1.1.1.52 3alpha-hydroxy-5beta-cholanate + NAD+ - Escherichia sp. 3-oxo-5beta-cholanate + NADH - 188315 1.1.1.52 3alpha-hydroxy-5beta-cholanate + NAD+ i.e. lithocholic acid Escherichia sp. 3-oxo-5beta-cholanate + NADH - 188315 1.1.1.52 3alpha-hydroxy-bis-norcholanic acid + NAD+ oxidation at the same rate as hydroxycholanic acid Escherichia sp. 3-oxo-bis-norcholanic acid + NADH - 188319 1.1.1.52 3alpha-hydroxy-norcholanic acid + NAD+ oxidation at the same rate as hydroxycholanic acid Escherichia sp. 3-oxo-norcholanic acid + NADH - 188320 1.1.1.52 cholic acid + NAD+ oxidation at the same rate as hydroxycholanic acid Escherichia sp. 7alpha,12alpha-dihydroxy-3-oxo-5beta-cholan-24-oic acid + NADH - 188316 1.1.1.52 dehydrocholic acid + NADH i.e. 3,7,12-trioxocholan-24-oic acid Escherichia sp. 3-hydroxy-7,12-dihydroxy-5beta-cholan-24-oic acid + NAD+ - 188318 1.1.1.52 deoxycholic acid + NAD+ oxidation at the same rate as hydroxycholanic acid Escherichia sp. 12alpha-hydroxy-3-oxo-5beta-cholan-24-oic acid + NADH + H+ - 188317 1.1.1.52 additional information no substrates: androsterone and tetrahydrocortisone, methylation of carboxyl group or reduction to alcohol inactivates Escherichia sp. ? - 89