1.13.11.3 2,3-dihydroxybenzoate + O2 23.6% activity compared to 3,4-dihydroxybenzoate Stenotrophomonas maltophilia ? - ? 440578 1.13.11.3 2,3-dihydroxybenzoate + O2 23.6% of the activity with protocatechuate for the free enzyme, 149.3% for the calcium alginate-immobilized enzyme, and 119.8% for the glyoxyl agarose-immobilized enzyme Stenotrophomonas maltophilia ? - ? 440578 1.13.11.3 2,4-dihydroxybenzoate + O2 43.5% activity compared to 3,4-dihydroxybenzoate Stenotrophomonas maltophilia ? - ? 440598 1.13.11.3 2,4-dihydroxybenzoate + O2 43.5% of the activity with protocatechuate for the free enzyme, 96.5% for the calcium alginate-immobilized enzyme, and 65.6% for the glyoxyl agarose-immobilized enzyme Stenotrophomonas maltophilia ? - ? 440598 1.13.11.3 2,5-dihydroxybenzoate + O2 33.46% activity compared to 3,4-dihydroxybenzoate Stenotrophomonas maltophilia ? - ? 440603 1.13.11.3 2,5-dihydroxybenzoate + O2 33.5% of the activity with protocatechuate for the free enzyme, 158.6% for the calcium alginate-immobilized enzyme, and 72.1% for the glyoxyl agarose-immobilized enzyme Stenotrophomonas maltophilia ? - ? 440603 1.13.11.3 2,6-dihydroxybenzoate + O2 30.5% of the activity with protocatechuate for the free enzyme, 90.4% for the calcium alginate-immobilized enzyme, and 36.2% for the glyoxyl agarose-immobilized enzyme Stenotrophomonas maltophilia ? - ? 440610 1.13.11.3 2,6-dihydroxybenzoate + O2 30.51% activity compared to 3,4-dihydroxybenzoate Stenotrophomonas maltophilia ? - ? 440610 1.13.11.3 3,4-dihydroxybenzoate + O2 - Aspergillus niger 3-carboxy-cis,cis-muconate - ? 288914 1.13.11.3 3,4-dihydroxybenzoate + O2 - Pseudomonas aeruginosa 3-carboxy-cis,cis-muconate - ? 288914 1.13.11.3 3,4-dihydroxybenzoate + O2 - Pseudomonas sp. 3-carboxy-cis,cis-muconate - ? 288914 1.13.11.3 3,4-dihydroxybenzoate + O2 - Pseudomonas putida 3-carboxy-cis,cis-muconate - ? 288914 1.13.11.3 3,4-dihydroxybenzoate + O2 - Acinetobacter calcoaceticus 3-carboxy-cis,cis-muconate - ? 288914 1.13.11.3 3,4-dihydroxybenzoate + O2 - Corynebacterium glutamicum 3-carboxy-cis,cis-muconate - ? 288914 1.13.11.3 3,4-dihydroxybenzoate + O2 - Azotobacter vinelandii 3-carboxy-cis,cis-muconate - ? 288914 1.13.11.3 3,4-dihydroxybenzoate + O2 - Bacillus sp. (in: Bacteria) 3-carboxy-cis,cis-muconate - ? 288914 1.13.11.3 3,4-dihydroxybenzoate + O2 - Acinetobacter sp. 3-carboxy-cis,cis-muconate - ? 288914 1.13.11.3 3,4-dihydroxybenzoate + O2 - Burkholderia cepacia 3-carboxy-cis,cis-muconate - ? 288914 1.13.11.3 3,4-dihydroxybenzoate + O2 - Agrobacterium tumefaciens 3-carboxy-cis,cis-muconate - ? 288914 1.13.11.3 3,4-dihydroxybenzoate + O2 - Pseudomonas oleovorans 3-carboxy-cis,cis-muconate - ? 288914 1.13.11.3 3,4-dihydroxybenzoate + O2 - Pleurotus ostreatus 3-carboxy-cis,cis-muconate - ? 288914 1.13.11.3 3,4-dihydroxybenzoate + O2 - Stenotrophomonas maltophilia 3-carboxy-cis,cis-muconate - ? 288914 1.13.11.3 3,4-dihydroxybenzoate + O2 - Acinetobacter lwoffii 3-carboxy-cis,cis-muconate - ? 288914 1.13.11.3 3,4-dihydroxybenzoate + O2 - Brevibacterium fuscum 3-carboxy-cis,cis-muconate - ? 288914 1.13.11.3 3,4-dihydroxybenzoate + O2 - Hydrogenophaga palleronii 3-carboxy-cis,cis-muconate - ? 288914 1.13.11.3 3,4-dihydroxybenzoate + O2 - Roseobacter sp. 3-carboxy-cis,cis-muconate - ? 288914 1.13.11.3 3,4-dihydroxybenzoate + O2 - Sagittula stellata 3-carboxy-cis,cis-muconate - ? 288914 1.13.11.3 3,4-dihydroxybenzoate + O2 - Geobacillus sp. 3-carboxy-cis,cis-muconate - ? 288914 1.13.11.3 3,4-dihydroxybenzoate + O2 - Leifsonia sp. 3-carboxy-cis,cis-muconate - ? 288914 1.13.11.3 3,4-dihydroxybenzoate + O2 - Acinetobacter baylyi 3-carboxy-cis,cis-muconate - ? 288914 1.13.11.3 3,4-dihydroxybenzoate + O2 - Rhizobium sp. LMB-1 3-carboxy-cis,cis-muconate - ? 288914 1.13.11.3 3,4-dihydroxybenzoate + O2 - Rhodococcus jostii 3-carboxy-cis,cis-muconate - ? 288914 1.13.11.3 3,4-dihydroxybenzoate + O2 - Buttiauxella sp. S19-1 3-carboxy-cis,cis-muconate - ? 288914 1.13.11.3 3,4-dihydroxybenzoate + O2 best substrate Stenotrophomonas maltophilia 3-carboxy-cis,cis-muconate - ? 288914 1.13.11.3 3,4-dihydroxybenzoate + O2 very low activity with: 3,4-dihydroxyphenylacetic acid Pseudomonas aeruginosa 3-carboxy-cis,cis-muconate - ? 288914 1.13.11.3 3,4-dihydroxybenzoate + O2 specific for protocatechuate Rhizobium leguminosarum 3-carboxy-cis,cis-muconate - ? 288914 1.13.11.3 3,4-dihydroxybenzoate + O2 methylene blue cannot replace O2 as electron acceptor Neurospora crassa 3-carboxy-cis,cis-muconate - ir 288914 1.13.11.3 3,4-dihydroxybenzoate + O2 very low activity with 2-chloro-protocatechuate Burkholderia cepacia 3-carboxy-cis,cis-muconate - ? 288914 1.13.11.3 3,4-dihydroxybenzoate + O2 enzyme is active on a wide range of o-dihydroxyphenyl compounds Nocardia erythropolis 3-carboxy-cis,cis-muconate - ? 288914 1.13.11.3 3,4-dihydroxybenzoate + O2 very low activity with 3,4-dihydroxymandelic acid Pseudomonas aeruginosa 3-carboxy-cis,cis-muconate - ? 288914 1.13.11.3 3,4-dihydroxybenzoate + O2 absolute requirement for vicinal hydroxyl groups in the 3- and 4-position Brevibacterium fuscum 3-carboxy-cis,cis-muconate - ir 288914 1.13.11.3 3,4-dihydroxybenzoate + O2 very low activity with 5-bromo-protocatechuate Burkholderia cepacia 3-carboxy-cis,cis-muconate - ? 288914 1.13.11.3 3,4-dihydroxybenzoate + O2 very low activity with 5-chloro-protocatechuate Burkholderia cepacia 3-carboxy-cis,cis-muconate - ? 288914 1.13.11.3 3,4-dihydroxybenzoate + O2 very low activity with 4'-methylcatechol Pseudomonas aeruginosa 3-carboxy-cis,cis-muconate - ? 288914 1.13.11.3 3,4-dihydroxybenzoate + O2 no other substrate found Pseudomonas fluorescens 3-carboxy-cis,cis-muconate - ir 288914 1.13.11.3 3,4-dihydroxybenzoate + O2 no other substrate found Tecoma stans 3-carboxy-cis,cis-muconate - ? 288914 1.13.11.3 3,4-dihydroxybenzoate + O2 100% activity Stenotrophomonas maltophilia 3-carboxy-cis,cis-muconate - ? 288914 1.13.11.3 3,4-dihydroxybenzoate + O2 i.e. protocatechuate Pseudomonas oleovorans 3-carboxy-cis,cis-muconate - ? 288914 1.13.11.3 3,4-dihydroxybenzoate + O2 - Acinetobacter lwoffii K24 3-carboxy-cis,cis-muconate - ? 288914 1.13.11.3 3,4-dihydroxybenzoate + O2 - Agrobacterium tumefaciens S2 3-carboxy-cis,cis-muconate - ? 288914 1.13.11.3 3,4-dihydroxybenzoate + O2 - Acinetobacter baylyi Crc 3-carboxy-cis,cis-muconate - ? 288914 1.13.11.3 3,4-dihydroxybenzoate + O2 - Hydrogenophaga palleronii S1 3-carboxy-cis,cis-muconate - ? 288914 1.13.11.3 3,4-dihydroxybenzoate + O2 - Stenotrophomonas maltophilia KB2 3-carboxy-cis,cis-muconate - ? 288914 1.13.11.3 3,4-dihydroxybenzoate + O2 100% activity Stenotrophomonas maltophilia KB2 3-carboxy-cis,cis-muconate - ? 288914 1.13.11.3 3,4-dihydroxybenzoate + O2 best substrate Stenotrophomonas maltophilia KB2 3-carboxy-cis,cis-muconate - ? 288914 1.13.11.3 3,4-dihydroxybenzoate + O2 - Acinetobacter calcoaceticus 80-1 3-carboxy-cis,cis-muconate - ? 288914 1.13.11.3 3,4-dihydroxybenzoate + O2 very low activity with: 3,4-dihydroxyphenylacetic acid Pseudomonas aeruginosa B-10 3-carboxy-cis,cis-muconate - ? 288914 1.13.11.3 3,4-dihydroxybenzoate + O2 very low activity with 3,4-dihydroxymandelic acid Pseudomonas aeruginosa B-10 3-carboxy-cis,cis-muconate - ? 288914 1.13.11.3 3,4-dihydroxybenzoate + O2 very low activity with 4'-methylcatechol Pseudomonas aeruginosa B-10 3-carboxy-cis,cis-muconate - ? 288914 1.13.11.3 3,4-dihydroxybenzoate + O2 no other substrate found Pseudomonas fluorescens A.3.12. 3-carboxy-cis,cis-muconate - ir 288914 1.13.11.3 3,4-dihydroxybenzoate + O2 - Pseudomonas oleovorans KF707 3-carboxy-cis,cis-muconate - ? 288914 1.13.11.3 3,4-dihydroxybenzoate + O2 - Acinetobacter sp. ADP1 3-carboxy-cis,cis-muconate - ? 288914 1.13.11.3 3,4-dihydroxybenzoate + O2 - Acinetobacter baylyi ADPU54 3-carboxy-cis,cis-muconate - ? 288914 1.13.11.3 3,4-dihydroxybenzoate + O2 - Bacillus sp. (in: Bacteria) OS13 3-carboxy-cis,cis-muconate - ? 288914 1.13.11.3 3,4-dihydroxybenzoate + O2 spectroscopic and electronic structure study of the enzyme-substrate complex Pseudomonas putida ? - ? 288965 1.13.11.3 3,4-dihydroxybenzoate + O2 spectroscopic and electronic structure study of the enzyme-substrate complex Brevibacterium fuscum ? - ? 288965 1.13.11.3 3,4-dihydroxybenzoate + O2 - Pseudomonas putida beta-carboxy-cis,cis-muconate - ? 387313 1.13.11.3 3,4-dihydroxyhydrocinnamic acid + O2 29.5% of the activity with protocatechuate for the free enzyme, 95.0% for the calcium alginate-immobilized enzyme, and 41.1% for the glyoxyl agarose-immobilized enzyme Stenotrophomonas maltophilia ? - ? 414007 1.13.11.3 3,4-dihydroxyhydrocinnamic acid + O2 29.54% ctivity compared to 3,4-dihydroxybenzoate Stenotrophomonas maltophilia ? - ? 414007 1.13.11.3 3,4-dihydroxyhydrocinnamic acid + O2 29.54% ctivity compared to 3,4-dihydroxybenzoate Stenotrophomonas maltophilia KB2 ? - ? 414007 1.13.11.3 3,4-dihydroxyhydrocinnamic acid + O2 29.5% of the activity with protocatechuate for the free enzyme, 95.0% for the calcium alginate-immobilized enzyme, and 41.1% for the glyoxyl agarose-immobilized enzyme Stenotrophomonas maltophilia KB2 ? - ? 414007 1.13.11.3 3,4-dihydroxymandelic acid + O2 at 5.4% the rate of protocatechuic acid oxidation Nocardia erythropolis ? - ? 288923 1.13.11.3 3,4-dihydroxymandelic acid + O2 protocatechuate 3,4-dioxygenase II Hydrogenophaga palleronii ? - ? 288923 1.13.11.3 3,4-dihydroxymandelic acid + O2 protocatechuate 3,4-dioxygenase II Hydrogenophaga palleronii S1 ? - ? 288923 1.13.11.3 3,4-dihydroxyphenylacetate + O2 - Agrobacterium tumefaciens ? - ? 288926 1.13.11.3 3,4-dihydroxyphenylacetate + O2 - Hydrogenophaga palleronii ? - ? 288926 1.13.11.3 3,4-dihydroxyphenylacetate + O2 W153V protocatechuate 3,4-dioxygenase I mutant enzyme: 13% activity compared to protocatechuate, wild-type protocatechuate 3,4-dioxygenase I: 2% activity compared to protocatechuate Agrobacterium tumefaciens ? - ? 288926 1.13.11.3 3,4-dihydroxyphenylalanine + O2 at 10.7% the rate of protocatechuic acid oxidation Nocardia erythropolis ? - ? 288922 1.13.11.3 3,4-dihydroxyphenylpropionic acid + O2 - Pseudomonas aeruginosa ? - ? 288915 1.13.11.3 3,5-dihydroxybenzoate + O2 44.3% of the activity with protocatechuate for the free enzyme, 113.3% for the calcium alginate-immobilized enzyme, and 42.9% for the glyoxyl agarose-immobilized enzyme Stenotrophomonas maltophilia ? - ? 440756 1.13.11.3 3,5-dihydroxybenzoate + O2 44.33% activity compared to 3,4-dihydroxybenzoate Stenotrophomonas maltophilia ? - ? 440756 1.13.11.3 3-(3,4-dihydroxyphenyl)propionate + O2 protocatechuate 3,4-dioxygenase II Hydrogenophaga palleronii ? - ? 288927 1.13.11.3 3-methylcatechol + O2 - Agrobacterium tumefaciens 2-methylmuconate - ? 288918 1.13.11.3 3-methylcatechol + O2 - Hydrogenophaga palleronii 2-methylmuconate - ? 288918 1.13.11.3 3-methylcatechol + O2 at 0.4% the rate of protocatechuic acid oxidation Pseudomonas aeruginosa 2-methylmuconate - ? 288918 1.13.11.3 3-methylcatechol + O2 at 14.4% the rate of protocatechuic acid oxidation Nocardia erythropolis 2-methylmuconate - ? 288918 1.13.11.3 3-methylcatechol + O2 at 5% the rate of protocatechuic acid oxidation Azotobacter vinelandii 2-methylmuconate - ? 288918 1.13.11.3 3-methylcatechol + O2 - Agrobacterium tumefaciens S2 2-methylmuconate - ? 288918 1.13.11.3 3-methylcatechol + O2 - Hydrogenophaga palleronii S1 2-methylmuconate - ? 288918 1.13.11.3 4-cresol + O2 - Pseudomonas oleovorans ? - ? 387438 1.13.11.3 4-hydroxybenzoic acid + O2 - Stenotrophomonas maltophilia ? - ? 387466 1.13.11.3 4-hydroxybenzoic acid + O2 - Stenotrophomonas maltophilia KB2 ? - ? 387466 1.13.11.3 4-methylcatechol + O2 at 3.1% the rate of protocatechuic acid oxidation Nocardia erythropolis 3-methyl-cis,cis-muconate - ? 288919 1.13.11.3 4-methylcatechol + O2 at very low rates Agrobacterium tumefaciens 3-methyl-cis,cis-muconate - ? 288919 1.13.11.3 4-methylcatechol + O2 at very low rates Hydrogenophaga palleronii 3-methyl-cis,cis-muconate - ? 288919 1.13.11.3 4-methylcatechol + O2 at very low rates Agrobacterium tumefaciens S2 3-methyl-cis,cis-muconate - ? 288919 1.13.11.3 4-methylcatechol + O2 at very low rates Hydrogenophaga palleronii S1 3-methyl-cis,cis-muconate - ? 288919 1.13.11.3 4-methylcatechol + O2 - Pseudomonas oleovorans ? - ? 375900 1.13.11.3 4-sulfocatechol + O2 catalyzed by protocatechuate 3,4-dioxygenase type II only Agrobacterium tumefaciens 3-sulfomuconate - ? 288924 1.13.11.3 4-sulfocatechol + O2 catalyzed by protocatechuate 3,4-dioxygenase type II only Hydrogenophaga palleronii 3-sulfomuconate - ? 288924 1.13.11.3 4-sulfocatechol + O2 catalyzed by protocatechuate 3,4-dioxygenase type II only Agrobacterium tumefaciens S2 3-sulfomuconate - ? 288924 1.13.11.3 5-fluoro-protocatechuic acid + O2 at 2.1% the rate of protocatechuic acid oxidation Burkholderia cepacia 5-fluoro-3-carboxy-cis,cis-muconate - ? 288921 1.13.11.3 6-chloro-protocatechuate + O2 at 4.3% the rate of protocatechuic acid oxidation Burkholderia cepacia 6-chloro-3-carboxy-cis,cis-muconate - ? 288920 1.13.11.3 caffeic acid + O2 - Pseudomonas oleovorans ? - ? 261086 1.13.11.3 caffeic acid + O2 34.2% of the activity with protocatechuate for the free enzyme, 150% for the calcium alginate-immobilized enzyme, and 97% for the glyoxyl agarose-immobilized enzyme Stenotrophomonas maltophilia ? - ? 261086 1.13.11.3 caffeic acid + O2 34.21% activity compared to 3,4-dihydroxybenzoate Stenotrophomonas maltophilia ? - ? 261086 1.13.11.3 caffeic acid + O2 34.21% activity compared to 3,4-dihydroxybenzoate Stenotrophomonas maltophilia KB2 ? - ? 261086 1.13.11.3 caffeic acid + O2 34.2% of the activity with protocatechuate for the free enzyme, 150% for the calcium alginate-immobilized enzyme, and 97% for the glyoxyl agarose-immobilized enzyme Stenotrophomonas maltophilia KB2 ? - ? 261086 1.13.11.3 caffeic acid + O2 - Pseudomonas oleovorans KF707 ? - ? 261086 1.13.11.3 catechin + O2 - Pseudomonas oleovorans ? - ? 376454 1.13.11.3 catechol + O2 reaction of EC 1.13.11.1 Pseudomonas oleovorans cis,cis-muconate - ? 260573 1.13.11.3 catechol + O2 reaction of EC 1.13.11.1 Pseudomonas oleovorans KF707 cis,cis-muconate - ? 260573 1.13.11.3 catechol + O2 - Agrobacterium tumefaciens muconate - ? 288917 1.13.11.3 catechol + O2 - Hydrogenophaga palleronii muconate - ? 288917 1.13.11.3 catechol + O2 - Geobacillus sp. muconate - ? 288917 1.13.11.3 catechol + O2 at 0.4% the rate of protocatechuic acid oxidation Pseudomonas aeruginosa muconate - ? 288917 1.13.11.3 catechol + O2 at 3% the rate of protocatechuic acid oxidation Azotobacter vinelandii muconate - ? 288917 1.13.11.3 catechol + O2 at 33.8% the rate of protocatechuic acid oxidation Nocardia erythropolis muconate - ? 288917 1.13.11.3 catechol + O2 - Agrobacterium tumefaciens S2 muconate - ? 288917 1.13.11.3 catechol + O2 - Hydrogenophaga palleronii S1 muconate - ? 288917 1.13.11.3 catechol + O2 at 0.4% the rate of protocatechuic acid oxidation Pseudomonas aeruginosa B-10 muconate - ? 288917 1.13.11.3 gallate + O2 - Pseudomonas oleovorans ? - ? 376814 1.13.11.3 L-DOPA + O2 - Pseudomonas oleovorans ? - ? 386533 1.13.11.3 additional information benzoate induces expression of protocatechuate 3,4-dioxygenase Chromohalobacter sp. HS-2 ? - ? 89 1.13.11.3 additional information no activity with gentisate Geobacillus sp. ? - ? 89 1.13.11.3 additional information enzyme involved in last ring fission in naphtalene degradation, intermediates confirm proceeding through protocatechuic acid via ortho-claevage pathway Geobacillus sp. ? - ? 89 1.13.11.3 additional information strain KB2 degrades 13 mM 3,4-dihydroxybenzoate, 10 mM benzoic acid and 12 mM phenol within 24 h of incubation Stenotrophomonas maltophilia ? - ? 89 1.13.11.3 additional information the enzyme is active on a wide range of phenyl compounds, in contrast to the high specificity of similar enzymes from other sources Pseudomonas oleovorans ? - ? 89 1.13.11.3 additional information protocatechuate 3,4-dioxygenase from KB2 strain shows activity against various dihydroxybenzoic acids, but highest activity with primary substrate protocatechuate. Activity of the enzyme immobilized on calcium alginate increases particularly towards 2,5-dihydroxybenzoate, caffeic acid, 2,3-dihydroxybenzoate, and 3,5-dihydroxybenzoate, overview Stenotrophomonas maltophilia ? - ? 89 1.13.11.3 additional information strain KB2 degrades 13 mM 3,4-dihydroxybenzoate, 10 mM benzoic acid and 12 mM phenol within 24 h of incubation Stenotrophomonas maltophilia KB2 ? - ? 89 1.13.11.3 additional information protocatechuate 3,4-dioxygenase from KB2 strain shows activity against various dihydroxybenzoic acids, but highest activity with primary substrate protocatechuate. Activity of the enzyme immobilized on calcium alginate increases particularly towards 2,5-dihydroxybenzoate, caffeic acid, 2,3-dihydroxybenzoate, and 3,5-dihydroxybenzoate, overview Stenotrophomonas maltophilia KB2 ? - ? 89 1.13.11.3 additional information the enzyme is active on a wide range of phenyl compounds, in contrast to the high specificity of similar enzymes from other sources Pseudomonas oleovorans KF707 ? - ? 89 1.13.11.3 protocatechuate + O2 - Ruegeria pomeroyi beta-carboxy-cis-cis-muconate - ? 402889 1.13.11.3 protocatechuate + O2 - Sulfitobacter sp. beta-carboxy-cis-cis-muconate - ? 402889 1.13.11.3 protocatechuate + O2 - Roseovarius nubinhibens ISM beta-carboxy-cis-cis-muconate - ? 402889 1.13.11.3 protocatechuate + O2 - Sagittula stellata E-37 beta-carboxy-cis-cis-muconate - ? 402889 1.13.11.3 protocatechuate + O2 - Stenotrophomonas maltophilia 3-carboxy-cis,cis-muconate - ? 410127 1.13.11.3 protocatechuate + O2 - Stenotrophomonas maltophilia KB2 3-carboxy-cis,cis-muconate - ? 410127 1.13.11.3 protocatechuic acid + O2 - Geobacillus sp. 3-carboxy-cis,cis-muconic acid - ? 402890 1.13.11.3 pyrogallol + O2 - Pseudomonas oleovorans ? - ? 258703 1.13.11.3 pyrogallol + O2 at 10% the rate of protocatechuic acid oxidation Azotobacter vinelandii ? - ? 258703 1.13.11.3 pyrogallol + O2 at 36% the rate of protocatechuic acid oxidation Nocardia erythropolis ? - ? 258703 1.13.11.3 pyrogallol + O2 at 0.4% the rate of protocatechuic acid oxidation Pseudomonas aeruginosa ? - ? 258703 1.13.11.3 pyrogallol + O2 at 0.4% the rate of protocatechuic acid oxidation Pseudomonas aeruginosa B-10 ? - ? 258703 1.13.11.3 pyrogallol + O2 - Pseudomonas oleovorans KF707 ? - ? 258703 1.13.11.3 trans-3,4-dihydroxycinnamate + O2 - Agrobacterium tumefaciens ? - ? 288925 1.13.11.3 trans-3,4-dihydroxycinnamate + O2 - Hydrogenophaga palleronii ? - ? 288925 1.13.11.3 trans-3,4-dihydroxycinnamate + O2 W153V protocatechuate 3,4-dioxygenase I mutant enzyme: 35% activity compared to protocatechuate, wild-type protocatechuate 3,4-dioxygenase I: 2% activity compared to protocatechuate Agrobacterium tumefaciens ? - ? 288925 1.13.11.3 vanillic acid + O2 - Stenotrophomonas maltophilia ? - ? 390068 1.13.11.3 vanillic acid + O2 - Stenotrophomonas maltophilia KB2 ? - ? 390068