4.2.1.34 (2S,3S)-tartrate - Escherichia coli oxaloacetate + H2O - ? 444501 4.2.1.34 (2S,3S)-tartrate - Escherichia coli ATCC 700728 / O157:H7 oxaloacetate + H2O - ? 444501 4.2.1.34 (2S,3S)-tartrate - Escherichia coli W3110 / K-12 oxaloacetate + H2O - ? 444501 4.2.1.34 (L)-citramalate - Clostridium tetanomorphum mesaconate + H2O - ? 407628 4.2.1.34 (L)-citramalate - Aerobic bacterium mesaconate + H2O - ? 407628 4.2.1.34 (L)-citramalate enzyme is specific to (L)-citramalate, the rate of dehydration of (D)-citramalate is below 10% of that for the (L) isomer Rhodospirillum rubrum mesaconate + H2O - r 407628 4.2.1.34 (L)-citramalate enzyme is specific to (L)-citramalate, the rate of dehydration of (D)-citramalate is below 10% of that for the (L) isomer Rhodospirillum rubrum 1R mesaconate + H2O - r 407628 4.2.1.34 (S)-citramalate - Escherichia coli mesaconate + H2O - r 444567 4.2.1.34 (S)-citramalate - Paraburkholderia xenovorans mesaconate + H2O - r 444567 4.2.1.34 (S)-citramalate the catalytic efficiency of FumA with (S)-citramalate/mesaconate is about 4% of that with fumarate or (S)-malate Escherichia coli mesaconate + H2O - r 444567 4.2.1.34 (S)-citramalate - Paraburkholderia xenovorans DSMZ 17367 / LB400 mesaconate + H2O - r 444567 4.2.1.34 (S)-citramalate the catalytic efficiency of FumA with (S)-citramalate/mesaconate is about 4% of that with fumarate or (S)-malate Escherichia coli W3110 / K-12 mesaconate + H2O - r 444567 4.2.1.34 (S)-citramalate - Escherichia coli W3110 / K-12 mesaconate + H2O - r 444567 4.2.1.34 (S)-malate - Escherichia coli fumarate + H2O - r 1970 4.2.1.34 (S)-malate - Clostridium tetanomorphum fumarate + H2O - ? 1970 4.2.1.34 (S)-malate - Aerobic bacterium fumarate + H2O - ? 1970 4.2.1.34 (S)-malate - Paraburkholderia xenovorans fumarate + H2O - r 1970 4.2.1.34 (S)-malate the catalytic efficiency of FumA with (S)-citramalate/mesaconate is about 4% of that with fumarate or (S)-malate Escherichia coli fumarate + H2O - r 1970 4.2.1.34 (S)-malate - Paraburkholderia xenovorans DSMZ 17367 / LB400 fumarate + H2O - r 1970 4.2.1.34 (S)-malate - Escherichia coli ATCC 700728 / O157:H7 fumarate + H2O - r 1970 4.2.1.34 (S)-malate the catalytic efficiency of FumA with (S)-citramalate/mesaconate is about 4% of that with fumarate or (S)-malate Escherichia coli W3110 / K-12 fumarate + H2O - r 1970 4.2.1.34 (S)-malate - Escherichia coli W3110 / K-12 fumarate + H2O - r 1970 4.2.1.34 Citramalate + H2O - Clostridium sp. Mesaconate - ? 1968 4.2.1.34 Citramalate + H2O - Clostridium tetanomorphum Mesaconate - ? 1968 4.2.1.34 Citramalate + H2O - Morganella morganii Mesaconate - ? 1968 4.2.1.34 Citramalate + H2O - Raoultella planticola Mesaconate - ? 1968 4.2.1.34 Citramalate + H2O - Aerobic bacterium Mesaconate - ? 1968 4.2.1.34 Citramalate + H2O - Citrobacter amalonaticus Mesaconate - ? 1968 4.2.1.34 Citramalate + H2O L-citramalate Clostridium tetanomorphum Mesaconate - ? 1968 4.2.1.34 Citramalate + H2O (+)-citramalate Clostridium tetanomorphum Mesaconate - ? 1968 4.2.1.34 Citramalate + H2O - Citrobacter amalonaticus YG-1002 Mesaconate - ? 1968 4.2.1.34 Citramalate + H2O - Citrobacter amalonaticus YG1002 Mesaconate - ? 1968 4.2.1.34 Citramalate + H2O - Morganella morganii NCIMB 232 Mesaconate - ? 1968 4.2.1.34 Citramalate + H2O - Raoultella planticola IAM 1133 Mesaconate - ? 1968 4.2.1.34 Citramalate + H2O - Morganella morganii NCIMB 9525 Mesaconate - ? 1968 4.2.1.34 Citramalate + H2O - Morganella morganii YG-0601 Mesaconate - ? 1968 4.2.1.34 Citramalate + H2O - Morganella morganii NCIMB 10466 Mesaconate - ? 1968 4.2.1.34 fumarate + H2O - Clostridium tetanomorphum L-malate - ? 1969 4.2.1.34 fumarate + H2O - Aerobic bacterium L-malate - ? 1969 4.2.1.34 fumarate + H2O - Paraburkholderia xenovorans (S)-malate - r 443574 4.2.1.34 fumarate + H2O heterologously produced FumD is a promiscuous mesaconase/fumarase with a 2- to 3-fold preference for mesaconate over fumarate Escherichia coli (S)-malate - r 443574 4.2.1.34 fumarate + H2O heterologously produced FumD is a promiscuous mesaconase/fumarase with a 2- to 3fold preference for mesaconate over fumarate Escherichia coli (S)-malate - r 443574 4.2.1.34 fumarate + H2O the catalytic efficiency of FumA with (S)-citramalate/mesaconate is about 4% of that with fumarate or (S)-malate Escherichia coli (S)-malate - r 443574 4.2.1.34 fumarate + H2O - Paraburkholderia xenovorans DSMZ 17367 / LB400 (S)-malate - r 443574 4.2.1.34 fumarate + H2O heterologously produced FumD is a promiscuous mesaconase/fumarase with a 2- to 3-fold preference for mesaconate over fumarate Escherichia coli ATCC 700728 / O157:H7 (S)-malate - r 443574 4.2.1.34 fumarate + H2O the catalytic efficiency of FumA with (S)-citramalate/mesaconate is about 4% of that with fumarate or (S)-malate Escherichia coli W3110 / K-12 (S)-malate - r 443574 4.2.1.34 fumarate + H2O heterologously produced FumD is a promiscuous mesaconase/fumarase with a 2- to 3fold preference for mesaconate over fumarate Escherichia coli W3110 / K-12 (S)-malate - r 443574 4.2.1.34 mesaconate - Clostridium tetanomorphum citramalate + H2O - ? 1967 4.2.1.34 mesaconate - Aerobic bacterium citramalate + H2O - ? 1967 4.2.1.34 mesaconate - Clostridium tetanomorphum citramalate + H2O (+)-citramalate ? 1967 4.2.1.34 mesaconate + H2O - Escherichia coli (S)-citramalate - r 444365 4.2.1.34 mesaconate + H2O - Paraburkholderia xenovorans (S)-citramalate - r 444365 4.2.1.34 mesaconate + H2O mesaconase activity of class I fumarase contributes to mesaconate utilization by Burkholderia xenovorans. Mesaconate is metabolized through its hydration to (S)-citramalate. The first reaction of the pathway, the mesaconate hydratase (mesaconase) reaction, is catalyzed by a class I fumarase. The latter compound is then metabolized to acetyl-CoA and pyruvate with the participation of two enzymes of the itaconate degradation pathway, a promiscuous itaconate-CoA transferase able to activate (S)-citramalate in addition to itaconate and (S)-citramalyl-CoA lyase Paraburkholderia xenovorans (S)-citramalate - ? 444365 4.2.1.34 mesaconate + H2O heterologously produced FumD is a promiscuous mesaconase/fumarase with a 2- to 3-fold preference for mesaconate over fumarate Escherichia coli (S)-citramalate - r 444365 4.2.1.34 mesaconate + H2O heterologously produced FumD is a promiscuous mesaconase/fumarase with a 2- to 3fold preference for mesaconate over fumarate Escherichia coli (S)-citramalate - r 444365 4.2.1.34 mesaconate + H2O the catalytic efficiency of FumA with (S)-citramalate/mesaconate is about 4% of that with fumarate or (S)-malate Escherichia coli (S)-citramalate - r 444365 4.2.1.34 mesaconate + H2O - Paraburkholderia xenovorans DSMZ 17367 / LB400 (S)-citramalate - r 444365 4.2.1.34 mesaconate + H2O mesaconase activity of class I fumarase contributes to mesaconate utilization by Burkholderia xenovorans. Mesaconate is metabolized through its hydration to (S)-citramalate. The first reaction of the pathway, the mesaconate hydratase (mesaconase) reaction, is catalyzed by a class I fumarase. The latter compound is then metabolized to acetyl-CoA and pyruvate with the participation of two enzymes of the itaconate degradation pathway, a promiscuous itaconate-CoA transferase able to activate (S)-citramalate in addition to itaconate and (S)-citramalyl-CoA lyase Paraburkholderia xenovorans DSMZ 17367 / LB400 (S)-citramalate - ? 444365 4.2.1.34 mesaconate + H2O - Escherichia coli ATCC 700728 / O157:H7 (S)-citramalate - r 444365 4.2.1.34 mesaconate + H2O heterologously produced FumD is a promiscuous mesaconase/fumarase with a 2- to 3-fold preference for mesaconate over fumarate Escherichia coli ATCC 700728 / O157:H7 (S)-citramalate - r 444365 4.2.1.34 mesaconate + H2O the catalytic efficiency of FumA with (S)-citramalate/mesaconate is about 4% of that with fumarate or (S)-malate Escherichia coli W3110 / K-12 (S)-citramalate - r 444365 4.2.1.34 mesaconate + H2O heterologously produced FumD is a promiscuous mesaconase/fumarase with a 2- to 3fold preference for mesaconate over fumarate Escherichia coli W3110 / K-12 (S)-citramalate - r 444365 4.2.1.34 additional information key enzyme of the mesaconate pathway of (S)-glutamate fermentation Clostridium sp. ? - ? 89 4.2.1.34 additional information constitutive enzyme, the enzyme may play a role in the interconversion between S-malate and fumarate in addition to that between S-citramalate and mesaconate Aerobic bacterium ? - ? 89