1.2.1.85	2-hydroxy-5-methyl-6-oxohexa-2,4-dienoate + NAD+ + H2O	-	Pseudomonas putida	(2E,4Z)-2-hydroxy-5-methylhexa-2,4-diendioate + NADH + H+	-	?	419902	
1.2.1.85	2-hydroxymuconate-6-semialdehyde + NAD+ + H2O	-	Pseudomonas putida	(2Z,4E)-2-hydroxyhexa-2,4-dienedioate + NADH + H+	-	?	419908	
1.2.1.85	2-hydroxymuconate-6-semialdehyde + NAD+ + H2O	100% activity	Bordetella sp.	(2Z,4E)-2-hydroxyhexa-2,4-dienedioate + NADH + H+	-	?	419908	
1.2.1.85	2-hydroxymuconate-6-semialdehyde + NAD+ + H2O	the activity of crude enzyme for 2-hydroxymuconate-6-semialdehyde in the presence of NAD+ is about 11 times higher than that achieved with NADP+	Paenibacillus sp.	(2Z,4E)-2-hydroxyhexa-2,4-dienedioate + NADH + H+	-	?	419908	
1.2.1.85	2-hydroxymuconate-6-semialdehyde + NAD+ + H2O	-	Pseudomonas veronii	(2Z,4E)-2-hydroxyhexa-2,4-dienedioate + NADH + 2 H+	-	?	440095	
1.2.1.85	2-hydroxymuconate-6-semialdehyde + NAD+ + H2O	-	Pseudomonas putida	(2Z,4E)-2-hydroxyhexa-2,4-dienedioate + NADH + 2 H+	-	?	440095	
1.2.1.85	2-hydroxymuconate-6-semialdehyde + NAD+ + H2O	-	Pseudomonas veronii 1YB2	(2Z,4E)-2-hydroxyhexa-2,4-dienedioate + NADH + 2 H+	-	?	440095	
1.2.1.85	2-hydroxymuconate-6-semialdehyde + NAD+ + H2O	-	Pseudomonas putida G7	(2Z,4E)-2-hydroxyhexa-2,4-dienedioate + NADH + 2 H+	-	?	440095	
1.2.1.85	2-hydroxymuconate-6-semialdehyde + NAD+ + H2O	-	Pseudomonas veronii 1YdBTEX2	(2Z,4E)-2-hydroxyhexa-2,4-dienedioate + NADH + 2 H+	-	?	440095	
1.2.1.85	3-chlorobenzaldehyde + NAD+ + H2O	-	Pseudomonas putida	3-chlorobenzoate + NADH + H+	-	?	419969	
1.2.1.85	3-fluorobenzaldehyde + NAD+ + H2O	-	Pseudomonas putida	3-fluorobenzoate + NADH + H+	-	?	258067	
1.2.1.85	3-methoxybenzaldehyde + NAD+ + H2O	-	Pseudomonas putida	3-methoxybenzoate + NADH + H+	-	?	403743	
1.2.1.85	3-methylbenzaldehyde + NAD+ + H2O	-	Pseudomonas putida	3-methylbenzoate + NADH + H+	-	?	403779	
1.2.1.85	3-nitrobenzaldehyde + NAD+ + H2O	-	Pseudomonas putida	3-nitrobenzoate + NADH + H+	-	?	403760	
1.2.1.85	4-methylbenzaldehyde + NAD+ + H2O	-	Pseudomonas putida	4-methylbenzoate + NADH + H+	-	?	420059	
1.2.1.85	5-chloro-2-hydroxymuconic semialdehyde + NAD+ + H2O	-	Pseudomonas sp.	5-chloro-2-hydroxymuconic acid + NADH + H+	-	?	420100	
1.2.1.85	5-chloro-2-hydroxymuconic semialdehyde + NAD+ + H2O	-	Pseudomonas sp. S-47	5-chloro-2-hydroxymuconic acid + NADH + H+	-	?	420100	
1.2.1.85	acetaldehyde + NAD+ + H2O	113% activity compared to 2-hydroxymuconate-6-semialdehyde	Bordetella sp.	acetate + NADH + H+	-	?	189125	
1.2.1.85	benzaldehyde + NAD+ + H2O	weak substrate	Pseudomonas putida	benzoate + NADH + H+	-	?	189127	
1.2.1.85	benzaldehyde + NAD+ + H2O	25% activity compared to 2-hydroxymuconate-6-semialdehyde	Bordetella sp.	benzoate + NADH + H+	-	?	189127	
1.2.1.85	butyraldehyde + NAD+ + H2O	138% activity compared to 2-hydroxymuconate-6-semialdehyde	Bordetella sp.	butanoate + NADH + H+	-	?	414400	
1.2.1.85	formaldehyde + NAD+ + H2O	16.5% activity compared to 2-hydroxymuconate-6-semialdehyde	Bordetella sp.	formate + NADH + H+	-	?	231679	
1.2.1.85	additional information	enzyme NahI is highly specific for its biological substrate, 2-hydroxymuconate semialdehyde, no activity with salicylaldehyde, another intermediate in the naphthalene-degradation pathway	Pseudomonas putida	?	-	?	89	
1.2.1.85	additional information	enzyme NahI is highly specific for its biological substrate, 2-hydroxymuconate semialdehyde, no activity with salicylaldehyde, another intermediate in the naphthalene-degradation pathway	Pseudomonas putida G7	?	-	?	89	
1.2.1.85	propionaldehyde + NAD+ + H2O	107% activity compared to 2-hydroxymuconate-6-semialdehyde	Bordetella sp.	propanoate + NADH + H+	-	?	421224