2.1.1.241	additional information	the only substance that serves as a substrate for the recombinant Bx7 is TRIBOA-Glc, neither the aglucone TRIBOA nor well-known substrates of plant O-methyltransferases are efficiently converted by the enzyme. In case of quercetin, a minor activity is detected. BX7 has no catalytic activity toward phenylpropanoids and flavonoids, e.g. apigenin is no substrate	89	
2.1.1.241	S-adenosyl-L-methionine + (2R)-4,7-dihydroxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside	-	422036	
2.1.1.241	S-adenosyl-L-methionine + (2R)-4,7-dihydroxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside	conversion of (2R)-2,4,7-trihydroxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside, TRIBOA-Glc, to DIMBOA-Glc	422036	
2.1.1.241	S-adenosyl-L-methionine + 2-(2,4,7-trihydroxy-8-methoxy-1,4-benzoxazin-3-one)-beta-D-glucopyranose	i.e. TRIMBOA-Glc	454484	
2.1.1.241	S-adenosyl-L-methionine + 2-(2,4,7-trihydroxy-8-methoxy-1,4-benzoxazin-3-one)-beta-D-glucopyranose	i.e. TRIMBOA-Glc, enzyme BX7 methylates the C-7 hydroxyl group of TRIBOA-Glc. Recombinant BX7 also accepts TRIMBOA-Glc as substrate and methylates the hydroxyl group at C-7	454484