1.2.1.89	D-glyceraldehyde + NAD+	NAD+ is a very poor cofactor for wild-type	Thermoplasma acidophilum	D-glycerate + NADH + H+	-	?	435434	
1.2.1.89	D-glyceraldehyde + NAD+ + H2O	activity of mutants N286E, N286H, N286T. No substrate for wild-type	Escherichia coli	D-glycerate + NADH + H+	-	?	423256	
1.2.1.89	D-glyceraldehyde + NADP+ + H2O	-	Thermoplasma acidophilum	D-glycerate + NADPH + H+	-	?	424757	
1.2.1.89	D-glyceraldehyde + NADP+ + H2O	the enzyme is involved in downstream portion of the non-phosphorylated Entner–Doudoroff pathway	Thermoplasma acidophilum	D-glycerate + NADPH + H+	-	?	424757	
1.2.1.89	D-glyceraldehyde + NADP+ + H2O	the enzyme is involved in the non-phosphorylative Entner–Doudoroff pathway	Thermoplasma acidophilum	D-glycerate + NADPH + H+	-	?	424757	
1.2.1.89	D-glyceraldehyde + NADP+ + H2O	the enzyme is involved in the non-phosphorylative Entner–Doudoroff pathway	Picrophilus torridus	D-glycerate + NADPH + H+	-	?	424757	
1.2.1.89	D-glyceraldehyde + NADP+ + H2O	also accepts NAD+ under technically relevant conditions, cofactor above 1 mM	Thermoplasma acidophilum	D-glycerate + NADPH + H+	-	?	424757	
1.2.1.89	D-glyceraldehyde + NADP+ + H2O	completely inactive when NAD+ is substituted for NADP+	Thermoplasma acidophilum	D-glycerate + NADPH + H+	-	?	424757	
1.2.1.89	D-glyceraldehyde + NADP+ + H2O	completely inactive with NAD+	Thermoplasma acidophilum	D-glycerate + NADPH + H+	-	?	424757	
1.2.1.89	D-glyceraldehyde + NADP+ + H2O	completely inactive with NAD+	Picrophilus torridus	D-glycerate + NADPH + H+	-	?	424757	
1.2.1.89	D-glyceraldehyde + NADP+ + H2O	the enzyme is involved in downstream portion of the non-phosphorylated Entner–Doudoroff pathway	Thermoplasma acidophilum JCM 9062	D-glycerate + NADPH + H+	-	?	424757	
1.2.1.89	D-glyceraldehyde + NADP+ + H2O	completely inactive when NAD+ is substituted for NADP+	Thermoplasma acidophilum JCM 9062	D-glycerate + NADPH + H+	-	?	424757	
1.2.1.89	D-glyceraldehyde + NADP+ + H2O	the enzyme is involved in the non-phosphorylative Entner–Doudoroff pathway	Thermoplasma acidophilum JCM 9062	D-glycerate + NADPH + H+	-	?	424757	
1.2.1.89	D-glyceraldehyde + NADP+ + H2O	completely inactive with NAD+	Thermoplasma acidophilum JCM 9062	D-glycerate + NADPH + H+	-	?	424757	
1.2.1.89	D-glyceraldehyde + NADP+ + H2O	also accepts NAD+ under technically relevant conditions, cofactor above 1 mM	Thermoplasma acidophilum JCM 9062	D-glycerate + NADPH + H+	-	?	424757	
1.2.1.89	D-glyceraldehyde + NADP+ + H2O	the enzyme is involved in the non-phosphorylative Entner–Doudoroff pathway	Picrophilus torridus DSM 9790	D-glycerate + NADPH + H+	-	?	424757	
1.2.1.89	D-glyceraldehyde + NADP+ + H2O	completely inactive with NAD+	Picrophilus torridus DSM 9790	D-glycerate + NADPH + H+	-	?	424757	
1.2.1.89	D-glyceraldehyde + NADP+ + H2O	-	Thermoplasma acidophilum ATCC 25905	D-glycerate + NADPH + H+	-	?	424757	
1.2.1.89	D-glyceraldehyde 3-phosphate + NADP+ + H2O	activity is 33% compared to the activity with D-glyceraldehyde	Thermoplasma acidophilum	D-glycerate 3-phosphate + NADPH + H+	-	?	426219	
1.2.1.89	D-glyceraldehyde 3-phosphate + NADP+ + H2O	catalytic efficiency (kcat/Km) for D-glyceraldehyde 3-phosphate is 1.4% compared to the catalytic efficiency with D-glyceraldehyde	Picrophilus torridus	D-glycerate 3-phosphate + NADPH + H+	-	?	426219	
1.2.1.89	D-glyceraldehyde 3-phosphate + NADP+ + H2O	catalytic efficiency (kcat/Km) for glyceraldehyde 3-phosphate is less than 1% compared to the catalytic efficiency with D-glyceraldehyde	Thermoplasma acidophilum	D-glycerate 3-phosphate + NADPH + H+	-	?	426219	
1.2.1.89	D-glyceraldehyde 3-phosphate + NADP+ + H2O	activity is 33% compared to the activity with D-glyceraldehyde	Thermoplasma acidophilum JCM 9062	D-glycerate 3-phosphate + NADPH + H+	-	?	426219	
1.2.1.89	D-glyceraldehyde 3-phosphate + NADP+ + H2O	catalytic efficiency (kcat/Km) for glyceraldehyde 3-phosphate is less than 1% compared to the catalytic efficiency with D-glyceraldehyde	Thermoplasma acidophilum JCM 9062	D-glycerate 3-phosphate + NADPH + H+	-	?	426219	
1.2.1.89	D-glyceraldehyde 3-phosphate + NADP+ + H2O	catalytic efficiency (kcat/Km) for D-glyceraldehyde 3-phosphate is 1.4% compared to the catalytic efficiency with D-glyceraldehyde	Picrophilus torridus DSM 9790	D-glycerate 3-phosphate + NADPH + H+	-	?	426219	
1.2.1.89	glyceraldehyde + NAD+ + H2O	-	Thermoplasma acidophilum	glycerate + NADH + H+	-	?	231697	
1.2.1.89	glycolaldehyde + NADP+	activity is 14% compared to the activity with D-glyceraldehyde	Thermoplasma acidophilum	glycolate + NADPH + H+	-	?	426457	
1.2.1.89	glycolaldehyde + NADP+	catalytic efficiency (kcat/Km) for glycolaldehyde is 1% compared to the catalytic efficiency with D-glyceraldehyde	Picrophilus torridus	glycolate + NADPH + H+	-	?	426457	
1.2.1.89	glycolaldehyde + NADP+	catalytic efficiency (kcat/Km) for glycolaldehyde is about 2% compared to the catalytic efficiency with D-glyceraldehyde	Thermoplasma acidophilum	glycolate + NADPH + H+	-	?	426457	
1.2.1.89	glycolaldehyde + NADP+	activity is 14% compared to the activity with D-glyceraldehyde	Thermoplasma acidophilum JCM 9062	glycolate + NADPH + H+	-	?	426457	
1.2.1.89	glycolaldehyde + NADP+	catalytic efficiency (kcat/Km) for glycolaldehyde is about 2% compared to the catalytic efficiency with D-glyceraldehyde	Thermoplasma acidophilum JCM 9062	glycolate + NADPH + H+	-	?	426457	
1.2.1.89	glycolaldehyde + NADP+	catalytic efficiency (kcat/Km) for glycolaldehyde is 1% compared to the catalytic efficiency with D-glyceraldehyde	Picrophilus torridus DSM 9790	glycolate + NADPH + H+	-	?	426457	
1.2.1.89	additional information	no activity towards ketoaldehyde (methylglyoxal), alkyl aldehydes (formaldehyde, acetaldehyde and propionaldehyde) or aromatic aldehydes (phenylacetaldehyde and 4-nitrobenzaldehyde)	Thermoplasma acidophilum	?	-	?	89	
1.2.1.89	additional information	no activity with acetaldehyde, propionaldehyde, n-butyraldehyde, pyruvaldehyde, isobutyraldehyde or D-glucose	Thermoplasma acidophilum	?	-	?	89	
1.2.1.89	additional information	no activity with: acetaldehyde, crotonaldehyde, benzaldehyde, succinate semialdehyde, and dihydroxyacetone	Picrophilus torridus	?	-	?	89	
1.2.1.89	additional information	NAD+ is a poor substrate	Thermoplasma acidophilum	?	-	-	89	
1.2.1.89	additional information	no activity with acetaldehyde and isobutyraldehyde	Thermoplasma acidophilum	?	-	?	89	
1.2.1.89	additional information	no activity towards ketoaldehyde (methylglyoxal), alkyl aldehydes (formaldehyde, acetaldehyde and propionaldehyde) or aromatic aldehydes (phenylacetaldehyde and 4-nitrobenzaldehyde)	Thermoplasma acidophilum JCM 9062	?	-	?	89	
1.2.1.89	additional information	no activity with acetaldehyde, propionaldehyde, n-butyraldehyde, pyruvaldehyde, isobutyraldehyde or D-glucose	Thermoplasma acidophilum JCM 9062	?	-	?	89	
1.2.1.89	additional information	no activity with: acetaldehyde, crotonaldehyde, benzaldehyde, succinate semialdehyde, and dihydroxyacetone	Picrophilus torridus DSM 9790	?	-	?	89	
1.2.1.89	additional information	NAD+ is a poor substrate	Thermoplasma acidophilum ATCC 25905	?	-	-	89