1.1.5.7 2-hexanol + pyrroloquinoline quinone 10% of the activity compared to cyclopentanol Gluconobacter frateurii 2-hexanone + pyrroloquinoline quinol - ir 403539 1.1.5.7 meso-erythritol + pyrroloquinoline quinone 100% of the activity compared to cyclopentanol Gluconobacter frateurii ? - ir 405815 1.1.5.7 (1S,2S)-trans-1,2-cyclohexanediol + pyrroloquinoline quinone 11% of the activity compared to cyclopentanol Gluconobacter frateurii ? - ir 403010 1.1.5.7 (1S,2S)-trans-1,2-cyclohexanediol + pyrroloquinoline quinone 11% of the activity compared to cyclopentanol Gluconobacter frateurii CHM 9 ? - ir 403010 1.1.5.7 1,3-butanediol + pyrroloquinoline quinone 12% of the activity compared to cyclopentanol Gluconobacter frateurii ? - ir 403183 1.1.5.7 cyclooctanol + pyrroloquinoline quinone 137% of the activity compared to cyclopentanol Gluconobacter frateurii cyclooctanone + pyrroloquinoline quinol - ir 404743 1.1.5.7 1,4-cyclohexanediol + pyrroloquinoline quinone 14% of the activity compared to cyclopentanol Gluconobacter frateurii ? - ir 403195 1.1.5.7 2,4-pentanediol + pyrroloquinoline quinone 16% of the activity compared to cyclopentanol Gluconobacter frateurii ? - ir 403383 1.1.5.7 2-methyl-2,4-pentanediol + pyrroloquinoline quinone 17% of the activity compared to cyclopentanol Gluconobacter frateurii ? - ir 403577 1.1.5.7 2-propanol + pyrroloquinoline quinone 17% of the activity compared to cyclopentanol Gluconobacter frateurii acetone + pyrroloquinoline quinol - ir 403633 1.1.5.7 cis-1,2-cyclopentanediol + pyrroloquinoline quinone 181% of the activity compared to cyclopentanol Gluconobacter frateurii ? - ir 404672 1.1.5.7 2,3-butanediol + pyrroloquinoline quinone 186% of the activity compared to cyclopentanol Gluconobacter frateurii ? - ir 403362 1.1.5.7 D-mannitol + pyrroloquinoline quinone 25% of the activity compared to cyclopentanol Gluconobacter frateurii ? - ir 404875 1.1.5.7 cis-4-cyclopentene-1,3-diol + pyrroloquinoline quinone 32% of the activity compared to cyclopentanol Gluconobacter frateurii ? - ir 404676 1.1.5.7 D-sorbitol + pyrroloquinoline quinone 34% of the activity compared to cyclopentanol Gluconobacter frateurii ? - ir 404912 1.1.5.7 ribitol + pyrroloquinoline quinone 34% of the activity compared to cyclopentanol Gluconobacter frateurii ? - ir 406578 1.1.5.7 (2R,3R)-2,3-butanediol + pyrroloquinoline quinone 41% of the activity compared to cyclopentanol Gluconobacter frateurii ? - ir 403019 1.1.5.7 2-butanol + pyrroloquinoline quinone 41% of the activity compared to cyclopentanol Gluconobacter frateurii butane-2-one + reduced pyrroloquinoline quinone - ir 395482 1.1.5.7 2-butanol + pyrroloquinoline quinone 41% of the activity compared to cyclopentanol Gluconobacter frateurii CHM 9 butane-2-one + reduced pyrroloquinoline quinone - ir 395482 1.1.5.7 glycerol + pyrroloquinoline quinone 59% of the activity compared to cyclopentanol Gluconobacter frateurii ? - ir 405312 1.1.5.7 1,2-butanediol + pyrroloquinoline quinone 63% of the activity compared to cyclopentanol Gluconobacter frateurii ? - ir 403169 1.1.5.7 1,3-cyclopentanediol + pyrroloquinoline quinone 73% of the activity compared to cyclopentanol Gluconobacter frateurii ? - ir 403186 1.1.5.7 cyclobutanol + pyrroloquinoline quinone 73% of the activity compared to cyclopentanol Gluconobacter frateurii cyclobutanone + pyrroloquinoline quinol - ir 404736 1.1.5.7 cyclohexanol + pyrroloquinoline quinone 73% of the activity compared to cyclopentanol Gluconobacter frateurii cyclohexanone + pyrroloquinoline quinol - ir 404739 1.1.5.7 (1R,2R)-trans-1,2-cyclohexanediol + pyrroloquinoline quinone 74% of the activity compared to cyclopentanol Gluconobacter frateurii ? - ir 403007 1.1.5.7 (1R,2R)-trans-1,2-cyclohexanediol + pyrroloquinoline quinone 74% of the activity compared to cyclopentanol Gluconobacter frateurii CHM 9 ? - ir 403007 1.1.5.7 3-pentanol + pyrroloquinoline quinone 74% of the activity compared to cyclopentanol Gluconobacter frateurii 3-pentanone + pyrroloquinoline quinol - ir 403770 1.1.5.7 D-arabitol + pyrroloquinoline quinone 78% of the activity compared to cyclopentanol Gluconobacter frateurii ? - ir 404777 1.1.5.7 cis-1,2-cyclohexanediol + pyrroloquinoline quinone 88% of the activity compared to cyclopentanol Gluconobacter frateurii ? - ir 404671 1.1.5.7 cyclopentanol + pyrroloquinoline quinone only pyrroloquinoline quinone is effective as electron acceptor, no activity with FAD, FMN and NAD(P)+ Gluconobacter frateurii cyclopentanone + pyrroloquinoline quinol - ir 404744 1.1.5.7 additional information the enzyme is unable to catalyze the reverse reaction of cyclic ketones or aldehydes to cyclic alcohols. This enzyme oxidizes a wide variety of cyclic alcohols. Some minor enzyme activity is found with aliphatic secondary alcohols and sugar alcohols, but not primary alcohols Gluconobacter frateurii ? - ? 89 1.1.5.7 additional information the enzyme is unable to catalyze the reverse reaction of cyclic ketones or aldehydes to cyclic alcohols. This enzyme oxidizes a wide variety of cyclic alcohols. Some minor enzyme activity is found with aliphatic secondary alcohols and sugar alcohols, but not primary alcohols Gluconobacter frateurii CHM 9 ? - ? 89 1.1.5.7 additional information the localization of the enzyme on the outer surface of the organism is advantageous to facilitate the oxidative fermentation of the cyclic alcohols. Since cyclic alcohols have some biological toxicity to living cells, according to the mechanism of the oxidative fermentation, there is no need to incorporate such toxic compounds into the cytoplasm to oxidize and pump out the oxidation products across the cytoplasmic membrane by the expense of bioenergy. The enzyme not inducible Gluconobacter frateurii ? - ? 89 1.1.5.7 additional information the localization of the enzyme on the outer surface of the organism is advantageous to facilitate the oxidative fermentation of the cyclic alcohols. Since cyclic alcohols have some biological toxicity to living cells, according to the mechanism of the oxidative fermentation, there is no need to incorporate such toxic compounds into the cytoplasm to oxidize and pump out the oxidation products across the cytoplasmic membrane by the expense of bioenergy. The enzyme not inducible Gluconobacter frateurii CHM 9 ? - ? 89