1.2.4.1 0.0048 - Y177A mutant enzyme, 2,6-dichlorophenolindophenol assay, substrate 2alpha-hydroxyethyl-thiamin diphosphate 348995 1.2.4.1 0.00574 - in cells grown on 50 mM glucose and 2 mM acetate, at 22°C 690077 1.2.4.1 0.00671 - Y177F mutant enzyme, 2,6-dichlorophenolindophenol assay, substrate 2alpha-hydroxyethyl-thiamin diphosphate 348995 1.2.4.1 0.0098 - wild-type enzyme, 2,6-dichlorophenolindophenol assay, substrate 2alpha-hydroxyethyl-thiamin diphosphate 348995 1.2.4.1 0.011 - in cells grown on 50 mM glycerol and 2 mM acetate, at 22°C 690077 1.2.4.1 0.0162 - recombinant His-tagged-alpha2,beta2 tetramer, assay with 2-hydroxyethylidene-thiamine diphosphate and 2,6-dichlorphenolindophenol 348983 1.2.4.1 0.0269 - recombinant His-tagged-alpha2,beta2 tetramer, assay with pyruvate and 2,6-dichlorphenolindophenol 348983 1.2.4.1 0.063 - decarboxylation in the absence of K3Fe(CN)6 as artificial electron acceptor 348983 1.2.4.1 0.087 - Y177A mutant enzyme, 2,6-dichlorophenolindophenol assay, substrate pyruvate 348995 1.2.4.1 0.13 - recombinant alpha2beta2 tetramer 348983