1.1.1.42 (2R,3S)-isocitrate + NAD+ slow reaction Streptomyces lividans 2-oxoglutarate + CO2 + NADH - ? 403020 1.1.1.42 (2R,3S)-isocitrate + NAD+ slow reaction Streptomyces lividans TK54 2-oxoglutarate + CO2 + NADH - ? 403020 1.1.1.42 (2R,3S)-isocitrate + NADP+ - Pisum sativum 2-oxoglutarate + CO2 + NADPH - ? 378784 1.1.1.42 (2R,3S)-isocitrate + NADP+ - Thermus thermophilus 2-oxoglutarate + CO2 + NADPH - ? 378784 1.1.1.42 (2R,3S)-isocitrate + NADP+ - Streptomyces lividans 2-oxoglutarate + CO2 + NADPH - ? 378784 1.1.1.42 (2R,3S)-isocitrate + NADP+ - Thermus thermophilus HB8 / ATCC 27634 / DSM 579 2-oxoglutarate + CO2 + NADPH - ? 378784 1.1.1.42 (2R,3S)-isocitrate + NADP+ - Streptomyces lividans TK54 2-oxoglutarate + CO2 + NADPH - ? 378784 1.1.1.42 2-oxoglutarate + CO2 + NADPH - Chlorobium limicola isocitrate + NADP+ - r 451058 1.1.1.42 2-oxoglutarate + CO2 + NADPH - Rhodopseudomonas palustris isocitrate + NADP+ - r 451058 1.1.1.42 2-oxoglutarate + CO2 + NADPH - Rhodopseudomonas palustris 285 isocitrate + NADP+ - r 451058 1.1.1.42 2-oxoglutarate + CO2 + NADPH + H+ - Homo sapiens isocitrate + NADP+ - r 458428 1.1.1.42 2-oxoglutarate + NADPH + H+ the reaction is catalyzed by diverse R132 mutant enzymes Homo sapiens D-2-hydroxyglutarate + NADP+ - r 458946 1.1.1.42 2-oxoglutarate + NADPH + H+ the reaction is catalyzed by mutant enzyme R132H Homo sapiens D-2-hydroxyglutarate + NADP+ - r 458946 1.1.1.42 2-oxoglutarate + NADPH + H+ the reaction is catalyzed by mutant enzymes R153H and R153C Escherichia coli 2-hydroxyglutarate + NADP+ - ? 458947 1.1.1.42 3-fluoroisocitrate + NADP+ - Sus scrofa 3-fluoro-2-oxoglutarate + NADPH + CO2 - ? 188125 1.1.1.42 3-hydroxyisocitrate + NADP+ - Sus scrofa 3-hydroxy-2-oxoglutarate + NADPH + CO2 - ? 188126 1.1.1.42 D-isocitrate + NADP+ - Saccharomyces cerevisiae 2-oxoglutarate + CO2 + NADPH - ? 370920 1.1.1.42 D-isocitrate + NADP+ - Nicotiana tabacum 2-oxoglutarate + CO2 + NADPH - r 370920 1.1.1.42 D-isocitrate + NADP+ - Plasmodium falciparum 2-oxoglutarate + CO2 + NADPH - ? 370920 1.1.1.42 D-isocitrate + NADP+ - Rattus norvegicus 2-oxoglutarate + CO2 + NADPH - ? 370920 1.1.1.42 D-isocitrate + NADP+ enzyme is an integral part of the mitochondrial TCA cycle, and it is involved in providing NADPH for reductive reactions in the cell Plasmodium falciparum 2-oxoglutarate + CO2 + NADPH - ? 370920 1.1.1.42 D-isocitrate + NADP+ the reaction is reversible for isozyme IDP2 Saccharomyces cerevisiae 2-oxoglutarate + CO2 + NADPH - r 370920 1.1.1.42 D-isocitrate + NADP+ - Arabidopsis thaliana 2-oxoglutarate + CO2 + NADPH + H+ - ? 421942 1.1.1.42 DL-isocitrate + NAD+ - Sus scrofa 2-oxoglutarate + CO2 + NADH - ? 370937 1.1.1.42 DL-isocitrate + NAD+ NAD+ is only substrate for mutant R291S/K343D/Y344I/V350A/Y390P Haloferax volcanii 2-oxoglutarate + CO2 + NADH - ? 370937 1.1.1.42 DL-isocitrate + NAD+ low activityy with NAD+ Streptomyces diastaticus 2-oxoglutarate + CO2 + NADH + H+ - ? 423427 1.1.1.42 DL-isocitrate + NAD+ low activityy with NAD+ Streptomyces diastaticus M1033 2-oxoglutarate + CO2 + NADH + H+ - ? 423427 1.1.1.42 DL-isocitrate + NADP+ - Homo sapiens 2-oxoglutarate + CO2 + NADPH - ? 370938 1.1.1.42 DL-isocitrate + NADP+ - Rattus norvegicus 2-oxoglutarate + CO2 + NADPH - ? 370938 1.1.1.42 DL-isocitrate + NADP+ - Sus scrofa 2-oxoglutarate + CO2 + NADPH - ? 370938 1.1.1.42 DL-isocitrate + NADP+ - Mycobacterium tuberculosis 2-oxoglutarate + CO2 + NADPH - ? 370938 1.1.1.42 DL-isocitrate + NADP+ - Saccharopolyspora erythraea 2-oxoglutarate + CO2 + NADPH - ? 370938 1.1.1.42 DL-isocitrate + NADP+ - Fomitopsis palustris 2-oxoglutarate + CO2 + NADPH - ? 370938 1.1.1.42 DL-isocitrate + NADP+ - Haloferax volcanii 2-oxoglutarate + CO2 + NADPH - ? 370938 1.1.1.42 DL-isocitrate + NADP+ NAD+ is not substrate for mutant R291S/K343D/Y344I/V350A/Y390P Haloferax volcanii 2-oxoglutarate + CO2 + NADPH - ? 370938 1.1.1.42 DL-isocitrate + NADP+ - Saccharopolyspora erythraea CA340 2-oxoglutarate + CO2 + NADPH - ? 370938 1.1.1.42 DL-isocitrate + NADP+ - Leishmania mexicana 2-oxoglutarate + CO2 + NADPH + H+ - ? 421958 1.1.1.42 DL-isocitrate + NADP+ - Streptomyces diastaticus 2-oxoglutarate + CO2 + NADPH + H+ - ? 421958 1.1.1.42 DL-isocitrate + NADP+ - Microcystis aeruginosa 2-oxoglutarate + CO2 + NADPH + H+ - ? 421958 1.1.1.42 DL-isocitrate + NADP+ - Trypanosoma cruzi 2-oxoglutarate + CO2 + NADPH + H+ - ir 421958 1.1.1.42 DL-isocitrate + NADP+ - Microcystis aeruginosa 2-oxoglutarate + CO2 + NADPH + H+ - r 421958 1.1.1.42 DL-isocitrate + NADP+ carboxylation of 2-oxoglutarate to produce isocitrate Trypanosoma cruzi 2-oxoglutarate + CO2 + NADPH + H+ - ir 421958 1.1.1.42 DL-isocitrate + NADP+ NADP+ is the highly preferred cofactor Streptomyces diastaticus 2-oxoglutarate + CO2 + NADPH + H+ - ? 421958 1.1.1.42 DL-isocitrate + NADP+ - Streptomyces diastaticus M1033 2-oxoglutarate + CO2 + NADPH + H+ - ? 421958 1.1.1.42 DL-isocitrate + NADP+ NADP+ is the highly preferred cofactor Streptomyces diastaticus M1033 2-oxoglutarate + CO2 + NADPH + H+ - ? 421958 1.1.1.42 Ds-isocitrate + NADP+ - Sus scrofa 2-oxoglutarate + CO2 + NADPH - ? 373141 1.1.1.42 isocitrate + APADP+ 60% activity compared to NAD+ Methylobacillus flagellatus 2-oxoglutarate + CO2 + APADPH - r 459755 1.1.1.42 isocitrate + APADP+ 60% activity compared to NAD+ Methylobacillus flagellatus ATCC51484 2-oxoglutarate + CO2 + APADPH - r 459755 1.1.1.42 isocitrate + NAD+ - Rhodomicrobium vannielii 2-oxoglutarate + CO2 + NADH + H+ - r 188122 1.1.1.42 isocitrate + NAD+ - Xylella fastidiosa 2-oxoglutarate + CO2 + NADH + H+ - ? 188122 1.1.1.42 isocitrate + NAD+ - Archaeoglobus fulgidus 2-oxoglutarate + CO2 + NADH + H+ - r 188122 1.1.1.42 isocitrate + NAD+ Km-value for NAD+ is 34fold higher compared to KM-value for NADP+ Saccharolobus solfataricus 2-oxoglutarate + CO2 + NADH + H+ - ? 188122 1.1.1.42 isocitrate + NAD+ engineered enzyme mutant D253A/S257K/K260Q/R314D/H315I/T327A, cf. EC 1.1.1.41 Bifidobacterium longum subsp. infantis 2-oxoglutarate + CO2 + NADH + H+ - r 188122 1.1.1.42 isocitrate + NAD+ low activity with mutant enzyme R322D, no activity with the wild-type enzyme Yarrowia lipolytica 2-oxoglutarate + CO2 + NADH + H+ - r 188122 1.1.1.42 isocitrate + NAD+ the reaction is catalyzed by mutant enzymes H590L/R601D/R650S and H590L/R601D Methanococcoides methylutens 2-oxoglutarate + CO2 + NADH + H+ - ? 188122 1.1.1.42 isocitrate + NAD+ low activity with mutant enzyme R322D, no activity with the wild-type enzyme Yarrowia lipolytica CLIB 122 2-oxoglutarate + CO2 + NADH + H+ - r 188122 1.1.1.42 isocitrate + NAD+ the reaction is catalyzed by mutant enzymes H590L/R601D/R650S and H590L/R601D Methanococcoides methylutens MM1 2-oxoglutarate + CO2 + NADH + H+ - ? 188122 1.1.1.42 isocitrate + NAD+ engineered enzyme mutant D253A/S257K/K260Q/R314D/H315I/T327A, cf. EC 1.1.1.41 Bifidobacterium longum subsp. infantis ATCC 15697 2-oxoglutarate + CO2 + NADH + H+ - r 188122 1.1.1.42 isocitrate + NAD+ Km-value for NAD+ is 34fold higher compared to KM-value for NADP+ Saccharolobus solfataricus DSM 1616 2-oxoglutarate + CO2 + NADH + H+ - ? 188122 1.1.1.42 isocitrate + NADP+ - Thermus aquaticus 2-oxoglutarate + NADPH + H+ + CO2 - ? 188124 1.1.1.42 isocitrate + NADP+ - Chlamydomonas reinhardtii 2-oxoglutarate + NADPH + H+ + CO2 - ? 188124 1.1.1.42 isocitrate + NADP+ - Bacillus subtilis 2-oxoglutarate + NADPH + H+ + CO2 - ? 188124 1.1.1.42 isocitrate + NADP+ - Thermus thermophilus 2-oxoglutarate + NADPH + H+ + CO2 - ? 188124 1.1.1.42 isocitrate + NADP+ - Escherichia coli 2-oxoglutarate + NADPH + H+ + CO2 - ? 188124 1.1.1.42 isocitrate + NADP+ - Homo sapiens 2-oxoglutarate + NADPH + H+ + CO2 - ? 188124 1.1.1.42 isocitrate + NADP+ - Homo sapiens 2-oxoglutarate + NADPH + H+ + CO2 - r 188124 1.1.1.42 isocitrate + NADP+ - Rattus norvegicus 2-oxoglutarate + NADPH + H+ + CO2 - ? 188124 1.1.1.42 isocitrate + NADP+ - Sus scrofa 2-oxoglutarate + NADPH + H+ + CO2 - ? 188124 1.1.1.42 isocitrate + NADP+ - Sus scrofa 2-oxoglutarate + NADPH + H+ + CO2 - r 188124 1.1.1.42 isocitrate + NADP+ - Saccharomyces cerevisiae 2-oxoglutarate + NADPH + H+ + CO2 - ? 188124 1.1.1.42 isocitrate + NADP+ - Bos taurus 2-oxoglutarate + NADPH + H+ + CO2 - ? 188124 1.1.1.42 isocitrate + NADP+ - Bombyx mori 2-oxoglutarate + NADPH + H+ + CO2 - ? 188124 1.1.1.42 isocitrate + NADP+ - Geobacillus stearothermophilus 2-oxoglutarate + NADPH + H+ + CO2 - ? 188124 1.1.1.42 isocitrate + NADP+ - Aspergillus nidulans 2-oxoglutarate + NADPH + H+ + CO2 - ? 188124 1.1.1.42 isocitrate + NADP+ - Cereibacter sphaeroides 2-oxoglutarate + NADPH + H+ + CO2 - ? 188124 1.1.1.42 isocitrate + NADP+ - Aspergillus niger 2-oxoglutarate + NADPH + H+ + CO2 - ? 188124 1.1.1.42 isocitrate + NADP+ - Phycomyces blakesleeanus 2-oxoglutarate + NADPH + H+ + CO2 - ? 188124 1.1.1.42 isocitrate + NADP+ - Pisum sativum 2-oxoglutarate + NADPH + H+ + CO2 - ? 188124 1.1.1.42 isocitrate + NADP+ - Zea mays 2-oxoglutarate + NADPH + H+ + CO2 - ? 188124 1.1.1.42 isocitrate + NADP+ - Nicotiana tabacum 2-oxoglutarate + NADPH + H+ + CO2 - ? 188124 1.1.1.42 isocitrate + NADP+ - Arabidopsis thaliana 2-oxoglutarate + NADPH + H+ + CO2 - ? 188124 1.1.1.42 isocitrate + NADP+ - Acinetobacter calcoaceticus 2-oxoglutarate + NADPH + H+ + CO2 - ? 188124 1.1.1.42 isocitrate + NADP+ - Halobacterium salinarum 2-oxoglutarate + NADPH + H+ + CO2 - ? 188124 1.1.1.42 isocitrate + NADP+ - Crithidia fasciculata 2-oxoglutarate + NADPH + H+ + CO2 - ? 188124 1.1.1.42 isocitrate + NADP+ - Mycolicibacterium phlei 2-oxoglutarate + NADPH + H+ + CO2 - ? 188124 1.1.1.42 isocitrate + NADP+ - [Brevibacterium] flavum 2-oxoglutarate + NADPH + H+ + CO2 - ? 188124 1.1.1.42 isocitrate + NADP+ - Corynebacterium glutamicum 2-oxoglutarate + NADPH + H+ + CO2 - ? 188124 1.1.1.42 isocitrate + NADP+ - Mycobacterium tuberculosis 2-oxoglutarate + NADPH + H+ + CO2 - ? 188124 1.1.1.42 isocitrate + NADP+ - Azotobacter vinelandii 2-oxoglutarate + NADPH + H+ + CO2 - ? 188124 1.1.1.42 isocitrate + NADP+ - Trypanosoma cruzi 2-oxoglutarate + NADPH + H+ + CO2 - ? 188124 1.1.1.42 isocitrate + NADP+ - Starkeya novella 2-oxoglutarate + NADPH + H+ + CO2 - ? 188124 1.1.1.42 isocitrate + NADP+ - Bacillus sp. (in: Bacteria) 2-oxoglutarate + NADPH + H+ + CO2 - ? 188124 1.1.1.42 isocitrate + NADP+ - Vibrio sp. 2-oxoglutarate + NADPH + H+ + CO2 - ? 188124 1.1.1.42 isocitrate + NADP+ - Citrobacter freundii 2-oxoglutarate + NADPH + H+ + CO2 - ? 188124 1.1.1.42 isocitrate + NADP+ - Blastocladiella emersonii 2-oxoglutarate + NADPH + H+ + CO2 - ? 188124 1.1.1.42 isocitrate + NADP+ - Rhodomicrobium vannielii 2-oxoglutarate + NADPH + H+ + CO2 - ? 188124 1.1.1.42 isocitrate + NADP+ - Vibrio parahaemolyticus 2-oxoglutarate + NADPH + H+ + CO2 - ? 188124 1.1.1.42 isocitrate + NADP+ - Synechocystis sp. 2-oxoglutarate + NADPH + H+ + CO2 - ? 188124 1.1.1.42 isocitrate + NADP+ - Mytilus edulis 2-oxoglutarate + NADPH + H+ + CO2 - ? 188124 1.1.1.42 isocitrate + NADP+ - Phormidium laminosum 2-oxoglutarate + NADPH + H+ + CO2 - ? 188124 1.1.1.42 isocitrate + NADP+ - Fundulus heteroclitus 2-oxoglutarate + NADPH + H+ + CO2 - ? 188124 1.1.1.42 isocitrate + NADP+ - Azotobacter sp. 2-oxoglutarate + NADPH + H+ + CO2 - ? 188124 1.1.1.42 isocitrate + NADP+ - Caldococcus noboribetus 2-oxoglutarate + NADPH + H+ + CO2 - ? 188124 1.1.1.42 isocitrate + NADP+ - Desulfobacter vibrioformis 2-oxoglutarate + NADPH + H+ + CO2 - ? 188124 1.1.1.42 isocitrate + NADP+ - Pinus spp. 2-oxoglutarate + NADPH + H+ + CO2 - ? 188124 1.1.1.42 isocitrate + NADP+ - Thermoleophilum minutum 2-oxoglutarate + NADPH + H+ + CO2 - ? 188124 1.1.1.42 isocitrate + NADP+ - Archaeoglobus fulgidus 2-oxoglutarate + NADPH + H+ + CO2 - ? 188124 1.1.1.42 isocitrate + NADP+ IDH1 is an enzyme that catalyzes the oxidative decarboxylation of isocitrate into alpha-ketoglutarate utilizing either NAD+ or NADP+ as cosubstrates Homo sapiens 2-oxoglutarate + NADPH + H+ + CO2 - ? 188124 1.1.1.42 isocitrate + NADP+ - Vibrio sp. ABE-1 2-oxoglutarate + NADPH + H+ + CO2 - ? 188124 1.1.1.42 isocitrate + NADP+ - Arabidopsis thaliana Columbia ecotype 2-oxoglutarate + NADPH + H+ + CO2 - ? 188124 1.1.1.42 isocitrate + NADP+ - Saccharomyces cerevisiae MMY011 2-oxoglutarate + NADPH + H+ + CO2 - ? 188124 1.1.1.42 isocitrate + NADP+ - Thermoleophilum minutum YS-4 2-oxoglutarate + NADPH + H+ + CO2 - ? 188124 1.1.1.42 isocitrate + NADP+ - Vibrio parahaemolyticus Y-4 2-oxoglutarate + NADPH + H+ + CO2 - ? 188124 1.1.1.42 isocitrate + NADP+ - Chlorobium limicola 2-oxoglutarate + CO2 + NADPH + H+ - r 370996 1.1.1.42 isocitrate + NADP+ - Mus musculus 2-oxoglutarate + CO2 + NADPH + H+ - ? 370996 1.1.1.42 isocitrate + NADP+ - Escherichia coli 2-oxoglutarate + CO2 + NADPH + H+ - ? 370996 1.1.1.42 isocitrate + NADP+ - Homo sapiens 2-oxoglutarate + CO2 + NADPH + H+ - ? 370996 1.1.1.42 isocitrate + NADP+ - Homo sapiens 2-oxoglutarate + CO2 + NADPH + H+ - r 370996 1.1.1.42 isocitrate + NADP+ - Rattus norvegicus 2-oxoglutarate + CO2 + NADPH + H+ - ? 370996 1.1.1.42 isocitrate + NADP+ - Sus scrofa 2-oxoglutarate + CO2 + NADPH + H+ - ? 370996 1.1.1.42 isocitrate + NADP+ - Saccharomyces cerevisiae 2-oxoglutarate + CO2 + NADPH + H+ - ? 370996 1.1.1.42 isocitrate + NADP+ - Aspergillus niger 2-oxoglutarate + CO2 + NADPH + H+ - r 370996 1.1.1.42 isocitrate + NADP+ - Pisum sativum 2-oxoglutarate + CO2 + NADPH + H+ - ? 370996 1.1.1.42 isocitrate + NADP+ - Pisum sativum 2-oxoglutarate + CO2 + NADPH + H+ - r 370996 1.1.1.42 isocitrate + NADP+ - Zea mays 2-oxoglutarate + CO2 + NADPH + H+ - ? 370996 1.1.1.42 isocitrate + NADP+ - Rhodopseudomonas palustris 2-oxoglutarate + CO2 + NADPH + H+ - ? 370996 1.1.1.42 isocitrate + NADP+ - Yarrowia lipolytica 2-oxoglutarate + CO2 + NADPH + H+ - ? 370996 1.1.1.42 isocitrate + NADP+ - Haloferax volcanii 2-oxoglutarate + CO2 + NADPH + H+ - ? 370996 1.1.1.42 isocitrate + NADP+ - Spirodela polyrhiza 2-oxoglutarate + CO2 + NADPH + H+ - ? 370996 1.1.1.42 isocitrate + NADP+ - Acinetobacter baumannii 2-oxoglutarate + CO2 + NADPH + H+ - ? 370996 1.1.1.42 isocitrate + NADP+ - Colwellia maris 2-oxoglutarate + CO2 + NADPH + H+ - ? 370996 1.1.1.42 isocitrate + NADP+ - Psalidodon scabripinnis 2-oxoglutarate + CO2 + NADPH + H+ - ? 370996 1.1.1.42 isocitrate + NADP+ - Xylella fastidiosa 2-oxoglutarate + CO2 + NADPH + H+ - ? 370996 1.1.1.42 isocitrate + NADP+ - Desulfotalea psychrophila 2-oxoglutarate + CO2 + NADPH + H+ - ? 370996 1.1.1.42 isocitrate + NADP+ - Rattus norvegicus 2-oxoglutarate + CO2 + NADPH + H+ - r 370996 1.1.1.42 isocitrate + NADP+ - Mus musculus 2-oxoglutarate + CO2 + NADPH + H+ - r 370996 1.1.1.42 isocitrate + NADP+ - Mycobacterium tuberculosis 2-oxoglutarate + CO2 + NADPH + H+ - ? 370996 1.1.1.42 isocitrate + NADP+ - Sus scrofa 2-oxoglutarate + CO2 + NADPH + H+ - r 370996 1.1.1.42 isocitrate + NADP+ - Archaeoglobus fulgidus 2-oxoglutarate + CO2 + NADPH + H+ - ? 370996 1.1.1.42 isocitrate + NADP+ - Thermotoga maritima 2-oxoglutarate + CO2 + NADPH + H+ - ? 370996 1.1.1.42 isocitrate + NADP+ - Saccharolobus solfataricus 2-oxoglutarate + CO2 + NADPH + H+ - ? 370996 1.1.1.42 isocitrate + NADP+ - Corynebacterium glutamicum 2-oxoglutarate + CO2 + NADPH + H+ - ? 370996 1.1.1.42 isocitrate + NADP+ - Capsicum annuum 2-oxoglutarate + CO2 + NADPH + H+ - ? 370996 1.1.1.42 isocitrate + NADP+ - Thermoplasma acidophilum 2-oxoglutarate + CO2 + NADPH + H+ - ? 370996 1.1.1.42 isocitrate + NADP+ - Staphylococcus aureus 2-oxoglutarate + CO2 + NADPH + H+ - ? 370996 1.1.1.42 isocitrate + NADP+ - Arabidopsis thaliana 2-oxoglutarate + CO2 + NADPH + H+ - r 370996 1.1.1.42 isocitrate + NADP+ - Gryllus firmus 2-oxoglutarate + CO2 + NADPH + H+ - ? 370996 1.1.1.42 isocitrate + NADP+ - Aeropyrum pernix 2-oxoglutarate + CO2 + NADPH + H+ - ? 370996 1.1.1.42 isocitrate + NADP+ - Caldococcus noboribetus 2-oxoglutarate + CO2 + NADPH + H+ - ? 370996 1.1.1.42 isocitrate + NADP+ - Colwellia psychrerythraea 2-oxoglutarate + CO2 + NADPH + H+ - r 370996 1.1.1.42 isocitrate + NADP+ - Bifidobacterium longum subsp. infantis 2-oxoglutarate + CO2 + NADPH + H+ - r 370996 1.1.1.42 isocitrate + NADP+ - Yarrowia lipolytica 2-oxoglutarate + CO2 + NADPH + H+ - r 370996 1.1.1.42 isocitrate + NADP+ - Methanococcoides methylutens 2-oxoglutarate + CO2 + NADPH + H+ - ? 370996 1.1.1.42 isocitrate + NADP+ - Methylobacillus flagellatus 2-oxoglutarate + CO2 + NADPH + H+ - r 370996 1.1.1.42 isocitrate + NADP+ - Pinus pinaster 2-oxoglutarate + CO2 + NADPH + H+ - r 370996 1.1.1.42 isocitrate + NADP+ - Edwardsiella tarda 2-oxoglutarate + CO2 + NADPH + H+ - r 370996 1.1.1.42 isocitrate + NADP+ - Triticum aestivum 2-oxoglutarate + CO2 + NADPH + H+ - ? 370996 1.1.1.42 isocitrate + NADP+ - Psychromonas marina 2-oxoglutarate + CO2 + NADPH + H+ - ? 370996 1.1.1.42 isocitrate + NADP+ - Pseudomonas psychrophila 2-oxoglutarate + CO2 + NADPH + H+ - ? 370996 1.1.1.42 isocitrate + NADP+ - marine plankton environmental sample 2-oxoglutarate + CO2 + NADPH + H+ - r 370996 1.1.1.42 isocitrate + NADP+ carbon dioxide-fixing enzyme in the reductive tricarboxylic acid cycle, preference for carboxylation reaction direction Chlorobium limicola 2-oxoglutarate + CO2 + NADPH + H+ - r 370996 1.1.1.42 isocitrate + NADP+ cytosolic enzyme is critical in fat and cholesterol biosynthesis, enzyme content correlates with adipogenesis in wild-type adipocytes and in transgenic cells Mus musculus 2-oxoglutarate + CO2 + NADPH + H+ - ? 370996 1.1.1.42 isocitrate + NADP+ enzyme has a self-regulatory mechanism of activity mimicking the phosphorylation mechanism of bacterial enzymes Homo sapiens 2-oxoglutarate + CO2 + NADPH + H+ - ? 370996 1.1.1.42 isocitrate + NADP+ isozymes are compartment interchangeable for glutamate synthesis, although mitochondrial localization has a positive impact on this function during fermentative growth Saccharomyces cerevisiae 2-oxoglutarate + CO2 + NADPH + H+ - ? 370996 1.1.1.42 isocitrate + NADP+ key enzyme of the tricarboxylic acid cycle Fomitopsis palustris 2-oxoglutarate + CO2 + NADPH + H+ - ? 370996 1.1.1.42 isocitrate + NADP+ metabolic regulation of the isozymes Spirodela polyrhiza 2-oxoglutarate + CO2 + NADPH + H+ - ? 370996 1.1.1.42 isocitrate + NADP+ metabolic regulation, involved in the tricarboxylic acid cycle Chlorobium limicola 2-oxoglutarate + CO2 + NADPH + H+ - r 370996 1.1.1.42 isocitrate + NADP+ metabolic regulation, involved in the tricarboxylic acid cycle Rhodopseudomonas palustris 2-oxoglutarate + CO2 + NADPH + H+ - r 370996 1.1.1.42 isocitrate + NADP+ the cytosolic isozyme supplies 2-oxoglutarate for the photorespiratory ammonia fixation, pyridine nucleotide contents in mitochondria and cytosol, regulation, overview Pisum sativum 2-oxoglutarate + CO2 + NADPH + H+ - r 370996 1.1.1.42 isocitrate + NADP+ overall reaction Acinetobacter baumannii 2-oxoglutarate + CO2 + NADPH + H+ - ? 370996 1.1.1.42 isocitrate + NADP+ reaction is reversible for isozymes IDP1 and IDP2 Saccharomyces cerevisiae 2-oxoglutarate + CO2 + NADPH + H+ - r 370996 1.1.1.42 isocitrate + NADP+ Ser95, Asn97, and Thr78 are involved in substrate binding Sus scrofa 2-oxoglutarate + CO2 + NADPH + H+ - ? 370996 1.1.1.42 isocitrate + NADP+ isocitrate dehydrogenase is an important NADPH-generating enzyme in the endoplasmic reticulum Rattus norvegicus 2-oxoglutarate + CO2 + NADPH + H+ - ? 370996 1.1.1.42 isocitrate + NADP+ the enzyme could have a protective antioxidant role against certain environmental stresses in plants Pisum sativum 2-oxoglutarate + CO2 + NADPH + H+ - ? 370996 1.1.1.42 isocitrate + NADP+ the enzyme shows high substrate specificity Corynebacterium glutamicum 2-oxoglutarate + CO2 + NADPH + H+ - ? 370996 1.1.1.42 isocitrate + NADP+ Km-value for NAD+ is 34fold higher compared to KM-value for NADP+ Saccharolobus solfataricus 2-oxoglutarate + CO2 + NADPH + H+ - ? 370996 1.1.1.42 isocitrate + NADP+ strict substrate and coenzyme specificity Caldococcus noboribetus 2-oxoglutarate + CO2 + NADPH + H+ - ? 370996 1.1.1.42 isocitrate + NADP+ low activity with NAD+. Km-value for NAD+ is about 140fold higher than the KM-value for NADP+ Thermotoga maritima 2-oxoglutarate + CO2 + NADPH + H+ - ? 370996 1.1.1.42 isocitrate + NADP+ 100% actiivty Methylobacillus flagellatus 2-oxoglutarate + CO2 + NADPH + H+ - r 370996 1.1.1.42 isocitrate + NADP+ - Aeropyrum pernix DSM 11879 2-oxoglutarate + CO2 + NADPH + H+ - ? 370996 1.1.1.42 isocitrate + NADP+ - Rhodopseudomonas palustris 285 2-oxoglutarate + CO2 + NADPH + H+ - ? 370996 1.1.1.42 isocitrate + NADP+ metabolic regulation, involved in the tricarboxylic acid cycle Rhodopseudomonas palustris 285 2-oxoglutarate + CO2 + NADPH + H+ - r 370996 1.1.1.42 isocitrate + NADP+ - Edwardsiella tarda EIB202 2-oxoglutarate + CO2 + NADPH + H+ - r 370996 1.1.1.42 isocitrate + NADP+ - Escherichia coli K-12 W3110 2-oxoglutarate + CO2 + NADPH + H+ - ? 370996 1.1.1.42 isocitrate + NADP+ - Yarrowia lipolytica CLIB 122 2-oxoglutarate + CO2 + NADPH + H+ - r 370996 1.1.1.42 isocitrate + NADP+ - Thermotoga maritima DSM 3109 2-oxoglutarate + CO2 + NADPH + H+ - ? 370996 1.1.1.42 isocitrate + NADP+ low activity with NAD+. Km-value for NAD+ is about 140fold higher than the KM-value for NADP+ Thermotoga maritima DSM 3109 2-oxoglutarate + CO2 + NADPH + H+ - ? 370996 1.1.1.42 isocitrate + NADP+ - Arabidopsis thaliana Col-0 2-oxoglutarate + CO2 + NADPH + H+ - r 370996 1.1.1.42 isocitrate + NADP+ - Capsicum annuum CMS-9704A 2-oxoglutarate + CO2 + NADPH + H+ - ? 370996 1.1.1.42 isocitrate + NADP+ - Methanococcoides methylutens MM1 2-oxoglutarate + CO2 + NADPH + H+ - ? 370996 1.1.1.42 isocitrate + NADP+ - Mus musculus C57BL/6 2-oxoglutarate + CO2 + NADPH + H+ - r 370996 1.1.1.42 isocitrate + NADP+ - Mycobacterium tuberculosis H37Rv 2-oxoglutarate + CO2 + NADPH + H+ - ? 370996 1.1.1.42 isocitrate + NADP+ - Colwellia psychrerythraea DSM 8813 2-oxoglutarate + CO2 + NADPH + H+ - r 370996 1.1.1.42 isocitrate + NADP+ - Bifidobacterium longum subsp. infantis ATCC 15697 2-oxoglutarate + CO2 + NADPH + H+ - r 370996 1.1.1.42 isocitrate + NADP+ - Saccharolobus solfataricus DSM 1616 2-oxoglutarate + CO2 + NADPH + H+ - ? 370996 1.1.1.42 isocitrate + NADP+ Km-value for NAD+ is 34fold higher compared to KM-value for NADP+ Saccharolobus solfataricus DSM 1616 2-oxoglutarate + CO2 + NADPH + H+ - ? 370996 1.1.1.42 isocitrate + NADP+ - Methylobacillus flagellatus ATCC51484 2-oxoglutarate + CO2 + NADPH + H+ - r 370996 1.1.1.42 isocitrate + NADP+ 100% actiivty Methylobacillus flagellatus ATCC51484 2-oxoglutarate + CO2 + NADPH + H+ - r 370996 1.1.1.42 isocitrate + NADP+ - Staphylococcus aureus ATCC 12600 2-oxoglutarate + CO2 + NADPH + H+ - ? 370996 1.1.1.42 isocitrate + NADP+ - Haloferax volcanii DSM 3757 2-oxoglutarate + CO2 + NADPH + H+ - ? 370996 1.1.1.42 isocitrate + NADP+ - Thermus thermophilus oxalosuccinate + NADPH + H+ - r 438365 1.1.1.42 isocitrate + NADP+ - Zea mays oxalosuccinate + NADPH + H+ - r 438365 1.1.1.42 isocitrate + NHDP+ 2.0% activity compared to NAD+ Methylobacillus flagellatus 2-oxoglutarate + CO2 + NHDPH - r 459757 1.1.1.42 isocitrate + NHDP+ 2.0% activity compared to NAD+ Methylobacillus flagellatus ATCC51484 2-oxoglutarate + CO2 + NHDPH - r 459757 1.1.1.42 isopropylmalate + NADP+ - Escherichia coli ? - ? 188127 1.1.1.42 additional information His319 and His315 are not responsible for enzyme Fe2+-isocitrate cleavage Sus scrofa ? - ? 89 1.1.1.42 additional information 86% of total activity in the cell, main factor for synthesis of 2-oxoglutarate. Enzyme and cytoplasmic aspartate aminotransferase are regulated oppositely and the catalytic activity of one enzyme can be stimulated concurrently with a decrease in the activity of the other Rattus norvegicus ? - ? 89 1.1.1.42 additional information during oxidative stress, enzyme activity appears to be modulated through enzymatic glutathionylation and deglutathionylation Sus scrofa ? - ? 89 1.1.1.42 additional information enzyme plays an important regulatory role in cellular defense against oxidative stress and in senescence Mus musculus ? - ? 89 1.1.1.42 additional information suppression of enzyme activity by small interfering RNA results in impairment of glucose-stimulated insulin secretion, attenuates glucose-induced increments in pyruvate cycling activity and in NADPH levels, and causes increases in lactate production Rattus norvegicus ? - ? 89 1.1.1.42 additional information NADP+-dependent isocitrate dehydrogenase may play an important role in regulating the apoptosis induced by heat shock Homo sapiens ? - ? 89 1.1.1.42 additional information NADP+-dependent isocitrate dehydrogenase may play an important role in regulating the apoptosis induced by TNF-alpha and anticancer drugs Homo sapiens ? - ? 89 1.1.1.42 additional information NADP+-dependent isocitrate dehydrogenase plays an important protective role in apoptosis of HL-60 cells induced by singlet oxygen Mus musculus ? - ? 89 1.1.1.42 additional information IDPm expression in HepG2 cells regulates ethanol-induced toxicity Mus musculus ? - ? 89 1.1.1.42 additional information does not use citrate as substrate Escherichia coli ? - ? 89 1.1.1.42 additional information the R132H mutant IDH1 directly converts 2-oxoglutarate to 2-hydroxyglutarate, that rapidly accumulates in the medium of cells expressing R132H mutant IDH1 Homo sapiens ? - ? 89 1.1.1.42 additional information Mycobacterium tuberculosis ICDH-1 also catalyzes the formation of 2-hydroxyglutarate Mycobacterium tuberculosis ? - ? 89 1.1.1.42 additional information the isocitrate molecule is located at the active site, and interacts with residues at the cleft between the large and small domain. The catalytic triad residues Lys191, Asp248 and Tyr144 are conserved and interact with isocitrate Thermus thermophilus ? - ? 89 1.1.1.42 additional information no activity Is detected when 0.12 mM NAD+, instead of NADP+, is added to the reaction mixture Psychromonas marina ? - - 89 1.1.1.42 additional information the enzyme is a substrate for Escherichia coli isocitrate dehydrogenase kinase/phosphatase Acinetobacter baumannii ? - - 89 1.1.1.42 additional information the enzyme is highly specific to NADP+ and cannot utilize NAD+ Methanococcoides methylutens ? - - 89 1.1.1.42 additional information the enzyme is highly specific to NADP+ and cannot utilize NAD+ Methanococcoides methylutens MM1 ? - - 89 1.1.1.42 additional information Mycobacterium tuberculosis ICDH-1 also catalyzes the formation of 2-hydroxyglutarate Mycobacterium tuberculosis H37Rv ? - ? 89 1.1.1.42 oxalosuccinate - Sus scrofa 2-oxoglutarate + CO2 - r 438419 1.1.1.42 oxalosuccinate - Thermus thermophilus 2-oxoglutarate + CO2 - r 438419 1.1.1.42 threo-Ds-isocitrate + NADP+ - Mus musculus 2-oxoglutarate + CO2 + NADPH - ? 374721