1.14.14.90	3'-hydroxyformononetin + [reduced NADPH-hemoprotein reductase] + O2	4.7% of the activity with biochanin A	Medicago truncatula	2',3'-dihydroxyformononetin + [oxidized NADPH-hemoprotein reductase] + H2O	-	?	375740	
1.14.14.90	biochanin A + [reduced NADPH-hemoprotein reductase] + O2	-	Medicago truncatula	2'-hydroxybiochanin A + [oxidized NADPH-hemoprotein reductase] + H2O	-	?	186718	
1.14.14.90	daidzein + [reduced NADPH-hemoprotein reductase] + O2	-	Glycine max	2'-hydroxydaidzein + [oxidized NADPH-hemoprotein reductase] + H2O	-	?	186719	
1.14.14.90	daidzein + [reduced NADPH-hemoprotein reductase] + O2	-	Glycyrrhiza echinata	2'-hydroxydaidzein + [oxidized NADPH-hemoprotein reductase] + H2O	-	?	186719	
1.14.14.90	daidzein + [reduced NADPH-hemoprotein reductase] + O2	18.6% of the activity with biochanin A	Medicago truncatula	2'-hydroxydaidzein + [oxidized NADPH-hemoprotein reductase] + H2O	+ low concentrations of 3'-hydroxydaidzein	?	186719	
1.14.14.90	daidzein + [reduced NADPH-hemoprotein reductase] + O2	-	Glycyrrhiza echinata L.	2'-hydroxydaidzein + [oxidized NADPH-hemoprotein reductase] + H2O	-	?	186719	
1.14.14.90	formononetin + [reduced NADPH-hemoprotein reductase] + O2	-	Glycyrrhiza echinata	2'-hydroxyformononetin + [oxidized NADPH-hemoprotein reductase] + H2O	-	?	186717	
1.14.14.90	formononetin + [reduced NADPH-hemoprotein reductase] + O2	37.2% of the activity with biochanin A	Medicago truncatula	2'-hydroxyformononetin + [oxidized NADPH-hemoprotein reductase] + H2O	-	?	186717	
1.14.14.90	formononetin + [reduced NADPH-hemoprotein reductase] + O2	-	Glycyrrhiza echinata L.	2'-hydroxyformononetin + [oxidized NADPH-hemoprotein reductase] + H2O	-	?	186717	
1.14.14.90	genistein + [reduced NADPH-hemoprotein reductase] + O2	-	Glycine max	2'-hydroxygenistein + [oxidized NADPH-hemoprotein reductase] + H2O	-	?	186720	
1.14.14.90	genistein + [reduced NADPH-hemoprotein reductase] + O2	-	Glycyrrhiza echinata	2'-hydroxygenistein + [oxidized NADPH-hemoprotein reductase] + H2O	-	?	186720	
1.14.14.90	genistein + [reduced NADPH-hemoprotein reductase] + O2	10.2% of the activity with biochanin A	Medicago truncatula	2'-hydroxygenistein + [oxidized NADPH-hemoprotein reductase] + H2O	+ low concentrations of 3'-hydroxygenistaien	?	186720	
1.14.14.90	genistein + [reduced NADPH-hemoprotein reductase] + O2	-	Glycyrrhiza echinata L.	2'-hydroxygenistein + [oxidized NADPH-hemoprotein reductase] + H2O	-	?	186720	
1.14.14.90	additional information	the enzyme is essential for isoflavan pterocarpan phytoalexin biosynthesis	Lotus japonicus	?	-	?	89	
1.14.14.90	additional information	the enzyme is involved in biosynthesis of flavonoids	Glycyrrhiza echinata	?	-	?	89	
1.14.14.90	additional information	the enzyme is involved in the biosynthesis of isoflavonoid-derived antimicrobial compounds of legumes	Glycyrrhiza echinata	?	-	?	89	
1.14.14.90	additional information	no activity with isoformononetin, prunetin, 6,7,4'-trihydroxyisoflavone, 3',4',7-trihydroxyisoflavone	Medicago truncatula	?	-	?	89	
1.14.14.90	additional information	no activity with naringenin (5,7,4'-trihydroxyflavanone), liquiritigenin (7,4'-dihydroxyflavanone), trans-cinnamic acid and 4-coumaric acid	Glycyrrhiza echinata	?	-	?	89	
1.14.14.90	additional information	no activity with formononetin, cf. EC 1.14.13.53, 4'-methoxyisoflavone 2'-hydroxylase	Glycine max	?	-	?	89	
1.14.14.90	additional information	the enzyme is involved in the biosynthesis of isoflavonoid-derived antimicrobial compounds of legumes	Glycyrrhiza echinata L.	?	-	?	89	
1.14.14.90	additional information	no activity with naringenin (5,7,4'-trihydroxyflavanone), liquiritigenin (7,4'-dihydroxyflavanone), trans-cinnamic acid and 4-coumaric acid	Glycyrrhiza echinata L.	?	-	?	89	
1.14.14.90	additional information	the enzyme is involved in biosynthesis of flavonoids	Glycyrrhiza echinata AK-1	?	-	?	89