1.13.12.9 beta-2-thienyl-DL-Ala + O2 - Pseudomonas sp. ? + CO2 + H2O - ? 288814 1.13.12.9 beta-2-thienyl-DL-Ala + O2 - Pseudomonas sp. ? + NH3 + H2O2 - ? 288815 1.13.12.9 beta-2-thienyl-DL-Ala + O2 - Pseudomonas sp. P-501 ? + NH3 + H2O2 - ? 288815 1.13.12.9 DL-m-Tyr + O2 - Pseudomonas sp. ? + CO2 + H2O - ? 288810 1.13.12.9 DL-m-Tyr + O2 - Pseudomonas sp. ? + NH3 + H2O2 - ? 288811 1.13.12.9 DL-o-Tyr + O2 - Pseudomonas sp. ? + CO2 + H2O - ? 288808 1.13.12.9 DL-o-Tyr + O2 - Pseudomonas sp. P-501 ? + CO2 + H2O - ? 288808 1.13.12.9 DL-o-Tyr + O2 - Pseudomonas sp. ? + NH3 + H2O2 - ? 288809 1.13.12.9 L-Ala + O2 9% of the activity with L-Phe Pseudomonas sp. ? + CO2 + H2O - ? 288820 1.13.12.9 L-Ile + O2 8% of the activity with L-Phe Pseudomonas sp. ? + CO2 + H2O - ? 288821 1.13.12.9 L-Met + O2 - Pseudomonas sp. ? + CO2 + H2O - ? 288816 1.13.12.9 L-Met + O2 19% of the activity with L-Phe Pseudomonas sp. ? + CO2 + H2O - ? 288816 1.13.12.9 L-Met + O2 19% of the activity with L-Phe Pseudomonas sp. P-501 ? + CO2 + H2O - ? 288816 1.13.12.9 L-Met + O2 - Pseudomonas sp. P-501 ? + CO2 + H2O - ? 288816 1.13.12.9 L-Met + O2 - Pseudomonas sp. 2-oxo-methiolbutyrate + NH3 + H2O2 - ? 288817 1.13.12.9 L-norleucine + O2 - Pseudomonas sp. ? + NH3 + H2O2 - ? 288818 1.13.12.9 L-Phe + O2 - Pseudomonas sp. 2-phenylacetamide + CO2 + H2O - ? 288804 1.13.12.9 L-Phe + O2 - Pseudomonas sp. 2-phenylacetamide + CO2 + H2O enzyme produces both beta-phenylpyruvate and alpha-phenylacetamide from L-Phe: 0.2 mol each of beta-phenylpyruvate, ammonia and H2O2 and 0.8 mol each of alpha-phenylacetamide and CO2 under aerobic conditions ? 288804 1.13.12.9 L-Phe + O2 highly specific for L-phenylalanine Pseudomonas sp. 2-phenylacetamide + CO2 + H2O - ? 288804 1.13.12.9 L-Phe + O2 wild-type PAOpt produces higher amounts of phenylacetamide (PAM) than phenylpyruvate (PPV) as expected, but phenylacetamide is not produced by the R143K and Y536F mutants Pseudomonas sp. 2-phenylacetamide + CO2 + H2O - ? 288804 1.13.12.9 L-Phe + O2 - Pseudomonas sp. P-501 2-phenylacetamide + CO2 + H2O - ? 288804 1.13.12.9 L-Phe + O2 - Pseudomonas sp. P-501 2-phenylacetamide + CO2 + H2O enzyme produces both beta-phenylpyruvate and alpha-phenylacetamide from L-Phe: 0.2 mol each of beta-phenylpyruvate, ammonia and H2O2 and 0.8 mol each of alpha-phenylacetamide and CO2 under aerobic conditions ? 288804 1.13.12.9 L-Phe + O2 highly specific for L-phenylalanine Pseudomonas sp. P-501 2-phenylacetamide + CO2 + H2O - ? 288804 1.13.12.9 L-Phe + O2 wild-type PAOpt produces higher amounts of phenylacetamide (PAM) than phenylpyruvate (PPV) as expected, but phenylacetamide is not produced by the R143K and Y536F mutants Pseudomonas sp. P-501 2-phenylacetamide + CO2 + H2O - ? 288804 1.13.12.9 L-Phe + O2 - Pseudomonas sp. beta-phenylpyruvate + NH3 + H2O2 - ? 288805 1.13.12.9 L-Phe + O2 enzyme produces both beta-phenylpyruvate and alpha-phenylacetamide from L-Phe: 0.2 mol each of beta-phenylpyruvate, ammonia and H2O2 and 0.8 mol each of alpha-phenylacetamide and CO2 under aerobic conditions Pseudomonas sp. beta-phenylpyruvate + NH3 + H2O2 - ? 288805 1.13.12.9 L-Phe + O2 enzyme produces both beta-phenylpyruvate and alpha-phenylacetamide from L-Phe: 0.2 mol each of beta-phenylpyruvate, ammonia and H2O2 and 0.8 mol each of alpha-phenylacetamide and CO2 under aerobic conditions Pseudomonas sp. P-501 beta-phenylpyruvate + NH3 + H2O2 - ? 288805 1.13.12.9 L-Phe + O2 - Pseudomonas sp. P-501 beta-phenylpyruvate + NH3 + H2O2 - ? 288805 1.13.12.9 L-phenylalanine + O2 - Pseudomonas sp. 2-phenylacetamide + CO2 + H2O - ? 375169 1.13.12.9 L-phenylalanine + O2 oxidative deamination and oxygenative decarboxylation of L-phenylalanine, L-tyrosine, L-tryptophan, and L-methionine Pseudomonas sp. 2-phenylacetamide + CO2 + H2O - ? 375169 1.13.12.9 L-phenylalanine + O2 oxidative deamination and oxygenative decarboxylation of L-phenylalanine, L-tyrosine, L-tryptophan, and L-methionine Pseudomonas sp. P-501 2-phenylacetamide + CO2 + H2O - ? 375169 1.13.12.9 L-phenylalanine + O2 - Pseudomonas sp. P-501 2-phenylacetamide + CO2 + H2O - ? 375169 1.13.12.9 L-Trp + O2 9% of the activity with L-Phe Pseudomonas sp. ? + CO2 + H2O - ? 288819 1.13.12.9 L-Trp + O2 9% of the activity with L-Phe Pseudomonas sp. P-501 ? + CO2 + H2O - ? 288819 1.13.12.9 L-Tyr + O2 - Pseudomonas sp. ? + CO2 + H2O - ? 288806 1.13.12.9 L-Tyr + O2 44% of the activity with L-Phe Pseudomonas sp. ? + CO2 + H2O - ? 288806 1.13.12.9 L-Tyr + O2 44% of the activity with L-Phe Pseudomonas sp. P-501 ? + CO2 + H2O - ? 288806 1.13.12.9 L-Tyr + O2 - Pseudomonas sp. P-501 ? + CO2 + H2O - ? 288806 1.13.12.9 L-Tyr + O2 - Pseudomonas sp. ? + NH3 + H2O2 - ? 288807 1.13.12.9 L-Tyr + O2 - Pseudomonas sp. P-501 ? + NH3 + H2O2 - ? 288807 1.13.12.9 additional information purple intermediates consist of the reduced enzyme and an imino acid derived from a substrate, structure determination of the intermediates by resonance Raman spectroscopy Pseudomonas sp. ? - ? 89 1.13.12.9 additional information the enzyme also catalyzes the oxidative deamination of L-methionine Pseudomonas sp. ? - ? 89 1.13.12.9 additional information purple intermediates consist of the reduced enzyme and an imino acid derived from a substrate, structure determination of the intermediates by resonance Raman spectroscopy Pseudomonas sp. P-501 ? - ? 89 1.13.12.9 additional information the enzyme also catalyzes the oxidative deamination of L-methionine Pseudomonas sp. P-501 ? - ? 89 1.13.12.9 p-fluoro-DL-Phe + O2 - Pseudomonas sp. ? + CO2 + H2O - ? 288812 1.13.12.9 p-fluoro-DL-Phe + O2 - Pseudomonas sp. ? + NH3 + H2O2 - ? 288813