1.14.14.126 alpha-amyrin + 3 O2 + 3 NADPH + 3 H+ overall reaction Malus domestica ursolate + 3 NADP+ + 4 H2O - ? 444209 1.14.14.126 alpha-amyrin + 3 [reduced NADPH-hemoprotein reductase] + 3 O2 overall reaction Glycyrrhiza uralensis ursolic acid + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O - ? 442264 1.14.14.126 alpha-amyrin + 3 [reduced NADPH-hemoprotein reductase] + 3 O2 overall reaction Glycyrrhiza uralensis 308-19 ursolic acid + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O - ? 442264 1.14.14.126 alpha-amyrin + [reduced NADPH-hemoprotein reductase] + O2 - Medicago truncatula ursolate + [oxidized NADPH-hemoprotein reductase] + H2O - ? 430873 1.14.14.126 alpha-amyrin + [reduced NADPH-hemoprotein reductase] + O2 - Vitis vinifera ursolate + [oxidized NADPH-hemoprotein reductase] + H2O - ? 430873 1.14.14.126 alpha-amyrin + [reduced NADPH-hemoprotein reductase] + O2 - Glycyrrhiza uralensis uvaol + [oxidized NADPH-hemoprotein reductase] + H2O - ? 442266 1.14.14.126 alpha-amyrin + [reduced NADPH-hemoprotein reductase] + O2 - Glycyrrhiza uralensis 308-19 uvaol + [oxidized NADPH-hemoprotein reductase] + H2O - ? 442266 1.14.14.126 beta-amyrin + 3 O2 + 3 NADPH + 3 H+ - Eleutherococcus senticosus oleanolate + 3 NADP+ + 4 H2O - ? 444278 1.14.14.126 beta-amyrin + 3 O2 + 3 NADPH + 3 H+ - Solanum lycopersicum oleanolate + 3 NADP+ + 4 H2O - ? 444278 1.14.14.126 beta-amyrin + 3 O2 + 3 NADPH + 3 H+ overall reaction Malus domestica oleanolate + 3 NADP+ + 4 H2O - ? 444278 1.14.14.126 beta-amyrin + 3 [reduced NADPH-hemoprotein reductase] + 3 O2 overall reaction Eleutherococcus senticosus oleanolate + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O - ? 442324 1.14.14.126 beta-amyrin + 3 [reduced NADPH-hemoprotein reductase] + 3 O2 overall reaction Platycodon grandiflorus oleanolate + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O - ? 442324 1.14.14.126 beta-amyrin + 3 [reduced NADPH-hemoprotein reductase] + 3 O2 overall reaction Glycyrrhiza uralensis oleanolate + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O - ? 442324 1.14.14.126 beta-amyrin + 3 [reduced NADPH-hemoprotein reductase] + 3 O2 overall reaction Anemone flaccida oleanolate + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O - ? 442324 1.14.14.126 beta-amyrin + 3 [reduced NADPH-hemoprotein reductase] + 3 O2 overall reaction Ocimum basilicum oleanolate + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O - ? 442324 1.14.14.126 beta-amyrin + 3 [reduced NADPH-hemoprotein reductase] + 3 O2 - Kalopanax septemlobus oleanolate + [oxidized NADPH-hemoprotein reductase] + 4 H2O - ? 442325 1.14.14.126 beta-amyrin + O2 + NADPH + H+ - Eleutherococcus senticosus erythrodiol + NADP+ + H2O - ? 444279 1.14.14.126 beta-amyrin + O2 + NADPH + H+ - Solanum lycopersicum erythrodiol + NADP+ + H2O - ? 444279 1.14.14.126 beta-amyrin + [reduced NADPH-hemoprotein reductase] + O2 - Panax ginseng oleanolate + [oxidized NADPH-hemoprotein reductase] + H2O - ? 430907 1.14.14.126 beta-amyrin + [reduced NADPH-hemoprotein reductase] + O2 - Medicago truncatula oleanolate + [oxidized NADPH-hemoprotein reductase] + H2O - ? 430907 1.14.14.126 beta-amyrin + [reduced NADPH-hemoprotein reductase] + O2 - Vitis vinifera oleanolate + [oxidized NADPH-hemoprotein reductase] + H2O - ? 430907 1.14.14.126 beta-amyrin + [reduced NADPH-hemoprotein reductase] + O2 production of oleanolate in grape skin Vitis vinifera oleanolate + [oxidized NADPH-hemoprotein reductase] + H2O - ? 430907 1.14.14.126 beta-amyrin + [reduced NADPH-hemoprotein reductase] + O2 the enzyme is involved in the biosynthesis of oleanane-type triterpenoids, such as ginsenoside Ro Panax ginseng oleanolate + [oxidized NADPH-hemoprotein reductase] + H2O - ? 430907 1.14.14.126 beta-amyrin + [reduced NADPH-hemoprotein reductase] + O2 - Medicago truncatula erythrodiol + [oxidized NADPH-hemoprotein reductase] + H2O - ? 431686 1.14.14.126 beta-amyrin + [reduced NADPH-hemoprotein reductase] + O2 - Vitis vinifera erythrodiol + [oxidized NADPH-hemoprotein reductase] + H2O - ? 431686 1.14.14.126 beta-amyrin + [reduced NADPH-hemoprotein reductase] + O2 - Eleutherococcus senticosus erythrodiol + [oxidized NADPH-hemoprotein reductase] + H2O - ? 431686 1.14.14.126 beta-amyrin + [reduced NADPH-hemoprotein reductase] + O2 - Platycodon grandiflorus erythrodiol + [oxidized NADPH-hemoprotein reductase] + H2O - ? 431686 1.14.14.126 beta-amyrin + [reduced NADPH-hemoprotein reductase] + O2 - Glycyrrhiza uralensis erythrodiol + [oxidized NADPH-hemoprotein reductase] + H2O - ? 431686 1.14.14.126 beta-amyrin + [reduced NADPH-hemoprotein reductase] + O2 - Ocimum basilicum erythrodiol + [oxidized NADPH-hemoprotein reductase] + H2O - ? 431686 1.14.14.126 beta-amyrin + [reduced NADPH-hemoprotein reductase] + O2 - Glycyrrhiza uralensis 308-19 erythrodiol + [oxidized NADPH-hemoprotein reductase] + H2O - ? 431686 1.14.14.126 beta-amyrin + [reduced NADPH-hemoprotein reductase] + O2 hydroxylation at C-16beta Platycodon grandiflorus maniladiol + [oxidized NADPH-hemoprotein reductase] + H2O - ? 442329 1.14.14.126 beta-amyrin + [reduced NADPH-hemoprotein reductase] + O2 hydroxylation at C-28 Platycodon grandiflorus oleanolic acid + [oxidized NADPH-hemoprotein reductase] + H2O - ? 442330 1.14.14.126 betulin + [reduced NADPH-hemoprotein reductase] + O2 - Glycyrrhiza uralensis betulinic aldehyde + [oxidized NADPH-hemoprotein reductase] + H2O - ? 442339 1.14.14.126 betulinic aldehyde + [reduced NADPH-hemoprotein reductase] + O2 - Glycyrrhiza uralensis betulinic acid + [oxidized NADPH-hemoprotein reductase] + H2O - ? 442341 1.14.14.126 erythrodiol + [reduced NADPH-hemoprotein reductase] + O2 - Medicago truncatula oleanolic aldehyde + [oxidized NADPH-hemoprotein reductase] + H2O - ? 431840 1.14.14.126 erythrodiol + [reduced NADPH-hemoprotein reductase] + O2 - Vitis vinifera oleanolic aldehyde + [oxidized NADPH-hemoprotein reductase] + H2O - ? 431840 1.14.14.126 erythrodiol + [reduced NADPH-hemoprotein reductase] + O2 - Eleutherococcus senticosus oleanolate + [oxidized NADPH-hemoprotein reductase] + H2O - ? 442404 1.14.14.126 erythrodiol + [reduced NADPH-hemoprotein reductase] + O2 - Platycodon grandiflorus oleanolic aldehyde + [oxidized NADPH-hemoprotein reductase] + 2 H2O - ? 442405 1.14.14.126 erythrodiol + [reduced NADPH-hemoprotein reductase] + O2 - Glycyrrhiza uralensis oleanolic aldehyde + [oxidized NADPH-hemoprotein reductase] + 2 H2O - ? 442405 1.14.14.126 erythrodiol + [reduced NADPH-hemoprotein reductase] + O2 - Ocimum basilicum oleanolic aldehyde + [oxidized NADPH-hemoprotein reductase] + 2 H2O - ? 442405 1.14.14.126 germanicol + 3 O2 + 3 NADPH + 3 H+ overall reaction Malus domestica morolate + 3 NADP+ + 4 H2O - ? 444335 1.14.14.126 lupeol + 3 O2 + 3 NADPH + 3 H+ overall reaction Malus domestica betulinate + 3 NADP+ + 4 H2O - ? 444360 1.14.14.126 lupeol + 3 [reduced NADPH-hemoprotein reductase] + 3 O2 - Glycyrrhiza uralensis betulin + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O - ? 442467 1.14.14.126 lupeol + 3 [reduced NADPH-hemoprotein reductase] + 3 O2 overall reaction Glycyrrhiza uralensis betulinic acid + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O - ? 442468 1.14.14.126 lupeol + [reduced NADPH-hemoprotein reductase] + O2 - Medicago truncatula betulinate + [oxidized NADPH-hemoprotein reductase] + H2O - ? 431057 1.14.14.126 lupeol + [reduced NADPH-hemoprotein reductase] + O2 - Vitis vinifera betulinate + [oxidized NADPH-hemoprotein reductase] + H2O - ? 431057 1.14.14.126 lupeol + [reduced NADPH-hemoprotein reductase] + O2 lupane saponin biosynthesis in grape Vitis vinifera betulinate + [oxidized NADPH-hemoprotein reductase] + H2O - ? 431057 1.14.14.126 maniladiol + [reduced NADPH-hemoprotein reductase] + O2 - Platycodon grandiflorus cochalic acid + [oxidized NADPH-hemoprotein reductase] + H2O - ? 442471 1.14.14.126 maniladiol + [reduced NADPH-hemoprotein reductase] + O2 hydroxylation at C-28 Platycodon grandiflorus cochalic acid + [oxidized NADPH-hemoprotein reductase] + H2O - ? 442471 1.14.14.126 additional information CYP716A141 is capable of catalyzing oxidation at both C-16beta and C-28, metabolites analysis by mass spectrometry Platycodon grandiflorus ? - ? 89 1.14.14.126 additional information GC-MS metabolite identification Ocimum basilicum ? - ? 89 1.14.14.126 additional information hydroxylation of beta-amyrin at C-16beta is the main activity of enzyme CYP716A141, but CYP716A141 is capable of catalyzing oxidation at both C-16beta and C-28, metabolites analysis by mass spectrometry. The enzyme hydroxylates oleanolic acid, beta-amyrin, and maniladiol Platycodon grandiflorus ? - ? 89 1.14.14.126 additional information metabolite identification by mass spectrometry Anemone flaccida ? - ? 89 1.14.14.126 additional information the enzyme catalyzes a three-step oxidation reaction at the C-28 position of beta-amyrin, alpha-amyrin, and lupeol to produce oleanolic acid, ursolic acid, and betulinic acid, respectively Glycyrrhiza uralensis ? - ? 89 1.14.14.126 additional information CYP716A175 catalyses the C-28 oxidation of alpha-amyrin, beta-amyrin, lupeol and germanicol, producing ursolic acid, oleanolic acid, betulinic acid, and putatively morolic acid. CYP716A175 catalyses the three-step oxidation of triterpene backbones at the C-28 position Malus domestica ? - ? 89 1.14.14.126 additional information CYP716A244 is a beta-amyrin 28-oxidase that converts beta-amyrin into oleanolic acid via formation of erythrodiol Eleutherococcus senticosus ? - ? 89 1.14.14.126 additional information the enzyme produces erythrodiol and oleanolic acid as C-28 oxidation products of beta-amyrin Solanum lycopersicum ? - ? 89 1.14.14.126 additional information CYP716A175 catalyses the three-step oxidation of triterpene backbones at the C-28 position. CYP716A175 has a similar oxidizing capacity for the triterpene scaffold alpha-amyrin, beta-amyrin, and lupeol but not for germanicol Malus domestica ? - ? 89 1.14.14.126 additional information CYP716A244 is a beta-amyrin 28-oxidase that converts beta-amyrin into oleanolic acid via formation of erythrodiol. The enzyme is a multifunctional oxidase enzymes with beta-amyrin 28-hydroxylase, alpha-amyrin 28-hydroxylase, and lupeol 28-hydroxylase activities Eleutherococcus senticosus ? - ? 89 1.14.14.126 additional information the enzyme catalyzes a three-step oxidation reaction at the C-28 position of beta-amyrin, alpha-amyrin, and lupeol to produce oleanolic acid, ursolic acid, and betulinic acid, respectively Glycyrrhiza uralensis 308-19 ? - ? 89 1.14.14.126 oleanolic acid + [reduced NADPH-hemoprotein reductase] + O2 hydroxylation at C-16beta, reaction of EC 1.14.13.173 Platycodon grandiflorus cochalic acid + [oxidized NADPH-hemoprotein reductase] + H2O - ? 442498 1.14.14.126 oleanolic aldehyde + [reduced NADPH-hemoprotein reductase] + O2 - Medicago truncatula oleanolate + [oxidized NADPH-hemoprotein reductase] + H2O - ? 432272 1.14.14.126 oleanolic aldehyde + [reduced NADPH-hemoprotein reductase] + O2 - Vitis vinifera oleanolate + [oxidized NADPH-hemoprotein reductase] + H2O - ? 432272 1.14.14.126 oleanolic aldehyde + [reduced NADPH-hemoprotein reductase] + O2 - Platycodon grandiflorus oleanolate + [oxidized NADPH-hemoprotein reductase] + H2O - ? 432272 1.14.14.126 oleanolic aldehyde + [reduced NADPH-hemoprotein reductase] + O2 - Glycyrrhiza uralensis oleanolate + [oxidized NADPH-hemoprotein reductase] + H2O - ? 432272 1.14.14.126 oleanolic aldehyde + [reduced NADPH-hemoprotein reductase] + O2 - Ocimum basilicum oleanolate + [oxidized NADPH-hemoprotein reductase] + H2O - ? 432272 1.14.14.126 oleanolic aldehyde + [reduced NADPH-hemoprotein reductase] + O2 - Glycyrrhiza uralensis 308-19 oleanolate + [oxidized NADPH-hemoprotein reductase] + H2O - ? 432272 1.14.14.126 ursolic aldehyde + [reduced NADPH-hemoprotein reductase] + O2 - Glycyrrhiza uralensis ursolic acid + [oxidized NADPH-hemoprotein reductase] + H2O - ? 442589 1.14.14.126 uvaol + [reduced NADPH-hemoprotein reductase] + O2 - Glycyrrhiza uralensis ursolic aldehyde + [oxidized NADPH-hemoprotein reductase] + H2O - ? 442591 1.14.14.126 beta-amyrin + [reduced NADPH-hemoprotein reductase] + O2 overall reaction Medicago truncatula oleanolate + [oxidized NADPH-hemoprotein reductase] + H2O CYP716A12 mainly catalyzes the sequential three-step oxidation at the C-28 position necessary to transform beta-amyrin into oleanolic acid ? 430907