1.8.1.15 2 mycothiol + NADP+ - Corynebacterium glutamicum mycothione + NADPH + H+ - r 461450 1.8.1.15 2,6-dimethylbenzoquinone + NADPH - Mycobacterium tuberculosis ? - ? 189474 1.8.1.15 2,6-dimethylbenzoquinone + NADPH - Mycobacterium tuberculosis H37Rv ? - ? 189474 1.8.1.15 2-(-N-acetyl-L-cysteinyl) amino-2-deoxy-alpha-D-glucopyranoside disulfide + NADPH - Mycobacterium tuberculosis ? - ? 395407 1.8.1.15 2-methyl-1,4-naphthoquinone + alpha-NADPH - Mycobacterium tuberculosis ? - ? 189478 1.8.1.15 5,8-dihydroxy-1,4-naphthoquinone + NADPH - Mycobacterium tuberculosis ? - ? 189477 1.8.1.15 5-(benzyl 2-(-N-acetyl-L-cysteinyl) amino-2-deoxy-alpha-D-glucopyranoside)-dithio-2-nitrobenzoate + NADPH a 5,5'-dithiobis-2-nitrobenzoic acid (DTNB)-coupled assay is developed. The mixed disulfide substrate liberates one molecule of TNB on NADPH-dependent reduction by Mycobacterium tubercolosis reductase. The liberated mycothiol analogue then reacts with DTNB to regenerate the mixed disulfide substrate and another molecule of TNB. Reaction progress can be measured by the increase in absorbance (412 nm) from the two molecules of TNB produced per turn of this catalytic cycle Mycobacterium tuberculosis ? - ? 408200 1.8.1.15 5-hydroxy-1,4-naphthoquinone + NADPH - Mycobacterium tuberculosis ? - ? 189476 1.8.1.15 5-hydroxy-1,4-naphthoquinone + NADPH - Mycobacterium tuberculosis H37Rv ? - ? 189476 1.8.1.15 7-methyljuglone + NADPH potent subversive substrate Mycobacterium tuberculosis ? - ? 396103 1.8.1.15 8-chloro-5-methoxy-7-methyl-1,4-naphthoquinone + NADPH - Mycobacterium tuberculosis ? - ? 396112 1.8.1.15 AcCys-GlcN + NADPH - Mycobacterium tuberculosis ? - ? 396185 1.8.1.15 AcCys-GlcN-1-O-CH2Ph + NADPH - Mycobacterium tuberculosis ? - ? 396186 1.8.1.15 AcCys-GlcN-1-O-CH3 + NADPH - Mycobacterium tuberculosis ? - ? 396187 1.8.1.15 benzyl 2-(-N-acetyl-L-cysteinyl) amino-2-deoxy-alpha-D-glucopyranoside disulfide + NADPH - Mycobacterium tuberculosis ? - ? 396579 1.8.1.15 benzyl 2-(N-acetyl-L-cysteinyl)amino-2-deoxy-alpha-D-glucopyranoside disulfide + NADPH - Mycobacterium tuberculosis ? - ? 408768 1.8.1.15 des-myo-inositol mycothione + NADH - Mycobacterium tuberculosis ? - ? 189480 1.8.1.15 des-myo-inositol mycothione + reduced beta-nicotinamide hypoxanthine dinucleotide - Mycobacterium tuberculosis ? - ? 189481 1.8.1.15 des-myo-inositol mycothione + reduced beta-nicotinamide hypoxanthine dinucleotide phosphate - Mycobacterium tuberculosis ? - ? 189479 1.8.1.15 des-myo-inositol mycothione + thio-NADH - Mycobacterium tuberculosis ? - ? 189483 1.8.1.15 des-myo-inositol mycothione + thio-NADPH - Mycobacterium tuberculosis ? - ? 189482 1.8.1.15 desmyoinositol mycothione + NADPH - Mycobacterium tuberculosis ? - ? 189473 1.8.1.15 desmyoinositol mycothione + NADPH - Mycobacterium tuberculosis H37Rv ? - ? 189473 1.8.1.15 dimeric mycothiol disulfide + NADPH - Mycobacterium tuberculosis 2 mycothione + H+ - ? 409295 1.8.1.15 diospyrin + NADPH subversive substrate Mycobacterium tuberculosis ? - ? 397074 1.8.1.15 DTNB + NADPH - Mycobacterium tuberculosis ? - ? 189475 1.8.1.15 DTNB + NADPH - Mycobacterium tuberculosis H37Rv ? - ? 189475 1.8.1.15 juglone + NADPH subversive substrate Mycobacterium tuberculosis ? - ? 397493 1.8.1.15 menadione + NADPH subversive substrate Mycobacterium tuberculosis ? - ? 187323 1.8.1.15 methyl 2-(-N-acetyl-L-cysteinyl) amino-2-deoxy-alpha-D-glucopyranoside disulfide + NADPH - Mycobacterium tuberculosis ? - ? 397757 1.8.1.15 additional information many naphthoquinones operate as subversive substrates. The native functions of these enzyme involves the NADPH-dependent reduction of disulfide bonds in the substrate. The enzyme-mediated toxicity of quinones/naphthoquinones is a consequence of their enzymatic reduction to semiquinone radicals. The naphthoquinone is then regenerated via the concomitant reduction of oxygen to toxic superoxide anion radicals. In this manner the naphthoquinone substrate is regenerated and the futile redox cycle continues Mycobacterium tuberculosis ? - ? 89 1.8.1.15 additional information mycothiol disulfide reductase (Mtr) interacts with the oxido-reductase Mycoredoxin-1 (Mrx-1). the MtMtr-MtMrx-1 interaction is characterized by a fast exchange regime, critical residues by NMR spectroscopy and docking studies, overview Mycobacterium tuberculosis ? - ? 89 1.8.1.15 additional information mycothiol disulfide reductase (Mtr) interacts with the oxido-reductase Mycoredoxin-1 (Mrx-1). the MtMtr-MtMrx-1 interaction is characterized by a fast exchange regime, critical residues by NMR spectroscopy and docking studies, overview Mycobacterium tuberculosis H37Rv ? - ? 89 1.8.1.15 mycothione + NADPH - Mycobacterium tuberculosis mycothiol + NADP+ - ? 189472 1.8.1.15 mycothione + NADPH the enzyme is involved in mycothiol metabolism. Mycothiol may play an important role in the survival and adaption of mycobacteria to oxidative stress caused by normal metabolism, ir induced by the action of anti-tubercular drugs Mycobacterium tuberculosis variant bovis mycothiol + NADP+ - ? 189472 1.8.1.15 mycothione + NADPH - Mycobacterium tuberculosis H37Rv mycothiol + NADP+ - ? 189472 1.8.1.15 mycothione + NADPH + H+ - Corynebacterium glutamicum mycothiol + NADP+ - ? 409902 1.8.1.15 mycothione + NADPH + H+ - Corynebacterium glutamicum 2 mycothiol + NADP+ - ? 440330 1.8.1.15 mycothione + NADPH + H+ - Corynebacterium glutamicum 2 mycothiol + NADP+ - r 440330 1.8.1.15 mycothione + NADPH + H+ - Mycobacterium tuberculosis 2 mycothiol + NADP+ - ? 440330 1.8.1.15 mycothione + NADPH + H+ - Rhodococcus erythropolis 2 mycothiol + NADP+ - ? 440330 1.8.1.15 mycothione + NADPH + H+ - Mycobacterium tuberculosis H37Rv 2 mycothiol + NADP+ - ? 440330 1.8.1.15 mycothione + NADPH + H+ - Rhodococcus erythropolis PR4 2 mycothiol + NADP+ - ? 440330 1.8.1.15 neodiospyrin + NADPH subversive substrate Mycobacterium tuberculosis ? - ? 397972 1.8.1.15 SeO32- + NADPH + H+ the enzyme exibits a strong substrate preference for TeO32- over SeO32- Rhodococcus erythropolis ? - ? 463023 1.8.1.15 SeO32- + NADPH + H+ the enzyme exibits a strong substrate preference for TeO32- over SeO32- Rhodococcus erythropolis PR4 ? - ? 463023 1.8.1.15 TeO32- + NADPH + H+ the enzyme exibits a strong substrate preference for TeO32- over SeO32- Rhodococcus erythropolis ? - ? 463051 1.8.1.15 TeO32- + NADPH + H+ the enzyme exibits a strong substrate preference for TeO32- over SeO32- Rhodococcus erythropolis PR4 ? - ? 463051