2.4.1.9 3 sucrose + sucrose - Aureobasidium sp. 3 alpha-D-glucose + (2,1-beta-D-fructosyl)n+1 - ? 389832 2.4.1.9 3 sucrose + sucrose enzyme produces a diverse range of fructooligosaccharide molecules and a minor amount of inulin polymer [with beta(2–1) linkages] Limosilactobacillus reuteri 3 alpha-D-glucose + (2,1-beta-D-fructosyl)n+1 - ? 389832 2.4.1.9 3 sucrose + sucrose enzyme produces a diverse range of fructooligosaccharide molecules and a minor amount of inulin polymer [with beta(2–1) linkages] Limosilactobacillus reuteri 121 3 alpha-D-glucose + (2,1-beta-D-fructosyl)n+1 - ? 389832 2.4.1.9 additional information the enzyme is able to catalyse a disproportionation type of reaction with 1-kestose, 1,1-nystose and 1,1,1-kestopentaose Limosilactobacillus reuteri ? - ? 89 2.4.1.9 additional information the production of fructooligosaccharide by this enzyme is possible only in the presence of sucrose which is an exclusive donor of fructosyl residue in the transferase reaction. The acceptor can be another molecule of sucrose or frucotoligosaccharide Aureobasidium pullulans ? - ? 89 2.4.1.9 additional information wild-type inulosucrase synthesizes mostly fructooligosaccharides up to a degree of polymerization of 15 and relatively low amounts of inulin polymer Limosilactobacillus reuteri ? - ? 89 2.4.1.9 additional information very low activity in acceptor reactions with non-sugar acceptors, specificity analysis, overview Leuconostoc citreum ? - ? 89 2.4.1.9 additional information synthesis of 1-kestose and nystose Leuconostoc citreum ? - ? 89 2.4.1.9 additional information enzymatic synthesis of maltosylfructosides derived from sucrose-maltose mixtures by the enzyme from strain DSM 20604, structure analysis, and relation to fructooligosaccharides contents, overview Lactobacillus gasseri ? - ? 89 2.4.1.9 additional information sucrose or raffinose act as substrates, both donor and acceptor of fructose moieties. Sucrose is the preferred substrate. The enzyme synthezises fructooligosaccharides from degree of polymerization of 3 (1-kestose) to 9, as well as minor amounts of neo-kestose and inulobiose, product analysis by NMR spectroscopy, overview. Synthezised oligomers are identified as alpha-D-galactopyranosyl-(1->6)-alpha-D-glucopyranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranoside, alpha-D-galactopyranosyl-(1->6)-alpha-D-glucopyranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranoside, alpha-D-galactopyranosyl-(1->6)-alpha-D-glucopyranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranoside, alpha-D-galactopyranosyl-(1->6)-alpha-D-glucopyranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranoside, and alpha-D-galactopyranosyl-(1->6)-alpha-D-glucopyranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranoside Lactobacillus gasseri ? - ? 89 2.4.1.9 additional information the truncated enzyme mutant IslA4 can synthesize high molecular weight inulin from sucrose, with a residual sucrose hydrolytic activity, and a product specificity similar to the multidomain wild-type enzyme. High sucrose concentrations shift the specificity of the reaction towards fructooligosaccharides (FOS) synthesis, which almost eliminates inulin synthesis and leads to a considerable reduction in sucrose hydrolysis. Reactions with low IslA4 activity and a high sucrose activity allow for high levels of FOS synthesis, where 70% sucrose is used for transfer reactions, with 65% corresponding to transfructosylation for the synthesis of FOS, quantitative product analysis, overview Leuconostoc citreum ? - ? 89 2.4.1.9 additional information the enzyme also hydrolyzes sucrose to D-fructose and D-glucose Limosilactobacillus reuteri ? - - 89 2.4.1.9 additional information the enzyme also hydrolyzes sucrose to D-fructose and D-glucose Limosilactobacillus reuteri 121 ? - - 89 2.4.1.9 additional information wild-type inulosucrase synthesizes mostly fructooligosaccharides up to a degree of polymerization of 15 and relatively low amounts of inulin polymer Limosilactobacillus reuteri 121 ? - ? 89 2.4.1.9 additional information the enzyme is able to catalyse a disproportionation type of reaction with 1-kestose, 1,1-nystose and 1,1,1-kestopentaose Limosilactobacillus reuteri 121 ? - ? 89 2.4.1.9 additional information sucrose or raffinose act as substrates, both donor and acceptor of fructose moieties. Sucrose is the preferred substrate. The enzyme synthezises fructooligosaccharides from degree of polymerization of 3 (1-kestose) to 9, as well as minor amounts of neo-kestose and inulobiose, product analysis by NMR spectroscopy, overview. Synthezised oligomers are identified as alpha-D-galactopyranosyl-(1->6)-alpha-D-glucopyranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranoside, alpha-D-galactopyranosyl-(1->6)-alpha-D-glucopyranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranoside, alpha-D-galactopyranosyl-(1->6)-alpha-D-glucopyranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranoside, alpha-D-galactopyranosyl-(1->6)-alpha-D-glucopyranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranoside, and alpha-D-galactopyranosyl-(1->6)-alpha-D-glucopyranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranoside Lactobacillus gasseri DSM 20604 ? - ? 89 2.4.1.9 additional information enzymatic synthesis of maltosylfructosides derived from sucrose-maltose mixtures by the enzyme from strain DSM 20604, structure analysis, and relation to fructooligosaccharides contents, overview Lactobacillus gasseri DSM 20604 ? - ? 89 2.4.1.9 raffinose + [(2->1)-beta-D-fructosyl]n - Lactobacillus gasseri glucose + [(2->1)-beta-D-fructosyl]n+1 - ? 435784 2.4.1.9 raffinose + [(2->1)-beta-D-fructosyl]n - Lactobacillus gasseri DSM 20604 glucose + [(2->1)-beta-D-fructosyl]n+1 - ? 435784 2.4.1.9 sucrose + (2,1-beta-D-fructosyl)n - Limosilactobacillus reuteri alpha-D-glucose + (2,1-beta-D-fructosyl)n+1 - ? 375307 2.4.1.9 sucrose + (2,1-beta-D-fructosyl)n - Leuconostoc citreum alpha-D-glucose + (2,1-beta-D-fructosyl)n+1 - ? 375307 2.4.1.9 sucrose + (2,1-beta-D-fructosyl)n transferase activity Limosilactobacillus reuteri alpha-D-glucose + (2,1-beta-D-fructosyl)n+1 i.e. inulin ? 375307 2.4.1.9 sucrose + (2,1-beta-D-fructosyl)n transferase activity Leuconostoc citreum alpha-D-glucose + (2,1-beta-D-fructosyl)n+1 i.e. inulin, determination of inulin product chain length, overview ? 375307 2.4.1.9 sucrose + (2,1-beta-D-fructosyl)n FTase possesses only transfructosylating activity, acts on sucrose by cleaving the beta-1,2 linkage and transferring the fructosyl group to an acceptor molecule such as sucrose and fructooligosaccharides, releasing D-glucose Aspergillus niger alpha-D-glucose + (2,1-beta-D-fructosyl)n+1 - ? 375307 2.4.1.9 sucrose + (2,1-beta-D-fructosyl)n FTase possesses only transfructosylating activity, acts on sucrose by cleaving the beta-1,2 linkage and transferring the fructosyl group to an acceptor molecule such as sucrose and fructooligosaccharides, releasing D-glucose Paenibacillus macerans alpha-D-glucose + (2,1-beta-D-fructosyl)n+1 - ? 375307 2.4.1.9 sucrose + (2,1-beta-D-fructosyl)n FTase possesses only transfructosylating activity, acts on sucrose by cleaving the beta-1,2 linkage and transferring the fructosyl group to an acceptor molecule such as sucrose and fructooligosaccharides, releasing D-glucose Talaromyces purpureogenus alpha-D-glucose + (2,1-beta-D-fructosyl)n+1 - ? 375307 2.4.1.9 sucrose + (2,1-beta-D-fructosyl)n FTase possesses only transfructosylating activity, acts on sucrose by cleaving the beta-1,2 linkage and transferring the fructosyl group to an acceptor molecule such as sucrose and fructooligosaccharides, releasing D-glucose Aspergillus oryzae alpha-D-glucose + (2,1-beta-D-fructosyl)n+1 - ? 375307 2.4.1.9 sucrose + (2,1-beta-D-fructosyl)n FTase possesses only transfructosylating activity, acts on sucrose by cleaving the beta-1,2 linkage and transferring the fructosyl group to an acceptor molecule such as sucrose and fructooligosaccharides, releasing D-glucose Penicillium citrinum alpha-D-glucose + (2,1-beta-D-fructosyl)n+1 - ? 375307 2.4.1.9 sucrose + (2,1-beta-D-fructosyl)n FTase possesses only transfructosylating activity, acts on sucrose by cleaving the beta-1,2 linkage and transferring the fructosyl group to an acceptor molecule such as sucrose and fructooligosaccharides, releasing D-glucose Aspergillus japonicus alpha-D-glucose + (2,1-beta-D-fructosyl)n+1 - ? 375307 2.4.1.9 sucrose + (2,1-beta-D-fructosyl)n FTase possesses only transfructosylating activity, acts on sucrose by cleaving the beta-1,2 linkage and transferring the fructosyl group to an acceptor molecule such as sucrose and fructooligosaccharides, releasing D-glucose Aureobasidium pullulans alpha-D-glucose + (2,1-beta-D-fructosyl)n+1 - ? 375307 2.4.1.9 sucrose + (2,1-beta-D-fructosyl)n FTase possesses only transfructosylating activity, acts on sucrose by cleaving the beta-1,2 linkage and transferring the fructosyl group to an acceptor molecule such as sucrose and fructooligosaccharides, releasing D-glucose Thermothelomyces heterothallicus alpha-D-glucose + (2,1-beta-D-fructosyl)n+1 - ? 375307 2.4.1.9 sucrose + (2,1-beta-D-fructosyl)n FTase possesses only transfructosylating activity, acts on sucrose by cleaving the beta-1,2 linkage and transferring the fructosyl group to an acceptor molecule such as sucrose and fructooligosaccharides, releasing D-glucose Aureobasidium pullulans KCCM12017 alpha-D-glucose + (2,1-beta-D-fructosyl)n+1 - ? 375307 2.4.1.9 sucrose + (2,1-beta-D-fructosyl)n FTase possesses only transfructosylating activity, acts on sucrose by cleaving the beta-1,2 linkage and transferring the fructosyl group to an acceptor molecule such as sucrose and fructooligosaccharides, releasing D-glucose Aspergillus japonicus JN19 alpha-D-glucose + (2,1-beta-D-fructosyl)n+1 - ? 375307 2.4.1.9 sucrose + (2,1-beta-D-fructosyl)n FTase possesses only transfructosylating activity, acts on sucrose by cleaving the beta-1,2 linkage and transferring the fructosyl group to an acceptor molecule such as sucrose and fructooligosaccharides, releasing D-glucose Aspergillus oryzae CRF 202 alpha-D-glucose + (2,1-beta-D-fructosyl)n+1 - ? 375307 2.4.1.9 sucrose + (2,1-beta-D-fructosyl)n FTase possesses only transfructosylating activity, acts on sucrose by cleaving the beta-1,2 linkage and transferring the fructosyl group to an acceptor molecule such as sucrose and fructooligosaccharides, releasing D-glucose Paenibacillus macerans EG-6 alpha-D-glucose + (2,1-beta-D-fructosyl)n+1 - ? 375307 2.4.1.9 sucrose + (2,1-beta-D-fructosyl)n FTase possesses only transfructosylating activity, acts on sucrose by cleaving the beta-1,2 linkage and transferring the fructosyl group to an acceptor molecule such as sucrose and fructooligosaccharides, releasing D-glucose Aureobasidium pullulans CFR77 alpha-D-glucose + (2,1-beta-D-fructosyl)n+1 - ? 375307 2.4.1.9 sucrose + (2,1-beta-D-fructosyl)n - Leuconostoc citreum CW28 alpha-D-glucose + (2,1-beta-D-fructosyl)n+1 - ? 375307 2.4.1.9 sucrose + (2,1-beta-D-fructosyl)n transferase activity Leuconostoc citreum CW28 alpha-D-glucose + (2,1-beta-D-fructosyl)n+1 i.e. inulin, determination of inulin product chain length, overview ? 375307 2.4.1.9 sucrose + (2,1-beta-D-fructosyl)n FTase possesses only transfructosylating activity, acts on sucrose by cleaving the beta-1,2 linkage and transferring the fructosyl group to an acceptor molecule such as sucrose and fructooligosaccharides, releasing D-glucose Penicillium citrinum FERM P-15944 alpha-D-glucose + (2,1-beta-D-fructosyl)n+1 - ? 375307 2.4.1.9 sucrose + (2,1-beta-D-fructosyl)n - Limosilactobacillus reuteri 121 alpha-D-glucose + (2,1-beta-D-fructosyl)n+1 - ? 375307 2.4.1.9 sucrose + (2,1-beta-D-fructosyl)n transferase activity Limosilactobacillus reuteri 121 alpha-D-glucose + (2,1-beta-D-fructosyl)n+1 i.e. inulin ? 375307 2.4.1.9 sucrose + (2,1-beta-D-fructosyl)n - Limosilactobacillus reuteri D-glucose + (2,1-beta-D-fructosyl)n+1 - ? 375308 2.4.1.9 sucrose + (2,1-beta-D-fructosyl)n - Limosilactobacillus reuteri 121 D-glucose + (2,1-beta-D-fructosyl)n+1 - ? 375308 2.4.1.9 sucrose + H2O at sucrose concentrations lower than 200 mM (at 37°C), hydrolysis is the main enzyme activity. At higher sucrose concentrations, transglycosylation increases gradually, reaching 90% or more of total enzyme activity at 1.7 M sucrose Limosilactobacillus reuteri D-fructose + D-glucose - ? 94154 2.4.1.9 sucrose + H2O at sucrose concentrations lower than 200 mM (at 37°C), hydrolysis is the main enzyme activity. At higher sucrose concentrations, transglycosylation increases gradually, reaching 90% or more of total enzyme activity at 1.7 M sucrose Limosilactobacillus reuteri 121 D-fructose + D-glucose - ? 94154 2.4.1.9 sucrose + H2O - Leuconostoc citreum alpha-D-glucose + beta-D-fructose - ? 374674 2.4.1.9 sucrose + H2O - Lactobacillus gasseri alpha-D-glucose + beta-D-fructose - ? 374674 2.4.1.9 sucrose + H2O hydrolytic activity Limosilactobacillus reuteri alpha-D-glucose + beta-D-fructose - ? 374674 2.4.1.9 sucrose + H2O hydrolytic activity Leuconostoc citreum alpha-D-glucose + beta-D-fructose - ? 374674 2.4.1.9 sucrose + H2O hydrolytic activity, ratio of transglycosylation to hydrolysis activities is almost 1:1 at 37°C but increases about 26fold at 55°C Lactobacillus johnsonii alpha-D-glucose + beta-D-fructose - ? 374674 2.4.1.9 sucrose + H2O hydrolytic activity. Transglycosylation activity is higher in the whole concentration range tested and completely dominating at higher sucrose concentrations Aureobasidium pullulans alpha-D-glucose + beta-D-fructose - ? 374674 2.4.1.9 sucrose + H2O - Leuconostoc citreum CW2 alpha-D-glucose + beta-D-fructose - ? 374674 2.4.1.9 sucrose + H2O hydrolytic activity, ratio of transglycosylation to hydrolysis activities is almost 1:1 at 37°C but increases about 26fold at 55°C Lactobacillus johnsonii NCC 533 alpha-D-glucose + beta-D-fructose - ? 374674 2.4.1.9 sucrose + H2O hydrolytic activity Leuconostoc citreum CW28 alpha-D-glucose + beta-D-fructose - ? 374674 2.4.1.9 sucrose + H2O hydrolytic activity Limosilactobacillus reuteri 121 alpha-D-glucose + beta-D-fructose - ? 374674 2.4.1.9 sucrose + H2O - Lactobacillus gasseri DSM 20604 alpha-D-glucose + beta-D-fructose - ? 374674 2.4.1.9 sucrose + hydroquinone - Leuconostoc citreum ? - ? 421555 2.4.1.9 sucrose + kestose - Limosilactobacillus reuteri ? - ? 389816 2.4.1.9 sucrose + kestose - Limosilactobacillus reuteri 121 ? - ? 389816 2.4.1.9 sucrose + melibiose - Lactobacillus gasseri glucose + ? - ? 435859 2.4.1.9 sucrose + melibiose - Lactobacillus gasseri DSM 20604 glucose + ? - ? 435859 2.4.1.9 sucrose + raffinose synthesizes fructosylraffinose (most likely GalGF2) and a range of larger oligomers (up to GalGF6), and some polymeric material Limosilactobacillus reuteri ? - ? 389830 2.4.1.9 sucrose + raffinose synthesizes fructosylraffinose (most likely GalGF2) and a range of larger oligomers (up to GalGF6), and some polymeric material Limosilactobacillus reuteri 121 ? - ? 389830 2.4.1.9 sucrose + raffinose - Lactobacillus gasseri glucose + 1-kestose i.e. beta-D-fructofuranosyl-(2->1)-beta-D-fructofuranosyl-(2->1)-alpha-D-glucopyranoside ? 435861 2.4.1.9 sucrose + raffinose - Lactobacillus gasseri DSM 20604 glucose + 1-kestose i.e. beta-D-fructofuranosyl-(2->1)-beta-D-fructofuranosyl-(2->1)-alpha-D-glucopyranoside ? 435861 2.4.1.9 sucrose + sucrose - Paenibacillus macerans [(beta-D-Fruf-(2-1))5]-alpha-D-Glup + alpha-D-glucose the enzyme selectively produces GF5 from sucose ? 323046 2.4.1.9 sucrose + sucrose - Paenibacillus macerans [(beta-D-Fruf-(2-1))5]-alpha-D-Glup + alpha-D-glucose the enzyme selectively produces GF4 from sucose ? 323046 2.4.1.9 sucrose + sucrose - Paenibacillus macerans EG-6 [(beta-D-Fruf-(2-1))5]-alpha-D-Glup + alpha-D-glucose the enzyme selectively produces GF4 from sucose ? 323046 2.4.1.9 sucrose + sucrose - Paenibacillus macerans EG-7 [(beta-D-Fruf-(2-1))5]-alpha-D-Glup + alpha-D-glucose the enzyme selectively produces GF5 from sucose ? 323046 2.4.1.9 sucrose + sucrose at sucrose concentrations lower than 200 mM (at 37°C), hydrolysis is the main enzyme activity. At higher sucrose concentrations, transglycosylation increases gradually, reaching 90% or more of total enzyme activity at 1.7 M sucrose. From the very early stage of the reaction, after 5 min incubation, traces of nystose are visible. The nystose concentration remains constant once it reached a similar level to kestose (after 1 h), and synthesis of FOS of a larger size starts. The enzyme synthesizes mainly a broad range of fructooligosaccharides of the inulin type in a non-processive reaction Limosilactobacillus reuteri kestose + nystose + fructooligosaccharides - ? 389833 2.4.1.9 sucrose + sucrose at sucrose concentrations lower than 200 mM (at 37°C), hydrolysis is the main enzyme activity. At higher sucrose concentrations, transglycosylation increases gradually, reaching 90% or more of total enzyme activity at 1.7 M sucrose. From the very early stage of the reaction, after 5 min incubation, traces of nystose are visible. The nystose concentration remains constant once it reached a similar level to kestose (after 1 h), and synthesis of FOS of a larger size starts. The enzyme synthesizes mainly a broad range of fructooligosaccharides of the inulin type in a non-processive reaction Limosilactobacillus reuteri 121 kestose + nystose + fructooligosaccharides - ? 389833 2.4.1.9 sucrose + sucrose transglycosylation activity, ratio of transglycosylation to hydrolysis activities is almost 1:1 at 37°C but increases about 26fold at 55°C Lactobacillus johnsonii [(beta-D-fructose-(2-1))n]-alpha-D-glucose + alpha-D-glucose - ? 398381 2.4.1.9 sucrose + sucrose transgylcosylation activity. Transglycosylation activity is higher in the whole concentration range tested and completely dominating at higher sucrose concentrations Aureobasidium pullulans [(beta-D-fructose-(2-1))n]-alpha-D-glucose + alpha-D-glucose - ? 398381 2.4.1.9 sucrose + sucrose transglycosylation activity, ratio of transglycosylation to hydrolysis activities is almost 1:1 at 37°C but increases about 26fold at 55°C Lactobacillus johnsonii NCC 533 [(beta-D-fructose-(2-1))n]-alpha-D-glucose + alpha-D-glucose - ? 398381 2.4.1.9 sucrose + sucrose - Limosilactobacillus reuteri 1-kestose + nystose + fructooligosaccharides - ? 456792 2.4.1.9 sucrose + sucrose - Limosilactobacillus reuteri 121 1-kestose + nystose + fructooligosaccharides - ? 456792 2.4.1.9 sucrose + sucrose - Limosilactobacillus reuteri alpha-D-glucose + inulin nanoparticles - ? 456794 2.4.1.9 sucrose + sucrose - Limosilactobacillus reuteri 121 alpha-D-glucose + inulin nanoparticles - ? 456794 2.4.1.9 sucrose + sucrose - Streptococcus mutans alpha-D-glucose + inulin-type fructan - ? 456795 2.4.1.9 sucrose + sucrose - Limosilactobacillus reuteri alpha-D-glucose + inulin-type fructan - ? 456795 2.4.1.9 sucrose + sucrose - Lactobacillus gasseri alpha-D-glucose + inulin-type fructan - ? 456795 2.4.1.9 sucrose + sucrose - Leuconostoc citreum alpha-D-glucose + inulin-type fructan - ? 456795 2.4.1.9 sucrose + sucrose - Lactobacillus johnsonii alpha-D-glucose + inulin-type fructan - ? 456795 2.4.1.9 sucrose + sucrose - Salipaludibacillus agaradhaerens alpha-D-glucose + inulin-type fructan - ? 456795 2.4.1.9 sucrose + sucrose - Weissella confusa alpha-D-glucose + inulin-type fructan - ? 456795 2.4.1.9 sucrose + sucrose - Streptomyces viridochromogenes alpha-D-glucose + inulin-type fructan - ? 456795 2.4.1.9 sucrose + sucrose - Aspergillus sydowii alpha-D-glucose + inulin-type fructan - ? 456795 2.4.1.9 sucrose + sucrose - Bacillus sp. 217C-11 alpha-D-glucose + inulin-type fructan - ? 456795 2.4.1.9 sucrose + sucrose - Weissella confusa MBFCNC-2(1) alpha-D-glucose + inulin-type fructan - ? 456795 2.4.1.9 sucrose + sucrose - Streptococcus mutans JC-1 alpha-D-glucose + inulin-type fructan - ? 456795 2.4.1.9 sucrose + sucrose - Streptomyces viridochromogenes DSM 40736 alpha-D-glucose + inulin-type fructan - ? 456795 2.4.1.9 sucrose + sucrose - Streptococcus mutans JC-2 alpha-D-glucose + inulin-type fructan - ? 456795 2.4.1.9 sucrose + sucrose - Salipaludibacillus agaradhaerens WDG185 alpha-D-glucose + inulin-type fructan - ? 456795 2.4.1.9 sucrose + sucrose - Lactobacillus johnsonii NCC 533 alpha-D-glucose + inulin-type fructan - ? 456795 2.4.1.9 sucrose + sucrose - Leuconostoc citreum CW28 alpha-D-glucose + inulin-type fructan - ? 456795 2.4.1.9 sucrose + sucrose - Aspergillus sydowii IAM 2544 alpha-D-glucose + inulin-type fructan - ? 456795 2.4.1.9 sucrose + sucrose - Limosilactobacillus reuteri 121 alpha-D-glucose + inulin-type fructan - ? 456795 2.4.1.9 sucrose + sucrose - Limosilactobacillus reuteri TMW1.106 alpha-D-glucose + inulin-type fructan - ? 456795 2.4.1.9 sucrose + sucrose - Lactobacillus gasseri DSM 20604 alpha-D-glucose + inulin-type fructan - ? 456795 2.4.1.9 sucrose + sucrose - Lactobacillus gasseri DSM 20243 alpha-D-glucose + inulin-type fructan - ? 456795 2.4.1.9 sucrose + sucrose - Streptococcus mutans BHT alpha-D-glucose + inulin-type fructan - ? 456795 2.4.1.9 sucrose + sucrose - Streptomyces viridochromogenes alpha-D-glucose + inulin-type fructan polymer - ? 456796 2.4.1.9 sucrose + sucrose - Streptomyces viridochromogenes DSM 40736 alpha-D-glucose + inulin-type fructan polymer - ? 456796 2.4.1.9 sucrose + sucrose - Limosilactobacillus reuteri alpha-D-glucose + inulin-type fructooligosaccharides - ? 456797 2.4.1.9 sucrose + sucrose - Limosilactobacillus reuteri 121 alpha-D-glucose + inulin-type fructooligosaccharides - ? 456797 2.4.1.9 sucrose + sucrose - Limosilactobacillus reuteri alpha-D-glucose + inulin-type oligosaccharide + inulin - ? 456798 2.4.1.9 sucrose + sucrose - Leuconostoc citreum alpha-D-glucose + inulin-type oligosaccharide + inulin - ? 456798 2.4.1.9 sucrose + sucrose - Lactobacillus johnsonii alpha-D-glucose + inulin-type oligosaccharide + inulin - ? 456798 2.4.1.9 sucrose + sucrose - Lactobacillus gasseri alpha-D-glucose + inulin-type oligosaccharide + inulin - ? 456798 2.4.1.9 sucrose + sucrose - Lactobacillus johnsonii NCC 533 alpha-D-glucose + inulin-type oligosaccharide + inulin - ? 456798 2.4.1.9 sucrose + sucrose - Leuconostoc citreum CW28 alpha-D-glucose + inulin-type oligosaccharide + inulin - ? 456798 2.4.1.9 sucrose + sucrose - Limosilactobacillus reuteri 121 alpha-D-glucose + inulin-type oligosaccharide + inulin - ? 456798 2.4.1.9 sucrose + sucrose - Limosilactobacillus reuteri TMW1.106 alpha-D-glucose + inulin-type oligosaccharide + inulin - ? 456798 2.4.1.9 sucrose + sucrose - Lactobacillus gasseri DSM 20604 alpha-D-glucose + inulin-type oligosaccharide + inulin - ? 456798 2.4.1.9 sucrose + sucrose with 300 g/l sucrose, 128.4 g/l of fructooligosaccharide is produced with 85.6% conversion yield. From 400 g/l sucrose, 152.6 g/l of fructooligosaccharides is produced with 76.3% conversion yield Limosilactobacillus reuteri alpha-D-glucose + medium chain fructooligosaccharides (DP 2-20) - ? 456799 2.4.1.9 sucrose + [(2->1)-beta-D-fructosyl]n - Lactobacillus gasseri glucose + [(2->1)-beta-D-fructosyl]n+1 - ? 419610 2.4.1.9 sucrose + [(2->1)-beta-D-fructosyl]n - Lactobacillus johnsonii glucose + [(2->1)-beta-D-fructosyl]n+1 - ? 419610 2.4.1.9 sucrose + [(2->1)-beta-D-fructosyl]n - Leuconostoc citreum glucose + [(2->1)-beta-D-fructosyl]n+1 - ? 419610 2.4.1.9 sucrose + [(2->1)-beta-D-fructosyl]n - Limosilactobacillus reuteri glucose + [(2->1)-beta-D-fructosyl]n+1 - ? 419610 2.4.1.9 sucrose + [(2->1)-beta-D-fructosyl]n via first transfructosylation product 1-kestose Limosilactobacillus reuteri glucose + [(2->1)-beta-D-fructosyl]n+1 - ? 419610 2.4.1.9 sucrose + [(2->1)-beta-D-fructosyl]n via first transfructosylation product 1-kestose, binding mode, overview, an inulin-type linkage is formed. Particular residues from the nonconserved 1B-1C loop determine product linkage type specificity in GH68 fructansucrases Lactobacillus johnsonii glucose + [(2->1)-beta-D-fructosyl]n+1 - ? 419610 2.4.1.9 sucrose + [(2->1)-beta-D-fructosyl]n - Lactobacillus johnsonii NCC 533 glucose + [(2->1)-beta-D-fructosyl]n+1 - ? 419610 2.4.1.9 sucrose + [(2->1)-beta-D-fructosyl]n via first transfructosylation product 1-kestose, binding mode, overview, an inulin-type linkage is formed. Particular residues from the nonconserved 1B-1C loop determine product linkage type specificity in GH68 fructansucrases Lactobacillus johnsonii NCC 533 glucose + [(2->1)-beta-D-fructosyl]n+1 - ? 419610 2.4.1.9 sucrose + [(2->1)-beta-D-fructosyl]n - Limosilactobacillus reuteri 121 glucose + [(2->1)-beta-D-fructosyl]n+1 - ? 419610 2.4.1.9 sucrose + [(2->1)-beta-D-fructosyl]n via first transfructosylation product 1-kestose Limosilactobacillus reuteri 121 glucose + [(2->1)-beta-D-fructosyl]n+1 - ? 419610 2.4.1.9 sucrose + [(2->1)-beta-D-fructosyl]n - Lactobacillus gasseri DSM 20604 glucose + [(2->1)-beta-D-fructosyl]n+1 - ? 419610 2.4.1.9 sucrose + [(2->1)-beta-D-fructosyl]n - Streptococcus mutans alpha-D-glucose + [(2->1)-beta-D-fructosyl]n+1 - ? 454655 2.4.1.9 sucrose + [(2->1)-beta-D-fructosyl]n - Limosilactobacillus reuteri alpha-D-glucose + [(2->1)-beta-D-fructosyl]n+1 - ? 454655 2.4.1.9 sucrose + [(2->1)-beta-D-fructosyl]n - Streptomyces viridochromogenes alpha-D-glucose + [(2->1)-beta-D-fructosyl]n+1 - ? 454655 2.4.1.9 sucrose + [(2->1)-beta-D-fructosyl]n - Lactobacillus gasseri alpha-D-glucose + [(2->1)-beta-D-fructosyl]n+1 - ? 454655 2.4.1.9 sucrose + [(2->1)-beta-D-fructosyl]n - Leuconostoc citreum alpha-D-glucose + [(2->1)-beta-D-fructosyl]n+1 - ? 454655 2.4.1.9 sucrose + [(2->1)-beta-D-fructosyl]n - Lactobacillus johnsonii alpha-D-glucose + [(2->1)-beta-D-fructosyl]n+1 - ? 454655 2.4.1.9 sucrose + [(2->1)-beta-D-fructosyl]n - Salipaludibacillus agaradhaerens alpha-D-glucose + [(2->1)-beta-D-fructosyl]n+1 - ? 454655 2.4.1.9 sucrose + [(2->1)-beta-D-fructosyl]n - Weissella confusa alpha-D-glucose + [(2->1)-beta-D-fructosyl]n+1 - ? 454655 2.4.1.9 sucrose + [(2->1)-beta-D-fructosyl]n - Aspergillus sydowii alpha-D-glucose + [(2->1)-beta-D-fructosyl]n+1 - ? 454655 2.4.1.9 sucrose + [(2->1)-beta-D-fructosyl]n - Bacillus sp. 217C-11 alpha-D-glucose + [(2->1)-beta-D-fructosyl]n+1 - ? 454655 2.4.1.9 sucrose + [(2->1)-beta-D-fructosyl]n - Weissella confusa MBFCNC-2(1) alpha-D-glucose + [(2->1)-beta-D-fructosyl]n+1 - ? 454655 2.4.1.9 sucrose + [(2->1)-beta-D-fructosyl]n - Streptococcus mutans JC-1 alpha-D-glucose + [(2->1)-beta-D-fructosyl]n+1 - ? 454655 2.4.1.9 sucrose + [(2->1)-beta-D-fructosyl]n - Streptomyces viridochromogenes DSM 40736 alpha-D-glucose + [(2->1)-beta-D-fructosyl]n+1 - ? 454655 2.4.1.9 sucrose + [(2->1)-beta-D-fructosyl]n - Streptococcus mutans JC-2 alpha-D-glucose + [(2->1)-beta-D-fructosyl]n+1 - ? 454655 2.4.1.9 sucrose + [(2->1)-beta-D-fructosyl]n - Salipaludibacillus agaradhaerens WDG185 alpha-D-glucose + [(2->1)-beta-D-fructosyl]n+1 - ? 454655 2.4.1.9 sucrose + [(2->1)-beta-D-fructosyl]n - Lactobacillus johnsonii NCC 533 alpha-D-glucose + [(2->1)-beta-D-fructosyl]n+1 - ? 454655 2.4.1.9 sucrose + [(2->1)-beta-D-fructosyl]n - Leuconostoc citreum CW28 alpha-D-glucose + [(2->1)-beta-D-fructosyl]n+1 - ? 454655 2.4.1.9 sucrose + [(2->1)-beta-D-fructosyl]n - Aspergillus sydowii IAM 2544 alpha-D-glucose + [(2->1)-beta-D-fructosyl]n+1 - ? 454655 2.4.1.9 sucrose + [(2->1)-beta-D-fructosyl]n - Limosilactobacillus reuteri 121 alpha-D-glucose + [(2->1)-beta-D-fructosyl]n+1 - ? 454655 2.4.1.9 sucrose + [(2->1)-beta-D-fructosyl]n - Limosilactobacillus reuteri TMW1.106 alpha-D-glucose + [(2->1)-beta-D-fructosyl]n+1 - ? 454655 2.4.1.9 sucrose + [(2->1)-beta-D-fructosyl]n - Lactobacillus gasseri DSM 20604 alpha-D-glucose + [(2->1)-beta-D-fructosyl]n+1 - ? 454655 2.4.1.9 sucrose + [(2->1)-beta-D-fructosyl]n - Lactobacillus gasseri DSM 20243 alpha-D-glucose + [(2->1)-beta-D-fructosyl]n+1 - ? 454655 2.4.1.9 sucrose + [(2->1)-beta-D-fructosyl]n - Streptococcus mutans BHT alpha-D-glucose + [(2->1)-beta-D-fructosyl]n+1 - ? 454655 2.4.1.9 sucrose + [(beta-D-Fruf-(2-1))n]-alpha-D-Glup - Paenibacillus macerans alpha-D-glucose + [(beta-D-Fruf-(2-1))n+1]-alpha-D-Glup - ? 323045 2.4.1.9 sucrose + [(beta-D-Fruf-(2-1))n]-alpha-D-Glup - Helianthus tuberosus alpha-D-glucose + [(beta-D-Fruf-(2-1))n+1]-alpha-D-Glup - ? 323045 2.4.1.9 sucrose + [(beta-D-Fruf-(2-1))n]-alpha-D-Glup - Limosilactobacillus reuteri alpha-D-glucose + [(beta-D-Fruf-(2-1))n+1]-alpha-D-Glup - ? 323045 2.4.1.9 sucrose + [(beta-D-Fruf-(2-1))n]-alpha-D-Glup - Leuconostoc citreum alpha-D-glucose + [(beta-D-Fruf-(2-1))n+1]-alpha-D-Glup - ? 323045 2.4.1.9 sucrose + [(beta-D-Fruf-(2-1))n]-alpha-D-Glup the recombinant truncated enzyme produces both the fructooligosaccharides 1-kestose and nystose and high-molecular-weight-fructan with beta-(2, 1) linkages. The wild-type enzyme only produces 1-kestose, 95%, and nystose, 5% Limosilactobacillus reuteri alpha-D-glucose + [(beta-D-Fruf-(2-1))n+1]-alpha-D-Glup - ? 323045 2.4.1.9 sucrose + [(beta-D-Fruf-(2-1))n]-alpha-D-Glup wild-type and recombinant enzyme produce inulin from sucose Leuconostoc citreum alpha-D-glucose + [(beta-D-Fruf-(2-1))n+1]-alpha-D-Glup - ? 323045 2.4.1.9 sucrose + [(beta-D-Fruf-(2-1))n]-alpha-D-Glup the enzyme selectively produces GF5 from sucose Paenibacillus macerans alpha-D-glucose + [(beta-D-Fruf-(2-1))n+1]-alpha-D-Glup - ? 323045 2.4.1.9 sucrose + [(beta-D-Fruf-(2-1))n]-alpha-D-Glup fructoologosaccharides are synthesized from sucrose at the expense of fructose residues from inulin and related polysaccharides. Fructose residues are transferred from polysaccharide to sucrose molecules, forming a trisaccharide in the first instance. Further fructose residues might then be transferred to the trisaccharide, yielding a tetrasaccharide, and so on. No significant reaction with sucrose alone Helianthus tuberosus alpha-D-glucose + [(beta-D-Fruf-(2-1))n+1]-alpha-D-Glup - ? 323045 2.4.1.9 sucrose + [(beta-D-Fruf-(2-1))n]-alpha-D-Glup - Paenibacillus macerans EG-6 alpha-D-glucose + [(beta-D-Fruf-(2-1))n+1]-alpha-D-Glup - ? 323045 2.4.1.9 sucrose + [(beta-D-Fruf-(2-1))n]-alpha-D-Glup the enzyme selectively produces GF5 from sucose Paenibacillus macerans EG-6 alpha-D-glucose + [(beta-D-Fruf-(2-1))n+1]-alpha-D-Glup - ? 323045 2.4.1.9 sucrose + [(beta-D-Fruf-(2-1))n]-alpha-D-Glup - Paenibacillus macerans EG-7 alpha-D-glucose + [(beta-D-Fruf-(2-1))n+1]-alpha-D-Glup - ? 323045 2.4.1.9 sucrose + [(beta-D-Fruf-(2-1))n]-alpha-D-Glup the enzyme selectively produces GF5 from sucose Paenibacillus macerans EG-7 alpha-D-glucose + [(beta-D-Fruf-(2-1))n+1]-alpha-D-Glup - ? 323045