2.7.7.B22	additional information	formation of a Hermes transposase-DNA complex. Bipartite DNA recognition at hAT transposon ends and Hermes-DNA interactions within the transpososome. No protein-DNA interactions involving bp 12-16 of the TIR. The avidity provided by multiple sites of interaction allows a transposase to locate its transposon ends amidst a sea of chromosomal DNA, mechanism, overview. The enzyme possesses a RNaseH-like catalytic domain interrupted by a large [.alpha]-helical insertion domain, and an N-terminal intertwined dimerization domain. Together, these domains catalyze the chemical steps of DNA nicking, hairpin formation, and DNA strand transfer that comprise hAT transposition	Musca domestica	?	-	?	89	
2.7.7.B22	additional information	H2K A/J muscle cells properly express full-length human DYSF following SB-mediated gene transfer. A duplicate of Spc5-12 (2xSpc5-12) regulatory sequence proves to be the most efficient in driving transgene expression in H2K A/J myoblasts. Corrected H2K A/J myoblasts can efficiently be transplanted into Scid/BLA/J mouse muscle	Homo sapiens	?	-	?	89	
2.7.7.B22	additional information	the sleeping beauty transposon DNA contains the gene to be inserted into the target DNA flanked by inverted terminal repeats (IR-DRleft and IR-DRright). Each inverted terminal repeat contains two, inner and outer, direct repeats (DRs). The DRs represent binding sites for the transposase enzyme. Sleeping beauty, SB, appears to be the most random in preferences for integration sites, requiring only a TA dinucleotide basepair. Binding mechanism, detailed overview	Homo sapiens	?	-	?	89	
2.7.7.B22	additional information	for examination of the transposition activity of recombinant Tgf2 TPase, microinjection is performed in blunt snout bream embryos at 1-2 cell stage. When donor plasmid pTgf2-EF1alpha-EGFP is co-injected with recombinant Tgf2 TPase protein, 74% of the embryos show almost ubiquitous and uniform expression of EGFP, while 31% control embryos by injected donor plasmid show only mosaic expression of EGFP. Four EGFP-positive blunt snout bream are sampled from the group coinjected with donor plasmid with recombinant Tgf2 TPase. An 8-bp direct repeat of target DNA at the integration site, the signature of hAT family transposons, is created adjacent to both ends of Tgf2 at the integration sites in all fish, indicating the recombinant Tgf2 TPase insertions occur by transposition	Carassius auratus	?	-	?	89	
2.7.7.B22	additional information	identification of multiple DNA binding sites for the THAP domain of the Galileo transposase in the long terminal inverted-repeats, these TIRs and other Foldback-like elements may provide the transposase with its binding specificity	Drosophila buzzatii	?	-	?	89	
2.7.7.B22	additional information	interaction analysis of full-length enzyme and of PAI subdomain with transposon DNA, overview	Homo sapiens	?	-	?	89	
2.7.7.B22	additional information	Sleeping Beauty transposase excision activity is quantified using a fluorescence-activated cell sorting (FACS)-based excision assay in HeLa cells, overall SB transposition activity is also detected in HeLa cells	Homo sapiens	?	-	?	89	
2.7.7.B22	additional information	Tgf2 is an autonomously active vertebrate transposon that is efficient at gene-transfer in teleost fish. The N-terminal zinc finger domain of Tgf2 transposase contributes to DNA binding and to transposition activity. Proposed model for Tgf2 transposition, overview	Carassius auratus	?	-	?	89	
2.7.7.B22	additional information	The reaction of DNA transposition begins when the transposase enzyme binds to the transposon DNA. Folding of the specific DNA recognition subdomain of the sleeping beauty transposase is temperature-dependent and is required for its binding to the transposon DNA. Only the folded conformation of the specific DNA recognition subdomain of the Sleeping Beauty transposase, the PAI subdomain, binds to the transposon DNA. The PAI subdomain is well folded at low temperatures, but the presence of unfolded conformation gradually increases at temperatures above 15°C. DNA-binding of folded and unfolded conformations of the PAI subdomain, overview	Homo sapiens	?	-	?	89	
2.7.7.B22	additional information	the TnpA transposase precisely cleaves leaft and right ends (LE and RE) of a gene without leaving behind a scar sequence. Development of a simple and precise method for genome manipulation in Escherichia coli that alters the gene sequence without leaving foreign DNA in the chromosome. This strategy involves PCR amplification of a DNA cassette containing ISHp608-LE (left end)-antibiotic resistance gene-counterselection marker-ISHp608-RE (right end) by using primers containing extensions homologous to the adjacent regions of the target gene on the chromosome. The lambda Red-mediated recombination of the PCR product and antibiotic resistance screening results in transformants with a modified gene target. The ISHp608-LE-antibiotic resistance gene-counterselection marker-ISHp608-RE cassette can then be excised using a temperature sensitive plasmid expressing the TnpA transposase, which precisely cleaves ISHp608-LE and ISHp608-RE without leaving a scar sequence. For introduction of IS608 LE and RE into the gene of interest, lambda-Red recombination is utilized, which does not require in vitro manipulations such as restriction digestion, ligation or construction of a suicide vector. Diagram of plasmids containing selectable and excisable IS608 cassettes, overview	Escherichia coli O103:H2	?	-	?	89	
2.7.7.B22	additional information	the transposase encoded by Acidiphilium binds to the archaeal sliding clamp (PCNA) of Methanosarcina and to the beta sliding clamp of Acidiphilium, Leptospirillum, and Escherichia coli	Acidiphilium sp. PM	?	-	?	89	
2.7.7.B22	additional information	the transposase encoded by Methanosarcina IS1634 binds to the sliding clamp of Acidiphilium beta	Methanosarcina barkeri	?	-	?	89	
2.7.7.B22	additional information	incubation of Kat1 and a 197 bp duplex corresponding to TIR-R with 48 bp from the transposon and 149 bp flanking DNA. First Kat1 cleaves the transposon end, generating a free 3' hydroxyl. Joining of one 3'-OH to the target generates a nicked plasmid or single end-joining (SEJ) product. If two 3'-OHs join the target at complementary positions on opposite strands, the plasmid is linearized generating a double end-joining (DEJ) product. Kat1 displays target joining, generating both SEJ- and DEJ-products	Kluyveromyces lactis	?	-	-	89	
2.7.7.B22	additional information	incubation of Kat1 with a 40 bp duplex representing imperfect inverted repeat TIR-R and including 30 bp from the transposon end and 10 bp from the flanking DNA. Kat1 cleaves the upper strand primarily at GTATA*C and to less extent at GTAT*AC. In the reaction using 3' end-labeling of the upper strand a product of 23 nts is observed. The products formed from the lower strand are hairpin-capped. No substrate: a 40 bp duplex representing imperfect inverted repeat TIR-L	Kluyveromyces lactis	?	-	-	89	
2.7.7.B22	additional information	the transposase encoded by Methanosarcina IS1634 binds to the sliding clamp of Acidiphilium beta	Methanosarcina barkeri DSM 804	?	-	?	89	
2.7.7.B22	additional information	the TnpA transposase precisely cleaves leaft and right ends (LE and RE) of a gene without leaving behind a scar sequence. Development of a simple and precise method for genome manipulation in Escherichia coli that alters the gene sequence without leaving foreign DNA in the chromosome. This strategy involves PCR amplification of a DNA cassette containing ISHp608-LE (left end)-antibiotic resistance gene-counterselection marker-ISHp608-RE (right end) by using primers containing extensions homologous to the adjacent regions of the target gene on the chromosome. The lambda Red-mediated recombination of the PCR product and antibiotic resistance screening results in transformants with a modified gene target. The ISHp608-LE-antibiotic resistance gene-counterselection marker-ISHp608-RE cassette can then be excised using a temperature sensitive plasmid expressing the TnpA transposase, which precisely cleaves ISHp608-LE and ISHp608-RE without leaving a scar sequence. For introduction of IS608 LE and RE into the gene of interest, lambda-Red recombination is utilized, which does not require in vitro manipulations such as restriction digestion, ligation or construction of a suicide vector. Diagram of plasmids containing selectable and excisable IS608 cassettes, overview	Escherichia coli O103:H2 EHEC	?	-	?	89