1.1.1.176 12-ketochenodeoxycholic acid + NADH - Brevibacterium fuscum cholic acid + NAD+ - r 370087 1.1.1.176 12-oxo-cholic acid + NAD(P)H + H+ 4.1% of the activity compared to the substrate 12-oxo-deoxycholic acid Eggerthella sp. CAG:298 cholic acid + NAD(P)+ - r 458884 1.1.1.176 12-oxo-deoxycholic acid + NAD(P)H + H+ - Eggerthella sp. CAG:298 deoxycholic acid + NAD(P)+ - r 458885 1.1.1.176 12-oxochenodeoxycholic acid + NADP+ - Clostridium sp. (3alpha,5beta,7alpha,12alpha)-3,7,12-trihydroxycholan-24-oic acid + NADPH + H+ - ? 230322 1.1.1.176 12-oxochenodeoxycholic acid + NADPH + H+ 3.65% activity compared to 12-oxolithocholic acid Peptacetobacter hiranonis 7-oxodeoxycholic acid + NADP+ - ? 458886 1.1.1.176 12-oxochenodeoxycholic acid + NADPH + H+ 4.16% activity compared to 12-oxolithocholic acid [Clostridium] hylemonae 7-oxodeoxycholic acid + NADP+ - ? 458886 1.1.1.176 12-oxochenodeoxycholic acid + NADPH + H+ 9.55% activity compared to 12-oxolithocholic acid [Clostridium] scindens 7-oxodeoxycholic acid + NADP+ - ? 458886 1.1.1.176 12-oxochenodeoxycholic acid + NADPH + H+ 9.55% activity compared to 12-oxolithocholic acid [Clostridium] scindens ATCC 35704 7-oxodeoxycholic acid + NADP+ - ? 458886 1.1.1.176 12-oxochenodeoxycholic acid + NADPH + H+ 3.65% activity compared to 12-oxolithocholic acid Peptacetobacter hiranonis DSM 13275 7-oxodeoxycholic acid + NADP+ - ? 458886 1.1.1.176 12-oxochenodeoxycholic acid + NADPH + H+ 4.16% activity compared to 12-oxolithocholic acid [Clostridium] hylemonae DSM 15053 7-oxodeoxycholic acid + NADP+ - ? 458886 1.1.1.176 12-oxolithocholic acid + NADH + H+ - Eggerthella lenta deoxycholic acid + NAD+ - r 458415 1.1.1.176 12-oxolithocholic acid + NADH + H+ 19.6% activity compared to deoxycholic acid Eggerthella lenta deoxycholic acid + NAD+ - ? 458415 1.1.1.176 12-oxolithocholic acid + NADH + H+ - Eggerthella lenta C592 deoxycholic acid + NAD+ - r 458415 1.1.1.176 12-oxolithocholic acid + NADH + H+ 19.6% activity compared to deoxycholic acid Eggerthella lenta C592 deoxycholic acid + NAD+ - ? 458415 1.1.1.176 12-oxolithocholic acid + NADH + H+ - Eggerthella lenta DSM 2243T deoxycholic acid + NAD+ - r 458415 1.1.1.176 12-oxolithocholic acid + NADH + H+ 19.6% activity compared to deoxycholic acid Eggerthella lenta DSM 2243T deoxycholic acid + NAD+ - ? 458415 1.1.1.176 12-oxolithocholic acid + NADPH + H+ - Eggerthella lenta deoxycholic acid + NADP+ - r 458416 1.1.1.176 12-oxolithocholic acid + NADPH + H+ 100% activity [Clostridium] scindens deoxycholic acid + NADP+ - ? 458416 1.1.1.176 12-oxolithocholic acid + NADPH + H+ 100% activity Peptacetobacter hiranonis deoxycholic acid + NADP+ - ? 458416 1.1.1.176 12-oxolithocholic acid + NADPH + H+ 100% activity [Clostridium] hylemonae deoxycholic acid + NADP+ - ? 458416 1.1.1.176 12-oxolithocholic acid + NADPH + H+ 100% activity [Clostridium] scindens ATCC 35704 deoxycholic acid + NADP+ - ? 458416 1.1.1.176 12-oxolithocholic acid + NADPH + H+ 100% activity Peptacetobacter hiranonis DSM 13275 deoxycholic acid + NADP+ - ? 458416 1.1.1.176 12-oxolithocholic acid + NADPH + H+ 100% activity [Clostridium] hylemonae DSM 15053 deoxycholic acid + NADP+ - ? 458416 1.1.1.176 3-oxocholic acid + NADPH + H+ - [Clostridium] scindens cholic acid + NADP+ - r 459045 1.1.1.176 3-oxocholic acid + NADPH + H+ - Peptacetobacter hiranonis cholic acid + NADP+ - r 459045 1.1.1.176 3-oxocholic acid + NADPH + H+ - [Clostridium] hylemonae cholic acid + NADP+ - r 459045 1.1.1.176 3-oxocholic acid + NADPH + H+ - [Clostridium] scindens ATCC 35704 cholic acid + NADP+ - r 459045 1.1.1.176 3-oxocholic acid + NADPH + H+ - Peptacetobacter hiranonis DSM 13275 cholic acid + NADP+ - r 459045 1.1.1.176 3-oxocholic acid + NADPH + H+ - [Clostridium] hylemonae DSM 15053 cholic acid + NADP+ - r 459045 1.1.1.176 3alpha,7alpha,12alpha-trihydroxy-5beta-cholanoyl methyl ester + NADP+ - Eggerthella lenta 3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanoyl methyl ester + NADPH + H+ - r 450980 1.1.1.176 5beta-cholanic acid-3,12-dione + NADPH + H+ - [Clostridium] scindens 3-oxodeoxycholic acid + NADP+ - ? 459108 1.1.1.176 5beta-cholanic acid-3,12-dione + NADPH + H+ - Peptacetobacter hiranonis 3-oxodeoxycholic acid + NADP+ - ? 459108 1.1.1.176 5beta-cholanic acid-3,12-dione + NADPH + H+ - [Clostridium] hylemonae 3-oxodeoxycholic acid + NADP+ - ? 459108 1.1.1.176 5beta-cholanic acid-3,12-dione + NADPH + H+ - [Clostridium] scindens ATCC 35704 3-oxodeoxycholic acid + NADP+ - ? 459108 1.1.1.176 5beta-cholanic acid-3,12-dione + NADPH + H+ - Peptacetobacter hiranonis DSM 13275 3-oxodeoxycholic acid + NADP+ - ? 459108 1.1.1.176 5beta-cholanic acid-3,12-dione + NADPH + H+ - [Clostridium] hylemonae DSM 15053 3-oxodeoxycholic acid + NADP+ - ? 459108 1.1.1.176 5beta-cholanic acid-3,7,12-trione + NADPH + H+ - [Clostridium] scindens cholic acid + deoxycholic acid + 3-oxodeoxycholic acid + NADP+ - ? 459109 1.1.1.176 5beta-cholanic acid-3,7,12-trione + NADPH + H+ - Peptacetobacter hiranonis cholic acid + deoxycholic acid + 3-oxodeoxycholic acid + NADP+ - ? 459109 1.1.1.176 5beta-cholanic acid-3,7,12-trione + NADPH + H+ - [Clostridium] hylemonae cholic acid + deoxycholic acid + 3-oxodeoxycholic acid + NADP+ - ? 459109 1.1.1.176 5beta-cholanic acid-3alpha-ol-7,12-dione + NADPH + H+ - [Clostridium] scindens cholic acid + deoxycholic acid + 3-oxodeoxycholic acid + NADP+ - ? 459110 1.1.1.176 5beta-cholanic acid-3alpha-ol-7,12-dione + NADPH + H+ - Peptacetobacter hiranonis cholic acid + deoxycholic acid + 3-oxodeoxycholic acid + NADP+ - ? 459110 1.1.1.176 5beta-cholanic acid-3alpha-ol-7,12-dione + NADPH + H+ - [Clostridium] hylemonae cholic acid + deoxycholic acid + 3-oxodeoxycholic acid + NADP+ - ? 459110 1.1.1.176 7-oxocholic acid + NADPH + H+ - [Clostridium] scindens deoxycholic acid + NADP+ - r 459127 1.1.1.176 7-oxocholic acid + NADPH + H+ - Peptacetobacter hiranonis deoxycholic acid + NADP+ - r 459127 1.1.1.176 7alpha,12alpha-dihydroxy-5beta-cholanoate + NAD+ - Eggerthella lenta 7alpha-hydroxy-12-oxo-5beta-cholanoate + NADH - ? 230321 1.1.1.176 allocholate + NAD+ - Eggerthella lenta 3alpha,7alpha-dihydroxy-12-oxo-5alpha-cholan-24-oic acid + NADH - ? 230319 1.1.1.176 allodeoxycholate + NAD+ - Eggerthella lenta 3alpha-hydroxy-12-oxo-5alpha-cholan-24-oic acid + NADH - ? 230320 1.1.1.176 cholate + NAD+ - Clostridium perfringens 3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanate + NADH + H+ - ? 438283 1.1.1.176 cholate + NAD+ - Eggerthella lenta 3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanate + NADH + H+ - ? 438283 1.1.1.176 cholate + NADP+ - Clostridium sp. 3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanate + NADPH + H+ - ? 438284 1.1.1.176 cholate + NADP+ - [Clostridium] leptum 3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanate + NADPH + H+ - ? 438284 1.1.1.176 cholate + NADP+ conversion of CA into 12-oxoursodeoxycholic acid Clostridium sp. 3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanate + NADPH + H+ - ? 438284 1.1.1.176 cholate + NADP+ conversion of CA into 12-oxoursodeoxycholic acid Clostridium perfringens 3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanate + NADPH + H+ - ? 438284 1.1.1.176 cholate + NADP+ conversion of CA into 12-oxoursodeoxycholic acid Eggerthella lenta 3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanate + NADPH + H+ - ? 438284 1.1.1.176 cholate + NADP+ conversion of CA into 12-oxoursodeoxycholic acid [Clostridium] leptum 3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanate + NADPH + H+ - ? 438284 1.1.1.176 cholate + NADP+ - Clostridium sp. ATCC 29733 3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanate + NADPH + H+ - ? 438284 1.1.1.176 cholate + NADP+ conversion of CA into 12-oxoursodeoxycholic acid Clostridium sp. ATCC 29733 3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanate + NADPH + H+ - ? 438284 1.1.1.176 cholic acid + NAD(P)+ - Clostridium perfringens 3alpha,7alpha-dihydroxy-12-oxo-5beta-cholan-24-oic acid + NAD(P)H - ? 230313 1.1.1.176 cholic acid + NAD(P)+ - Brevibacterium fuscum 3alpha,7alpha-dihydroxy-12-oxo-5beta-cholan-24-oic acid + NAD(P)H - ? 230313 1.1.1.176 cholic acid + NAD(P)+ - Brevibacterium fuscum 3alpha,7alpha-dihydroxy-12-oxo-5beta-cholan-24-oic acid + NAD(P)H - r 230313 1.1.1.176 cholic acid + NAD(P)+ - Eggerthella lenta 3alpha,7alpha-dihydroxy-12-oxo-5beta-cholan-24-oic acid + NAD(P)H - ? 230313 1.1.1.176 cholic acid + NAD(P)+ - [Clostridium] leptum 3alpha,7alpha-dihydroxy-12-oxo-5beta-cholan-24-oic acid + NAD(P)H - ? 230313 1.1.1.176 cholic acid + NAD(P)+ hardly accepts N6-modified NADP+ derivative Clostridium sp. 3alpha,7alpha-dihydroxy-12-oxo-5beta-cholan-24-oic acid + NAD(P)H - ? 230313 1.1.1.176 cholic acid + NAD(P)+ hardly accepts N6-modified NADP+ derivative Clostridium sp. 3alpha,7alpha-dihydroxy-12-oxo-5beta-cholan-24-oic acid + NAD(P)H - r 230313 1.1.1.176 cholic acid + NAD(P)+ i.e. 3alpha,7alpha,12alpha-trihydroxy-5beta-cholan-24-oic acid Clostridium sp. 3alpha,7alpha-dihydroxy-12-oxo-5beta-cholan-24-oic acid + NAD(P)H - ? 230313 1.1.1.176 cholic acid + NAD(P)+ i.e. 3alpha,7alpha,12alpha-trihydroxy-5beta-cholan-24-oic acid Clostridium sp. 3alpha,7alpha-dihydroxy-12-oxo-5beta-cholan-24-oic acid + NAD(P)H - r 230313 1.1.1.176 cholic acid + NAD(P)+ 55.7% of the activity compared to the substrate 12-oxo-deoxycholic acid Eggerthella sp. CAG:298 12-oxocholic acid + NAD(P)H + H+ - r 461408 1.1.1.176 cholic acid + NAD+ 98.5% activity compared to deoxycholic acid Eggerthella lenta 3-oxocholic acid + NADH + H+ - r 437276 1.1.1.176 cholic acid + NAD+ 98.5% activity compared to deoxycholic acid Eggerthella lenta C592 3-oxocholic acid + NADH + H+ - r 437276 1.1.1.176 cholic acid + NAD+ 98.5% activity compared to deoxycholic acid Eggerthella lenta DSM 2243T 3-oxocholic acid + NADH + H+ - r 437276 1.1.1.176 cholic acid + NADP+ the assay is performed using an NADP+-regenerating system with acetone, NADPH, 2-propanol and alcohol dehydrogenase from Lactobacillus brevis or Thermoanaerobium brockii, overview Clostridium sp. 12-ketochenodeoxycholic acid + NADPH + H+ - r 384448 1.1.1.176 cholic acid + NADP+ 54.44% activity compared to deoxycholic acid [Clostridium] scindens 3-oxocholic acid + NADPH + H+ - r 459517 1.1.1.176 cholic acid + NADP+ 80.51% activity compared to deoxycholic acid [Clostridium] hylemonae 3-oxocholic acid + NADPH + H+ - r 459517 1.1.1.176 cholic acid + NADP+ 82.32% activity compared to deoxycholic acid Peptacetobacter hiranonis 3-oxocholic acid + NADPH + H+ - r 459517 1.1.1.176 deoxycholate + NADP+ 3alpha,12alpha-dihydroxy-5beta-cholan-24-oic acid Curvularia lunata 12-oxo-lithocholic acid + NADPH + H+ i.e. 3alpha-hydroxy-12-oxo-5beta-cholan-24-oic acid ? 439486 1.1.1.176 deoxycholate + NADP+ 3alpha,12alpha-dihydroxy-5beta-cholan-24-oic acid Curvularia lunata VKM F-644 12-oxo-lithocholic acid + NADPH + H+ i.e. 3alpha-hydroxy-12-oxo-5beta-cholan-24-oic acid ? 439486 1.1.1.176 deoxycholic acid + NAD(P)+ i.e. 3alpha,12alpha-dihydroxy-5beta-cholan-24-oic acid Clostridium sp. 3alpha-hydroxy-12-oxo-5beta-cholan-24-oic acid + NAD(P)H + H+ - ? 230314 1.1.1.176 deoxycholic acid + NAD(P)+ i.e. 3alpha,12alpha-dihydroxy-5beta-cholan-24-oic acid Clostridium perfringens 3alpha-hydroxy-12-oxo-5beta-cholan-24-oic acid + NAD(P)H + H+ - ? 230314 1.1.1.176 deoxycholic acid + NAD(P)+ i.e. 3alpha,12alpha-dihydroxy-5beta-cholan-24-oic acid Brevibacterium fuscum 3alpha-hydroxy-12-oxo-5beta-cholan-24-oic acid + NAD(P)H + H+ - ? 230314 1.1.1.176 deoxycholic acid + NAD(P)+ i.e. 3alpha,12alpha-dihydroxy-5beta-cholan-24-oic acid Brevibacterium fuscum 3alpha-hydroxy-12-oxo-5beta-cholan-24-oic acid + NAD(P)H + H+ - r 230314 1.1.1.176 deoxycholic acid + NAD(P)+ i.e. 3alpha,12alpha-dihydroxy-5beta-cholan-24-oic acid Eggerthella lenta 3alpha-hydroxy-12-oxo-5beta-cholan-24-oic acid + NAD(P)H + H+ - ? 230314 1.1.1.176 deoxycholic acid + NAD(P)+ i.e. 3alpha,12alpha-dihydroxy-5beta-cholan-24-oic acid [Clostridium] leptum 3alpha-hydroxy-12-oxo-5beta-cholan-24-oic acid + NAD(P)H + H+ - ? 230314 1.1.1.176 deoxycholic acid + NAD(P)+ 51.9% of the activity compared to the substrate 12-oxo-deoxycholic acid Eggerthella sp. CAG:298 12-oxo-deoxycholic acid + NAD(P)H + H+ - r 459609 1.1.1.176 deoxycholic acid + NAD+ - Acinetobacter lwoffii 3alpha-hydroxy-12-oxo-5beta-cholan-24-oic acid + NADH + H+ - ? 404978 1.1.1.176 deoxycholic acid + NAD+ 100% activity Eggerthella lenta 12-oxolithocholic acid + NADH + H+ - r 459608 1.1.1.176 deoxycholic acid + NADP+ 3.14% activity compared to NAD+ Eggerthella lenta 12-oxolithocholic acid + NADH + H+ - r 459610 1.1.1.176 deoxycholic acid + NADP+ 100% activity [Clostridium] scindens 7-oxocholic acid + NADPH + H+ - r 459613 1.1.1.176 deoxycholic acid + NADP+ 100% activity Peptacetobacter hiranonis 7-oxocholic acid + NADPH + H+ - r 459613 1.1.1.176 deoxycholic acid + NADP+ 100% activity [Clostridium] hylemonae 7-oxocholic acid + NADPH + H+ - ? 459613 1.1.1.176 glycocholic acid + NAD(P)+ 60.3% of the activity compared to the substrate 12-oxo-deoxycholic acid Eggerthella sp. CAG:298 ? + NAD(P)H + H+ - r 459688 1.1.1.176 glycocholic acid + NAD+ i.e. 3alpha,7alpha,12alpha-trihydroxy-5beta-cholanoyl glycine Clostridium perfringens 3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanoyl glycine + NADH - ? 230315 1.1.1.176 glycocholic acid + NAD+ i.e. 3alpha,7alpha,12alpha-trihydroxy-5beta-cholanoyl glycine Brevibacterium fuscum 3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanoyl glycine + NADH - ? 230315 1.1.1.176 glycocholic acid + NAD+ i.e. 3alpha,7alpha,12alpha-trihydroxy-5beta-cholanoyl glycine Eggerthella lenta 3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanoyl glycine + NADH - ? 230315 1.1.1.176 glycocholic acid + NAD+ i.e. 3alpha,7alpha,12alpha-trihydroxy-5beta-cholanoyl glycine [Clostridium] leptum 3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanoyl glycine + NADH - ? 230315 1.1.1.176 glycocholic acid + NAD+ 81.08% activity compared to deoxycholic acid Eggerthella lenta 3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanoyl glycine + NADH - r 230315 1.1.1.176 glycocholic acid + NAD+ 81.08% activity compared to deoxycholic acid Eggerthella lenta C592 3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanoyl glycine + NADH - r 230315 1.1.1.176 glycocholic acid + NAD+ 81.08% activity compared to deoxycholic acid Eggerthella lenta DSM 2243T 3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanoyl glycine + NADH - r 230315 1.1.1.176 glycodeoxycholic acid + NAD(P)+ 55.9% of the activity compared to the substrate 12-oxo-deoxycholic acid Eggerthella sp. CAG:298 ? + NAD(P)H + H+ - r 459689 1.1.1.176 glycodeoxycholic acid + NAD+ i.e. 3alpha,12alpha-dihydroxy-5beta-cholanoyl glycine Clostridium sp. 3alpha-hydroxy-12-oxo-5beta-cholanoyl glycine + NADH - ? 230317 1.1.1.176 glycodeoxycholic acid + NAD+ i.e. 3alpha,12alpha-dihydroxy-5beta-cholanoyl glycine Brevibacterium fuscum 3alpha-hydroxy-12-oxo-5beta-cholanoyl glycine + NADH - ? 230317 1.1.1.176 glycodeoxycholic acid + NAD+ i.e. 3alpha,12alpha-dihydroxy-5beta-cholanoyl glycine Eggerthella lenta 3alpha-hydroxy-12-oxo-5beta-cholanoyl glycine + NADH - ? 230317 1.1.1.176 glycodeoxycholic acid + NAD+ i.e. 3alpha,12alpha-dihydroxy-5beta-cholanoyl glycine [Clostridium] leptum 3alpha-hydroxy-12-oxo-5beta-cholanoyl glycine + NADH - ? 230317 1.1.1.176 glycodeoxycholic acid + NAD+ 97.8% activity compared to deoxycholic acid Eggerthella lenta ? + NADH + H+ - r 459712 1.1.1.176 glycodeoxycholic acid + NADP+ 24.17% activity compared to deoxycholic acid [Clostridium] hylemonae ? + NADPH + H+ - r 459713 1.1.1.176 glycodeoxycholic acid + NADP+ 25.85% activity compared to deoxycholic acid Peptacetobacter hiranonis ? + NADPH + H+ - r 459713 1.1.1.176 glycodeoxycholic acid + NADP+ 6.36% activity compared to deoxycholic acid [Clostridium] scindens ? + NADPH + H+ - r 459713 1.1.1.176 additional information multistep conversion of cholic acid into ursodeoxycholic acid. Enzyme sources are 7alpha-HSDH from Clostridium absonum (crude extract), 12alpha-HSDH from Clostridium group P, 7beta-HSDH from Clostridium absonum Clostridium sp. ? - ? 89 1.1.1.176 additional information reaction product analysis confirmed by MS and NMR analyses Curvularia lunata ? - ? 89 1.1.1.176 additional information no activity with chenodeoxycholic acid, glycochenodeoxycholic acid, taurochenodeoxycholic acid, 12-oxochenodeoxycholic acid, 3-oxodeoxycholic acid, and 3-oxocholic acid Eggerthella lenta ? - - 89 1.1.1.176 additional information no detection of the formation of 5beta-cholanic acid-3alpha-ol-12-one when cholic acid or its C-3'- or C-7'-oxo derivative is the substrate. No detection of oxidation of the 12alpha-hydroxyl group when deoxycholic acid is the substrate. No activity with 5beta-cholanic acid-12alpha-ol-3-one. No activity with NADH Peptacetobacter hiranonis ? - - 89 1.1.1.176 additional information no detection of the formation of 5beta-cholanic acid-3alpha-ol-12-one when cholic acid or its C-3- or C-7-oxo derivative is the substrate. No detection of oxidation of the 12alpha-hydroxyl group when deoxycholic acid is the substrate. No activity with 5beta-cholanic acid-12alpha-ol-3-one and 7-oxocholic acid. No activity with NADH [Clostridium] hylemonae ? - - 89 1.1.1.176 additional information no detection of the formation of 5beta-cholanic acid-3alpha-ol-12-one when cholic acid or its C-3- or C-7-oxo derivative is the substrate. No detection of oxidation of the 12alpha-hydroxyl group when deoxycholic acid is the substrate. No activity with 5beta-cholanic acid-12alpha-ol-3-one. No activity with NADH [Clostridium] scindens ? - - 89 1.1.1.176 additional information no activity with chenodeoxycholic acid, glycochenodeoxycholic acid, taurochenodeoxycholic acid, 12-oxochenodeoxycholic acid, 3-oxodeoxycholic acid, and 3-oxocholic acid Eggerthella lenta C592 ? - - 89 1.1.1.176 additional information multistep conversion of cholic acid into ursodeoxycholic acid. Enzyme sources are 7alpha-HSDH from Clostridium absonum (crude extract), 12alpha-HSDH from Clostridium group P, 7beta-HSDH from Clostridium absonum Clostridium sp. ATCC 29733 ? - ? 89 1.1.1.176 additional information no detection of the formation of 5beta-cholanic acid-3alpha-ol-12-one when cholic acid or its C-3- or C-7-oxo derivative is the substrate. No detection of oxidation of the 12alpha-hydroxyl group when deoxycholic acid is the substrate. No activity with 5beta-cholanic acid-12alpha-ol-3-one. No activity with NADH [Clostridium] scindens ATCC 35704 ? - - 89 1.1.1.176 additional information reaction product analysis confirmed by MS and NMR analyses Curvularia lunata VKM F-644 ? - ? 89 1.1.1.176 additional information no detection of the formation of 5beta-cholanic acid-3alpha-ol-12-one when cholic acid or its C-3'- or C-7'-oxo derivative is the substrate. No detection of oxidation of the 12alpha-hydroxyl group when deoxycholic acid is the substrate. No activity with 5beta-cholanic acid-12alpha-ol-3-one. No activity with NADH Peptacetobacter hiranonis DSM 13275 ? - - 89 1.1.1.176 additional information no detection of the formation of 5beta-cholanic acid-3alpha-ol-12-one when cholic acid or its C-3- or C-7-oxo derivative is the substrate. No detection of oxidation of the 12alpha-hydroxyl group when deoxycholic acid is the substrate. No activity with 5beta-cholanic acid-12alpha-ol-3-one and 7-oxocholic acid. No activity with NADH [Clostridium] hylemonae DSM 15053 ? - - 89 1.1.1.176 additional information no activity with chenodeoxycholic acid, glycochenodeoxycholic acid, taurochenodeoxycholic acid, 12-oxochenodeoxycholic acid, 3-oxodeoxycholic acid, and 3-oxocholic acid Eggerthella lenta DSM 2243T ? - - 89 1.1.1.176 taurocholic acid + NAD(P)+ - [Clostridium] leptum 3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanoyl taurine + NAD(P)H - ? 230316 1.1.1.176 taurocholic acid + NAD(P)+ i.e. 3alpha,7alpha,12alpha-trihydroxy-5beta-cholanoyl taurine Clostridium perfringens 3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanoyl taurine + NAD(P)H - ? 230316 1.1.1.176 taurocholic acid + NAD(P)+ i.e. 3alpha,7alpha,12alpha-trihydroxy-5beta-cholanoyl taurine Brevibacterium fuscum 3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanoyl taurine + NAD(P)H - ? 230316 1.1.1.176 taurocholic acid + NAD(P)+ i.e. 3alpha,7alpha,12alpha-trihydroxy-5beta-cholanoyl taurine Eggerthella lenta 3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanoyl taurine + NAD(P)H - ? 230316 1.1.1.176 taurocholic acid + NAD(P)+ 53.8% of the activity compared to the substrate 12-oxo-deoxycholic acid Eggerthella sp. CAG:298 2-[(3alpha,7alpha-dihydroxy-12,24-dioxo-5beta-cholan-24-yl)amino]ethane-1-sulfonic acid + NAD(P)H + H+ - r 461407 1.1.1.176 taurocholic acid + NAD+ 98.95% activity compared to deoxycholic acid Eggerthella lenta ? + NADH + H+ - r 459903 1.1.1.176 taurodeoxychloic acid + NAD(P)+ 58.9% of the activity compared to the substrate 12-oxo-deoxycholic acid Eggerthella sp. CAG:298 ? + NAD(P)H + H+ - r 461404 1.1.1.176 taurodeoxycholic acid + NAD+ - Brevibacterium fuscum 3alpha-hydroxy-12-oxo-5beta-cholanoyl taurine + NADH - ? 230318 1.1.1.176 taurodeoxycholic acid + NAD+ - [Clostridium] leptum 3alpha-hydroxy-12-oxo-5beta-cholanoyl taurine + NADH - ? 230318 1.1.1.176 taurodeoxycholic acid + NAD+ 90.84% activity compared to deoxycholic acid Eggerthella lenta ? + NADH + H+ - r 459904 1.1.1.176 taurodeoxycholic acid + NADP+ 40.33% activity compared to deoxycholic acid Peptacetobacter hiranonis ? + NADPH + H+ - r 459905 1.1.1.176 taurodeoxycholic acid + NADP+ 45.08% activity compared to deoxycholic acid [Clostridium] hylemonae ? + NADPH + H+ - r 459905 1.1.1.176 taurodeoxycholic acid + NADP+ 6.05% activity compared to deoxycholic acid [Clostridium] scindens ? + NADPH + H+ - r 459905