3.4.24.B11 aggrecan + H2O - ? - ? 15923 3.4.24.B11 aggrecan + H2O C-terminal spacer region of the enzyme is required for activity, substrate is a cartilage proteoglycan, cleavage site is the peptide bond Glu1871-Leu1872 within the substrates chondroitin sulfate attachment domain ? - ? 15923 3.4.24.B11 aggrecan + H2O substrate is a cartilage proteoglycan ? - ? 15923 3.4.24.B11 aggrecan + H2O in 50 mM Tris-HCl, pH 7.6, 150 mM NaCl and 5mM CaCl2 solution with 100 ng recombinant ADAMTS-1 at 37°C ? - ? 15923 3.4.24.B11 aggrecan + H2O 2mg/ml, assay at 37°C, 16 h ? - ? 15923 3.4.24.B11 aggrecan + H2O several cleavage sites are determined 11 aggrecan fragment product determination, overview ? 367543 3.4.24.B11 aggrecan + H2O cleavage site is Glu373-Ala374 2 aggrecan fragment - ? 367546 3.4.24.B11 alpha2-macroglobulin + H2O cleavage of the bait region of alpha2-macroglobulin, complex formation with alpha2-macroglobulin is dependent on the proteolytic activity ? - ? 37183 3.4.24.B11 biglycan + H2O biglycan is a small leucine-rich proteoglycan ? - ? 391508 3.4.24.B11 brevican + H2O clevage site is Glu395 ? - ? 367547 3.4.24.B11 brevican + H2O assay at 37°C, 3 h, 25 nM recombinant ADAMTS ? - ? 367547 3.4.24.B11 Collagen type I + H2O - ? - ? 16153 3.4.24.B11 desmocollin-3 + H2O - ? - ? 391818 3.4.24.B11 dystroglycan 1 + H2O - ? - ? 391897 3.4.24.B11 fluorescein-5(6)-carbonyl-Ala-Glu-Leu-Asn-Gly-Arg-Pro-Ile-Ser-Ile-Ala-Lys + H2O - ? - ? 469255 3.4.24.B11 Mac-2-binding protein + H2O - ? - ? 392521 3.4.24.B11 additional information enzyme inhibits fibroblast growth factor 2-induced vascularization in the cornea pocket assay and inhibits vascular endothelial growth factor-induced angiogenesis in the chorioallantoic membrane assay ? - ? 89 3.4.24.B11 additional information no activity with pro-collagen type I and III, collagen types I, II, IV, V, VIII and XVIII, fibronectin, laminin, vibronectin, and thrombospondin 1 ? - ? 89 3.4.24.B11 additional information vascular endothelial growth factor 165 and vascular endothelial growth factor 165 receptor tyrosine kinase are no substrates for proteolytic cleavage, but the enzyme blocks endothelial vascularization by inhibiting the necessary phosphorylation of the receptor ? - ? 89 3.4.24.B11 additional information enzyme has anti-aniogenic properties, processed form p65 shows altered affinity to heparin and endothelial cells, reduced activity upon suppression of endothelial cell proliferation ? - ? 89 3.4.24.B11 additional information enzyme inhibits endothelial cell proliferation, but not fibroblast or smooth muscle growth ? - ? 89 3.4.24.B11 additional information enzyme is genetically regulated by progesteron in the ovulation process ? - ? 89 3.4.24.B11 additional information enzyme plays a role in cell-cell and cell-matrix interactions, in cell adhesion and growth factor signaling ? - ? 89 3.4.24.B11 additional information interaction with heparin via the first TSP1 domain at the C-terminal part, enzyme shows an antiangiogenic effect, the enzyme is able to shed proteins from the surface of the endothelium ? - ? 89 3.4.24.B11 additional information vascular endothelial growth factor 165 and vascular endothelial growth factor 165 receptor tyrosine kinase are no substrates for proteolytic cleavage, but the enzyme blocks endothelial vascularization by inhibiting the necessary phosphorylation of the receptor, interaction with heparin via the first TSP1 domain at the C-terminal part, enzyme shows an antiangiogenic effect, the enzyme is able to shed proteins from the surface of the endothelium ? - ? 89 3.4.24.B11 additional information does not cleave laminin ? - ? 89 3.4.24.B11 nidogen-1 + H2O - ? - ? 385410 3.4.24.B11 nidogen-2 + H2O - ? - ? 392888 3.4.24.B11 Notch1 + H2O - ? - ? 382425 3.4.24.B11 peptide fragments of aggrecan + H2O peptide sequences contain the cleavage site of aggrecan at E373-A374 ? - ? 367542 3.4.24.B11 proteins + H2O - peptides - ? 367540 3.4.24.B11 proteins + H2O enzyme suppresses FGF2-induced vascularization in a cornea pocket assay and inhibited VEGF-induced angiogenesis in a chorioallantoic membrane assay peptides - ? 367540 3.4.24.B11 proteins + H2O rather restricted substrate specificity peptides - ? 367540 3.4.24.B11 proteins + H2O strict specificity for cleavage between glutamic acid and uncharged aliphatic amino acids in the core proteins of large aggregating proteoglycans peptides - ? 367540 3.4.24.B11 proteins + H2O enzyme is involved in the inflammatory process peptides - ? 367540 3.4.24.B11 syndecan-4 + H2O - ? - ? 385785 3.4.24.B11 syndecan-4 + H2O ADAMTS1 cleaves the ectodomain of syndecan-4. The shedding of syndecan-4 by ADAMTS1 disrupts cell adhesion and promotes cell migration ? - ? 385785 3.4.24.B11 syndecan-4 + H2O ADAMTS1 cleaves the ectodomain of syndecan-4 ? - ? 385785 3.4.24.B11 thrombospondin 1 + H2O cleavage site...TEENRELVSE/LKRPPL... ? - ? 415381 3.4.24.B11 thrombospondin-1 + H2O - 36000 Da fragment + 110000 Da fragment - ? 393664 3.4.24.B11 thrombospondin-2 + H2O - 42000 Da fragment + 30000 Da fragment - ? 393665 3.4.24.B11 tissue factor pathway inhibitor-2 + H2O - ? - ? 385834 3.4.24.B11 Type I collagen + H2O - ? - ? 16730 3.4.24.B11 versican + H2O - ? - ? 367541 3.4.24.B11 versican + H2O chondroitin sulphate proteoglycan-2 ? - ? 367541 3.4.24.B11 versican + H2O versican proteolysis in cardiac jelly at the canonical Glu441-Ala442 site is cooperatively mediated by ADAMTS1 and ADAMTS5 and required for proper ventricular cardiomyogenesis ? - ? 367541 3.4.24.B11 versican + H2O ADAMTS1 versicanase activity is approximately 1000fold lower than ADAMTS5 and 50fold lower than ADAMTS4 ? - ? 367541 3.4.24.B11 versican + H2O ADAMTS-1 cleaves versican at the Glu441-Ala442 bond to generate a 70000 Da fragment termed DPEAAE DPEAAE + ? - ? 419626 3.4.24.B11 versican isoform V0 + H2O a second cleavage site might be Glu405-Gln406, cleavage site is Glu1428-Ala1429 in the V0 isoform, activity with recombinant and native human substrate protein 2 peptides product identification ? 367545 3.4.24.B11 versican isoform V1 + H2O a second cleavage site might be Glu405-Gln406, cleavage site is Glu441-Ala442 in the V1 isoform, activity with recombinant and native human substrate protein 2 peptides product identification ? 367544