1.1.1.31 (R)-3-hydroxyisobutyrate + NAD+ - Oryctolagus cuniculus methylmalonate semialdehyde + NADH + H+ - r 453820 1.1.1.31 (R)-3-hydroxyisobutyrate + NAD+ 2% activity compared to (S)-3-hydroxyisobutyrate Arabidopsis thaliana 2-methyl-3-oxopropanoate + NADH + H+ - ? 458779 1.1.1.31 (R)-3-hydroxyisobutyrate + NAD+ substrate with much less efficiency compared to (S)-3-hydroxyisobutyrate Mycobacterium tuberculosis 2-methyl-3-oxopropanoate + NADH + H+ - ? 458779 1.1.1.31 (R)-3-hydroxyisobutyrate + NAD+ substrate with much less efficiency compared to (S)-3-hydroxyisobutyrate Mycobacterium tuberculosis H37Rv 2-methyl-3-oxopropanoate + NADH + H+ - ? 458779 1.1.1.31 (S)-3-hydroxyisobutyrate + NAD+ - Arabidopsis thaliana 2-methyl-3-oxopropanoate + NADH + H+ - ? 436673 1.1.1.31 (S)-3-hydroxyisobutyrate + NAD+ the enzyme is very specific to (S)-3-hydroxyisobutyrate Bacillus cereus 2-methyl-3-oxopropanoate + NADH + H+ - ? 436673 1.1.1.31 (S)-3-hydroxyisobutyrate + NAD+ the enzyme is very specific to (S)-3-hydroxyisobutyrate Pseudomonas denitrificans (nom. rej.) 2-methyl-3-oxopropanoate + NADH + H+ - ? 436673 1.1.1.31 (S)-3-hydroxyisobutyrate + NAD+ the enzyme is specific to (S)-3-hydroxyisobutyrate and prefers NAD+ as cofactor Mycobacterium tuberculosis 2-methyl-3-oxopropanoate + NADH + H+ - ? 436673 1.1.1.31 (S)-3-hydroxyisobutyrate + NAD+ the enzyme is specific to (S)-3-hydroxyisobutyrate Mycobacterium tuberculosis 2-methyl-3-oxopropanoate + NADH + H+ - ? 436673 1.1.1.31 (S)-3-hydroxyisobutyrate + NAD+ the enzyme is specific to (S)-3-hydroxyisobutyrate and prefers NAD+ as cofactor Mycobacterium tuberculosis H37Rv 2-methyl-3-oxopropanoate + NADH + H+ - ? 436673 1.1.1.31 (S)-3-hydroxyisobutyrate + NAD+ the enzyme is specific to (S)-3-hydroxyisobutyrate Mycobacterium tuberculosis H37Rv 2-methyl-3-oxopropanoate + NADH + H+ - ? 436673 1.1.1.31 (S)-3-hydroxyisobutyrate + NAD+ the enzyme is very specific to (S)-3-hydroxyisobutyrate Pseudomonas denitrificans (nom. rej.) ATCC 13867 2-methyl-3-oxopropanoate + NADH + H+ - ? 436673 1.1.1.31 (S)-3-hydroxyisobutyrate + NAD+ 100% activity Arabidopsis thaliana methylmalonate semialdehyde + NADH + H+ - ? 458792 1.1.1.31 (S)-3-hydroxyisobutyrate + NADP+ - Mycobacterium tuberculosis 2-methyl-3-oxopropanoate + NADPH + H+ - ? 458793 1.1.1.31 (S)-beta-hydroxyisobutyrate + NADP+ - Rattus norvegicus 2-methyl-3-oxopropanoate + NADPH + H+ - r 451034 1.1.1.31 (S)-methyl-3-hydroxy-2-methylpropionate + NAD+ 1.2% activity compared to (S)-3-hydroxyisobutyrate Arabidopsis thaliana ? - ? 458798 1.1.1.31 2-methyl-D,L-serine + NAD+ - Pseudomonas putida ? - ? 188042 1.1.1.31 2-methyl-D,L-serine + NAD+ - Pseudomonas putida E23 ? - ? 188042 1.1.1.31 3-hydroxy-2-methylpropanoate + NAD+ - Homo sapiens 2-methyl-3-oxopropanoate + NADH + H+ - ? 413200 1.1.1.31 3-hydroxy-2-methylpropanoate + NAD+ - Bacillus cereus 2-methyl-3-oxopropanoate + NADH + H+ - r 413200 1.1.1.31 3-hydroxy-2-methylpropanoate + NAD+ - Arabidopsis thaliana 2-methyl-3-oxopropanoate + NADH + H+ - ? 413200 1.1.1.31 3-hydroxy-2-methylpropanoate + NAD+ - Bacillus cereus ATCC 14579 2-methyl-3-oxopropanoate + NADH + H+ - r 413200 1.1.1.31 3-hydroxy-2-methylpropanoate + NADP+ - Bacillus cereus 2-methyl-3-oxopropanoate + NADPH + H+ - r 413201 1.1.1.31 3-hydroxy-2-methylpropanoate + NADP+ - Bacillus cereus ATCC 14579 2-methyl-3-oxopropanoate + NADPH + H+ - r 413201 1.1.1.31 3-hydroxyisobutyrate + NAD+ - Bacillus cereus methylmalonate semialdehyde + NADH + H+ - r 414040 1.1.1.31 3-hydroxyisobutyric acid + NAD+ - Homo sapiens methylmalonic semialdehyde + NADH - r 383832 1.1.1.31 3-hydroxypropanoate + NAD(P)+ is more active with NADP+ than NAD+ Bacillus cereus ? - ? 403735 1.1.1.31 3-hydroxypropanoate + NAD(P)+ is more active with NADP+ than NAD+ Bacillus cereus ATCC 14579 ? - ? 403735 1.1.1.31 3-hydroxypropanoate + NAD+ - Oryctolagus cuniculus ? - ? 188040 1.1.1.31 3-hydroxypropanoate + NAD+ - Pseudomonas putida ? - ? 188040 1.1.1.31 3-hydroxypropanoate + NAD+ - Pseudomonas putida E23 ? - ? 188040 1.1.1.31 3-hydroxypropanoate + NAD+ - Bacillus cereus malonate semialdehyde + NADH + H+ - r 414045 1.1.1.31 3-hydroxypropanoate + NADP+ - Bacillus cereus malonate semialdehyde + NADPH + H+ - r 414047 1.1.1.31 3-hydroxypropanoic acid + NAD+ substrate with much less efficiency compared to (S)-3-hydroxyisobutyrate Mycobacterium tuberculosis 3-oxopropanoate + NADH + H+ - ? 459032 1.1.1.31 3-hydroxypropionate + NAD+ - Bacillus cereus 3-oxopropanoate + NADH + H+ - ? 436872 1.1.1.31 3-hydroxypropionate + NAD+ 13% activity compared to (S)-3-hydroxyisobutyrate Arabidopsis thaliana malonate semialdehyde + NADH + H+ - ? 459033 1.1.1.31 3-hydroxypropionic acid + NAD+ - Pseudomonas denitrificans (nom. rej.) malonate semialdehyde + NADH + H+ - r 436873 1.1.1.31 3-hydroxypropionic acid + NAD+ - Pseudomonas denitrificans (nom. rej.) ATCC 13867 malonate semialdehyde + NADH + H+ - r 436873 1.1.1.31 D,L-3-hydroxyisobutyrate + NAD+ - Pseudomonas aeruginosa L-2-methyl-3-oxopropionate + NADH - ? 188036 1.1.1.31 D,L-3-hydroxyisobutyrate + NAD+ - Pseudomonas dacunhae L-2-methyl-3-oxopropionate + NADH - ? 188036 1.1.1.31 D,L-3-hydroxyisobutyrate + NAD+ - Galactomyces reessii L-2-methyl-3-oxopropionate + NADH - ? 188036 1.1.1.31 D,L-3-hydroxyisobutyrate + NAD+ - Nocardia lyena L-2-methyl-3-oxopropionate + NADH - ? 188036 1.1.1.31 D,L-3-hydroxyisobutyrate + NAD+ strain IFO 1542 Diutina rugosa L-2-methyl-3-oxopropionate + NADH - ? 188036 1.1.1.31 D,L-3-hydroxyisobutyrate + NAD+ - Candida parapsilosis D-2-methyl-3-oxopropionate + NADH - ? 188037 1.1.1.31 D,L-3-hydroxyisobutyrate + NAD+ strain IFO 0750 Diutina rugosa D-2-methyl-3-oxopropionate + NADH - ? 188037 1.1.1.31 D,L-3-hydroxyisobutyrate + NAD+ - Cereibacter sphaeroides 2-methyl-3-oxopropionate + NADH - ? 188038 1.1.1.31 D-3-hydroxyisobutyrate + NAD+ - Diutina rugosa (S)-methylmalonate semialdehyde + NADH + H+ - r 451035 1.1.1.31 D-3-hydroxyisobutyrate + NAD+ - Diutina rugosa methylmalonate semialdehyde + NADH + H+ - r 451037 1.1.1.31 DL-3-hydroxyisobutyrate + NAD+ - Sus scrofa methylmalonate semialdehyde + NADH + H+ - r 451039 1.1.1.31 L-3-hydroxyisobutyrate + NAD+ - Rattus norvegicus L-2-methyl-3-oxopropionate + NADH - ? 188039 1.1.1.31 L-3-hydroxyisobutyrate + NAD+ - Sus scrofa L-2-methyl-3-oxopropionate + NADH - ? 188039 1.1.1.31 L-3-hydroxyisobutyrate + NAD+ - Bos taurus L-2-methyl-3-oxopropionate + NADH - ? 188039 1.1.1.31 L-3-hydroxyisobutyrate + NAD+ - Oryctolagus cuniculus L-2-methyl-3-oxopropionate + NADH - ? 188039 1.1.1.31 L-3-hydroxyisobutyrate + NAD+ - Oryctolagus cuniculus L-2-methyl-3-oxopropionate + NADH - r 188039 1.1.1.31 L-3-hydroxyisobutyrate + NAD+ - Pseudomonas putida L-2-methyl-3-oxopropionate + NADH - ? 188039 1.1.1.31 L-3-hydroxyisobutyrate + NAD+ - Micrococcus luteus L-2-methyl-3-oxopropionate + NADH - ? 188039 1.1.1.31 L-3-hydroxyisobutyrate + NAD+ - Rhodotorula mucilaginosa L-2-methyl-3-oxopropionate + NADH - ? 188039 1.1.1.31 L-3-hydroxyisobutyrate + NAD+ - Micrococcus flavus L-2-methyl-3-oxopropionate + NADH - ? 188039 1.1.1.31 L-3-hydroxyisobutyrate + NAD+ - Corynebacterium hydrocarbons L-2-methyl-3-oxopropionate + NADH - ? 188039 1.1.1.31 L-3-hydroxyisobutyrate + NAD+ - Tsukamurella paurometabola L-2-methyl-3-oxopropionate + NADH - ? 188039 1.1.1.31 L-3-hydroxyisobutyrate + NAD+ - Aciculoconidium aculeatum L-2-methyl-3-oxopropionate + NADH - ? 188039 1.1.1.31 L-3-hydroxyisobutyrate + NAD+ - Pseudomonas putida E23 L-2-methyl-3-oxopropionate + NADH - ? 188039 1.1.1.31 L-3-hydroxyisobutyrate + NAD+ high stereospecificity Pseudomonas putida 3-oxoisobutyrate + NADH - r 373527 1.1.1.31 L-3-hydroxyisobutyrate + NAD+ high stereospecificity Pseudomonas putida E23 3-oxoisobutyrate + NADH - r 373527 1.1.1.31 L-3-hydroxyisobutyrate + NAD+ - Diutina rugosa (S)-methylmalonate semialdehyde + NADH + H+ - r 451036 1.1.1.31 L-3-hydroxyisobutyrate + NAD+ - Diutina rugosa methylmalonate semialdehyde + NADH + H+ - r 451038 1.1.1.31 L-serine + NAD+ - Pseudomonas putida ? - ? 188041 1.1.1.31 L-serine + NAD+ substrate with much less efficiency compared to (S)-3-hydroxyisobutyrate Mycobacterium tuberculosis ? - ? 188041 1.1.1.31 L-serine + NAD+ substrate with much less efficiency compared to (S)-3-hydroxyisobutyrate Mycobacterium tuberculosis H37Rv ? - ? 188041 1.1.1.31 L-serine + NAD+ - Pseudomonas putida E23 ? - ? 188041 1.1.1.31 L-serine + NAD+ - Bacillus cereus 3-oxo-L-alanine + NADH + H+ - r 437649 1.1.1.31 L-serine + NAD+ - Pseudomonas denitrificans (nom. rej.) 3-oxo-L-alanine + NADH + H+ - r 437649 1.1.1.31 L-serine + NAD+ - Pseudomonas denitrificans (nom. rej.) ATCC 13867 3-oxo-L-alanine + NADH + H+ - r 437649 1.1.1.31 methyl (2S)-3-hydroxy-2-methylpropanoate + NAD+ - Pseudomonas denitrificans (nom. rej.) methyl (2S)-2-methyl-3-oxopropanoate + NADH + H+ - r 437692 1.1.1.31 methyl (2S)-3-hydroxy-2-methylpropanoate + NAD+ - Pseudomonas denitrificans (nom. rej.) ATCC 13867 methyl (2S)-2-methyl-3-oxopropanoate + NADH + H+ - r 437692 1.1.1.31 methyl DL-serine + NAD+ - Pseudomonas denitrificans (nom. rej.) methyl 3-oxo-DL-alanine + NADH + H+ - r 437699 1.1.1.31 methyl DL-serine + NAD+ - Pseudomonas denitrificans (nom. rej.) ATCC 13867 methyl 3-oxo-DL-alanine + NADH + H+ - r 437699 1.1.1.31 additional information the enzyme catalyzes the rate-limiting step in the irreversible degradative pathway for the carbon skeleton of valine and the other branched-chain amino acids Rattus norvegicus ? - ? 89 1.1.1.31 additional information MmsB from Bacillus cereus exhibits 3-hydroxyisobutyrate dehydrogenase as well as 3-hydroxypropionate dehydrogenase activity, EC 1.1.1.59 Bacillus cereus ? - ? 89 1.1.1.31 additional information wide substrate specificity of the 3-HIBADH Bacillus cereus ? - ? 89 1.1.1.31 additional information no activity with (R)-3-hydroxyisobutyrate Pseudomonas denitrificans (nom. rej.) ? - ? 89 1.1.1.31 additional information no activity with NADP+, DL-glycerate, (R)-3-hydroxyisobutyrate, L-threonine, D-threonine, and D-serine Bacillus cereus ? - ? 89 1.1.1.31 additional information no activity with (R)-methyl-3-hydroxy-2-methylpropionate, glycerate, serine, beta-hydroxypyruvate, threonine, 2-hydroxybutyrate, malate, glycolate, beta-hydroxybutyrate, lactate, and 6-phosphogluconate. NADP+ is not a suitable cofactor. Also, FAD, oxidized cytochrome c, as well as the artificial electron acceptors phenazine methosulfate and dichlorophenol indophenol cannot be reduced by the enzyme Arabidopsis thaliana ? - - 89 1.1.1.31 additional information no activity with D-serine, (S)-3-hydroxyisobutyrate methyl ester, (R)-3-hydroxyisobutyrate methyl ester, L-threonine and D-threonine Mycobacterium tuberculosis ? - - 89 1.1.1.31 additional information MmsB from Bacillus cereus exhibits 3-hydroxyisobutyrate dehydrogenase as well as 3-hydroxypropionate dehydrogenase activity, EC 1.1.1.59 Bacillus cereus ATCC 14579 ? - ? 89 1.1.1.31 additional information wide substrate specificity of the 3-HIBADH Bacillus cereus ATCC 14579 ? - ? 89 1.1.1.31 additional information no activity with D-serine, (S)-3-hydroxyisobutyrate methyl ester, (R)-3-hydroxyisobutyrate methyl ester, L-threonine and D-threonine Mycobacterium tuberculosis H37Rv ? - - 89 1.1.1.31 additional information no activity with (R)-3-hydroxyisobutyrate Pseudomonas denitrificans (nom. rej.) ATCC 13867 ? - ? 89