2.7.8.31 additional information the enzyme catalyzes the initial step of the xanthan biosynthesis that transfers a glucose-phosphate residue from UDP-glucose to an isoprenoid lipid carrier Xanthomonas campestris pv. campestris ? - ? 89 2.7.8.31 additional information the enzyme catalyzes the initial step of the xanthan biosynthesis that transfers a glucose-phosphate residue from UDP-glucose to an isoprenoid lipid carrier Xanthomonas campestris pv. campestris B100 ? - ? 89 2.7.8.31 UDP-alpha-D-galactose + undecaprenyl phosphate - Escherichia coli UMP + alpha-D-galactosyl-diphosphoundecaprenol - ? 424153 2.7.8.31 UDP-alpha-D-galactose + undecaprenyl phosphate - Caulobacter vibrioides UMP + alpha-D-galactosyl-diphosphoundecaprenol - ? 424153 2.7.8.31 UDP-alpha-D-galactose + undecaprenyl phosphate - Caulobacter vibrioides CB15 UMP + alpha-D-galactosyl-diphosphoundecaprenol - ? 424153 2.7.8.31 UDP-alpha-D-glucose + undecaprenyl phosphate UDP-alpha-D-glucose is the preferred substrate Escherichia coli UMP + alpha-D-glucosyl-diphosphoundecaprenol - ? 424180 2.7.8.31 UDP-alpha-D-glucose + undecaprenyl phosphate UDP-alpha-D-glucose is the preferred substrate Caulobacter vibrioides UMP + alpha-D-glucosyl-diphosphoundecaprenol - ? 424180 2.7.8.31 UDP-alpha-D-glucose + undecaprenyl phosphate UDP-alpha-D-glucose is the preferred substrate Caulobacter vibrioides CB15 UMP + alpha-D-glucosyl-diphosphoundecaprenol - ? 424180 2.7.8.31 UDP-glucose + ditrans,octacis-undecaprenyl phosphate GumD is involved in biosynthesis of the pentasaccharide repeating unit of xanthan. It is suggested that the wild-type Xanthomonas oryzae-produced xanthan is assembled by the sequential addition of UDP-glucose, UDP-glucose, GDP-mannose, UDPglucuronic acid, and GDP-mannose onto a polyprenol phosphate carrier, by the glycosyltransferase homologues encoded by the gumD, gumM, gumH, gumK, and gumI genes, respectively Xanthomonas oryzae UMP + alpha-D-glucopyranosyl-diphospho-ditrans,octacis-undecaprenol - ? 407434 2.7.8.31 UDP-glucose + ditrans,octacis-undecaprenyl phosphate the enzyme is involved in biosynthesis of xanthan Xanthomonas campestris UMP + alpha-D-glucopyranosyl-diphospho-ditrans,octacis-undecaprenol - ? 407434 2.7.8.31 UDP-glucose + ditrans,octacis-undecaprenyl phosphate EDTA-treated Xanthomonas campestris cells are used as both enzyme preparation and lipid-P acceptor, and UDP-Glc, GDP-Man, and UDP-glucuronic acid are used as sugar donors. A linear pentasaccharide unit is assembled on a polyprenol-P lipid carrier by the sequential addition of glucose-1-P, glucose, mannose, glucuronic acid, and mannose Xanthomonas campestris UMP + alpha-D-glucopyranosyl-diphospho-ditrans,octacis-undecaprenol - ? 407434 2.7.8.31 UDP-glucose + ditrans,octacis-undecaprenyl phosphate the C-terminal domain of the gumD gene product is sufficient for its glucosyl-1-phosphate transferase activity Xanthomonas campestris UMP + alpha-D-glucopyranosyl-diphospho-ditrans,octacis-undecaprenol - ? 407434