1.14.13.230 butane + NADH + H+ + O2 - Thauera butanivorans butan-1-ol + NAD+ + H2O - ? 436417 1.14.13.230 butane + NADH + H+ + O2 - Thauera butanivorans butan-1-ol + NAD+ + H2O about 83% butan-1-ol plus 17% butan-2-ol, in presence and absence of regulatory subunit BmoB ? 436417 1.14.13.230 butane + NADH + H+ + O2 - Thauera butanivorans butan-1-ol + NAD+ + H2O formation of about 3.6% of butan-2-ol ? 436417 1.14.13.230 butane + NADH + H+ + O2 - Thauera butanivorans ATCC 43655 butan-1-ol + NAD+ + H2O - ? 436417 1.14.13.230 butane + NADH + H+ + O2 - Thauera butanivorans ATCC 43655 butan-1-ol + NAD+ + H2O about 83% butan-1-ol plus 17% butan-2-ol, in presence and absence of regulatory subunit BmoB ? 436417 1.14.13.230 butane + NADH + H+ + O2 - Thauera butanivorans butan-2-ol + NAD+ + H2O - ? 437244 1.14.13.230 butane + NADH + H+ + O2 - Thauera butanivorans ATCC 43655 butan-2-ol + NAD+ + H2O - ? 437244 1.14.13.230 ethane + NADH + H+ + O2 - Thauera butanivorans ethanol + NAD+ + H2O - ? 437533 1.14.13.230 ethane + NADH + H+ + O2 - Thauera butanivorans ATCC 43655 ethanol + NAD+ + H2O - ? 437533 1.14.13.230 hexane + NADH + H+ + O2 - Thauera butanivorans hexan-1-ol + NAD+ + H2O about 90% hexan-1-ol plus 10% hexan-2-ol in absence, and 86% hexan-1-ol plus 14% hexan-2-ol in presence of regulatory subunit BmoB, respectively ? 437608 1.14.13.230 hexane + NADH + H+ + O2 - Thauera butanivorans ATCC 43655 hexan-1-ol + NAD+ + H2O about 90% hexan-1-ol plus 10% hexan-2-ol in absence, and 86% hexan-1-ol plus 14% hexan-2-ol in presence of regulatory subunit BmoB, respectively ? 437608 1.14.13.230 isobutane + NADH + H+ + O2 - Thauera butanivorans 2-methyl-1-propanol + NAD+ + H2O about 95% 2-methyl-1-propanol plus 5% 2-methyl-2-propanol in absence, and 96% 2-methyl-1-propanol plus 4% 2-methyl-2-propanol in presence of regulatory subunit BmoB, respectively ? 437621 1.14.13.230 isobutane + NADH + H+ + O2 - Thauera butanivorans ATCC 43655 2-methyl-1-propanol + NAD+ + H2O about 95% 2-methyl-1-propanol plus 5% 2-methyl-2-propanol in absence, and 96% 2-methyl-1-propanol plus 4% 2-methyl-2-propanol in presence of regulatory subunit BmoB, respectively ? 437621 1.14.13.230 isopentane + NADH + H+ + O2 - Thauera butanivorans 3-methyl-1-butanol + 2-methyl-1-butanol + NAD+ + H2O about 63% 3-methyl-1-butanol plus 31% 2-methyl-1-butanol + 2% 3-methyl-2-butanol + 4% 2-methyl-2-butanol in absence, and 64% 3-methyl-1-butanol plus 31% 2-methyl-1-butanol + 1% 3-methyl-2-butanol + 4% 2-methyl-2-butanol in presence of regulatory subunit BmoB, respectively ? 437625 1.14.13.230 isopentane + NADH + H+ + O2 - Thauera butanivorans ATCC 43655 3-methyl-1-butanol + 2-methyl-1-butanol + NAD+ + H2O about 63% 3-methyl-1-butanol plus 31% 2-methyl-1-butanol + 2% 3-methyl-2-butanol + 4% 2-methyl-2-butanol in absence, and 64% 3-methyl-1-butanol plus 31% 2-methyl-1-butanol + 1% 3-methyl-2-butanol + 4% 2-methyl-2-butanol in presence of regulatory subunit BmoB, respectively ? 437625 1.14.13.230 methane + NADH + H+ + O2 - Thauera butanivorans methanol + NAD+ + H2O - ? 397746 1.14.13.230 methane + NADH + H+ + O2 - Thauera butanivorans ATCC 43655 methanol + NAD+ + H2O - ? 397746 1.14.13.230 additional information the reconstituted enzyme complex oxidizes C3-C6 linear and branched aliphatic alkanes. Regiospecificity is strongly biased towards primary hydroxylation, with 80% of the hydroxylations occurring at the terminal carbon atom. The hydrolase component BMOH is capable of ethene oxidation when supplied with H2O2 and ethene Thauera butanivorans ? - ? 89 1.14.13.230 additional information the reconstituted enzyme complex oxidizes C3-C6 linear and branched aliphatic alkanes. Regiospecificity is strongly biased towards primary hydroxylation, with 80% of the hydroxylations occurring at the terminal carbon atom. The hydrolase component BMOH is capable of ethene oxidation when supplied with H2O2 and ethene Thauera butanivorans ATCC 43655 ? - ? 89 1.14.13.230 pentane + NADH + H+ + O2 - Thauera butanivorans pentan-1-ol + NAD+ + H2O - ? 437812 1.14.13.230 pentane + NADH + H+ + O2 - Thauera butanivorans pentan-1-ol + NAD+ + H2O about 80% pentan-1-ol plus 13% pentan-2-ol and 7% pentan-3-ol in absence, and 88% pentan-1-ol plus 11% pentan-2-ol and 1% pentan-3-ol in presence of regulatory subunit BmoB, respectively ? 437812 1.14.13.230 pentane + NADH + H+ + O2 - Thauera butanivorans ATCC 43655 pentan-1-ol + NAD+ + H2O - ? 437812 1.14.13.230 pentane + NADH + H+ + O2 - Thauera butanivorans pentan-2-ol + NAD+ + H2O - ? 437813 1.14.13.230 pentane + NADH + H+ + O2 - Thauera butanivorans pentan-3-ol + NAD+ + H2O - ? 437814 1.14.13.230 propane + NADH + H+ + O2 - Thauera butanivorans propan-1-ol + NAD+ + H2O - ? 437834 1.14.13.230 propane + NADH + H+ + O2 - Thauera butanivorans propan-1-ol + NAD+ + H2O about 82% propan-1-ol plus 18% propan-2-ol, in presence and absence of regulatory subunit BmoB ? 437834 1.14.13.230 propane + NADH + H+ + O2 - Thauera butanivorans propan-2-ol + NAD+ + H2O - ? 437840