin + H2O - Rhizopus japonicus dibutyrin + butyrate - ? 363949 3.1.1.3 tricaprin + H2O - Rhizopus japonicus ? - ? 64399 3.1.1.3 tricaprylin + H2O - Rhizopus japonicus ? - ? 64400 3.1.1.3 tripalmitin + H2O - Rhizopus japonicus palmitic acid + ? - ? 64402 3.1.1.3 tristearin + H2O - Rhizopus japonicus stearic acid + ? - ? 64403 3.1.1.3 tricaprin + H2O - Rhizopus japonicus NR 400 ? - ? 64399 3.1.1.3 tricaprylin + H2O - Rhizopus japonicus NR 400 ? - ? 64400 3.1.1.3 tripalmitin + H2O - Rhizopus japonicus NR 400 palmitic acid + ? - ? 64402 3.1.1.3 tristearin + H2O - Rhizopus japonicus NR 400 stearic acid + ? - ? 64403 3.1.1.3 4-nitrophenyl acetate + H2O - Rhizopus microsporus var. chinensis 4-nitrophenol + acetate - ? 12514 3.1.1.3 4-nitrophenyl butyrate + H2O - Rhizopus microsporus var. chinensis 4-nitrophenol + butyrate - ? 115376 3.1.1.3 4-nitrophenyl caproate + H2O - Rhizopus microsporus var. chinensis 4-nitrophenol + caproate - ? 363887 3.1.1.3 4-nitrophenyl caprylate + H2O - Rhizopus microsporus var. chinensis 4-nitrophenol + caprylate - ? 363889 3.1.1.3 4-nitrophenyl laurate + H2O - Rhizopus microsporus var. chinensis 4-nitrophenol + laurate - ? 363891 3.1.1.3 4-nitrophenyl palmitate + H2O preferred substrate Rhizopus microsporus var. chinensis 4-nitrophenol + palmitate - ? 363890 3.1.1.3 4-nitrophenyl propionate + H2O - Rhizopus microsporus var. chinensis 4-nitrophenol + propionate - ? 115374 3.1.1.3 4-nitrophenyl stearate + H2O - Rhizopus microsporus var. chinensis 4-nitrophenol + stearate - ? 364135 3.1.1.3 high linoleic sunflower oil + H2O - Rhizopus niveus ? - ? 401143 3.1.1.3 high oleic sunflower oil + H2O - Rhizopus niveus ? - ? 401146 3.1.1.3 4-nitrophenyl butyrate + H2O - Rhodotorula glutinis 4-nitrophenol + butyrate - ? 115376 3.1.1.3 4-nitrophenyl laurate + H2O - Rhodotorula glutinis 4-nitrophenol + laurate - ? 363891 3.1.1.3 tributyrin + H2O - Rhodotorula glutinis dibutyrin + butyrate - ? 363949 3.1.1.3 additional information specific for the primary position of the glycerides. Triglycerides with C18 are hydrolyzed in this order: C18:3, C18:2, C18:1, C18:0 Rhodotorula mucilaginosa ? - ? 89 3.1.1.3 4-nitrophenyl butyrate + H2O high activity Ricinus communis 4-nitrophenol + butyrate - ? 115376 3.1.1.3 4-nitrophenyl laurate + H2O - Ricinus communis 4-nitrophenol + laurate - ? 363891 3.1.1.3 4-nitrophenyl palmitate + H2O low activity Ricinus communis 4-nitrophenol + palmitate - ? 363890 3.1.1.3 additional information the enzyme is involved in regulation of lipolysis Ricinus communis ? - ? 89 3.1.1.3 additional information substrate specificity, no activity with oleoyl-CoA and 1,2-dioleoyl-3-phosphatidylcholine Ricinus communis ? - ? 89 3.1.1.3 tricaprin + H2O - Ricinus communis dicaprin + caprate - ? 364183 3.1.1.3 trilaurin + H2O - Ricinus communis dilaurin + laurate - ? 364081 3.1.1.3 trilinolein + H2O - Ricinus communis dilinolein + linoleate - ? 364139 3.1.1.3 triolein + H2O - Ricinus communis diolein + oleate - ? 363933 3.1.1.3 tripalmitin + H2O - Ricinus communis dipalmitin + palmitate - ? 364080 3.1.1.3 triricinolein + H2O - Ricinus communis diricinolein + ricinolate - ? 382905 3.1.1.3 4-nitrophenyl butanoate + H2O - Saccharolobus solfataricus 4-nitrophenol + butanoate - ? 363828 3.1.1.3 4-nitrophenyl caprate + H2O - Saccharolobus solfataricus 4-nitrophenol + caprate - ? 364083 3.1.1.3 4-nitrophenyl palmitate + H2O - Saccharolobus solfataricus 4-nitrophenol + palmitate best substrate ? 363890 3.1.1.3 additional information enzyme shows broad substrate specificity, exhibiting carboxylesterase activity towards short-chain acyl esters and lipase activity toward long-chain acyl esters including triacylglycerols regardless of saturated and unsaturated fatty acids Saccharolobus solfataricus ? - ? 89 3.1.1.3 triolein + 3 H2O - Saccharolobus solfataricus glycerol + 3 oleate enzyme can hydrolyze all positions of the ester bonds in triolein ? 434444 3.1.1.3 4-nitrophenyl butanoate + H2O - Saccharolobus solfataricus DSM 1616 4-nitrophenol + butanoate - ? 363828 3.1.1.3 4-nitrophenyl caprate + H2O - Saccharolobus solfataricus DSM 1616 4-nitrophenol + caprate - ? 364083 3.1.1.3 4-nitrophenyl palmitate + H2O - Saccharolobus solfataricus DSM 1616 4-nitrophenol + palmitate best substrate ? 363890 3.1.1.3 additional information enzyme shows broad substrate specificity, exhibiting carboxylesterase activity towards short-chain acyl esters and lipase activity toward long-chain acyl esters including triacylglycerols regardless of saturated and unsaturated fatty acids Saccharolobus solfataricus DSM 1616 ? - ? 89 3.1.1.3 triolein + 3 H2O - Saccharolobus solfataricus DSM 1616 glycerol + 3 oleate enzyme can hydrolyze all positions of the ester bonds in triolein ? 434444 3.1.1.3 1,2,3-trihexaicosanoylglycerol + H2O - Saccharomyces cerevisiae 1,2-dihexaicosanoylglycerol + hexaicosanoate - ? 382899 3.1.1.3 4-nitrophenyl acetate + H2O - Saccharomyces cerevisiae 4-nitrophenol + acetate - ? 12514 3.1.1.3 4-nitrophenyl butyrate + H2O - Saccharomyces cerevisiae 4-nitrophenol + butyrate - ? 115376 3.1.1.3 additional information substrate specificity of isozymes, overview Saccharomyces cerevisiae ? - ? 89 3.1.1.3 additional information the enzyme is required for mobilization of triacylglycerides stored in lipid particles, overview Saccharomyces cerevisiae ? - ? 89 3.1.1.3 triacylglycerol + H2O - Saccharomyces cerevisiae diacylglycerol + a carboxylate - ? 360693 3.1.1.3 trilinolein + H2O - Saccharomyces cerevisiae dilinolein + linoleate - ? 364139 3.1.1.3 trimyristin + H2O - Saccharomyces cerevisiae dimyristin + myristate - ? 364132 3.1.1.3 triolein + H2O - Saccharomyces cerevisiae ? - ? 64454 3.1.1.3 triolein + H2O - Saccharomyces cerevisiae diolein + oleate - ? 363933 3.1.1.3 tripalmitin + H2O - Saccharomyces cerevisiae dipalmitin + palmitate - ? 364080 3.1.1.3 tristearin + H2O - Saccharomyces cerevisiae distearin + stearate - ? 364134 3.1.1.3 additional information substrate specificity of isozymes, overview Saccharomyces cerevisiae BY4741 ? - ? 89 3.1.1.3 additional information the enzyme is required for mobilization of triacylglycerides stored in lipid particles, overview Saccharomyces cerevisiae BY4741 ? - ? 89 3.1.1.3 triacylglycerol + H2O - Saccharomyces cerevisiae BY4741 diacylglycerol + a carboxylate - ? 360693 3.1.1.3 trimyristin + H2O - Saccharomyces cerevisiae BY4741 dimyristin + myristate - ? 364132 3.1.1.3 triolein + H2O - Saccharomyces cerevisiae BY4741 diolein + oleate - ? 363933 3.1.1.3 tripalmitin + H2O - Saccharomyces cerevisiae BY4741 dipalmitin + palmitate - ? 364080 3.1.1.3 4-nitrophenyl acetate + H2O - Saccharomyces cerevisiae TM 4-nitrophenol + acetate - ? 12514 3.1.1.3 4-nitrophenyl butyrate + H2O - Saccharomyces cerevisiae TM 4-nitrophenol + butyrate - ? 115376 3.1.1.3 triacylglycerol + H2O - Saccharomyces cerevisiae TM diacylglycerol + a carboxylate - ? 360693 3.1.1.3 additional information the enzyme is more active on short-chain triacylglycerols than on long-chain ones Sardinella aurita ? - ? 89 3.1.1.3 1,2-sn-dicaprin + H2O regio- and stereoselective reaction Scorpio maurus ? - ? 390507 3.1.1.3 2,3-sn-dicaprin + H2O regio- and stereoselective reaction Scorpio maurus ? - ? 390589 3.1.1.3 additional information substrate specificity, lipase penetration into the egg-phosphocholine monolayer, overview Scorpio maurus ? - ? 89 3.1.1.3 olive oil + H2O - Scorpio maurus ? - ? 364063 3.1.1.3 tributyrin + H2O - Scorpio maurus dibutyrin + butyrate - ? 363949 3.1.1.3 triolein + H2O olive oil Scorpio maurus diolein + oleate - ? 363933 3.1.1.3 tripropionin + H2O - Scorpio maurus dipropionin + propionate - ? 364079 3.1.1.3 (+/-)-trans-3-(4'-methoxy-phenyl)-glycidyl methyl ester + H2O 50% conversion yield after 4 h in 100 mM potassium phosphate buffer, pH 7.5, at 30 °C Serratia marcescens (2R,3S)-3-(4-hydroxyphenyl)oxirane-2-carboxylic acid + methyl (2S,3R)-3-(4-hydroxyphenyl)oxirane-2-carboxylate + methanol - ? 415807 3.1.1.3 (R,S)-flurbiprofen ethyl ester + H2O enantioselective hydrolysis, kinetic resolution, overview Serratia marcescens (S)-flurbiprofen + ethanol - ? 390462 3.1.1.3 (R,S)-flurbiprofen ethyl ester + H2O high enantiomeric excess and conversion yield of 98% and 48%, respectively Serratia marcescens (S)-flurbiprofen + ethanol - ? 390462 3.1.1.3 3-(4-methoxyphenyl)glycidic acid methyl ester + H2O - Serratia marcescens (2R,3S)-3-(4-methoxyphenyl)glycidic acid methyl ester + (2S,3R)-3-(4-methoxyphenyl)glycidic acid + methanol - ? 375794 3.1.1.3 4-hydroxy-3-methyl-2-(2-propenyl)-2-cyclopenten-1-one acetate + H2O 50% conversion Serratia marcescens ? - ? 399654 3.1.1.3 4-nitrophenyl acetate + H2O - Serratia marcescens 4-nitrophenol + acetate - ? 12514 3.1.1.3 4-nitrophenyl acetate + H2O worst substrate, less than 5% activity compared to 4-nitrophenyl laurate Serratia marcescens 4-nitrophenol + acetate - ? 12514 3.1.1.3 4-nitrophenyl butyrate + H2O - Serratia marcescens 4-nitrophenol + butyrate - ? 115376 3.1.1.3 4-nitrophenyl butyrate + H2O about 10% activity compared to 4-nitrophenyl laurate Serratia marcescens 4-nitrophenol + butyrate - ? 115376 3.1.1.3 4-nitrophenyl caprate + H2O high activity Serratia marcescens 4-nitrophenol + caprate - ? 364083 3.1.1.3 4-nitrophenyl caproate + H2O high activity Serratia marcescens 4-nitrophenol + caproate - ? 363887 3.1.1.3 4-nitrophenyl caprylate + H2O best 4-nitrophenyl ester substrate Serratia marcescens 4-nitrophenol + caprylate - ? 363889 3.1.1.3 4-nitrophenyl decanoate + H2O - Serratia marcescens 4-nitrophenol + decanoate - ? 360778 3.1.1.3 4-nitrophenyl decanoate + H2O about 30% activity compared to 4-nitrophenyl laurate Serratia marcescens 4-nitrophenol + decanoate - ? 360778 3.1.1.3 4-nitrophenyl hexanoate + H2O - Serratia marcescens 4-nitrophenol + hexanoate - ? 115379 3.1.1.3 4-nitrophenyl laurate + H2O - Serratia marcescens 4-nitrophenol + laurate - ? 363891 3.1.1.3 4-nitrophenyl laurate + H2O maximum activity on 4-nitrophenyl laurate Serratia marcescens 4-nitrophenol + laurate - ? 363891 3.1.1.3 4-nitrophenyl myristate + H2O - Serratia marcescens 4-nitrophenol + myristate - ? 364082 3.1.1.3 4-nitrophenyl myristate + H2O the enzyme shows maximum activity on 4-nitrophenyl myristate Serratia marcescens 4-nitrophenol + myristate - ? 364082 3.1.1.3 4-nitrophenyl myristate + H2O about 90% activity compared to 4-nitrophenyl laurate Serratia marcescens 4-nitrophenol + myristate - ? 364082 3.1.1.3 4-nitrophenyl palmitate + H2O - Serratia marcescens 4-nitrophenol + palmitate - ? 363890 3.1.1.3 4-nitrophenyl palmitate + H2O low activity Serratia marcescens 4-nitrophenol + palmitate - ? 363890 3.1.1.3 4-nitrophenyl palmitate + H2O about 30% activity compared to 4-nitrophenyl laurate Serratia marcescens 4-nitrophenol + palmitate - ? 363890 3.1.1.3 4-nitrophenyl propionate + H2O - Serratia marcescens 4-nitrophenol + propionate - ? 115374 3.1.1.3 4-nitrophenyl propionate + H2O low activity Serratia marcescens 4-nitrophenol + propionate - ? 115374 3.1.1.3 4-nitrophenyl propionate + H2O about 5% activity compared to 4-nitrophenyl laurate Serratia marcescens 4-nitrophenol + propionate - ? 115374 3.1.1.3 4-phenyl-[1,3]-dioxolan-2-one + H2O 9.6% conversion Serratia marcescens ? - ? 399704 3.1.1.3 glycidyl butyrate + H2O 49% conversion Serratia marcescens ? - ? 401075 3.1.1.3 methyl acetate + H2O 25% conversion Serratia marcescens ? - ? 401378 3.1.1.3 methyl mandelate + H2O 33% conversion Serratia marcescens ? - ? 401380 3.1.1.3 additional information substrate specificity, no or poor activity with tributyrin, triacetin, tripropionin, tristearin, 4-nitrophenyl stearate, and 4-nitrophenyl acetate, the lipase shows preferential substrate specificity toward the medium-chain-length fatty acids, overview Serratia marcescens ? - ? 89 3.1.1.3 additional information does not hydolyze propylene carbonate Serratia marcescens ? - ? 89 3.1.1.3 naproxen methyl ester + H2O 41% conversion Serratia marcescens ? - ? 401521 3.1.1.3 trans-3-(4'-methoxyphenyl) glycidic acid methyl ester + H2O 60% conversion Serratia marcescens ? - ? 402038 3.1.1.3 tricaprin + H2O best triacylglyceride substrate Serratia marcescens dicaprin + caprate - ? 364183 3.1.1.3 tricaproin + H2O - Serratia marcescens dicaproin + caproate - ? 364147 3.1.1.3 tricaprylin + H2O - Serratia marcescens dicaprylin + caprylate - ? 364128 3.1.1.3 trilaurin + H2O high activity Serratia marcescens dilaurin + laurate - ? 364081 3.1.1.3 trimyristin + H2O - Serratia marcescens dimyristin + myristate - ? 364132 3.1.1.3 tripalmitin + H2O low activity Serratia marcescens dipalmitin + palmitate - ? 364080 3.1.1.3 4-nitrophenyl acetate + H2O worst substrate, less than 5% activity compared to 4-nitrophenyl laurate Serratia marcescens ECU1010 4-nitrophenol + acetate - ? 12514 3.1.1.3 4-nitrophenyl acetate + H2O - Serratia marcescens ECU1010 4-nitrophenol + acetate - ? 12514 3.1.1.3 4-nitrophenyl butyrate + H2O about 10% activity compared to 4-nitrophenyl laurate Serratia marcescens ECU1010 4-nitrophenol + butyrate - ? 115376 3.1.1.3 4-nitrophenyl butyrate + H2O - Serratia marcescens ECU1010 4-nitrophenol + butyrate - ? 115376 3.1.1.3 4-nitrophenyl decanoate + H2O about 30% activity compared to 4-nitrophenyl laurate Serratia marcescens ECU1010 4-nitrophenol + decanoate - ? 360778 3.1.1.3 4-nitrophenyl hexanoate + H2O - Serratia marcescens ECU1010 4-nitrophenol + hexanoate - ? 115379 3.1.1.3 4-nitrophenyl palmitate + H2O about 30% activity compared to 4-nitrophenyl laurate Serratia marcescens ECU1010 4-nitrophenol + palmitate - ? 363890 3.1.1.3 4-nitrophenyl propionate + H2O about 5% activity compared to 4-nitrophenyl laurate Serratia marcescens ECU1010 4-nitrophenol + propionate - ? 115374 3.1.1.3 4-nitrophenyl propionate + H2O - Serratia marcescens ECU1010 4-nitrophenol + propionate - ? 115374 3.1.1.3 additional information does not hydolyze propylene carbonate Serratia marcescens ECU1010 ? - ? 89 3.1.1.3 (R,S)-flurbiprofen ethyl ester + H2O enantioselective hydrolysis, kinetic resolution, overview Serratia marcescens ES-2 (S)-flurbiprofen + ethanol - ? 390462 3.1.1.3 4-nitrophenyl butyrate + H2O - Serratia marcescens ES-2 4-nitrophenol + butyrate - ? 115376 3.1.1.3 4-nitrophenyl caproate + H2O high activity Serratia marcescens ES-2 4-nitrophenol + caproate - ? 363887 3.1.1.3 4-nitrophenyl caprylate + H2O best 4-nitrophenyl ester substrate Serratia marcescens ES-2 4-nitrophenol + caprylate - ? 363889 3.1.1.3 4-nitrophenyl propionate + H2O low activity Serratia marcescens ES-2 4-nitrophenol + propionate - ? 115374 3.1.1.3 additional information substrate specificity, no or poor activity with tributyrin, triacetin, tripropionin, tristearin, 4-nitrophenyl stearate, and 4-nitrophenyl acetate, the lipase shows preferential substrate specificity toward the medium-chain-length fatty acids, overview Serratia marcescens ES-2 ? - ? 89 3.1.1.3 3-(4-methoxyphenyl)glycidic acid methyl ester + H2O - Serratia marcescens Sr41 8000 (2R,3S)-3-(4-methoxyphenyl)glycidic acid methyl ester + (2S,3R)-3-(4-methoxyphenyl)glycidic acid + methanol - ? 375794 3.1.1.3 additional information the enzyme probably is involved in fat mobilization during seed germination Solanum lycopersicum ? - ? 89 3.1.1.3 additional information substrate specificity, the enzyme catalyzes hydrolysis of triacylglycerides with long fatty acid chains, low activity with tripalmitin and tristearin, no activity with tributyrin and triacetate, phosphatidylcholine and monogalactosyldiacylglycerol are poor substrates Solanum lycopersicum ? - ? 89 3.1.1.3 tricaprylin + H2O - Solanum lycopersicum dicaprylin + caprylate - ? 364128 3.1.1.3 triolein + H2O - Solanum lycopersicum diolein + oleate - ? 363933 3.1.1.3 tripalmitin + H2O low activity Solanum lycopersicum dipalmitin + palmitate - ? 364080 3.1.1.3 tristearin + H2O low activity Solanum lycopersicum distearin + stearate - ? 364134 3.1.1.3 trioleoylglycerol + H2O - Staphylococcus aureus oleic acid + ? - ? 64389 3.1.1.3 trioleoylglycerol + H2O - Staphylococcus aureus FN 37 oleic acid + ? - ? 64389 3.1.1.3 tributyrin + H2O - Staphylococcus epidermidis dibutyrin + butyrate - ? 363949 3.1.1.3 additional information Ssp is not responsible for binding to collagen type I by the cell Staphylococcus saprophyticus ? - ? 89 3.1.1.3 tributyrin + H2O - Staphylococcus saprophyticus dibutyrin + butyrate - ? 363949 3.1.1.3 additional information enzyme hydrolyzes triacylglycerols without chain length specificity Staphylococcus simulans ? - ? 89 3.1.1.3 tributyrin + H2O - Staphylococcus simulans dibutyrin + butyrate - ? 363949 3.1.1.3 triolein + H2O - Staphylococcus simulans diolein + oleate - ? 363933 3.1.1.3 tripropionin + H2O - Staphylococcus simulans dipropionin + propionate - ? 364079 3.1.1.3 1,2-dioctanoyl-sn-glycero-3-phosphocholine + H2O - Staphylococcus warneri ? - ? 73304 3.1.1.3 1,2-dioctanoyl-sn-glycero-3-phosphoglycol + H2O - Staphylococcus warneri ? - ? 364127 3.1.1.3 2-hexandecanoylthio-ethane-1-phosphocholine + H2O - Staphylococcus warneri ? - ? 364126 3.1.1.3 4-nitrophenyl butyrate + H2O - Staphylococcus warneri 4-nitrophenol + butyrate - ? 115376 3.1.1.3 additional information substrate specificity, the enzyme strongly prefers short-chain substrates, the enzyme SWL2 also shows phospholipase activity Staphylococcus warneri ? - ? 89 3.1.1.3 triolein + H2O low activity Staphylococcus warneri diolein + oleate - ? 363933 3.1.1.3 1,2-dioctanoyl-sn-glycero-3-phosphocholine + H2O - Staphylococcus warneri 863 ? - ? 73304 3.1.1.3 2-hexandecanoylthio-ethane-1-phosphocholine + H2O - Staphylococcus warneri 863 ? - ? 364126 3.1.1.3 4-nitrophenyl butyrate + H2O - Staphylococcus warneri 863 4-nitrophenol + butyrate - ? 115376 3.1.1.3 additional information substrate specificity, the enzyme strongly prefers short-chain substrates, the enzyme SWL2 also shows phospholipase activity Staphylococcus warneri 863 ? - ? 89 3.1.1.3 triolein + H2O low activity Staphylococcus warneri 863 diolein + oleate - ? 363933 3.1.1.3 4-nitrophenyl butyrate + H2O - Staphylococcus xylosus 4-nitrophenol + butyrate - ? 115376 3.1.1.3 additional information residue Asp290 is important for the chain length specificity and catalytic efficiency of the enzyme Staphylococcus xylosus ? - ? 89 3.1.1.3 additional information the enyzyme also uses olive oil as substrate Staphylococcus xylosus ? - ? 89 3.1.1.3 olive oil + H2O - Staphylococcus xylosus ? - ? 364063 3.1.1.3 tributyrin + H2O - Staphylococcus xylosus dibutyrin + butyrate - ? 363949 3.1.1.3 trioctanoate + H2O - Staphylococcus xylosus dioctanoate + octanoate - ? 393711 3.1.1.3 trioctanoin + H2O - Staphylococcus xylosus dioctanoin + octanoate - ? 393712 3.1.1.3 4-nitrophenyl caprate + H2O - Streptomyces coelicolor 4-nitrophenol + caprate - ? 364083 3.1.1.3 4-nitrophenyl laurate + H2O - Streptomyces coelicolor 4-nitrophenol + laurate - ? 363891 3.1.1.3 4-nitrophenyl palmitate + H2O - Streptomyces coelicolor 4-nitrophenol + palmitate - ? 363890 3.1.1.3 4-nitrophenyl stearate + H2O - Streptomyces coelicolor 4-nitrophenol + stearate - ? 364135 3.1.1.3 additional information isozyme SCO1725 shows no activity on tricaprin, tricaproin, and olive oil, 4-nitrophenyl stearate is hardly hydrolysed, 4-nitrophenyl stearate is no substrate for isozyme SCO1725 Streptomyces coelicolor ? - ? 89 3.1.1.3 tributyrin + H2O partially hydrolysed by isozyme SCO1725 Streptomyces coelicolor dibutyrin + butyrate - ? 363949 3.1.1.3 tricaprin + H2O - Streptomyces coelicolor dicaprin + decaprate - ? 364146 3.1.1.3 tricaprylin + H2O - Streptomyces coelicolor dicaprylin + caprylate - ? 364128 3.1.1.3 Tween 80 + H2O - Streptomyces coelicolor ? - ? 364144 3.1.1.3 4-nitrophenyl caprate + H2O - Streptomyces coelicolor A3(2) 4-nitrophenol + caprate - ? 364083 3.1.1.3 4-nitrophenyl laurate + H2O - Streptomyces coelicolor A3(2) 4-nitrophenol + laurate - ? 363891 3.1.1.3 4-nitrophenyl palmitate + H2O - Streptomyces coelicolor A3(2) 4-nitrophenol + palmitate - ? 363890 3.1.1.3 4-nitrophenyl stearate + H2O - Streptomyces coelicolor A3(2) 4-nitrophenol + stearate - ? 364135 3.1.1.3 additional information isozyme SCO1725 shows no activity on tricaprin, tricaproin, and olive oil, 4-nitrophenyl stearate is hardly hydrolysed, 4-nitrophenyl stearate is no substrate for isozyme SCO1725 Streptomyces coelicolor A3(2) ? - ? 89 3.1.1.3 tributyrin + H2O partially hydrolysed by isozyme SCO1725 Streptomyces coelicolor A3(2) dibutyrin + butyrate - ? 363949 3.1.1.3 tricaprylin + H2O - Streptomyces coelicolor A3(2) dicaprylin + caprylate - ? 364128 3.1.1.3 2,3-dimercapto-1-propanol tributyrate + H2O regiospecificity study Streptomyces rimosus ? - ? 364145 3.1.1.3 additional information enzyme also performs transesterification reactions, regio- and enantioselctivity, overview, substrate specificity: enzyme hydrolyzes most efficiently medium chain length fatty acid glycerol esters, C8-C12, and shows a preference for esters of C16 and C18 unsaturated fatty acids over C16 and C18 saturated fatty acid esters, as well as for triacylglycerol substrates with cis double bond, e.g. triolein, versus trans double bonds, e.g. trielaidin, enzyme also utilizes different natural plant oils as substrates, overview Streptomyces rimosus ? - ? 89 3.1.1.3 tributyrin + H2O - Streptomyces rimosus dibutyrin + butyrate - ? 363949 3.1.1.3 tricaprin + H2O best substrate Streptomyces rimosus dicaprin + capriate - ? 364136 3.1.1.3 tricaprylin + H2O best substrate Streptomyces rimosus dicaprylin + caprylate - ? 364128 3.1.1.3 trielaidin + H2O trans C18:1 Streptomyces rimosus dielaidin + elaidiate - ? 364138 3.1.1.3 trilinolein + H2O - Streptomyces rimosus dilinolein + linoleate - ? 364139 3.1.1.3 trilinolenin + H2O - Streptomyces rimosus dilinolenin + linolenate - ? 364140 3.1.1.3 triolein + H2O cis C18:1 Streptomyces rimosus diolein + oleate main product is oleic acid, further products are 1,3-dioleylglycerol, 1,2-dioleylglycerol, 2,3-dioleylglycerol, and minor product is 1-monooleylglycerol ? 363933 3.1.1.3 tripalmitin + H2O - Streptomyces rimosus dipalmitin + palmitate - ? 364080 3.1.1.3 tripalmitolein + H2O - Streptomyces rimosus dipalmitolein + palmitate - ? 364137 3.1.1.3 tristearin + H2O - Streptomyces rimosus distearin + stearate - ? 364134 3.1.1.3 Tween 20 + H2O i.e. poly(oxyethylene) sorbitan monolaurate Streptomyces rimosus ? - ? 364141 3.1.1.3 Tween 40 + H2O i.e. poly(oxyethylene) sorbitan monopalmitate Streptomyces rimosus ? - ? 364142 3.1.1.3 Tween 60 + H2O i.e. poly(oxyethylene) sorbitan monostearate Streptomyces rimosus ? - ? 364143 3.1.1.3 Tween 80 + H2O i.e. poly(oxyethylene) sorbitan monooleate Streptomyces rimosus ? - ? 364144 3.1.1.3 4-nitrophenyl palmitate + H2O preferred substrate Streptomyces sp. 4-nitrophenol + palmitate - ? 363890 3.1.1.3 4-nitrophenyl palmitate + H2O preferred substrate Streptomyces sp. CS326 4-nitrophenol + palmitate - ? 363890 3.1.1.3 liprocil + H2O - Struthio camelus ? - ? 382218 3.1.1.3 additional information the pancreatic enzyme shows a wide substrate spectrum but prefers triacylglyceride substrates with short fatty acid chains Struthio camelus ? - ? 89 3.1.1.3 tributyrin + H2O preferred substrate Struthio camelus dibutyrin + butyrate - ? 363949 3.1.1.3 tripropionin + H2O - Struthio camelus dipropionin + propanoate - ? 382901 3.1.1.3 1,2 di-O-lauryl-rac-glycero-3-(glutaric acid 6-methyl) resorufin ester + H2O - Sus scrofa ? - ? 429570 3.1.1.3 1,2-didecanoyl-rac-glycerol + H2O - Sus scrofa ? - ? 64411 3.1.1.3 1-caprin + H2O - Sus scrofa glycerol + caprate - ? 380840 3.1.1.3 1-olein + H2O - Sus scrofa glycerol + oleate - ? 380856 3.1.1.3 4-nitrophenyl palmitate + H2O - Sus scrofa 4-nitrophenol + palmitate - ? 363890 3.1.1.3 5-bromo-4-chloro-3-indoxyl palmitate + H2O - Sus scrofa ? - ? 399719 3.1.1.3 ethyl myristate + H2O - Sus scrofa ethanol + myristic acid - ? 64419 3.1.1.3 ethyl palmitate + H2O - Sus scrofa ethanol + palmitic acid - ? 64420 3.1.1.3 hexadecyl butyrate + H2O - Sus scrofa hexadecanol + butyric acid - ? 64416 3.1.1.3 hexadecyl formate + H2O - Sus scrofa hexadecanol + formic acid - ? 64414 3.1.1.3 hexadecyl propionate + H2O - Sus scrofa hexadecanol + propionic acid - ? 64415 3.1.1.3 high linoleic sunflower oil + H2O - Sus scrofa ? - ? 401143 3.1.1.3 high oleic sunflower oil + H2O - Sus scrofa ? - ? 401146 3.1.1.3 L-alpha-phosphatidylcholine + H2O - Sus scrofa ? - ? 401243 3.1.1.3 methyl myristate + H2O - Sus scrofa methanol + myristic acid - ? 64417 3.1.1.3 methylbutyrate + H2O - Sus scrofa methanol + butyric acid - ? 64430 3.1.1.3 monoacetylglycerol + H2O - Sus scrofa acetic acid + glycerol - ? 64410 3.1.1.3 additional information - Sus scrofa ? - ? 89 3.1.1.3 additional information methyl esters are cleaved by lipase 5 times faster than ethyl esters. The relative hydrolysis rates are rather low for esters of C3 and C4 alcohols, they increase abruptly for esters of n-hexyl alcohol, which are cleaved faster than methyl esters Sus scrofa ? - ? 89 3.1.1.3 additional information enzyme does not possess protease activity and does not hydrolyze peptide bonds, no activity with N-benzoyl-L-tyrosine 4-nitroanilide Sus scrofa ? - ? 89 3.1.1.3 additional information the enzyme is involved in basal lipolysis of storage triacylglycerides, and hormonally as well as developmentally regulated, physiological functions, role in apoB-containing lipoprotein assembly in the endoplasmic reticulum, overview Sus scrofa ? - ? 89 3.1.1.3 additional information pancreatic lipase-catalyzed esterification of n-butanoic acid in solvent-free system and in isooctane carried out with three alcohols: n-pentanol, n-heptanol and geraniol, overview, substrate polarity has an effect on the lipase-catalyzed synthesis of aroma esters in solvent-free systems, solvent-free synthesis enzyme inactivation by acid substrate Sus scrofa ? - ? 89 3.1.1.3 n-butyl palmitate + H2O - Sus scrofa n-butanol + palmitic acid - ? 64422 3.1.1.3 n-hexyl laurate + H2O - Sus scrofa n-hexanol + lauric acid - ? 64423 3.1.1.3 n-propyl myristate + H2O - Sus scrofa n-propanol + myristic acid - ? 64421 3.1.1.3 olive oil + H2O - Sus scrofa ? - ? 364063 3.1.1.3 p-nitrophenyl acetate + H2O - Sus scrofa p-nitrophenol + acetic acid - ? 64441 3.1.1.3 tetradecyl butyrate + H2O - Sus scrofa tetradecanol + butyric acid - ? 64413 3.1.1.3 triacetylglycerol + H2O - Sus scrofa acetic acid + ? - ? 64409 3.1.1.3 triacylglycerol + H2O - Sus scrofa diacylglycerol + a carboxylate - ? 360693 3.1.1.3 tributyrin + H2O - Sus scrofa butyric acid + ? - ? 64394 3.1.1.3 tributyrin + H2O - Sus scrofa ? - ? 64455 3.1.1.3 tributyrin + H2O - Sus scrofa dibutyrin + butyrate - ? 363949 3.1.1.3 tricaprin + H2O - Sus scrofa dicaprin + caprate - ? 364183 3.1.1.3 tricaprylin + H2O - Sus scrofa dicaprylin + caprylate - ? 364128 3.1.1.3 trihexanoin + H2O - Sus scrofa hexanoic acid + ? - ? 64397 3.1.1.3 trioctanoyl glycerol + H2O - Sus scrofa ? - ? 430742 3.1.1.3 triolein + H2O - Sus scrofa diolein + oleate - ? 363933 3.1.1.3 triolein + H2O substrate is an olive oil/gum arabic emulsion containing radiolabeled triolein in presence of bile acids and lecithin at rate-limiting concentrations, rapid exchange of the enzyme between two different triacylglycerol droplets measured by a non-exchanging enzyme inhibitor in one of the droplets Sus scrofa diolein + oleate - ? 363933 3.1.1.3 tripropionin + H2O - Sus scrofa propionic acid + ? - ? 64395 3.1.1.3 vitamin A acetate + H2O - Sus scrofa ? - ? 64442 3.1.1.3 olive oil + H2O - Testudo graeca nabeulensis ? - ? 364063 3.1.1.3 tributyrin + H2O - Testudo graeca nabeulensis dibutyrin + butyrate - ? 363949 3.1.1.3 p-nitrophenyl butyrate + H2O - Thermobifida fusca p-nitrophenol + butyrate - ? 382470 3.1.1.3 triolein + H2O - Thermobifida fusca 1,2-diolein + oleate - ? 393714 3.1.1.3 glyceryl tributanoate + H2O - Thermomyces dupontii glycerol + butanoate - ? 441423 3.1.1.3 glyceryl trioctanoate + H2O - Thermomyces dupontii glycerol + octanoate - ? 401073 3.1.1.3 additional information Talaromyces thermophilus lipase (TTL) also hydrolyzes monogalactosyl diacylglycerol (MGDG) and digalactosyl diacylglycerol (DGDG) substrates (EC 3.1.1.26) presented in various forms to the enzyme. The enzyme has galactolipase activity on galactolipid micelles, monomolecular films, and UV-absorbing surface-coated substrate, overview. TTL possesses broad substrate specificity and is active on triglycerides with various acyl chain lengths, phospholipids, and galactolipids Thermomyces dupontii ? - ? 89 3.1.1.3 olive oil + H2O - Thermomyces dupontii ? - ? 364063 3.1.1.3 phosphatidylcholine + H2O from hen egg Thermomyces dupontii ? - ? 64439 3.1.1.3 tributyrin + H2O - Thermomyces dupontii dibutyrin + butyrate - ? 363949 3.1.1.3 trioctanoin + H2O - Thermomyces dupontii dioctanoin + octanoate - ? 393712 3.1.1.3 additional information Talaromyces thermophilus lipase (TTL) also hydrolyzes monogalactosyl diacylglycerol (MGDG) and digalactosyl diacylglycerol (DGDG) substrates (EC 3.1.1.26) presented in various forms to the enzyme. The enzyme has galactolipase activity on galactolipid micelles, monomolecular films, and UV-absorbing surface-coated substrate, overview. TTL possesses broad substrate specificity and is active on triglycerides with various acyl chain lengths, phospholipids, and galactolipids Thermomyces dupontii Stolk ? - ? 89 3.1.1.3 olive oil + H2O - Thermomyces dupontii Stolk ? - ? 364063 3.1.1.3 phosphatidylcholine + H2O from hen egg Thermomyces dupontii Stolk ? - ? 64439 3.1.1.3 tributyrin + H2O - Thermomyces dupontii Stolk dibutyrin + butyrate - ? 363949 3.1.1.3 trioctanoin + H2O - Thermomyces dupontii Stolk dioctanoin + octanoate - ? 393712 3.1.1.3 1,2-dioleoylglycerol + H2O - Thermomyces lanuginosus oleic acid + ? - ? 64393 3.1.1.3 4-nitrophenyl butanoate + H2O - Thermomyces lanuginosus 4-nitrophenol + butanoate - ? 363828 3.1.1.3 4-nitrophenyl palmitate + H2O - Thermomyces lanuginosus 4-nitrophenol + palmitate - ? 363890 3.1.1.3 butanol + butanoate - Thermomyces lanuginosus butyl butanoate + H2O - ? 441152 3.1.1.3 ethanol + soybean oil transesterification reaction Thermomyces lanuginosus ? - ? 441370 3.1.1.3 methyl linoleate + H2O - Thermomyces lanuginosus methanol + linolic acid - ? 64427 3.1.1.3 methyl linolenate + H2O - Thermomyces lanuginosus methanol + linoleic acid - ? 64428 3.1.1.3 methyl oleate + H2O - Thermomyces lanuginosus methanol + oleic acid - ? 64426 3.1.1.3 additional information 1,3-positional specific lipase Thermomyces lanuginosus ? - ? 89 3.1.1.3 additional information enzyme does not possess protease activity and does not hydrolyze peptide bonds, no activity with N-benzoyl-L-tyrosine 4-nitroanilide Thermomyces lanuginosus ? - ? 89 3.1.1.3 palm olein + H2O 28% conversion in 6 h Thermomyces lanuginosus ? - ? 401612 3.1.1.3 polyethylene sorbitan monooleate + H2O i.e. Tween 80 Thermomyces lanuginosus ? - ? 64431 3.1.1.3 Span 85 + H2O - Thermomyces lanuginosus ? - ? 64432 3.1.1.3 tributyrin + H2O - Thermomyces lanuginosus dibutyrin + butyrate - ? 363949 3.1.1.3 trilaurin + H2O - Thermomyces lanuginosus lauric acid + ? - ? 64396 3.1.1.3 triolein + H2O - Thermomyces lanuginosus diolein + oleate - ? 363933 3.1.1.3 trioleoylglycerol + H2O - Thermomyces lanuginosus oleic acid + ? - ? 64389 3.1.1.3 1,2-dioleoylglycerol + H2O - Thermomyces lanuginosus No. 3 oleic acid + ? - ? 64393 3.1.1.3 methyl oleate + H2O - Thermomyces lanuginosus No. 3 methanol + oleic acid - ? 64426 3.1.1.3 additional information 1,3-positional specific lipase Thermomyces lanuginosus No. 3 ? - ? 89 3.1.1.3 trilaurin + H2O - Thermomyces lanuginosus No. 3 lauric acid + ? - ? 64396 3.1.1.3 trioleoylglycerol + H2O - Thermomyces lanuginosus No. 3 oleic acid + ? - ? 64389 3.1.1.3 4-nitrophenyl laurate + H2O - Thermosyntropha lipolytica 4-nitrophenol + laurate - ? 363891 3.1.1.3 4-nitrophenyl palmitate + H2O - Thermosyntropha lipolytica 4-nitrophenol + palmitate - ? 363890 3.1.1.3 additional information LipA and LipB exhibit high activity with long-chain fatty acid glycerides, yielding maximum activity with trioleate and, among the 4-nitrophenyl esters, with 4-nitrophenyl laurate, hydrolysis of glycerol ester bonds occurs at positions 1 and 3, tributyrin and tricaproin are poor substrates, overview Thermosyntropha lipolytica ? - ? 89 3.1.1.3 triolein + H2O - Thermosyntropha lipolytica diolein + oleate - ? 363933 3.1.1.3 4-nitrophenyl laurate + H2O - Thermus thermophilus 4-nitrophenol + laurate - ? 363891 3.1.1.3 4-nitrophenyl laurate + H2O - Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 4-nitrophenol + laurate - ? 363891 3.1.1.3 2,3-dimercapto-1-propanol tributyrate + H2O - Todarodes pacificus ? - ? 364145 3.1.1.3 olive oil + H2O - Trichoderma reesei ? - ? 364063 3.1.1.3 tributyrin + 3 H2O - Trichoderma reesei glycerol + 3 butyrate - ? 402043 3.1.1.3 vinyl butyrate + H2O - Trichoderma reesei ? - ? 398573 3.1.1.3 olive oil + H2O - Trichoderma reesei QM6a ? - ? 364063 3.1.1.3 tributyrin + 3 H2O - Trichoderma reesei QM6a glycerol + 3 butyrate - ? 402043 3.1.1.3 vinyl butyrate + H2O - Trichoderma reesei QM6a ? - ? 398573 3.1.1.3 additional information the enzyme is a hemolytic factor and involved in pathogenicity and as alternative source of iron from erythrocytes Trichomonas vaginalis ? - ? 89 3.1.1.3 4-nitrophenyl palmitate + H2O - Trichosporon asahii 4-nitrophenol + palmitate - ? 363890 3.1.1.3 additional information the enzyme reveals mid- to long- chain specificity for 4-nitrophenyl esters and triacylglycerols, with highest specificity on C-12 followed by C-14, C-16, and C-18. The enzyme shows (S)-selectivity in 1,4-dioxane and 2-propanol and (R)-selectivity in hexane during chiral separation of (+/-)1-phenylethanol by esterification Trichosporon asahii ? - ? 89 3.1.1.3 triacylglycerol + H2O - Trichosporon asahii diacylglycerol + a carboxylate - ? 360693 3.1.1.3 4-nitrophenyl palmitate + H2O - Trichosporon asahii MSR54 4-nitrophenol + palmitate - ? 363890 3.1.1.3 additional information the enzyme reveals mid- to long- chain specificity for 4-nitrophenyl esters and triacylglycerols, with highest specificity on C-12 followed by C-14, C-16, and C-18. The enzyme shows (S)-selectivity in 1,4-dioxane and 2-propanol and (R)-selectivity in hexane during chiral separation of (+/-)1-phenylethanol by esterification Trichosporon asahii MSR54 ? - ? 89 3.1.1.3 triacylglycerol + H2O - Trichosporon asahii MSR54 diacylglycerol + a carboxylate - ? 360693 3.1.1.3 (RS)-1-phenylethanol + vinyl acetate - Triticum aestivum (S)-1-phenylethyl acetate + acetaldehyde + (R)-1-phenylethanol - ? 399308 3.1.1.3 1-phenylethanol + vinyl butanoate - Triticum aestivum ? - ? 399309 3.1.1.3 1-phenylethanol + vinyl caproate - Triticum aestivum ? - ? 399310 3.1.1.3 1-phenylethanol + vinyl octanoate - Triticum aestivum ? - ? 399311 3.1.1.3 olive oil + H2O purified emulsion Triticum aestivum ? - ? 364063 3.1.1.3 1,2-di-O-lauryl-rac-glycero-3-glutaric acid 6'-methylresorufin ester + H2O chromogenic substrate uncultured bacterium ? - ? 390484 3.1.1.3 4-nitrophenyl caprylate + H2O - uncultured bacterium 4-nitrophenol + caprylate - ? 363889 3.1.1.3 4-nitrophenyl decanoate + H2O - uncultured bacterium 4-nitrophenol + decanoate - ? 360778 3.1.1.3 4-nitrophenyl decanoate + H2O about 25% activity compared to 4-nitrophenyl laurate uncultured bacterium 4-nitrophenol + decanoate - ? 360778 3.1.1.3 4-nitrophenyl laurate + H2O - uncultured bacterium 4-nitrophenol + laurate - ? 363891 3.1.1.3 4-nitrophenyl laurate + H2O highest activity (100%) uncultured bacterium 4-nitrophenol + laurate - ? 363891 3.1.1.3 4-nitrophenyl myristate + H2O - uncultured bacterium 4-nitrophenol + myristate - ? 364082 3.1.1.3 4-nitrophenyl myristate + H2O about 50% activity compared to 4-nitrophenyl laurate uncultured bacterium 4-nitrophenol + myristate - ? 364082 3.1.1.3 4-nitrophenyl octanoate + H2O about 3% activity compared to 4-nitrophenyl laurate uncultured bacterium 4-nitrophenol + octanoate - ? 115380 3.1.1.3 4-nitrophenyl palmitate + H2O about 18% activity compared to 4-nitrophenyl laurate uncultured bacterium 4-nitrophenol + palmitate - ? 363890 3.1.1.3 4-nitrophenyl stearate + H2O about 6% activity compared to 4-nitrophenyl laurate uncultured bacterium 4-nitrophenol + stearate - ? 364135 3.1.1.3 4-nitrophenylbutyrate + H2O - uncultured bacterium 4-nitrophenol + butyrate - ? 383927 3.1.1.3 additional information the lipase hydrolyses 4-nitrophenyl esters of fatty acids with chain lengths up to C14 uncultured bacterium ? - ? 89 3.1.1.3 additional information no activity with 4-nitrophenyl butyrate and 4-nitrophenyl propionate uncultured bacterium ? - ? 89 3.1.1.3 tributyrin + H2O - uncultured bacterium dibutyrin + butyrate - ? 363949 3.1.1.3 4-nitrophenyl acetate + H2O - Weizmannia coagulans 4-nitrophenol + acetate - ? 12514 3.1.1.3 4-nitrophenyl caprylate + H2O - Weizmannia coagulans 4-nitrophenol + caprylate - ? 363889 3.1.1.3 4-nitrophenyl formate + H2O - Weizmannia coagulans 4-nitrophenol + formate - ? 381192 3.1.1.3 4-nitrophenyl laurate + H2O - Weizmannia coagulans 4-nitrophenol + laurate - ? 363891 3.1.1.3 4-nitrophenyl octanoate + H2O - Weizmannia coagulans 4-nitrophenol + octanoate - ? 115380 3.1.1.3 4-nitrophenyl palmitate + H2O - Weizmannia coagulans 4-nitrophenol + palmitate - ? 363890 3.1.1.3 4-nitrophenyl palmitate + H2O best substrate Weizmannia coagulans 4-nitrophenol + palmitate - ? 363890 3.1.1.3 additional information substrate specificity, the enzyme shows preference for triacylglyceride substrates with long fatty acid chains Weizmannia coagulans ? - ? 89 3.1.1.3 4-nitrophenyl acetate + H2O - Weizmannia coagulans BTS-3 4-nitrophenol + acetate - ? 12514 3.1.1.3 4-nitrophenyl caprylate + H2O - Weizmannia coagulans BTS-3 4-nitrophenol + caprylate - ? 363889 3.1.1.3 4-nitrophenyl laurate + H2O - Weizmannia coagulans BTS-3 4-nitrophenol + laurate - ? 363891 3.1.1.3 4-nitrophenyl palmitate + H2O best substrate Weizmannia coagulans BTS-3 4-nitrophenol + palmitate - ? 363890 3.1.1.3 additional information substrate specificity, the enzyme shows preference for triacylglyceride substrates with long fatty acid chains Weizmannia coagulans BTS-3 ? - ? 89 3.1.1.3 4-nitrophenyl octanoate + H2O - Weizmannia coagulans MTCC-6375 4-nitrophenol + octanoate - ? 115380 3.1.1.3 4-nitrophenyl palmitate + H2O - Weizmannia coagulans MTCC-6375 4-nitrophenol + palmitate - ? 363890 3.1.1.3 2 triolein + H2O 1,3-specificity for triolein Yarrowia lipolytica 3 oleic acid + 2-monoolein + 1,3-diolein - ? 417724 3.1.1.3 4-nitrophenyl acetate + H2O about 34% activity compared to 4-nitrophenyl decanoate Yarrowia lipolytica 4-nitrophenol + acetate - ? 12514 3.1.1.3 4-nitrophenyl butyrate + H2O about 42% activity compared to 4-nitrophenyl butyrate Yarrowia lipolytica 4-nitrophenol + butyrate - ? 115376 3.1.1.3 4-nitrophenyl caproate + H2O low activity Yarrowia lipolytica 4-nitrophenol + caproate - ? 363887 3.1.1.3 4-nitrophenyl caprylate + H2O about 70% activity compared to 4-nitrophenyl decanoate Yarrowia lipolytica 4-nitrophenol + caprylate - ? 363889 3.1.1.3 4-nitrophenyl decanoate + H2O 100% activity Yarrowia lipolytica 4-nitrophenol + decanoate - ? 360778 3.1.1.3 4-nitrophenyl hexanoate + H2O about 40% activity compared to 4-nitrophenyl decanoate Yarrowia lipolytica 4-nitrophenol + hexanoate - ? 115379 3.1.1.3 4-nitrophenyl laurate + H2O - Yarrowia lipolytica 4-nitrophenol + laurate - ? 363891 3.1.1.3 4-nitrophenyl laurate + H2O about 65% activity compared to 4-nitrophenyl decanoate Yarrowia lipolytica 4-nitrophenol + laurate - ? 363891 3.1.1.3 4-nitrophenyl myristate + H2O about 10% activity compared to 4-nitrophenyl decanoate Yarrowia lipolytica 4-nitrophenol + myristate - ? 364082 3.1.1.3 4-nitrophenyl oleate + H2O about 3% activity compared to 4-nitrophenyl decanoate Yarrowia lipolytica 4-nitrophenol + oleate - ? 364073 3.1.1.3 4-nitrophenyl palmitate + H2O - Yarrowia lipolytica 4-nitrophenol + palmitate - ? 363890 3.1.1.3 4-nitrophenyl palmitate + H2O high activity Yarrowia lipolytica 4-nitrophenol + palmitate - ? 363890 3.1.1.3 4-nitrophenyl palmitate + H2O about 8% activity compared to 4-nitrophenyl decanoate Yarrowia lipolytica 4-nitrophenol + palmitate - ? 363890 3.1.1.3 amla oil + H2O - Yarrowia lipolytica ? - ? 429891 3.1.1.3 cotton seed oil + H2O - Yarrowia lipolytica ? - ? 391692 3.1.1.3 flax seed oil + H2O - Yarrowia lipolytica ? - ? 391984 3.1.1.3 groundnut oil + H2O - Yarrowia lipolytica ? - ? 364068 3.1.1.3 methyl myristate + H2O best fatty acid methyl ester substrate Yarrowia lipolytica methanol + myristate - ? 364187 3.1.1.3 additional information substrate specificity of LipY8p, overview Yarrowia lipolytica ? - ? 89 3.1.1.3 additional information the lipase showed a preference for long chain fatty acid methyl esters of C12-C16, substrate specificity, overview Yarrowia lipolytica ? - ? 89 3.1.1.3 additional information the lipase showed high activity toward long-chain fatty acid methyl esters with C12–C16, overview Yarrowia lipolytica ? - ? 89 3.1.1.3 additional information no phospholipid-hydrolyzing activity is detected with Lip9 Yarrowia lipolytica ? - ? 89 3.1.1.3 olive oil + H2O - Yarrowia lipolytica ? - ? 364063 3.1.1.3 olive oil + H2O best substrate Yarrowia lipolytica ? - ? 364063 3.1.1.3 safflower oil + H2O - Yarrowia lipolytica ? - ? 393457 3.1.1.3 soybean oil + H2O - Yarrowia lipolytica ? - ? 364064 3.1.1.3 soybean oil + H2O substrate is stabilized with egg yolk lecithin Yarrowia lipolytica ? - ? 364064 3.1.1.3 tributyrin + H2O - Yarrowia lipolytica ? - ? 64455 3.1.1.3 tributyrin + H2O - Yarrowia lipolytica dibutyrin + butyrate - ? 363949 3.1.1.3 tricaprylin + H2O - Yarrowia lipolytica dicaprylin + caprylate - ? 364128 3.1.1.3 tridecanoate + H2O - Yarrowia lipolytica ? - ? 430739 3.1.1.3 trilaurin + H2O low activity Yarrowia lipolytica dilaurin + laurate - ? 364081 3.1.1.3 trilaurin + H2O - Yarrowia lipolytica ? - ? 417719 3.1.1.3 trimyristin + H2O - Yarrowia lipolytica ? - ? 430740 3.1.1.3 trioctanoin + H2O - Yarrowia lipolytica ? - ? 64460 3.1.1.3 triolein + H2O - Yarrowia lipolytica ? - ? 64454 3.1.1.3 triolein + H2O the enzyme shows a preference for the hydrolysis of the sn-3 ester bond of triolein compared to the sn-1 position Yarrowia lipolytica 1,2-diolein + oleate higher amount of 1,2-diolein ? 393714 3.1.1.3 tripalmitin + H2O low activity Yarrowia lipolytica dipalmitin + palmitate - ? 364080 3.1.1.3 tripalmitin + H2O - Yarrowia lipolytica ? - ? 413001 3.1.1.3 tristearin + H2O low activity Yarrowia lipolytica distearin + stearate - ? 364134 3.1.1.3 tristearin + H2O - Yarrowia lipolytica ? - ? 413004 3.1.1.3 4-nitrophenyl laurate + H2O - Yarrowia lipolytica AS 2.1216 4-nitrophenol + laurate - ? 363891 3.1.1.3 additional information substrate specificity of LipY8p, overview Yarrowia lipolytica AS 2.1216 ? - ? 89 3.1.1.3 4-nitrophenyl acetate + H2O about 34% activity compared to 4-nitrophenyl decanoate Yarrowia lipolytica CLIB 122 4-nitrophenol + acetate - ? 12514 3.1.1.3 4-nitrophenyl butyrate + H2O about 42% activity compared to 4-nitrophenyl butyrate Yarrowia lipolytica CLIB 122 4-nitrophenol + butyrate - ? 115376 3.1.1.3 4-nitrophenyl decanoate + H2O 100% activity Yarrowia lipolytica CLIB 122 4-nitrophenol + decanoate - ? 360778 3.1.1.3 4-nitrophenyl hexanoate + H2O about 40% activity compared to 4-nitrophenyl decanoate Yarrowia lipolytica CLIB 122 4-nitrophenol + hexanoate - ? 115379 3.1.1.3 additional information no phospholipid-hydrolyzing activity is detected with Lip9 Yarrowia lipolytica CLIB 122 ? - ? 89 3.1.1.3 4-nitrophenyl caproate + H2O low activity Yarrowia lipolytica MSR80 4-nitrophenol + caproate - ? 363887 3.1.1.3 4-nitrophenyl palmitate + H2O high activity Yarrowia lipolytica MSR80 4-nitrophenol + palmitate - ? 363890 3.1.1.3 cotton seed oil + H2O - Yarrowia lipolytica MSR80 ? - ? 391692 3.1.1.3 groundnut oil + H2O - Yarrowia lipolytica MSR80 ? - ? 364068 3.1.1.3 triolein + H2O - Yarrowia lipolytica MSR80 ? - ? 64454