1.1.1.303	1,2-cyclohexanedione + NADH + H+	5% of the (R)-2,3-butanediol dehydrogenase activity with substrate acetoin	(R)-2-hydroxy-1-cyclohexanone + NAD+	-	407694	
1.1.1.303	2,3-pentanedione + NADH + H+	7% of the (R)-2,3-butanediol dehydrogenase activity with substrate acetoin	(3R)-3-hydroxy-2-pentanone + NAD+	-	407822	
1.1.1.303	diacetyl + NADH + H+	21% of the (R)-2,3-butanediol dehydrogenase activity with substrate acetoin	(R)-acetoin + NAD+	-	409283	
1.1.1.303	diacetyl + NADH + H+	51.4% of the (R)-2,3-butanediol dehydrogenase activity with substrate acetoin	(R)-acetoin + NAD+	-	409283	
1.1.1.303	diacetyl + NADH + H+	-	(R)-acetoin + NAD+	-	409283	
1.1.1.303	diacetyl + NADH + H+	stereoselective reduction	(R)-acetoin + NAD+	-	409283	
1.1.1.303	diacetyl + NADH + H+	60% activity compared to main activity of 2,3-butanediol dehydrogenase, i.e. oxidation of (2R,3R)-2,3-butanediol and reduction of (R)-acetoin, EC 1.1.1.4	(R)-acetoin + NAD+	-	409283	
1.1.1.303	additional information	enzyme is specific for NADH	?	-	89	
1.1.1.303	additional information	the two step reduction of diacetyl (butane-2,3-dione) with (R,R)-BDH solely yields (R,R)-butane-2,3-diol via (R)-acetoin, cf. EC 1.1.1.4. The enzyme catalyzes the (R)-specific oxidation of (R,R)- and meso-butane-2,3-diol to (R)- and (S)-acetoin with specific activities of 12 U/mg and 23 U/mg, respectively. Starting with diacetyl as a substrate, exclusively (R,R)-butane-2,3-diol is formed. Bacillus clausii BDH catalyzes a highly selective oxidation of secondary alcohol groups in (R)-configuration of butane-2,3-diols and also reduces the carbonyl function in acetoin and diacetyl in a stereoselective manner to yield the (R)-configuration of the resulting chiral center, it selectively acts on vicinal diketones, alpha-hydroxy ketones and vicinal diols	?	-	89